LOCUS       DARBER010000001       128948 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARBER010000001 DARBER010000000
VERSION     DARBER010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 128948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 128948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..128948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.001"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(12..1535)
                     /locus_tag="SCK06_000001"
     CDS             complement(12..1535)
                     /locus_tag="SCK06_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1150038.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="SCK06_000002"
     CDS             1787..2968
                     /locus_tag="SCK06_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1150039.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCK06_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCK06_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG1150040.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="SCK06_000004"
     CDS             complement(5053..7764)
                     /locus_tag="SCK06_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150041.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="SCK06_000005"
     CDS             complement(7774..8475)
                     /locus_tag="SCK06_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150042.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="SCK06_000006"
     CDS             8661..9260
                     /locus_tag="SCK06_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1150043.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="SCK06_000007"
     CDS             9341..10354
                     /locus_tag="SCK06_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1150044.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="SCK06_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="SCK06_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG1150045.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="SCK06_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="SCK06_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG1150046.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="SCK06_000010"
     CDS             11802..13175
                     /locus_tag="SCK06_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1150047.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="SCK06_000011"
     CDS             13532..15676
                     /locus_tag="SCK06_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1150048.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="SCK06_000012"
     CDS             complement(15728..16768)
                     /locus_tag="SCK06_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1150049.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="SCK06_000013"
     CDS             complement(17045..18847)
                     /locus_tag="SCK06_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150050.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="SCK06_000014"
     CDS             complement(18861..19613)
                     /locus_tag="SCK06_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150051.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="SCK06_000015"
     CDS             complement(19622..19984)
                     /locus_tag="SCK06_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1150052.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="SCK06_000016"
     CDS             complement(20160..21032)
                     /locus_tag="SCK06_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1150053.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="SCK06_000017"
     CDS             complement(21058..21570)
                     /locus_tag="SCK06_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG1150054.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="SCK06_000018"
     CDS             complement(21708..22499)
                     /locus_tag="SCK06_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG1150055.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="SCK06_000019"
     CDS             complement(22554..23162)
                     /locus_tag="SCK06_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG1150056.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="SCK06_000020"
     CDS             complement(23155..23472)
                     /locus_tag="SCK06_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1150057.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="SCK06_000021"
     CDS             complement(23628..24356)
                     /locus_tag="SCK06_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG1150058.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="SCK06_000022"
     CDS             24515..25396
                     /locus_tag="SCK06_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1150059.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="SCK06_000023"
     CDS             complement(25633..26676)
                     /locus_tag="SCK06_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1150060.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="SCK06_000024"
     CDS             complement(26679..27347)
                     /locus_tag="SCK06_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1150061.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="SCK06_000025"
     CDS             27489..29825
                     /locus_tag="SCK06_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1150062.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="SCK06_000026"
     CDS             complement(29940..30251)
                     /locus_tag="SCK06_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150063.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="SCK06_000027"
     CDS             30432..31907
                     /locus_tag="SCK06_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG1150064.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="SCK06_000028"
     CDS             31954..32538
                     /locus_tag="SCK06_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG1150065.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="SCK06_000029"
     CDS             32540..32959
                     /locus_tag="SCK06_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG1150066.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="SCK06_000030"
     CDS             complement(33240..33524)
                     /locus_tag="SCK06_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG1150067.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="SCK06_000031"
     CDS             complement(33876..34586)
                     /locus_tag="SCK06_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG1150068.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="SCK06_000032"
     CDS             complement(34583..36055)
                     /locus_tag="SCK06_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1150069.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="SCK06_000033"
     CDS             36193..36666
                     /locus_tag="SCK06_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1150070.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="SCK06_000034"
     CDS             complement(36819..37370)
                     /locus_tag="SCK06_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1150071.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="SCK06_000035"
     CDS             37475..38005
                     /locus_tag="SCK06_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG1150072.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="SCK06_000036"
     CDS             complement(38488..38931)
                     /locus_tag="SCK06_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1150073.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="SCK06_000037"
     CDS             complement(38947..40107)
                     /locus_tag="SCK06_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1150074.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="SCK06_000038"
     CDS             40614..42314
                     /locus_tag="SCK06_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150075.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="SCK06_000039"
     CDS             complement(42416..43249)
                     /locus_tag="SCK06_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG1150076.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="SCK06_000040"
     CDS             complement(43553..45718)
                     /locus_tag="SCK06_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1150077.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="SCK06_000041"
     CDS             45978..48272
                     /locus_tag="SCK06_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1150078.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="SCK06_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="SCK06_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1150079.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="SCK06_000043"
     CDS             48923..49930
                     /locus_tag="SCK06_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1150080.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="SCK06_000044"
     CDS             49952..50971
                     /locus_tag="SCK06_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1150081.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCK06_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCK06_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1150082.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="SCK06_000046"
     CDS             complement(52729..54150)
                     /locus_tag="SCK06_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1150083.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCK06_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCK06_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1150084.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="SCK06_000048"
     CDS             complement(56561..57817)
                     /locus_tag="SCK06_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG1150085.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="SCK06_000049"
     CDS             58397..59698
                     /locus_tag="SCK06_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1150086.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="SCK06_000050"
     CDS             complement(59767..60366)
                     /locus_tag="SCK06_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1150087.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="SCK06_000051"
     CDS             60620..61501
                     /locus_tag="SCK06_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150088.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="SCK06_000052"
     CDS             61514..63124
                     /locus_tag="SCK06_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG1150089.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="SCK06_000053"
     CDS             complement(63201..63860)
                     /locus_tag="SCK06_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1150090.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="SCK06_000054"
     CDS             complement(64143..64808)
                     /locus_tag="SCK06_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG1150091.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCK06_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCK06_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG1150092.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCK06_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCK06_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG1150093.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCK06_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCK06_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG1150094.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="SCK06_000058"
     CDS             67122..67442
                     /locus_tag="SCK06_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG1150095.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="SCK06_000059"
     CDS             complement(67554..68423)
                     /locus_tag="SCK06_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1150096.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="SCK06_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="SCK06_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="SCK06_000061"
     CDS             complement(69819..70328)
                     /locus_tag="SCK06_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150097.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="SCK06_000062"
     CDS             complement(70503..72638)
                     /locus_tag="SCK06_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1150098.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="SCK06_000063"
     CDS             complement(72721..74376)
                     /locus_tag="SCK06_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1150099.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="SCK06_000064"
     CDS             complement(74378..74692)
                     /locus_tag="SCK06_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1150100.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="SCK06_000065"
     CDS             74862..75122
                     /locus_tag="SCK06_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG1150101.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="SCK06_000066"
     CDS             75136..75450
                     /locus_tag="SCK06_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG1150102.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="SCK06_000067"
     CDS             complement(76180..76734)
                     /locus_tag="SCK06_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG1150103.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="SCK06_000068"
     CDS             complement(76860..78788)
                     /locus_tag="SCK06_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1150104.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="SCK06_000069"
     CDS             complement(79094..79810)
                     /locus_tag="SCK06_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1150105.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCK06_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCK06_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG1150106.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCK06_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCK06_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG1150107.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="SCK06_000072"
     CDS             complement(83280..84740)
                     /locus_tag="SCK06_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150108.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="SCK06_000073"
     CDS             complement(84730..86025)
                     /locus_tag="SCK06_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1150109.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="SCK06_000074"
     CDS             complement(86357..87034)
                     /locus_tag="SCK06_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG1150110.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="SCK06_000075"
     CDS             complement(87064..88155)
                     /locus_tag="SCK06_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG1150111.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="SCK06_000076"
     CDS             complement(88256..88690)
                     /locus_tag="SCK06_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1150112.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCK06_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCK06_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1150113.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="SCK06_000078"
     CDS             complement(90728..92623)
                     /locus_tag="SCK06_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1150114.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="SCK06_000079"
     CDS             complement(92681..94456)
                     /locus_tag="SCK06_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1150115.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="SCK06_000080"
     CDS             complement(94506..96002)
                     /locus_tag="SCK06_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1150116.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="SCK06_000081"
     CDS             complement(96196..97818)
                     /locus_tag="SCK06_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1150117.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="SCK06_000082"
     CDS             complement(98267..99580)
                     /locus_tag="SCK06_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1150118.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="SCK06_000083"
     CDS             complement(99825..100049)
                     /locus_tag="SCK06_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150119.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="SCK06_000084"
     CDS             complement(100069..100491)
                     /locus_tag="SCK06_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG1150120.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="SCK06_000085"
     CDS             complement(100635..101345)
                     /locus_tag="SCK06_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150121.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="SCK06_000086"
     CDS             complement(101363..101860)
                     /locus_tag="SCK06_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1150122.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="SCK06_000087"
     CDS             complement(101897..102349)
                     /locus_tag="SCK06_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1150123.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="SCK06_000088"
     CDS             complement(102484..103089)
                     /locus_tag="SCK06_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1150124.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="SCK06_000089"
     CDS             complement(103539..104159)
                     /locus_tag="SCK06_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1150125.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="SCK06_000090"
     CDS             complement(104161..105030)
                     /locus_tag="SCK06_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG1150126.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="SCK06_000091"
     CDS             complement(105053..105460)
                     /locus_tag="SCK06_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1150127.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="SCK06_000092"
     CDS             complement(105479..106768)
                     /locus_tag="SCK06_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1150128.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="SCK06_000093"
     CDS             complement(106795..107097)
                     /locus_tag="SCK06_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1150129.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="SCK06_000094"
     CDS             complement(107122..109215)
                     /locus_tag="SCK06_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1150130.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="SCK06_000095"
     CDS             complement(109704..110468)
                     /locus_tag="SCK06_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG1150131.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCK06_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCK06_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG1150132.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="SCK06_000097"
     CDS             complement(111473..112549)
                     /locus_tag="SCK06_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG1150133.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="SCK06_000098"
     CDS             complement(112573..113166)
                     /locus_tag="SCK06_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG1150134.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCK06_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCK06_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG1150135.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="SCK06_000100"
     CDS             complement(114836..115135)
                     /locus_tag="SCK06_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG1150136.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCK06_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCK06_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG1150137.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="SCK06_000102"
     CDS             complement(116890..117801)
                     /locus_tag="SCK06_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1150138.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="SCK06_000103"
     CDS             117921..118778
                     /locus_tag="SCK06_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1150139.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="SCK06_000104"
     CDS             complement(118965..119327)
                     /locus_tag="SCK06_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG1150140.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="SCK06_000105"
     CDS             119673..121112
                     /locus_tag="SCK06_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150141.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="SCK06_000106"
     CDS             complement(121119..123671)
                     /locus_tag="SCK06_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG1150142.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="SCK06_000107"
     CDS             complement(123785..125116)
                     /locus_tag="SCK06_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1150143.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="SCK06_000108"
     CDS             complement(125082..126560)
                     /locus_tag="SCK06_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1150144.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCK06_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCK06_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1150145.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        37741 a        26961 c        22547 g        41699 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaatg
//
LOCUS       DARBER010000002        87298 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARBER010000002 DARBER010000000
VERSION     DARBER010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.002"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCK06_000110"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCK06_000110"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG1150146.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCK06_000111"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCK06_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG1150147.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCK06_000112"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCK06_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG1150148.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCK06_000113"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCK06_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG1150149.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCK06_000114"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCK06_000114"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG1150150.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCK06_000115"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCK06_000115"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG1150151.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SCK06_000116"
     CDS             complement(8053..8523)
                     /locus_tag="SCK06_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG1150152.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SCK06_000117"
     CDS             complement(8569..8994)
                     /locus_tag="SCK06_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG1150153.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SCK06_000118"
     CDS             complement(9061..9330)
                     /locus_tag="SCK06_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG1150154.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="SCK06_000119"
     CDS             complement(9341..10936)
                     /locus_tag="SCK06_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1150155.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SCK06_000120"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SCK06_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG1150156.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="SCK06_000121"
     CDS             complement(14489..15049)
                     /locus_tag="SCK06_000121"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG1150157.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="SCK06_000122"
     CDS             15402..16376
                     /locus_tag="SCK06_000122"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1150158.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="SCK06_000123"
     CDS             16546..17946
                     /locus_tag="SCK06_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG1150159.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="SCK06_000124"
     CDS             complement(18024..18746)
                     /locus_tag="SCK06_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1150160.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="SCK06_000125"
     CDS             complement(18825..19331)
                     /locus_tag="SCK06_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1150161.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="SCK06_000126"
     CDS             19454..21253
                     /locus_tag="SCK06_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG1150162.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="SCK06_000127"
     CDS             complement(21321..22895)
                     /locus_tag="SCK06_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1150163.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SCK06_000128"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SCK06_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG1150164.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SCK06_000129"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SCK06_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG1150165.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="SCK06_000130"
     CDS             complement(24672..25310)
                     /locus_tag="SCK06_000130"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG1150166.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="SCK06_000131"
     CDS             complement(25311..25961)
                     /locus_tag="SCK06_000131"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG1150167.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SCK06_000132"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SCK06_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG1150168.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SCK06_000133"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SCK06_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG1150169.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="SCK06_000134"
     CDS             complement(29104..29844)
                     /locus_tag="SCK06_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG1150170.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SCK06_000135"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SCK06_000135"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG1150171.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="SCK06_000136"
     CDS             complement(31215..32153)
                     /locus_tag="SCK06_000136"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG1150172.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="SCK06_000137"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="SCK06_000137"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1150173.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SCK06_000138"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SCK06_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG1150174.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="SCK06_000139"
     CDS             complement(35313..35618)
                     /locus_tag="SCK06_000139"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1150175.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SCK06_000140"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SCK06_000140"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG1150176.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="SCK06_000141"
     CDS             complement(36724..37008)
                     /locus_tag="SCK06_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150177.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="SCK06_000142"
     CDS             complement(37026..37916)
                     /locus_tag="SCK06_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG1150178.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="SCK06_000143"
     CDS             complement(37936..38154)
                     /locus_tag="SCK06_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG1150179.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="SCK06_000144"
     CDS             complement(38124..39824)
                     /locus_tag="SCK06_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG1150180.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="SCK06_000145"
     CDS             40091..40864
                     /locus_tag="SCK06_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150181.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SCK06_000146"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SCK06_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG1150182.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="SCK06_000147"
     CDS             complement(41688..42659)
                     /locus_tag="SCK06_000147"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1150183.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SCK06_000148"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SCK06_000148"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG1150184.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SCK06_000149"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SCK06_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG1150185.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="SCK06_000150"
     CDS             complement(45614..46438)
                     /locus_tag="SCK06_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1150186.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="SCK06_000151"
     CDS             complement(46411..47091)
                     /locus_tag="SCK06_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1150187.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="SCK06_000152"
     CDS             complement(47114..48052)
                     /locus_tag="SCK06_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG1150188.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="SCK06_000153"
     CDS             complement(48297..49160)
                     /locus_tag="SCK06_000153"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG1150189.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="SCK06_000154"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="SCK06_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1150190.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="SCK06_000155"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="SCK06_000155"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG1150191.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="SCK06_000156"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="SCK06_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG1150192.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="SCK06_000157"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="SCK06_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG1150193.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="SCK06_000158"
     CDS             complement(56026..57267)
                     /locus_tag="SCK06_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG1150194.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="SCK06_000159"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="SCK06_000159"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG1150195.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="SCK06_000160"
     CDS             complement(58294..59217)
                     /locus_tag="SCK06_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150196.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="SCK06_000161"
     CDS             complement(59956..61242)
                     /locus_tag="SCK06_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1150197.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="SCK06_000162"
     CDS             complement(61235..62503)
                     /locus_tag="SCK06_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1150198.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="SCK06_000163"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="SCK06_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG1150199.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="SCK06_000164"
     CDS             complement(63276..63722)
                     /locus_tag="SCK06_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1150200.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="SCK06_000165"
     CDS             complement(63815..64060)
                     /locus_tag="SCK06_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG1150201.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64175..64753)
                     /locus_tag="SCK06_000166"
     CDS             complement(64175..64753)
                     /locus_tag="SCK06_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1150202.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64830..65360)
                     /locus_tag="SCK06_000167"
     CDS             complement(64830..65360)
                     /locus_tag="SCK06_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG1150203.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="SCK06_000168"
     CDS             complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="SCK06_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG1150204.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66217..66612)
                     /locus_tag="SCK06_000169"
     CDS             complement(66217..66612)
                     /locus_tag="SCK06_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG1150205.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66609..68021)
                     /locus_tag="SCK06_000170"
     CDS             complement(66609..68021)
                     /locus_tag="SCK06_000170"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG1150206.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68018..68554)
                     /gene="cysE"
                     /locus_tag="SCK06_000171"
     CDS             complement(68018..68554)
                     /gene="cysE"
                     /locus_tag="SCK06_000171"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG1150207.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(68640..68879)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(68876..70336)
                     /locus_tag="SCK06_000172"
     CDS             complement(68876..70336)
                     /locus_tag="SCK06_000172"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG1150208.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70506..71663)
                     /locus_tag="SCK06_000173"
     CDS             complement(70506..71663)
                     /locus_tag="SCK06_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG1150209.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71697..73067)
                     /gene="radA"
                     /locus_tag="SCK06_000174"
     CDS             complement(71697..73067)
                     /gene="radA"
                     /locus_tag="SCK06_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG1150210.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73130..73657)
                     /locus_tag="SCK06_000175"
     CDS             complement(73130..73657)
                     /locus_tag="SCK06_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG1150211.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73887..74693
                     /gene="proB"
                     /locus_tag="SCK06_000176"
     CDS             73887..74693
                     /gene="proB"
                     /locus_tag="SCK06_000176"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG1150212.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74690..75937
                     /locus_tag="SCK06_000177"
     CDS             74690..75937
                     /locus_tag="SCK06_000177"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG1150213.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76013..76357
                     /locus_tag="SCK06_000178"
     CDS             76013..76357
                     /locus_tag="SCK06_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150214.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76474..76800)
                     /locus_tag="SCK06_000179"
     CDS             complement(76474..76800)
                     /locus_tag="SCK06_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150215.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77415..78617)
                     /locus_tag="SCK06_000180"
     CDS             complement(77415..78617)
                     /locus_tag="SCK06_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1150216.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78734..79249
                     /locus_tag="SCK06_000181"
     CDS             78734..79249
                     /locus_tag="SCK06_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1150217.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79448..79894)
                     /locus_tag="SCK06_000182"
     CDS             complement(79448..79894)
                     /locus_tag="SCK06_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1150218.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79941..81419)
                     /gene="msr(C)"
                     /locus_tag="SCK06_000183"
     CDS             complement(79941..81419)
                     /gene="msr(C)"
                     /locus_tag="SCK06_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1150219.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81773..82402)
                     /locus_tag="SCK06_000184"
     CDS             complement(81773..82402)
                     /locus_tag="SCK06_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1150220.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82386..82850)
                     /locus_tag="SCK06_000185"
     CDS             complement(82386..82850)
                     /locus_tag="SCK06_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1150221.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83337..84605)
                     /locus_tag="SCK06_000186"
     CDS             complement(83337..84605)
                     /locus_tag="SCK06_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1150222.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84836..85573)
                     /locus_tag="SCK06_000187"
     CDS             complement(84836..85573)
                     /locus_tag="SCK06_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1150223.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85640..86209)
                     /locus_tag="SCK06_000188"
     CDS             complement(85640..86209)
                     /locus_tag="SCK06_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1150224.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86337..87209
                     /locus_tag="SCK06_000189"
     CDS             86337..87209
                     /locus_tag="SCK06_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1150225.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT        24247 a        18875 c        15676 g        28500 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaga aatgcaataa
    64141 aaataaataa gaagctcatt aagatttttc taaattattc ctcattataa aatttcaagt
    64201 gtttttttat tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg
    64261 ctaaacattc agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag
    64321 cttctatttt tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct
    64381 gaattgctaa aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta
    64441 ttttttggtc gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc
    64501 tcaacagaga atagatttga ttgcttataa cgcttttata ataacttcca aagctaacac
    64561 ctttgctttt ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc
    64621 aatcctcaga aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa
    64681 aatatttttg atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata
    64741 actcctcaga cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa
    64801 tgattcatcg aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat
    64861 ttttgggaca atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata
    64921 aaatgcatat ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat
    64981 aattcataag cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa
    65041 tcgcttgtcg ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg
    65101 atataactgt cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta
    65161 taattttgtt ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct
    65221 tcatatttcg cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg
    65281 tcctgctttt taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca
    65341 atcaacagct gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata
    65401 agcaaaccag cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc
    65461 agcaattcat ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg
    65521 actaaagcaa ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg
    65581 ccaaataccc aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg
    65641 cgtgcaacag ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt
    65701 ccaactgctc ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt
    65761 aaaatcgacc caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct
    65821 tcttttttct cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta
    65881 atcgcaagaa cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta
    65941 ggaacccatt tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt
    66001 tctcctcgtg aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct
    66061 actgcggcat ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa
    66121 actgactgat ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca
    66181 aatccacgtt tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt
    66241 ctgaatacac cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata
    66301 gcccatgagc gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc
    66361 gctcgtatgg cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa
    66421 aagatgttct tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt
    66481 cgttgccgca cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg
    66541 aacatagatt tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt
    66601 gtaatctctc atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc
    66661 cctttttctt tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt
    66721 gcttgatttc gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta
    66781 aataaaatac cgaagatcga aagccattgc gagaataaag cttctccttg tgccaagaca
    66841 gcggcagaaa ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc
    66901 actgtgatcc catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct
    66961 aaattttcta acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct
    67021 tggcggaatt tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct
    67081 ttcatcgtcg tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa
    67141 cgcaagactt gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga
    67201 gatttactca ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa
    67261 ttagcgaatt ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga
    67321 aattctaaat cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc
    67381 ataacggaac attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa
    67441 atctcgccag gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc
    67501 tgttcttccc ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt
    67561 aatttcccat aattttcaaa tttacgggta cggtaataca catcacctga agattcataa
    67621 gcatagcctt tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg
    67681 actcttggat gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct
    67741 cggatgaaac gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg
    67801 attttatcgt ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca
    67861 aaatagcgtc gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa
    67921 ttatacacag tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat
    67981 tcttcttttt ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt
    68041 ctcctttgat tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat
    68101 ctttcaacac aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta
    68161 atatctgcgc attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg
    68221 tttcttttcc tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt
    68281 ctgcggtttc gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg
    68341 ttgctcctgg atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg
    68401 ccaataaaaa gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat
    68461 gaaaaccagg gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct
    68521 ttgctgcatc aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt
    68581 ttatgtagga aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat
    68641 caaaagacgc tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc
    68701 taacgcctgt tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc
    68761 tgtgtttcgc caagaccact cccagaggca tttcggcaac cattcattga taccttgcac
    68821 cacccggtat ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt
    68881 tcagtacttg atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag
    68941 gcaattctgg accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt
    69001 ttccttttac gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat
    69061 ctactacttc catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt
    69121 caccagcaag tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg
    69181 tagacagatc aacgatttct gcagcatagc tcatttgagg ttgatacagg ctaacgatct
    69241 tcttcaccca ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta
    69301 gataaggcag acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt
    69361 ttacccattg cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat
    69421 cgaacatttt gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc
    69481 ccaataatgc aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt
    69541 cgataaattg caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga
    69601 tgatcaatgt catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca
    69661 gttgttttgg tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca
    69721 aatgatcatc gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttggatga
    69781 caaagtcgcc accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa
    69841 atgcgtattc tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt
    69901 ctttttctgc ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt
    69961 ctcctcttgc gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt
    70021 tgtatgctcg attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt
    70081 cagattgacg gtaagggccg tattctccag gattttcagg agattcatcc caattcattc
    70141 ccaaccaagc aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt
    70201 cagtatcctc aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga
    70261 ataatgcagt tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta
    70321 cgcgtacttt cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa
    70381 ttccgtcagt tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac
    70441 cttttctaag gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt
    70501 cttttttatt ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt
    70561 cctgcatgtg caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt
    70621 acaacgactt cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg
    70681 ccgtcatcaa ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca
    70741 ttcatcgttt caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta
    70801 agaacaggta cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg
    70861 acaccatcta aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca
    70921 aagtctccat cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata
    70981 tctaatccgc gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt
    71041 agttcgtaaa gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta
    71101 atatcataaa tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa
    71161 ttttctcctg attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg
    71221 atttttttgc ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt
    71281 ctcaatccca gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat
    71341 ggaatttcca tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc
    71401 gcaccgatcg caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact
    71461 tgtttgataa tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt
    71521 gcgcctaaca ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt
    71581 gcctgccaag ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc
    71641 aaagtaatga cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag
    71701 cggaacacac gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt
    71761 ggcgcttccc agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt
    71821 tggacttctt tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca
    71881 atttcaccga tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata
    71941 ctgacagcga tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt
    72001 aagtaagcat cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc
    72061 agagataccc gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta
    72121 ggtgtgacta atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg
    72181 atagcagagc cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg
    72241 acttcttcca atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat
    72301 cggtttttta ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac
    72361 accgtatcca ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca
    72421 tgaccgacga taaaaatggc tatcccattt gttttagcgg ttttgagtag ttctgcagtg
    72481 gtttctctta cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt
    72541 tggatggaat caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg
    72601 ttcatatctg tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg
    72661 cgtaatttta tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg
    72721 gctgctaatt gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca
    72781 ccaatcaaga ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct
    72841 aattttgttt tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt
    72901 gctttttctc ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct
    72961 tcgaccatcg tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca
    73021 tacccgcaat tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct
    73081 tttatcctaa tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg
    73141 aagaaccaaa tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg
    73201 gcaaaaagat cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa
    73261 agttggtgaa ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt
    73321 caatcacacc tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt
    73381 tagcaagctg caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg
    73441 ttggtttcaa ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt
    73501 ccaacccctt tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt
    73561 caaaatcata gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt
    73621 tatatttaga aataacttca aatcctcttt ttctcaccgg ttttcctccc taaataaact
    73681 actcctattc taacatatcc gaggctgtaa aagactttct ttacaaattc ttcaagaaat
    73741 tctgctatct taaagtaaac cattaattcg ttctttttcc aaacgactgc aagttgtaca
    73801 aaaaaactat tttttacatg actttactcg tcaaaacaag tagaatagaa cgaaattaat
    73861 gagtggatat ttgattgagg tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta
    73921 ttgtcaaagt aggaacaagt acattgattt atcccaatgg aaatatcaat cttgcggcaa
    73981 tcgatcagtt agcttttgtc ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag
    74041 tctcatcagg agctgtaggc gttggtatgc ataaattaca tttgtcagaa cgtccgactt
    74101 tgattcctga acagcaagct gcggcggctg ttggtcaagc cgaactgatg aatatctata
    74161 atcaaagatt cagtgtatat aatcaacaga cggcacagat cctcgtgaca agagacgtgg
    74221 tagattatcc tgaaagtcgt aaaaatgtga ggaatacatt ggaacagctt ttgattatgg
    74281 acacgatccc tatcgtgaac gaaaacgata ctgtagcagt ggatgaatta gatcatctga
    74341 caaaatttgg cgataatgat cagctgtcag ccatcatggc aaaacttgtc gcggcagacc
    74401 ttttgatcgt cctttctgat atcgacgggt tttattcaga taatccgact acaaatcccg
    74461 aggctcagat gtatcgctat atcgataaaa tcgatgaaaa gattctggca caagcaggtg
    74521 gtgctggtag cacatttgga acaggcggta tgcagagtaa attaaaagcc gcttctagga
    74581 tatttgaagc aaacagcgca atgattttag caaatggaaa aaatccggca attatttttg
    74641 atattcttgc aggaaaagaa gtaggtactt tatttaagga ggacttgaaa tgactgactt
    74701 gatgcaatta ggacaacagg caaaagatag cgcgcaacaa ctagcactta tgcctacaaa
    74761 ccgcaaaaat gagctacttg ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat
    74821 cattgaagca aatcataaag acttagaaaa agcagtcgaa aacaatatca gtgaaacgat
    74881 gcaagaccgc ttgcagctga cacctgagcg aatcaatgcc atggctacag agatcgaaaa
    74941 aattgcttcg ttagacgatc ctattggaaa agtagatgag atgtggacga acaccgacgg
    75001 attgagaatc ggcaaaaagc gcgtgccact aggcgtgatc ggcatcattt acgaatctcg
    75061 tccaaatgtc acgacagatg cagctagtct agcattcaaa tcagggaatg ccgttatcct
    75121 tcgcggtggc aaagaagcat tcttctcgaa tcaattatta gtccaattat tgcagcaagt
    75181 actggtcagt gctggcgaat ctccttatgc catccagttt gtcgatgata cttctcatga
    75241 aactgcagga cagttgatgc gattgacaga atacttagat gtgctgattc ctcgaggcgg
    75301 agcaaaatta atccaacgag tcaaagaaca ggcaacggta cctatcattg aaacaggtac
    75361 aggaaataat cacgtatata tcgataaaga agcacagtta tctatggcag tgaacatcat
    75421 cgtcaatgca aaagcttccc gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca
    75481 ttctgagatc gcaccttttt tcttgccagc tattgaaaaa gaactggtag aacacggggt
    75541 ctctttacgt gcggatgctc gtgcgttaga atatctagag acggctgctt tagcagaaga
    75601 cacagattgg gatactgaat atctagatta tatccttgct gtcaaagtgg tcgattcttt
    75661 ggatgaagca attgcacata tcaatcggca taatactaaa cattcagaaa cgatcgtgac
    75721 agatagttat gcggctagtc aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt
    75781 caatgcctcc actcgtttta cagacggatc ggtatttggt tttggtgcag agattggtat
    75841 ctctactcaa aagttgcatg cccgtggtcc aatggggtta aatgaattaa cttccacaaa
    75901 atatatcatt tatggcgatg gacaaatccg cgaataatgt ttcgtgtgaa acattattcc
    75961 cgccttttgc cttttttctg taaaggttct atccaactag aaaggtgtca atatgtttga
    76021 aattttaatg atttttttta tttaccttat ttctctgaat atcgctgctt ttctgggagt
    76081 cggtatttta tcccttttct ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa
    76141 atggtcgcag tattttgaca aaatcgggca aaaagggctg gttacacgct tgcacattag
    76201 ttacatggtt gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc
    76261 aatcgcttat acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca
    76321 gctgaataaa aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga
    76381 gtgccttgaa gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt
    76441 cacagcctct ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc
    76501 ctcttcgtta agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc
    76561 atccatggac aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg
    76621 ttcccgatac acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc
    76681 ttccgcattg attattggca attctttgat atagccacga taaatcaacc gatcatctga
    76741 aaattcaatg ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat
    76801 ctattatccc cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca
    76861 gtttcattat tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc
    76921 cgaataaatg gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat
    76981 gagaaactgt tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc
    77041 tcttggtaaa cggcgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata
    77101 tgagaagcta tttttccgtt ttgtttctct aatacttagg ataaaacgct gattttacaa
    77161 cctctgttaa acggtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat
    77221 atgaggaact atttttccgt ttgtttctct aatactcagg acaaaacgct gattttacaa
    77281 cctcctgata aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    77341 aatatgagaa gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    77401 caacctctga tttcttattc ttcttcattt gtagcaaaat aattacggtg ataaatgata
    77461 caacatacac tgataagaac aatggcacct gcaaaataaa acgcatagct ttcaccaata
    77521 tgttcaccaa tcacacctgc aataaatggt gctaaagctc caccgaaaaa gcggacaaaa
    77581 ctatagctgg acgaagcaac gtttcgttgc agacctggta tttccatagc aatcgtcgtc
    77641 aacagtgtat tgataattcc ttggaaaaat cctgctaaca caatgctgat cggtacaagg
    77701 actggcatag aagttccagc tcctaataac agcaaacaaa taatgaataa gacaaatgct
    77761 gctaaaattg tccgataagt cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa
    77821 gcaatagcca gaagcacacc ccagccaaaa aatacaaaac cgacttctaa ttcagaatag
    77881 ccaatcaata aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt atataacaat
    77941 gcgattagtc caactgaacg aagtttatga ttgcttaacg ctttgacacc ttcccaaagg
    78001 cctacttttc tctttggttt ctcagtttca ggtaaaagta ccgctagggc tataaaaccg
    78061 caaaacatca aaaatgctac cccaaaaaat ggataacgcc atgaaccact gcccaagacc
    78121 ccaccgacta gagggccaac agccattccg cagccaagtg ctgcttcata catcatgatt
    78181 gctttttctg tgttaccaac taaaatactg acgatcgttg ctaaagcagt agagacaaat
    78241 aaagcattcc cgacacccca gccggcacgt aacccaatca gcattccgat attcccagat
    78301 aatccgccaa gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc
    78361 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
    78421 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
    78481 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
    78541 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
    78601 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
    78661 caatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
    78721 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
    78781 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
    78841 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
    78901 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
    78961 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
    79021 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
    79081 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
    79141 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
    79201 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
    79261 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
    79321 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
    79381 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
    79441 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
    79501 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
    79561 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
    79621 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
    79681 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
    79741 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
    79801 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
    79861 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
    79921 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
    79981 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
    80041 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
    80101 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
    80161 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
    80221 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
    80281 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
    80341 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
    80401 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
    80461 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
    80521 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
    80581 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
    80641 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
    80701 agtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
    80761 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
    80821 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
    80881 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
    80941 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
    81001 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
    81061 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
    81121 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
    81181 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
    81241 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
    81301 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
    81361 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
    81421 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
    81481 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
    81541 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
    81601 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
    81661 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
    81721 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
    81781 acatagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
    81841 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
    81901 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
    81961 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
    82021 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
    82081 gcatcttccc tgattcgtaa gcttggttgt agatttcttc gccatattcg taacattgca
    82141 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
    82201 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
    82261 tcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
    82321 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
    82381 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
    82441 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
    82501 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctt gattttcttc
    82561 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
    82621 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
    82681 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
    82741 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
    82801 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
    82861 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
    82921 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
    82981 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
    83041 attgattctt atcaaactat agaagatagc tcttctgcag tagataattt tacaccgcca
    83101 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
    83161 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
    83221 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
    83281 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
    83341 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
    83401 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
    83461 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
    83521 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
    83581 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
    83641 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
    83701 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
    83761 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
    83821 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
    83881 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    83941 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    84001 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    84061 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    84121 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    84181 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    84241 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    84301 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    84361 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    84421 attgtatttt ctgagacagt agctgactgc aaagtacttt ctgtatcgtt ttcaattcct
    84481 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    84541 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    84601 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    84661 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttaat aaatttgctt
    84721 tttctgcata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    84781 gtcgtttgaa taactattta acaagtaatt ctctctggat attatccgtt atggattata
    84841 cttcctgtgt cgtcggacgg ataatcatgt tgctgacaga catccggtct ggcgtttcta
    84901 tcgcaaatgc tacagcagaa gctatatctt cagatgttaa tccccattct ttttgcgctt
    84961 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    85021 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    85081 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    85141 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    85201 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    85261 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    85321 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    85381 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    85441 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    85501 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    85561 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    85621 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    85681 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    85741 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    85801 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    85861 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    85921 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    85981 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    86041 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    86101 ctgctaaagg ctcttcgttt tccgcttgga tctgatggat cacttcaaag agcggatccg
    86161 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    86221 cttttatcct tactttataa cggacggctt atgatgtcta atacttataa aacatactta
    86281 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    86341 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    86401 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    86461 aacttggtgt gacacttttc actcgaggct cccgagagat acagttgacg gattctggtc
    86521 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    86581 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    86641 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    86701 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    86761 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    86821 aagatcgttg gggacttttg atacatcgga atcatccttt agcaaagcaa acagtcatcc
    86881 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    86941 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    87001 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    87061 ggatcatcaa tacttatggg acggaaaaag tattcatccc actcgatccg ccattacaag
    87121 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    87181 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    87241 taacaacgaa taagttaagc aaaagatcca atgattaaat aacataagtt tcttaatt
//
LOCUS       DARBER010000003        82297 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARBER010000003 DARBER010000000
VERSION     DARBER010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.003"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(553..2166)
                     /locus_tag="SCK06_000190"
     CDS             complement(553..2166)
                     /locus_tag="SCK06_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1150226.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="SCK06_000191"
     CDS             complement(2307..3215)
                     /locus_tag="SCK06_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1150227.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="SCK06_000192"
     CDS             3398..4522
                     /locus_tag="SCK06_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1150228.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="SCK06_000193"
     CDS             4749..5666
                     /locus_tag="SCK06_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1150229.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="SCK06_000194"
     CDS             5659..6492
                     /locus_tag="SCK06_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1150230.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7693
                     /locus_tag="SCK06_000195"
     CDS             6575..7693
                     /locus_tag="SCK06_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1150231.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7687..9585
                     /locus_tag="SCK06_000196"
     CDS             7687..9585
                     /locus_tag="SCK06_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG1150232.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9579..10802
                     /locus_tag="SCK06_000197"
     CDS             9579..10802
                     /locus_tag="SCK06_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1150233.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10888..12822)
                     /locus_tag="SCK06_000198"
     CDS             complement(10888..12822)
                     /locus_tag="SCK06_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1150234.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13048..13704
                     /locus_tag="SCK06_000199"
     CDS             13048..13704
                     /locus_tag="SCK06_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1150235.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13778..14131)
                     /locus_tag="SCK06_000200"
     CDS             complement(13778..14131)
                     /locus_tag="SCK06_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1150236.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14323..15252
                     /locus_tag="SCK06_000201"
     CDS             14323..15252
                     /locus_tag="SCK06_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG1150237.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15263..16099
                     /locus_tag="SCK06_000202"
     CDS             15263..16099
                     /locus_tag="SCK06_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG1150238.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16347..17123
                     /locus_tag="SCK06_000203"
     CDS             16347..17123
                     /locus_tag="SCK06_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1150239.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17299..18042
                     /locus_tag="SCK06_000204"
     CDS             17299..18042
                     /locus_tag="SCK06_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150240.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18035..19615
                     /locus_tag="SCK06_000205"
     CDS             18035..19615
                     /locus_tag="SCK06_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG1150241.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19866..20345)
                     /locus_tag="SCK06_000206"
     CDS             complement(19866..20345)
                     /locus_tag="SCK06_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1150242.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20361..21113)
                     /locus_tag="SCK06_000207"
     CDS             complement(20361..21113)
                     /locus_tag="SCK06_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1150243.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21230..21300
                     /locus_tag="SCK06_000208"
     tRNA            21230..21300
                     /locus_tag="SCK06_000208"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21262..21264,aa:Gly,seq:ccc)
     gene            21661..22386
                     /locus_tag="SCK06_000209"
     CDS             21661..22386
                     /locus_tag="SCK06_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1150244.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22641..22907
                     /locus_tag="SCK06_000210"
     CDS             22641..22907
                     /locus_tag="SCK06_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG1150245.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22904..23335
                     /gene="ruvX"
                     /locus_tag="SCK06_000211"
     CDS             22904..23335
                     /gene="ruvX"
                     /locus_tag="SCK06_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG1150246.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23360..23665
                     /locus_tag="SCK06_000212"
     CDS             23360..23665
                     /locus_tag="SCK06_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG1150247.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23746..24891)
                     /locus_tag="SCK06_000213"
     CDS             complement(23746..24891)
                     /locus_tag="SCK06_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1150248.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24952..25599)
                     /locus_tag="SCK06_000214"
     CDS             complement(24952..25599)
                     /locus_tag="SCK06_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1150249.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25793..26086
                     /locus_tag="SCK06_000215"
     CDS             25793..26086
                     /locus_tag="SCK06_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150250.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26130..26441
                     /locus_tag="SCK06_000216"
     CDS             26130..26441
                     /locus_tag="SCK06_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150251.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26438..27062
                     /locus_tag="SCK06_000217"
                     /pseudo
     CDS             26438..27062
                     /locus_tag="SCK06_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27203..27361
                     /locus_tag="SCK06_000218"
     CDS             27203..27361
                     /locus_tag="SCK06_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150252.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27362..27724
                     /locus_tag="SCK06_000219"
     CDS             27362..27724
                     /locus_tag="SCK06_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150253.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27761..28609
                     /locus_tag="SCK06_000220"
     CDS             27761..28609
                     /locus_tag="SCK06_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEG1150254.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28599..30074
                     /locus_tag="SCK06_000221"
     CDS             28599..30074
                     /locus_tag="SCK06_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEG1150255.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30340..30747
                     /locus_tag="SCK06_000222"
     CDS             30340..30747
                     /locus_tag="SCK06_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEG1150256.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30750..30965
                     /locus_tag="SCK06_000223"
     CDS             30750..30965
                     /locus_tag="SCK06_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150257.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30969..31349
                     /locus_tag="SCK06_000224"
     CDS             30969..31349
                     /locus_tag="SCK06_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1150258.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31483..31638
                     /locus_tag="SCK06_000225"
     CDS             31483..31638
                     /locus_tag="SCK06_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEG1150259.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31707..32180
                     /locus_tag="SCK06_000226"
     CDS             31707..32180
                     /locus_tag="SCK06_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEG1150260.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32177..33871
                     /locus_tag="SCK06_000227"
     CDS             32177..33871
                     /locus_tag="SCK06_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1150261.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33837..34022
                     /locus_tag="SCK06_000228"
     CDS             33837..34022
                     /locus_tag="SCK06_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150262.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34026..35201
                     /locus_tag="SCK06_000229"
     CDS             34026..35201
                     /locus_tag="SCK06_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1150263.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35194..36717
                     /locus_tag="SCK06_000230"
     CDS             35194..36717
                     /locus_tag="SCK06_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1150264.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36773..37057
                     /locus_tag="SCK06_000231"
     CDS             36773..37057
                     /locus_tag="SCK06_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1150265.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37044..37379
                     /locus_tag="SCK06_000232"
     CDS             37044..37379
                     /locus_tag="SCK06_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1150266.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37674..38300
                     /locus_tag="SCK06_000233"
     CDS             37674..38300
                     /locus_tag="SCK06_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150267.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39042..39879
                     /locus_tag="SCK06_000234"
                     /pseudo
     CDS             39042..39879
                     /locus_tag="SCK06_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            39944..40378
                     /locus_tag="SCK06_000235"
     CDS             39944..40378
                     /locus_tag="SCK06_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1150268.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40446..41768)
                     /locus_tag="SCK06_000236"
     CDS             complement(40446..41768)
                     /locus_tag="SCK06_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG1150269.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41922..43562)
                     /locus_tag="SCK06_000237"
     CDS             complement(41922..43562)
                     /locus_tag="SCK06_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG1150270.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43892..44362
                     /locus_tag="SCK06_000238"
     CDS             43892..44362
                     /locus_tag="SCK06_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG1150271.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44991..46094
                     /locus_tag="SCK06_000239"
     CDS             44991..46094
                     /locus_tag="SCK06_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1150272.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46378..47058
                     /locus_tag="SCK06_000240"
     CDS             46378..47058
                     /locus_tag="SCK06_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1150273.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47148..48182
                     /locus_tag="SCK06_000241"
     CDS             47148..48182
                     /locus_tag="SCK06_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1150274.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48196..48531
                     /locus_tag="SCK06_000242"
     CDS             48196..48531
                     /locus_tag="SCK06_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150275.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48515..50995
                     /locus_tag="SCK06_000243"
     CDS             48515..50995
                     /locus_tag="SCK06_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150276.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51051..52610
                     /locus_tag="SCK06_000244"
     CDS             51051..52610
                     /locus_tag="SCK06_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150277.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52607..53971
                     /locus_tag="SCK06_000245"
     CDS             52607..53971
                     /locus_tag="SCK06_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1150278.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54142..55212
                     /locus_tag="SCK06_000246"
     CDS             54142..55212
                     /locus_tag="SCK06_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150279.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55227..55895
                     /locus_tag="SCK06_000247"
     CDS             55227..55895
                     /locus_tag="SCK06_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150280.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55915..56493
                     /locus_tag="SCK06_000248"
     CDS             55915..56493
                     /locus_tag="SCK06_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1150281.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56896..57807
                     /locus_tag="SCK06_000249"
     CDS             56896..57807
                     /locus_tag="SCK06_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1150282.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58063..58980)
                     /locus_tag="SCK06_000250"
     CDS             complement(58063..58980)
                     /locus_tag="SCK06_000250"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG1150283.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59182..59619
                     /gene="zapA"
                     /locus_tag="SCK06_000251"
     CDS             59182..59619
                     /gene="zapA"
                     /locus_tag="SCK06_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG1150284.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59677..60224
                     /locus_tag="SCK06_000252"
                     /pseudo
     CDS             59677..60224
                     /locus_tag="SCK06_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            60339..62699
                     /locus_tag="SCK06_000253"
     CDS             60339..62699
                     /locus_tag="SCK06_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1150285.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62786..63100
                     /gene="trxA"
                     /locus_tag="SCK06_000254"
     CDS             62786..63100
                     /gene="trxA"
                     /locus_tag="SCK06_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG1150286.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63282..65060
                     /gene="uvrC"
                     /locus_tag="SCK06_000255"
     CDS             63282..65060
                     /gene="uvrC"
                     /locus_tag="SCK06_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG1150287.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65205..66104
                     /locus_tag="SCK06_000256"
     CDS             65205..66104
                     /locus_tag="SCK06_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150288.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66097..67359
                     /locus_tag="SCK06_000257"
     CDS             66097..67359
                     /locus_tag="SCK06_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150289.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67522..69420
                     /locus_tag="SCK06_000258"
     CDS             67522..69420
                     /locus_tag="SCK06_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG1150290.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69719..70438)
                     /locus_tag="SCK06_000259"
     CDS             complement(69719..70438)
                     /locus_tag="SCK06_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG1150291.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            70775..71107
                     /locus_tag="SCK06_000260"
     CDS             70775..71107
                     /locus_tag="SCK06_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150292.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71094..73097
                     /locus_tag="SCK06_000261"
     CDS             71094..73097
                     /locus_tag="SCK06_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG1150293.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73116..73586
                     /locus_tag="SCK06_000262"
     CDS             73116..73586
                     /locus_tag="SCK06_000262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG1150294.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73607..74191
                     /locus_tag="SCK06_000263"
     CDS             73607..74191
                     /locus_tag="SCK06_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG1150295.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74206..75192
                     /locus_tag="SCK06_000264"
     CDS             74206..75192
                     /locus_tag="SCK06_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG1150296.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75182..75493
                     /locus_tag="SCK06_000265"
     CDS             75182..75493
                     /locus_tag="SCK06_000265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG1150297.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75515..77296
                     /locus_tag="SCK06_000266"
     CDS             75515..77296
                     /locus_tag="SCK06_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG1150298.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77289..78665
                     /locus_tag="SCK06_000267"
     CDS             77289..78665
                     /locus_tag="SCK06_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG1150299.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78672..79307
                     /locus_tag="SCK06_000268"
     CDS             78672..79307
                     /locus_tag="SCK06_000268"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG1150300.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79337..80692
                     /locus_tag="SCK06_000269"
     CDS             79337..80692
                     /locus_tag="SCK06_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG1150301.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80884..82278)
                     /locus_tag="SCK06_000270"
     CDS             complement(80884..82278)
                     /locus_tag="SCK06_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1150302.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT        27237 a        13900 c        16939 g        24221 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattccgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgccaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     7561 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     7621 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     7681 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     7741 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     7801 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     7861 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     7921 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     7981 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     8041 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     8101 agccagatta ttcattggtt ctcgcaaatt tttgaatatg aatgtgcaga tggtaattgg
     8161 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     8221 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     8281 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     8341 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     8401 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     8461 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     8521 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     8581 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     8641 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     8701 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     8761 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     8821 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     8881 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     8941 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     9001 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     9061 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     9121 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     9181 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     9241 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     9301 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     9361 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     9421 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     9481 tatagtttag aagtatggaa caatcgtccc aaaattgatt ttgatgggac aaccgggcta
     9541 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     9601 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     9661 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     9721 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     9781 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     9841 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     9901 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     9961 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
    10021 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
    10081 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
    10141 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
    10201 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
    10261 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
    10321 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
    10381 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
    10441 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
    10501 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
    10561 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
    10621 ggaaaattat gcgacaattg aaagaaagat atgctacaac ggaagaagaa gatggctttt
    10681 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
    10741 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
    10801 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
    10861 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
    10921 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
    10981 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
    11041 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
    11101 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
    11161 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
    11221 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
    11281 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
    11341 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
    11401 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
    11461 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
    11521 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
    11581 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
    11641 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
    11701 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
    11761 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
    11821 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
    11881 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
    11941 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
    12001 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
    12061 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
    12121 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
    12181 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
    12241 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
    12301 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    12361 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    12421 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    12481 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    12541 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    12601 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    12661 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    12721 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    12781 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    12841 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    12901 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    12961 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    13021 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    13081 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    13141 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    13201 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    13261 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    13321 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    13381 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    13441 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    13501 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    13561 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    13621 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    13681 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    13741 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    13801 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    13861 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    13921 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    13981 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    14041 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    14101 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    14161 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    14221 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    14281 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    14341 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    14401 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    14461 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    14521 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    14581 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    14641 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    14701 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    14761 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    14821 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    14881 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    14941 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    15001 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    15061 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    15121 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    15181 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    15241 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    15301 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    15361 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    15421 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    15481 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    15541 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    15601 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    15661 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    15721 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    15781 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    15841 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    15901 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    15961 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    16021 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    16081 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    16141 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    16201 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    16261 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    16321 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    16381 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    16441 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    16501 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    16561 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    16621 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    16681 tgataaaaga attcacttat gaaaaaaata aaaaaagcat ccaaagttac ttattgaaag
    16741 aaaaagaaaa ctatcctgtt caacatgcta cgaaaaaaag gtggacaact gtccgttatg
    16801 gtaatagcga tcactatatc cattacgatg aaatccttta tctaatgagc atgccaggtt
    16861 ataaaaataa aataggccta gtattgaaag atgaagagat tctcgtaaac ggcacaatta
    16921 aaaaatataa aaaatcactc gaatatgagt atttatgtaa agatttaaaa gcttatatca
    16981 ttaatactga aaacattaca tctattagca gagtaaacga aatagtaaca tttgcaaatg
    17041 gagaacaact agagatggga aagagaagta ttgataaact aaagaatttt atgcaagaaa
    17101 agatagaaaa tgaatcattt taaagaaaac aaagactcat atcttacctg ttagaagagt
    17161 gttttcctgt agaagagaac aacccaagta gcagcctagt cgtttctatc cttttttgtt
    17221 atgcactctt tttgacattt actccaccaa taacgaaggg tacaataaaa agtaataaca
    17281 aacaaaagga gtacagaaat gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa
    17341 caagcattaa aagacttgtc tctctcgata agacaagggg aaatttttgg atttttagga
    17401 cataatggtg ccggaaaatc aactacgatt aaaagtttag ttagtattat tgaaccatca
    17461 tcgggaacga ttttagtaga tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa
    17521 aaaatcggtt atgtgcctga ttccccagat atttttttac aattgacggc tggagaatat
    17581 tgggacttga tttcagctgc ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca
    17641 gaattgacag cgctttttga tatgtatagt catcaaaatg aaacaatcgc aagtttttca
    17701 catggaatgc gtcaaaaaac gattttgatc ggcacgttgc tgccagatcc agatatttgg
    17761 gtattggatg aacctttgca aggacttgat cctcaagcag catttgattt gaaagaaatg
    17821 atgaaggctc atgccgcaaa aggaaaaaca gtcatttttt ctacacatgt attggatacc
    17881 gcacaacagt tatgcgataa actggctatt ttgaagaaag gtgaattgat ttatcagggg
    17941 gcagttagtg aattattgaa tggctcacct gatgaaacgc tagaaaagat ttatttgaaa
    18001 atggctggaa ggcaagcgac aggagatgga ccgtatgtat aagcgacaat tattagaatt
    18061 aattcgagtt aatcttttat atgttaatcc acagctcacg aataaagcaa gagagaaagg
    18121 aaaaaaggga aagcgattaa ctatctatct tttgtctcaa aatctttttt cagggctgat
    18181 ttttttactg atttacggat ttgctatgtt tgctatgaac tttgttaaat tgccaggttt
    18241 ctttacttat tacgtggcac tttttggtat gttaggttta actcaaagta tatcagtcat
    18301 ttataatatc ttttttgaag gaaatgacct gcaagtattt ttgcctctgc cttttggaca
    18361 agaacaggtt ttttcagcaa agattttggt agttgctttg accatcattc cttttgtttt
    18421 tccgcttcta gtcatgttta ttttaaccgg atggcgagct gatctaggga ttttgcttac
    18481 tatcattatc tcaatccttt tgttttctct atttttgatt attctttttt gtctggctag
    18541 tttgattgtt tttggtttgg ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc
    18601 tagtatcttg cttggcagct cgatgctgat cgcatttgct ggaatcatgt ggatgaatca
    18661 tcaaacgggg actattgaaa atgagttaac agatcggcat ccgatttcga tattattgcc
    18721 attctatgat gctgccagtc agccactttc tcaacaaggt ttattgggtt ggggaatact
    18781 agctatcacg gctctgggat ttttccttgc tgtaaaacag ctgatagtac caaagcttac
    18841 agagcaactg actgattcaa acagtgagtt caaaataaaa agaacccata aaaagaacca
    18901 aaatgtgcat cagcttttat ttagttacaa tgctcaatta gtaaaagaac ctaatttgat
    18961 tatgcaagtt ctttcttctt ccttgttaac tccaatcatt tttattcttg cttttgccct
    19021 aggaggcgaa ataaaattaa ctgatttaga taataagtta atcggtgttg ttttcctggt
    19081 agggatcgca ttggctttct taacagttaa tcagacttct tttatcagca atcttatttc
    19141 tttggatcaa gaaaattttc tgtttatccg ttctttgcca atctctatga accaatattt
    19201 aaaagaaaag ttcagattcg gatggatatt gcagagtttt ttaactggtg ggatcgcatt
    19261 attaagcgga ctgtcttttc agctgccttt gttttttatc cctagcttgc tatttggttc
    19321 tttattcggc tgctacttat tgtcactacg ttatttcgct agagattatc ggttgctgct
    19381 gctgaactgg accaatatta accaattatt taacagaggt tcaggaagtt tgggtttagt
    19441 cgcaactatg atgggttcat tgatcatcag tattattctt atcgtcttat acggaatgtt
    19501 ggctttgttt tatccatttt ggttagtgaa tattcctgtg atcggtttag ttctaatact
    19561 ttccgctcta tggatcgttt attatcaacg aacgttttgg aaaaaatttg aataaagaag
    19621 aggggccgtg acaaaaatca tgtcgcagcc ccttttttac agatagaggg tgttcaaaag
    19681 tcagtttctc agtattaagt caattttatg taaaaatgtg agaagctatt tttacaaaac
    19741 tttttttaat actcataatt acttcttatt gctcctgcgg ttacttgtcg caatgtttgt
    19801 gagatatttt gtcatcgctc aaggctgaac gtctctttca caaccttttc tttgttacac
    19861 ttcgtttatt taaaaagccg tttttttcgt ttcatcatga ttgcgtcacg ctttactttg
    19921 aaattacgga tgttatggtt aaagacttct gataatacaa gacctaaagc gattgctcca
    19981 gcaatcataa ttgcttggac cgcataatga gccgcttctc gataggattc agtaacgaga
    20041 tttcgaaccg cttgataagc taaaccacca gggactagag gaaccatccc gggaatattg
    20101 aaaatcgtta cgggcatttt caaacgttta gagaaaagat cgctgatgaa tgctactcct
    20161 aaagaaccaa gcaatgaacc aaaagcagca gatccgccta tttgaacaca gatccagtag
    20221 atcatccagc caaaagcacc agaaagacca caacaaatca atgaacgcct aggtacattt
    20281 gtaataacgg caaatgccgc agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc
    20341 cccatacttg tgttcctttc ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg
    20401 atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca
    20461 gctagcaaat cacggatagc gttcgtaatt tgaactccag gaactaaagg catcacacat
    20521 ccgataatga ttagatcctg attgcttcct aagccaaaac gtacgctgta gatagcggct
    20581 atgccaatca ataaagaaga taaaaattcc gataaaaatt tgattctaaa aaatttcaaa
    20641 ctatatagat aagctgcgta tcccaatcct ccaatgacta aagtcacagg taaatcggtc
    20701 atctttccgc caaataacac cataattgtt ccactgatca ctgccgcgct aacgatttgc
    20761 aaccagacag gaaagaaaga acggtctcgg tcgattgctt gcagttcgca gtaaagttct
    20821 tctaatgtta gctctgaagc aacatattta cgtgaaagat catttacttt tactactttt
    20881 tctaaattaa tcgaacggtt ggtaatttgt tccattcgga tggtcgaggt acgatcaaga
    20941 ccaataaaga gaccggtttg agtgacatag ctgaccaaac ggaaatttcc agaagctaaa
    21001 gcaattcgac tcatcgtatc ttctacccga tacatctccg cattgctttc catcatgatt
    21061 ttcccagcga gtaaacaagt gtccagcaac agatctaatt cttttcccgt catagcatct
    21121 tctccaataa ttgattgatt agttttattt tactacaaaa cataccagaa gcgtaaagaa
    21181 taaaaaaact tgaggaagga ataaaagagg cgtatactgt ttttgtattg cgggtgtagt
    21241 ttagtggtaa aatcccagct tcccaagctg atgtcgcggg ttcgattccc gtcacccgct
    21301 taaaataggt tgtgaaaaag ctgctctgca acaagtaata agaacagcca agcaaaaaca
    21361 acttcttatg tttttaactt ggatggactt attaccgcag atgcaagctt ttgcgcaccg
    21421 tttaccaagc ggttgtgaaa tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa
    21481 taataaatac cttacagata gagtggtgac tgattagaga cgaggaatcc gtctcttttt
    21541 tgtattttta agccatctta aaacaggttt tattttttgc tgtttctatt tctttctagt
    21601 atttatttat aggggaacat atgttaagat ttagggagct gttagtaaag gagtaaagaa
    21661 atgagcaaaa aaacaaccaa ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca
    21721 ttcggtgttt ccaccacaca aaaagattca gatatctttc agcagattac aactattttt
    21781 gattccacac cacaagcttc tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa
    21841 gaaatagaaa tcaatgggaa tcagccggaa tttacaaaag aggatcttag cctttccaaa
    21901 ggaacatggg aaagctattc aaatattgat aaattgaaca gagtaggaca agccaatgct
    21961 atgcttggaa aagatttgtt ccctactgaa aagagagagc ctttgtatat tgacccgaca
    22021 ggttggaaac aaaagaaatt aagtgacggg caatggttat ataatcgcag ccatttaatt
    22081 ggtttccagc tgactggtca aaataacaac attaaaaact tgatgacagg gacacgttcg
    22141 ttgaatgctc catacatgct tgctcacgaa aatgatattg cggcatatat caaagaaaca
    22201 aatcatcatg tacgctaccg tgtcactcct cattttgagg ggaatgaact ggtagccagg
    22261 ggagtgcaat tggaagcaga aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc
    22321 tacaatgttc aagatggcta cacgatcaat tatgagacag gacaagcaac aaaaacaaaa
    22381 aattagtagc agctattcga attttttatg aatattatga aaaaattgtg taaaaacata
    22441 cattcaaaga aatatagcgt atactttatg aaaagagtac ttttgataaa gctacagaaa
    22501 aaaggaaaaa aacatacaaa tagtgtttct taatttattc ggaagtttgg aatatttgtt
    22561 tgtagttttc cctttcttcg agtaaaataa ggttgtagaa ggtaagctgg aattaccttg
    22621 cgggaaagag gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg
    22681 atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa
    22741 aaggctacaa tccaatcaat cagattgtcg gctacttgct ttctggagac ccggcctaca
    22801 ttcctcgtta tcgtgatgct cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg
    22861 aagtaattgt caaggactac ctctcaaatc atggagtgaa tttatgagag taatgggctt
    22921 ggatgtcggt tctaagacag taggtgttgc tatcagtgac ccctttggct ggacagctca
    22981 aggagtagag atcattcgga tcaatgaagc agaagaagag tttggctttg atcggttgaa
    23041 ggaattagta gcacaatatg aagtagaccg gtttgtagtt ggattaccta aaaatatgaa
    23101 caattccatc ggaccaaggg cagaagcttc catgtcatac ggagaaaaga tcaaagaact
    23161 attctccttg ccagttgatt atcaagatga acgattaacc actgttcagg cagaacgtat
    23221 gctagtagag caggcaaata cttcaagagc caaacgaaaa aaagtcatcg ataaattagc
    23281 agctgtgatg atcttacaaa attatttaga cggctcaggt aagcaattat tttaataata
    23341 aaagaaaaga ggtaatctta tgacagaaca taaccattcc cacgaccatg aaggacacga
    23401 acacatcaca ttggtggatg accaaggaaa tgaaacatta tatgaaatcc tactaacagt
    23461 tgacggacaa gaagaatttg gacgtaacta tgttctttta tatcctgctg gtgtttcaga
    23521 agatgaagat gtagaattac aagcttatgc atacatcgaa aacgaagatg gaacagaagg
    23581 cgagttagaa caaatcgaaa cagacgcaga atgggacatg attgaagaag tattcaatac
    23641 attcatggcg gaagaagaag aatagactat aaagctagga gaaaccttga tatgataggg
    23701 tttctccttt ttttattttt tactgtatgc catattgtat gccatctagt caatatttaa
    23761 gtgttgtact aatttttcaa cagtcttttc tttcgtttct tttgccaagt gaccgtaaca
    23821 atcgatcgtc tgactaattt ttgtatgccg taatctagtt tgaacttcta ccatagtagc
    23881 tccagcgtcc aagagcattg aagcatgagt gtgtcttgct acatgcacac cgagaaatgg
    23941 aaccatagct ttttcacaaa tagtaatcaa tcttgcataa atagcgtggt gtgtgattag
    24001 ttctttatag ataccacaaa aaatcatagt cggatcagtt acaccaagtg tcagcatata
    24061 tttccgctga ttaatctgcc atttttttaa tatagaaatt gttctttcat ctagtggaag
    24121 aaccccagca ctacaatcag ttttaggatc actgatttta tagccatttt tggattggct
    24181 tagtgtttta ttgatggtga ccgtattttc ttgaaaatca atgtctgacc aattcaatgc
    24241 aagggcttca ctgatcctaa ccccgctata aaagagaaag cgtagtaacg ttttatcaaa
    24301 gtcgtttcga taagtttcag gtttgctgtt taggtaatca aacaaggtga tcagttgttc
    24361 tttactgtaa actttgacct tttctttatg cttagtggtg gttcttttag gcattaatac
    24421 gttgtctagt ggattagtct caataatccc aagattgata gcgtagctaa aaatacgctt
    24481 agaagttgaa tgaagtacct tgtattggtc agtagtttca gcccagctat ttacaatttg
    24541 ttgggctttt tttaccgtga cttcattcac acgaagattt ccatattttg gaagaatcca
    24601 ctttctaaac ttgatttcag ttttcatgaa ggtggcttct cggacagtgg ttttatatgt
    24661 ttctagccac aagtcataaa cttctttaaa cgtttcgttg ttcgcttttt taataccgtt
    24721 tttatcgtag tccacttgca agcgtgacaa ggcaagctgg gcttcttttt ttgttttgta
    24781 acctcgtcta cgaaccttat caggtttgcc agtcattttg ttcattccaa gataggcttc
    24841 atagagccaa aatttacctt ttttagtttc atattgcttg aatgttgcca taatagtttt
    24901 ccctccatag tgtagcaagc cgtgggggcg gttggaggag gcagtttatt attattttga
    24961 atagtgttta tttagctcag taacaagagc tgtgaactct tttagaagat cattttcata
    25021 aaattcttga tactcttttt tctcatctgt atttttttct gaatcattaa ttaatgagtc
    25081 atatcgtaaa agcgtattta gaagagctgc gagataagca attggggtac tcccgtcagg
    25141 aaattcaatt cgagatcctg ttttaagata ttttattgcg tttgttaaat aaatcttctg
    25201 tgagacagtc aggttatctt cttcgatgtt attgatagca ttagaaagat tagaatcaat
    25261 tatagagtta tttttttcaa tagttttttt caattgcttt tcaagcacat ttcgcttaat
    25321 ttcgggcatc atatgcatat ctcttaatgt ataagatccg tttaatagat aggtagtgga
    25381 tacatctaat acctttgaaa gttgtttaag cctggtgtta ttgggaagat tatagccatt
    25441 ttcccaatta ctaacggcac ctttactggc cggtggggat aatttacctc caagttgttc
    25501 cattgagagt cccttttcaa ttctagccag tctaattctt tgtcctgtct ccactgcttc
    25561 ggggtatttt tttctagcca cattttcacc accattcata tattttctct actatatcac
    25621 gatttaaata tgtgtgaaac ataaaagttc aaaaaagtat gttaaaattc ttgcaaatga
    25681 aaaggtatga tggtatattg agtttataag aggtatagaa atgtattcaa gagcctcttg
    25741 cattttttta aaatctttgt atagaaatgt atgctaacga ggggggataa atatggaatt
    25801 aaaaattgac aaactcacgc ttgatgatcc agcttttaaa attattgacg atcgagttgt
    25861 tcaacaagta gagcaaactc aaggaacgtt actttcatta ttagaaaaaa agtaccagtt
    25921 tccaaaatat atgaacaagg cacaagctgc taagtatatg aacgtttcat acaatactat
    25981 gatgcagaaa tatgttccta acggtttaaa gttgattata gttgatggag tcgtaagaat
    26041 ttcacaagat gaatgtgatc gctttatgga agaataccaa aaataaacgc aagccgtggg
    26101 ggcaaaggga ttaataggag gaaataataa tggaatcgac acaaaataaa gcaatcgaaa
    26161 aagtattatc aacaattctt acagaagaca cagcggcaga attagcaaac ttggaaggca
    26221 aagcgctaga agaaacgttt gaatgtttgt acgaacaaat ggactatcaa aaattgctac
    26281 cacaagaacc aacggtaagc ggagtattgc gtggcttgaa cgatttagta caagcagagt
    26341 ttaaagaacg actttctatt gaagagtatc aagaaatttt gtatcagcaa gttgagttgt
    26401 tagcgagctt gctagggctt gaactggagg acgttgaatg aaaactgaaa tttggaacgg
    26461 acacattata agatttgtcg atattaatga tgaatggtgg gcagtggcga aagatgttgc
    26521 ggaagcgtta ggtcttaaac aggttactag agccattcac tctttgccta aagatggggt
    26581 tactactagt aaggtcatcg attcattggg cagaacacaa gatgtaaaca tcatcaacga
    26641 aaaaaaatat ttaccgcatg gcattcaaaa gccgtaaaaa agaagccgag gcatttcaag
    26701 actggatttt cgacatcatc aaagaactac gccagtccac aggacttgaa ggctttcaag
    26761 tattccgcat gctagacaaa gaacaccaaa aagaagcaat gactaaatta agccacgcta
    26821 ttactgaacc aaaaccagta gactacatta aagcgaatgt gatagccaat aaagctgtat
    26881 caactattta tggtcattcc aaaatggtaa aaaagaaaga tatgactcct gaaatgttgg
    26941 ttgatcgtga acctatttta gatgagacag tagaattaat gactgttaaa gaaaaatatg
    27001 ggttgcagtt ttcagtgagt gagaaaattt ataatcgttc tgctgaattg cagacaactt
    27061 aggagcatta acatgatcga gaacaaagaa aaagccttat ccataacagt cttggcggac
    27121 aggataaagg caacttaatt gggataaaac ccgatattct ttgttccgat tataccatat
    27181 taaaaaatcg gagggaacca ttatgaaacg aaacttaatt ataggaagca ttttaatcgg
    27241 cggaattttc ggcttattac cattaattgc aaaaatagct atctttgcag gattagcgtt
    27301 atatctcttc actgcttatg acgagtacga ctatcaatta agaactggag ggcataagta
    27361 gatgtacaaa acaaaactat taaaccaact cgatagcctt gaattagagg aaattaatca
    27421 agggatcgct gaactagaaa ataatatcgg gaaaacctac tttggaaatt cattcaatga
    27481 aaaactaaca gttttgtatg tactcaaaaa acatgcagaa cacaaaataa tttgtcgaga
    27541 aatcaacgag ctaaaaaatc agattttaac cgcatggtta aacattactg atatgcaaga
    27601 agcaagggtg aaaacattta atacatgggt aaagtatcag aatcaattaa aaggtgctga
    27661 atttgttcgg gatgggttga aatacgaact tgagcagttg aagctcatgg aggtgtctga
    27721 atgatttata ccagtatttt gaaaaaggag gagaggttta ttggcaaata attatcaagc
    27781 agcaacggaa ttacaaaagt cgggatggtc aatttatccc ttatctcctg gaactaaaat
    27841 tccaataaaa ggctctagtg gttggaatga tgcaactaac gattctaatc aaattaatga
    27901 atggtggacg gaagatgcaa ctagaaatat tgggttaatg cttgaagatc atcaaatggt
    27961 ggtagttgac ctagatcaac attctgaaac acgaaacggc gttgagaatt ataaaaaact
    28021 tgcttcaatg tatgatccat ttccttctac ttatacggaa atcacaccag gtaaaggaat
    28081 ccatttcttc tttaaaattc ctgatggtgt aattatccaa caagaaacag gtgcatttgc
    28141 tgatatcttc gggcgtgatg ataaaggtaa aagtttaagc gggattgata ttatcactaa
    28201 aggagttcca atagcgccaa ctatgattat ttcaagaagt gttgggagag catatgaacc
    28261 tattgaaaac gtatctctaa ataatattgc accagctccc gattggctag tgaacctact
    28321 gctaaaaaaa ccgcaagaaa taaagactca ataccgaccc gtagcaaaaa cagggacagc
    28381 aaaaaaatta gatatgattg ttcaaggcgc tggcgaaggt agtagaaacg atcatttaac
    28441 taagttatgc ggttggttac tatggcacgg tgtagataat caaactttag cagaattgat
    28501 ttatacagca aatgcgtaca atgaaccacc gctaaaagat aaagaagtga atcaaattat
    28561 tcgatcgatg attaaaaaag atttgagggg gaaagctcat ggctcataag cataaattag
    28621 atagtatttt agactttccc gaagccagcg agcgtgaaga caatatcatt gaattgaaaa
    28681 cctggatgtc acgattaaga tgcaataaag acgatcagat taaaagtaac agtgttgtga
    28741 atgcagaatt gattttaacc aatgatagca atttagctgg aacaattgct tataacgaat
    28801 ttagcggata cattcaccta ttaaaagatt ctccttggat caatcgtacc gctggagaat
    28861 gggaagacag ttttgaggat gccttaacgg catatattga agaaaactac aatgtagttt
    28921 ttgatgacaa caaaatacac aaagcagtag taaatgtggc tagaaagaat gttttcaacc
    28981 cagtaaaaga acgtatcgaa aaagttaaat gggatcaaca gcctagactg gaaacactgt
    29041 ttattgacct tttaggggta gacgacaacc tttacacacg agaagtaaca aaacgttgga
    29101 tagtcggatc agtcgcacgt atctataaac caggaataaa gtttgagatc gttccagttt
    29161 tggatggacc gcaagggatt ggtaagagta ccgttccagc attactttac acggatgatt
    29221 ttttcaccga ttcattagat tcgctaggtg aaaagaaaga tgattatatg cagcttcaag
    29281 gcaatgtaat tattgaactt ggagaattat cttcaatgaa caaaactaaa attgaacaga
    29341 taaaaaactt catcagtgcc aagatcgata aaattagacc tccttatggt cggaatgtca
    29401 tagcttgggc aaggcaatgt gtatttattg gaacgtcgaa tgatgggcag tatttgaaag
    29461 atgatacagg aaaccgccgt ttttatcctt tgccttgtaa gaataagcca aagatgaatc
    29521 cgtttaaaac aaaggatgaa tattttcttc aagtgttagc tgaagcaaag gtgctttttg
    29581 ataaaggaca acgaatctat tttagtcccg atgaagataa agaagtctta gaaattgcaa
    29641 aagactatca agaggatgcc aagatggaga atccaattaa agaagcaatc actaaatatt
    29701 tggaaatgga aatcccgtat aaatgggaag aagctccagc gtgggcgaga cgttcttatt
    29761 atcagaacta tcctgacagt aaatgtaatg agaaaaattt acagcatttc tataatggag
    29821 ctactatgca acaagagttt tatttagttg atagcgtact caccgctgat attttggaag
    29881 ctgtttttga taaacaagca aaggatttat tgaatggtcg atcagatgca gaaactaaaa
    29941 agattgcctt gattattagc agtattcctg gttgggaacg gaagcaactg ccaaaaagaa
    30001 ataaacgacg aggattttac aataagtcaa atgcctatga aaataaaaat cgagctggaa
    30061 agtacaaaaa gtaacgggta gtaacgggtc agtaacacct agcccgttac tagtttaccc
    30121 ttactgcccc aagggataca gcaatttggt aacggggtaa caccttattt ccttaaaact
    30181 tttgaaatag ttagctaata tgctggggaa taatgttact ggattttacc cgttacctgt
    30241 taccaaaacg ctagaaccct tgtccccgtt gggattgagt ggtaacgggt acctgttacg
    30301 taggtgttac cacgtgttac gaatcaagga ggaaagtaaa tgctcaatat tatttcaaca
    30361 aacaaagcac cgaactttca atatacagat gaaatggatc ggtttctaat gaacacgctg
    30421 gcatttagtg tgggattggt aacagaagat tattccacat ttgatccaga agtattaaag
    30481 ataatggaag aagaacctga ttggctgcag gagtcagtag cgtggtgtca atcgctagta
    30541 gtgggatcat tggtagatag tggcaactat gatgatacag gtgagctaat ggatgaattt
    30601 aattgtctgc ttaacctata tgatcgagcg agacaacgag agcttacatc gaatgaggat
    30661 aatttgtttt taaacatcca tgataagttc ttggctttgc tactaacaga tgatgaattg
    30721 ataactaatt tattggaggt agaataatta tgcatatgga aagtattaga tacttcgatg
    30781 gtgagaaaca cattgagtat tcaaacactc aagaagatgt agactttatt agttttgagg
    30841 aagataaaat cactaaagtt aattttaaag acggaacgtg cttgaagata gtttcgcctt
    30901 atattgaata caaatcaaaa tgggaacaag atgattctga cgaacgagat cgctttggtt
    30961 ggtaatctat ggcagttaaa aaacaatgta atcatgctgg atgtaaaaca ttgattgatt
    31021 ataggcagaa gtattgtggg aagcataaag caaagcaaac ggcaattaag cgtgaagaaa
    31081 gaaagcaaag cgaaggtaaa tattttcaat tctatcaaag tagaacatgg cgaaaggctt
    31141 catacttgta tcggttaaat catccagtat gtgaggattg cctagaagaa ggcttgataa
    31201 ggaaagctga tgttgtagat cacaaaattg aactaaaaga tgattggaat agacggttag
    31261 atgaaactaa ttttcgttct ttatgccatg cacaccataa ttccaagaca gccaatgaaa
    31321 gacaaagacg agaaaagagt accctttgag ttaagggggc atagtgtgaa acttactgac
    31381 aatcgatgcc tactcatttt ggtacaaata accgttgtga aaaggcataa gggtaattac
    31441 aaataacgat tacacttgta atcacaagtg aaaaagtata taatgaaagt aggagattta
    31501 ttgaaaaata caattgttat tactgataaa acagggaaac agcgcaccat tgagttacct
    31561 cattttggtc aagtgaccat tcaaatgcag aacggaaaaa tcatttatat agataaatta
    31621 gataaagaaa aattctgatc gaaaaacgaa ggaatgtgag cttaattgct tacgttcctt
    31681 tttcttttgt ctgaaaggag gacaacatgg gacaccctaa aatattacac gatacaaaag
    31741 gcaatatatc aagtgaagaa aaagcaatcc gtgtggacgc tagagaagaa ttgttcaagc
    31801 agcaaccatt aataaatatc acgccccctg actggatggc agcgagcgct agaagtgaat
    31861 ggaatcgtat cgtaccaaca ttaaaaaaag attatccatt gagtgaagca gattatggtt
    31921 cattggtagc ctattgttta gcctttgctc gaatgaaaac agccgaagcc gagataagaa
    31981 aatcaggtac gtttatcaca tgcgaaaacg gagtaaagaa agccaatcca gcggttcggg
    32041 ttcaatcaca agctatgagt gatttgaaaa aacaagccac ctcactaggt atgaccttag
    32101 aatcacgatc aaaactagct ttgaacaagg ttaaaaatga tgaacccgaa gacccgttca
    32161 aagagttgat gaattcatga atgattacat tgagaaagtc ctatcaggta agctgattgc
    32221 acccaagaaa attatccaag cgtgtgagcg tcatataagc gatttggagc gttctaagtc
    32281 agatagcttc ccttatgtgt tcgatgaaga acaagccacc aaagcgatta aattcattga
    32341 gttgctacca tctacggacg gtaaagcaat caagatgtta ggatttcaaa aattcattct
    32401 aggcagtctt tatggctggc gtactaaaga agggaattac aggcgattca atcgagcgtt
    32461 taccagtatg agccgtaaga atgggaaaac gtatatcgca agtggcatgg ctgccaatgc
    32521 gttgattatg gaacaagaac cagcagaagc aaggcaagta ttgtttgtaa gtaacgcttt
    32581 gaaacaagct aaattgggct atgatatgct gtctaattca cttagaaacg tggtcaagtc
    32641 tagtaagttt ttaagaccac aactgaaaat tatgaactct aagattcagc acttgccctc
    32701 taattcgttc gctatggcac tggctagtga aaccagcacg ctagatgggt ttgcaccaac
    32761 aaccgcaatt cttgacgagt ggcacgaagc aaaaactcgt aaaacgtaca acgtcattaa
    32821 gtcaggaatg acccaacaaa agaacggctt attgtgtgtt attagtaccg ctgggcttga
    32881 tttaaacgtt cctatgtacg aggaatactt attgttagat cgtgtgctaa aaggcgaaga
    32941 acaagccgac aggtacttta tagcgatatg ggaattggac gatcccgaag aaattcacga
    33001 tcaagagaaa tggatcaaag ccaatccgat ttttgaaagt gaagaaatca aaaaagtaat
    33061 gattccaacc attcaagatg atgtgaacct tgctttgaaa caaaataacc ttaattctgt
    33121 attggtgaaa aacttcaatt tatggagaca agcgagtgag gacagctata tgattgctga
    33181 agactggcag gcaaccgaag tagaaccaca agatattaca ggcaagcccg tttatatcgg
    33241 agtggattta tctaaaacag atgatttaac tagcgtttca tggatcgtac cgctagataa
    33301 cggcgaactt tattgtgatt cacatagctt tgtagccacc aaatatgggc ttcaagacaa
    33361 agaaaagcgc gacggtttgc cttatcgaga actagaaaaa gctggtgagt gttccattac
    33421 tcaattagaa agtggaatcg tggactatga ccaagtattt cagtttattc aagatttgat
    33481 tcaagaaaat gatttggaat gtatggggat ttgttatgac ccgtataacg ctaattcgct
    33541 tatcagtaaa gctgaaaaag ccaactaccc aatgttagaa gtaagacaag gaacgattac
    33601 tctaaacgtt ccgacccgaa cttttagaga acaagtttat gaaggcaacg ttattcacaa
    33661 taaaaatacg attctcaccc atgcagtgaa caacgctatt ttaaaaacgg ataacaacgg
    33721 cattcagatt aataaatcaa agaacagtaa caaaattgat ccaatagctg cattaatcaa
    33781 tgcctatgtg tttgcaatgg attacttcac cacaacggaa ggagcgaaag cagacaatga
    33841 attttataca agtgaagaat tttctttcta attacattca taccgttctt ttacttcttg
    33901 gattggtgtg tgtgttggtt gctatcacct tactaacaaa tgtctactat ggcttgttag
    33961 cgctgggcat agtgcttatt ggggtagcgg tcatgctaaa tacagaacag aagggaggtt
    34021 aaaagatggc attttttaaa gcgagacaaa atacaacggg agatcctttc ctacaacatg
    34081 tggtatcaat ccaatcggat gattacacca ccagctttac aagcgttcgt gcattaagaa
    34141 atagtgatgt gtttgcagcc gttcgaatta ttgctagtga tattgcttca agtccgattc
    34201 aattggtcaa aaacaatatg ccacaagctg ataatgaact ggtgaagtta ctaaacgaga
    34261 aacctaattc agaaatggat ggttggcatt taaagtttgc tttggcggtc aatatgttgc
    34321 taaacggtaa tagctttgca gagattaaac gcaacggtga aaaggtagaa gaaattcact
    34381 tattacctaa ctcaagtgta acggttactc aattagataa tggtacgttg tcttatcaga
    34441 ttggtgataa aaaaagacgt gtgaagtcta gcgatatttt gcacttcaaa tatttcaccc
    34501 aagatggttt aacaggatta ccaccacttt atgcgttacg tgatgaactg aaaattcaac
    34561 aagctggcaa ccgcacattg cacaatttct ttactcgtgg tgtcagtggg tcaggtattt
    34621 tgaaggttca taagtcagat ttagacggat cagcaaagaa cgcaattcgt gaaaagtttg
    34681 aagaagccaa cggttcaagt agtggagata atgctcttag aacgatcatt cttgatgaaa
    34741 caatggacta caaaacatta gaaataaaca ccgatgtttt gaagcttatc aattccaatg
    34801 attggaacac aaaacaaatt gccaaggcgt ttggtgtacc gattgagcgt ttaggcgttg
    34861 aaaatgaaca ttctagcacc gttcaaagta atctccagta tatccaaagt acgctgattc
    34921 attactttaa tgtgtttgtg agtgaattag atacgaaact tgaaaccaat atccgcttta
    34981 attccgatca gttactagag actgatcctg aaacaaaagt aaaaaatgta ttggatcagg
    35041 tcaaagggtc acttctcacg attaatgagg ggcggtcgaa aatggggcta ccccccatgg
    35101 atggtggcga tcgtttacta gcaagtttga actttacgta tttagatacg ttggagaaat
    35161 atcaattaaa agaacaggaa ggagttacac cagttgaata atgaagaaga aaaggaaaaa
    35221 cggctgacag aagaagctga gctaacagcc gattctccca aagtagagaa agaaaatgaa
    35281 aaacaaccaa cagacggcaa aactatttca ggctatgcgt tgaaattcgg gcaaccgtca
    35341 aaagatttag gcggctttgt agaagtgatt acacccgaag cattaaaaga ggtggattta
    35401 tcaaatgtgt tcttattata tggacacgat tattcaaagc cactagctag tgtcaaagca
    35461 ggtacgttga aattgaatgt cgatgataca ggattacatt ttgaagcaga gctaaccgac
    35521 acgacctatt caaatgatgt gtatgagaat atctcaaaag gggttgttga ttctatgtcc
    35581 tttgggttcg tgcttggatt agattcattc gacaaaaaag aagatggcac aattgaacga
    35641 tcaatagata aaatcaaagc acttaatgaa attagtgtcg tgaccgttcc cgcttatgat
    35701 tcatcaaatg tccaagtaaa taagcgttct tacgaatcgt ttatgagtaa caaccaagca
    35761 aaacaaacaa acaatagctt agaatccact tctaaagcac aaaaggagag taataacatg
    35821 gaaaaaactt taatcgataa cgaaaaaaca gaattgcgtg ggtatgaaga atatatccgt
    35881 tcacaaggcg aagtgcgtga tggagtcact actgtaaatg cagcggcagt tgttcccgaa
    35941 gaagtaatcg gtgaagtctt tgatttgaaa cgttcaaatt ataacttagc tcaatatgca
    36001 acagtaaaaa cagtatcaaa tggacaaggt aaatatccag tagcaactaa ccaacaagca
    36061 gtgttagcaa caaaagctga acttgctgaa attggtgata ttgacgctga aatgtttact
    36121 tcagttgatt ataaagtaga aactcgtgct ggtaagattg ccttatcaaa tgaggttgtg
    36181 gaagattcag cagtgaatat tgtacaagag gtcaaagatc agttagcgaa attggtagaa
    36241 aacactgaca ataagcatat catggattta ttaaaaacat tcactaagaa aacggctgct
    36301 acgttggacg atttgaaaca actatacaat gtggcattag acccagcatt aaataaaatg
    36361 gtaattctaa accaaagcgg atattaccac ctagatacat tgaaagattc agatggacgt
    36421 tacattttac aacccgatgt gacagcacct agtggtaaat cattattcgg tatgccagta
    36481 gtattgattg cagatacatt gtttgccaat cctaaagctg gaacattccc tatgattatg
    36541 ggggatattg cacaatctat ctttgttgct cgtagaaatc aagtaacgac tcaatgggaa
    36601 aaattcgatt actactcaca aggacttgca gtgatcgttc gcaacgatta taagaaaatt
    36661 gatcaaaatg cttcagtgta tattgagttt acgccagttg taacaccaaa agtatagaaa
    36721 aatattgggc ggcggtttat cccactgcct ttttttcatt aggagagata aaatggtaat
    36781 tttggatagc ataaaaaaaa gtatgcggat cgatcacact attgatgatg actttattca
    36841 acaattgatt gatacagcag gcgaatatat aaaaagtgct attgatagta gcgcaacgga
    36901 taaagatatg gataattatc agcaatttga tttggcggtg tcattactta ctcaacattg
    36961 gtatttgaac cgtcaagaag ccagcagcga acggatacca gtaacggtac aagcattagt
    37021 acaacaaatg agaggtgctt attatgccaa tcattaagaa tgtaaatgaa ttgactgaga
    37081 gagtcaagtt taaaaagaca aaacgggtaa aagatgaaga tgggcagatg gtggatagtg
    37141 aagaaactgt atttgaatgt tgggctaatg tgcgttcaca aatgctaaaa gacgtgcttg
    37201 ctagtgtagg taccattctt gaaggaacgt tgacgtttat tattcgatac gatcaagatt
    37261 atgaactaac aaacgatatg aaagtagctt ggaaaaacaa gagttataaa attatttcaa
    37321 tcaatgaagg aacggcgttt aaagattata caacgatcat agctaaacag atttcttaga
    37381 atgattacac ttgtaaacgt tgtgggtatt tagtataatt aaggtagtaa atgaagggtt
    37441 agctgcctga attttgcaag acttagctag tcgaaatcta ttgttggact gaaaattgta
    37501 ggtgtggttg caaacatgct ggactaagaa agatgataga tgatgaacgt tcaattttaa
    37561 tacaggcatg ctaggactga ttggatggtt ttggcaagct gattgtgtag aaatcaaaga
    37621 acacgtctgt tgtggacgtg ttttttttgt tataattcaa aagaggtgat ttaatggaca
    37681 aagcagagtt tacaaatttt tattggaagt attatctaca tttagaaaat gagtttatta
    37741 ataccacaga ttttgtgatg ctcgatgaga ataatttcaa aacattctct attgaatatc
    37801 aaaaactact actagcgatt ggttcggagt gtgagattat tttcaaagaa ctttgcggat
    37861 ttgatagcag tagcaataaa agcataactg attttaaagc tgttattaaa tctagcgaat
    37921 taatgtgttt ggataatggt gttagggtta tgaattcttt aacattgact tcactaaaac
    37981 cttttggaga tgggtggcct gaagatacac cagattggtg gaaaatatat aacagagtaa
    38041 aacatggtcg ttcttcaaat tataaggaag caaatttgga aaatgtgttg tatgctttag
    38101 caagtctata tttattagag gaatatttgc ataagaaagt aatactagaa ggtgaagtag
    38161 actttataag tccagagtca aatctattta cgttatcttg ggaaagaaaa cattcgtcga
    38221 tgcaaaaagt gacgtttgaa aaagtggatt caaattatgt agctccactc attgatttta
    38281 aagaacaaga agaaatttaa aaacccccac ggcttattga agtttttgta tgccatattg
    38341 tatgccattt ggtttaaatc gcttgaaaaa tagtgataga taactctaat tcatttagtc
    38401 ataaacgctg ttttgataac ctttatagtt ctttgatatg ggtttctcat tcatggcgga
    38461 agaagaagaa tagaacaaaa gaatgaatta ataagatata aaaaaaggga ttgtgacaaa
    38521 cgtcttgtca caatcccttt ttccaaataa acgatgttca atcagctgcc cctcggcatt
    38581 cgctcgaaaa acggcgaaat atgaagaact attttgccat ttgtttctct aataatcagg
    38641 tcaaaacgtt gacttcacag tctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc
    38701 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
    38761 ggagctgaac ggcaggctca cgacctcttt cttaaacggt gttcaaaagc ttgcatctgc
    38821 ggtaataagt tcaaccaaac gaaaattatg aggagctatt tttgtttggc tgttcttatt
    38881 acttgatgca ggacagcttt ttcacaacct cttttcgttt agcactaaat ttccttaaaa
    38941 agtagaaaag ctttcgaagc ctcgtttgtt tcttttactg tataataaaa gaaaagagta
    39001 acaatcgcta aaagatataa acaagagaaa ggaaatacaa gatgattggt ctgatttatt
    39061 ttgtggtgat cgttctggcg aacacagttg gcgctatttc cgggatgggt ggaggcgtgt
    39121 tgatcaaacc gattcttgat cttatcggcg cccattctgt tgcagggatt tctttttact
    39181 caacagttgc ggtattcacg atgtccattg tttcgactgt gcgacaagta tcaagcggga
    39241 aatcactgaa ttggcagatt gtcggttggg tatctagtgg agctgttgtt ggaggagtag
    39301 tcggcaacat tgtattcgaa gtttttttac agttatttga aaacgagaag catgtgcagc
    39361 tgatccaaat ttttttgacg gtattgacct tggtttttgc gttcttctac accaagcatc
    39421 atcagccaaa atttcatttg acgtcatgga cttggtacct tatatgtggg gggggttctg
    39481 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
    39541 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
    39601 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
    39661 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
    39721 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
    39781 cttttggtca attgttacaa tgcctttgtc atcttacaaa cattaggaga atctagacta
    39841 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaagagta tactatgata
    39901 aaaaaattca ttttaaaaaa tcagaaattg gctggtgatt tggatgttac gggaaatgcg
    39961 tttagaagat gcagaagcac tacgtgaaat caatgcggat caattagggt atgatatacc
    40021 attaaccgtt acggtgagac aaatgaagaa actgctgaag gagccagaga aaaatttttt
    40081 cttggtttat gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta
    40141 tgaatcgatt tttagcgatc caatgttcaa tattatggca cttgctgtgt ctaaatatgc
    40201 tgaaaaaaga ggcatcggaa aggctttgat gactggcgta gaagaagaag cgaaaaaacg
    40261 aggaataacg acaataaggc ttaattctgc agaatacaga gtagaagcac accaatttta
    40321 tgaacacatc ggttatcatt ccgataaaat gcaaaaaaga tttttgaaaa tcctgtaaat
    40381 ctttatcaaa aaaatacccg atcaccaagc cgatacttga tgatcaggta tttttgtttt
    40441 tttgactaag aaagtaattg catcaagaaa ccagagatga tcaaaacgat ggcaccacca
    40501 agtcgattgc ccatttgagc aaaagcaata agttccatcc gattagctgc agataagacg
    40561 gctacatttc ctgttccgcc catcgagttg ttgcataagc cagcagtaat agttgattca
    40621 attggatata aaccaaacaa acgaccgaca aatcctgaaa caactgaaat cgcgataaca
    40681 cttgtcagac ataagacaac aaattgccat gtcaaagcag aagctaatac attcagattc
    40741 agcaacgcaa taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagtta
    40801 ttgaacatga tagctgcatc ttcataatac tctggtaata ggttggcgat tttacagatg
    40861 acaacgataa taatcataaa tgcgtaagca tggactttag gaacaaaata gttaccaatt
    40921 tgtcctaaga tgaagaaagt caatgatacc atcaaaccaa cacctaactg tggaatggaa
    40981 ggtttcacag ttcgaggttt tccatcgcca agatcgcctt tttccattaa tttaccattg
    41041 ccgttgaatt ttggcattga ttgtccagca cgagcaacta atgctgcacc gatgatagct
    41101 aagacatttg tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcatccgta
    41161 cctaaaacat ttgcataaat cgttgataaa ggaacggctc cggctccaac accaccagcc
    41221 atcgcaggta atgagatgta taaaacggaa tttgcaaaac catttccgat aagcatgccc
    41281 acgagtccta cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga
    41341 acagaagctt tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca
    41401 gcaatataaa agtcccaaaa gcccatattg ctgacaaagt tttctgtgga tttcactaca
    41461 tattcaggta agatcccaaa tgtagcaatc gcagcagaag cgaagatcgc aaaaacggaa
    41521 ccaccaccca aataactttt gacgataggg atttttgtcc cgataaaatg aaataaatta
    41581 ccgaaaataa ccaaaactgc gattggtcca atcatattcg tcggtaattt ccctaaagcc
    41641 attgcaatga tcaggacaag gaccatgatc aaataaagtg gcaaacttac gccggtgatt
    41701 tttgtggacc aaaaaccttc ttttttcttt tcctcaactg cagttgcagt tgttgttttt
    41761 tgtgccattt ttacagatcc tttcttattc tgtatcatat gaaaaaagcc aaagaaaagc
    41821 gacaaaagag caggagttgg ctggccgctg ttttgccgca gtcgcttttt tctttttaga
    41881 cgtcatacaa agtttctgta tgttctttta ctataacgat cttaatcttc taaagattta
    41941 tattcaggaa cccatttagc agattcaact gcttctttga tatccgtgat ttcttcacga
    42001 ttcaagtttt gtgcgacaac tgattttgcg acagtttcag caatcgtttg agaaaattca
    42061 gtgatttttg ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg
    42121 cctaatgctc gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca
    42181 atcaaaccaa gtcctaaacc tgggtacatc aaagcattat ttgcttgacc gatttggtaa
    42241 gttacaccat tatattcaac atcagcagca gggatacctg ttccaactaa tgcttttccg
    42301 tccgtccatt ctattaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa
    42361 ggcatgatga tcggacgtgg tgtatgagca gccatttctt tcacgatttc ttcagtgaat
    42421 gctccaggtt gggtagaagt accaatcatg attgtcggat ggatcgcttt tacaactgct
    42481 tctaagtttg tcaattcatc agcattagtg aaatctgagc gtttgcgagc gaatgggatt
    42541 tgaccaggag tcaatccttc tgtatcttca aacaataaac cttgtttatc tacttgatag
    42601 aaatgttggc gtgcttcttc ttcaggtaca ccttgacgga tcatctcatc taaaaggatg
    42661 tttgcgatac ccacaccagc agtaccagca ccaaatgtca aaattatttg atctttcaat
    42721 tgttcaccgg atatattcaa tccgcctaat acacctgcta ggacaacaat tcctgtacct
    42781 tggatatcgt cgttgaaagt agtgatttga tcttcgtatt tttctaagat cgctgcagca
    42841 tttcctcgtc caaaatcttc ccaatgcaat aacaattctg ggaataattc ggtagctgat
    42901 tgaacgaatt gatcgatgaa ttcatagtaa gtttcgcctt ctacacgagc atgacgattg
    42961 cctaagtata agggattatt caataattct tcattattag taccagcgtc gatcgataca
    43021 ggcaaaactt gtgctggatt aataccagca gcagcagtat agaccatcaa tttgccgata
    43081 gcgatgtcaa caccgttcac accccagtcg cccataccta agattccttc tgcatcagtc
    43141 acaacaatca aacgaatgtc tcgtccatct gccgcgttct tcaaacttgc tttgatgttt
    43201 tctggttcgt cgattgaaag aaatgcagca tcttgcggtt tcaagaaaat ttcgttgtat
    43261 tgttcgattg aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt
    43321 tcgcccatca atttataaaa caatgtccga ttcgtattga atagattcat taagaagata
    43381 cgtttttcca aatctgtttg tttgcttaag tattgaccat aagcttgaac agattgttgt
    43441 tccagtgttt ggacagtact tggaagcata ccagtgattc catattttgc ccgttcttct
    43501 tttgtaaaag ctgttccttt atttagaaaa ggattattta ataagtttaa ccctgtaagc
    43561 atgaatgatt cctcctaagt tttaatacaa acgaagtata gaagtcagat gatgatatag
    43621 tcaaaaagag caggtatgat aaattttatt tatatgtaat atagccaaaa gaaatcaaaa
    43681 tagcagtatt tgttcttttt atatcgacaa tccttgcttg aatactatgc gtatctctta
    43741 tatgctagag aaagaaaagt agaagaataa gggatagatg gaatggataa ataggatttc
    43801 tgaggaaaaa agcacatgat agcgttgata ggagtcacct atgtcaggtg ataaaaatac
    43861 ataagtagaa aattaagagg aggaagagaa aatgaaagaa gttcaattga ttagaaaaag
    43921 cgaactatca gaaggcggtt gcaatgcatg tggcgtagta gaagcgacaa gctatacatt
    43981 aaaattagat tccaatcaag caataatctc tgaactgaca gtaggcggtc ttgtggacag
    44041 tctggcgctt gctgaaggtt ttactggaga agatatttat gagatgtttt cagaagtccg
    44101 tcagttaaaa aaaggagaaa aatgcataga ggttcatcat gagagcccga atgtgaggtt
    44161 caaacgtggt gataatgaga tgatcttcaa gaatcatgtg tctgatcaca ctgaactata
    44221 tgaaatcgtg aatcaaatac tgtctgggat ttttgaatta ggaccttatg agttcaagga
    44281 agaaaacggt aatccaaaat taaatgaaga atggcaagaa acgattgaaa cacaaagaaa
    44341 caatccacac ttatttcaat agtcttccac agtatttcgt aaaaatgttg atgaaaaaga
    44401 aacaccgtga atcagaggtc gtgaaaaacc tgccctgcat caagtaataa gaacagccaa
    44461 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagtttt
    44521 tgaacaccgt ttaaggaaga gactgtgaag tcaacgttct gacctgagta ttagagagac
    44581 aaatggcaaa atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg
    44641 attgaacacc gttttatcga gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
    44701 aattttgtga aaatggcttt ccacattttt gcataaattt gacttaatac cgagaaaatg
    44761 gcttttgaac accgtttatt caaaaaaagg gactttgaca ctacttttgt caaagtccca
    44821 tcggtttttt atctattttt attacaatca tttcttccag agtagtgaca tctggaatgg
    44881 ctgaaatgta aacagattat ttgcctgtat caatgtcgaa gagtaatata aaaagtgtaa
    44941 acgctatcat attgttcatc attcaaattt tatagagaga aggcattgag atggaaaaaa
    45001 catatcaagc aggtacaaat gaaggaatag tagattttat caatatggaa gatttagaaa
    45061 tcgcggcttc acaagtgatt ccagctggtg gttatggtta catcagcagt ggtgctggcg
    45121 acttatttac ttatcaagaa aatgaacggg cattcaatca tcgtttgatt attcctcatg
    45181 tattaagaga tgtagaatta cccgatacaa ctacacattt tgacgaagaa atgctgactg
    45241 caccaatcat catggcacca gttgctgctc atggattagc tcatgtgaag gcggagaaag
    45301 cttctgcaaa aggtgtagca gattttggga cgatctatac agcaagttct tacgcttctt
    45361 gtactttaga agagataaga gaagcaggcg gggagaaagc accgcaatgg tttcaatttt
    45421 atatgagcaa agataatgga atcaatttgg atattcttga agtggccaag cgtaatggag
    45481 caaaagcaat cgtattgaca gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca
    45541 gaaacgggtt taccttcccg ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag
    45601 gccaaacgat ggatgctgta tataaatctt ccaaacaaaa acttagtcca aaagatgtag
    45661 aatttatcgc tgctcattct gatttgccag tctacgtgaa aggtgttcaa tcagaagaag
    45721 atgtctatcg atcattagaa tctggtgctg gtggaatttg ggtatctaac catggtggcc
    45781 gtcaattaga tggggggcct gctgcgtttg attcgttgca atatgtggca gaagcagtag
    45841 ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg tggacaacat gttttcaaag
    45901 caatcgcatc aggtgccgat cttgttgcta ttggcagacc cgttatttat ggattgtctt
    45961 taggaggaag tacaggagtc catcaagtgt ttgatttctt taaaacagaa ttggaaatgg
    46021 tcatgcaatt agctggaaca caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga
    46081 atcgttttat ttaagaaata aatgacgaaa aaaataggag tgcggcagat gatgccgtac
    46141 tcctattttt atagttcaag gatgtcacta atagaataat ttgaaacgaa aaaataaaat
    46201 tgaaatattt tttcatactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    46261 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    46321 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    46381 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    46441 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    46501 aataccgttg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    46561 gaaaatccag caactggcca gacaggttct ttaacattgg atgtagttcc tgaattgcca
    46621 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    46681 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    46741 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    46801 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    46861 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    46921 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    46981 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    47041 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    47101 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    47161 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    47221 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    47281 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    47341 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    47401 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    47461 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    47521 cacatgcaga aacaactata cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    47581 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    47641 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    47701 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    47761 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    47821 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgaatttt
    47881 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    47941 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    48001 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    48061 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    48121 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    48181 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    48241 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    48301 tatgatctaa gtccagttcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    48361 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    48421 gaaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    48481 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    48541 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    48601 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    48661 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    48721 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    48781 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    48841 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    48901 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    48961 tgccggtgcc cccatcaagg ttactagcgg tagtttatta tcagcaggaa gccatacctt
    49021 gcgttttggg atggggtcgt gggatacaaa tactctattg gcagatatta ttgctattat
    49081 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    49141 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    49201 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    49261 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    49321 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    49381 agagataaat acgtggaata gttacatctc aacaagtgat tcaacaggaa aatataatac
    49441 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    49501 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    49561 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    49621 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    49681 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    49741 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    49801 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    49861 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    49921 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    49981 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    50041 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    50101 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    50161 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    50221 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    50281 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    50341 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    50401 cattcatctt acttacaact atcaattaaa tactgtagat acttctgtac aatccgtcag
    50461 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    50521 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    50581 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    50641 aagttattat gaagcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    50701 cacgtctcct tggcaactga cagggacatt gacttcttta tttaagaatg atcaaggtca
    50761 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaaacaat tgattcagca
    50821 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    50881 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    50941 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    51001 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    51061 aacttctgct gttaaaatca gaaatgcttg gaggagtgga cttggaactt ttcagtttgc
    51121 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    51181 agatttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    51241 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    51301 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    51361 gctacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    51421 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    51481 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    51541 ccggtccaga aagtcaaatt tgttattttt atttccaatt attttggttt ggacgtcctt
    51601 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    51661 aaaaagagtt tagttttact attacagaag aaggacgatt gaaatttgaa atatggcttt
    51721 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    51781 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    51841 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    51901 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    51961 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    52021 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    52081 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    52141 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    52201 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    52261 ttgaattatt tgctcgctat gcatctattt tgcggacgct gatcaagcag ttagaattac
    52321 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    52381 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    52441 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    52501 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    52561 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    52621 cattggtgct tcccatgcag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    52681 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    52741 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    52801 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    52861 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    52921 taaattaatc gtagcaacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    52981 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    53041 agcaaaagaa gtagtgatct tcggtgctgg ttatattgga ttggaattag cagatgcttt
    53101 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt tatcaagata
    53161 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaatcaattt
    53221 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    53281 acttctatct caagcaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    53341 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    53401 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    53461 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    53521 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    53581 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    53641 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    53701 tgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    53761 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    53821 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    53881 acacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    53941 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta ttgtgtgggg
    54001 atataataaa tattaatcaa tcgagaataa aaaattcata taatgaaata gcggaagaat
    54061 tttttgagca taatattcgc caaaaattta tcaagtgata aaattgttgt ataaaatgat
    54121 tatttcagga ggaggagtta aatgttttta gcattaaatg aaattatgca ttcaaagtta
    54181 cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct acctggtatt ttttttgact
    54241 gggttagctt atggcttagc tcaagataat cgaacagcag tcgacaagtg ggaggcagat
    54301 tcgattgtgc tttcgaaaga tgcaaattca aatctaggga tgtccatgat tactaagaag
    54361 atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg cccagacacc tggagtcgtg
    54421 actagtaaag acagtacaga agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa
    54481 aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt ttgataacga tgatgaggca
    54541 gtaggcgata ttagtttgaa agaagaatat ggtctagccg taggagatac agttaagtta
    54601 tctggtagtg acaagacgtt taaactaact ggctttacgg atcatgccaa attcaatgtc
    54661 tcaccagttc tctatacaac catcaatgct taccaagaga tccgcttcga aaaagaagat
    54721 acaagtgaaa atgcacggat taacgcaata gttgtcagag gtaaaatcag taatttacct
    54781 gaagatttag aacagatcaa aatttctaaa ttcatcaatg aattacctgg atataatgcg
    54841 caagtattga cttttggatt tatgatcgga tttttgatcg ttattgcggc tattgtgatc
    54901 gggattttca tttatgtact gacgatgcaa aagattaata tctttggtgt aatgaaagca
    54961 caagggatca caggaggatt tattgcgcgt tctgtagtgg cacaaacatt cattttatca
    55021 tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt tctacctgat
    55081 gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt gatgctggta
    55141 gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga tccgttgaag
    55201 gcaattggat aagaaggagg aagagcatga aagcaataga atttaaagaa gtagaaaaaa
    55261 gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg agtatcgata
    55321 aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact tttttgactt
    55381 tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat caagaattca
    55441 gtaatcaaaa agaaagtgaa cgcgttaaac tacgttttaa agaaattggc tttattttgc
    55501 aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta gtagataaga
    55561 tcaaacatga acatcgtcaa aaagaagcaa aagaattatt tgagcagctt ggcgtggata
    55621 agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg gtcgcaatcg
    55681 ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact gctagtttgg
    55741 attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata
    55801 aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat agaatattta
    55861 tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc
    55921 catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga cttgtttatg
    55981 tccaagggat atctcggcac ttcaacaaga gagatcgcac aaaaagttgg gatcacccaa
    56041 cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt tttagaaaac
    56101 cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga aacgggattt
    56161 caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt cgatttgttt
    56221 ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca tcttttcttt
    56281 ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg
    56341 ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt gttagcacct
    56401 tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca attgatcgat
    56461 ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag aaaaatagtt
    56521 aggtgaagaa gcaagatatg acgaagatcg ttatatcttg ttttttttat ataggtagtt
    56581 tttcataaat acaaatttgt tattctctca tgattatcag ggtatatttt tatttattat
    56641 gtcggtgttc caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga
    56701 aggaaactat ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata
    56761 tgccaaaaat gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta
    56821 cagccccttt tttgcgtact taagctgatt ttaagttttg acccttagtc tggatctacg
    56881 aggtgagagt caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt
    56941 attatctgtt ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc
    57001 agtacgaaca gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca
    57061 ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca
    57121 cttgcaacta taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat
    57181 cagatcaaga caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc
    57241 acaaagaata tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct
    57301 gggcatggcg gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat
    57361 ataacattaa gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt
    57421 tatttgaccc atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa
    57481 gaaaaaaagg cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa
    57541 gcaactggaa tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt
    57601 gctgactcct tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa
    57661 gttcttcgag aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat
    57721 agtgatttaa aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca
    57781 caagcattga ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc
    57841 atatttttcg tttggccggg cttattatcg cagatgcaag cttttgaaca ccgtttaagg
    57901 aagaggttgt gagcctgccg tttaactccg cgtcacaagg agcaatttca aaaaacagct
    57961 tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta
    58021 ttcggaacaa gggactgtga catgactttt gtcacagttc cattattttt ttagcagctt
    58081 caaagctttt tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc
    58141 gcctttctcc aatagttttg ccgctacttg gtcagatttc gttccagctc ctgatggaag
    58201 ggtgattcca gcttcagctg atgctttgtg taactcttcc aaaaagctgg cacgactgat
    58261 gatagaagca gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt
    58321 ttcttgtaat tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa
    58381 ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat
    58441 cgcttgattg tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt
    58501 gtatttttct ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc
    58561 aagctggatg atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa
    58621 ttctgcaagc tgatcacgtt tcacataagc agcacatact gttagcggac caaaatagct
    58681 gccatttccg acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga
    58741 agaggctgtt tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc
    58801 tattgctgca ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt
    58861 cccgtctttt tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg
    58921 atagtatgct ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat
    58981 aaaattctcc tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta
    59041 agagattgcg agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa
    59101 aatatttgtg gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc
    59161 aaataggagg aaatgattcg tatggcgcac gaaaaaacac gctacaaagc agtgattgcc
    59221 aaccaaacat atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag
    59281 ctgatcaatg aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa
    59341 gcagcaatct tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaatc
    59401 ttggagctgg aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt
    59461 gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa
    59521 aatggtcaag cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag
    59581 aaacgaaaag gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga
    59641 aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt
    59701 ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc
    59761 gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt
    59821 catttggaat tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt
    59881 gatcaggtat ttgcttttca cctagatgaa gctttttatg caggaactgc ttttctgatt
    59941 attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg
    60001 tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg
    60061 gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt agtgtggtcc
    60121 aaaatcaatt cggcgagcat agtttcgctc gttttatcgt tgagagaacg ccatttctta
    60181 ccaacaagat gtatgagctt tgggtaacga atattttgaa ttaaggaaaa tagtgctgga
    60241 aaatatttcg ttttttgtac agagtagttt atttaagaag gctgcgtagc gtatgtccgg
    60301 cacagctctt ttttcatagt gaaagataag gtgataaaat gaataaccga atccttgaaa
    60361 cattggaatt tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact gcacaaggaa
    60421 aagaagaact ggcagaattg gtaccagtga gtgagaaaca aacgatcaca aactggcttc
    60481 aagaaactga ggatggattg aaagtgcaac gtctgcgagg cgggatccct atacctaaac
    60541 ttgaaaatat ccgtccccat atgaaacgga tcgagatcgg agcagacttg aatggagtgg
    60601 agcttgcaca agtttccaga gtattgtcta cgacaagcga attgaatcgt ttcattgatg
    60661 atctttctga cagtgaaatc gaatttgcac gtttgtatat gtgggcagat cagttagtca
    60721 cgatccctgt tttgagccgc cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg
    60781 atgacgcttc tcctgagtta aagagcataa gacagaacat tcggcgaagt gaacaagctg
    60841 ttcgtgaaca gttagatggg atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga
    60901 tcatcaccat gcgaaatgac cgctacgtta tcccagtcaa acaagaatac cgcggtgtat
    60961 ttggcggtgt agtccacgat caaagtgctt caggacagac tttatttatt gaaccaaaac
    61021 aagtggtgga tttgaataat cgtttgcgtc aataccaaat cgctgaacga aatgagattc
    61081 aacgtatttt aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa atcatccaca
    61141 atgcttatgt gattggtaaa atggatctga tgaatgcaaa agcacgattt ggtaaagaag
    61201 taaaagccat cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa caagcaagac
    61261 atccactgat cgatcaggaa aaagtagttt ctaatgacat cacaatcgga aaagactatc
    61321 aagccatcgt cattacagga cctaacacag gtgggaaaac gatcacgttg aagactttag
    61381 gtctgttaca actgatgggg caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa
    61441 tcggtatttt tgaggaagta tttgcggata taggagacga acagtcgatt gagcaaagtc
    61501 tatcgacctt ttcttctcat atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa
    61561 aaagtctggt attatttgac gagctcggtg caggaacaga tccgcaagaa ggtgctgctc
    61621 tagcaatcgc aatcttagat gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc
    61681 attatcctga attgaaagtt tatggataca atcgggcaaa tacgattaat gccagcatgg
    61741 agttcgatgt ggatacacta agtccaacgt atcgtttgct gattggcgtt cctggacgaa
    61801 gcaatgcgtt tgaaatctct agccgattag gtcttgatac agaagttatt gatgaagcga
    61861 agcagttgat gaatgatgag agccaagatt taaacgaaat gatcacagat ttagagaatc
    61921 gccggaaaat ggcagagacc gaatatctgg aaatgcgtca ttttgtttct gaggcacaag
    61981 aacttcacga tgatttgaaa gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa
    62041 tggaaaaagc aaagaaaaaa gcaaacgaag tcgtgtcaga ggcggaagaa aaagcagaaa
    62101 aaatcattgc tgatattcgt aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc
    62161 atcagttgat cgatgcaaag acccaattag caaatctaca tcaagaagaa actttgaaga
    62221 aaaataaagt attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt
    62281 tggttacgac ttacggccaa agagggacgc ttttacgtaa aaacgggaat cagtggcaag
    62341 tagaaatcgg tatattaaaa atgaatgtgt ccgaagacga gctgacgcct gttgctcctc
    62401 aaaaagagcc gacacaacga gttatccatg ctgtacgatc tgaatctagt agtcacgtgc
    62461 cgaatcaatt ggaccttcga ggaaaacgat acgaagaagc gctaagtgag gtagatcaat
    62521 atttggattc tgccattcta gctggttatc ctcaagtgac tattgtccac ggaaaaggaa
    62581 caggcgcact tcgcaaggga attactgatt atcttaaaaa ccatcggagc gtcaaaagct
    62641 ttgagtttgc accggcaaac caaggcggaa atggtgcgac aattgttaaa tttaagtaaa
    62701 gcttactaaa ggtaagcaga tggttcgcta tccaagtata gaaatgctat actaatcata
    62761 gattttaaaa atggaggtct ttgatatgtc acaagtaatt acagataaga catttaatga
    62821 agaaacagat aaaggacttg tattgattga tttttgggca acttggtgtg gtccatgtcg
    62881 tatgcaagca ccgattttgg atcagttgga acaagaatat gatgaagaag aattcagaat
    62941 tgctaaaatg gatgtggatg aaaatccaga aactcctcaa caattcggta tcatgagcat
    63001 tccaacatta atgttgaaaa aagatggtca agtagtagaa aaagctgttg gtgttcattc
    63061 aaaagaacaa ctacgtcaaa tgatcgatca atatttataa aaacttacag caaaaataag
    63121 ctgagggtgg gactgaatcc agttccgtcc tcggtttttt tgcggttgag ttaaggttat
    63181 tcaaattaat attttttgag tataattacg ttgtcaggct ggattgtttt tttgacagac
    63241 atgctataat aactttaatt gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa
    63301 acaaattagc tttacttcct gatcagcccg gctgttactt gatgaaagat aaaaatggaa
    63361 cgatcattta tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca tattttactg
    63421 gcagccataa tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt gaatacatcg
    63481 ttacagaatc gaatatcgaa gcattgctct tagagatcaa tctgataaag aaaaatgatc
    63541 ctaaatacaa cattatgtta aaggatgata aaacctatcc tttccttaag ataacgaatg
    63601 aaaaatatcc ccgtttggtg attactcgta aagtactaaa agacaaagca ctgtattttg
    63661 gaccatatcc cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat cgattatttc
    63721 ctctgagaaa atgcaaacct agtcagacga aggagccttg tctttactat catttgggac
    63781 aatgtctgtg tccttattat ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg
    63841 aagtgaaaaa gtttttaaac ggaggacacg aaaaaatcga gtcagagatc catcagaaaa
    63901 tggaaaaagc tgctgaaaac atggagtttg aaaaagctgc agaataccga gaccagatcc
    63961 gtgccattga gacaatcatg actcgccaaa aaatgacaaa tactgattta ctggatcgag
    64021 atgtatttgg ttatgcgatt gacaaaggat ggatgtgtgt ccaagtgttt ttcgtaagac
    64081 aaggaaagct aattgaacga gatgtttcaa tgtttccttt ctataacgaa gcagaagaag
    64141 atttcttgac ttacctcggt cagttttatc aggaaaatga gcatttcatt cctaaagaag
    64201 tcttgattcc tgatacgatc gacaagccca gtgtagaagc tttgttagct acgaaagtgc
    64261 tgcaaccaaa acgaggagaa aagaaaaaat tagttgaatt ggcgaataag aatgcctcag
    64321 ttgctttgaa tgaacggttt gatctgattg ccagaaaaca ggaacggacg atcggagcag
    64381 ttgaacgttt aggaaacgcg atgaatatcc cgaccccaat acgtatcgag tcttttgata
    64441 actctaatat catggggacg aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac
    64501 cggcaagaaa agaatatcgg aaatataaaa taaaaacagt caacggaccg gatgattatg
    64561 catcaatgag agaagtcatc tatcgcagat attcacgagt gattcgcgaa gggttgcctt
    64621 tacctgattt gatcttgatc gatggaggaa aaggacaagt agatgtggca aaagatgtct
    64681 tagaaaatca attaggaatc gatattccta ttgctggcat ggcaaaaaac gataaacata
    64741 aaacaaatga gttgctattc ggtatagatt tggctgtagt accgcttgag cgaaattcac
    64801 cagagttttt cttactccag cgaatccaag atgaagtcca tcgattcgca atcactttcc
    64861 atcggcagct acgcagtaaa aacagttttg cttcccgatt agatgaaatt gaaaggttag
    64921 gaccaaaacg aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat atcacagcag
    64981 ccacgacaga agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg
    65041 ataaattaca tcaggaatga ggaaagggca ctttacaaag aaagtaaagt gtcctatcat
    65101 tttatgtaca acaaaaagag cgtttacgtt ttgttggtaa ataataaatc gcttacgtct
    65161 ggtgtctaag gatatatgtg atttcttgaa gaaggagtga agaaatgtta gaagtcaagg
    65221 atcttgttaa aacttttggg aactacacag ctgtcgatca tgtctctttc caaatccccg
    65281 atggaaaaat catggggcta attggccaaa acggggctgg gaagacaacg acttttcgtt
    65341 tgatcttgga ttttttgaca caagaccaag gagaagtgtt atggaatggc catcatcttg
    65401 gggagaaaga ttatgatgtc atcggttatc ttccggagga acggggactt tatccgaaag
    65461 taacgattca agaccaactg ctttattttg cagaacttag agggaaatca agaaaagaga
    65521 ttgaaccaaa aatcgatttt tggatggaaa aatttcaggt aaaagggaaa aagacggata
    65581 aagtaaaatc tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc
    65641 atgaacctaa attgatcatt ttggatgagc cattcagcgg gttagatccg gtaaatgctg
    65701 aattgctaaa agatggaata ttggaattga aaaaacatgg ttcatgtgtc attttttcta
    65761 gccacaatat ggataatgta gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa
    65821 aaatggtttt gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt acgaagttat
    65881 tcttggaatc tggtttaagc caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga
    65941 tccttcatga agacaatacg ttagagatta ccttagaaga tcctgaagct gggaaagaaa
    66001 tctttactcg tgcgactcag ttcggctaca ttcctatgtt taaccagcaa ccgccaactt
    66061 tggaagagat tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg gattattacc
    66121 aaagatgtct atttaaaaaa tgttaagtcg atttctttct tgatcatgat attagtccct
    66181 tttatcatgc tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc
    66241 aataaaatcg gcgtattggc tgataatgag caaatcagtc agcaaatgag ccaagtcaaa
    66301 acagatgatt ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa agagctttcg
    66361 gaagaaaaaa tcgatgcgta tttagtagtc tcaactgaga atgataagat aaatgggatg
    66421 ctttatagcg aaaattcatt aggacaaagt acacaactac tgatacaaca acaactgact
    66481 ggcttgcaat cgatgcttcg agcaagtcaa ttaggcattt caccagaaga agtagctagc
    66541 ttgagccaac aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg
    66601 acagttggag aagataactc gacagtccaa tatgtcgtaa gttatgtagc aacgattgtc
    66661 ttgttcatta ttattttgac gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa
    66721 ggcacgcgga tcatggaagt gatcctgtcc agtacgactg cacaaaagca tttttacgga
    66781 aaactaacag gtgttttact tgttgcatta acgcaaatgg cattatacgg tattattttt
    66841 ggtataggat acagtcaatt caaagatatg gaaatggtca aaaatatatt agacggtatc
    66901 tcattggata acttgattgg accattcttg tggtattctc ttctattcat gatctttggc
    66961 attttgattt tttctgtttt agcagcttta tgtggctctt tggtcaacaa agcagaagat
    67021 acagcaaaag caattttgcc agtgacttat ctatctctag gcggatatat gcttggattg
    67081 atactaggag cttcagatcc aaacaatatc gttattcgga tcacttcgta tattccattc
    67141 ttatcttcat atattatgcc ggttcgctta gcaaatgaga cagtcggtat gggtggagca
    67201 ttgatttcct tgttggtact tgtagccgta acattcttat tgatgatctt gtctgcaaac
    67261 atgtacaaat cgaatgtact cgtgtatagc gaaggcggat tatggagttc attgaaacaa
    67321 tcgatttcaa ttatgagaaa tgaacgaaaa aaagcataaa atatagaaaa atatagaagg
    67381 aagctggaca tttattgttc ccagcttctt ttttgtatag acctatttct atttttttat
    67441 tatattggat atatatattc attgaaaata gagcataaca actttataat gttgagtata
    67501 ccaatttttg gaggcgtttt aatggaccgc tatttagatt tactttcaga gaagttccct
    67561 acaacggaag atgtcatcac ggaaatcatc aatctcgaag caattttgca gcttccaaaa
    67621 ggaacagagt tatttttgag cgatatacat ggcgaatttc ctgcttttga tcatattctg
    67681 cgtatcggtt caggaaatct taaagaaaag atcaaagacc ttttctctga acaaatgacg
    67741 gaaacggaga tcagccaatt cactattttt acggcttatc cagaatatgt gttgacgact
    67801 gattggtaca aaaaacaaga taaatcccag ctgatccatc gtttgatcga tcttttacgg
    67861 tttacgacag tcaaatacac tcgatcaaaa gttcgaaaag gattgccgaa agaatatagc
    67921 tatatcattg aggaactctt gtatatcgat gatcgtatca acgggaaaaa agactatgta
    67981 aaaaaaatca ttgaacaact tgtcttgatg aaggaagaag aaaaattttt aacaaaactt
    68041 gcgcagacga tccaaaaatc tgtcatcgat catttgcata tcgtgggtga tatttttgat
    68101 cgaggaactc aagcagccaa agtaatggat cgaatcatgg atttttcctc tgtagatatc
    68161 cagtggggga atcatgatat tttgtggatt ggtgcgtatt gcggctctga ggcatgttta
    68221 ttgacgcttt tacggattgc cgcacgctac aactatttat ttgaattaga gaaagaatat
    68281 ggattaaatt tacgtccatt attttctttt gcccatcaaa cctatcagaa gaatccagta
    68341 tttaccccta aaaacaggaa agatctatct gaaagagagc aagaagaatt agaaaagatc
    68401 catcaagcac tttctatcct ccaattcaaa tcagaacatc agttgttgaa tcgacgacct
    68461 gaatttaaaa tggacgatcg taaattgttg gaaaaaatca actatgagga atcaacgatc
    68521 gttttagaag gtcagctcta tgagttgaaa gggacttgtt ttcaaacgat ccagccagat
    68581 gatcctaaaa aattcttgga agaggaacaa aaagtgatcg atagtttgat gtattcattc
    68641 cagcactcgt taagaatgca aaaacacatg acgtttttga tcgaaaaagg gggaatgtat
    68701 ctcacgaaca atcagcatgt actgttccat ggctgtatac ctgtagatga aaaggggcag
    68761 ctcttagcat ttgaattaga ccaatcttat cgcggaaaag aactgctggg attttttgaa
    68821 aaacagataa ctgaaagcgc aaaagatttg actgcaaatg aagatttggc gaccgatttg
    68881 atttggtatg cgtggagcgg gccattttcg cctttattcg gcaagagtaa aatggcaact
    68941 tttgagcgct attttctcac agagcctcat acgcacgtcg aaaaaagcaa tccctattat
    69001 cacttgcgag atgaagagtg gttcagtgaa aaaattttgg cagaattcga tgcaaaagaa
    69061 gaaggttcag caatcatcaa tggacacacg ccagtaaaag taaaaaaagg agagtcaccg
    69121 atcaaagcaa acggaaaagc atttgttatt gacggaggtc tatccaaagc ttatcaaaaa
    69181 acaaccggga ttgctggcta ctcattacta tgcaattctt acggtttcca aatcgtgaca
    69241 cattatccgt tcttgcctat tgaacaacaa tttgcaaaaa aatctgatca aacgaatgta
    69301 aaaaaagtga tcgagcagca gctcccacgc aaacttaatc ggaatacgac aaaaggaatg
    69361 gaattacagc aacagattac tcaactccaa aggctgttgg attatcgaaa ggacgattga
    69421 gaaaaatgct agccaacatt gctcaataga ctaaaaagcg actgcaacaa aaggcgtgtc
    69481 gcagtctcct tcggaattag ctcaaaaaac ggtaaaatat tagaagctat ttttctgttt
    69541 atttctctaa tactcaggtc agggcgctga tttcacgacc tcttccttaa acggtgttct
    69601 cctgccaact cctcggtcac aagtcacaat atccaacaaa catgagaagc tgttttttga
    69661 aattgctcct tgtgactcag agttgaacgg caggctcacg acctaatcag aaaaacgatt
    69721 aaaattcatt cacaaagaaa tcgaataaac gctgttcaga tgaatctaca cgatgagaaa
    69781 gttccggatg ccattggaca ccaaggattt tgtgcatatt atctttactt tggactgctt
    69841 caatgagtcc atcgttggaa aaggcgatgg cttttaacaa aggagaaaga tctttgactg
    69901 cctgatgatg ataggaattc acaaagtatg aatcagggag aaggtcactt aataaactgt
    69961 ccgaaacgat tttgacttcg tgagttgcaa actgcggggc agtgggctgt tgttcgtgtt
    70021 ttacggacca ttttggatat aatgaaagat cttgataaag tgttccctcc aacacgacat
    70081 tcaatagttg aagtcctcgg cagactgcaa aaatcggttt tctttgtttc aatgcttcca
    70141 agatcaatgc ctgttcgaat tggtctcgtt gaatatttgt ttccgtcagt tttggatgag
    70201 ggtcttccat gtaaagttgc ggactaacat cctgaccgcc tgcaagaatg agtttgtcga
    70261 ttttctcaat tagttgaggc gctgaatctg gagacatgac tggcagaata agaggtaatc
    70321 cttgagcatc taaaactgca tccacaaatc cctgcggtgt atagcttact tgatttcctt
    70381 ggaaagtatc tgtcgctcga atcagttgat tcccggcaat gccgatgatt ggtttcattt
    70441 ttattccccc taaagtagac taattgtaac aaggatttaa aaaaatttaa actatgtaag
    70501 acttgcccct atacttatct gttcttgctg tgttactatc tgtaaagaga attttcgtgt
    70561 gaaattcatg acgatagttg aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa
    70621 ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc aaggggtgag aaggacattt
    70681 tattgaaggg gcctgcctat actaagttat ctttagaggt acatactttc aatgaaaaaa
    70741 gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag caagtgttag aagctttgga
    70801 acaaatccga aaagcagaag aagaaaatga gaagcgtgct cgaaccctac ggcagcagtt
    70861 gaagacctat gcagaagaaa aagagcaaga attggagata atccaaacga acagtcggca
    70921 acagctgaaa cagttggtag aagaaaaaga acaattcgaa aagcaagaat tagaaaaaga
    70981 aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca actttgaaag accaatatca
    71041 aaagcatcag agtcaagcaa tcggtgcaat cattgaaagg gtgaaagaaa cttatggccg
    71101 tcactaagat ggaaaaagtg accctcattt cagataaaag aaatcaggag aggattttac
    71161 aagcagtaca aggaatccaa aatgttgaaa tccgggatct gtatcaaaat agaacaaaca
    71221 accagtgggt agaaaaatat tttccagata ccacgattat tgataaagaa gcaaaattaa
    71281 gcgctttagg caatcggctg aaagacatcc aagaagctat ccaattcata caacaccacg
    71341 gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga actttcctta caagacttgg
    71401 aagcaaatta ttccgaagaa aatttcatga ataaattaga agaagtcctt gatttaaaag
    71461 aaaagtggga acagctaact gaaaaacgtg atcgtttaga agaagaggaa gaatggcttc
    71521 ttcattggca acagctggat attgctccaa gtatgtatgc aagtaagacg accatttttg
    71581 agatggggac tgtgagtgca gcaaattttg aaagtttcaa ggaagaacta agtaagcttg
    71641 atgacgttta tatggaagaa atagattata cgccaaaaca agtttatctt tcgtatgttg
    71701 ctttaaaaaa atcagttgct gctatagaag aaattgctgg gaaatacggg ttcaataaag
    71761 aaacgtatcc ttatgaacaa aatcctaaag aaaaattgca agaaaccaaa cagcaattat
    71821 ccattgttca cgagcagcaa aaaaagataa cgacagcgtt aggtcagtgc agcggctata
    71881 tccaagaatt tgaatgggta gaagaagtca cacttgccgt tgcagaacgg gaaaaagtaa
    71941 aagaacggtt tatccatgcc acttatctga tcgttttgca aggttgggta gatgcagaag
    72001 aaaaacagaa tttgatgcat gtgttaaacg aaactgtatc agaagaagat atatacgtta
    72061 gtttcgaaac accaaactca gaagagattc agcaggaagt ccctactaaa ttgaaaaacc
    72121 atccgcttat cgaacctttt gaattgctga ctgaaatgta cagtctgcca aaatatgaag
    72181 aagtagatcc aacgccttgg atgacaccat tttatcttgt cttcttcggg atgatggtcg
    72241 cagatgttgg ttacggattg ctgatgctca ttgggacaat actagctcaa aaactatttg
    72301 ttttaccccg cgggatgaaa cgtttcgtaa aattctttga gattctctcg gtaccatcga
    72361 tcatctgggg attgatctac agttcatttt tcggacaggc attaccaaaa gaactatttg
    72421 gtatccgctt gcctttccca atcctatcta cgacggatga cgtcaatacg attttgattt
    72481 tatccgtgat tttcggtctg atacagatct tggttggatt attcgtggca gcaaaggaaa
    72541 atatcaggag gaaagattac ttgagtgctg tcagtgatgg ttttgcctgg caagggatcc
    72601 tgattgggat cgttattgca ttgattggtg tcatgttgat aaaaaataca gcatttgttt
    72661 atcttggcgc ggggctatcg atcattgctg ctttatgtat cttaatcgtg ccgattatcc
    72721 aatcgccgtc taaagtcaag ggtgctgcca aaggagcgta taatctttac ggattgactg
    72781 gttatatcgg tgatttagtc agttatacac gtttgatggc actaggtatt tcaggcggaa
    72841 gtattggcgc ggcattcaat atgctagttg ctttcatgcc gccagccgct cgattcagtg
    72901 tagggatcct tttgatcatc gctttacacg cactgaatat gtttttgaca ctgttaagtg
    72961 cctatgtcca tggtgcacga ttgcagtatg tcgaattttt tggaaaattt tatacaggag
    73021 gcggacgtgc ctttgacccg ctgaaaacgg cggaaaaata cgtcaacatc aatcataaga
    73081 aaaaaaacaa taaataattg gaggattttt atacaatgat ggattactta attagtcaaa
    73141 atggtggaat ggtatttgca gtattagcga tggcaacagc gacgattttt tcagggattg
    73201 gttcagctaa aggtgttgga atgacaggtg aggcagcagc agcgttaaca acaagtcaac
    73261 ccgaaaaatt cggacaggct ttgattctgc aattgcttcc aggtacgcaa gggttgtatg
    73321 gattcgttat cgcctttttg atttttatca acttaggaaa tgatatgtca gttgtacaag
    73381 gattgaactt tctaggcgct tctctgccga ttgcttttac aggtttgttt tcaggtatcg
    73441 cccaagggaa agttgcagca gcaggtatcc aaattttagc gaaaaaacct gaacatgcta
    73501 caaaaggaat catttttgct gcgatggttg aaacatatgc gattttaggt ttcgttattt
    73561 ccttcctatt agtattgaat gcctaagcat aaaggaggaa aagcagatga acgccattga
    73621 aaaaatcatc tctcagatga atgaagcagc agagcaagaa agagcggcgt tagagcaaga
    73681 agagcgaatg aagatcgatc aaaactttga acaaaaacgt acgcaaatcg aaacagaaca
    73741 tcaaaaacaa aaagaaaaac aaatcgaatt acttgaaaaa aaataccgtc agcttaggaa
    73801 tcgccaacaa gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat ttttacgacg
    73861 attgtttgca gatgcaatag ctgaaatgga gaattgggat gaatctgaac aaatccagtt
    73921 tatcaaaaat tcattgtatt cattaccttt gacaggcaaa gtaacattca ttgctggaga
    73981 aaaatcagca gcttatcttt ctcagacatt gctggatgaa tggaacaatg aacttccctt
    74041 tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg ggttttctga taaacgatca
    74101 aggagttcag tataattttc ttttcagcag ccttgtacaa gatatccaag gaacgatgag
    74161 ttttgagatt gccaatcaat tattcgaata aggagggatc agtgtatgga ataccacgaa
    74221 ctaaatccct tgattcgcgg aagagagctt gaacttttgt ccaatgagac atttgaacgg
    74281 atgattcaaa caaattctgt cgaagcactt ggtgaaatat tgaaatccac gatttacagt
    74341 ccatatattt atgacggata tgaatatgac ttcgaacaga atcttttaaa ggagcagagc
    74401 cgtctatttc gctggttaaa ggaatcagca ccggagcctg aaatcgtttg gatctatacg
    74461 atgcgctata ctttccataa tctgaaggtt ttgacaaaag ccgagatgac aaatcaaaat
    74521 ctggaccatt tgtatatcga cgatggattt tattcattag aaaccttgaa ggatgccatt
    74581 catacgcaag tttcaaatga actccctgaa agcattatgg acagtatacg agaagtacgt
    74641 gattattttg aagagtctac cattcttcag ggaattgacg tgatttatga tcgacagttt
    74701 ttgacagagc agcgacggct tgccgataag ctaggatatc aagaattgct gacagaagtc
    74761 attgctttta ttgacttgac caatatcaca acgatggctc gaggaatatt gcagaaaaga
    74821 acatatgggt tcatgacgac ggtactttcc agctcgggaa gtatcgaaaa agaagtattt
    74881 ttgaactttg tagataaaga tatggaagat tacacgaatt ttctattaga gacagattat
    74941 gctgattttc tgcgccctgt catccgtgag ggaaatcttg atctcgtaaa gcttgaacag
    75001 ttgaaagatg attatctaag ttcattgtat caagaagcac aaacccaggc tttcggacca
    75061 ctgccactac tagccttttt gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg
    75121 gttatcggca aaagaagcca tttcgaacca gaaacaatca gaaagcgggt gagacaagtc
    75181 tatgacgcat aagatcggtg tagttggcga caaagattct gttttgccat tcaaattgtt
    75241 tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat
    75301 ggcaaagaat gagtatggcg tgatttatgt cacagagcaa tgtgcagcat tgagcccaga
    75361 aacgatcgaa cgttacaagg aacaaatgat acctgctatc gtattgattc ccaatcatca
    75421 aggatcatta gggatagggc tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca
    75481 aaatatctta tagtatgaaa aggagataga aattttgcaa attggaaaaa tcataaaagt
    75541 ctccggacct cttgtaatgg ctgagaacat gtctgatgcc agtatccaag acatgtgtct
    75601 agtgggagat ctaggagtta tcggagaaat tatcgagatg cgtggcgatg tggcgtctat
    75661 tcaagtatat gaagagactt ctggaattgg tccaggcgaa ccggttcgct ctactggtga
    75721 agctttatct gttgaattag gaccagggat catttcacaa atgtttgacg gaatccaacg
    75781 gccattagat acctttatgg aaatcacaca aagtaatttc ttagggcgcg gagttcaatt
    75841 gcccgcattg gaccatgaga aaaaatggtg gttcgaacca acagtagaag ccggagaaac
    75901 ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca aaagttataa aacataaaat
    75961 catggtgcca aatgggataa aaggaacgat caaaacaatc gaaaaaggtt cattcactat
    76021 tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac aaaaatttga cgatgcttca
    76081 aaaatggcca gtaagacgta gtcggccaat caaagaaaaa ttaaacccgg atgcacccat
    76141 gatcactgga cagcgagtga ttgatacatt cttcccagtg acaaaaggag gagcagcagc
    76201 tgtacctggt ccttttggtg cagggaaaac agtcgttcag catcaaattg caaaatgggc
    76261 cgatgtggac atggttgtct atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt
    76321 tttaaatgaa ttccctgaat tgattgaccc taatacagga gaatcgttga tggaacgtac
    76381 ggtattgatt gctaatacat caaacatgcc agtagcagct cgagaagctt ctatctatac
    76441 gggaatcaca attgcggaat atttccgtga catgggttat gacgttgcta tcatggcaga
    76501 ctccacttca cgatgggcag aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc
    76561 tggtgatgaa ggttatccag cttatcttgg ttcacggctt gcggaatact atgaacgatc
    76621 tggaagagtc attgcattag gatcagaagg tcgcgaagga agtataaccg caatcagtgc
    76681 tgtatccccg tctggagggg atatttcgga accagtcaca caaaatacat tgcgggtcgt
    76741 aaaagtattc tggggtcttg attccagttt agcccaaaaa agacattttc cttcgatcga
    76801 ttggatacaa agctattctc tttattcaac agaagtaggg aagtatatgg atcatatcct
    76861 tcaagctgac tgggcatcca tggtcaccga gggcatgcgt attttgcagg aagaacagca
    76921 gctgaatgaa atcgtgcgtt tggtagggat tgattccctt tcagataatg accgtctgac
    76981 attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt
    77041 cgatacattc acttcaagag agaaacaatt caaaatgttg aagatgatct tgacctttgg
    77101 tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta agtgagatca tggacggtac
    77161 cgtatcgatt cgtgaaagga tcggacgaag caaatatatc ccggaaacag aattagaaaa
    77221 actagatcaa attattggtg aaatcaagca aacgatgcaa acgatcgttt cagaaggagg
    77281 catgagcaat gattaaagaa tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg
    77341 ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga agtccgtatg caaaatggcg
    77401 agattcgtcg tggacaagta ttggaagtcc aagaagataa agcaatggtc caaatattcg
    77461 aaggaacaag tggtatcaat ttacgtgact cttctgtacg atttttaggc catcctttag
    77521 aactaggtgt atcggaagac atgatcggtc gagtattcga tggtttggga cgaccgaaag
    77581 ataacggacc agagatcctg cctgaaaagc atttggatat taacggagaa gtaatcaatc
    77641 cagtagctcg ggattatcca gatgagttta tccaaacagg tatctctgca atcgatcatt
    77701 tgaatacatt agttcgtggg caaaaattac cagtattttc tggctctggt cttcctcata
    77761 aagaattagc tgctcagatc gcgcgacaag caacggtact cgattcatca gatgatttcg
    77821 ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc tgaatttttc atggaagatt
    77881 ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat gaatttagca aacgaccctg
    77941 caatcgaacg tatcgctact cctcgtatgg ctttgactgc tgcagaatat cttgcttatg
    78001 aaaagggaat gcatgttttg gttatcatga cagacatgac caactatgca gaggcattgc
    78061 gagagatttc tgcagcacgg cgagaagttc caggaagaag aggatatcct gggtatcttt
    78121 acacgaactt agctacttta ttcgaacgag caggaagaat ccgcggacta aaaggatcgg
    78181 ttactcaaat tccgattcta acaatgccag aagacgataa aactcatcca atccctgact
    78241 tgactggtta tattacagaa ggacagatca tcttgactag agagctttac aagagtggga
    78301 ttcagccacc aattgatgta ctcccgtctc tttcgcggtt aaaagataag ggaactggac
    78361 ccggaaagac aagagaagac catgcagcta cgatgaatca gctttttgca gcctatgcac
    78421 aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga atctgcttta tctgatgtag
    78481 acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga atatgtcaat caaggattct
    78541 acacgaaccg ttcgattact gaaacacttg atctagggtg ggaactgcta tcgatgttgc
    78601 caagaaccga attaaaacga atcaaagacg acatgctgga caaatatctg ccagaaggga
    78661 agtgagttct gatggcacga ttgaatgtga atccgacccg catggaattg actcgattga
    78721 aaaaacagtt gacaactgcc acaagagggc ataaattatt aaaagataag caagatgaac
    78781 tgatgcgtca attcatcctg ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag
    78841 agtctatgac agaagcaatg tctgattttg tattggcaaa tgcttcgatc acggaacctt
    78901 tcatcgaaga attatttgtt ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa
    78961 atatcatgag tgtaaaagta cctgtgatga attttgaata tgatgaacag ctgacagaca
    79021 cacctttgga atacgggtat ttgaattcca gtgccgaact ggatcggtca atcgatcggt
    79081 ttactaccct gcttccaaaa cttttggaac tgacagaaat cgaaaaaact tgtcaactga
    79141 tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc attagaatat atgacgatcc
    79201 ctcaattaga agaaacgatc tattatattc gtatgaagct ggaagaaaac gagcgcgcag
    79261 aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac tgactaagtg gtatcttttt
    79321 ttaaaagagg aaaacgatga ttaaacaaag caaacaaata aagaaacgtt tgccggctgt
    79381 tcagcttctc gcattaggat tttttctcct gattttagtt ggcggcggcc tacttaccct
    79441 accatttttt agtaatagtg ggcatgggac aaattttatc gatgcgcttt tcactgcaac
    79501 ttctgctgtc tgtgtaacgg gattgacaac attgaataca gcagagcatt ggaacagtgc
    79561 tggtcaattt ttgatcatgc ttttgattga gatcggcggt cttggtttta tgatggttcc
    79621 gattctatgc tttgcattga taaagaagaa agtcagtctc agcacacgta ttatattaaa
    79681 agaagcgctg aatctagaag aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa
    79741 attggccttt gttatccaat tgattggtac ggttgcttta tcttttgtat ttgtgcctga
    79801 atttggctgg ttgaaaggta tctggtacag tttgttccat gcaatctcta gtttttgtaa
    79861 tgcagggttt gatttgttag gagacagttt agccaatcac cagcaaaatg tttatttgat
    79921 catggtggta tctgctttga tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt
    79981 actggaatat catcagaaaa agaagatgac gattcattca aaaatcgcat taaccgttac
    80041 aggtaccctt ttattaggcg gattcgttgt gttctttttc acagagcgca atggggcaac
    80101 gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc
    80161 tagaacggct ggatattatt cagttgatta cctccatatg agccatgcag gtctgatttt
    80221 aacgatgttt ttgatgtaca tcggcggaac atctgggtcg acggccggcg gattgaaaac
    80281 aactacgtta ggtgtgttgc tgatccagat gcgttcgatg ttgaaaggac gtacgagagc
    80341 ggaagcattt ggccgtacca tccgacaagg cgcagtgttg cgggcactta ctttattttt
    80401 tgttacgttg acgttgtgtg tagtcgccat catgatttta tcggtgactg aaacgatacc
    80461 taaaacttct ggcatcgaat atattgcttt tgaggtcttc tctgcgtttg gtacagtagg
    80521 tcttacgatg ggattgacgc cagacttgac tgcaataggg aaattgatca ttatagcgct
    80581 tatgtacatt ggacgagttg gtgtattgac tgtggtcttc tctctgatga caaaagcaca
    80641 aagaacacaa tcaaaataca aatatccaga agaaagtgtc atgattggat aatttcatct
    80701 tctgacagaa acctagtgat ttagtaaaat aagaaaataa aataattttt cttgcttctt
    80761 ggatacttta agtgcaaaat gttcgtttat tttttgaggc actacccgtt gttgaacctc
    80821 gggtagtgcc tcttttttat tgaaaggaat caataagatt tgattccttt atttatcaat
    80881 aggttataat tcatattttt ttattttttc ttttacgata cttagtccag aagctaaaat
    80941 ttcaaggtta ttagaggata gggaaattcg aacgaatgca tctggttgcg cacgtgtagt
    81001 aaagcgtctg gaatgataaa gtcggatacc gttagcaaga aagtcattct caattatctt
    81061 agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa gggacacgat tcgttggttt
    81121 ttctactttg aaaagagaat caaaaagctg attggctctt tttgtaagac ttaatttagt
    81181 ttctaagatc ctatttgctt catttgaatg gaggatttga gtgatgactt ccacatctaa
    81241 tccagaagtt ttcacattta tattaaaaat tgcttgttct atcttgcttt ggacatgtac
    81301 tggaactaca agataagcaa ctcgcaatcc agaacaaata aattttgtca ttccagctaa
    81361 gtagattgtt tggttaggta ataaattttg gaaaggagga atgatcgtat gttgatagta
    81421 agttgttaaa aatgaatgga tatcatcttc aataacccat agaccttctt tttcaatcac
    81481 ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac cctataggat tattacaaga
    81541 aggcataaga aagacaccat gaatcttctt ttttctacat tcctgaatca atatatcagg
    81601 tcgcattcct tgctcgtcaa aatcaattgg aacgatctct aaatgatata actgagccaa
    81661 ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt ctatcacctg gagagaaaag
    81721 tgcagaaagt gtgatagcca atccattttg aacaccggaa acaatagata gagattctaa
    81781 ggacggaatg actccttgag tttttaacca atttgctgca gtagtcaatt gaaaatttgt
    81841 gcctttggga tcttggtaat taagcaaacc aacaagattt gggttattac tagttgattg
    81901 aataaacggg acaacgaatt tattacattc ttcaaaagaa ctaaccagac caaaatcaat
    81961 gatatgttcg gttacttcat tggtagagat ggtgatggac tcaaagacat tgggagaaat
    82021 aaatgtgcca cttccaatat tcgtatacag caaacctttc tcaattccat atttataagc
    82081 cttgccaacg gtagtaaaat ttaaatctaa ataatcagct agctctcttt gggagggtaa
    82141 ctgagtgttt ttctgtaatt tattctttaa aatatcttgt tctaattgtt gaatcaacga
    82201 tcgataataa ggacgcttca atttttgttt gtctggtcgc caagttaacg ggtaattgtc
    82261 ataagaatta ataggcattt tctccactcc ttaaaat
//
LOCUS       DARBER010000004        76440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARBER010000004 DARBER010000000
VERSION     DARBER010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.004"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            71..1129
                     /locus_tag="SCK06_000271"
     CDS             71..1129
                     /locus_tag="SCK06_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG1150303.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1133..2167
                     /locus_tag="SCK06_000272"
     CDS             1133..2167
                     /locus_tag="SCK06_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG1150304.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2269..2985)
                     /locus_tag="SCK06_000273"
     CDS             complement(2269..2985)
                     /locus_tag="SCK06_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1150305.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(2954..3739)
                     /locus_tag="SCK06_000274"
     CDS             complement(2954..3739)
                     /locus_tag="SCK06_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG1150306.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3730..4479)
                     /locus_tag="SCK06_000275"
     CDS             complement(3730..4479)
                     /locus_tag="SCK06_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1150307.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4777..5301
                     /locus_tag="SCK06_000276"
     CDS             4777..5301
                     /locus_tag="SCK06_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG1150308.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5317..6816
                     /locus_tag="SCK06_000277"
     CDS             5317..6816
                     /locus_tag="SCK06_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG1150309.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6904..7506)
                     /locus_tag="SCK06_000278"
     CDS             complement(6904..7506)
                     /locus_tag="SCK06_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1150310.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7610..8566
                     /locus_tag="SCK06_000279"
     CDS             7610..8566
                     /locus_tag="SCK06_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG1150311.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8871..9827)
                     /locus_tag="SCK06_000280"
     CDS             complement(8871..9827)
                     /locus_tag="SCK06_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1150312.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10001..10561
                     /locus_tag="SCK06_000281"
     CDS             10001..10561
                     /locus_tag="SCK06_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150313.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10554..11378
                     /locus_tag="SCK06_000282"
     CDS             10554..11378
                     /locus_tag="SCK06_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG1150314.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11388..12005
                     /locus_tag="SCK06_000283"
     CDS             11388..12005
                     /locus_tag="SCK06_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1150315.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12023..12706
                     /locus_tag="SCK06_000284"
     CDS             12023..12706
                     /locus_tag="SCK06_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG1150316.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13389..14624
                     /locus_tag="SCK06_000285"
                     /pseudo
     CDS             13389..14624
                     /locus_tag="SCK06_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            14632..15519
                     /locus_tag="SCK06_000286"
     CDS             14632..15519
                     /locus_tag="SCK06_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1150317.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15536..16372
                     /locus_tag="SCK06_000287"
     CDS             15536..16372
                     /locus_tag="SCK06_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1150318.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16375..16998
                     /locus_tag="SCK06_000288"
     CDS             16375..16998
                     /locus_tag="SCK06_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150319.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16995..18287
                     /locus_tag="SCK06_000289"
     CDS             16995..18287
                     /locus_tag="SCK06_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1150320.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18646..18743
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18859..19440
                     /locus_tag="SCK06_000290"
     CDS             18859..19440
                     /locus_tag="SCK06_000290"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG1150321.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19443..20750
                     /locus_tag="SCK06_000291"
     CDS             19443..20750
                     /locus_tag="SCK06_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG1150322.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21043..21531
                     /gene="purE"
                     /locus_tag="SCK06_000292"
     CDS             21043..21531
                     /gene="purE"
                     /locus_tag="SCK06_000292"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG1150323.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21524..22648
                     /gene="purK"
                     /locus_tag="SCK06_000293"
     CDS             21524..22648
                     /gene="purK"
                     /locus_tag="SCK06_000293"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG1150324.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22661..23956
                     /gene="purB"
                     /locus_tag="SCK06_000294"
     CDS             22661..23956
                     /gene="purB"
                     /locus_tag="SCK06_000294"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG1150325.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24136..24513)
                     /locus_tag="SCK06_000295"
     CDS             complement(24136..24513)
                     /locus_tag="SCK06_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150326.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24769..25497
                     /locus_tag="SCK06_000296"
     CDS             24769..25497
                     /locus_tag="SCK06_000296"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG1150327.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25497..25751
                     /gene="purS"
                     /locus_tag="SCK06_000297"
     CDS             25497..25751
                     /gene="purS"
                     /locus_tag="SCK06_000297"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG1150328.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25753..26424
                     /gene="purQ"
                     /locus_tag="SCK06_000298"
     CDS             25753..26424
                     /gene="purQ"
                     /locus_tag="SCK06_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG1150329.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26425..28647
                     /gene="purL"
                     /locus_tag="SCK06_000299"
     CDS             26425..28647
                     /gene="purL"
                     /locus_tag="SCK06_000299"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG1150330.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28632..30071
                     /locus_tag="SCK06_000300"
     CDS             28632..30071
                     /locus_tag="SCK06_000300"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG1150331.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30094..31146
                     /locus_tag="SCK06_000301"
     CDS             30094..31146
                     /locus_tag="SCK06_000301"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG1150332.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31143..31721
                     /gene="purN"
                     /locus_tag="SCK06_000302"
     CDS             31143..31721
                     /gene="purN"
                     /locus_tag="SCK06_000302"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG1150333.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31718..33253
                     /gene="purH"
                     /locus_tag="SCK06_000303"
     CDS             31718..33253
                     /gene="purH"
                     /locus_tag="SCK06_000303"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG1150334.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33269..34543
                     /gene="purD"
                     /locus_tag="SCK06_000304"
     CDS             33269..34543
                     /gene="purD"
                     /locus_tag="SCK06_000304"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG1150335.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34665..35579
                     /locus_tag="SCK06_000305"
     CDS             34665..35579
                     /locus_tag="SCK06_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1150336.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35712..37277)
                     /locus_tag="SCK06_000306"
     CDS             complement(35712..37277)
                     /locus_tag="SCK06_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1150337.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37270..38721)
                     /locus_tag="SCK06_000307"
     CDS             complement(37270..38721)
                     /locus_tag="SCK06_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1150338.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38735..39751)
                     /locus_tag="SCK06_000308"
     CDS             complement(38735..39751)
                     /locus_tag="SCK06_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1150339.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            39971..41059
                     /locus_tag="SCK06_000309"
     CDS             39971..41059
                     /locus_tag="SCK06_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG1150340.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41113..42639
                     /locus_tag="SCK06_000310"
     CDS             41113..42639
                     /locus_tag="SCK06_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG1150341.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42641..43846
                     /locus_tag="SCK06_000311"
     CDS             42641..43846
                     /locus_tag="SCK06_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1150342.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44103..45080
                     /gene="guaC"
                     /locus_tag="SCK06_000312"
     CDS             44103..45080
                     /gene="guaC"
                     /locus_tag="SCK06_000312"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG1150343.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45344..46768
                     /locus_tag="SCK06_000313"
     CDS             45344..46768
                     /locus_tag="SCK06_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1150344.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47229..48338
                     /gene="pdhA"
                     /locus_tag="SCK06_000314"
     CDS             47229..48338
                     /gene="pdhA"
                     /locus_tag="SCK06_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG1150345.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48341..49318
                     /locus_tag="SCK06_000315"
     CDS             48341..49318
                     /locus_tag="SCK06_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG1150346.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49343..50986
                     /locus_tag="SCK06_000316"
     CDS             49343..50986
                     /locus_tag="SCK06_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEG1150347.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            50994..52400
                     /gene="lpdA"
                     /locus_tag="SCK06_000317"
     CDS             50994..52400
                     /gene="lpdA"
                     /locus_tag="SCK06_000317"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG1150348.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52562..52837
                     /locus_tag="SCK06_000318"
     CDS             52562..52837
                     /locus_tag="SCK06_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG1150349.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            52843..53610
                     /locus_tag="SCK06_000319"
     CDS             52843..53610
                     /locus_tag="SCK06_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG1150350.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            53778..55607
                     /gene="typA"
                     /locus_tag="SCK06_000320"
     CDS             53778..55607
                     /gene="typA"
                     /locus_tag="SCK06_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG1150351.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            55868..57640
                     /gene="recQ"
                     /locus_tag="SCK06_000321"
     CDS             55868..57640
                     /gene="recQ"
                     /locus_tag="SCK06_000321"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG1150352.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            57754..58056
                     /locus_tag="SCK06_000322"
     CDS             57754..58056
                     /locus_tag="SCK06_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG1150353.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58059..59222
                     /locus_tag="SCK06_000323"
     CDS             58059..59222
                     /locus_tag="SCK06_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1150354.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59248..62676
                     /locus_tag="SCK06_000324"
     CDS             59248..62676
                     /locus_tag="SCK06_000324"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG1150355.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            62697..63821
                     /locus_tag="SCK06_000325"
     CDS             62697..63821
                     /locus_tag="SCK06_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150356.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            63811..64248
                     /locus_tag="SCK06_000326"
     CDS             63811..64248
                     /locus_tag="SCK06_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1150357.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64232..64570
                     /locus_tag="SCK06_000327"
     CDS             64232..64570
                     /locus_tag="SCK06_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG1150358.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            64689..65249
                     /gene="rsmD"
                     /locus_tag="SCK06_000328"
     CDS             64689..65249
                     /gene="rsmD"
                     /locus_tag="SCK06_000328"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG1150359.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65246..65737
                     /gene="coaD"
                     /locus_tag="SCK06_000329"
     CDS             65246..65737
                     /gene="coaD"
                     /locus_tag="SCK06_000329"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG1150360.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            65727..66770
                     /locus_tag="SCK06_000330"
     CDS             65727..66770
                     /locus_tag="SCK06_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG1150361.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            66929..67657
                     /locus_tag="SCK06_000331"
     CDS             66929..67657
                     /locus_tag="SCK06_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG1150362.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            67773..68018
                     /locus_tag="SCK06_000332"
     CDS             67773..68018
                     /locus_tag="SCK06_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1150363.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            68015..68233
                     /locus_tag="SCK06_000333"
     CDS             68015..68233
                     /locus_tag="SCK06_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG1150364.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68369..68908
                     /locus_tag="SCK06_000334"
     CDS             68369..68908
                     /locus_tag="SCK06_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG1150365.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            68901..71174
                     /locus_tag="SCK06_000335"
     CDS             68901..71174
                     /locus_tag="SCK06_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG1150366.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71212..72258
                     /gene="holA"
                     /locus_tag="SCK06_000336"
     CDS             71212..72258
                     /gene="holA"
                     /locus_tag="SCK06_000336"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG1150367.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72347..72601)
                     /locus_tag="SCK06_000337"
     CDS             complement(72347..72601)
                     /locus_tag="SCK06_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG1150368.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(73001..73270)
                     /locus_tag="SCK06_000338"
     CDS             complement(73001..73270)
                     /locus_tag="SCK06_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150369.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(73740..74195)
                     /locus_tag="SCK06_000339"
     CDS             complement(73740..74195)
                     /locus_tag="SCK06_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG1150370.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74333..76261)
                     /locus_tag="SCK06_000340"
     CDS             complement(74333..76261)
                     /locus_tag="SCK06_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1150371.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        24770 a        13199 c        16596 g        21875 t
ORIGIN      
        1 tgttaatacg tattagcaaa ataaaagctt tttttattgt gaataaatga acataattag
       61 gaggagaaag atgaacttat ttgacatttt tcgatcgact ttgacagata tgtcgattgt
      121 cagtgcaatc acttcaacag tttttattat attattaggg tttgtttgta gaaaaaaggg
      181 gattttttct gaatctgttg gaaaaattct atcgaaagtc gtcttaacag tggctttgcc
      241 agcactggca tttcgagctt tcatgcaaga tatcaataca gaattactga tgcaaggaat
      301 caatgtcttg atttggggat tagttattta tattattctt attctagttt caaaaccttt
      361 tttcttaagc tataaagggg ataagcaaac aacgttgcgt gtcctgacga tctttggttc
      421 tacgaccttt ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta
      481 ttcctcaatt ttcaatattg gctaccgaat ctttttgtat tcttatggat acataaaaat
      541 gagtggcttg aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc caatcattat
      601 cgctactttt gccggccttt tcatttggct gttccaaggg tatctgccac aagtgtcagt
      661 tacaggtgca gaaggagaga taagccaagt agcgtttttg cgaattgacc agactgcacc
      721 gtggctattt cagccgatgg attatttagc tggtttaagt tcaccattgg cgtggctagc
      781 aatcggcgca acattaggtg aagtaagctt caaaaaagct gcatctgaaa aaacttcatg
      841 gtattacagt ctagtcaaag tggtatttgt acctgcattg aatatcgtga ttctagcagt
      901 tttgacggca acgaatattc taccagtcaa ttttgaagca ctggcaacaa tcgttatcat
      961 gatggctaca ccagctgcaa cagtggcagc agcctatgct atcagttttg atagggaggc
     1021 acttttagcc tccaatgcat ccttactgtc aactattttt gctgtggtac tgacacctgt
     1081 atggatcgtt gtattacaaa tgattcatgg cataggttta ttttcataag aaatggagga
     1141 agaaagtatg agaatcgcat gttacggtgt tcgtccgaac gagatagatt tttttgaaaa
     1201 attgaatatt taccattatg atctcagtct ttatgaagaa ctgttgacac atgacaatat
     1261 cgtgtcagca aaggaccatg atgctgtatt gttgagggga aattgtatag cagatgagac
     1321 caatcttgca aaaatgcagg aatatgggat tcgttatgtt tttacacgga ctgtgggagt
     1381 caatcatatc gatctacaag cggcagctga ttttggtatg acagtagccc gtgtaccatc
     1441 gtattcgcca aatgcgatag cagagttatc tttgactttt gccatgatgc ttttgagaaa
     1501 tactgcgtat acgacgatcc gaacatcttt caaagatttt cgagtggatg agcaaatgtt
     1561 tagtcgtgaa atcagaaatt gcacagtagg gatcatagga acaggcagga tagggttgac
     1621 ggaagctgtt ttgtttaagg gactaggagc caaagtgatc ggctacgata ttcatcagac
     1681 agaagctgct aaaaaagtat tgacgttcca ctcgcttgaa gagttattag ccaaaagcga
     1741 tattgtcagt attcatgtac ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg
     1801 atttttagct caaatgaaaa aaggtagcat cttgattaat acagcccgtg gagagcttca
     1861 agataatgaa gctattttaa gagctttgaa aagcaatcat ttagctggtt ttgcaaccga
     1921 tgtttttgca aatgaaacag aggttttttt ccgatccttc aagccatggg agacgattcc
     1981 agaccctgct atccagcaat tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt
     2041 aggttcaaat acagatgagg cattatcaaa tatgatttcg acaagtttcg agaactttcg
     2101 tgaaataata gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt
     2161 aagataagaa aacatggata ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt
     2221 tgttacttct gtatacttgg actattttgt atgggataat tcgatgactc aacagtaatc
     2281 tgtgagcttt ttccgcccat tctttgtcaa ttcataacag gttagcggct ggccgatttc
     2341 caaatatttg attctttcgt tcaatatatc ttgttctact aaaaaattca aatatttctt
     2401 gacggatata cgtgacagtg agcttttttg agccagttct tttgttgaaa aaatagtatc
     2461 ttcttttaga ataaatgcac ttattttttt taaagtcagt tttgacagac ctttaggcaa
     2521 atcgatgaat gatggtattt cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa
     2581 tttctgatcg gctttttcag tcgtttggat tgtctgagag aaagcaagat acttttcgat
     2641 cgccagctga aaacgttgca aagtgaacgg tttgatcaaa taatctaagg caccgtagcg
     2701 gatcacattc tttatggttt cgatatcatc tgcagcagtg atcaaaatca ctgggatatc
     2761 atttccttgc tgtctaagcc aagataaaaa atggatacca ctttcgccag gcatgtatac
     2821 atcaagcagc agcagatctg cgtttttttg tctgaaatat ttttttcctt cttctacatt
     2881 tctgaaactt tcgattacgt tcatattagg gatgctttcc acaaaagtac gattcaattc
     2941 tgcaaccatt ggatcatctt cgattatcag gacatttgtc atcattttga tcttcctttt
     3001 ttggtaattc tagatagacg attgtaccgc catttgtttg tttggaaaat tctaaaaatc
     3061 caccgtggcg tttgacgatt gaatggatga gattcaatcc aaacccacga ttgattccct
     3121 tcgtcgaaaa acctagcgta aatatttttt ctgagatttc ttctgatatc ccgacaccgc
     3181 catcgcagac ggtcacgatg aatacttcag cttctgtgtc atagtttaaa tgaatccgga
     3241 cctgtccgtt tctttcatgt ttttgtagca cggcttcttt tgcattatca agtaagttcc
     3301 ctaacacttg caaaagtgaa tggatggagt catccatctc cattttaggg ataaaagaat
     3361 cgctttctaa cagcatttca acagattgtt ctttggcttc gtttattttg cctaataaaa
     3421 aaccggcaat tgcaggtact ttgatcagac ttgagacttg gccaatctct tttttataat
     3481 tttgatttag ctgctggata aaatgggtga cttcttcata cttcttcaaa tcaatcaaac
     3541 cagaaatgac atgaagttta ttcatgaatt catgggtttg agctctcatg gagtttatat
     3601 attgtttcgt ccccccaagc tcctccatta atttcattac ttctgatcga tttcgtaaag
     3661 ttgcgactgc tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta gaaatagttt
     3721 ctatcccatt taaatacatg gattgagtcc gaacggtcgt ttgatgctca atcgtctctt
     3781 taaacaaaac gtcatataga ttatcactga tttttttcct acttcgaaat ccaatagctg
     3841 ctctgtttgg tagaaaatgt tttttgcagg tgtattgatc aagaggatag ttttatcaga
     3901 agagatggct aagattcctt catctacttc attttcgata atcgtttttt cacttaactg
     3961 ttctgctatt tcttgtggtt caagtccaag cagtactttt ttcatttttt gggcaagaaa
     4021 aacggcgcct atgatgccta cgctaagtcc aataatcaat gaaaaaacaa tcattttttg
     4081 tccatctttg acttgattgt caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat
     4141 aatctcttga tcataataaa tagggacgaa atagcggaag ccgaacccta acattccttc
     4201 tttttcagag aaaaattctt gtcctccaat cacttttttc acatcttcat gacttgagaa
     4261 aggaagaccg atgtaacttg ggtctgcgtg agaatatcga atgccttcag ggtccatgac
     4321 aacgataaaa tctaaattcg atgcaatcat gatttcattt gttattcctt gaatcgcatg
     4381 tgtcgggtca ccagtctgca gctttttttg gatgagttta tcattggcaa cgattttagc
     4441 aatgctagag attttatctg acacactaga tcggttcatc gaaagaataa aattcctgat
     4501 caatatggca gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt
     4561 gttctggagc tttaatggtt ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat
     4621 ttattgtgaa aattttaaca tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa
     4681 ccttcctata aaaaacacca ctgagtcggt aaaagaagct atacagaatt ttcaaacatt
     4741 ggtatgatag acgtagaaat ggaaggaggc gtgtatatga tagggggaat cgcacttgag
     4801 acgatttatt ggtatacatt gctgatttgt gcaggattgg cagtcctatt gattattttt
     4861 ggagatgtat tcgattttga tggcccgctt gatccgatgc ttattatccc ttggttaact
     4921 ttcacaagct tatttggttt tctaggtgag cgtttaacag atcaatcatc tttattttta
     4981 tttattgcta gtggaggcat atcgatagtg ttggtatttt tgcttaattt ctatatattg
     5041 atgccgatga aacatgccga atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa
     5101 gtagctgttg tgatcacacc gatccctgta cgaggtatgg gagaaattca gttgaaaagt
     5161 gttaccggtt ccattagtcg tccagcttgt ttttacagcc cccaagaaca gacagctgag
     5221 cggggaacga aagtattgat cattgagatc aaggaaagaa tctgttatgt catgccttat
     5281 gaaagcatga taaaagctta aaggagcgtg tgttaaatgg aaataccagt tgtttttatc
     5341 gcgatcgcaa tcgcagcttt gattttactg atgttgctga tcgtctttgt agcgaagtac
     5401 cagacagcaa aaccggatga agcgttgatc atcagtggaa gttatttagg aagcaaaaat
     5461 gtgcacattg atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc
     5521 ttgccagttt ttcaacgttc gaatcggatc agtctattat cgagtaaatt ggacgtctcc
     5581 acccctgaag tgtataccga acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc
     5641 aaaatcggtt catctgtaga agagattgct acagcagcag aacagttttt aggcaagaca
     5701 agagaagaac tggaaaatga agcaagagaa gtattagaag gacacttgcg ctcgattctt
     5761 ggttctatga ctgtagaaga aatctatcaa aatcgcgata agttcagtca aagcgtacaa
     5821 gaagtcgctt ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa
     5881 gaggtcagag acaaaaacgg ttatcttgac tctttaggga aaccaagaat cgcccaagtc
     5941 aaaagagatg cagatatagc agaagcggaa gctctaaaag aaacgcgtat caaaaaagct
     6001 caagcagaaa aagaatcaca agcagctgaa ttacagcgac aaacagagat cgcagaatca
     6061 ttaaaagaaa aagaactgaa attagcaacc tacaaacaag aacaagatgt tgctaaagca
     6121 aaagcagacc aagcctacaa tttagaaagt gcccgagcac aacaacaagt aatcgaacag
     6181 gaaatgcaga ttaaaatcgt agaacgccaa aaacaaatcg aactggaaga aaaagaaatc
     6241 acacgtcgtg aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac
     6301 gctaaagaac aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa
     6361 cgattccgtg tggaagcact ggcagaagcg gaagccaata agacacgttt agctggacaa
     6421 gcagaagccg aagcagcact agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc
     6481 gccaatgcct tcaaggaata tggcgaagca gctgtcttga gtatggttat cgatatgcta
     6541 ccgcaattga tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc
     6601 gatacaggaa gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca
     6661 aatttgcttt cttctactca agaaacacta aaagcgacaa caggacttga cttgaaggaa
     6721 atcgtagaga atttgtcgac acgtgggact actagccaag taaatatgta tgaagcgcca
     6781 aaagaggcag aacaaaactc gatgaaggaa gaataaagta gtacacttca ccgttcaatc
     6841 aaaaatggag gaagactttt taaagttctt ctctccattt ttttatttta ttgcaaaaga
     6901 aaattagtcc tcgctatttt tcttttgttt ttggatctta gcttctatct cttgtaaatt
     6961 tgttcttagc aatcgttctt tcttttggag ttcgaccaaa tcttctgata gttcttcctt
     7021 cttttctgtt tcaggaggac gaagaaagaa attgataccg attgcaatga atgtccctat
     7081 aaaagtatct agtacacgtt ggatagcata caaagcggat tcgccttgag ggatgctcaa
     7141 cgcaatcagc aacaacgtag ctattgcgga aatgataccg gaattattgt taataccatc
     7201 tgacacgacg atcacaataa taactagaat aggaagcaag atcaattcta ccaaaaaatc
     7261 attgtgaaaa tagcttttga caaaaaaata aatgatagct aatgtaccac ctaatgtatt
     7321 gccgatgatc ctagactttc caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa
     7381 aacagcagat aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat
     7441 acataaaaaa acagctaagg cacttttgat cgtacgcata cctagtcgaa aacggccaaa
     7501 ttgcatagag attctccttt ttggtctttt tactatcata cctaaacttt tgtctgatag
     7561 ctagtttttc attattcaag tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg
     7621 aatgcgaaca gtcaaaacag tgatcagtgt cttcttttcg atggtcacag cgggtagctt
     7681 atcgctgttg tattggccag ctgcagggat cattgcatta ttaagtgtag gaaataccaa
     7741 gcgctctaca ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag ctacggcaac
     7801 agcactgttc agtttttttg ctgttgggta tactattttt ggcttcagtc tttttttatt
     7861 cctgttcatc ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag taaattcggt
     7921 tttggtcact cattatttag tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc
     7981 gggattaatg gcgattggat tagtatttgc tttgcttgca aatgtttaca tgccagatcg
     8041 tacgaagcag ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa atattttgag
     8101 acaaatggca gaatttcttt tatcagaaaa taaagaagat gtgcagatca aatgcgaaca
     8161 tttactgaca ttcattcgcg aaagccaaga agatgccaga gaacatcaag aaaactactg
     8221 gctcagacag ccgctatact acgaaactta cttctctatg agaagagcac aggccaatgt
     8281 gattaaagac atgctggaaa atctagagag aatccagcaa ccagcttatt atggaaaaca
     8341 tatctatggt ttattgattt atactgcaga aacattttca gagtcaaatg atggcagaca
     8401 aattttaaca cgtattgaag aagtttatgt attgtatcgg caaatgcctc tgccgactag
     8461 tcgccctgaa ttcgaggatc gagcagaatt gttccagttt ttgcagtctt ttaaaagttt
     8521 tattgaaatc aaggcagaat tctcccaaca aaaaatccaa aaatagaggt tgtgaattca
     8581 gctaatattc aggtcaaaaa gctgatttca caacctcttc tttaaacggt gttctcctgt
     8641 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg
     8701 ctccttgtga ctcggagctg aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa
     8761 agctttcctc tgcggtaata agtccagcca aacgaaaaat aggaggagct atttttgttt
     8821 ggctgttctt attacttgat gcagaacagc tttttcacaa ccttttaaaa ttacatattt
     8881 ttccatagaa cttgcatttg atccaagatt tcgatgacat cttttgtcaa gaagagagag
     8941 cgattgggca agtcaccttc aatcatgtgt accatattct ttatttcgta attcattgct
     9001 tcagatgtat tcccgctttc gatgaattcc tttgtaccat ctgtaaataa gatctccgca
     9061 cgatcagcac gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc aacgataccg
     9121 acttttggca tttttgcctg aaaagtcaag ctgacagcag caagttcatt ttctttgtta
     9181 cgcaagatag tgacggattg ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca
     9241 actacttcag gttgagagct caaaaagaaa cgagcgaaag atacagcata cgtaccgata
     9301 tccaacagag ctcctccagc taattctgga ttgaagaacc ggtttttagg gtctggttct
     9361 ttgtaactgc cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc tgtatccatt
     9421 attgagcgta actgctgata aagtggcatg ttgaagattg tcatcgcttc tgctaagatc
     9481 aaattctttt cttctgctaa gcgcatagcg tcagccagtt cttttttatt catcgtgatg
     9541 gctttttcac atagaacgtg tttgcctgct tccaatgctg ccaagatatg gtcgatatgc
     9601 tgtctatttg gtacagcaat gtacacgatg tcgatttcag catcgtctaa catttcttga
     9661 tagcttccgt aagcttttgg aatattgtaa cggtgagcga agttttccgc tttttctgaa
     9721 gttctggaag caactgctgt tagttcgctc gtctcttgat tgaagctttc ggcaaactca
     9781 tgtgcgatgg taccaagacc aacgattccc cattgatatt ttttcatctg aaaacgctcc
     9841 ttcatagttg gattgagttt cttgtctgtc tattattata acaaaaatca cgaagaataa
     9901 agaggagagg acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg
     9961 atcaaaactt aagtagaata gttaaaagga ggggataaac gtgcatcaag tatatattga
    10021 tcttattatg agtgtattat tagaacagtt tgaaactgaa aaaaattttt tagatgaata
    10081 cttgctgatc agtccagaac aatggcaaaa atggaaagat ggcgagaaaa atctgccatc
    10141 tgaagcgatg cagaaagtaa aaaatctttt cactgattat gaatggatgc taacgcaaaa
    10201 gattttgcgt caaacgatta tttttcccga aaaacgaaac gttgccgttg cagagtacaa
    10261 gcaaatcaaa acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct
    10321 gatccctttg aaaaataatc agcaggctgg tagatacctt gacttgaaag taagtattca
    10381 atatgatgaa tggggctatg atgatatttt gaattttcgt ctgccagcag atgttcaaaa
    10441 acagatcgaa ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc tggaagaaac
    10501 ttacgttcat gaggcgaatg gggaacgtga agaacaacga aaggattttt caagtgaata
    10561 aatttttagg atttggagca gttgtgatta tgatcgcagc aatgggctat gttattttta
    10621 gcgatgatga ttcgcatgtt caagccggca atcaagcagt cgaacgaaca agcaccaaaa
    10681 caactgctag taaagaaaag aacagtcaaa caaccagcag caaggtaaag gacaaagaaa
    10741 caaaagatcg tacgtccaaa caattgccag atgttaaaac agacgattgg aagctagttt
    10801 tggttggtcc agatcacaaa ttagaaaaag aaattgatga gtctactcaa ttggcatcat
    10861 taagtaacgg atacttgatc gacaaacgca ttgctccaga atatgaagca tttgccaagg
    10921 ctgctgaagc agctggattt ccattagtga tggtctcagc ttaccggtct gtatcatccc
    10981 aacaacaagt tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag
    11041 aggaggcgac aaagattact aaagaaacga ttacggagcc aggatacagc gaacaccaca
    11101 caggtctggc agtcgatgtc gtcgatcaaa actggtacaa cagttatcct tctactgtat
    11161 tggatgcaag ttacggagat cagcctggag ctaaatggat cgcagataat gctgcgcagt
    11221 atggatttat cgttcgttat ccaaaaggac acgaagatat aactaagatc acatatgaac
    11281 cttggcactt acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa caccaattga
    11341 cgctagaaga atatctgaat gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa
    11401 agtggaaaaa gcaaaagcgc aagaatatcc gttggccagc agtggttgcc ggtgtagcag
    11461 tgatactctt agcctttgtt ttttccttga agaatctgtc ttctcctttt actgataatc
    11521 agcaagaaaa tgaagcgcaa tcccatcagc aatttattga acggcttgtt ccgcatgccc
    11581 aagaactaca agatggttat ggagtgcttc ctagtatcat tttaggacaa gctatattag
    11641 aatcaaattg gggaaaaagt accttggcta gcaaatacaa taatcttttc gggatcaagg
    11701 cttatgggga tcagaagaaa gtaagcctgg aaacaaagga atttgttaac gaagaatgga
    11761 taactatcca aggagacttc aaagtgtatg attcatggga acagtccatg gatgaccata
    11821 cacaactgtt cgtccaagga gtagattgga atcctgcgct gtatgaaaaa gtgattacgg
    11881 ccaccaacta tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg acagaccccg
    11941 gttatgcgca aaaaatcatc caggtcatcg agacgtatca attgaatcag tatgaccgga
    12001 gatagcaaag gagaagtggc tgatggcaga acaatttacg ccagaaatca tgacggaatt
    12061 gagaaaagat atcgtaagag taccggaaat cattttagaa gcaagcggga tcaagatatt
    12121 tggaaaattg atccgttcga tcatctttac tacggatatt gcgatcatac gtaatacaaa
    12181 tgcgaatgca gtaattgctg tgtatccttt tacacctcat ccagcgatta ctaaaagcat
    12241 tattgaggca gctgacattc cagttttctc cggtgtaggt ggtggtttga cacaaggtgc
    12301 tcgttcttcc tacatgagca tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa
    12361 cggtcctacg cctttagaga cagtaaaaga agtttgtcga gtagttgata ttccagttat
    12421 cagtacggta acgtcgaaat atacagaaat agatgaaaag ttagcagctg gtatttcgat
    12481 tatcaatatc agtgcaggta aagaaactgc agaagcagta cgccattttc gtaaaaaata
    12541 tcctgaactt cctatcattg cgacaggtgg tccaacggaa gaaagtattg ctgagaccat
    12601 tcaagcagga gcaaatgcca tcacatatac gccaccaagt agcggagagc tttttagtcg
    12661 taaaatggaa aaatatcggg aaactgagaa aaagaatcat gaataacagg tgagaatcaa
    12721 aagacgagga agagcagata gtggctcttc ctcgtctttt gtactccagt actatttgtt
    12781 cagaattcaa aaaaaagaga aatttttttg tttttttacc tgtaatgtac ataagaagag
    12841 ttaaaacctt ttgttattat tagcattcta acaaaaaaat gtcttgattt attgaagcgc
    12901 tttctataga atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc
    12961 taaattgtcg gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg
    13021 cttgtgcatc gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg
    13081 ttttttaggg aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg
    13141 gcagttgtct gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag
    13201 tgtttttagc gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg
    13261 ttcatcctag gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt
    13321 ttaataaata tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg
    13381 tgcagagaat gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct
    13441 tgacggcttg tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt
    13501 cgcaagtccg acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc
    13561 atgtgacgat cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac
    13621 tagcagcatc agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca
    13681 tcaatcagat tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg
    13741 cagatgaatc ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg
    13801 gggtgaattc gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg
    13861 aaccagcttc aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag
    13921 aattaggaat ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa
    13981 taccacaagt tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg
    14041 gatttgaaaa tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca
    14101 aaggggccca tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg
    14161 cggttaggga tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat
    14221 tgtcaggggc tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag
    14281 atggagaatc tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag
    14341 cccataaaga agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg
    14401 aaattttaaa aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc
    14461 gtgcagatga tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg
    14521 acaacggcga agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca
    14581 atcttttagt tgaaaaagtc atttatgaag aagtaacagc agaaggaagg catgaaaaaa
    14641 gaaaaactaa aacggttctt ggatcaagac aatgtggtcg gttacgtatt tgcttctcca
    14701 ttcatcctag gatttctatt gataacagtg ataccgatga tcttgtcact ttattattcg
    14761 ttcaccgatt atcgtttggg aaatccagtc tcatggatcg gcttggataa ttatataaag
    14821 atgctgagtg atgcccgttt tttgaactcc atccgagtca cttttctcta tgtatttgca
    14881 tctgtacctg tgaaactgat ctttggtcta ttgattgctt atcttttaac gaggaatatc
    14941 aaaggaacga ctttctttcg ttctttgtat tatgtgccta gtttgatcgg tggcagtatt
    15001 tctatcgctc tagtttggcg ggagttgttt tctaaaaacg ggttagtgaa tattttcctg
    15061 gaacgattag gcgcacctgc gctttcctgg tttagcgatc aaaaactggt catgatccct
    15121 cttgttttga tgtctgcttg gcaattcggt tcggcaatga tcatctttgc tgcaggtatc
    15181 aaagagatcc caggctcgta ttatgaagcg gcagagatcg atggagcgaa caaatggcag
    15241 acgttttaca agatcacgtt acctcttctt tcgccgatca ttttgtataa cttggttatg
    15301 cagacaatcg cggcgtttat gacttttacg caggcattta tcattaccca aggtggtccg
    15361 aatgatgcca cgaactttta tgctctttat gtgtataacc aagctttttc ttttaattct
    15421 atggggtatg ctagtgctat ttcgtggatc atgctgttga ttatgggagc tgtcaccttt
    15481 ctgattttca aatcatcaaa aaaatgggtc gttgtgtgaa tgagaggagg aagaaatgaa
    15541 taatacagta acgaaatggt ttcaatacgg ttttattttg ctattaggtc tagtcatgat
    15601 ttatccggtc ttatggatga tcgcaggttc ttttaaaacg gatcaagaga ttatgagtgg
    15661 ttcgctgaat ttgattccag agacttttca atgggaaaat tacgcaaaag gatgggcggg
    15721 gtttgctgga atttcatttt ttactttctt taggaattcg tttgtcatct ctgcagtttc
    15781 gacgataggg acagttctga gctcgacttg tattgcttac gcattatcga gaatcgattt
    15841 caaaggaaaa agattctggt tcgtcgtcat gttagtaacg atgatgattc ctgcacaagt
    15901 gattttgatt ccgcagttta ttatttacaa tcggctgaat ttggttggga cgtatgtgcc
    15961 gctgatttta ccacattttt ttggacaagc atttttcatt taccagatca tgcaattcat
    16021 ggtcagcatc cccaaagagt tagatgaatc agcgattatt gacggatgca gcaagtatag
    16081 tgtctttacc aaaattattt ttcctttgct gaaaccttcg atcatcacga cgatcatcat
    16141 ccagttttat tggaaatggg acgacttcat gggaccattg atttatttaa acaagccgag
    16201 atcttacacg gtatcgattg ccatcaaatt atttgcagac gctggatcta gtacgactta
    16261 cgcgagtatg tttgcgatgt ctacgttgtc gctgctaccg gtttttctga ttttcttatt
    16321 cttcaataaa tatcttgtcc aggggatcag tacaagcggt ctgaagggct gaatatgaaa
    16381 aagccaatag aagagatgtt tctttacaag ctcaactact atgtctgtcg gattttttta
    16441 accagtctta tcagtaatgc tcttttcgct ttagttctag gatgtttgtt cactttagga
    16501 ttagtgctgt ctgctcgtgt tgtctatctg atttattttt tgatgatgta tccttcttat
    16561 tatctatctg tccattactt ggcagaaaaa aaggtatgtt ggaggaaagg gaaaatcaaa
    16621 cggatgtgtt tcatcattac gttgtttcag ctgtttgtca ttatttttgc agttgattgg
    16681 tatttattag tgggaaagat tggagcttgg tatctgattc cttttctatt gtgtctgttg
    16741 ctttttgtat cccatagttt ttttagtacg ctaattgata tctgcaaaga agataaagca
    16801 gatagtcttg tttatttgat caaaaggaat atctggctgt tgtcaaaaaa cgtcaagggg
    16861 tttctgtggt atttatgcct tttgtatgga ctgttctggt gtatacagcc tgatttttta
    16921 ctcactgcat tactgtatcc agggctgcca tttgtcacag tattttgctt aaaagaaaag
    16981 gagaatgact ttaaatgaag aaaaaatatg tacaagtcgg acttggtggg agagcgcgtt
    17041 ttttttatga ggcaattgcc tctacgtatc atcaaacttg tgaactagtc ggtttttgtg
    17101 atgtatctca aacaaggatg gattatgcaa atcggatact tgtgaacaag tatggacaga
    17161 aaaaagtaaa gacatgtatg ccatcacaat ttgagcaact actggatgac gtacggccgg
    17221 ctgaagtcat tattacgtca gtagatcgaa cgcatcatga ttatatcatt cgtgcaatga
    17281 aaaaaggata tgatgttatt tgcgaaaaac ctctgactat cgatgaggaa aaagctcaag
    17341 aaataataac ggtccaaaaa cagacgggaa gaagcctgcg agtcactttt aattatcgtt
    17401 atgcaccgta tcacacgaag ataaaggaac tgatagaaag tgatgtgatt ggtgatgtgt
    17461 tttctatcca cttcgaatgg ctgttaaata cgcaacacgg ggcagattat ttcagaagat
    17521 ggcatcgaaa taagcacaat agcggcgggc ttcttgttca taaatctacc catcattttg
    17581 atctagtgaa tttttggttg aacagccgtc cccaaaaggt atttgcattc ggtggattaa
    17641 acttttatgg gatgcataat gcggagaaac gcggagtaga aatattttac agtcgatgct
    17701 atggaagcga actagctaaa gctgatccat ttgctataga cctgtcagcc aatgaggaat
    17761 tgcgagaaat gtatttagaa gcagaaaaag acagtggata tattcgtgac cgcagtgtat
    17821 tttccgatga tatctctatt gaagatacac taggcgtaac ggtcaaatat gaaactggtg
    17881 cgattttgac gtattcgcta aacgcgtata tgccgtggga aggattcaat gtttctttca
    17941 atggcagcaa aggacggatc gaaatgaaag tgatcgaaaa gtcttacatc aatgcaggtg
    18001 gagataaaaa ggatgaagga gcagccgttc ttcagtccat ccatgtctat ccgatgttcg
    18061 gtgagccata tgaggtaatc gtcgaacaag aagcgggcgg acatggcgga ggagatccgc
    18121 gtttattaga tgatctattt ggtgaagaaa aagaagaaga tccgtggaat cgggcagcaa
    18181 ctcacgtaga tggtattttg tcgattttga caggaatcgc agcaaatcat tctattgctt
    18241 caggaaaagc agtagtgatt gacgagcttg tcagcttaaa agaataagaa tagatttacg
    18301 gaaattactt cccgtagttt ataaaatctt gccttattgc cgataggctg tcttttgaac
    18361 actatttatt cggaaaaagg ggctatgaca tgctttttgt catgccttct ttttatgtgg
    18421 gctgcttttt tagataggag aaaataaaaa taactattta aacccgaaca aaaaggaata
    18481 atgcattaaa aatcattaga ttttacaaaa aaataagtaa accacgttgt cagtttcaga
    18541 aagtagtgtt agactgtttt caatatttga aacaacattt tttattcaaa tatggcaaac
    18601 tgccttcacg tgcaaaacag ttgcaagaaa atgaatttac actattatta gttttacata
    18661 cctacttata tatcgtcata atatggatga cagtttctag ccagtaccgt aaatgctgga
    18721 ctataagtaa aagattggct atttcaccct tcttttctta tgataaagtg gagggtgttt
    18781 ttctttaaca gaaacatagt aggtttttgc acaaaagcgg ttatttcggt attaataaat
    18841 gataaaggag caaataaagt gaaagaatta gttgaacgta ttcaaaaaga cggacgtata
    18901 ttaggcgagg gcgtattaaa agtcgacagc ttcgtcactc accaagtaga ccctgtattg
    18961 atggaacaaa tgggaaaacg gtttgcagaa gtatttaaag aacaaggaat cacgaaagtt
    19021 gtgaccattg aagcttctgg gatcgcacca gctttatttg ctgcaagaga attagatgtt
    19081 ccaatgatct tcgctagaaa agcaaaaagt ttgacgatgg atgaagaatt attgacagct
    19141 tctgtttatt cttttacgaa acaagtgaca agtacgattt ctatttctcg taaattttta
    19201 tcttcagaag acaaagtatt gattatcgac gatttcttgg caaacggaca agcggcaaaa
    19261 ggattgatcg aattatgcca gcaagctgga gcgcaagtag aaggaatcgg gatcgtcatc
    19321 gaaaagtctt tccaagatgg acgtcagtta cttgaagaaa tgggattgag agtcgtttct
    19381 cttgcgcgta ttgcttcctt gacgaatgga caagttgagt ttcttgagga ggacgcataa
    19441 tcgtggaaaa agaaacacaa aatggaaaag ctgcggtttt agggctgcag catctgctag
    19501 cgatgtatgc aggagcagta gcggtgcctc ttttgatcgg tacaggacta ggctttaatc
    19561 aagaacagat gacttatttg atttcaattg atatctttat gtgtggatta gcgacattat
    19621 tgcaattgac agttaaccga tttttcggaa ttggcttgcc tgtcgtttta ggatgtgcga
    19681 tccaagcagt agctccactg atcttgatcg gaaccaatga aggagtagga gctatctacg
    19741 gatctattat cgcttctggg atctttgttg tgttagtttc tggtgtcttt tctaaaatta
    19801 aaaaactatt ccctcctata gtaacaggaa cggttatcac cgttattggt ttgactttga
    19861 ttcctgtagc aatcgagaaa atgggtggag gtaatgcaac agcagctgat tttggtgaca
    19921 aaacaaatct gctacttgca tttgtgacga ttctcctaat tgtaggcgtg caaatgctgg
    19981 caaaagggtt cattcgttca atcgcggtat tgattggttt agtcggagga agcatcttag
    20041 ctgctttcat gggaatgatt gatctgagcg cgattggaga tgcgccgttg ttccatgttc
    20101 cacaaccctt ttattttggt aaaccaactt tcgacgtatg gtcgatttta ctaatgatca
    20161 tcatttccat ggtcagcatg gtagaatcaa caggtgtata ttttgcactt ggtgacatta
    20221 ctgggaaaaa tgttggagaa gaagaattga aaaagggata tcgtgcagaa ggacttgccg
    20281 ttatcttagg cggtattttc aatacattcc cttatactgg cttttctcaa aatgtcggat
    20341 tggttcaact ttcgggaatc aaaacaagaa aacccatcta tttctctgca ttcttcttga
    20401 ttgttttagg attattccct aaaatcggtg cgttagcaca aatcatccct gagccggtac
    20461 ttggtggagg aatgttagtc atgttcggga tggtggcagt ccaaggaatc cgtatgctgt
    20521 tggaagttga tttcagcaat gataaaaatc tattgattgt tgctgtttct gttggatttg
    20581 ggctgggttt caatgttacg ccgacattat tcagtcaaat gccgcaaaca ttgcaaatgt
    20641 tcactggaaa tggcatcgta atgagtagca ttacggctat catattgaat ttgatcttta
    20701 atggattgaa aaaagatgaa gaaatattgg aagaagcctt caagaagtaa tagagcaaaa
    20761 gaaaggttgt gaaatcagcg ttttgacctg attgttagag aaacaaacgg aaaaatagtt
    20821 cctcgtattt tgccgtttgt ttgagctaat accgagggtc agctgattga acaccgttta
    20881 tccataaaaa ggaatgtgac ctaacttttg tcacattcct ttttttcatc tttaataaac
    20941 acgaacaata aaatgttttt ctttgttaac attcgaaatt tctttgacat caaaaaaagt
    21001 gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa caatgacagc aaaaatctct
    21061 gtgattatgg ggagtacttc agattgggaa acaatgaaag aagcatgtca aatattggat
    21121 gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc agatttgatg
    21181 tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg
    21241 ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta
    21301 ccggtccaat cacgaacatt aaatggtttg gattcgcttc tttcgattgt ccaaatgcct
    21361 ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa tgctggacta
    21421 cttgcgatac agatgctttc catgtatgat ttagaattgg aggctaaatt ggctgaaaga
    21481 cgttcaatgt tggctaaaac tgttatagaa agtagtgatc aacttggtta aacctttgtt
    21541 gcctggttca acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag
    21601 tgcaaaagaa atgggcttta aagtaggtgt gctggatcct gtgagcgatt gtccagccgc
    21661 acaagttgca gattggcaca ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct
    21721 tgccagaaga acagatgtca ttacctatga attcgaaaac gtcagtgtag aggcgttaaa
    21781 tgcgatttta cccatgtcat ttattcctca agggacagac ttgcttgcga ttacgcaaga
    21841 ccgcctgtta gaaaaatctt ttttggaaac gaataacatc gtgattgctc cttatgcaac
    21901 gatcgtcagt ccgacggata tccaagatgc gattgacggg atcggctatc cttgtgtttt
    21961 gaaaaccact cgaggcggct atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga
    22021 tctagctcct gccatggacc ttttaagaga aggaacctgt gttttagaag catggatccc
    22081 gtttgaaaaa gagatttcga tcatggtggc aggaaatggt caaggagatt tcacgacttt
    22141 tccggtcgtg gaaaacatcc accacaacaa tattcttcat gaaacgattg ctccagccgc
    22201 gattgatcag gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg cagaagcagt
    22261 gagcctttca ggtgttttag gggtagaaat gtttttaaca aaaacaggcg ggctatatgt
    22321 gaatgaatta gctcctcgtc ctcacaattc tggacattat tctattgaag cttgttctat
    22381 gagtcagttc gatgcccata ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg
    22441 tttgataagc aatgcagtaa tggtcaatgt tttgggagaa caactgctgg aaagctatcg
    22501 cttgattgag aataagccag actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa
    22561 aggtcgcaag atgggacata tcactatctt gacagaagac atccagcaaa cgctggaaga
    22621 aatcaaacaa acgaatattt gggattagag gagaaaaaac atgttagaac gttatacacg
    22681 ccctgaaatg gggaatatct ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga
    22741 gattttagca gatgaagctt gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa
    22801 aattcgtgag catgcatcat ttgatatcga tcggatcttg gaaatcgaaa aattaacacg
    22861 ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg
    22921 ggtccattat ggtctaacaa gtacggatgt cgtagatact gcttatggct atctcatcaa
    22981 gcaagcaaat gatattttac gggaagactt gaaacgtttt acggaaatca ttggcgaaaa
    23041 agcaaaagaa cacaaatata cagtgatgat gggtcggaca cacggtgtcc atgctgaacc
    23101 aactactttt ggattgaaac ttgccacatg gtattcagaa atgaaacgga acatcgaacg
    23161 atttgaacat gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt
    23221 cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa caattgggga tccgtccaca
    23281 agaaatctct actcaagttc tccctcggga tctacatgct gaatatttct cagcgatggc
    23341 gcttattgct acaagtattg aacgttttgc aacagagata cgcggattgc aaaaatccga
    23401 aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca
    23461 caaaaggaat ccgatcggtt cagaaaatat ggctggtcta gccagagtgg tgcgcggaca
    23521 catggtcact gcctatgaaa atgtcacatt atggcatgaa agagatattt ctcattcttc
    23581 agcagaacga atcatcttgc ctgataccac gattttattg aattacatgt taaatcgttt
    23641 tggcaatatc gttaaaaatc taacggtctt tcctgaaaac atgaagcgga atatgtctgc
    23701 aacatttggt ttgatttata gccagcgcgt tatgctgaaa ttgatcgata agggaatgac
    23761 aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt
    23821 gcagttccgc ccgctgttag aagaagatcc aaccatcaca tcggtattat ctaaagaaga
    23881 aatagacgat gcctttgatt atcatcatca tttgaaaaac gttgacgtga tattcaaacg
    23941 agtaggtttg gaataaagaa gagtgagttg caaaattcaa ttaaaataga atagcaaaaa
    24001 taaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct
    24061 tatcaataat aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata
    24121 caaaaaatag aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg
    24181 gagatgttgc agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat
    24241 ctggatacga taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc
    24301 gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt
    24361 tgctcggcgg atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc
    24421 tttgatcgac agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa
    24481 aaaaagctga ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat
    24541 ccaaattttt agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat
    24601 ggtataagat tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg
    24661 tacatatttt tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac
    24721 aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa
    24781 gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt
    24841 tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt
    24901 ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga
    24961 ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta
    25021 caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt
    25081 ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat
    25141 gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaaa
    25201 ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa
    25261 ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat
    25321 ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt
    25381 acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc
    25441 tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt
    25501 atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag
    25561 taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat
    25621 actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt
    25681 gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg
    25741 aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat
    25801 atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag
    25861 tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat
    25921 ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct
    25981 gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga
    26041 cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca
    26101 ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga aataatctat
    26161 cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta
    26221 aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac
    26281 attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt
    26341 gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa
    26401 aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc
    26461 aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa
    26521 gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg
    26581 agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga
    26641 cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta
    26701 gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga
    26761 gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt
    26821 gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta
    26881 gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc
    26941 ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta
    27001 ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc
    27061 atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa
    27121 gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg
    27181 gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata
    27241 ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa
    27301 gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca
    27361 ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga
    27421 gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt
    27481 aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg
    27541 ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct
    27601 cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa
    27661 gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact
    27721 tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg
    27781 agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg
    27841 tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt
    27901 gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa
    27961 ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg
    28021 ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca
    28081 atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact
    28141 actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat
    28201 tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac
    28261 tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga
    28321 ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca
    28381 gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt
    28441 gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa
    28501 gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa
    28561 atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag
    28621 gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg
    28681 tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc
    28741 gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag
    28801 atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag
    28861 aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc
    28921 agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga
    28981 tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact
    29041 ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata
    29101 aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa
    29161 cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga
    29221 aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt
    29281 ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg
    29341 attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca
    29401 gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga
    29461 atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt
    29521 tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa
    29581 aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg
    29641 ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg
    29701 atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag
    29761 gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg
    29821 ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca
    29881 aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga
    29941 tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt
    30001 atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat
    30061 cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca
    30121 aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc
    30181 aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat
    30241 ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg
    30301 aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg
    30361 atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta
    30421 gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga
    30481 tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac
    30541 tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta
    30601 ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt
    30661 cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta
    30721 tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa
    30781 atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat
    30841 atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca
    30901 caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt
    30961 gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca
    31021 gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt
    31081 tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa
    31141 cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt
    31201 atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag
    31261 catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag
    31321 aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga
    31381 tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt
    31441 atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg
    31501 gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg
    31561 accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg
    31621 acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag
    31681 tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt
    31741 atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat
    31801 catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat
    31861 cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa
    31921 aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac
    31981 ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat
    32041 tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag
    32101 tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga
    32161 ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca
    32221 aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata
    32281 cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa
    32341 acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct
    32401 tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa
    32461 taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt
    32521 agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc
    32581 gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa
    32641 tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat
    32701 tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt
    32761 actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt
    32821 aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt
    32881 cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc
    32941 tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat
    33001 cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga
    33061 gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga
    33121 atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca
    33181 agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca
    33241 ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg
    33301 tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc
    33361 gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga
    33421 tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc
    33481 tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt
    33541 cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat
    33601 ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc
    33661 agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg
    33721 gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt
    33781 gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg
    33841 agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc
    33901 gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc
    33961 ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt
    34021 aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc
    34081 gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga
    34141 tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga
    34201 tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt
    34261 tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt
    34321 tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt
    34381 tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca
    34441 gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga
    34501 tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt
    34561 ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga
    34621 gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat
    34681 taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg
    34741 cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc
    34801 atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa
    34861 catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga
    34921 tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga
    34981 aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg
    35041 aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca
    35101 gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag
    35161 ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca
    35221 atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc
    35281 atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta
    35341 tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc
    35401 aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag
    35461 tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta
    35521 atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa
    35581 gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg
    35641 ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg
    35701 gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga
    35761 gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt
    35821 tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg
    35881 attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta
    35941 ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga
    36001 tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc
    36061 cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg
    36121 taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt
    36181 tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat
    36241 aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac
    36301 ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca
    36361 gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta
    36421 agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg
    36481 attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat
    36541 tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa
    36601 tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc
    36661 tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg
    36721 acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat
    36781 tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa
    36841 tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa
    36901 tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta
    36961 agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat
    37021 ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc
    37081 aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata
    37141 attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa
    37201 gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg
    37261 ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag
    37321 tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc
    37381 gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga
    37441 atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat
    37501 cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgcgg taatacgaat
    37561 aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg
    37621 caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc
    37681 aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc
    37741 tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag
    37801 tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc
    37861 atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact
    37921 ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc
    37981 cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg
    38041 aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc
    38101 ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag
    38161 aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt
    38221 gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt
    38281 gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat
    38341 attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc
    38401 taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct
    38461 aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa
    38521 gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg
    38581 gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag
    38641 tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt
    38701 tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag
    38761 ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc
    38821 ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag
    38881 gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt
    38941 gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg
    39001 cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata
    39061 aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac
    39121 cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta
    39181 gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg
    39241 agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt
    39301 gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg
    39361 tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc
    39421 ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa
    39481 tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat
    39541 tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg
    39601 attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta
    39661 gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa
    39721 caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt
    39781 actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca
    39841 agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca
    39901 taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg
    39961 gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc
    40021 aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt
    40081 aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt
    40141 ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga
    40201 aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc
    40261 ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa
    40321 ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt
    40381 tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa
    40441 agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc
    40501 actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt
    40561 ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc
    40621 tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga
    40681 tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt
    40741 ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc
    40801 tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat
    40861 cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt
    40921 caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac
    40981 agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt attataaaga
    41041 atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag
    41101 aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct
    41161 ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg
    41221 tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca
    41281 tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg
    41341 aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat
    41401 ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat
    41461 tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac
    41521 ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa
    41581 gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa
    41641 gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc
    41701 atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta
    41761 cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt
    41821 cgcgggatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga
    41881 gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc
    41941 ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg
    42001 atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag
    42061 atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc
    42121 attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg
    42181 atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta
    42241 ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa
    42301 acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt
    42361 ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg
    42421 atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc
    42481 aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc
    42541 tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa
    42601 gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct
    42661 tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg
    42721 ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg
    42781 tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt
    42841 actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc
    42901 agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc
    42961 gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt
    43021 ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt
    43081 tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt
    43141 ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt
    43201 gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag
    43261 aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc
    43321 ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta
    43381 tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa
    43441 aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc
    43501 agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc
    43561 tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc
    43621 gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat
    43681 tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat
    43741 gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt
    43801 agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta
    43861 ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca
    43921 ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat
    43981 tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa
    44041 agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg
    44101 taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata
    44161 gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag
    44221 tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg
    44281 gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa
    44341 tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct
    44401 ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc
    44461 atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa
    44521 cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg
    44581 gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga
    44641 caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc
    44701 gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc
    44761 gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg
    44821 gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat
    44881 tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat
    44941 ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg
    45001 gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt
    45061 ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc
    45121 tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg
    45181 aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa
    45241 gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc
    45301 ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga
    45361 gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa
    45421 cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg
    45481 tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac
    45541 gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc
    45601 aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca
    45661 aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga
    45721 gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt
    45781 agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg
    45841 gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg
    45901 cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc
    45961 tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt
    46021 atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca
    46081 taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt
    46141 gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat
    46201 tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat
    46261 acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa
    46321 aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc
    46381 gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt
    46441 taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg
    46501 actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg
    46561 ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt
    46621 agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat
    46681 cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa
    46741 aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac
    46801 agcttggatt taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga
    46861 aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt
    46921 gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt
    46981 agaaaataga aaaaatgcag atgaaaaata aaacgctttc cttattgtga ttttattcac
    47041 taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta
    47101 gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca
    47161 gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg
    47221 tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca
    47281 atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt
    47341 tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc
    47401 gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac
    47461 caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg
    47521 ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc
    47581 aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag
    47641 ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag
    47701 gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat
    47761 cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat
    47821 tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa
    47881 aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg
    47941 atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg
    48001 gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg
    48061 atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga
    48121 tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag
    48181 tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa
    48241 aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag
    48301 aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat
    48361 ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt
    48421 tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa
    48481 gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag
    48541 tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat
    48601 tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg
    48661 cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga
    48721 attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat
    48781 tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc
    48841 ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga
    48901 agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat
    48961 tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga
    49021 aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat
    49081 cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc
    49141 gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc
    49201 cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt
    49261 ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga
    49321 aaagaaggga cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg
    49381 aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga
    49441 tgatacatta ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac
    49501 aggaacggtt aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt
    49561 agtagaaatc gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga
    49621 atcccaaact ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc
    49681 gtcaagcgaa ggagtcttcc aatttaagtt accggatatc ggtgaaggaa tcgcagaagg
    49741 tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact
    49801 agaagttcaa aatgacaaat cagtagaaga aattccgtca cctgtcacag ggacagttaa
    49861 aaacgtgatt gttccagaag gcactgtagc aaatgttggg gatgtattag tagaaatcga
    49921 tgctcctgga cacaatagtg caccgtcaac tagtgcgcca tcagctgaag caccaaaaga
    49981 aaaagtggaa acatcaggtt cagcaagtgt cgttgaagct gccgatccaa ataaacgtgt
    50041 attggctatg ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata tctcacaagt
    50101 aactgcaact ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact tcttggcagg
    50161 tggtccaagc agtgcaccag cgaaatcaga agcgccagaa gcggcagcac cgaaagaagc
    50221 agctcctgca gcagaaagca aaccagctgc accagcaaaa ccattcaagt ccaacttagg
    50281 agacttggaa gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta aagcaatggt
    50341 caacagtaag catacagctc cacacgtgac attgcatgat gaagtagaag tgtctaaact
    50401 atgggataat cggaaacgat ttaaagaagt tgctgctgca aatggtacga aacttacttt
    50461 cttgccatat gtcgtgaaag ctttaacagc gactgttaag aaatacccag tattgaatgc
    50521 atctatcgac gatgcaaacc aagaaatcgt ttataaacat tactacaata tcggtattgc
    50581 aactgatacg gatcacggac tatatgtacc aaatgtgaaa gatgcagacc gcaaaggaat
    50641 gtttgctatt gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg gcaaactatc
    50701 tgcagaagat atgcgcaatg gaacaattac gatcagcaat atcggatctg taggtggcgg
    50761 ttggtttaca ccagtcatca actatccaga agtagctatt ttaggtgtag gaacgattgc
    50821 acaacaacca atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga tgaaactgtc
    50881 attaagtttc gatcaccgta tcgtagatgg agcaactgcg caacaagcaa tgaataatat
    50941 caaacgtctt ttagctgatc cagagctatt aatgatggaa ggatgataaa caaatggtag
    51001 ttggagattt tgctgtagaa ttagatacag ttgtaattgg tgcgggacct ggtggctatg
    51061 tagctgccat ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa agagagtata
    51121 ttggcggtgt ttgtttgaat gtcggctgta ttccttcaaa agcactgata gctgcaggac
    51181 accattatca agagtcacta gattctgaaa tgtttggagt aactagtgaa aacgtcaaac
    51241 tagattttgc aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg ttgacttctg
    51301 gtgttggttt cctattaaaa aaacataagg ttgaaacaat cgaaggagaa gcgttcttcg
    51361 tagatgatca cacactacgc gtcattcatc cagattcagc tcaaacttat tcgttcaata
    51421 acgcaatcat cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa ttcggaggac
    51481 gtgtgttaga ttctactggc ggattagcat tgaaagaagt acctaagaaa tttgtgatca
    51541 tcggtggcgg tgtgattggt gccgaattgg gcggagcgta cgcaaactta ggagcagaag
    51601 taacgattct tgaaggctca ccacaaattt taccaactta cgaaaaagat ctagtaaaat
    51661 tagtagaaga tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca atggcgaaag
    51721 aagcagtcga taacggtgac agcgtgacag tgaaatatgc agtggacggg aaagaagaat
    51781 ccgttactgc tgattatgtg atggtcactg taggtcgtcg tccgaataca gatgatatgg
    51841 gcttagaaca agcgggtgtc gaagtaggag aacgaggatt gatcacggtg gataaacaag
    51901 gacgtacgaa cgtacctaac atctatgcca tcggtgatat cgttccaggg gcggcattag
    51961 cacacaaagc cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc aaaaaagtag
    52021 ctgtagatta taaagcaatg ccggcagtag ccttcactga tcctgaattg gcttctgtag
    52081 gtatgacgat caaagaagca aaagatgctg gtatcgaagc aaaagcatat aaattcccat
    52141 tttcagggaa cggtcgggca ttatcgcttg gtaaaacaga aggattcatc cgtctagtta
    52201 cgacgatcga agataacgtg cttattggcg cgcaaatcgg tggagtcggc gcaagtgata
    52261 tggtctctga attagcttta gcaatcgaat caggaatgaa tgctgaagat atcgccttga
    52321 cgattcatcc tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa ttagcattag
    52381 gtttaccaat ccatatttga tttgaataat acaggcgaaa cgcaactgaa aaatcagttg
    52441 cgttttgttt tttagaatta tcaataatat tattatgtaa acttaaaaat atgacaactt
    52501 cttggtttat taccacttcc tatgctacac tgaagaggaa tatgaaatag gagagttgaa
    52561 gatgcaagaa tatcagtatc cacttgattt agattggaca acagacgaaa tggtcattgt
    52621 aatgaagatg tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca atgaagcttt
    52681 tttgaagact tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg aaaaacgatt
    52741 gggacgggaa tttgaaaaag cttccggtta ttctttatat cgtacagtgc agcaagcaaa
    52801 aaaaaatcca aacaaaactt tgagaatgaa cgggtgagat aaatggaaca gatgattgat
    52861 catatcaagt cctggcttga agaagctggt gagaaaatca aatcttctca acaaacatta
    52921 acaatctcgc aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg aaacatccag
    52981 aattttctaa tcgaaaaaat ccattccttt gatccagagg cgaaaatatt ggcagaagaa
    53041 gaagggtgta atcaattatc ggatctcgat ggaagagtct ttatcatcga tcctattgat
    53101 ggcaccttga attttgtgat tgaaggggaa aatttttgta ttatgctcgc tgtttatgaa
    53161 tccggcgttg gaaagcttgg tttcatttat gacgtgatga gaaatgaact tttttggggt
    53221 ggaaaaggaa taggcgtgta taaaaatgat gagccattgc ctagaccagc aaataaatcc
    53281 ttatcggaag gcttacttgg tgtgaatagt tatcttttcg gtcataataa atatggaatc
    53341 cgagaaattg cggaaagcag catggggaca cgtatgtacg gatgcgctgg tcttgaacta
    53401 gtggcaatct tgaaaggaaa ccacattgct tatatttcaa accttagccc ttgggattat
    53461 gcagcgggaa atgtcttatt ggaagagttt ggtatggtaa caagcggtct ttttggtact
    53521 cctttgacat ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc atatgcagaa
    53581 atcgcagacc ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc acaaaaatga
    53641 taaaaacgag caaaagatta attttctcga aaaaatgaaa acattttact atatttcatg
    53701 aaaaaagtct gctataatta gcagtcgagt aaaatttgcc ctgtcataaa gaaccaataa
    53761 aaaaggagtt agttacattg aattatagaa acgatatccg taacgtcgca attattgccc
    53821 acgttgacca cggaaaaaca acattagttg atgaactatt aaaacaatca gatacattag
    53881 acgcacatac gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa aaagaacgtg
    53941 gtatcacgat tttagccaaa aacacagctg tagactataa aggtattcgc gtaaacatca
    54001 tggatactcc aggacacgcg gacttcggtg gcgaagtaga acggatcatg aaaatggttg
    54061 acggcgttgt gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca cgttttgtat
    54121 tgaaaaaagc actggaacaa catatcacac caatcgttgt cgtaaacaag attgacaaac
    54181 cttctgctcg tcctgagcat gtcgtagatg aagttcttga actattcatc gaattaggag
    54241 cagatgatga tcagttagac tttcctgtga tttatgcatc tgcactaaat ggtacgtcaa
    54301 gcttgtcaga tgatccagca gatcaagaac caaccatggc acctattttt gatacaatta
    54361 ttgaaaaaat ccctgctcct gtcgataaca gcgacgaacc attacaattc caagtttcat
    54421 tacttgacta caacgattat gtaggtcgta tcggaatcgg acgtgtattt agaggaacaa
    54481 tcaaagtagg agaccaagta gcattaatta aattagacgg aacagttaag aaattccgtg
    54541 taacgaaatt atttggtttc tttggactaa aacgtctaga gattcaagaa gcaaaagctg
    54601 gagacttgat tgctgtctca ggtatggaag atatctttgt cggtgaaaca gttactcctg
    54661 tggatcatca agatgcgtta cctatccttc acattgacga accaacattg caaatgactt
    54721 tcttagttaa taattcacca tttgccggtc gagaaggtaa attcgtaacc gctcgtaaga
    54781 ttgaagaacg tttgatggca gaacttcaaa cagatgtatc cttacgagtt gaaccaacaa
    54841 actctccaga tgcatggaca gtttctggtc gtggagaatt gcatttgtct atcttgattg
    54901 aaaacatgcg ccgtgaggga tacgaattgc aagtatctcg tccagaagtt attgaaaaag
    54961 aaatcgacgg tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca ccagaagaat
    55021 atatgggtag tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa gatatggtcc
    55081 atacaggaaa cggacaaatc cgtttgacat tcttaacacc tgcccgcggt ttgattggtt
    55141 attcaactga attcctttca atgacacgtg gttacggaat catgaaccat acttttgacc
    55201 aatatctgcc aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg cttgtttcga
    55261 tcgatacagg aaaagcaacg acttattcta tcatgagtat cgaagaacgc ggtacagtct
    55321 tcgtagaacc aggtacagaa gtatatgaag gaatgattat cggggaaaac agccgtgata
    55381 atgatttgac tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg tctgctacta
    55441 aagaccaaac atctgtcatc aaaaaaccaa aacaattgac gttggaagaa tctttggaat
    55501 tcttgaatga tgatgaatat tgcgaagtaa caccagaaag cattcgcttg agaaaacaaa
    55561 ttttagagaa aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct gtaaaaggat
    55621 gaagagagaa accagcttgt tataaaaaat agctggtttc tttttttgtc caactctttt
    55681 ctgtggagaa gtcatctttg tcaatggaca taaattcaga aaatacgccg aaagacgttt
    55741 aaaagacttt taaaaaactt atttctagag agtttttgtt tcataataag ataatgtttc
    55801 acgaaaggag atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa gagaaaggag
    55861 cgggaagatg aacgacttat taaaacaata ctttggttat gatgagttta gacctggtca
    55921 aaaagaaatt atccagaaag tcattgatca ggaaaatgta ttaggaatca tgcctaccgg
    55981 aagtgggaaa tcaatctgtt accagcttcc agctttacta ttagatgggc ttactgtggt
    56041 tgtgtcccct ttaatctctt tgatgaagga tcaggtggac gcggcgaatc aattaggtat
    56101 acccgccact tttatcaata gctcgttaga tggttacgaa acagctagac gcttccaaga
    56161 aatcgataga cagcagtacc gtttacttta tatcgctcct gaacgtttta tcatgccgga
    56221 ttttatccaa gcaatgaagc gatggaacgt ccgtatgatc gctattgacg aggcacattg
    56281 tatctctcag tgggggcatg atttccgccc aagttattta cagatggcga accagctaga
    56341 tcaactgcca aatcgtcctg taattgttgc tttgacagct acagcgactg tgcaagtagc
    56401 tgctgacata aaaagacttt taaaaattcc agaaaacaac catattcaga ctggttttga
    56461 acgagaaaat ctacgatttc aagtaattaa agatcaaaaa aaagaacaat atctaatcga
    56521 atatttaaag atcaacaaaa atcaatctgg tatcatttat gcagcaacga gaaaagaagt
    56581 agaccgtctc tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat atcatggtgg
    56641 tctaaatgag aatgagcgaa cagaaatgca agaagccttt ttatacgatc gcttgcagct
    56701 gattgtagcg acaaatgcat ttggtatggg tataaataaa agcaatgtcc gttttgttat
    56761 ccattatcaa attcctggct ctctagaagc atactatcag gaagctggtc gggcgggaag
    56821 agatggtctt tccagtgaag ccatcctgct tttctctcct caagatatcc aagtccaaaa
    56881 gttttttatc cagcagtccc aaagagaaga agggcaaaag caaaaagaat atgaaaaatt
    56941 aaaagcgatg acagaatacg tgtatattga gtcctgtctt cagcaataca tcctaaacta
    57001 ttttggcgag acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg acagagaaat
    57061 agtagaagta acgacagaag cacagatggt tctttcttgc ttaaaacgta tgggtgagaa
    57121 ctatggtaaa caaatgctga tgaaagtctt agcaggatcg aaagaacaaa agctgcaagc
    57181 tttaggtttt ggacatttat ctacctatgg actgatgaaa aaccaatcgc aaaaagaaac
    57241 gatgcagtta attgaatatt tgatatcgaa tggctatctt ctgaccatca atggtgagta
    57301 tcctgttttg aaagtaacag aaagagggat acaagtattg aaaggacaag aatctgttta
    57361 tcgaaaggaa cccaagaaag tccagcagct atcggatgaa gaaaccgata cactatttga
    57421 agtgctgcgg gaactgcgaa cggatctagc atcagaagca ggcgttcccc cgtatgtcgt
    57481 attttcagat tccacattga aagaaatgag ccgaatacgt ccatcaagca gattggagat
    57541 gcttcagata aaaggagttg gccaaagcaa gcttgataaa tatggcgaag cctttttatc
    57601 tcggataaaa aatgcggact ctaacgttct ggataagtag aaagaacgaa tacgtaaaaa
    57661 tgaataaaaa caactggtta aatctgtttt ttttgctcct tatgaaaatc aaatatgtta
    57721 taattaagga attagaatgg gaggtaagca cagatggatt ctttgtcgaa aaaacttgcg
    57781 atccaagtat taaatgatga agcagaacgg atcaaacgat taatccgaaa tcaaaacaac
    57841 agtctctgta tctcacaatg caaagccttc gaagaagtgg tagatacaca gatgtacggc
    57901 ttttcccggc aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa cgagggccag
    57961 ctgatgttaa gtgagctaga acgagaattg aatcagttat atagtgaggt ttaccaagaa
    58021 atttatgata aaaaagaagt tggcaaggag gaatagactt gcctaagaaa gtgaaaaaaa
    58081 ggcatctatt agactatagc atcttgattc cctatttaat tttatgtgta ttgggattga
    58141 tcatggtata tagttctacg tcgtatctat tattagaaaa cggacaaaat ccgtccgcat
    58201 cagtcattaa ccagagtatt ttttgggtgc ttagcttgat tgctatcgca ctactttata
    58261 aaatgaaaac agatgttttg aaaaaccagc gtttgatcat ggcagccatc gcagttttga
    58321 cgattttgct gctgattgtt gtgttttttg gtaaggagat caatggggcg aaaggttggc
    58381 tgcaaattgc aggattctcg atccaacctg ccgaatacct aaaaatcatt tctatctggt
    58441 acctgtctct gactttatct aaaagacaaa acagtgtaca aaaagatttt cttgggactg
    58501 tgaaacgtcc acttgcaatg gttatcggac ttactgcatt agtcgctatt cttcccgatt
    58561 ttggtaatgc ggcagttatc tttttgatta ttttggttct gcttttagcc agcggagtca
    58621 attatgtgta tactttgatt gtaggagtag gaggcttttg tctaagtact tttacgatct
    58681 ggctgatcaa tatcacaaac ggaaagatct tacccggacg tttacagtat atctataatc
    58741 gatttgctat ttaccaaaat cctttctcag acgaattaaa taaagggcat caactagtca
    58801 acggctacta tgccatgttc aacggaggac tatttggcag gggattaggt aacagtatcc
    58861 agaaaaaagg atttttacaa gaagcacaaa ccgattttat ttatgcaatc gtagtggaag
    58921 aactgggagt gattatgggg attttgatat tagcgttgct attctttatg attgtgcgca
    58981 ttgtccttgt aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc attgggatcg
    59041 gcgcaatgtt tttgatccaa gtatttgtca atcttggagg aattactggc gttattcctt
    59101 tgacagggat cacgttccca tttttaagtc aaggaggctc cagtttgctg atgctttcga
    59161 tttgtgtcgg ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc ttaggtttat
    59221 aataaaaaaa gatgtaggag tggttcgatg aaaaaagttt tagttgctaa caggggtgaa
    59281 atcgctgtac gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt tggaatctat
    59341 gcagaagaag atgaatattc cgtacaccgt ttcaaagcgg atgaagcata tttagtagga
    59401 caaggaaaaa aaccgattga tgcgtatcta gatattgaag ggatcatttc tatcgcgaaa
    59461 gaatgcggag ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa cttgaatttt
    59521 gcgcaacggt gcgaagaaga aggcatcatt tttgttggac ccaaactgca tcatttagat
    59581 atttttggag ataaaatcaa agcaaaagct gcagctatcg aagcggggat tgcttcgatt
    59641 ccaggcacag atggaccaat cgcttctata gacgatgcct tggagtttgc gaaacaatac
    59701 ggttatccga ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat gcgtgtagct
    59761 cacgatgaaa agagtgcaag agagggctac gaacgtgcga aaagcgaagc aaaagcagcc
    59821 tttggtagtg atgaagtgta tgttgaaaaa tacatcgcta atcctaagca tatcgaagta
    59881 cagattctcg gggatacgca tggaaacgtc atccatcttt ttgaaagaga ttgttctgtt
    59941 cagcgccgtc accaaaaagt agtagaagta gcgccttgtg tctctatgaa tgaacagcaa
    60001 cgtcaaaaaa tctgccaggc agctgtccaa ttgatgaaat atgtcgggta tgtcaacgca
    60061 ggtacagtag aatttcttgt tgaaggagat gatttttact ttatcgaagt caaccctcgc
    60121 gtccaagtgg aacatacgat cacagaaatg attacagata ttgatatcgt aacaacgcaa
    60181 ctgttgattg ctcaaggttt ggatctgcat aaagaaatcg gattgcctca gcaagaagga
    60241 attaaattaa atggttcagc cattcaatgc cgaatcacta cagaagaccc gttgaataac
    60301 tttttaccag acacagggaa aatcgatacg tatcgttcac ctggtggttt tggcgtacgt
    60361 ctggatgtcg gaaatgctta cgcgggctat gtagtgacac cgtattttga ttcattgcta
    60421 gtaaaagtat gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat ggaacgttgc
    60481 ttgaaagaat ttagaatccg tggagtgaaa acaaacattc catttatgct caatgtcatc
    60541 acacatcctg aattccaatc aggaaatgca aaaacaacct tcattgatag tacagcaaca
    60601 ttatttgaat ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata tatcggtgaa
    60661 attactgtga atggttttcc aggtatcgaa agtggcgaaa aaccatttta tgaagaacca
    60721 cgtatgccta aagatctaat cacaagaact gattacgtaa cggctaagaa tgtattagat
    60781 gcgaagggtg ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt gttgttgacg
    60841 gatacgacat ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg gacaaaggat
    60901 ttcaagcaga ttgcacgttt gactggagaa ggtcttcctg aactcttttc aagtgagatg
    60961 tgggggggag caacatttga tgtggcttac cgctttttga atgaagatcc ttggcaacgg
    61021 ttgagaaaaa ttcgttcttt aatgcctaat acgttgctgc aaatgctttt cagaggttca
    61081 aatgccgttg ggtattctaa ttatcctgac aatgtattgg tggaatttgt caaagaagca
    61141 gcagcgcaag gaatcgatgt atttcgaatc tttgatagtt tgaactggac tccacaaatg
    61201 gaaaaaagta tccaagctgt acgggatact ggtaaaatcg cagaggcagc tatttgctat
    61261 actggcgata tcaacgatcc aagtcgggca aaatacaatg ttcagtatta taaagacatg
    61321 gcaaaggaac tagaacaact gggtgcgcat atcattgcaa tcaaagatat ggctgggctg
    61381 cttaaaccgc aggcagctta tcgtttgatt agtgaattga aagaaacgac agatctgcca
    61441 atccatctgc atactcatga taccagcggg aatggaatca tcacttattc tgctgcttca
    61501 aaagcaggag tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa tactagtcag
    61561 cctagcatga gcagcttgta ttatgcactg gtcaatggtc cacggctgcc agaaatcacg
    61621 attgaaaatg cacagaaatt aaaccattat tgggaagatg tgcgcatgta ttacaaaccg
    61681 tttgaaaacg gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat gccaggagga
    61741 cagtattcga atctacagca acaggcaaaa gctgtcggat taggtcatcg ctgggatgaa
    61801 atcaaacaaa tgtatcatac tgtcaacttg atgtttggcg atatcgttaa agtaactcca
    61861 tcatctaaag tagttggaga tatggcacta tttatggtcc aaaatgatct tacagaagaa
    61921 gatatttatg aaaaaggcga aacactaagt ttccctgaat ctgttgtgac tttcttccaa
    61981 ggagaactag ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat tattcttaaa
    62041 ggcagacctg cattgaatga acgtccaggt cttttggctg aatcagtcga cttcaacgaa
    62101 gtaaaaaaag aattggcaga aaaaattggc tatgaaccaa aacaagacga agtattaagc
    62161 tatttgatgt atccgcaagt ctttttggac tatcaaaaag catataatca atttggcgat
    62221 gttacattac tggacacacc aacatttttc caaggtatac gtttgggtga aacaatcaat
    62281 gtacaaatcg aacgcggaaa aacgcttatc attcgtttag atgaaatcgg agaacccgat
    62341 atcgaaggga atcgcgtact gttcttcaat ttgaatggac aaagacgtga aatcactatc
    62401 aaagataatt cgatcatcag tacggtccaa acccgattga aagctgaacc gacaaaccga
    62461 gaacaaattg gagcaacgat gtctggttct gttttggatg tattagtgaa aaaaggcgat
    62521 aacgttaaaa aaggtgatac gttgatgatt actgaagcga tgaaaatgga aacggccatc
    62581 gaagcacgtt ttgacggaga agtcgcacat gtatatgtgt cttcaggaga cactatttcg
    62641 tctggtgacc ttttgattga agtgactgag aaataataag cgaaaggtgg ggaaacatga
    62701 aaagactggg tttgttttgg ctgattctgc tattagttat tgtggcattc tacatggaac
    62761 cagtcttgtt tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa atatcaaacc
    62821 aaatgacaac gtggaaatac caggagctta aagcaactgg tttttctact tatatcggaa
    62881 aaccgatcga tcagctggaa aaggattttg gccaagccta tgatcgatta actagcggat
    62941 ttggttttga aacaagatat tatgaaaata aagctagtca tctcacattt gaagcgaaca
    63001 tccaagacag cagagtcaca actgttaaag tactgcaaac agggcctgct gatatcgcgc
    63061 cgttttattt agggatgacg atgcaagatc ttacgaaaat cactactatc tatactaatt
    63121 tcacttttga gtaccatgat acagaagttg gaatcgaact aatggaagag gatatgaatt
    63181 atcgtccgct aattgctttt gataacgaca cttttgctat tttatttttt gatcaaacaa
    63241 caaacggcct attttcagtt gtgtatttag ataaagatag tcttttgaaa ctattgccat
    63301 atcaagtgtt tggtgaaggc cttccacatt atcaagcaga taaatcggct gattgggaat
    63361 caatcaatcg cgttaaagaa cgaaaaagta cgactttatt aaatatgctt agaagagaag
    63421 acgaacttcc catatacaat caaacacttg cctttcaaaa ccacagtcag ctgctactac
    63481 atgatttttt gacaaggcct gaggaaatat tgtcagagga acgtatggct gattggcaaa
    63541 aatcgctagg cagtgctcaa acgacagtga aattctcact gacgaatgat gaattggaga
    63601 agttaatcag taaacaaaaa ttagaaaata ctagtgggct atttattcat ccggcgattg
    63661 acgctacttt ttcgttttta tattggtaca gcgatcctta caaccatgac cgtttcatgg
    63721 acccgggaac ggattctgta ggcattgctt tttctaaaga aaatatgtta gtcttattgc
    63781 aagaagaaaa cggagaaagt agtgatagca gtgatcgtta acgaagagct gtttcaactg
    63841 gaggatcaat gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat ggcttcttat
    63901 cttgaacata aacagtcaat ggaagaatct gcaaaagttt gccagttgaa agaagcgttc
    63961 ttgtctaaga aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc acctgattat
    64021 cgtgaaaaaa aacgagctgt tcgaaaagca aaaagacagc ttgatatgga tgagcatgtt
    64081 gcagctttcc gatatagtga aacacagctg caaagtctgt tggatcaaat tgtccaagta
    64141 cttgccgaag aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt ttttgaaacg
    64201 aaaaaacact ttggatgcgg aggtaattgt catgcaatta gacaatagtg aatttgaaat
    64261 aacaaaacga cgcgggctga tcgtatgggt ctacagctta aaacaattaa agaatctgaa
    64321 aaagttcggc ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc tttacttaaa
    64381 tgaagagaca ttggaagaaa cagaagaacg aatccgtaaa ttacattttg ttcgaaacgt
    64441 agaacgttct taccgaccgg atatcgaaat gaattttgcc gaaaaaatag gaaacaaaac
    64501 agtattaaaa gacgaaggtt tcgaaattga agagctaaat acagaaatcc gattagctga
    64561 acatctttaa aaatctctta tgagcagttt ttaaataaaa aataacttgg ataacgatcg
    64621 atttttgatc attgtccaag tatttttttg gtatactagt accagtacca tttctaagga
    64681 gtgagtagat gcggataata tcaggaaatt atggcgggcg ccgtttaaaa agtttagcag
    64741 gggcgaacac cagaccaact acagataaag taaaagaatc catattcaat atgattggac
    64801 catattttga tggagagaca gtattggatc tgtttgcagg aagcggtggt ttagctatcg
    64861 aagcagtatc tagaggctgc agtcatgcgg tatgcgtaga caaaaattac caagcgctaa
    64921 aaatcatcaa agaaaacatc gaaattacga aagaaccaga gaaatttacc acaataaaac
    64981 ttgatgcgga caaagcaatc gaacggctag cagcggagaa acaacaattc gactacttgt
    65041 ttcttgatcc gccatatgca aaacaaaaaa taatcgatca aatccataaa atggaagaac
    65101 tgggactttt ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca gtcgaattgc
    65161 cagaaactgt ggctggattc catcaaataa gaaaacagat ttatggcata acggccgtaa
    65221 ctatctatag aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa gttttgatcc
    65281 ttttacaaaa ggtcacttag atacggtgga acgtgcggct aaattattcg atgaggtagt
    65341 aattggtgtt tttatcaata cttcaaagaa aagtttgttt cccccagaag aaagaatgac
    65401 attgatcaca aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc aagaaaatca
    65461 attgacggta gaaacagcaa aagagattgg tgcagatgcg ttgatcagag gcatacgaag
    65521 tatcaaagac tttgaatatg aacgagagat tgctcagatg aatcatcatc tatatgatga
    65581 attggaaact gtcttcttat tagcaaaacc agaatatagt cacgtgagtt ccagtatttt
    65641 aaaagaagta ctgcactttg gcggagatgt gagcagctac ttaccgccag tgatcaacga
    65701 agctttagct ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa acagtactga
    65761 ttatcatatt tggaatgttg atcattggat ttgtagtagt tcccttgccg tattatgtag
    65821 aatcaccggg agccacgatc gatctgaaag aactaattac ggtcaatgat aaggaagata
    65881 aaggttctgg gtctttctct cttacctctg ttggtatcag gcaagctaca ggtttcttag
    65941 ctttaaaagc taaaatgacc ccttttgaag aattgatcag tgaagaagaa ttgactggtg
    66001 gggcgacaaa tgaagaatat aatcaaatgc aacagtatta tatggattct tcccaaaatg
    66061 cagcaatcga acaagcgtta aaacttgcag acaagcctta taaaatggaa ttcaaaggcg
    66121 tatatgtcat gagtgtagag aaaacatcga atttctttgg caagctttca gttggagata
    66181 cagtaacaaa agtagacggg aaaagctttc aaagtacaga agcatttatg gattatgtaa
    66241 aaagccaaaa agttggacaa acaatcgaaa tcagttattt gcataatggt gaagaaaaaa
    66301 cagcttctgg tgatctgatc gagttaccaa cagataaaaa ggcaggaata ggtatcacgc
    66361 ttactgacca taccgagatc gaatcagata cagatgttcg tattaattct ggctctatcg
    66421 gtgggccatc agcgggattg atgttcactt tagaaatcta tgaacaagtc actggtaaaa
    66481 acttgcgaca tggtaagcaa attgcaggaa ctggaacgat caacagtgaa ggagaagttg
    66541 gccgaatcgg aggaatcgat aaaaaagtgg caagtgcgga taaagctggc gtagagattt
    66601 tctttgcacc agatgatacg atttcagacg atgttaagaa agaatatcct gaaataaaaa
    66661 ccaactacga agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg aaaatcgtac
    66721 cagtaaaaac agtacaagaa gcgcttgact atctagagaa aatgaaatga aactaaataa
    66781 ttaaagaggg gttgtgacac gagttctgtc acaacccctc tttttcgtca aaaatcttca
    66841 cgataaaaat aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg gacgattttg
    66901 ccagagctca ctgtttcttc aattttaaat gaaaggagta atgaatgatt tttactggtc
    66961 attctttgaa aaaagaacaa tgatagaaaa aatcagcaag aaaatactag cttcacccaa
    67021 attgatcatt tggatgtcta tgatagttct tatcttttgt atcgcaagtg gttttttatt
    67081 atcaaatttg ctattttata gagaaacaga agagactgct gcaaaagaaa tactttcttc
    67141 gtttagttcc agtggagaag atccatcagc tgaaggaaac gatgtagaag aggatgctcg
    67201 tccaaaggtc atgtacaccg atatcaaagg aagtgtgaag gagcctggta tctattcctt
    67261 ttcttcagaa gaacgggtct atgatgtact aaagcgtgca ggtggattat tagaagaagc
    67321 ggatagcgat cgaatcaact tttctgcgaa gatagaagat cagcaagtcc tatatatccc
    67381 tgctgtaggt gaagagctgc cagaacatct tgatcagttg gcatcctcgg aaggaaaaca
    67441 gcctacagca gattccgagc ctcccaaaat aaatatcaat acagccactc catcagaact
    67501 tcagcagatt cctgggatcg gatcagtcaa agcgcaagaa atcattcgat ttagagaaga
    67561 aaacggatcc ttccagaaag tggaagacct gcaggagatt tctggtatcg gtgaaaagac
    67621 agttgaaaaa ctaaaaaact tcgttacaat taaatagaat gattcaaaat cgactggcac
    67681 gagcaataaa tggacgctaa tggatactaa gaataatcca attctaagag cttggcagga
    67741 cgaggaggta gtatttttgg ataggaaata gcttgattgt tcgcaaagaa acattaaaaa
    67801 aaccaatgct gaatgtctat ctccaaaata aaatatctgg gattcatatt atgaatacgg
    67861 ctgtcagcgg caataacagt caagctctac gagaacgctt tgccaaagat gtacttagct
    67921 atacagcaga taaagtattt attctcattg ggacgaatga tctagcagag cataaacaac
    67981 tttctaaaga aacgtaccaa aaaatctgca gtggatgata gttcagctga aaaaagacta
    68041 tagttctgat catgctttct ttatcacgcc accagcagtc gatgagacaa aacaaaaata
    68101 tcggaataac aagttgatca aagaatatat ctctgttctc acagaagtag caaaacgcca
    68161 tgattgtgaa gtatcgattt ttatacggaa tttcttgaac aaatggattc gaagaatttt
    68221 tataatgggc taatggatga cggacttcat ttcggcagtg aagggtagga tcttttgtca
    68281 gacaggatga ttcgttatat ttaaagaata ttttgtttaa gcaatgagta ctttttaatc
    68341 aacttgaaaa aacggaggaa tataaaacat gttagaaaaa tcttcaaaag catgcgatac
    68401 gaataaagaa aaagttactg ataagtcaca taatgaacgc attccttggg accagtactt
    68461 tatggctcag gcagtactgt tatctttacg tagtacgtgt acacgtttag aagtaggagc
    68521 gacactcgtt cgagaaaaaa ggatcattgc tggcggctat aatggagcag tcagtggaga
    68581 tgtccactgc atagatgaag ggtgttacgt cgtggatgga cactgcttgc gaacgatcca
    68641 tgcagagatg aatgcactcc tacagtgtgc aaaacttggt atcccgacgg aaggttcaga
    68701 aatctatgtc acacattttc catgtttggc ctgtacaaag gctttgttgc aagctggtgt
    68761 gaaagggatc aattatctcc atgactatcg aaatgatcct tatgcccaag cgttgatcga
    68821 acaattaggt gtatctgtac accaagttgc tttagactca cagcattttt caaaacttca
    68881 aaatgaatta acaggaaaac atgtctagcg ttttagaaag gcatgttcga aatcggttga
    68941 tttttccggc aattacgtcg atgattatct gtttgttgta ttacactgac aattggggtt
    69001 atcgtttgct tcttgccgga atttttttat ggttttcgct aaaagaagga cgttttatcg
    69061 cgtacttgtg tattttgtgt agcataacgg tatccatttc ttataggcta caagagtatt
    69121 catttttaga ggaaaaaaca acatctgcgc aaattactgt atttacagat acgattcgtg
    69181 caaatggtaa ttttttgaca atggaaggaa ggttatcaga gaataaacaa aagctccagc
    69241 tttcttatca agtaaacaca gaagcagaat tgaaaacatg gcttaacttc cgaggaaagg
    69301 gaatagaatt gactgctgaa ggcgagttca tctctccagc aaaaaagcgg aatctttata
    69361 attttgacca agaagcttac ttcaggaacc atagaatttc tggacggttc caaattcaaa
    69421 agattgaaca gatcaccatg caaaatcact ggcgaaactg gcttcagaga aaaagaggat
    69481 cttttatctc ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat attaattctt
    69541 tattatgggg ctataaagat gcaggattca gagaaccaga agaaattttt agggagagtg
    69601 gtttgctgca tctgttcagt ctatcgggtc tacacatcca attttattta gggtggctgt
    69661 attatttatt tcgacgtatc ggatggccac tacacgtcag tattattccg ttgagtctac
    69721 taactatctg ttatgtttct attgcaggaa gttctatttc tgttttgcgt gctagtttgc
    69781 tgtttttatt gcgattgcta gtcaaattgc tgaataagca ttattccacc atggacttat
    69841 ttgcagttgt cttttggctt cttttactat ttgatcctcg atttcttttt catgcgggtg
    69901 gacagcttag ttttttcatg agttttttgt ttatcctgat ttcttttgat ggagattggt
    69961 tgaaaaaaac ggcctctcac gtcctgttta ctatgatcac gatgccgttg attgtatggc
    70021 atttttatga gtggcctatt ttaggtagtc tttttacatt actgattgca ccggtattca
    70081 agtttctttt tcttcctggt gtgttatttc tcacactctt caatcgctgg ctgccggaaa
    70141 ctttactttt gctgatagaa gagggattaa tcttatttga gcgaataatc gatttttctt
    70201 cagggtcgtc tctcattata ggacgtttgc cccttgtgct atctgggatg tatattttcg
    70261 gattactgat ttgtatggat aggttgaaag aacaacctgt gaaacacttg ttttatgttg
    70321 gtcttttttc ttttattttt ttgactttgc catttttacg cttttcttca acgatcgctt
    70381 ttatcgatgt cggtcaagga gacagtatct ttttgcaaag tccttttcga aaagaaacga
    70441 ttttgatcga tactggaggt cagttacact ttgaaagaga aaagtgggcg caaggtatga
    70501 tacgtcctcc tgctgaaaga aatatcgttc catatctaaa aggtcagggg ataagtgtaa
    70561 tcgataaact catcttgaca cacgacgata cggatcatgt gggagaatta cccacgttat
    70621 ctcagcattt tactatcaaa aaaatttata tcggttgggg cgctggacga aatgagccat
    70681 tacaaaggca tttatctgaa gctcaaaaaa atggtactga gatcattgaa atcaaacatg
    70741 gggacaggat cagcggttat tatgatcttt atgtattgac tccttttgaa aagggagagg
    70801 gaagaaatga agattcgatt ggtctatgga tggaatataa caataggcgc tttttatttt
    70861 taggagattt gaatcaagca atggaaaaac aattgctttt gatttatcct aatctgaaag
    70921 ccgatgtagt caaattaggt catcacggta gccgtacgtc aagtaacaca gattttcttt
    70981 cccatattga agcaaaacat gggattatat cctgcggcgt aaataatcgt tatggtcacc
    71041 cgcacagcga agtattggag acactagaag aaaatcagat aaaaacatgg cgaacggatc
    71101 aacaaggaat gatcacctat cgctggcatc cagtttttca tcctcatggg ctagtggaaa
    71161 caatgataga ctagtaattc tcatctgctc atggtaggat aatttttgaa agtggtgagt
    71221 aatttgagta tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc atcttgttac
    71281 ttgattttag gaacagaaaa gtttctccaa gatcaagttc ggacagaaat tgtcaaaaaa
    71341 atccaattag agggcgaaga tgacttaaat tttttaagtt ttgacatgga aaatgcaagt
    71401 ttagatgagg tagtagctga agcagagacg ttgccgtttt tcggagatca acggttagta
    71461 ttcgtagaga atccttattt tttaacaggt gaaaaagtaa acaatggaat cgaacaaaat
    71521 actgatttat tgacggctta tttgaaagaa ccattagaat cgactgtatt agtattcttt
    71581 gcgccatatg aaaagttgga tgaacggaaa aaagtaacga aacaattgaa aaaaacagca
    71641 attatcatca atgtccaaca gttgaatgaa aaagaagtac gtcaatatct gatgaatacc
    71701 ttagagaatt cctcaatcaa aatggaacga agtgcaattg atttattctt gaggttgacc
    71761 gacttagatt tatccaaaat gatgagagag ttacagaaat tggtccttta tggacaaaat
    71821 caacagcaaa tcacggtaag agaagtagaa gaattagttc ctaaaacgtt agaacataat
    71881 atttttgaca tgacccaata tattttaaaa gggaagacgg aacaagcttt gcgtttatac
    71941 gaagatttag ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt gctttctcaa
    72001 ttacgtttat ttttacagac aaaatttctt gtaaagattg gttatcagca ggcaaatatt
    72061 gctgaaacat tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga agtaaaaaaa
    72121 ttcgatgaac atttgctggt acagttgttt gatcaattag ttgagatgga ttatcaaatc
    72181 aaaacgggac aaatcgaaaa agaattaagt tttcaactat tcgttttgcg cacagggcag
    72241 ttgttaagaa atcgataaga agaaagacga gggcaaagca tgcgggaagg tcgtttgttt
    72301 ttgtcctttt tttgacaaat aaaaaaatcg gccgaagccg attcttttat ttagcgattt
    72361 ttttgcttaa acgagatttg tcgcggttag ctttgttttt gtggattaga ccttttgatt
    72421 cagccatatc aatagcttta acagcttctt tatataacgc atctacgttg tcagcacctg
    72481 aagctacagc atcttcaaat tttttgattg cagtacgcat agcgcttgtt tgagatgaat
    72541 ttttcacatt agcgttttcg cttgtacgaa cacgtttgat tgcagattca atgtttggca
    72601 ttagtttcac ctccgataac ttaagttctt acgcgtgtta ccgcataatt caacattcat
    72661 tattatacct aaaggcagcg tgctttgcaa tatgaacatg acaagttttt ttccttaaca
    72721 gtcttttatt aggatttttt ttcagtaaaa acggatagta caagctgaaa aaaatcaaaa
    72781 agacgctatt gtgggaggca atagcgtcaa accaaactag atttttattt atctatttaa
    72841 acaaagtttg aatgggaatg tttggtacta agttattata taacaaagag aaccgtttgc
    72901 aaagtttttt ttgaaaaggg atacgattcc ctgttgttct ttgtttttct attaagaaac
    72961 tgcagttatt ttataaccag tatcctttga tcagtaaagg ctattctggt gtaccgatta
    73021 ttttaatgta ttcatcgata ttttttatca acattttttt tatgaccaac tggttccgac
    73081 ggttccattt gccacttact tccagcacat atttatttag gggcaaacgt aagatatatt
    73141 ttgccaaata gcgatcttta agcaggccat tgatcacacg gtcctcaagc tgcaagttga
    73201 tcccagtcag catcattggc tgttcttgta aaacgtacgt atcaattaaa agaccttcaa
    73261 aagtttccac aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc actaattctt
    73321 ccattttgat attcgttgaa gtggatgcga tcattctttc ttttgtccac tgtaatctag
    73381 tataacaaaa aagagcagaa gagccaaaaa gaaaaaagag catcgaattc tataggaagt
    73441 ctcattggca cggcctaaat tattacaaaa cattgattaa aaaaagcccc tgcatcgcag
    73501 gagcatcccc tatatcgttt taataggtca atcaaaaagc agtatcaaac acattttgta
    73561 tcaaatcagg aaataataga taagctaagc ccagaatcaa aagtaccgct agaagaaacc
    73621 agccgattgt tcctatgaaa tactttaatt tttcgaacat tgtatcacct cgttgtcagt
    73681 atatcacgga tcagtaaatt gggggtgtta agaatctcct aaaatagaca aaatttgttc
    73741 tattttccta aagcaaatac ttgtaagata cagtaagtga taccagaagc aatgaccggt
    73801 ccggcggcga ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac aagtgcaaca
    73861 gtgatttgtg gatcagaagc aatcaatcct acacccttgg aagataagac agccactagc
    73921 ccaccgcaaa aaacagctac ccagccaata ggggatttaa aggcttctaa tagatcacgt
    73981 agaccgattt ctcctgttgc gatagggatt agaatagccg ctgtgatgat cgtcacccca
    74041 atattgattc ctttgccttg aagccaaggg aaaaaggaac gggaggctgg tatgagcttc
    74101 ataatcaaaa caaaagctgc cgctatcatc agactttgat ttttagctaa aattgctaca
    74161 agtaaaataa ttcctaaaaa aagccaactt tccatgaaat atattcctca ctttcttcga
    74221 aacaatcata acatgttttt agaaagttac aaaagtagga attcatcctt caagacctat
    74281 tcataagctg tttttttcat aaaataaaca aaatcataaa aaaacacttt gcttatttga
    74341 cagaagacag ccgcgctttg tatagtggac aagacttaca tttttttgaa gaatcagaaa
    74401 gatcaggtaa aggtcttagt aaacgcaagc cattcagaat aaccaatatt gtgcttcctt
    74461 cgtgtcctac aactccaaga ggtagattta aaaattgcag gaagttagaa gcaatcaaca
    74521 agagaataac aagaatcgaa aaaataatat tttgtttgat gatccgccgc atttttaaag
    74581 atagccgatg actgtagaca aatttattta agtcgctttt cattaagacg atgtctgcga
    74641 tatccatagc ggcatctgtc ccttgcccca tagcgacacc taaagaagcg gctgctaaag
    74701 caggcgcatc attgactcca tcaccaacca tcgcatttac ttggtaaagt tcattctctt
    74761 ttttgactag ttgtgttttt tcttccggag tacagcttgc atagtaatgt tcaatcccta
    74821 cttgttcagc aatggccttt gctgtgccag aatggtcgcc ggttatcata cttgtatgga
    74881 tgttttggga tttaaagtag gaaattactt gttgggtatt tgcttttgga atatctagca
    74941 gacctaaaac agcaattaac tgctgatctt tagaaagata aatcacggtt ttgccttgat
    75001 tttccaaacg ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc tcaggatcat
    75061 atgcgtgctt gccaactttc cattgcgcac ctaaataggt agtttgtaat ccaaaaccag
    75121 taatttcttc tattggtaag tattggattt cttcaggtat tgtgcaatcg aaatattgca
    75181 aaatcgcatg agcaagtgga tgcgtcgttt tttgttccat agcaacaaga agttgttgtg
    75241 ccaaatcctt ttcatcaaag aaaaagctgt cagttacaac tggttttcct ttagtgagcg
    75301 ttcctgtttt atcaaaggca atcgctttta attcagaaag cttttctaaa tggataccgc
    75361 ctttgattaa aatcccattt tttgcagaat ttgaaagagc agctagagaa gctggtgtag
    75421 cagatgcgac aagcgcacaa ggagaagcca caactaataa taccattcct cgataaaagc
    75481 tttcatccca actccaacct aggaaaaagt gggggagaag gatcattaga ggaacagcaa
    75541 gcaaaatcaa ttttacataa ttattttcga ttcgttcgat aaatgaagct gtttgagttg
    75601 gcgtattctg cgcttcttct actaattgga taatcttagc aaagacagtt tcggaactcg
    75661 tttttgttac tcgaatcgtg atgggatttc cttgattcag cgttcctgca tacatctcat
    75721 cgcctaaata tttttctaca ggcacagatt caccgcttaa tgcagactca tcgatcgttg
    75781 cctgtgtgct ttctaaaaag ccatctatcg gaacagctgc tcctttagcg acaaatacca
    75841 agttatccac ctttagttga gaaacatcta cttcttgcat ttgtccattt tcttttaata
    75901 attgggcttt ttgtggttga agattcatca aagcagtaat ttctttttga cttttattag
    75961 ttgtgtattc ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt gctccttcaa
    76021 agtaattacc gatcaaacag gcaccaatag cagctaaagc catcaataaa tccacattca
    76081 gtgtccgatc attccacaat tctttcaagc cttccgatgt ttgtttatat ccaccgatga
    76141 taatggcact gatataaaaa agtggggtga tggtaagaga gaatcggtcg aagataaaac
    76201 cgaagatcat aaataaaagg cagccgattg ttgctgcaaa agcttttttc tgattagtca
    76261 tcgttattag acctccttgt caaaacaagg atagcataat aaaaatgtaa ttgataataa
    76321 taacagctaa atgagaacga taatggttat catttggtga ttaagcttga tgttgttttt
    76381 attatttata atgattatta ttaaagaaaa taaaaaacca gaacatttct caaataagag
//
LOCUS       DARBER010000005        60190 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARBER010000005 DARBER010000000
VERSION     DARBER010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.005"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="SCK06_000341"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="SCK06_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG1150372.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="SCK06_000342"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="SCK06_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG1150373.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="SCK06_000343"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="SCK06_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG1150374.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="SCK06_000344"
     CDS             1640..2272
                     /locus_tag="SCK06_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1150375.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="SCK06_000345"
     CDS             complement(2274..3179)
                     /locus_tag="SCK06_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1150376.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="SCK06_000346"
     CDS             complement(3264..3923)
                     /locus_tag="SCK06_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1150377.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="SCK06_000347"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="SCK06_000347"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG1150378.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="SCK06_000348"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="SCK06_000348"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG1150379.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="SCK06_000349"
     CDS             6166..7251
                     /locus_tag="SCK06_000349"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG1150380.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /locus_tag="SCK06_000350"
     CDS             7248..8312
                     /locus_tag="SCK06_000350"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG1150381.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="SCK06_000351"
     CDS             complement(8561..9364)
                     /locus_tag="SCK06_000351"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG1150382.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="SCK06_000352"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="SCK06_000352"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG1150383.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="SCK06_000353"
     CDS             complement(10242..11249)
                     /locus_tag="SCK06_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1150384.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="SCK06_000354"
     CDS             complement(11256..11900)
                     /locus_tag="SCK06_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1150385.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="SCK06_000355"
     CDS             complement(12000..12758)
                     /locus_tag="SCK06_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG1150386.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="SCK06_000356"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="SCK06_000356"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG1150387.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="SCK06_000357"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="SCK06_000357"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG1150388.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="SCK06_000358"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="SCK06_000358"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG1150389.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="SCK06_000359"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="SCK06_000359"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG1150390.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="SCK06_000360"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="SCK06_000360"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG1150391.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="SCK06_000361"
     CDS             18156..19025
                     /locus_tag="SCK06_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG1150392.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="SCK06_000362"
     CDS             complement(19151..20050)
                     /locus_tag="SCK06_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150393.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="SCK06_000363"
     CDS             complement(20215..21039)
                     /locus_tag="SCK06_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1150394.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="SCK06_000364"
     CDS             complement(21050..22345)
                     /locus_tag="SCK06_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG1150395.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="SCK06_000365"
     CDS             complement(22361..22840)
                     /locus_tag="SCK06_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG1150396.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="SCK06_000366"
     CDS             complement(22846..23874)
                     /locus_tag="SCK06_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG1150397.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="SCK06_000367"
     CDS             complement(23891..24997)
                     /locus_tag="SCK06_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1150398.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="SCK06_000368"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="SCK06_000368"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG1150399.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="SCK06_000369"
     CDS             complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="SCK06_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG1150400.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="SCK06_000370"
     CDS             complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="SCK06_000370"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG1150401.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29968..30666
                     /locus_tag="SCK06_000371"
     CDS             29968..30666
                     /locus_tag="SCK06_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1150402.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30834..33473)
                     /locus_tag="SCK06_000372"
     CDS             complement(30834..33473)
                     /locus_tag="SCK06_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1150403.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34092..35123)
                     /gene="queA"
                     /locus_tag="SCK06_000373"
     CDS             complement(34092..35123)
                     /gene="queA"
                     /locus_tag="SCK06_000373"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG1150404.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35316..36491
                     /locus_tag="SCK06_000374"
     CDS             35316..36491
                     /locus_tag="SCK06_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1150405.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36495..37136
                     /locus_tag="SCK06_000375"
     CDS             36495..37136
                     /locus_tag="SCK06_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150406.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37133..38050
                     /locus_tag="SCK06_000376"
     CDS             37133..38050
                     /locus_tag="SCK06_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1150407.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38047..38718
                     /locus_tag="SCK06_000377"
     CDS             38047..38718
                     /locus_tag="SCK06_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150408.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39259..39747)
                     /locus_tag="SCK06_000378"
     CDS             complement(39259..39747)
                     /locus_tag="SCK06_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG1150409.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39948..40214)
                     /locus_tag="SCK06_000379"
     CDS             complement(39948..40214)
                     /locus_tag="SCK06_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1150410.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40214..40477)
                     /locus_tag="SCK06_000380"
     CDS             complement(40214..40477)
                     /locus_tag="SCK06_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1150411.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40953..41138
                     /locus_tag="SCK06_000381"
     CDS             40953..41138
                     /locus_tag="SCK06_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1150412.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41280..43103)
                     /locus_tag="SCK06_000382"
     CDS             complement(41280..43103)
                     /locus_tag="SCK06_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG1150413.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43701..46406)
                     /locus_tag="SCK06_000383"
     CDS             complement(43701..46406)
                     /locus_tag="SCK06_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG1150414.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46712..47659)
                     /locus_tag="SCK06_000384"
     CDS             complement(46712..47659)
                     /locus_tag="SCK06_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG1150415.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47849..48118
                     /gene="rpsN"
                     /locus_tag="SCK06_000385"
     CDS             47849..48118
                     /gene="rpsN"
                     /locus_tag="SCK06_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1150416.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48294..48656)
                     /locus_tag="SCK06_000386"
     CDS             complement(48294..48656)
                     /locus_tag="SCK06_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1150417.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48667..49788)
                     /locus_tag="SCK06_000387"
     CDS             complement(48667..49788)
                     /locus_tag="SCK06_000387"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG1150418.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49804..50154)
                     /locus_tag="SCK06_000388"
     CDS             complement(49804..50154)
                     /locus_tag="SCK06_000388"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG1150419.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50284..51795)
                     /locus_tag="SCK06_000389"
     CDS             complement(50284..51795)
                     /locus_tag="SCK06_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1150420.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52117..53469)
                     /locus_tag="SCK06_000390"
     CDS             complement(52117..53469)
                     /locus_tag="SCK06_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG1150421.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53622..54698)
                     /locus_tag="SCK06_000391"
     CDS             complement(53622..54698)
                     /locus_tag="SCK06_000391"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG1150422.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54894..56639
                     /locus_tag="SCK06_000392"
     CDS             54894..56639
                     /locus_tag="SCK06_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG1150423.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56919..57356)
                     /locus_tag="SCK06_000393"
     CDS             complement(56919..57356)
                     /locus_tag="SCK06_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG1150424.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57340..58713)
                     /locus_tag="SCK06_000394"
     CDS             complement(57340..58713)
                     /locus_tag="SCK06_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150425.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59035..59523)
                     /locus_tag="SCK06_000395"
     CDS             complement(59035..59523)
                     /locus_tag="SCK06_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1150426.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59545..60147)
                     /locus_tag="SCK06_000396"
     CDS             complement(59545..60147)
                     /locus_tag="SCK06_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1150427.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17103 a        13016 c        10989 g        19082 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    27961 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    28021 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    28081 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    28141 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    28201 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    28261 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    28321 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    28381 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    28441 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    28501 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    28561 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    28621 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    28681 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    28741 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    28801 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    28861 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    28921 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    28981 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    29041 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    29101 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    29161 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    29221 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    29281 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    29341 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    29401 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    29461 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    29521 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    29581 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    29641 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    29701 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    29761 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    29821 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    29881 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    29941 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    30001 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    30061 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    30121 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    30181 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    30241 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    30301 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    30361 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    30421 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    30481 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    30541 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    30601 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    30661 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    30721 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    30781 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    30841 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    30901 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    30961 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    31021 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    31081 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    31141 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    31201 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    31261 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    31321 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    31381 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    31441 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    31501 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    31561 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    31621 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    31681 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    31741 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    31801 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    31861 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    31921 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    31981 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    32041 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    32101 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    32161 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    32221 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    32281 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    32341 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    32401 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    32461 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    32521 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    32581 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    32641 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    32701 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    32761 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    32821 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    32881 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    32941 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    33001 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    33061 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    33121 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    33181 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    33241 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    33301 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    33361 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    33421 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    33481 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    33541 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    33601 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    33661 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    33721 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    33781 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    33841 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    33901 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    33961 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    34021 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
    34081 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
    34141 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
    34201 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
    34261 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
    34321 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
    34381 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
    34441 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
    34501 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
    34561 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
    34621 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
    34681 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
    34741 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
    34801 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
    34861 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
    34921 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
    34981 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
    35041 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
    35101 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
    35161 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
    35221 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
    35281 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
    35341 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
    35401 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
    35461 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
    35521 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
    35581 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
    35641 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
    35701 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
    35761 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
    35821 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
    35881 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
    35941 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
    36001 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    36061 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    36121 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    36181 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    36241 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    36301 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    36361 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    36421 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    36481 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    36541 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    36601 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    36661 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    36721 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    36781 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    36841 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    36901 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    36961 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    37021 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    37081 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    37141 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    37201 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    37261 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
    37321 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    37381 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    37441 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    37501 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    37561 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    37621 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    37681 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    37741 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    37801 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    37861 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    37921 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    37981 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    38041 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    38101 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    38161 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    38221 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    38281 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    38341 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    38401 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    38461 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    38521 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    38581 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    38641 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    38701 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    38761 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    38821 cttatgggta aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    38881 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    38941 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    39001 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    39061 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    39121 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    39181 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    39241 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    39301 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    39361 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    39421 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    39481 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    39541 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    39601 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    39661 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    39721 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    39781 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    39841 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    39901 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    39961 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    40021 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    40081 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    40141 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    40201 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    40261 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    40321 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    40381 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    40441 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    40501 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    40561 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    40621 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    40681 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    40741 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    40801 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    40861 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    40921 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    40981 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    41041 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    41101 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    41161 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    41221 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    41281 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    41341 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    41401 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    41461 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    41521 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    41581 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    41641 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    41701 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    41761 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    41821 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    41881 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    41941 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    42001 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    42061 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    42121 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    42181 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    42241 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    42301 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    42361 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    42421 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    42481 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    42541 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    42601 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    42661 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    42721 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    42781 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    42841 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    42901 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    42961 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    43021 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    43081 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    43141 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    43201 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    43261 ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa
    43321 aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga
    43381 agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg
    43441 tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa
    43501 attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct
    43561 tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa
    43621 aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac
    43681 tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat
    43741 ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca
    43801 aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca
    43861 gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag
    43921 aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag
    43981 atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc
    44041 atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca
    44101 tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt
    44161 gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct
    44221 ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac
    44281 agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc
    44341 gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt
    44401 ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct
    44461 gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca
    44521 gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg
    44581 atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt
    44641 tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg
    44701 atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca
    44761 ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt
    44821 gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc
    44881 ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta
    44941 ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat
    45001 tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt
    45061 tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc
    45121 gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt
    45181 tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca
    45241 tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc
    45301 gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt
    45361 atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga
    45421 ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct
    45481 tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg
    45541 atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg
    45601 atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca
    45661 cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc
    45721 ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg
    45781 tccaaatttt catctgttga taaaatcata tctttaactt tattgatact tactaggttc
    45841 gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg
    45901 ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt
    45961 gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca
    46021 ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac
    46081 tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat
    46141 tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga
    46201 aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat
    46261 ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg
    46321 atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat
    46381 cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta
    46441 ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg
    46501 acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta
    46561 cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct
    46621 attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt
    46681 cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc
    46741 cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag
    46801 acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag
    46861 caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct
    46921 ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt
    46981 tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt
    47041 ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag
    47101 ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc
    47161 ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca
    47221 ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc
    47281 ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt
    47341 ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc
    47401 gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat
    47461 cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca
    47521 aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac
    47581 cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt
    47641 taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct
    47701 ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt
    47761 cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct
    47821 aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa
    47881 aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa
    47941 agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg
    48001 tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa
    48061 attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc
    48121 caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa
    48181 agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta
    48241 cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta
    48301 gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc
    48361 ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg
    48421 atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca
    48481 atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc
    48541 gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc
    48601 tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga
    48661 tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata
    48721 gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt
    48781 gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg
    48841 atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc
    48901 ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc
    48961 ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac
    49021 ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc
    49081 gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt
    49141 ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg
    49201 aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc
    49261 ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt
    49321 gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc
    49381 agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc
    49441 agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc
    49501 tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc
    49561 ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc
    49621 aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc
    49681 aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc
    49741 ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc
    49801 tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac
    49861 ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa
    49921 aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc
    49981 ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg
    50041 gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat
    50101 cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt
    50161 ttctcttttc aataaattta gaacactctc attctaccaa attataacca ttttgccaca
    50221 aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta
    50281 tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc
    50341 gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata
    50401 attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta
    50461 tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc
    50521 tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc
    50581 taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc
    50641 gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg
    50701 taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt
    50761 tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg
    50821 gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga
    50881 aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt
    50941 aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc
    51001 acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc
    51061 gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt
    51121 ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc
    51181 tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg
    51241 cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt
    51301 ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt
    51361 ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt
    51421 acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc
    51481 gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg
    51541 tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc
    51601 tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg
    51661 tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg
    51721 cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag
    51781 ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg
    51841 ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg
    51901 tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca
    51961 ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca
    52021 agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct
    52081 aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc
    52141 taccacttca aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc
    52201 aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat agaacacagg
    52261 tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag
    52321 gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa
    52381 agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg
    52441 cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc
    52501 gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga
    52561 aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt
    52621 atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc
    52681 tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc
    52741 gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag
    52801 caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc
    52861 cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat
    52921 cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc
    52981 gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt
    53041 gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc
    53101 agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc
    53161 aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa
    53221 aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca
    53281 aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt
    53341 accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt
    53401 ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt
    53461 tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg
    53521 ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga
    53581 acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa
    53641 aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca
    53701 ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct
    53761 ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac
    53821 cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt
    53881 tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat
    53941 tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga
    54001 acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg
    54061 attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt
    54121 tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa
    54181 agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta
    54241 agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg
    54301 attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat
    54361 gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa
    54421 acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc
    54481 tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct
    54541 gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg
    54601 taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc
    54661 tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta
    54721 aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat
    54781 ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga
    54841 ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc
    54901 aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag
    54961 tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag
    55021 gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg
    55081 ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg
    55141 tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt
    55201 caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg
    55261 gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc
    55321 ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg
    55381 atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa
    55441 aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca
    55501 gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg
    55561 gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa
    55621 ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac
    55681 aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg
    55741 tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag
    55801 agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata
    55861 ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac
    55921 tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc
    55981 ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa
    56041 gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca
    56101 cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt
    56161 tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc
    56221 tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag
    56281 atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc
    56341 ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg
    56401 aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt
    56461 tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg
    56521 tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg
    56581 tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga
    56641 aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt
    56701 ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga
    56761 gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac
    56821 atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa
    56881 taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac
    56941 gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg
    57001 ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct
    57061 tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa
    57121 gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta
    57181 taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct
    57241 ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct
    57301 aattttatga catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt
    57361 tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt
    57421 gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag
    57481 cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt
    57541 catcaataaa aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat
    57601 ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc
    57661 tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa
    57721 gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa
    57781 acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa
    57841 atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa
    57901 taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg
    57961 caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa
    58021 gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa
    58081 aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt
    58141 tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc
    58201 catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa
    58261 ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc
    58321 catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag
    58381 ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac
    58441 gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt
    58501 gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc
    58561 ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa
    58621 taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa
    58681 attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata
    58741 actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat
    58801 tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg
    58861 attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc
    58921 aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa
    58981 accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc
    59041 gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag
    59101 ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg
    59161 attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg
    59221 aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa
    59281 ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt
    59341 taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata
    59401 agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa cttccggcca
    59461 tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc
    59521 catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag
    59581 catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa tctgcataaa
    59641 ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa
    59701 gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa
    59761 ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata
    59821 gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa
    59881 aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa
    59941 aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc
    60001 aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc
    60061 ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt
    60121 ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc atttattaaa
    60181 actgcctcct
//
LOCUS       DARBER010000006        55245 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARBER010000006 DARBER010000000
VERSION     DARBER010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.006"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            234..1274
                     /locus_tag="SCK06_000397"
     CDS             234..1274
                     /locus_tag="SCK06_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1150428.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1372..1560
                     /locus_tag="SCK06_000398"
     CDS             1372..1560
                     /locus_tag="SCK06_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1150429.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1618..1971)
                     /locus_tag="SCK06_000399"
     CDS             complement(1618..1971)
                     /locus_tag="SCK06_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150430.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2502..2924
                     /locus_tag="SCK06_000400"
     CDS             2502..2924
                     /locus_tag="SCK06_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1150431.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2921..3151
                     /locus_tag="SCK06_000401"
     CDS             2921..3151
                     /locus_tag="SCK06_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150432.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3649..3849
                     /locus_tag="SCK06_000402"
     CDS             3649..3849
                     /locus_tag="SCK06_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1150433.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3876..5069
                     /locus_tag="SCK06_000403"
     CDS             3876..5069
                     /locus_tag="SCK06_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1150434.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5137..6702)
                     /gene="guaA"
                     /locus_tag="SCK06_000404"
     CDS             complement(5137..6702)
                     /gene="guaA"
                     /locus_tag="SCK06_000404"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1150435.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6761..7684)
                     /locus_tag="SCK06_000405"
     CDS             complement(6761..7684)
                     /locus_tag="SCK06_000405"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1150436.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7880..8485
                     /locus_tag="SCK06_000406"
     CDS             7880..8485
                     /locus_tag="SCK06_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1150437.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8793..9809
                     /locus_tag="SCK06_000407"
     CDS             8793..9809
                     /locus_tag="SCK06_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1150438.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9950..11251
                     /locus_tag="SCK06_000408"
     CDS             9950..11251
                     /locus_tag="SCK06_000408"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1150439.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11297..11959
                     /gene="deoC"
                     /locus_tag="SCK06_000409"
     CDS             11297..11959
                     /gene="deoC"
                     /locus_tag="SCK06_000409"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG1150440.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11980..12378
                     /locus_tag="SCK06_000410"
     CDS             11980..12378
                     /locus_tag="SCK06_000410"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG1150441.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12444..13526
                     /locus_tag="SCK06_000411"
     CDS             12444..13526
                     /locus_tag="SCK06_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1150442.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13738..15300
                     /locus_tag="SCK06_000412"
     CDS             13738..15300
                     /locus_tag="SCK06_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150443.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15293..16426
                     /locus_tag="SCK06_000413"
     CDS             15293..16426
                     /locus_tag="SCK06_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150444.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16426..17382
                     /locus_tag="SCK06_000414"
     CDS             16426..17382
                     /locus_tag="SCK06_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150445.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17481..18647
                     /gene="deoB"
                     /locus_tag="SCK06_000415"
     CDS             17481..18647
                     /gene="deoB"
                     /locus_tag="SCK06_000415"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG1150446.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18671..19489
                     /locus_tag="SCK06_000416"
     CDS             18671..19489
                     /locus_tag="SCK06_000416"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1150447.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19506..20213
                     /gene="deoD"
                     /locus_tag="SCK06_000417"
     CDS             19506..20213
                     /gene="deoD"
                     /locus_tag="SCK06_000417"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1150448.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20511..21197)
                     /gene="gpmA"
                     /locus_tag="SCK06_000418"
     CDS             complement(20511..21197)
                     /gene="gpmA"
                     /locus_tag="SCK06_000418"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1150449.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(21369..21950)
                     /locus_tag="SCK06_000419"
     CDS             complement(21369..21950)
                     /locus_tag="SCK06_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1150450.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(22083..22766)
                     /gene="rpiA"
                     /locus_tag="SCK06_000420"
     CDS             complement(22083..22766)
                     /gene="rpiA"
                     /locus_tag="SCK06_000420"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG1150451.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            23085..23498
                     /gene="rpsL"
                     /locus_tag="SCK06_000421"
     CDS             23085..23498
                     /gene="rpsL"
                     /locus_tag="SCK06_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG1150452.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            23582..24052
                     /gene="rpsG"
                     /locus_tag="SCK06_000422"
     CDS             23582..24052
                     /gene="rpsG"
                     /locus_tag="SCK06_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG1150453.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            24146..26233
                     /gene="fusA"
                     /locus_tag="SCK06_000423"
     CDS             24146..26233
                     /gene="fusA"
                     /locus_tag="SCK06_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1150454.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            26405..27592
                     /gene="tuf"
                     /locus_tag="SCK06_000424"
     CDS             26405..27592
                     /gene="tuf"
                     /locus_tag="SCK06_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1150455.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27999..28307
                     /gene="rpsJ"
                     /locus_tag="SCK06_000425"
     CDS             27999..28307
                     /gene="rpsJ"
                     /locus_tag="SCK06_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG1150456.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            28338..28967
                     /gene="rplC"
                     /locus_tag="SCK06_000426"
     CDS             28338..28967
                     /gene="rplC"
                     /locus_tag="SCK06_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG1150457.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28995..29618
                     /gene="rplD"
                     /locus_tag="SCK06_000427"
     CDS             28995..29618
                     /gene="rplD"
                     /locus_tag="SCK06_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG1150458.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            29618..29908
                     /gene="rplW"
                     /locus_tag="SCK06_000428"
     CDS             29618..29908
                     /gene="rplW"
                     /locus_tag="SCK06_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG1150459.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29947..30780
                     /gene="rplB"
                     /locus_tag="SCK06_000429"
     CDS             29947..30780
                     /gene="rplB"
                     /locus_tag="SCK06_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG1150460.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30823..31101
                     /gene="rpsS"
                     /locus_tag="SCK06_000430"
     CDS             30823..31101
                     /gene="rpsS"
                     /locus_tag="SCK06_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG1150461.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            31124..31471
                     /gene="rplV"
                     /locus_tag="SCK06_000431"
     CDS             31124..31471
                     /gene="rplV"
                     /locus_tag="SCK06_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG1150462.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            31485..32141
                     /gene="rpsC"
                     /locus_tag="SCK06_000432"
     CDS             31485..32141
                     /gene="rpsC"
                     /locus_tag="SCK06_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG1150463.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            32144..32578
                     /gene="rplP"
                     /locus_tag="SCK06_000433"
     CDS             32144..32578
                     /gene="rplP"
                     /locus_tag="SCK06_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG1150464.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            32568..32756
                     /gene="rpmC"
                     /locus_tag="SCK06_000434"
     CDS             32568..32756
                     /gene="rpmC"
                     /locus_tag="SCK06_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG1150465.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32781..33047
                     /gene="rpsQ"
                     /locus_tag="SCK06_000435"
     CDS             32781..33047
                     /gene="rpsQ"
                     /locus_tag="SCK06_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG1150466.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            33105..33473
                     /gene="rplN"
                     /locus_tag="SCK06_000436"
     CDS             33105..33473
                     /gene="rplN"
                     /locus_tag="SCK06_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG1150467.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            33510..33818
                     /gene="rplX"
                     /locus_tag="SCK06_000437"
     CDS             33510..33818
                     /gene="rplX"
                     /locus_tag="SCK06_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG1150468.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33846..34385
                     /gene="rplE"
                     /locus_tag="SCK06_000438"
     CDS             33846..34385
                     /gene="rplE"
                     /locus_tag="SCK06_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG1150469.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            34404..34589
                     /locus_tag="SCK06_000439"
     CDS             34404..34589
                     /locus_tag="SCK06_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG1150470.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            34625..35023
                     /gene="rpsH"
                     /locus_tag="SCK06_000440"
     CDS             34625..35023
                     /gene="rpsH"
                     /locus_tag="SCK06_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG1150471.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            35055..35591
                     /gene="rplF"
                     /locus_tag="SCK06_000441"
     CDS             35055..35591
                     /gene="rplF"
                     /locus_tag="SCK06_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG1150472.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35752..36108
                     /gene="rplR"
                     /locus_tag="SCK06_000442"
     CDS             35752..36108
                     /gene="rplR"
                     /locus_tag="SCK06_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG1150473.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            36129..36629
                     /gene="rpsE"
                     /locus_tag="SCK06_000443"
     CDS             36129..36629
                     /gene="rpsE"
                     /locus_tag="SCK06_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG1150474.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            36644..36823
                     /gene="rpmD"
                     /locus_tag="SCK06_000444"
     CDS             36644..36823
                     /gene="rpmD"
                     /locus_tag="SCK06_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG1150475.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36867..37307
                     /gene="rplO"
                     /locus_tag="SCK06_000445"
     CDS             36867..37307
                     /gene="rplO"
                     /locus_tag="SCK06_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG1150476.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            37307..38602
                     /gene="secY"
                     /locus_tag="SCK06_000446"
     CDS             37307..38602
                     /gene="secY"
                     /locus_tag="SCK06_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG1150477.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38664..39311
                     /locus_tag="SCK06_000447"
     CDS             38664..39311
                     /locus_tag="SCK06_000447"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG1150478.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            39502..39720
                     /gene="infA"
                     /locus_tag="SCK06_000448"
     CDS             39502..39720
                     /gene="infA"
                     /locus_tag="SCK06_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG1150479.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39753..39869
                     /gene="rpmJ"
                     /locus_tag="SCK06_000449"
     CDS             39753..39869
                     /gene="rpmJ"
                     /locus_tag="SCK06_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG1150480.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39887..40252
                     /gene="rpsM"
                     /locus_tag="SCK06_000450"
     CDS             39887..40252
                     /gene="rpsM"
                     /locus_tag="SCK06_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG1150481.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            40280..40669
                     /gene="rpsK"
                     /locus_tag="SCK06_000451"
     CDS             40280..40669
                     /gene="rpsK"
                     /locus_tag="SCK06_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG1150482.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40749..41687
                     /locus_tag="SCK06_000452"
     CDS             40749..41687
                     /locus_tag="SCK06_000452"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG1150483.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41715..42095
                     /gene="rplQ"
                     /locus_tag="SCK06_000453"
     CDS             41715..42095
                     /gene="rplQ"
                     /locus_tag="SCK06_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG1150484.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            42367..43206
                     /locus_tag="SCK06_000454"
     CDS             42367..43206
                     /locus_tag="SCK06_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1150485.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            43182..44051
                     /locus_tag="SCK06_000455"
     CDS             43182..44051
                     /locus_tag="SCK06_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1150486.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            44041..44838
                     /locus_tag="SCK06_000456"
     CDS             44041..44838
                     /locus_tag="SCK06_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1150487.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            45041..45787
                     /gene="truA"
                     /locus_tag="SCK06_000457"
     CDS             45041..45787
                     /gene="truA"
                     /locus_tag="SCK06_000457"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1150488.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(46120..48789)
                     /locus_tag="SCK06_000458"
     CDS             complement(46120..48789)
                     /locus_tag="SCK06_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG1150489.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(49027..49215)
                     /locus_tag="SCK06_000459"
     CDS             complement(49027..49215)
                     /locus_tag="SCK06_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG1150490.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            49485..49847
                     /locus_tag="SCK06_000460"
     CDS             49485..49847
                     /locus_tag="SCK06_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1150491.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49899..51581
                     /locus_tag="SCK06_000461"
     CDS             49899..51581
                     /locus_tag="SCK06_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG1150492.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            51698..53734
                     /gene="recG"
                     /locus_tag="SCK06_000462"
     CDS             51698..53734
                     /gene="recG"
                     /locus_tag="SCK06_000462"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG1150493.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            53783..54784
                     /gene="plsX"
                     /locus_tag="SCK06_000463"
     CDS             53783..54784
                     /gene="plsX"
                     /locus_tag="SCK06_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG1150494.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54906..55148
                     /gene="acpP"
                     /locus_tag="SCK06_000464"
     CDS             54906..55148
                     /gene="acpP"
                     /locus_tag="SCK06_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1150495.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        17912 a        10050 c        11855 g        15428 t
ORIGIN      
        1 aaaatcacaa attttcattc aattatcatt ttcaattgca ttttataaat cattttgtat
       61 gtaatttggt atgtaagtta aaatgaaaaa agtagtccat tttgaagtac attttgtcat
      121 tttgtactcc aaaactgaac tacttacttg catatatacg cctttatagt ggagtactac
      181 atttacgaga ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca gaaatgtata
      241 cttcaataaa tggtgaatta tgtaagattg atagagctta ttctggagaa attgttattt
      301 tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga tacaaaacta ttgccacaga
      361 gaaaaaagat tgaaaatccg caccctctac tacaaacaac tgttgaaccg agtaaacctg
      421 aacagagaga aatgttgctt gatgcccttt tggaaatctc agatagtgat ccgcttctac
      481 gatattacgt ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa
      541 tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc
      601 ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag
      661 tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa
      721 gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg
      781 cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg
      841 attgtaaaat ctgttttaag tacggtttat actatagccc tgttagtact ccagcagatt
      901 ttcggatgct tactcctatt gtactggagc aagcctttag aaaagctgga acagaattgt
      961 tagagccata tcttagtttt aaagtttatg caccacagga atatctttca cgggcatata
     1021 acgatgctcc caaatattgt gcaaatatcg taaatactca actgaaaaat aatgaggtca
     1081 ttattattgg agaaattcct gctcgatgta ttcaagatta tcgcaatgat ttaacttttt
     1141 ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg atatcaggtt accactggcg
     1201 aacctgtttg ccagacccgt cgtctaaata gtcggataga taaagtaaga tatatgttca
     1261 ataaaataac ttagtgcgtt ttatgttgtt atataaatat ggtttcttat taaataagat
     1321 gaaatattct ttaatataga tttgaattaa agtggaaagg aggagattgt tattataaac
     1381 tacaagtgga tattgtgtcc tatttgtgga aataaaacaa gactacgaat acgagtggat
     1441 actatactta aaaatttccc tttatactgc cccaaatgta agaacgaaac tttaattaat
     1501 gttcaaaaaa tgaatataat aacaatcaaa gagccagacg ccaagacgca gagccgataa
     1561 tttgagaaat gaaactctca tcttatcggc tctttttgtt tatctgaatt ttactgacta
     1621 gccttcaata tttcttgctt cattgatacc ttttgctagt gcttccatta aggatagttc
     1681 tttgtctgta aagctatcca tgtatttttc tacctgtaac cttcgagtgc ttttaatcaa
     1741 attatctgta ggtaagaaaa attcatcaat agatacatgg agtaatgata caagatcata
     1801 cagcacttgt gtacttgggt gttgtccttt gttttctata tttgttaaat aacgtgggtc
     1861 aatttcaatc attgttccca cttgttcacg agtgagaccc cgtttcattc gagcctcttt
     1921 aatcgctaaa ccaacggctc taaaatcgta tttatcttct tttttacgca tactaaatca
     1981 cctctataca ttttactgtt cctatataac cataaacagg tatagaaaaa catgttatat
     2041 agtttatgag ttcctataga acactgtgga atttattaat gcgatgaatc caatttcaaa
     2101 tagaaaggtc gctgatttta tttacctata caactttata aaatttagtc atactcctag
     2161 aaagtagata aaattggcga cttaatggag ggaaaagtcg ccatgagaaa gataatctgt
     2221 catctagttc tcttaattaa gttcttatca aaaaaactta tccacctgta ctggatagat
     2281 atgaccatca atgtggtttt gcagattaca tagatagaaa aatcaaagca tatgaacaga
     2341 gaaaacaatc tgtttatgtg cttttttggt cacttaataa atatctttta ggatttctct
     2401 tcatttatgc aacaaatccc ccgttcgtgt cggggtttag tatgaaggga gataaagaag
     2461 cacatgccac tccctttcac gacagaaagg gggtgaaaag catgaaacca tcttcttttc
     2521 aagaaaccat agaaaatcag tttgactata tctgcaaaca agttattgaa gatgaacgaa
     2581 aagattactt caaacatcta gacagactat cagcaaaaga agtctcgttt tctgatatag
     2641 ataactacct tgtcaactct ttttctactg ttgatacgta cgtcactgat ttccaactat
     2701 ttaaattatt tggattaaga atcaatgttg aaagtgattt attaagtgaa gccctgcgtc
     2761 atctttcaga gaaaaaacgg aacattattt tacttcatta ctttatggat atgagtgatg
     2821 tggagattgc ggagttacta aatttgaatc gttcgactgt ttatcgtcat cgaataagtg
     2881 gactagccat gattaaagaa ttcatgaagg agtgtaaaga atgaaaacag cttatccaag
     2941 ggttcccttc cccctcattg taaaggcgac agatggcgac gtggaagcaa ttaatcagat
     3001 tgttaaacat tatcgagggt atacctcaaa acgttcatta cgtcgcatga cagacgaata
     3061 tggaaattct catatggtta ttgatgaaac cctacgtggc agaatggaaa cgaaactcat
     3121 ctctaagatt ttatcgttcg aaattaaata gtcccatttt ttcgtggaag cgtgtcgaat
     3181 cgtatccacg cttcccgatt tgggaggttt gttattccat caaagcatat tgtgtgttca
     3241 gtaggttttg ataggctaac aagccaaacg ttctttgaca accaaataaa ggcaatcgaa
     3301 tacgtttcgg taagaaaaga gctgatgaac taacacgcca tgacctacaa aattagtgag
     3361 cgattcatgt tagtaattcg aaagtaatct ttagcaggat tgctgccatg actttcttat
     3421 cgtgataatg atactcccgt atagccatag tccgagcgtt agaagcgtca caggcaatgg
     3481 gtacggctac atgagaacca tgcagaagtg aaattcctgt gagctttgct agagctgttc
     3541 gattgctaag atattcttct cttgtcagtg tattattata aaactatttg atataattat
     3601 aggtgaatat tggagttttc ctagcctata attgggaaag gagtaataat gataacggtc
     3661 acaaaaaagg atttagttga gctaggatat ggaacttctt tttcagcaga catcataaaa
     3721 aaagcaaaag aattgatgat tacaaaaggg catatctact atcaatctcg aaagctagat
     3781 agagtccctc gtgaagctgt tgaagaaatt ctaggaattc ggttttcaaa tgaaaacagg
     3841 tctgagtagt ttttgcacag taaggagtaa aaactatggt taaagaccca ataaaaaaag
     3901 caaagaatgg aacctattat tttcgtgcaa atctaggtta tgattctaaa gggaaaaaaa
     3961 ttcaaaaata tcgtagtgga ttcactacta aaaaagaagc aagagaagaa tattcgaaac
     4021 tgctattgat gaaaccagaa gaattaagtg agaataagga taaaatgacg tttaaacatt
     4081 atatctttga aattttcctt ccatggtata agacacaggt aaagctcaga acatatgaaa
     4141 atcgcttacc aactattaag aagcatttct cttattttga taaaatggct gtttccgata
     4201 ttgagcctat tgatgtgcaa aattggcaac taaaattatc aaagaagtgt aagtcttctt
     4261 atgtaagagc tgttcaagga ctattttcag ttgctatgga tcgagcaatt gtccttggga
     4321 taaccaccac aaatccatct aagattattg gcaatgtgaa aaagcaaaag tctaaaattg
     4381 aattttggac aaaagaagaa tttgaaaaag taatctctct aatttacaaa gaagattact
     4441 atcaacactt tttatttatc tctctgtggt ttttatttat gactggaatg cgaattggtg
     4501 aagctactgc cattcaatgg gaagatattg attttgactc aggagtacta accatcaaca
     4561 aaacactcta ttacaagaat caatcgaatt atagatttgt tgaaccaaaa acaaaggcga
     4621 gcgttcgtca tattgtatta gataaatgca cattaactta tttatccgaa tggaaggaag
     4681 tacaacaaaa tctaattcag acagactttg taatgagcta taacggtatt cctacccaaa
     4741 aacatacact atcatatgct attgaacgct atgctaagct ggctggtatc catcgaatca
     4801 gaatccatgg gttacggcat tctcatgctt ccttgttaat cagtatggga gaaaatccac
     4861 ttatcattaa agatcgtcta ggacacgaag atattgagac gactttagga acgtatggac
     4921 atctttatcc taacagtaat ttcgaggtag ctaacaaatt aaatggagcc atttccttta
     4981 aagaagcaaa acaaaattat gatacttcac caaagaatca atttactgta gactacttgc
     5041 gaaacaaaga aatgaaaaaa gtgcaataaa agtgcagtaa aaagataaga agagccagaa
     5101 acccttggta taagaggatt tctagctctc ctacaactat tcccactcca ctgttgctgg
     5161 tggtttagaa gtgatgtcat acacgatacg gttgacgtgg gctacttcgt ttacgatacg
     5221 tacagagatt ttttgcaaga cttcccatgg gatacgggca aagtcagcgg tcattccgtc
     5281 gattgaagtt acagcacgga taccgactgt gtagtcgtaa gtacgtccgt cacccataac
     5341 accgacagag cggatccctg gaagaacagt gaaatattgc cagatatcac ggtcaagtcc
     5401 agcagctgcg atttcttcac gcaagatagc atcagattcg cgtacgattt ctagcttgtc
     5461 ttcagtgatt tcgccaagaa cacgaatacc aagaccagga ccagggaatg gctgacgcca
     5521 aacgattgaa tcaggcatgc ctaactgagt ccctaattca cggacttcgt ctttgaataa
     5581 tgtgtttaat ggttcgatca attcaaattg catatcttct ggcagtccac ctacattatg
     5641 gtgagatttg atcgtctgtg ctgtttctgt accagattcg ataacgtcag tatacaacgt
     5701 tccttgtgct aggaaagaga cgccttcttc accagcaagt tttgttgctt cgtcatcaaa
     5761 aacatatacg aattcattcc caatgatttt acgtttttgt tctgggtcag atacaccttt
     5821 caacttgtct aagaagcgat ctcttgcatc gactttgatg atgttcaagc cgaattttcc
     5881 gcctagactt tccattactt gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa
     5941 gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga accccaacga cactagaatc
     6001 aacgccacct gaaagaccaa gcaagacttt tttgtcacca acttgttcac ggattttttc
     6061 tacttgcata tcgataaagt tatccattgt ccagttacct tcacagtgac aaacatcaaa
     6121 ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga cgtacttctg catggaattg
     6181 gataccataa aaattacgtg ttgtgtcttg gatagaagca atcggacaat ctttgcttgt
     6241 tccgactgtt tcaaagcctt caggaacttt agttaccaag tcaccatggc tcatccatac
     6301 tgtttgttgt tttggtgttt ctttaaacaa gacagcttga tcgcttgtta cttctaacaa
     6361 ggctttccca tattcgcggt ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat
     6421 caattgcata ccatagcaaa tccctaatac aggaatacct aaattgaaga tctcaggatc
     6481 gatcccaaat gcattctcat catatacact gtttggtccg ccagaaagaa tgattccctt
     6541 tggattcatc gcttttactt cttctgcagt aatgcggtgg ctcaataatt cggaaaagac
     6601 tccaagatca cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt agtctaatac
     6661 gataatcttt tcaacgttcg tcatatcggc aacgtttgtc aaatttatta cccctatccc
     6721 tttaaatttt atctcaaaat agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa
     6781 tcgaatcgat gatatgattt tcagttttat ggagaattat atcggctctt cctcgtgtag
     6841 gcaagatata ttcctgtaaa tttttcaaat tcacattttt ccatacatct tttgccattt
     6901 taaaggcttc ttcacgatct ccgatagcaa aacgataata ataattctct ggattttgga
     6961 aagccgtatc cagtaacgca ccaaaacgat ccaaatacca tttttcgatc agcacgggat
     7021 cggcatcgac atagacagaa aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag
     7081 gtaattgaag cgtattgatc ccctcaacga tcaagatatc aggctgctgg atcaattcgt
     7141 acttgtctgg aatgatatca tacacatcat gtgagtaaac aggtactttt atctcttcct
     7201 gaccagattt tacacggttc aagaaatcga tcagcatctc catatcataa ctttcaggaa
     7261 atcctttacg gtccatgatt ccacgctctt ttaatatacg atttggataa agaaatccgt
     7321 cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca
     7381 gtctggctgt tgtacttttt ccaacggcta cacttccggc gatcccgata ataaaaggcg
     7441 gtacttctac gtatttatga agaaataaac ctttactcaa gaacaaagat tcaaattctt
     7501 tcatataaag ataaatcaga tgagcaagag gtacatagat atctcgaaca tcctgcatgg
     7561 aaatgcggtc attcaaactt ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc
     7621 cattacgata aaattctcgc cattcttctc ttgccacacg gtagtagttc attggatcat
     7681 tcatattagc ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa
     7741 accccaccac ttaagagaga ttgccgatct ttttgtataa tctttataat agaaagaaac
     7801 ggagtcttat tcttgacttg cgcacggata cataaaagga tagactgttg tcatgaaaga
     7861 aatgagaagg atggaagaca tgacaaatca ttactattct gaacgcccag atacggcgca
     7921 cgattttgaa caatgggctt ttgagttaaa aggcagaacc tttcaatttg taacagattc
     7981 tggtgtattt tcacgtgaaa cagtggatta tggttcacgt gtcctgattg atgctttcga
     8041 ttgggaagag ttacctgatg aaggaaagat cttagatgtg ggatgcgggt atggaccgat
     8101 tgggttagct ttggcatttg cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg
     8161 agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca
     8221 tcaatcgaat atctatgaag cagttcatga agaaacctat gcagctatcg taagcaatcc
     8281 gccgatccgt gctggaaaaa aagttgtaca tgaaattttg acaggcgcat atcctcggtt
     8341 gaagaagggc ggtacactga caattgtgat ccagaagaaa caaggagcgc cgagtgcgca
     8401 aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc acaaaagata aaggatatta
     8461 tatcatcaaa agtgtgaaag aataactagc tgaaaaatag tggagcgacg aaagagtgga
     8521 actgggcgtg catgtacggg tacgtatcag ttcttttttt gttgagaagt ttttaaaaac
     8581 aaagaactat tttttcttca aaaagaaata gtaggaacag agtcgttgtt ttttacgata
     8641 aaataaataa gatacagaac ttgtatagaa ggctgccaag tcggtagata aaggaggttt
     8701 ttatcaaaaa acatactttc cttttttctc tattcttttc ttgggggttt tggtacactg
     8761 atagggaatt tagatagaag ggagccaata ggatgaagct gaccatcaga gatatcgcag
     8821 aaatggcagg cgtatcagta acaactgtct cacagatttt gaataacaaa ggaagcagat
     8881 ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg
     8941 attatttcgc ttcaaatgtg atcaatcgac attcaaagac catcggaatg atcgtccctg
     9001 atgtcactga tttcttcttc tccaaactga tcgaaggtgt agagagctat ttgaatcctt
     9061 tgggctatgt gatcatgtta tgcaattctc gtcatagcca agaaaatgag atcaagtatt
     9121 tacaagagct ttcccaccgc tcagtggacg gaatcttgct ggccacacca aatattttac
     9181 ctgagaaata tgcattagac agcggttttt atcaaaaaat gccaatcatt ttaatcgatc
     9241 gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc
     9301 aagctgtttc gctgtttatt gaaaatggcc atacgaagat cggcatgctg aaggaaacca
     9361 ctggatacta tcaattagaa gaacgtttca acggatatcg tcatgcgctg aaagatcatg
     9421 gcctgccttt taatgggaaa tttgttgaac atggagagtt gactgtccaa ggtggatata
     9481 ctgcatcaaa aaaattactc aaacataaag atatcacggc tattttttgc gggaatgatg
     9541 ccatggcgat cggatgctat caggcgatcg atgaattggg caaaaaaatc ccagaagata
     9601 tctcagtcat aggattcgat ggattaaagc tgtcagaata catgattccg caattgacga
     9661 cagtgcagca gccaagtttt gatattggat tttatgctgc acgtttcctg atcgatacga
     9721 tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta attattcgtg
     9781 agagcgtaaa agtattgaca aagggctaac tcggcggtat attgcttatg taagcattta
     9841 caaaggctag ttagtgcttt ttttattttt agtaaaccag tttactaaag tggtttacct
     9901 ttcctaaaaa taatgtataa taccgaatga agacaaggag gatcaggcaa tgcgaatggt
     9961 ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat
    10021 cgtcacgaac tatacgaatg gaaagattcc agattatcaa gtcagtgcat tattgatggc
    10081 aattttttat caagacatga caaatgaaga aatcacgaac ttaacattgg cgattgctaa
    10141 ttctggtgat gtcatcgatt tgagttctct tgaaggaata aaggtagata agcattcgac
    10201 aggtggcgtt ggtgacacga ccacattgat tttagccccc ctagtagcta gtgtcggagt
    10261 gacagtggct aaaatgtctg gacgcggtct aggttacaca ggtggtacac tagacaagct
    10321 agaagcaatt cccggatttc agatcgagct ttcggatgaa gaatttgtac gcattgttaa
    10381 tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt
    10441 gtatgccttg cgtgacgtca cagcgaccgt tgattctctt ccgctgattg ccagttcgat
    10501 tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga ccactggaga
    10561 cggtgctttc atgaagaaca tcgaagatgc aagacgtctg gcaaagacca tgacgagcat
    10621 tgggaaactg gctaaccgag aaacagtggc ggttatctca gatatgtcag aacctcttgg
    10681 cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc gaaacattac aaggaaatgg
    10741 tccagaagat ctagtagaaa tgtgctacgc cttaggaagc caaatggttg ttcttgctgg
    10801 caaagctaag acaaccgatg aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa
    10861 agcgttagca aaattcaaag aaatgatcca aaatcaaggg ggagacccag cgatcgtgga
    10921 acatccagaa cgaattttga ctgcacgcta cacaatggag cttcctgcta aacaaagcgg
    10981 agttgtgtca aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc taggtgctgg
    11041 gcgtaaaacc aaagaagaag atattgatca cgctgttgga ttgaaactgc ataaaaaaat
    11101 tggtgacact gtgacaaaag gtgagtcact attgacgatc tatagtaacg acgaagaaat
    11161 cacttctgtc attgaattat tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc
    11221 aacgttgatt catgacatca tcacagaata aaggaaaaaa gcggacttta ccgttttaaa
    11281 gaaaagggga aaagaaatgg aactaaaccg aatgatcgac catactattt tgaaagcaga
    11341 cgcatctaaa gaagctgtga tgcaaattat cgaagaagca aaaaaatacc atttttattc
    11401 tgtctgcatc aatccagcat gggttgcgct agcgaaagaa caattacaag gtgagccagt
    11461 agccgtatgt acagtaatcg ggtttcctct aggagcaaac acacccgaga caaaagcatt
    11521 tgaaacaaca gatgccatca ataacggtgc agatgaagtc gatatggtca tcaatatcgg
    11581 tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc
    11641 agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca tcgttattag atagagaaga
    11701 aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca aaacatcaac
    11761 tggtttctca acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg
    11821 accagaaatg ggtgtcaaag cttccggcgg tatccataac gaagaagaag caatggcaat
    11881 gatcgaagct ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg tatctggttc
    11941 aactggtgaa ggctattaat tttttcaaag gagaagctca tggcagaact caaacaagaa
    12001 tggatcaaca cagctattga agcgttagat aaagcctatg ttccttattc ccattttccg
    12061 gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa cattgagaat
    12121 gcatcatttg gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa
    12181 ggagaaaaaa catttacgca tctagtcgta gcggggcaca cccccgatcc tatctctcct
    12241 tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc ggttacgcta
    12301 gtaggagaca atggtgtgac gaaggcaacg accgttcgtg cactgctgcc ttatgcattt
    12361 actgaaaaag atctataact catgttcatt cagtcagctg actgttgaaa aataaaacaa
    12421 ttattttagg aggctttaca gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg
    12481 cactagcagc agggttattt ttaggtgcat gcggcaacaa cggctctact gattcatccg
    12541 gtggcaaaag ctcatcagac acaacaactg cagcattgat cacagacaca ggtggtgtag
    12601 acgaccgttc attcaaccag tcagcttggg aaggactaga aaaatggggg aaagatcaag
    12661 gactttcacg aggaaatgat ggcttccaat atttccaatc ttcaaatgaa tcagattaca
    12721 tcccaaatat tgaccaagca ttgaatgctg gattcaaaac gatttttggt attggttata
    12781 aactaaaacc agcgatccaa gaacaagcaa caaacaatac aggtacgaac tttgttatta
    12841 tcgatgatgt catcgatgga ttagataacg ttgtatctgc aactttcaaa gacaacgaag
    12901 cttcttatct tgcaggtgtt gcagcagctt atacgactga aacaaacgta gtcggcttta
    12961 tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa gccggtgttg
    13021 atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact
    13081 tctctgctcc agataaagga cgttcaattg ctcaaggtat gtatgcacaa aacgcagata
    13141 tcatcttcca tgcttcaggc ggtacaggta acggtgtatt ccaagaagca aaatcattga
    13201 acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag
    13261 gcgaatacac attaaacggc gaaaagaaaa acttcacatt gacatctaca ctgaaagcag
    13321 ttggaacagt agtagaagat ttggcacaaa aatcagcgga cggtaaattc cctggcggcg
    13381 aacacactgt ttatggattg aaagaagacg gtgttggtct aacagaagga caactttctg
    13441 acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag
    13501 ttccagaaac tcctgaagaa aactaaaacc atttctatag ccttgttaga cacatgtttg
    13561 acaaggctat ggtttgatta gaacaagaat tgttcattta tccaagctgc cttgtgacag
    13621 tttggataag ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa
    13681 gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg
    13741 gttcaagaaa atattgtcat agaaatgcag aatattacca aagaatttgg aacattcaag
    13801 gcaaatgaca acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa
    13861 aacggtgctg gcaaatctac attgatgaac gtgctttccg gattattaga gccgacatca
    13921 gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa agccaaccaa
    13981 ttagggattg ggatggtcca tcagcacttt atgcttgttg atgcctttac tgtaacagaa
    14041 aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc ttgaccataa aaaagcacga
    14101 aaagagatcc aaaaagtttc tgaacagtat ggattgtcag tcaacccaga tgcttacgtt
    14161 cgtgatattt cagttggaat ggaacaacgg gtagaaattt taaaaacact ttaccgagga
    14221 gcagatgtac tgatttttga tgagccgaca gctgtattga cccctcaaga aattgatgaa
    14281 ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt cgatcatttt gattacgcat
    14341 aagttagatg aaatcaaagc agtagctgac cgctgtacag ttatccgccg tggaaaagga
    14401 atcggtacag tcaacgttaa agacgtaacc tcacaacaat tagccgatat gatggttgga
    14461 agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct
    14521 attgaaaatc tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta
    14581 gaggttcgtg ctggcgaagt acttggtatc gctggaatcg atggaaacgg gcagtcggag
    14641 ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg
    14701 gaagacatca ctaataaaaa accccgaaaa atcactgaac atggtgtagg acacgtgcca
    14761 gaagaccgtc ataaatacgg gttagtccta gatatgacat tatctgaaaa tatcgcacta
    14821 caaacctatc atcaaaaacc ttacagtaaa aacggcatgc tgaattattc agtaatgaat
    14881 gaacatgcca gagaattgat cgaagaatat gatgttcgaa caacgaatga acttgttccc
    14941 gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga
    15001 gatcctgatc tgttgattgt tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa
    15061 tttattcata aacgtctgat cgaacaaagg gacaaataca aagcagtgtt actgatcagt
    15121 ttcgaattag aagaaatttt aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa
    15181 attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct attgatggcg
    15241 ggctatacct tagaagaagc acgaaaagag ttagaaaagg gggctggcgc acatgaatga
    15301 tcgcagcgaa aaattccgta atttattagt gcctgtactt tcagttgttt taggattgat
    15361 cttaggcgcg atattgatgg ctgcttttgg ttttcagccc atccaaggtt attcttctat
    15421 gctagatgca gcccttggtt cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc
    15481 gttgattttt acagcattag gtttttctgt agctaactca gctgggtttt tcaatattgg
    15541 gctttctgga caagcacttt gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc
    15601 agatctgcca aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc
    15661 gatggcagcg gctgttccag gtttacttcg agcatttttt ggaacaagtg aagtgatcgt
    15721 aacaatcatg atgaactata tcttattata cgtaagcaca tttgctttac atgaagtgat
    15781 gcctgaaagc tatcgctcaa gtttggactc ttccaatatg atcagtcaaa atgcctcact
    15841 gcggttacca tggttaacac aagcttttgg cggctctcgc gtaaactcag gattattttt
    15901 agcaatcatc gcgcttattt tcgtttggct gctgatgaag aaaacgactt taggatttga
    15961 aatccgttct gttggattga atccttttgc ttctgagtat gcaggaatga gtagtaaacg
    16021 tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca gggcttggcg gtgtggttga
    16081 aggattagga acgtatcaaa acttctttgt tcaaacgact tcgctatcta ttggatttga
    16141 cggaatggct gtttccttgc ttggttctgg atcagcaatc ggtattcttt tatcagcgat
    16201 cttgttcagt attttgaaga tcggtggact tggcatgcag acaggagcag gtgttccgtt
    16261 tgagatcgtc aatgtatcga ttgctttgat tatcttcttt gtcgggatca acttcctgat
    16321 tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat
    16381 agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa ctataatgtc gactatcgaa
    16441 ttaattgcct tgatccttac ttctacactg gtttattcaa caccgctaat tctaacgtca
    16501 ctaggcggaa ctttttctga acgaagcggg atcgtaaacg tagggctaga gggaatcatg
    16561 gtcatgggtg cttttagttc catcgttttc aacttgacga tgagtccatc acttggttct
    16621 atgacaccat ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt
    16681 cacgcagtag caacagtcag tttgcgtgct gaccatatta tcagcgggac agtaattaat
    16741 ctgatggcac ctgctttagg tgttttcttg atcaaagcat ggtatggtaa aggtcaaact
    16801 gataacatca cgcagaatct ggggtatttt tctttcccag gattagcaga tattccggtc
    16861 attggaccaa ttttctttaa aaatacattc ttacctgctt attgtgctat tttgattgct
    16921 gtcctagctt gggtgatcat cttcaagacg aaattcggct tgcgcctccg ttcagttggt
    16981 gaaaatcctc aagctgccga cacattaggt atcaatgtat atgctatgcg ttattcaggc
    17041 gtactgattt ctggcttgtt aggcggtatg ggcggtgctg tttttgctca gtcgatttcc
    17101 ggtaactttt cagctggtac gatcgttgga caagggttca tctccatggc tgctatgatc
    17161 tttggtaagt ggaatccttt aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa
    17221 agcttgagta tcattggcgc acaattacca gttatttctt cgattccgcc agtattgctg
    17281 caaattgctc catatttgct gacaatcatt gtgctggttg ttttcttagg gaaagcttct
    17341 ggtccaaaag caaacgggaa gaactatatc aaatcaaaat aatcttttag cgaataaaaa
    17401 gggtgggtta cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc aataaaaata
    17461 aaaagaaaga aggaataatc atgtttaaac gcgtacatct gatcgtcatg gattcagtag
    17521 gaattggaga agcaccagat gccgataagt tcggtgatgt tggttctgat acattaggac
    17581 acattgcgaa agaagcagga ttgaacgttc cacatttaga gcagcttggt ttaggaacga
    17641 tccgtcctct tgaaggtgtg gaatcagtga aagatcatca aggctatgcg acgaaattag
    17701 aagaagtttc tgtagggaaa gatacgatga caggccactg ggaaatcatg ggattgaata
    17761 tcaaaactcc atttcgtgtg tttcctaatg gattccctga tgatctgatc aaacaaatcg
    17821 aagaattttc tggccgtaaa gtcgtatgta acaaacctta tagcggaaca gctgttattg
    17881 acgattacgg tgaacaccaa atgaaaacag gcgatttaat tgtatatact tctgccgatc
    17941 ctgtccttca gatcgcggca catgaagaaa tcattccttt agaagaatta tatcggattt
    18001 gccaattcgt tcgtgatata acaaaagatg atccgtatat gattggccga atcattgcac
    18061 gtccgtacgt gggtgaacca ggagacttca cacgaacaag caatcgccat gattatgcgt
    18121 tgaatccatt cggacacaca gtccttgatt ctttgaaaga agcaggaaag gacgtgattg
    18181 ctgttggaaa gatcaacgat attttcaacg gacaaggaat cactgaatct gtccgcacga
    18241 aaagcaatat ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa
    18301 tcagctttac caatttagta gattttgatg cattatacgg tcatcgtcgg gatgtagtcg
    18361 gctatgcgca tgcgattgaa gaatttgact tacgaattcc agaattgctt gaagcgatgg
    18421 aagaagacga cttgctaatg attacagccg atcacggaaa cgatccgacg tttactggta
    18481 ccgatcatac gcgagaatat gtaccgttat tggtttacag taaaaaaatg aaagaacaag
    18541 gggcattgcc tcaaggatat tattcagata tttcagcaac gattgctgag aactttagtg
    18601 tttcttcgac tgaaaatggc gaaagcttct tgaaattatt aaaataatct ttttaaatag
    18661 gagaaagcaa atgaccaagt taagcgagat gttacaagaa acgacccagt ttatccttga
    18721 taaaggagta aaagaaattg attttggttt gattctaggt tcaggattag gtgaattagc
    18781 agatgaaatc gaagatgctg taatgatttc ttatgatgag atcccgttct ttcctgtttc
    18841 gacagtagtt gggcatgcag gaaagctagt ttatgggtct ctggcaggca agaaagtgtt
    18901 agccatgcaa gggagattcc actactatga aggtcattcc atgcaaacag tgacctatcc
    18961 agtacgtgtg atggccggac taggggccca ttcagtcatt gtgactaatg catgtggtgg
    19021 tgtcaacgaa tcatttgtac caggagattt gatgctgatt accgatcata tcaattttac
    19081 tggtgaaaat cctttgatcg gtccgaatga agatgcaatg ggccctagat ttccagatat
    19141 gagtcaagct tatacgcctg actatcaagc aacagcaaaa gcagcagcag aaaaactagg
    19201 tttggatctt aaagaaggcg tatatatggg ctattctgga ccaacttatg aaacgcctgc
    19261 ggagatccga atgtctcgga caataggagc cgatgcagta gggatgtcga ctgttccaga
    19321 agtcatcgta gcagctcaca gcggattaaa agtgttagga atctcttgta ttaccaactt
    19381 agcagcaggt atgcaagcga atctgagcca tgaagaagtt gtagaaacaa ctcagagagt
    19441 caaacaatct tttaaagcat tggttaaaga aacattgaca ttattgtaat aggaggaaca
    19501 ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat cgccgataag attttacttc
    19561 cgggagatcc tttgcgagct aaatatattg cggagacttt tttggaagat ccagtttgtt
    19621 acaatcaggt acgcggtatg ttaggcttta ccggcaaata taaaggagaa cgtatttctg
    19681 ttcaaggaac aggaatggga atgccatcag ccaccatcta tgcccatgaa ttgatccagt
    19741 cgtatggtgt aaagaaattg attcgagtag gtacatgcgg tgcattgtca aaagacgtcc
    19801 atgttcgtga tttagtacta gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa
    19861 atttccaagc ctttcatttt cctccaatca gtgatttcaa tctattactg aaagcatatg
    19921 agatagctaa agaaaagggc tatacggttc atgtaggaaa tgttctttcg gaagattctt
    19981 tctacaaaga tgatttgaca gagacgttcc aattagctga acttggcgtt ttaggagtgg
    20041 aaatggaagc agcggctctt tattacttag gcgcgaaata ccatgtgcag acattaagtt
    20101 tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat
    20161 ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc gatagcagaa taagaaggaa
    20221 atgaaaaagc tgccctgcat taagtaataa gaacatccaa gcaaaaatag ctcctcatgt
    20281 ttttgacttg gctggactta ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag
    20341 gttgtgaatc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa cagcttttca
    20401 tgtttgatga atattgtgac ttgtgaccga gaagttggca ggagaaaacc gtttatacga
    20461 aaaaagggac tgtgacaaat cgtgtcacag tccctttttt atagtgtttt ttataggtag
    20521 tattttttag caactgttaa gtcatcattt aattcataaa caagtggttg acctgttgga
    20581 atttcaagat ccatgatgtc ttcgtcagag ataccttcga tatgtttagc tagggcgcgt
    20641 aatgagttac catgtgcagc tactaaaaca gttttgttgt ctagtaatgc tggagcaatt
    20701 tcatcttgcc agaatggcaa agcgcgttct aatgtcactt tcaagttttc tccaccagga
    20761 atatcgcgtt tgtctaacat tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt
    20821 tcttccatta atggaggaag agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg
    20881 ccgtattttt cagctgtttc ttttttgttc aaaccttgta atttaccata gtgacgttcg
    20941 tttaagcgcc atgatttgat ttgtggtacc caaagttgat ctgaatattc taagatcaag
    21001 ttacatgttt tgattgcacg agttaaaaca gaagtgtaag caacatcaaa ttcgattcca
    21061 gcttctttga ttttgcgtcc gccttctttt gcttcttcga ttccttctgg tgctagattt
    21121 acatcagccc aaccagtaaa ttggttcaat gcattccatt cactaagacc gtgacgagaa
    21181 aaaactaatt ttggcatgtt tagctctcct tttaagatag gtttatattt ctgaaatttt
    21241 cagttctgtt ttattgtaca attttttgga agagatttcc atcgaaaaat tagataagat
    21301 tctatgagaa aacaagtaga gaatataaaa aaacgaacag tgcccacgca actgttcgtt
    21361 tttaggtatt agatgcataa ttgctcgatt tctttttcag gtaaattgat atatttataa
    21421 catgttccaa ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc ttttttttca
    21481 taatagagac gtcggatttt tttgatcgtt tttgcatcga tttttggtct tccgccgatt
    21541 tttccttttt tacgagcttc atctaatcca actaatgtac gttctttgat caatgcgcac
    21601 tccatgtttg ctaggttttc cattaattgg aaatagattt ttcccatttc gcgcgtatcg
    21661 attccttcat caagactaat caagtgcagc ccgcgatctt tgaataattg cgtgagttct
    21721 gttaattgcc gcgtagattt gcctaatcga tgaagttggc aaatgactag tgcatcgcct
    21781 tgttgcatgt cagcgatcac tgactccaaa tgaacagcag tagaatcatt ttgttccact
    21841 tcatatgtct catggaaaat ttcttgacaa ccaaagtcag ataattgatc aacctgagtt
    21901 gttaaatcaa cgtcatttat ggttgtgcgt gcataaccaa taattttcat ggtgattcct
    21961 ttctagcagt gattttctaa ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa
    22021 aagatagttt tttcgcaaat tttccgctgt ctttttttat tggatgatca agcagttttt
    22081 ctttatttac gacggattgc gtcaagaatc tcaacgcctt tttctgagcc gacaactact
    22141 ttagaaacca tgtttaggaa aagcccatgt tctactaccc caacaagttc atctaaatag
    22201 gtagctaagc tttctggctg ttcgatgact tctagatgca gatcaatgat gtaatgacca
    22261 ccatctgtgg ttaagacttc tccgttttcg tctgtacgca agacgggttg gaatcttttt
    22321 tcttcaaaaa tatgaagtag ctgttgggag ccataaggga tcacttcaac tgggagtggg
    22381 aacttcccaa gtttatcgac taatttagaa gaatcaacga tccaaatcgt ttcttttgag
    22441 tacgtagcga ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa
    22501 tcagcactga tttcatcagc accgtctatt gtcaaatcga ctacggggac ttcatcaata
    22561 ctctttaacg gtattcctaa tttttccgct tgttcttttg tcgcatttga agtagtaact
    22621 cctgtgatac gtaaaccttc ttcacgcata cggcgaccaa tctcttctac catataatag
    22681 gcggttgaac cggtcccgag acctacgatc atcccgtcct ttacgaattt cgcggcttca
    22741 atgccagcta attgttttaa attcatcttt gcgtccacct ttttttagtt tgagcatcgt
    22801 gctttcctca ctattttagc gtgatttacg gtaaaaagat accattcttt tttattccaa
    22861 taaagctttt tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt cgtgatattc
    22921 taacaaaggt gctttatcta caggtttctg tgaagaaacg tgctgactgt ttatcaaagc
    22981 atatttgata aggatgcaga cattgcattt attttttatc acaaaaaaag aaccacctgg
    23041 atgtgtggaa ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct acaattaacc
    23101 aattagtacg taaacctcgt aaatcaaagg tggaaaaatc taattcacct gctttgaaca
    23161 aaggatataa cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg
    23221 tagcaactcg tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg
    23281 cacgtgttcg tttgtcaaac ttaattgaag ttactgctta tatcccaggg attggtcata
    23341 acttacaaga acacagtgtt gtattattac gcggtggtcg tgtaaaagac ttaccagggg
    23401 tacgttacca tatcgttcgc ggtgctttag atactgctgg tgttaacgac cgtaaacaaa
    23461 gccgttctaa atacggaact aaaaaaccta aagcttaatt ttaattatta aacaaatgaa
    23521 acttgcttct actatataga agtaattact atgaacaata tttcggaagg aggagttacg
    23581 gatgccacgt aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta tttataactc
    23641 aaaattagta actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc
    23701 taacattatc tataatgcct ttgacttgat caaagaatct acaggtaacg atccattaga
    23761 agtgtttgaa caagcaatga aaaacgttca acctgttctt gaagttaaag cacgtcgtgt
    23821 tgggggttca aactaccaag taccagttga agttcgccct gaacgccgcg ttactctagg
    23881 tcttcgttgg gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt
    23941 agcaaaagaa atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga
    24001 cactcataaa atggctgatg ccaaccgtgc gtttgctcac tatcgctggt aagattctct
    24061 ctgtttggtc ggcttttata agtcgacctc agatagtgtc cttattttta gcaactagat
    24121 agtaaagaga ggagaaacaa ggagaatgac cagagaattt tctttacaaa acactcgtaa
    24181 tatcggtatc atggcccaca tcgatgctgg taaaacgact gctactgaac gtatcttgta
    24241 ctatactggt aaaatccata aaattggtga aactcatgaa ggagcttcac aaatggactg
    24301 gatggaacaa gaacaagaac gtggtattac gatcacttct gctgctacta cagcacaatg
    24361 gaagggtcac cgaatcaaca tcatcgacac accaggacac gtagacttca cagttgaagt
    24421 tgaacgttca ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt
    24481 tgaacctcaa actgaaacag tttggcgtca agcaacaact tatggtgtac ctcgtatcgt
    24541 atttgcaaac aaaatggaca agatcggtgc tgacttctta tattcagtat caactttaca
    24601 tgaccgtttg caagcaaacg ctcacccaat ccaattacca attggtgcgg aagacgactt
    24661 cacaggaatt atcgaccttg taacaatgaa ggctgaaatg tatacaaacg atcttggaac
    24721 agagatcgaa gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga
    24781 aaaattggtt gaagctgtag ctgaaactga tgaagaactt actttgaaat acttagaagg
    24841 tgaagaaatc actgaagctg agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga
    24901 attctaccca gtactatgtg gttcagcatt taagaacaaa ggggttcaat tactattgga
    24961 cgctgttctt gattacttgc catcaccgtt agatatccca gctatcaaag ggattgaccc
    25021 taaaactgat gaagaagtag aacgtcctgc agacgacagc gctcctttct cagctttagc
    25081 atttaaagtt atgactgacc cattcgtagg tcgtttaact ttcttccgtg tgtactctgg
    25141 tgttttacaa tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg
    25201 tcgtatccta cagatgcatg ccaactctcg ttcagaaatc tctgaagttt acgctggaga
    25261 tatcgctgct gctgttggtt tgaaagacac aactactggg gacacactat gtgatgaaaa
    25321 gagtctagtt attcttgaat ccatggaatt cccagaacca gttatccaag tcgctattga
    25381 acctaaatca aaagctgacc aagataaaat gggtgtggct ttacaaaaac tatctgaaga
    25441 agatcctact ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat
    25501 gggtgaactt cacttagata tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc
    25561 taacgtaggt gcgcctcaag tatcttaccg tgaaactttc cgtgctggca ctcaagccga
    25621 aggtaagttt gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt
    25681 tacaccaaat gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt
    25741 tcctcgtgaa tacattccag ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt
    25801 cttagctggc tatccattag tagatatcaa agctaaactt tacgatggtt cataccatga
    25861 tgtcgattca aatgaaactg ccttccgtgt tgctgcttct atggcattac gtgcagcagc
    25921 taagaaagct aatccagtaa tccttgaacc aatcatggct gtagaagttg ttatccctga
    25981 agattattta ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat
    26041 ggaagctcgc gctggcggcc aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt
    26101 tggttatgct acaactcttc gttctgctac tcaaggtcgt ggtacattta caatgacatt
    26161 tgaccactac gaagatgtac caaaatcagt acaagaagaa atcattaaga aaaacggcgg
    26221 aaaagctgaa taatttctta aaaccggata gtcctgcaaa gagattatcc aaagagacta
    26281 tatttttaat atagtttcgt gtaaaataat tatcgatgat atgggcatgt ggtgtacctt
    26341 gtgtacatca tatacctatc agacaaataa aaaatatatt gtatttttag gaggacattt
    26401 taaaatggca aaagaaaaat ttgaccgttc taaaccacac gttaacattg gtactatcgg
    26461 acacgttgac catggtaaaa ctacattaac agctgcaatc acaactgtac tatctaagaa
    26521 aaacggcggc caagctatgg cttacgatca aatcgatggt gctccagaag aacgcgaacg
    26581 cggaatcaca atctcaacag ctcacgttga gtatgaaaca gatacacgcc actacgctca
    26641 cgttgactgc ccaggacacg cggattacgt taaaaacatg atcactggtg ctgctcaaat
    26701 ggacggagct atcttggtag tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca
    26761 catcctattg tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga acaaagtaga
    26821 catggttgat gacgaagaat tactagaatt agttgaaatg gaagttcgtg acctattaac
    26881 agaatacgaa ttccctggtg acgatgttcc tgtagttgct ggatcagctt tgaaagctct
    26941 agaaggcgac gcttcatacg aagaaaaaat tcttgaatta atggctgcag ttgacgaata
    27001 catcccaact ccagaacgtg acaacgacaa accattcatg atgccagttg aagacgtgtt
    27061 ctcaattact ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt
    27121 tggtgacgaa gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa cagttactgg
    27181 tgttgaaatg ttccgtaaat tgttagacta cgctgaagct ggagacaaca ttggtgcttt
    27241 actacgtggt gttgcacgtg aagacatcca acgtggacaa gttttagcta aaccaggtac
    27301 aatcacacct catacaaaat tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg
    27361 acgtcatact ccattcttca ctaactaccg tccacaattc tacttccgta caactgacgt
    27421 aacaggtgtt gttgaattac cagaaggaac tgaaatggtt atgcctggcg acaacgtaac
    27481 aatggaagtt gaattaatcc acccaatcgc tatcgaaaac ggtactaaat tctcaatccg
    27541 tgaaggcgga cgtacagttg gtgccggtgt cgttacagaa atcaaagctt aattcactta
    27601 agttttaaag taaagcaaca tttattcgga cgtaatcgct cggaacgaag gtcttaggat
    27661 ctttgttccg agctttttta tttattgcag gatcaggaaa aaagacagga gtattataaa
    27721 tagaagaaac acgaaataaa agagtcagta ttttctaaat gaaactttat gaaaacaaaa
    27781 tgttttttac aagaaaaagc cttgctttgt gagtttatat atattataat gacaaaggtg
    27841 cgtttacttt agtgaattgc acagacgtaa tcagaatgat ttcgtcggct aagaaacgag
    27901 aggttgcgac acgcccggac gctttgccac gaacgagcgt gacggaaatt ttcgtggagc
    27961 tatgtctact tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta
    28021 tccgtttaaa agcgtatgaa caccgtattt tagatcaatc agcggataaa atcgtggaaa
    28081 ctgcaaaaag aactggagct agcgtgtctg gtccaattcc attaccaact gaacgcagtc
    28141 tttacacaat catccgtgcg actcataaat acaaagattc acgcgaacaa ttcgaaatgc
    28201 gtacacacaa acgtcttatc gacattgtga acccaacacc aaaaactgtt gatgctttga
    28261 tgaagctaga cttaccatct ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa
    28321 ataatggagg tgtactcatg accaaaggaa tcttagggaa aaaagtggga atgacacaaa
    28381 tcttcactga atctggtgaa ttaattccag taactgttgt agaagctact ccaaacgtag
    28441 tactacaagt gaaaacaatg gaaacggatg gctacgaagc tattcaagtt ggttaccaag
    28501 atatgcgtga agttttatca aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg
    28561 ctcctaagcg cttcattcgt gaattcaaaa atgttgagct aggagaatat gaagtaggaa
    28621 aagaaattac agtagatgtt ttccaagcag gagacattat tgatgtaaca ggtactacga
    28681 aaggtaaagg attccaaggg gttatcaaac gccacggaca aagccgcgga ccaatggctc
    28741 acggttctcg ttaccatcgt cgtcctgggt caatgggtcc agttgcacct aaccgtgtat
    28801 ttaaaaacaa acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg
    28861 aagttgtacg tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat gttcctggag
    28921 ctaaaaaatc tttaattact atcaaatcag ctgtgaaagc taaataattg taggaagaaa
    28981 ggaggaacta aggaatgccg aatgtagcat tattcaaaca agatggaagc caaaacggtg
    29041 aaatcacact taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg
    29101 caatcgtaat gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa aaccgtagcg
    29161 ctgttcgcgg cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag
    29221 gatctatccg ctcaccacaa tggcgtggag gtggcgtagt ctttggacca acaccacgtt
    29281 cttacagcta caaacttcct aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag
    29341 aaaaagttgc tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa
    29401 caaaagaatt caaacaagtt cttgctaact tatctatcga ctctaaagta ttggttgttc
    29461 tagaaaaagg taacgacttt gctgctttat cagcacgtaa cttaccaaac gtttctgtag
    29521 taacttctga taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac
    29581 aaactgctct tactcaaatt gaggaggtgc ttgcataatg aacttactag acgtaatcaa
    29641 acgcccagtg atcactgaaa aatcaatgct tgccatggat gacaagaaat acacttttga
    29701 agtggacact cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg cttttgacgt
    29761 taaagtcaaa aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata
    29821 tgcaggatat acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga
    29881 aatccaatta ttcgaagctg ctgaataagc actaatagat aactcattaa attaggaggg
    29941 aaaaacgtgg cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt
    30001 tctgattttg ctgaaatcac aacatcaaca cctgaaaaat cattgttaca accattgaaa
    30061 aaccatgctg gacgtaacaa caacggccgc atcactgtac gtcatcaagg tggcggacac
    30121 aaacgccaat atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt
    30181 caaacgatcg aatatgatcc aaaccgttca gctaacatcg cgttagtaca ctatgaagat
    30241 ggagtgaaag cttacatctt agcacctaaa ggactagaag tgggtatgaa acttgtttca
    30301 ggtccagaag cagatatcaa agtcggaaat gctcttccat tagaaaacat tccagttggt
    30361 actgttatcc acaacattga aatgaaacct ggtaaaggtg gacaattgat ccgttctgct
    30421 ggtacaagtg ctcaagtact tggtaaagaa ggcaaatacg tattgatccg cttgaactct
    30481 ggtgaagttc gtatgatctt agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa
    30541 caacacgaat taatcaacat cggtaaagct ggacgctctc gttggatgcg taaacgccca
    30601 actgtacgtg gtagcgtaat gaaccctaat gatcacccac acggtggtgg tgaaggtaaa
    30661 gctcctatcg gacgtaaagc accagtttct ccttggggtc aaccagctct tggttacaaa
    30721 actcgtaaca aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa aaataaataa
    30781 ttactttgac catgtgtcgc acattttgag aggaggttca ccatgggtcg tagtttaaag
    30841 aaaggacctt ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc aggggttgaa
    30901 aagaaaaaag ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag ttttgttgga
    30961 tataccatcg cagtttatga tggacgtaaa catgtaccag tttacatcca agaagacatg
    31021 gtaggacaca aattaggtga atttgcacct acaagaactt atcgtggaca tgctgctgac
    31081 gataagaaaa cgaaacgcta atttgagggg aggataaatc caaatggcag aacaaatcac
    31141 atcagctaaa gcaactgcaa aaacagttcg cacttcacct cgaaaagccc gtttagtaat
    31201 tgaccttatc agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa
    31261 caaatctgct ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa
    31321 ctttgactta gatgttgaaa acttggtagt aagcgaagct tttgttaacg aaggacctac
    31381 aatgaaacgt ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag
    31441 tcatatcacg gtagtcgtat cagaaaaata aggagggata atcagtgggt caaaaagtac
    31501 atccaattgg aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa
    31561 aagagtatgc tgagttctta cacgaagatt taagaatccg taaatttatt tcaactaaac
    31621 ttgctgatgc tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt
    31681 caattcacac agctaaacca ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc
    31741 taagaaaaga attaaacaaa ttaactggta aaagaatcca tatcaatatc gtggaaatca
    31801 aaaaaccaga tctagatgca aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc
    31861 gtgttgcttt ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc
    31921 aagggatcaa aactcaagta tctggtcgtc taaacggagc agatatcgct cgttcagaag
    31981 gatactcaga aggaactgtt cctcttcata ctttacgtgc ggacattgat tacgcatggg
    32041 aagaagcaga tacaacatac ggaaaactag gagttaaagt gtggatctat cgtggagaaa
    32101 ttcttccaac taaaaaaaac actgagaaag gagggaaata atcatgttag tacctaaacg
    32161 tgtaaaacac cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga
    32221 agtagcattc ggtgaatacg gtttgcaagc tgttgattca cattggatca caaaccgcca
    32281 aatcgaagct gctcgtatcg caatgactcg ttacatgaaa cgtggtggga aagtatggat
    32341 taaaattttc cctcacaaat cttatactgc caaagcaatt ggggtacgta tgggttctgg
    32401 taaaggggca cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat
    32461 cgcaggcgtt cctgaagaag tagctcgtga agcgttacgt ctagcttctc acaaattacc
    32521 aatgaaaact aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg aaggttaaag
    32581 aaatcagaga attaaccact gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat
    32641 tgtttaatct tagattccaa ttagcaacag gtcaattaga aaacactgca cgtattaaag
    32701 aagtacgtaa atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat
    32761 ggaaggaggc cattaagcgt atgactgaag gaagaaatca acgcaaagtt tatcaaggtc
    32821 gcgtggtttc agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc
    32881 accctatcta tggcaaacgc atgaactatt ctaagaaata caaagtgcat gatgaaaaca
    32941 acacagcaaa agttggcgac atcgtgagaa ttatggaaac tcgtccatta tcagctacaa
    33001 aacgtttccg tttgttggaa gtagtcgaag aagcagttat tatctaataa cacgagattt
    33061 tcctatatag attgaatgca aaatctgaaa ggaggataca caacgtgatc caagcagaaa
    33121 gtcgtttaaa agtcgctgat aactcaggcg cacgtgaaat tttgacgatc aaagtccttg
    33181 gtggatctgg tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg gttaaacaag
    33241 caacgccagg tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa
    33301 aatcaggagc tcgtcgtaca gacggttcat atattaaatt tgatgaaaat gctgcagtga
    33361 ttattcgtga tgataaaagc cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac
    33421 tacgtgaaaa caacttcatg aagatcgttt cactagcacc agaagtttta taatcttgag
    33481 acacttatca aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag
    33541 ttatcactgg aaaagacaaa aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag
    33601 ataaagttat tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg
    33661 ctcctcaagg tggaattgtt gagatggaag cgccaattca tgtttctaac gtgatggtgg
    33721 ttgactctac tggtgtagct ggccgagttg gctacaaaga agtagacggc aaaaaagtcc
    33781 gtgtttctaa aaaaaccggt gaagttttag ataaataaaa gtttaaggaa ggagggtctt
    33841 actgaatgaa ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca ttgatggaaa
    33901 aatttaacta tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg
    33961 gtgttggtga tgcggtatca aacgctaaaa acttagataa agcagttgaa gaattaactt
    34021 tgatcactgg tcaaaaacca atgattacaa aagctaaaaa atcaatcgct ggattccgtt
    34081 tacgtgaagg aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat
    34141 tcttagacaa attggtttct gtttcactac cacgtgtacg tgacttccat ggtgtaagta
    34201 aaaaagcctt tgacggtcgt ggaaactata ctttaggtat taaagaacaa ttgatcttcc
    34261 cagaagtaga ttatgattta gtagataaag tacgtggtat ggacatcgtt attgtaacaa
    34321 cagcaaactc tgatgaagaa tctcgtgagt tgttgacaca attaggcatg ccattccaaa
    34381 aataattaaa ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc
    34441 ctgcaaaatt ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt ccacattcag
    34501 tttatcgtaa gttccatctt tgccgtattt gcttccgcga acttgcctat aaaggtcaaa
    34561 ttcccggcgt gaagaaagct agctggtaaa aagtaaattc gcataagaag gaggtaaaag
    34621 atcaatggtc atgacagatc caattgcaga ttttctaact cgtatccgta acgcaaacat
    34681 ggttaaacat gagtctttag aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat
    34741 cttgaaacgt gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt
    34801 gatccgtgtt ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg
    34861 tatctctaaa ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa aagtattgaa
    34921 cggtttaggt atcgcaatca tttcgacttc tgaaggtgtt gtcactgata aagaagctcg
    34981 tgaaaaaaat atcggcggcg aagttatcgc ttacgtatgg taatcaagta gaatctacaa
    35041 ggaggtgtct ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa
    35101 cagttactca agacggaaac aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta
    35161 ctttttcagc agatatcaaa atgaatatcg aaggaaacga agtaactttc actcgtccaa
    35221 acgacagtaa agaaatgaaa acaattcatg gaacaactcg tgcgaacttc aacaacatgg
    35281 ttgtaggtgt cagcgaaggt ttccaaaaag cgttagaact aatcggggtt gggtaccgtg
    35341 cacaattaca aggatcaaaa cttgtattga acgttggtta ctcacatcca gtagaaatca
    35401 caccaccagc tggagtaact gttgaagtac cagctaacac acaagtaatt gttaaaggtg
    35461 cgaacaaaga agaagttggc gaattagctg caaacattcg tggaactcgt cctccagaac
    35521 cttataaagg taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa
    35581 ctggtaaata atcgtttaga aaaattgcat agaagccagc agcaggaaaa aggcatttgg
    35641 ctgaagttta ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta tttttcgaaa
    35701 cgtatttact gcttgaaata gtagtacaat aaaaataaag aggtgacaat tgtgattaca
    35761 aaaccagata agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa caatatctct
    35821 ggtactgctg agcgcccacg cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa
    35881 attattgatg acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt
    35941 tcaggtggaa caaaaacaga agcagcacaa gctgtcggta aactagttgc tgaacgtgca
    36001 gctgaaaaag gcattaaagt agtagtcttt gaccgtggtg gataccttta ccatggccgt
    36061 gtgcaagctt tagctgaagc tgctcgcgaa aatggactag aattttagaa gaaggaggaa
    36121 taccattcat ggtttatatc gatccaaaac atttggaatt agaagaccgc gttgttgcaa
    36181 ttaaccgtgt aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct gctttagttg
    36241 ttgtcggtga caaaaacgga cacgtagggt ttggtacagg taaagctcaa gaagttcctg
    36301 aagcaatccg taaagcaatt gaagatgcga agaaaaactt agttgaagta cctatggttg
    36361 gttcaacaat cccacacgaa gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac
    36421 ctgctgtaga aggttctggg gttgctgctg gtggaccagt tcgtgccgta ttagaattag
    36481 ctggggtagc agatatcact tctaaatctt taggctcaaa tacaccaatc aacgttgttc
    36541 gcgcaacagt tgaaggtttg aaacaattga aacgtgcaga agaagttgca gaacttcgcg
    36601 gcaaatcagt tgaagaatta atcggataag gaggacaaca ataatggctg aattaaaagt
    36661 tactttaaaa cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt
    36721 aggtcttaca aagattaaca gttcagtggt taagccttct aatgaagcaa ttaaaggcat
    36781 gatcaatact gtttctcacc tagtggacgt tgaagaaatt taatttagaa aaatcataac
    36841 tagaacatta aggaggtgcc aaacctatga aacttcatga attaaaacct gctgaaggtt
    36901 cacgccaagt acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga aaaactgccg
    36961 gacgcggaca aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag
    37021 ggggacaaac accattgttc cgtcgtttac caaaacgagg attcacaaac gtgaaccgta
    37081 aagactatgc agtcattaat cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa
    37141 ctcctgttgc tttagttgaa gctggaatcg tgaaaaacga aaaagctgga atcaaagtat
    37201 tagctaatgg ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag
    37261 ctcaagaagc aattgaagcg gctggtggat caatcgaggt gatctaatgt ttagactatt
    37321 aaaagatgct tttaaagtaa aagacattag atcgaaaatc ctatttactg tatttgtgtt
    37381 gtttgtattt cgcctaggtg cacatattac tgtaccaggc gtaaatgctg cttcattgca
    37441 aaacctttct tctttgccat ttttcaacat gttgaacttg gtaagtggta gtgcgatgca
    37501 aaacttttcc attttctcaa tgggggtttc accatacatt actgcttcta tcgtcattca
    37561 gcttcttcaa atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg
    37621 tcgtaaaaaa ctgaatcaag cgactcgata tctgacatta gtattggcat ttatccaatc
    37681 tgtcggtatc actgctggat tcaatcaata cgctcaatta ggtttcgtga ataatcctaa
    37741 cgtagcaact tacgtaacga tcggtttgat tctaacagcc ggtacgatgt ttgttacttg
    37801 gctaggagaa cagatcacag aaaaaggtat cggaaatggc gtatcaatga tcatttttgc
    37861 aggtatcatt tctagattgc ctgaaagtat cagagaaatt tatgaagatt actttataaa
    37921 tattgatcct tcagacatat ggaaatctgc tatttttgtg gccattttac tagtagcggt
    37981 cttgttgatc gtgacattcg taacattttt ccaacaagct gaacgcaaga tcccgatcca
    38041 atatacgaaa cgcgtagctg gcgcgcctac aagcagctat ctgccgttga aagtaaatgc
    38101 agctggagtt attccagtta tctttgctag ttcgtttatt gctacgccaa atgctgtttt
    38161 acaagcattg agcggtagat tcggttctga aaactggtac aaagtgatga tggagatctt
    38221 taactataat acagcacctg gtgctacgat ttatacgatt ctgatcgttg ccttcacttt
    38281 cttctacgcc tttgttcaag taaaccctga gaaattagcg gaaaacttgc aaaagcaagg
    38341 aagttatata ccaagcgtac gccctggtaa ggggactgaa gagtatgtct caagattact
    38401 gatgcgttta agtacagttg gtgcagtctt ccttggttta gtggcattgc ttccaatcat
    38461 tgctcaaatg gtttgggatc ttccacaatc aatcggatta ggcggtacaa gcttactgat
    38521 cgttatcggt gtagctttag aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa
    38581 atatgttgga ttcattgagt aagaaagcgt gttgaggttt cccctcaaca cacgttctca
    38641 cacatttatt aggagggaaa acaatgaacc tcattttaat ggggctgcca ggcgcaggta
    38701 aaggaacaca agctgaaaaa atcattgatg cttatggtat tccgcacatc tcaacaggag
    38761 atatgttccg cgcagcgatg caaaacgaaa cagctcttgg cctggaagca aagtcttata
    38821 tggataaagg tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa gagcgactag
    38881 ctgaaccaga tacagagaaa ggcttcttat tagatggctt tccacgtacg ctagatcaag
    38941 caaaagctct ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct gtcatcgata
    39001 tccatgttgg tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt
    39061 gtggagcaac ttaccataaa attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt
    39121 gcggtgggca tgaattctat caaagagaag atgataaacc tgagacggtt aaaaatcgtc
    39181 tggctatcaa catcaaaaat agtgaaccaa tcttggctta ttataaagaa caaggtttgc
    39241 taaacactat tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca
    39301 ttgaaaaata gcacgattgc ccgtagccga cttgcattat gcagttactc ttgtcaagtt
    39361 caataaatta tgatagaata tgtcagtccg acttctagag aatcctctag atgatccatt
    39421 ttgagaattt tgaggtggga actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac
    39481 aagtacaagg aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc
    39541 gtcgaaactt tgccgaatgc aatgtttaaa gttgaactag aaaacggaca ccaagttctt
    39601 gctactgttt caggtaaaat tcgtatgcat tacatccgta tcttacctgg tgacaaagta
    39661 acggttgaat tgtctccata cgatcttaac cgtggccgta ttacttatcg ctttaaataa
    39721 ttgtactccg taaaactaca ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa
    39781 tgtgcgaaca ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa
    39841 atccaaaaca taaacaacgt caaggataat tggaggtgct ataaatatgg ctcgtattgc
    39901 aggagtagat attcctcgtg ataaacgtgt agtagtttca cttacttata tctatggtat
    39961 tggtaacact actgctaaaa agatcttagc tgatgctggt gtatctgaag atgttcgcgt
    40021 tcgtgaatta acgaatgaac aaacagatgc tatccgtgca gaagtagata aattaaaagt
    40081 tgaaggggat cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc
    40141 ttaccgtgga atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa
    40201 tgcacgtact cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat aattcttaag
    40261 tgaaggaggt taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa
    40321 gaatatagaa gcaggtattg cacatatcca ttctacattc aataacacta tcatcatgat
    40381 cactgatact cacggaaatg cattagcgtg gtcatcagct ggttcattag gtttcaaagg
    40441 aagcaaaaaa tcaactcctt ttgccgctca aatggcagca gaaactgcta caaaagcagc
    40501 aatggaacac ggattaaaga ctgttgaagt aacagttaaa ggacctggtt ctggacgtga
    40561 agcagcaatt cgttcattac aagctacagg tttagaagtg actgcaattc gtgacgtgac
    40621 tccagttcct cataatggtg cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct
    40681 cattttgagt tttatcagta acgtcattga acaagacact tttcgttttg aaaggggtaa
    40741 agataagaat gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt
    40801 atggcaagtt catcgttgaa cctcttgaaa gagggtatgg gactacttta gggaattccc
    40861 tacgtcgtat tttattatct tctttgccag gtgctgccat cactaatatt caaatcgatg
    40921 gtgtactgca tgaattctct accatcaaag gtgttagaga agacgtcact caaatcatct
    40981 tgaacattaa aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg
    41041 atattaccgg acctgctaca gtaactgctg gcgatattat cgttgatagt gatgtagaaa
    41101 ttcttaacaa agatatgtac atctgtagtg tctctgaagg agctacgttc catgctcgct
    41161 taacagtgaa acctggacgt ggttatgttc aagcagatga aaataaaaaa gaagatatgc
    41221 caattggcgt tcttccagtc gattcaatct acacacctgt tcgtcgtgtg aactatcaag
    41281 ttgagaacac acgtgttgga caccgtgaag attttgataa attgacgatg gagatttgga
    41341 cagatggttc aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac
    41401 atttggatat ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa
    41461 aagaagaaac acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta gacttgtctg
    41521 ttcgctctta caattgtttg aaacgtgcag gaatcaatac tgtacaagaa cttacaaata
    41581 aatctgagcc tgaaatgatc aaagtccgta acttaggacg taaatcatta gaagaagtta
    41641 aagcaaaatt gcacgactta ggtttaggtt tacgtaaaga agattaaaac cttttacgaa
    41701 ggagggaaac caacgtgagt tatcgtaaac taggacgcac atctagccaa cgtaaagcga
    41761 tgttgcgtga tttaacaact gatttattaa tcaacgaacg tattgtgact actgaagctc
    41821 gtgcgaaaga agttcgctca actgcagaaa aaatgatcac tttaggtaaa cgcggagatt
    41881 tgcatgcacg tcgtcaagct gcggctttcg tacgtaatga agttgccagc gtgcgtgaag
    41941 aaaatgagga catcgtcatt gaatcagctt tacaaaaact gttcaacgat attgcacctc
    42001 gttacgcaga acgtcaaggc ggatacacac gcattttgaa gacagaacca agacgcggtg
    42061 atggtgcacc aatggttgtc ttagaacttg tctaaaatca cactaataac atcactttat
    42121 agatgattct tttagagtgt tatgatgttg gaagatattt ttccgagtct agctcagcgc
    42181 tgctcctcaa gcgaatatcg cttggggagt aagcactcat cataaagtgt ttttttatac
    42241 cttcaattac ctgtcatgac agatatgttt gaaggcataa aaaaacattt taagcgagaa
    42301 ataattgaaa attttgtctt tttttgatag tgttagtagg aactttagga tgaagaagga
    42361 gtaatcatgg agccaattat tgaattagaa aagataaatt ataaatacca gccagatgat
    42421 cttcgccccg cattgaaaga tgtctccttt acaattgata aaggtgagtg gatcgctatt
    42481 attggacaca atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg
    42541 ccagaatcag ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa tatttggacg
    42601 atccgacaga tggttggaat gatttttcaa aatccagaca atcagtttgt tggttcgaca
    42661 gtagaagatg acgttgcttt tggtcttgaa aatcaaggga ttcccagaga agaaatgcta
    42721 gtgcgtgtga aagatgcact tgagaaagtt agaatggctg agtttgcttc acgcgagcct
    42781 gctcgtttat caggagggca aaaacaacgt gttgccattg ccggcgtggt tgccttacga
    42841 cctgatatca ttattttaga tgaagcaacc agtatgctag atccagaagg tcgtgaagaa
    42901 gtgatctcta cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat atctatcact
    42961 catgatattg atgaagcagc taatgccaat cggatactag tgatgagaca aggcgaactt
    43021 gtccgtgaag gtacaccaaa agaaattttt tctgctggtc ctgaactgat cgacttaggt
    43081 ctagatctac catttcctga aaaactgaag agcgcactga aagaacgtgg ggttgatgtg
    43141 cccagtgaat atatgacaga agaaaggatg gtggattggt tatggacatc cgttttgaac
    43201 aagtagactt cacttatcag ccgaatacac catttgaaca aagagcctta tttgacatca
    43261 atatgacgat caaagaaaac agttatactg cattagtagg gcataccgga agcgggaaat
    43321 caaccttact tcagcatttg aatgcgcttg ttaagccaac aagcggtaca gtccatattg
    43381 gagaacgaga tattcagccg gatacggata ataagaattt aaagcccatc cgaaaaaaag
    43441 ttggcatcgt ctttcagttt ccagaagcac agctattcga agaaacggta gcaaaagaca
    43501 ttgctttcgg tcctaaaaac tttggtgtca gcgaagaaga agcactagtc ctagcaaaag
    43561 aaacattaga acaagttggg ctggatgaaa gctatttgga acgttcgcca tttgaacttt
    43621 caggagggca aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac
    43681 ttgtattgga tgaaccaacg gcaggacttg atccccaggg cagaaaagaa atgatggaga
    43741 tgttttggcg tcttcataag gaacagcaga tcactattgt attagttacg catttaatgg
    43801 atgatgtagc caattttgca gattatgtct atgtgttgga aaagggacga atcgttaata
    43861 gcggggagcc gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg
    43921 tacctactgc caccgagttt gcagaagaat taatggctaa agggatgaac tttgctacct
    43981 tgcctttgac agctgaagaa ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc
    44041 atgatgaata aattgatatt tggtcgttat atgcctggag attccttgat ccatcgattg
    44101 gatccccgag ctaaattatt agccagtttt tattttatcg gaatcatttt tttagcaaat
    44161 aattgggaaa cgtacctttt gttggcattt ttcacattac tttctgtctt cttgtcaaaa
    44221 gtagatttga aattttttat ccgaggcatt cgcccattga tctggctaat cctcttcact
    44281 gtagctttgc aggtcttgtt tacaagtggc ggagaaactt attggagttg gggtgttttc
    44341 catatcactg aatttggtgt gatgaacggc ctctttattt tctgtcgatt cgtgttgatc
    44401 atttttatgt cgactctact cacgcttact acaccgcctt tggaattatc ggatgcgatt
    44461 gagtacttgc ttcgtccatt gaaagccgta cggttcccag tccacgaaat ttcattgatg
    44521 ctgtcgattg ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac
    44581 gctcagcgcg ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa aatgaaagca
    44641 gtcattcctt tgctgattcc tttatttgtc agcagcttca accgtgcgga agatcttgcg
    44701 actgctatgg aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg
    44761 cattggcata atagagacac gatggttatc attgcttttg tattattaac tgtgctactg
    44821 atctttttaa gaggataaac gttcagtggg ccgctatcta ttcttttctt gctgatctgt
    44881 ttgcaagaaa ggatgcaagc agtgtatgac aagaaatatt agggtctgtg gcagactaag
    44941 gtcacaggcc tctttctttt atctgaaaag aaagaacgaa aaaattttat agaaaaatca
    45001 ttattccaga taacctttta ggaacaaagg agagaaaaac atggtacgtt acaaagcaat
    45061 cattgcgtac gacggaacaa attttaatgg attccaaaaa cagccaaatg ggcgtactgt
    45121 tcaagaagaa gtagaaaaaa cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt
    45181 tggttcggga aggacagatg ccggagtaca tgcgatcggg caagtaatcc attttgatta
    45241 tccagaggag cgtccgttag aacgaatgcg tttcgcgcta gatacccagt caccagagga
    45301 tattgctgta agacaagtag aaattgtttc agaagacttc cacgcaagat atttggtcaa
    45361 agaaaagacg tatcaatttc gagtggatat agggaaacca cgcagtcctt ttcgacgcca
    45421 ttatgcaagt tattttcctt acccacttga tttggaaaaa attcagcggg cgcttcctga
    45481 tttattaggg actcacgatt tcacctcttt ttgtgcatca ggaagctcga tagaggataa
    45541 aatacgtacg atctacgagg ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt
    45601 tacatttagg ggaaatggct tcttgtataa gatgatacgg atcttagtag gaacgctttt
    45661 gaaaattggc aatggccgat tgcctgaaga tagtatccca gatattattg cgaaaaaaga
    45721 tcgtaatgca gcaggaccta cagcacaccc tgaaggactt tatttgtatg aagtgaaata
    45781 tgaataagag gttatgatag aagtggtcag cttcaagaaa taagaaggta tttacgaaaa
    45841 ttacttctca aaattttgca aaatttcggc ttattttcga agaagctact tctggaacac
    45901 cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaaaga gcaatttcaa
    45961 aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa
    46021 tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg
    46081 cccttggtgc ggacagtctg aacaggttcc atcgtttctt tattttctac gtcttcttct
    46141 gtgtgtgttt cgtggttgtt cttctattgc tggatcaatc gtgccataaa aggggcgatt
    46201 gcggtcaagg taccagacat aaagaataaa caatgcaata cataggacaa aaaggattgc
    46261 acctatcgta aatccttcag gcaagtaact gaaagtgaca gtatgtttac ctgctggaac
    46321 ttttatcatc aagaatgcgt ctttgaaatt gctgaccttg actggtttcc catcaatttt
    46381 agctttccat cctttgtcat aaggaatagt tgtcaccaac atggaatctt cagcggtatt
    46441 tacagtccca gaagcttttc tgccagtggc ttccatttcg acaccttttt ctttgatctg
    46501 attcattgcg gcttgatagg ctacaacatc tagaccaacg actttaggct ccatgaaact
    46561 tatttctttc gtcccataaa aacttgcagt aaatgtcact gttgtctctt gaggataata
    46621 gccaagatca tagtattgac cagaaatgtt gatttgcgtt tttcgcgatg taccattgac
    46681 agtgatcgtc gcactagaac tttctaattg cgcaaaattg gttgggaaca aacttaagta
    46741 agcttgtgta tttgcaggca catggacttc ccaagtgagc tcttttggct ggttatctgc
    46801 tgtttctgag taagtgacgc cagtcgttgt ctttgtgatc gtcgtattca actgattctt
    46861 caaagtaggt gtatagaatg taaaatactg ttcatttaga ccagacaatg tattgatcaa
    46921 gtttgtctga ctggtcaaat tatctgtcac tggctgctca acatcattaa cggacaacgg
    46981 tgcagtaaat gctagtggca atgcattgac attctgatac agtgtatagt cgccgctttg
    47041 accagcttgg cggaagccat atttactgaa cgtgctttcg tttttagaaa tgttatattt
    47101 gattcctgta aacccatcca tcagcaaagt attattgtta taacggatat tcaaatttgt
    47161 ccctcttgaa cggaaaccta atttatccag gtaagaggaa gaattacgat ttcttataga
    47221 agaaaacaaa ctgataccac tgtaaccata gttgatcgaa tcgtttgcag atactggatc
    47281 cagattttcc aagcggtaaa acgagtcgga atcttttttt gtttcattta caagagcttt
    47341 caagtcagga taaggatcag tatataaact gcgggaagca taattccaat catcaagaat
    47401 gccatggatc atactgttcg tattgaccag tgcttcagca gacatgatca ataataacaa
    47461 tatactcaga tgattgaatg aaagtttttt atgctgataa aatcccacag caaaagcata
    47521 aatccctaag aaaaccactg tcaatacaaa ggaagtcatc gtcacatagg tgtagcttcc
    47581 ttcaggtttg atgccccatg ccaccgcaaa gagagcagct acgaccaaaa tgattcctaa
    47641 caacagccca ccatcgtctt gttctatctt ttcccatcct tttcctgcaa gcaggagcac
    47701 taagaaagag aacagatagg cataacggaa taaaaacata ttcggtgcat gcataccatg
    47761 ccagaacaaa ttcaatggaa caagataaaa acttgcgata agtatggcga aaagactgcc
    47821 gtacaaaaat ttatttttca acggtacttt ccgactggca aaataaaaca gacagaggac
    47881 taatggtagc agacctgcgt aaacgaacgg aatcgatccg tattttgtcg tgtcataaac
    47941 accaatcata tttttcatga ttagatccag ataagaagtc gcttctgttt tcaacgtagt
    48001 gatctgactc agtgcctcgc catttgaccg aagatccagc actgctggca atacaatgat
    48061 cattgaagcg ccgcctgcca gtaaagaagt gataccataa ggaacaatcg tttttttata
    48121 ttctgaccag tttgtcaata gacgggcgat aaaatataag aaagaaaata cgccaatcat
    48181 aaaacccata taaaaactgg ataaaaacaa gagtaaataa ctgataaata aaagtttcgg
    48241 ccgtttaaga tccataacac gatgaatccc caaaatcact aatggaagat aaacaaatgc
    48301 atccagccac atgatgatct ccgaatgtgc agtgataaat gagatcaatg cgtaactgac
    48361 gctaagtgca acatgccccc agcgaggaat cttgaacgta cgtgcaagaa accaaaaagc
    48421 caatccggca cagccgacct taagcaaagt cagaacatac agtgcatctg gcatcatctg
    48481 gtttggaaaa aagaacacca gtggcgtgaa gatcccgcct agatagtaag aaattaaaga
    48541 taaataattc aatcctagag aagcattcca tgtataaaaa atactttgct ttccaagaag
    48601 aacatttcgg aagctggcat gaaaattaga aaactgtgaa aaggcatcac ttgccaaaat
    48661 acttctgctg cttccaggat agattccgat acttagataa acaagtgcta aaatcaaaaa
    48721 cggaatcagg aaactagctg ctgcgtagcg tccgttttct ctaaagaatg actttaaatt
    48781 ttttttcata tttattttcc accaaatttc tatgaagttg tattcagttt atcatatcct
    48841 tgtttttctc tgcttaaaaa gagtctagtt ttcacaaaaa aataaaattt agaataaata
    48901 acttcataat ttgttcgtat aacaaaaata aacgcaaaaa aagggtagga atcaaaagtc
    48961 ccacccttaa aaatttttat taaataaaaa accgttcact atctgaacgg tccaagtatc
    49021 attcaattaa acgcgttcaa cttttcctga tttcaaagca cgagttgaca cccaaacttt
    49081 tttaggtttt ccgtcgacca taacgcgaac tttttgtaag ttaggtttaa cagtacgttt
    49141 tgttgagttc atagcgtgtg aacggttgtt tccacttttt gtcttgcgac ctgtaaagta
    49201 acaaactttt gccatggtaa tttcctcctt tgccttgatt tggagcaata gaataattca
    49261 gctcatacca gactaacata tcatatagaa tgggaatttg caagtacatt ttcatagata
    49321 caattgacaa ttgagaagaa taattgtaaa ttaaacaagg ttatacctga aattttgcaa
    49381 ggtttttgag tactttgggt aactgccttt tatttcaagt tgcgctatga taaaatatga
    49441 tagaagaaaa atgggagttt tttacccgtt aaggaggctc attcatggct gtaaaaattc
    49501 aaacgtcagc tggaaccatt gaaattacca atgatgtaat tgcaacagtt gttggtggcg
    49561 ccgcgacaga tgtgttcggt gttgtaggaa tggccagcaa aaatcaaatt aaagataata
    49621 ttactgagat tttgcgtaaa gaaaattact caagaggagt agttgtccgc caagaagata
    49681 atggtattgc tgtagatgtt tatacgatta tcagctatgg tacgaaaatc tctgaagttt
    49741 cccgcaacgt ccaagaaaaa gtgaaataca atctagaggc attattaggc gtaactgcca
    49801 attcagttaa tgtctttgta caaggtgttc gtgtactgcc agactaggca ggagactggc
    49861 tggtaactca gcagtcgatc tgaaggagga ttatctaggt gagtgtaaca gaaataagtg
    49921 caagccagtt ccaggaaatg gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat
    49981 acgtcaattc attaaatgta ttccctgtgc cagatggcga tacgggaaca aatatgaatc
    50041 tatctatgac tagtggagct aaggcagtag tagattcaac ctcagaaaaa gtaggagaat
    50101 tagcagcaat gctttcaaaa ggattgctga tgggtgcgcg cggaaattcc ggcgttattt
    50161 tgtctcaatt gtttcgtgga ttttctaagc aaattccaga tgttgtctca ttaaatgctt
    50221 ctgatcttgc tgcagccttc actcatggag ttgaaactgc gtataaagca gtaatgaaac
    50281 cagtagaagg aacgattttg acagtagcta gagaagcagc gaaagctgga gaaaagaaag
    50341 caaaaagtac tgatgatgtc gttgaagtaa tgacagctgt tgtaaaagga gcaaagcgtg
    50401 ctttagctaa aactccagac ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg
    50461 gagggcaagg actgctgttt gtttatgagg gctttttaag tgcattgaat ggagaattcc
    50521 aagcggatga aacatatgaa ccttctccag cagaaatgga tgatatggtc aatgcagaac
    50581 atcatcgcag tgtccaaggt caaatggcga cagaagatat ccattttggt tactgtacag
    50641 aaatcatggt aaaaatcgga gaaggaccta cagtcgatag tacattcgat tacgaagaat
    50701 tccgtaacta tttagatggt ataggagatt ctttacttgt ggtcaatgat gacgagatca
    50761 tcaaagtcca cgtgcataca gaaaacccag gagaagtctt gaattatgga caaaaattcg
    50821 gttcattagt caaaatcaaa gtcgacaaca tgcgcttgca gcatgagacg attttggaac
    50881 acgatgaaga agtgaacgag tttgcgaaaa aagaaatacc acgtatcccg tatggtatcg
    50941 tagcgatcgc tgctggtaaa ggcgtccaag agctgtttga aagtttaggc gctaattatg
    51001 ttatcagtgg tggacaaaca atgaatccaa gtacggaaga cattgttaaa gcgattcaag
    51061 aagtaaacgc agataaagtg attgtcttgc caaacaataa gaacatcttc atggcagctg
    51121 atcaagcagc agaagtagca gatatcccag tagcagttgt tccttcaaga acgatttccc
    51181 aaggattgac agctatgctt tcattcaatg atcaagcttc tttagaagaa aacaaagaaa
    51241 cgatgacaga aatgttgtct agcgttgtga gcggtcaagt cactcacgcg atcagagata
    51301 cctcaattga cggtgtagag atcaaagaag gagacttttt gggcatgatc gatggaaaaa
    51361 tcctgatctc taacccagat atcacagcaa cagcgttaag tacactagaa caaatgatca
    51421 atgaggacac agagatcgta acgatcttgg caggtgatga aggaacagtt gaacaagcaa
    51481 atgaatttgc tgagaaacta acagaaaaac atcctgattt ggaaatcgag atccaccaag
    51541 gagatcagcc tgtctatcct tatctatttt ctgctgaata atttctgtgt cagaatatct
    51601 ttctaacaca gtattcattc ataaaaggcc ttcggttagt gatcaggtat gatcgctgac
    51661 caagtccttt ttttgttaaa ggagggagga aaaatttatg cgttctttag atgatccaat
    51721 cactatgctg gcaggagtcg gagaaaaaag agcagctagt cttgccagct tgggcatcca
    51781 aacgattgaa gacttactga cttattatcc ttttcgctac gaagatattc aggaaagaaa
    51841 tctaaacgag atccaggatc aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc
    51901 tgtgatgaat cgatttggtt ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca
    51961 tgatgtgttc aacgtttctt tttttaatca accttatcta aaagaaaaag tcattttatc
    52021 ggaggaaatc gccgtatacg gtaaatggga tgcaaagcgt aaagccttga acgggatgaa
    52081 gattttaggt tctcaaacaa tggatgactt ttcgccgata tatcacgtca acaaatcaat
    52141 tcgtcaaacg acattagttg atctaatcaa aaaagggttt caagaatacg gcacattgat
    52201 tgaagaaaat ctgccaaaca accttgtgga gaaatatcga ctgcttgacc gagcttctgc
    52261 tgtaagaagt atgcattttc caaaagacca cgaagaaaac catcaagcca aacgacgtgt
    52321 cgtttttgaa gagttctttt tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa
    52381 agcagaaacg aatggtttgg agatccttta tgatgtccaa aaattgaaac aatttactca
    52441 aaagctacct tttgaactga caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga
    52501 tcttcgcagt ccgcagcata tgcagcgact tttgcaggga gatgtaggta gcggaaaaac
    52561 agtggttgcc gcgattgcgt tatacgctac cgttacagca ggtttccaag gtgcgttgat
    52621 ggtgcctaca gaaatcttgg cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc
    52681 caatgaagta accatcgcat tgattacggg gtcaacgaaa gcaaaagaac gacgtgagct
    52741 ccttgaaaaa ctagagcaag gggaaattaa cgtcgtgatc ggcacacatg cattgatcca
    52801 agatggagtc gaatttcaga atttaggtct agttattacg gatgagcagc accgttttgg
    52861 cgtgaaccag cggaaagtac ttcgtgaaaa aggctggcga ccagatgtct tatttatgac
    52921 agcgactccg attcctagaa cattagctat tacagcatat ggtgagatgg atgtatctat
    52981 cattgatgaa cttccagctg gacgtatccc gatcgaaaca cgctgggtaa gaacacctca
    53041 attggacagt gtactggatt ggacgaaaaa agaactggca agaggacatc aaatgtatgt
    53101 catctgtccg ctgattgaag aatcagaagc attagatgta aaaaatgcag tggaaatcta
    53161 tgaaaaactt cgtgaactat ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat
    53221 gaagaatcag gaaaaagaag taatcatgga agccttcaaa gacaaccaga tgcagatatt
    53281 agtttcgact actgtaattg aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat
    53341 catggatgcg gatcgatttg gattggcaca gctgcaccaa cttcgtggac gtgttggacg
    53401 cggaagtgat gcttcctact gtatcttagt tgctaatcct aaaaatgaac taggtgtgga
    53461 acggatgaaa atcatgacag agaccaccaa tggttttgta ctaagcgaaa aagatctgga
    53521 attacgtggt cctggtgaag tatttggctt ccgtcaatct ggtctgcctc aatttgcgat
    53581 tgctgacttg gtgacggatg gaaatgtttt ggaggtagcc agagaagagg ctgcagccct
    53641 ttggcaaata aaggattggc agatgcttcc tgagtatcag cctttagcgc aaagcctcca
    53701 aatagaaaat caggataatc gttatttcga ttagcagact gtgctatact ttatactgat
    53761 gacggaaaaa ggagtggaaa caatgaaaat tgccgtagat gcaatgggcg gagatcacgc
    53821 acctcaagca attgtagaag gcgtggcttt agcgcagcaa gattttccag aagtagaatt
    53881 cttactttac ggaaaagaag cagaaatcaa gaaatactta acaaatgaaa aaaacatcac
    53941 gattatccat acagacgaaa aaatcaatag tgatgatgag ccagtaaaag caattcgacg
    54001 aaaaaagact gcatcaatgg tccttgctgc acaagcagtg aaaaacggtg aagcagatgc
    54061 tgtattttca gcagggaata caggcgcgct actagcagca gggttgttca tcgtaggacg
    54121 tatcaaaaat atcgaacgcc ctggcttgat gtctaccttg ccagtagtag ggaaagaagg
    54181 tgccggtttc gatatgctgg atcttggcgc taacgcagaa aacaaaccag aacatttgtt
    54241 gcaatacggt attttaggtt ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg
    54301 tgtcgcactc ttaaacaacg gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc
    54361 ctatgaactc cttcagaatg aaacaagctt gaattttatt ggaaacgtag aagctcgtga
    54421 gctactcaat ggtgtggctg atgtagtggt cacagatgga tttacaggca acgctgtttt
    54481 gaaatctatt gaaggaacag caatgaatat gatgaatcta ttaaaatctg ctattctaaa
    54541 tgaagggatc aaaggaaaaa tgggcgctat gcttttaaaa gacggcttac gttctttgaa
    54601 agcagaaatg gattattcca aacatggcgg tgccgttttg tttggcttga aagcaccagt
    54661 cattaaaact catggagcaa caggccccga tgctgttcgc tatacgatcc gccaaatcca
    54721 cacaatgctt gagacagatg tagttggaca attagtcgaa caatttgaaa aaagcgaaga
    54781 atagtatttg gatatctgaa gcgatccgtt ccattgaaaa aaaggtggac attcctgaaa
    54841 aatgccagta aaattagcca tggtgtaggt aattgacaag tgtatgattc ttggaggtga
    54901 aaaagttgac tcgcgaagaa gtatttaaca aagtagcgaa aattatttca aatcattttg
    54961 agatagatac cgataaagtg acggatgaat tgaacatcaa agatgattta aaagctgatt
    55021 caattagtat tatggaattc gttttagagc ttgaagatga attcggtacg gaaatttcag
    55081 atgaagacgc tgaacaaatt gaaacagtcg gtggcgcagt agattatatc agcagccatc
    55141 ttaaataatt tcaaataaaa gcaaagaagg ataaaaagct gcatgcagct ttttatcctt
    55201 ctttttttaa tctttttgac acgtaaaagt catttttctg ataat
//
LOCUS       DARBER010000007        49191 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARBER010000007 DARBER010000000
VERSION     DARBER010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.007"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            129..941
                     /locus_tag="SCK06_000465"
     CDS             129..941
                     /locus_tag="SCK06_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG1150496.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            938..1738
                     /locus_tag="SCK06_000466"
     CDS             938..1738
                     /locus_tag="SCK06_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG1150497.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1899..3167
                     /gene="rseP"
                     /locus_tag="SCK06_000467"
     CDS             1899..3167
                     /gene="rseP"
                     /locus_tag="SCK06_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG1150498.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3235..4944
                     /locus_tag="SCK06_000468"
     CDS             3235..4944
                     /locus_tag="SCK06_000468"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG1150499.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5156..9514
                     /locus_tag="SCK06_000469"
     CDS             5156..9514
                     /locus_tag="SCK06_000469"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG1150500.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9659..10132
                     /gene="rimP"
                     /locus_tag="SCK06_000470"
     CDS             9659..10132
                     /gene="rimP"
                     /locus_tag="SCK06_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG1150501.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10155..11330
                     /gene="nusA"
                     /locus_tag="SCK06_000471"
     CDS             10155..11330
                     /gene="nusA"
                     /locus_tag="SCK06_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG1150502.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11352..11645
                     /locus_tag="SCK06_000472"
     CDS             11352..11645
                     /locus_tag="SCK06_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG1150503.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11642..11953
                     /locus_tag="SCK06_000473"
     CDS             11642..11953
                     /locus_tag="SCK06_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG1150504.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11966..14272
                     /gene="infB"
                     /locus_tag="SCK06_000474"
     CDS             11966..14272
                     /gene="infB"
                     /locus_tag="SCK06_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG1150505.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14299..14646
                     /gene="rbfA"
                     /locus_tag="SCK06_000475"
     CDS             14299..14646
                     /gene="rbfA"
                     /locus_tag="SCK06_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG1150506.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14803..14976)
                     /locus_tag="SCK06_000476"
     CDS             complement(14803..14976)
                     /locus_tag="SCK06_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1150507.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15424..17786
                     /locus_tag="SCK06_000477"
                     /pseudo
     CDS             15424..17786
                     /locus_tag="SCK06_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18154..19076
                     /gene="truB"
                     /locus_tag="SCK06_000478"
                     /pseudo
     CDS             18154..19076
                     /gene="truB"
                     /locus_tag="SCK06_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
     gene            19080..20021
                     /gene="ribF"
                     /locus_tag="SCK06_000479"
     CDS             19080..20021
                     /gene="ribF"
                     /locus_tag="SCK06_000479"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG1150508.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20373..21158
                     /locus_tag="SCK06_000480"
     CDS             20373..21158
                     /locus_tag="SCK06_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1150509.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21171..22022
                     /locus_tag="SCK06_000481"
     CDS             21171..22022
                     /locus_tag="SCK06_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG1150510.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22146..22598)
                     /locus_tag="SCK06_000482"
     CDS             complement(22146..22598)
                     /locus_tag="SCK06_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150511.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(23063..23431)
                     /locus_tag="SCK06_000483"
     CDS             complement(23063..23431)
                     /locus_tag="SCK06_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG1150512.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23581..24063
                     /locus_tag="SCK06_000484"
     CDS             23581..24063
                     /locus_tag="SCK06_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG1150513.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24100..25110
                     /locus_tag="SCK06_000485"
     CDS             24100..25110
                     /locus_tag="SCK06_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG1150514.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25225..25938)
                     /locus_tag="SCK06_000486"
     CDS             complement(25225..25938)
                     /locus_tag="SCK06_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150515.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26128..26718)
                     /locus_tag="SCK06_000487"
     CDS             complement(26128..26718)
                     /locus_tag="SCK06_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG1150516.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27202..27429
                     /locus_tag="SCK06_000488"
     CDS             27202..27429
                     /locus_tag="SCK06_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG1150517.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27445..28611
                     /locus_tag="SCK06_000489"
     CDS             27445..28611
                     /locus_tag="SCK06_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG1150518.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28845..29888
                     /gene="hrcA"
                     /locus_tag="SCK06_000490"
     CDS             28845..29888
                     /gene="hrcA"
                     /locus_tag="SCK06_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG1150519.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29982..30545
                     /gene="grpE"
                     /locus_tag="SCK06_000491"
     CDS             29982..30545
                     /gene="grpE"
                     /locus_tag="SCK06_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG1150520.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30596..32425
                     /gene="dnaK"
                     /locus_tag="SCK06_000492"
     CDS             30596..32425
                     /gene="dnaK"
                     /locus_tag="SCK06_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG1150521.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32576..33742
                     /gene="dnaJ"
                     /locus_tag="SCK06_000493"
     CDS             32576..33742
                     /gene="dnaJ"
                     /locus_tag="SCK06_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG1150522.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33824..34489)
                     /locus_tag="SCK06_000494"
     CDS             complement(33824..34489)
                     /locus_tag="SCK06_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150523.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34465..34638
                     /locus_tag="SCK06_000495"
     CDS             34465..34638
                     /locus_tag="SCK06_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150524.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34683..35420)
                     /locus_tag="SCK06_000496"
     CDS             complement(34683..35420)
                     /locus_tag="SCK06_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1150525.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35422..36924)
                     /locus_tag="SCK06_000497"
     CDS             complement(35422..36924)
                     /locus_tag="SCK06_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG1150526.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37183..39177
                     /gene="uvrB"
                     /locus_tag="SCK06_000498"
     CDS             37183..39177
                     /gene="uvrB"
                     /locus_tag="SCK06_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG1150527.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39187..42003
                     /gene="uvrA"
                     /locus_tag="SCK06_000499"
     CDS             39187..42003
                     /gene="uvrA"
                     /locus_tag="SCK06_000499"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG1150528.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42266..43531
                     /locus_tag="SCK06_000500"
     CDS             42266..43531
                     /locus_tag="SCK06_000500"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG1150529.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43535..44266
                     /locus_tag="SCK06_000501"
     CDS             43535..44266
                     /locus_tag="SCK06_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG1150530.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44401..45285
                     /gene="rapZ"
                     /locus_tag="SCK06_000502"
     CDS             44401..45285
                     /gene="rapZ"
                     /locus_tag="SCK06_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG1150531.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45282..46280
                     /locus_tag="SCK06_000503"
     CDS             45282..46280
                     /locus_tag="SCK06_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG1150532.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46314..47249
                     /gene="whiA"
                     /locus_tag="SCK06_000504"
     CDS             46314..47249
                     /gene="whiA"
                     /locus_tag="SCK06_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG1150533.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47792..48442
                     /locus_tag="SCK06_000505"
     CDS             47792..48442
                     /locus_tag="SCK06_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150534.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48629..>49191
                     /locus_tag="SCK06_000506"
     CDS             48629..>49191
                     /locus_tag="SCK06_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG1150535.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16471 a         8421 c        10576 g        13723 t
ORIGIN      
        1 taaaaattaa aaaaagaaaa aattattcag tttttaaata aaaaaacaat gatttcatag
       61 gaaaaacagt tagatattgc tataatactt aagatagtgg gaatctttta agtataggag
      121 gaaaaagaat gttacgtttt tttccgcaaa aaaacaaata tattcaagaa aaaagcgaat
      181 ttcaatttaa taccgatgga ccaattcctc agcacattgc catcatcatg gacggcaatg
      241 gtcgttgggc gcaaaaccgt cgtttgccac gagttgctgg ccataaagaa ggcatggaaa
      301 cagtaaagaa agtcacaaaa aaagcatcaa ggcttggagt gaaagtgttg acgctttatg
      361 ccttttcgac tgaaaactgg aagcgaccaa aagatgaagt aagttttctc atgcaactac
      421 ctgtcgactt ctttgatacg ttcgtaccag aattgattaa agaaaatgtc aaagttcatg
      481 tcatgggtta tgaaaacgta ttgccagaac atacgcaaga tgctgtaaga cgtgcaattg
      541 agcaaacaaa aaacaatact ggaatggtat tgaattttgc tttgaattat ggaagcagag
      601 cagagatcgt gactgctgtc caagaaattg ccgaagaagt agcaaaagga gaaatacacg
      661 cagaagaaat cgatgatgaa ttgattgcta agcatttaat gacaggtttc ttgcctaaag
      721 agttacaaga tcctgagctg atgatccgta caagtggaga agagcgaatt agtaattttc
      781 tgttatggca gatcgcatat agtgagctgt attttacaaa agcgctttgg cctgattttg
      841 atggtgcgca tcttgaagaa gcaatcgcct cttatcaaaa tcgagaccgt cgattcggcg
      901 gtgtaaaaaa aacagaagat gagaaagggg atcagtcatg aaacaacgtg tggtcacagc
      961 agccgtggcg ctagtcttgt ttattccgat tatatggatc ggaggcatag cggttgagct
     1021 tacagcagca atcttagcag taataggagt ttacgagctg tttcggatga aaggattgac
     1081 actcttaagt tttgaaggag ttttatccgc cgttggtgca gtgttacttg ttttgccaaa
     1141 agaaagatgg tttttctttc ttccagagaa tgtaagtaca tttatgttat tttatcttat
     1201 agttatgatt cttttaggaa catcagttat ttctaaaaat acgtatacga ttgatgaagc
     1261 aggttttcct gtgcttgtca gcttgtatgt gggaataggt ttccagaatt ttgtcaatgc
     1321 acgaacagaa agcttggctg ttttgatatt tggtctattt attgtttggg caactgatat
     1381 cggagcatat atggttggtc gacgttatgg tcagcataag ttaagtcctg atatttcgcc
     1441 caacaaaacg attgaaggtg cgctcggtgg tattggtagt gctgttgtag tcgcatttct
     1501 ttatttattg ctatacccag caaaagaact atttaatcat ggtatgtttg tcatgcttct
     1561 gatgacgatt attttttcag cagtcggaca atttggtgat ttggtcgaat ctgcaatcaa
     1621 acgtcattat gatgtgaaag actcgggaaa tatcttacca ggccatggtg gaatcttaga
     1681 ccgtttcgac agtttgctgt ttgtatttcc tatcatgcat ttatttggca ttatttagta
     1741 agtatacctg cgtttctttg tgaatttcat aaaagacgca ggtttttatt tttcattata
     1801 ttgatactat tggtgtcttt ggtatactat ggtaaaatta tagtgtttta tcgttgtttt
     1861 ataaaaaaca caaaaaacaa gtaaaggaga aaaaagctat gaaaacgatt ctgacattta
     1921 tcatcgtttt tggtatatta gtgattgttc atgagtttgg tcatttcttc tttgcgaaac
     1981 gatcaggaat cctcgttcgc gaatttgcta tcggtatggg tccgaaaatt tatgggcatc
     2041 aagcaaaaga cgggacaaca tatacgcttc gcctattacc tattggcggt tatgttcgaa
     2101 tggcaggcaa tggggatgat gaaacagaaa tggcaccagg aatgcctctt tctcttttac
     2161 taaattctga cggcatcgta gagaagataa atttaagtaa gaaaatccaa ttgacaaatg
     2221 ctatcccgat ggaattgagc cgttatgatt tagaagatga gctgactatc actggttatg
     2281 tgaatggaga cgaaacagaa gtagtaacct accctgttga tcacgatgcg acgattattg
     2341 aaaacgacgg aacagaaatc agaattgcgc caaaagacgt acagttccaa tcggctaaat
     2401 tgtggcagcg tatgctgacc aactttgctg gacccatgaa taactttatt ttggcaatcc
     2461 tgttattcat cattctggca tttatgcaag gtggtgttca agtgacgaac actaatcgcg
     2521 taggagagat catgcctaat ggagctgcgg cagaagcagg cttaaaagaa aatgatgagg
     2581 tagtcagtgt ggacggaaaa gaaatccatt catggaatga tctgacaaca gtcattacga
     2641 aaaatccgga taaaacatta gactttaaga tcgaacgaga aggtcaggtg caatctgtcg
     2701 acgtgacacc gaaatctgta gagtcaaatg gcgaaaaagt tggccaactt ggtataaaag
     2761 ccccaatgaa tacaggtttt atggataaga tcattggagg gacgcgtcaa gctttcagcg
     2821 gttcattgga aattttcaaa gccttaggat cactatttac aggtttcagt ttagataaat
     2881 taggcggacc tgtcatgatg taccaattgt cttcagaagc agcaaatcaa ggtgtaacaa
     2941 ctgttattag tttgatggcg cttctttcaa tgaatctcgg aatcgtcaat ctgcttccga
     3001 ttcctgcctt agatggcggg aaattagtct tgaatatttt tgaagggatt cgcgggaaac
     3061 cattaagtca agaaaaagaa ggaatattga cattagctgg ctttggattt ttaatgttgt
     3121 tgatggtgct agttacatgg aacgatattc aacgattctt tttctagaaa aagttgacaa
     3181 atagggtata ataacaaacg ataactattt tgaggaaata aaggagtata tagaatgaga
     3241 caatcaaaaa tgttgattcc aacattacgc gaagttccaa atgatgcaga agtattaagc
     3301 catcaaatct tgctaagagc tggatatatc cgccaagtat cagcgggtat ttattcatat
     3361 ctgccattag caaatcgtgt attggaaaaa ctgaaacgca ttatgcggga agaatttgaa
     3421 aaaatcggag cggtagaaat gctgatgcct gccatcttgc cagcagaact ttgggaagaa
     3481 tccgggcgtt acgaaacata tggaccaaac ttgtatcgtt tcaaagaccg caatgatcga
     3541 aaaatgatcc ttggaccaac tcacgaagaa acttttactg atttgatgcg taatgaaatc
     3601 aattcatata agaaattacc tttaaatctt tatcaaatcc aacctaaata tcgtgacgaa
     3661 aaacgacctc gcttcggttt gcttcgcgga agagagttta tcatgaaaga tgcgtattct
     3721 ttccatgcaa cggaagaaag tttggacgag acatataaag attacgaaaa agcctacacc
     3781 gaagtattca agcgttgtgg cttagaattt cgctcgatta tcggagacgg tggcgcaatg
     3841 ggcgggaaag actcaaaaga attcatggcg atctcagaaa tcggggagga tacgatctgt
     3901 tattcgactg aaagtgatta tgctgcaaac ttggagatgg ctacaagcta ctatgtatca
     3961 aaaaaatcgc atgaaacaca gcttgaattg gagaaagtag ctacacctaa cgtaaaatca
     4021 attgacgaag tagcagattt cttttctgtt gaacctcaaa aaatcatcaa gtctgtattg
     4081 ttcattgcgg atgaacaacc tgtccttgtt ttggtacgcg gagaccatga agtcaatgac
     4141 gtaaaactga aaaacttttt atctgctgac tttttagaag aagcaacaga agaagaagca
     4201 caaaaatatc taggtgctga gtttggttct gtcggaccag tcggtgtttc agatgacgta
     4261 aaagtatatg cagaccgtta tgtccaagat ttagcgaatg ccattactgg agcgaacgaa
     4321 acaggattcc actataaaaa tgtgaatcca gaacgcgatt tcactgtact ttcttatgaa
     4381 gatcttcgtt ttgtacaaga aggagatcct tcaccagaca acaatggtgt tcttgccttt
     4441 actcgaggaa tcgaaatcgg acatatcttc aaaatcggca ctcgttacag tgaatctatg
     4501 ggagcaactg tactagacga aaatggacga gaaaaatttg tgattatggg ctgttatggt
     4561 atcggtgtaa gtcgtttatt atcagcaatc gtagaacaac attcagatga acaaggaatc
     4621 aactggccaa aaggaattgc gccatttgat ttgcatatcg tccaaatgaa tctaaaagac
     4681 gaataccaaa caaacctgac gaatgaacta gaagaagcga tgacaaaagc tggctatgaa
     4741 gtactggttg atgacagaaa tgaacgcgca ggagtgaaat tcgcggattc tgatttgatt
     4801 ggctgtccaa tcagaatcac tgtcggtaaa aaagcagtgg atggaatcgt agaagtgaaa
     4861 atcaagaaaa ctggcgaaat ggttgaagta cgtaaagaag aattggtgaa tactttacca
     4921 atcctcttga accaaggcga ataataagcg ttaaaacaca gaaaataaaa atagctgaat
     4981 cagctgtttt tgacaatctg atgattcgct tttaaaattg gaactgcgat ttgaatcgca
     5041 gttccaattt tttaatgtaa aactcagaaa aaacgagtat actaaaaggt actgaaaaag
     5101 aagaggataa gatgcgatca attgcattgt ttctcatcag aaagggcgta tatctttgtc
     5161 agaaaaacga gatttatttg aaaaactgtt ggaacaaatc caactagagg aagcagaaaa
     5221 aaatcatcca ctgcttactg ctggtgaaat ggatagtgta gtggtccatc gtaaatcgcg
     5281 tttatgggaa tttacgctgc atttttccaa aattttgcct attatgctat atcggtcatt
     5341 gactcagcat ctgactatcg cttttaaaga tattgcacaa gtcaaactga atatcttagc
     5401 agatgaccag acattcgatg aacagctctt gcaagattac tgggcccaag ctttggaaaa
     5461 tcagcaatgc gatacaccac tagcacagca agtattgaaa acgcaggtac ctgtgataaa
     5521 agatcgtaaa gtcatcttgc caatcgacag tacaggagct atttcttatt tgaagcaaca
     5581 atacttgcca ttagtagaag agttgttcgt cagttacgga ttcccaaaat ttcgtatcga
     5641 accagaagtg gatgagcagc aagcggaacg agtattgaaa ctttttgaag aacgcaaaca
     5701 agaacaggca gaagccttta tgaagcaagc agctgaaagt ctagttgtcc acgaacaaaa
     5761 gaaaaaggaa agaaaagagc aatcgccagc tcttgaagga cccatccaca tccagctagg
     5821 acgaaatatt ccagccgatg aaccaacgac accaatgatc agtatcgttg aagaagaacg
     5881 aagagtcaca cttgaggggt acgtattcga caaagaagtc agagaactac gttcaaaacg
     5941 caagatcttg actttgaaaa tcacagatta tacttcatca tttatcgtca aaaaattctc
     6001 gaacggcgaa aaagatgaac aagtatttga tgcgatcagt gtcggtagct ggttaaaggt
     6061 acgaggaagt attcaagaag atacgtttgt ccgtgatttg gtcatgaatg cgcaagatat
     6121 catagaagtc aaacatacac ctcgcaaaga ttatgctcct gaaggggaaa aacgagtaga
     6181 gcttcatgtt catagcaata tgagtacgat ggatgcaaca aacagcatct ctgatcttgt
     6241 ggcacaagca gggaaatggg gacaccgagc tatcgccatt acagaccacg gtggtgcgca
     6301 agcctttccg gaagcacata gtgctggaaa aaaagcaggt gtgaaaatct tatacggcgt
     6361 ggaagcgaat gtcgtagatg atggtgtccc aattgcttat aatgatgcac atgaagcatt
     6421 gagtgaagcc acatatgtcg tatttgacgt ggaaacgaca ggattgtctg cagtatatga
     6481 cacgatcatc gagctagccg cagtcaaaat gtataaagga aatgtgatcg aaagctttga
     6541 tgaatttatc gatccaggac atcctttgtc acgaacaacg gtagatctga ctggtatcac
     6601 ggatggaatg gttcgaggat caaagtcgga agaagaagta ttgcggatgt tccttgaatt
     6661 ttcaaaagat acaattttgg tagcccacaa cgcggctttt gatatggggt tcttgaatac
     6721 tagctacgca aggtatggta ttcctgaagc ggccaatcca gtcattgata cgttggaatt
     6781 agctagatat ctgtatcctc aattcaaacg tttcggatta ggtgtgttat ctaaaaaatt
     6841 tggtgtcagc ttggaacagc atcaccgagc aatctatgat gctgaagcaa ctggtcactt
     6901 agcatggatc tttgtgaaag aggcaatgga caatcataac atgctttatc atgatcagct
     6961 aaatgagcat atcggagaag gagattctta caaaagggct cgacctttcc atgtaacgat
     7021 tttagcaaaa aatcaggcag gactgaaaga tttgttcaaa ctgatttcaa tgtcgaatgt
     7081 cgaatacttc gaacgcgtcc cacgaatacc acgctcccaa ttgaaaaaga tgcgtgaaaa
     7141 tctattgatc ggctcagctt gtgataaggg agaaatattc gaagccatga tgcaaaaagg
     7201 ggtagaagag gcaagaaacc gagcaaaatt ctacgattat attgaagtta tgccaaaagc
     7261 ggtctatgcg cctttgatcg agcaagaatt ggtaaaaaat gaacatgatt tggaagaaat
     7321 tatccaaaat ttggtagaaa tcggaaaaag tttggataaa atcgttgttg caacaggtaa
     7381 tgtacattat ctgaacgaag aagatgccat ttaccgaaaa attttgatca attctatggg
     7441 cggagcgaat ccgttgaatc gtcatagttt gccggatgtc catttccgaa caaccgatga
     7501 gatgctaact gcatttcact ttttagggga agaaacagcg aaagagatcg tagtagaaaa
     7561 tacgaataaa atcgcagata tctgcgaaga agtgattcca gtcaaagatg aactttatac
     7621 accgaagatt ccaggttcag aagatgaaat atcagaattg agttacacaa aagcaaaaca
     7681 aatgtacgga gatccgttac ctgagatcat tcaaaaacga ttaaaaaaag agttgaattc
     7741 tattaatggt aatggcttct cggttattta tctgatcgcc caaaaactag tgcataaaag
     7801 taatgaagat ggctatctag taggttcacg tggctctgtt ggctctagct ttgttgcgac
     7861 gatgacagga atcactgaag ttaatccgtt agcccctcat tattattgtc cagagtgcca
     7921 atattccgag ttttttgaag atggtacgta tggttctgga tttgacatgc cagaaaaaca
     7981 atgtccaaaa tgtggtgcac gcttaaacaa agacggtcat gatattccat ttgagacatt
     8041 tttaggattc catggagaca aagtgcccga tattgatttg aacttctcag gtgattatca
     8101 agcagaagcc cataactata cgaaagtatt gtttggcgaa gactatgttt accgtgccgg
     8161 aacgattggt acagtcgccg acaaaacagc atatggctat gtaaaaggat acgaaaggga
     8221 caataatctg cagtttcgaa gtgcagaagt cgacagatta gcaaaaggcg ctactggagt
     8281 caaacggaca accggccagc atccaggggg gatcatcgtt attcccgatt atatggatgt
     8341 ctatgatttt acgccgatcc aatatccagc agacgatcaa aattcggaat ggaaaacgac
     8401 tcattttgat tttcactcga ttcacgataa tgtattgaaa ttagatattc tagggcatga
     8461 tgacccaact gttattcgga tgcttcaaga tttatcaggg atcgatccac aaacgatccc
     8521 tacggatgac ccggaagtga tgcggatatt tgccggtcct gaagtacttg gagtaagtca
     8581 ggaacaaatt tattccaaaa cagggacatt aggtattcca gaattcggca ctcgttttgt
     8641 tcgagggatg ctagaagaaa cgcatcctac aacttttgca gaactactgc aaatttccgg
     8701 actatctcat ggtacagacg tatggttagg aaatgcggaa gaattgattc gacgaggaga
     8761 tgcgacactg gcggaagtaa tcggctgtcg tgatgatatt atggtttatt tgatccatgc
     8821 tggattagat agcgggatgg catttaaaat catggaaact gttcgtaaag gacaatggaa
     8881 caagatccct gatgaattgc gagaaaccta tctatcggcg atgaaagaaa ataatgtgcc
     8941 cgactggtat atcgattctt gttcaaaaat caaatatatg tttccgaaag cccatgctgc
     9001 agcttacgta ttgatggctc ttcgtgtcgc ttatttcaag gtttatttcc ctattttgta
     9061 ttactgtgca tacttttctg tacgtgcaga cgactttgat ctagtctcca tgtgcaaagg
     9121 aaaagatgct gtgaaacaag caatgaaaga aatcacggac aaaggattag atgcttccgt
     9181 aaaagaaaag aatcaactga cagtgctgga gttagccaat gaaatgctgg aacgaggatt
     9241 caaatttgga atgattgatt tgtataaatc ggatgccgtt aactttgtta ttgaaggtga
     9301 tacgttgatc gcaccattta gagctgtccc aagtttggga accaacgtgg caaaacaaat
     9361 cgttgaagca agaaaagacg gtccattttt atccaaagaa gatcttgcca cccgcggtaa
     9421 agtttcaaaa acattgatcg aatatatgaa cgacaatggc gtattgaaag atctaccaga
     9481 tgaaaatcaa ttatcgttat tcgatatgct ataagcatct gttttcctgt ctgcttgtaa
     9541 ttttaagcag agtatggtat agttcttata ggtaatgata ctaactgtga gtgagcggat
     9601 ttttcgctca ctctttttaa tggaattacg cacttaattt tttaaggagg cgaaactttt
     9661 gagtagcgtc gttgaaacag tcacagaaat ggtgacaccg attttagatg aacaaaattt
     9721 tgaacttgtc gaagtcgaat ttgtaaaaga aggaaaaagc tggttcttac gagtatttat
     9781 tgacaaagaa ggcggtatcg atatcgaaga atgcgccttt gtcagtgaga aactgagtga
     9841 aaaacttgat gcagcagatc cagatccgat tcctcaagct tatttcctgg aggtatcctc
     9901 cccgggagca gagcggccat tgaaaaaaga aagcgactat gaaaaggcac ttggtgatta
     9961 tgtccatgtc tccttatacc aaccagtaga tggcgaaaaa caatatgaag gatttcttca
    10021 atcatttgat gctgaacagc ttacattgaa aatacgcata aaaacacgag aaaaagaatt
    10081 gacttttgac cgaaaaaata ttgcaaaagc tcgcttagct atccaatttt aatcacggag
    10141 aggaatcaga aaaaatgagt aaagaaatgt taaatgcgtt agacgcttta gaagctgaaa
    10201 aagggatctc aaaagaaatc gtgatcgatg ctttagaagc agcattggtt tctgcataca
    10261 aacgtcacta tggccaagca caaaacgtcg aagtggaatt tgatcagaaa aaaggaaaga
    10321 tccatgtcta tgcggtaaaa gaagtaacgg aagaagtgat ggattcacag ttggaagtct
    10381 cattgaaaga tgcactttta atcaatccag catatgagat cggtgatacg atccgttttg
    10441 aagtaacacc aaaagatttt gggcggatcg ctgcgcaaac ggctaaacaa gttattttac
    10501 agcgtgtacg tgaagcagaa agaacgatta tttataacga attcagtgct tatgaaaaag
    10561 acatcatgca aggaatcgtc gaacgccagg ataaacgtta tatttatgtc aatcttggta
    10621 aaattgaggc agttctttct aaacaagatc aaatgccaaa tgaattttat caaccacatg
    10681 atcgaatcaa agtttatgtt tcccgagtag aaaatacgtc taaaggtccg caagtatttg
    10741 ttagccgcag ccatccagac ctattgcgcc gcttatttga gcaagaagta ccagaagtct
    10801 atgatggaat cgttgaaatt gtcagcatcg cccgtgaagc tggcgatcgt tcaaaagtgg
    10861 cagttcgctc aaatgatcca aatatcgatg cagtcggtac atgtgtcggg ccaaagggac
    10921 aacgagttca agcaatcgtc aatgaattaa aaggcgaaaa catggatatc gtcgaatgga
    10981 atgaagatcc agctgtattc attgcaaatg cattgaatcc tgcacaagtc atggatgtga
    11041 tcttcgatga gacaaatcca aaggcctgca cagttgtcgt tccagactac caattatcgc
    11101 tagcaatcgg taaacgcgga caaaacgcac gtttagctgc taaattgact aaccataaaa
    11161 tcgatatcaa atcagaatct gacatgacgg aattttatga aaatcaagaa caacaaaaag
    11221 aaacagaaga actgcatgat gaagcaatcg ttcagtcaga tttaacagac gatgaatatg
    11281 aaacaattgc attcaacaat gaaacggtcg aagaaaaacc agaagcttaa ctttagtaag
    11341 gaggcaaaga gatgaaacaa aggaaaattc ctttacgcaa atccgtggtt tcaggtgaaa
    11401 tgaaacctaa aaaagagatg gtccgaatca cccgttcaaa agaaggggaa gtatcgatcg
    11461 atccaactgg aaaaatgcct ggacgaggag cgtatgtctc acttgagccg gaagaagtac
    11521 aacaagcttg ggataaaaaa attctcgatc gagtactcga aacaacatta acagatgagt
    11581 tttatcaaga acttcttgac tatgtcactc accaaaaagc acgtaaagag ttatttggaa
    11641 aatgaataca gttaatcggc aaaaagccat gaatctcatc ggtctagcga tgcgcgcggg
    11701 aaaactaatc acgggtgaag aactgaccat tgctgaaatc cgcaaacaaa aagcaaaatt
    11761 ggtgtttgtg gcaaccgatg caagcgagaa tacaagaaaa aagataaaag ataagagttc
    11821 atattatgaa gttccttgct ttgagctatt ttctgaagca gaaatcacgc agatgatcgg
    11881 aaaacctcgc aaggtattcg gaataatgga tgccggattt gcgaagaaga ccaaggagct
    11941 aattgaaggt taggaaggtg attgcatggg caacaaaaga atctatgaat tagcaaaaga
    12001 actcaaaaaa tctagtaaag acgtcgtgga aaaagcacaa caacttggca tcgatgtgaa
    12061 aaaccatatg ggtgcgatta ctagcaatga tgagaagaaa ttgcaacaag cgttcaaaaa
    12121 cccaagcacg aaacaacaag ctgcacaaca agcaagcaga aaaccagcca atcaacagcc
    12181 gaacgatcaa cgtaaaaaag aaacaacgaa caatcaacaa aaaaataaaa ataaccgcag
    12241 ttatcaagat cgcggtcaag ggagcggtca agtgaatcaa ggaaaaaaac caacaacaaa
    12301 ccaaaataat acgggaaata ccagcagcca aaatcgtcaa ggaacgagcc aaggcggcaa
    12361 tcaaaaccgt actaacaata acaacaaacc aaacaataac aatcgcggca aatttaataa
    12421 taataaccgt aatcgttata ataaaaaagg taaaaaaggc aaacagcaac aatcaactaa
    12481 accagctgta ccaccacgca aattccgtga attgccagat gtattagagt atactgaagg
    12541 tatgaacgta gcagatattg cgaaaaaaat tcatcgtgag ccagcagaaa tcattaaaaa
    12601 attatttatg cttggtgtca tggtcaatca aaaccaagca ctggataaag atacgattga
    12661 actgttagcg acggactatg gcatggaacc acaagaaaaa gtccaagttg acattgctga
    12721 tatcgataaa ttcttcgagg cagaagaagt cgatccagat aaattagtct cacgtccgcc
    12781 agtcgtaacg attatgggac acgtagacca tgggaaaaca actttattgg atacattacg
    12841 ccattcacgt gtaacaagcg gcgaagccgg cggaatcaca cagcatatcg gtgcctacca
    12901 gatcgatatt gatggtaaac caatcacttt cttggataca ccaggacatg cggcctttac
    12961 aagtatgcgt gcacgtggag caagtatcac agatatcacg atcttagttg ttgctgcaga
    13021 tgatggcgtc atgccacaaa cagttgaagc aatcaaccat gccaaagctg caggcgttcc
    13081 aatcatcgtc gctgtaaata aaattgataa accaggtgca aaccctcaac atgttatgca
    13141 agaattaagt gagtatgagt taattcctga agcatggggt ggagaaacga tctttgttga
    13201 gatctcagcg aaattcggcc aaaacatcga agaattgctg gaaatgatcc tgttagtcgc
    13261 agaagtagaa gatttaaaag cagatccaac acaacgcgca atcggaacag tgatcgaagc
    13321 acgtcttgat aaaggtaaag gtcctgtgtc aaccttactt gtccaagaag gaacacttca
    13381 tgttggtgac ccaatcgttg tcggtaatac ttacggacgt gtccgtgtaa tgacaaacga
    13441 tcttggccgt cgcgataaag aagcaggacc tgcaacgcca gtagaaatca ctggcttgaa
    13501 tgatgtacct caagcaggtg accgtttcgt agtcttcgaa gacgaaaaga cagcacgtgc
    13561 agcgggtgaa gaacgcgcga aacgtgcatt attagaaaac cgtgctgcca caagccgtgt
    13621 gactttggat aacttatttg aaagcctgaa agaaggcgaa ttgaaagaag ttaacgtaat
    13681 catcaaagca gatgtacaag gttcagcaga agcactggct gcatcattga agaagattga
    13741 tgtggaaggt gtccgtgtaa aaatcgtcca ttcagctgtc ggagcaatca atgaaagtga
    13801 tgtaacctta gcagcagcca gcaacgctat tatcatcggt ttcaacgttc gtcctacacc
    13861 gcaggcaaaa gtgcaagctg atactgaaga agtcgatatt cgtcttcacc gtatcatcta
    13921 taaagccatc gaagaaatcg aaactgcgat gaaagggatg ctagaccctg aatttgaaga
    13981 aaaaatcacc ggtcaaatga tcgttcgtga aaccttcaaa gtttctaaag ttggtacgat
    14041 tgcaggtgcc tatgtaacag aaggatatat tcgtagagat agcggtgtcc gtgtaatccg
    14101 tgacggaatc gtaatctatg aaggccaact tgcaagtctg aaacgattca aagatgacgt
    14161 gaaagaagtc aaaatgggtt atgaatgtgg tgcaatgatt gaaaaattca atgacatcaa
    14221 agtagatgac gtaattgaag gatttatcat ggaagaagtc aaaaatgact aaaaactgat
    14281 aaaaggaggc caatcattat ggctaactac cgtgaccgcc gagtcggtca agaaatctta
    14341 aaagaagtca atgatatttt gcgcaaaaag gtccgtgacc ctcgtgtgga gaacgtaacg
    14401 atcacagatg tgaaagttac gggggactta cagcaagcaa cgatctatta cagcatttta
    14461 tctgacttag catcagataa gaaaacagca caagatggat tgaataaagc atcaggattg
    14521 attcgtcgag agttagggca caatctaagt atttataaga cacctgaatt gttctttgaa
    14581 cttgatgaat cagttgtcta tggcaaccat atcgatgaat tgcttcgcga cttgaacaaa
    14641 gactaatcaa aaactcccgc aaatcctgtg aaatcagggg ttgcgggatt tttttgtttt
    14701 cattaagacg tattacctaa gagttccatg actttagtct tgcggcgtgt cagctaacca
    14761 aaaaagagaa aaagaaaagt aaaaaattcg actttctttt ttctagtagt atataaattt
    14821 ttgcatatag ataaagatca agataccgat aacggaagct agattgtaat ctattttagt
    14881 gacagtcagg tatagccaag taactagaag atcagtggaa cctgcaccat aaaagtttga
    14941 gtttgtcggt ccgttgacag ttaaaagaaa aatcacgttg aagttattga tatttccgat
    15001 tacgccgctt cgatttggtc acgatccaaa ttttggataa tccctgtaga aattagcata
    15061 gtagctggga taccaattca catattgaca aagataacag agacttttac ataaaacgga
    15121 tcaatcagga aaggaattgc tcgatccagc cccagttttc cagcaatgtg ttaactggcc
    15181 cagcgtcatt cagcaaatta tgcatgatga ggagcgaaat gaactgcgga acagccattg
    15241 tgataacgac aagttttttg gatcaatgta aaaaacgtca gcgaaaggaa aaatttcgtg
    15301 gttttttata cctgttcatt tggctagata taaaatccaa acaaatgaac agaagtgctt
    15361 attaggtttc aattgggagt atctgcgcta gaataagagt aataatgaaa aggagtgtta
    15421 tgtatggatt attctagtaa agaatacttt gataaaatga cggcttggtg gcgtgctgct
    15481 aattatcttt ctgttgggca actttatctt aaagataacc ccttactgag acgtacactg
    15541 aaacccgaag atgtcaaaaa acatccaatc ggtcattggg gaacgattcc tggccaaaat
    15601 tttatttatg tccatttgaa ccgagtcatc aataaatatg atttgaacat gttttatatt
    15661 gaagggccag gacacggcgg acaagtcatg gtatcgaatg cttatttaga tggaagttac
    15721 acagagatct atccagaagt aacggaagat gagacaggaa tgcaaaaatt attcaaacga
    15781 ttttcatttc ctggaggcat tgcttctcat gcagcaccag aaacaccagg ttctatccat
    15841 gaaggcggag agcttggata ctctctttca catgcagtcg gagctgtatt agacaatcct
    15901 gaagtaatct ctgctgtcgt gattggtgat ggtgaagcag aaaccggtcc tttagctgga
    15961 agctggttct caaatgtctt tatcaatcca gtgatagatg gagctgtttt accgatcctt
    16021 catttaaatg gtgcaaaaat agccaaccca acgatcttgg cacgtaaatc agatggagaa
    16081 ttagcaaatt actttaacgg acttggctgg gaacctttct ttattgaagg aaatgattca
    16141 gaaaaattaa atcctgttat ggctgaaaaa atggatcaag caatcgagaa aattaaatcc
    16201 atccaaaaag aagctcgcct gaagacagca acagatgtcg tgatgccaaa atggccagta
    16261 ttgatcgtac gcacaccaaa aggatggacc ggtccaaaag aatgggacgg ggaaccaatt
    16321 gaaggaacct tccgagcaca ccaagtaccg attcctgtag atcaagaaca tatggatcat
    16381 gcagatgctt tactccgttg gctgaaatcc tatgaaccag aaaaattatt tgatgcacaa
    16441 ggacgtattt tagaagaaat cagagaaatc gcgcctactg gtgaccaaag aatggcaaaa
    16501 aatcctatca ccaatggtgg aatcgatcca agaccgctta ttatgccaga ttggaaaaaa
    16561 tatactttgc aatttgaaaa accaggctcc gttaaagcag aagatatgac cgaacttggt
    16621 aaattcgttc gcgagatcat tgagaaaaat ccagaaaatt tccgtatatt tggaccagat
    16681 gaaacaaaat caaaccgatt gaatcaagtt ttcaaaacga caaatcgcca atggatggaa
    16741 aaaatcgaac cagaaaatga tgaatggctt tcgccatctg gtcgcgtcat cgacagtcaa
    16801 ttatcggaac accaagatga agggttctta gaagggtacg ttctgactgg acggcatggt
    16861 tttttgcaag ttacgaatca ttcttgcgag tggttgactc tatgttgact cagcacttca
    16921 aatggatgcg gaaatcacat gacctttctt ggcgaaatga ttatccttcg ctaaatctca
    16981 ttgcaagctc aacagtcttc cagcaagatc acaatggata tagccaccaa gatccaggaa
    17041 tattgacaca tttggcagaa aagaaggcgg agttcatccg tgaatatctg cctgcggatg
    17101 caaatacatt gctcgcagtc atggataaag cattccgctc gtcagaaaaa atcaatttga
    17161 tcatctcttc caaacatccg cgtgctcaat tttactcagc agaagaagca gccgtcttag
    17221 tgaatgaagg cttgaaaatc attgattggg cttcgacagc aaaagaggag gaacctgaat
    17281 tggtcattgc tgctgcagga acggaatcta acttagaagc attagcagct gtaactttgc
    17341 tgctggaaga attcccaaaa ctaaaaattc ggttcatcaa tgtcgtagat ttattgaaat
    17401 tacgtcatcc aagtcaagat ccgcgtgggt tgagcgatga agaatttgat caatatttta
    17461 caaaagacaa accgatctta tttgctttcc acggttatga aacattggtc cgcacgattt
    17521 tctttgatcg tcataaccat catttaatga ttcatggcta caaagaaaat ggagatatca
    17581 cgacaccatt tgacatgcgt gtagtaaatg aacttgatcg ttatcatttg gcaaaagacg
    17641 cagctttaaa aatcaaagga agtcaagcag aagattttgc gaaaaaaatg gatcagaaat
    17701 tacaagaaca tcaaaactat attcgtgaaa atggtattga tctgccagaa gtattggatt
    17761 ggaaatggaa aaatcttgac caataaaatg ataaaatcga tcccgcaaaa tagaattttt
    17821 tacattgatt aagaaactga aaaaataaat aaaaaaaacg gaaaaaagac tggaagaatg
    17881 gccagtcttt tttttcgttt ttgggaagtg aaaaccaaac tatttacctg taagccaccc
    17941 ataaatccaa tcaaatggtg caaagaaaaa atcttccatt ctattccctc ctttaaaaaa
    18001 gcataacatg atttctattt acgtgagcat gaattttgtt acgtttttat tgccgaaaaa
    18061 taaaaaccgt tcattatgat gtcaaattcg tcagtaattg atgcaatgtg ttataataat
    18121 tcagttaaat caaaaatgaa atggagaaaa gacatggacg ggttattacc tttatggaaa
    18181 gaacgcggaa tgacaagtca tgattgcgta ttcaaattaa gaaaaatctt acatacgaaa
    18241 aaaatcggac atgggggtac gcttgaccca gacgtagacg gtgtgttgcc catttgtatt
    18301 ggaaaagcca caaaagttat cgaatatttg acagattctg ggaaaatcta tgtaggcgag
    18361 ataactctag gtttcagtac aacgacggaa gatcgatccg gagaacgggt agaaacaaaa
    18421 ccagtagata tgtctttatc tgaaaaaaaa tagatgaagt gatggcttca tttattggtg
    18481 agatcaccca gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg
    18541 aatatgcccg caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt
    18601 ttatccggac aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg
    18661 aagtagaatg cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac
    18721 ttggttaccc tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga
    18781 aggatgctct gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt
    18841 gtttgctgcc aatagaaaaa ggagtggaac ggttcaaacg agtagatatt actgatgaag
    18901 tatggcagaa agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa
    18961 tgccgtcaga agaaattgct ttgttttata aaggaaaagt tgtcagtatt tatcagccaa
    19021 acccgaaaga aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa
    19081 tgaatatcat caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg
    19141 ttttagtgct gggatttttc gacggcgtgc atatcggaca ccagaaagtc ataaagacag
    19201 gtatggaaat tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc
    19261 catctattgt ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc
    19321 aaaaagaaga aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt
    19381 cttcttttgc tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg
    19441 caaaatatgc tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg
    19501 ctcattttcc tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag
    19561 aacaaggtga aaaaatcagc tctacccgta ttcgtggatt actagacagc ggacaagtaa
    19621 aagaagcagc aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg
    19681 atgcacgagg cagactacta ggattcccta cagcaaatgt caaagtaaag agcacggttc
    19741 atttgcctaa agaaggtgtt tatgtgtcgg aaataaaaat tggggaaaca tggtatccag
    19801 ccatgggttc aattggacat aatgatacat tcggtgaagg acgggagctg actgtagagt
    19861 tgtatatttt ggactttaat caagatatct atggtgaaca tgtcgcggta cgctggaatc
    19921 aatttttaag agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaagg
    19981 aggatgaaca agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct
    20041 cttgtttatg tataactagc aattacaata aaatgggtga taaataagcc caggaaaggg
    20101 gctgtgacaa aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc
    20161 ttcttcggaa ataagccgaa atttcttgaa gctgaccact tctgtcacaa cctctttctt
    20221 tatagaaata aaaggacact agcaattcat gaaaacgatt gctagagcgt atcatttgct
    20281 ttagactata tatcttggtg tacactcact ttaaggcgga aataaaagtt atccgtaata
    20341 acaggtttag gtatgaaaaa ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg
    20401 cagtagtaac tggtggctca aaggggattg gaacagcgat tgctcaacga ttcgggaaag
    20461 aaaaaatgaa tgtagttgtc aactataact cggataaaga aggagcagaa aaagcagtca
    20521 aagcaatcga agaggcaggc ggaaaagcag tctctgtaca agcagatgtc agttctgaag
    20581 aaggaataca gcgtttactt gatacggctt tagaaaattt tgggacactg gacgtttggg
    20641 ttaataatgc tgggatggaa aatcagcatc caacccatga acttagcctt gaagattggg
    20701 aaagagtgtt aaaagtcaat ttgactggcg tttttatcgg aacgaaagcg gctttaaatt
    20761 actttacaga gaataataaa aaaggaaata tcatcaatat gtcttctgtt catgaacaga
    20821 tcccatggcc aacgtttgct cattacgctg cttcaaaagg cggggtaaaa ttatttaccg
    20881 aaacggtagc aatggagtat gcaccgaagg gaatcagaat caatagtata ggccctggtg
    20941 ctatccgtac acctattaat gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg
    21001 aaagtatgat cccactagga cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat
    21061 ggttggcttc agacgaatcc agttatgtta caggtattac actatttgca gatggtggga
    21121 tgactttata tccttctttc caaggtggaa aaggataagg gtgaataaaa atgggtttat
    21181 tgattgcatt gatcccggca gttggctggg ggatccagcc actgattttg aaaaagattg
    21241 gtggcagacc aacaaatgag atattaggaa ctggtattgg agcggtgctt gtcggcttac
    21301 tcgttcaatt attcgcttct ccagctggta tttcagtgac tacatttttg attagtttac
    21361 tttctggtgc attttgggtt attggacaaa caggacagta tacatctttc aacttgattg
    21421 gtgtttctaa aacgatgcca atttcgactg cattacagtt agtcggaacc tcgttgattg
    21481 gtgtcttcgc ttttggagaa tggtctggaa gtatggggaa aatcattgga gcagcagcaa
    21541 tcattcttct tgtatttggt tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag
    21601 gcggtatcac aaaaggaatt agtctattag catcaacaag tattggttat tgggtataca
    21661 gtgcattgcc taaactagtt gatgcaagcg gactttccat tttctttccg caaatgatcg
    21721 gtgtcttttt aggggcagtg atttatgtga ttttccgtaa accaaccgca ttttctgaag
    21781 acaagagttg gaaagcaacg atcgttggag ccatctttag tgtgtcagca ttggcttata
    21841 ttttttcagc aaatgaaaat ggggttgcta ctgcatatat cattacacag ctaaacgtag
    21901 ttatctctac attaggcggt atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa
    21961 aattaacgat tatcggatta ttgctgatcg tagtaggaag catggtcacg gtctttattt
    22021 gaggataaag atggtaaaat ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa
    22081 gtacttataa aaaaatagcg ctagaatttc caacatctct ggaatttcta gcgcttttta
    22141 attgtttaag atacaaggga aatatctgtc tggatcaata atttttcatt gatccattga
    22201 ctaaagttta cgacataatt gaagttacca ccgattagta tctccgcatg attgtcttta
    22261 taaagatcgt attgtaaaag acggtctttt ttatttgcgt aaacatcgtt atggtactgg
    22321 actaattgtt ctcggaattg attgattgct gtaaaatcac gatattgaag gttgaaacgc
    22381 atcgtgattg gtgcatttag catgaatcgc tgcatttctt ttgctgcttc ctctgcttga
    22441 tctccgtata ggaactcaaa aagattacgt tcagaaaatt tccattgccc gccaaccttt
    22501 gttcctttta gttttccttc atgaagataa tttctgatag aacgtgtagt aacacctaat
    22561 tgttcggcta attcatttac tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg
    22621 gttctactga cttagttgat atttgcttaa tcacgaaatg tagaaagaac gattttaatt
    22681 aaggtcattg taaaaatcaa gtcagaaaat tctaactttt atttctctaa agaaattcgt
    22741 tctctcctaa agtgatatta tatttaaata tacctgaaaa taatgggcag tattatgaaa
    22801 gatcactgat attttctcgt attttttcct tattcaaaag ttttgcagga aaattgctga
    22861 aaaaataatt ttactatgta gaaaattcaa agccattcaa tcagtgattt ttttcagcac
    22921 aagaaagact ccttataaaa atagcagata ctttgtcaat taagtgttta accagggatg
    22981 ataaaaagga taggattgaa caaaacctgt ataaggtgat gaatagtttc ctcgccagta
    23041 aaaggtttat tcgtataaag ctttatcgcc aaaatttatc atgacctttt ttatcttcaa
    23101 tattaaatga aatcaattgt tcaatcaatt cccagttgat tacacttttc caaggaactt
    23161 tgatgacgtt atccgtatat acgtagcctg cttcttggat atttttttca agggcacgaa
    23221 tcgctgcttt ttccggtgcg atagaaaaat gatttttagc aaaactaaat gcaataataa
    23281 aggtaccatg gtcagtaaac ataggttggt tccattttac agttgtgtct agctggggat
    23341 acgtttctgt tatcttagca aaaaggtctt tgatcttttg ttgatgttct ttttctttga
    23401 tttgattgat gatcggctga aatttttcca ttctaaaacg cctccattaa gtggtttgat
    23461 ttattttacc agaaaaacaa tagttatgta taaaaaacga acaaaacaca agtaatgttg
    23521 taaccgctat atttatatga taagatgtct caaataataa aatagaaaag agacataaaa
    23581 atgattgact tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat
    23641 cgagccattt atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt
    23701 acagagacca aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact
    23761 tttactacga aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct
    23821 attgtttcat cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg
    23881 tttggttatg aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa
    23941 cttgtttttg caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac
    24001 aaaatcatcc agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat
    24061 taaccaagta aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta
    24121 gaaagaaatg caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat
    24181 tcgttactcg aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga
    24241 aagacgcttt ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa
    24301 ttcaatcaaa tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa
    24361 ttattgattg agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta
    24421 ggttattcag atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact
    24481 gcacatggaa ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg
    24541 tgggaaagtg tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag
    24601 tatcaaaaag tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca
    24661 acaaattggc aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt
    24721 tgtattgata cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa
    24781 agaaagtata atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca
    24841 gttgatttaa gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg
    24901 gggatcatgt tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta
    24961 tacgaagatc tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat
    25021 gtaccgccac aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga
    25081 aaagcgactg ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa
    25141 agaccaataa atctaaatag aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc
    25201 tttttgtata acatattaga aagtctaatt atctataact gtcacatcga ttaactgttt
    25261 cacgtcattg ggatttcctg taggaatagc tactacagat gcattatcaa tgatttcttg
    25321 agttgtttct ctaatgaacg ctggataaga tgtttgatct gcaggcattt gccaatgtga
    25381 aggaactgga taactagtga ttgtcccgtc atggttactt gaatacacta aaactccctg
    25441 gtaactgtat ttcccagtga gcataaccac atctttcgga tagactgcac tccctgcatc
    25501 ataaggggcg ataggtgttc ctgcaggata atgaataaca tgtaattcga aatcacccga
    25561 tgtatctcta aaagtattac ctctaatagc tagccaaaca cgtgcgtact caatttgtag
    25621 atcagaatat cctgctaacg gatctattga ctgagaagac tgtgaggagg ttgtgtctat
    25681 tgttgaagaa gaaatatttg tctcaactgt actactagat gtagaagaag tattgggctc
    25741 tgagacttta ctgcttgttg aagatgtatt cgtttcagta gaagctgatt tctgagtact
    25801 tgatttttca gctgaactgg tttgtctgat tgaacttttg tctgtggtcg cagatggatc
    25861 actattttct ttactaggag acgtacatcc tacaaggaat aaagagacca acaataaaat
    25921 aaaacctaac tttttcatat aaaatacctc attatttttt taataccagt atagcataat
    25981 cctgaatttt tgaatccgta aaaccttgtc tatgaatata aatagagtgt atcttctttg
    26041 ataaatgtca gaaaaagtgt gacaaataat catcagtaaa attacttgtc acactattga
    26101 taaggagtat ttaattgaag agaatgatta ggagaaagga tctccgtact ttttatagta
    26161 atcatagaat ccaagtttgt atagacgaat cgttttttct cttatttctt ctgtttcaaa
    26221 atggactgct tgaagaatca agtctgtgaa ttctatagga gctgttccat tcgcagtaat
    26281 caaattacga tctttcacag cttgttgttc tataaaatct tctttattct gataattttc
    26341 cgattctaac caaggaaatt gcgcatttcc ggtgtgtttg taaccttgca ataatccgct
    26401 ctgagctaaa taatcaactg ccccacaaat agcagctact ggtacgtctg tttttaacaa
    26461 tttatcgatt atttgatata aatgatgatt ttttatgtcc catgaattgc caccgatcat
    26521 aataaaaagg tcaacttttt caggtatttg ttcgattaag taatcgattt ttgtttttaa
    26581 tcctccaatg gaagtgacct ctgattggat agaagcagtt ttgttttgcc atttctcatt
    26641 ttggtttagt gtgctagtaa tataagcacc ttcccaatcg gcatattcct ctaatagaaa
    26701 cgtaatcgca gttttcattt tcaagctctc ctttactatc tataacaagg acaacagttt
    26761 taacctacag acattcatta aaattgggta gcgacagcga aaacaacaat tcttaagttt
    26821 tagacagagt atacagatag tcaacttaat taaatacgaa ctaaataaaa atgacaggta
    26881 atcatttttg tttaccagca ttataacatt ttgcatacat ggatgacaaa agaaccaatt
    26941 cccacttgct tgagtacaat attaaaagaa actggaaggc aaaaagtaat tatagaaacg
    27001 aaaaataaat catatcgggc actcagacat gaaaacaacc ctagagatat ataactaggt
    27061 aaccagttta atgaaagaaa aaatcatcca agaagtcgat tcttggattt tttagtggat
    27121 ttgttcgcca aatacgttta gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc
    27181 tgagcactta aggaggaatc tgtgaaaata gcttttcata tttttgcaga ttttgactta
    27241 atgccgaagg tcagctttgc gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc
    27301 tccgatgaca ataagcaagc atttttagag ctaggtttcc ttgattctaa atatgcggct
    27361 tatttcaatg agttggtgct tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt
    27421 tcgacgtaag gagaaagaat ttcaatgata gaaacaataa atcccgcaga aaacaaggtt
    27481 tctgcgtaca ttcatatccc attttgcgag catatctgct attactgtga ttttaataaa
    27541 gtttttttag aagggcagcc agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa
    27601 ataatgctag aaagtcatcc gacagcgaat ttagaaacac tttacgtagg gggcgggaca
    27661 ccaacttctt tatctgctaa acaacttgat cgactgttat caggtgctag agagatcctg
    27721 ccttttcaaa atggtaatga gtttactgta gaagcgaatc caggtgattt gacccgtgaa
    27781 aaacttcaag taatgaaaaa ctatggcgtc aatcgtttat ccatgggagt gcagacattt
    27841 gataatcgct tattgaaaaa aattggacgt aaacacaccg cagaagatgt gtatcaaact
    27901 atgcaatttt tagaagcaga aaattttacc aatgtaagca tcgatttgat ttatgcatta
    27961 ccgggacaaa cactagaagg atatctggat actttggaaa aagctttggc tttggatctg
    28021 cctcactatt ctttgtactc attgatcctc gaaaacaaaa caatgttcat gaactgggta
    28081 cgacaaggaa gactcgaact gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg
    28141 attgaagcaa tggaaaagca tggacgtcac caatatgaaa taagtaactt tgggctctcc
    28201 ggacatgaat ctcaacataa cctcatgtac tggaacaatg accactattt tgggtttgga
    28261 gctggtgcta gcggttatct tggagatatt cgttaccgca acaaaggacc aatccaacac
    28321 tatttacgcc cattacgagc aaatgaacgc ccggtattgg aagaagaaaa attgacacga
    28381 aaaaatcaaa tagaagaaga aatgttctta ggactaagaa aaaaaacagg gattactaaa
    28441 aaacatttct atgatcgcta tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg
    28501 gaattagagc aagaagggtt acttgttaat gaagaagacc gtgtttattt gacaccaaga
    28561 gggacctttt tggggaacaa tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa
    28621 tatttgtttt ttatctgaat ttaaataaaa aaagttcaaa aatacaataa aggactattg
    28681 aaacgttgat ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag
    28741 tgctaaaaaa tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg
    28801 gattagcact tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac
    28861 aaaacgacat tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt
    28921 ccaaaacatt gatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga
    28981 aagcattaga agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat
    29041 ctgctttagg ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag
    29101 atgatttgct gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta
    29161 ttaagcaatc tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac
    29221 ctgaaataaa agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga
    29281 ttattgctat cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg
    29341 aatatatggg aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg
    29401 gagatcctct gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata
    29461 aatatttcca aacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt
    29521 ttgaagataa ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag
    29581 atcttgatca gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa
    29641 tgatcactcc gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt
    29701 tattgcagaa tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta
    29761 caatagcctt gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg
    29821 tttatcgaag agaactagca gctaaacttg ctgattacta ccgttcgctt gacttgtcaa
    29881 actcgtaatg tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag
    29941 caatcaaaca gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag
    30001 aactagataa agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag
    30061 aagattcggg tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg
    30121 agttagaaga aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa
    30181 atcgaggaaa aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa
    30241 aattacttcc atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc
    30301 aaggggcagg tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg
    30361 aagaagaagg aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc
    30421 aagcagtaca aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac
    30481 tacaagaagg ctacaaatta catgatcgcg tgttaagacc aaccatggtc attgttgcac
    30541 aataattgtg cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag
    30601 taaaattatc ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa
    30661 cgaagcaaaa atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt
    30721 caaaaatgga gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa
    30781 tacgatttca tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg
    30841 taaatcatat acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc
    30901 agaagactat ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa
    30961 tgatgcacaa cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg
    31021 tatcgtcaat gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga
    31081 cgaaaaaatc ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact
    31141 aggagacggt gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga
    31201 ctttgataac aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga
    31261 cttgtctcaa gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa
    31321 agatttatct ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc
    31381 tggaccattg catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga
    31441 cttagtagaa cgtacaaaag taccagtacg tcaagcgcta aaagatgcag gtttatcaca
    31501 atcagaaatc gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga
    31561 agcagtacgt aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt
    31621 tgctatgggt gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt
    31681 acttgacgtt acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt
    31741 gattgaccgc aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga
    31801 taatcaacca gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa
    31861 caaaactcta ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca
    31921 aatcgaagta acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct
    31981 aggtacacaa aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga
    32041 aatcgaacgt atggtaaaag atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga
    32101 ggaagttgac ctacgtaatg atgtagatgc attgctattc tctgtggaca aaacattgaa
    32161 agaattagaa ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga
    32221 attgaaagcc gctgttgaag caaacaacat cgaagaaatg aaaactaaac gcgatgcatt
    32281 gaacgaaatc gttcaaaact tgactgttaa actatacgaa caagcggcac aacaacaagc
    32341 tcaagaaaac ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga
    32401 agaagtcgat ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg
    32461 ctactttggc tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa
    32521 tatgatatga taacaagcga cgtgaaaatg atgagaacca ataggaggaa agcctatggc
    32581 aacaaaacgt gattattatg aagtcttagg gctctcaaaa ggagcatcag aagatgaaat
    32641 aaagaaagct tatcgtaagc tttcgaaaaa ataccatcct gacatcaata aagaacctga
    32701 tgcggaagaa aaattcaaag aagtatcaga agcatatgag attttaagtg acccgcaaaa
    32761 acgcgctgca tatgaccaat acgggcatgc aggcacagat ccaaattacg gtgctggtgg
    32821 agctggtggc ggatttggtg gattcggcgg cttctctggt ggcggcttcg gtggttttga
    32881 agatatcttc gactctttct ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg
    32941 tcaaggtgct gatttgcaat atacggtcga cttgtctttt gaagaagcga tttttggtgt
    33001 agaaaaagaa atcaagtata atcgggaaga aatctgtcat acttgtggtg ggaacggtgc
    33061 aaaaccaggc acacaaccaa ctacttgtca taaatgtcat ggtgcaggta cgattaatgt
    33121 agaacgccaa acaccacttg gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg
    33181 gacagggaaa gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac atgagaagaa
    33241 agcacattca gttaaagtga atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt
    33301 agctggtcaa ggagaagctg gagaaaacgg cggaccattt ggtgatttgt atgtggtctt
    33361 ccgtgtagaa gaaagtgata tctttgatcg tgaaggttct gaaatttact atgaattgcc
    33421 tttaagtttt gtacaagcag ctttggggga tgaagtccaa gtaccaactg tccatggaga
    33481 tgtgaaattg aagatccctg ctggaacaca gacaggtacg aacttccgtt tacgtggaaa
    33541 aggcgctccg aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag taaaactgat
    33601 cacaccgaaa aatctaaatg aacaacaaag agaagcatta cgtgcattcg ctgaagctgg
    33661 cggcatcaaa gttgaagaac agcaagaaga cggtttcttc gataagttta aagatgcttt
    33721 tggcgggaaa aagcggaagt agtttttcta gtaaaaacgc taagtcatta ataattaaaa
    33781 accagacgaa caatcatatg ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca
    33841 aaaaagccag ctttagaata tgataatcgt catagtttct aggggtataa ccagtgagag
    33901 aatggaactt gttcaatcga tattgcaagg tgtttttatg aataaataat tcttctgagc
    33961 aatttttgat gcttccgtta tttttactga agcatgccaa agtcttcttg aacaaatgta
    34021 cttcttcttc tgtcaggctt ttcaaaatac gttttacata taatgttcgt tgatctgacg
    34081 ggattgctgg taaaacgatc ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag
    34141 attgatggaa agctatccat tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct
    34201 cttgctctgt ataacaaagc gacccaattc caaagtttag ctgtaactga tacattttat
    34261 taaactttgt ctggatttta tcaagcaatg atagcaatga ggctctggaa atccgattga
    34321 ctaggataat gatttgttgt ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga
    34381 ccctttcgag caactccaat aaaggattga tccgttcttg acacaattgc tgattgtcag
    34441 aaagttgtcc cacaactaca taatatggag aagaaaaatc aagctcaatt aagtgagggt
    34501 ggtatcgact atgtacttga tatcatcaat gaaccgatgg tactttctta tgcgatggaa
    34561 caggtagagc gattgcttgt ttgtgcagga aaacaagcga tgcggctgat acgattaggt
    34621 gagaaaataa gaagataaaa atacacggtc ccttcatggc gaccgtgcat ttttggtttt
    34681 tattaaatat ttaatacttt attcaagaaa tcttttgtcc gttcattttg cggattttca
    34741 aagatttgtt gaggcgttcc atcttctaaa aagtttccgt cgtcaatgaa catgacgcga
    34801 ttggccactt ctttagcgaa ccccatttcg tgcgtaacga taaccatcgt cataccttgt
    34861 tctgctaact ttttcatgac gttcaagaca tcaccgacca tttcaggatc gagtgcagaa
    34921 gtaggctcat caaaaagcat gatgtcaggg ttcatcgcta gagcacgagc aatagccaca
    34981 cgctgttttt gtccaccaga aagcatttca ggatacatat cttttttatc ttctagacca
    35041 actgtctgca gtaatttgat tgcttttttc tctgcttctt ctctagattc tttcttcagg
    35101 tccatcggtg ctaaaatgat gttttccaaa atagaaagat gagggaatag gttaaagtgt
    35161 tggaatacca ttccgatatg ttgtctcacc aagttgatat ctgtgctttt atccatcaag
    35221 tgggcgccgt caataataat ttcaccgtca gaaggttctt ctaaacgatt caagcaacgc
    35281 aagaatgtac tttttcctga acctgaaggg ccaatgatac agacgacatc tccttcgttg
    35341 atggatacat tgatgtcgtt taatactgtg ttatcgccgt attttttgac taaatgctca
    35401 actaagattt tttctgccat cttacttcac cttcttttct aaaacttttg ctaattttgt
    35461 caatgctgtg atcaaaatca agtacatgat cgcaatgatc aagtaaacat acgtactttg
    35521 tgttgtacgt gcaacaatga tcttaccagt ttgcagcaat tcgactacac cgattgcgga
    35581 aatgatcgtt gtgtctttta atgagataac gaactgattg acgaatgatg ggatcatgat
    35641 gcgtatcgct tgcggcaaaa tgattttttg cattgtccga ttataagaaa gacctaaact
    35701 tctggaagct tccatttgtc cgacaggaac ggcgttgatc ccgcctcgaa caatctctgc
    35761 gatataagca cttgcgttaa gagtcaaggt gataacacca gcaaggaagt caggaatcgt
    35821 aaatccaacg ataccaggca acccaaagaa aataaagaac gcaagaacca tcattggaat
    35881 tcctcggatg atatcgacat aaatactagc aattgtgcga agagcgcgta ttggtgctac
    35941 actgaataat ccaaaaatca caccgattac tatagccaaa gcaaaggaga tcaaagttag
    36001 gagaattgtt ctccaaagtc ctttcaacaa tgcttgatag ttgttttgga taagtccaag
    36061 aaatgttgat tcatcagcag tggaagactc actggaagtt tctgaaccat ccgctacata
    36121 ttctttaata atcttatcgt attcgcctgt tctttttaat tctttcaatc cttcattgaa
    36181 catattaaga agctcaggat tttgtccttt tttaacggca aatgcatatt ttccgccaga
    36241 ttcttttttg atcggtgttt ccaattcttg tccttgtttg atcgcataac cgattaccgg
    36301 ataatcatcc atcattgcat cgattgcgcc aacttttaac ccgtcgtaca attgatcagc
    36361 agcatcaaaa tatttgatag aatagccata ttcagattca tggtcgctta aaaattcagc
    36421 actttcggta ccaacttttg ctccgactgt ttttcctttc aaatcttcat aagacttgat
    36481 cgaagtatct ccttttttga cagctaattg gatcccgcta tcaaagtaag aatcagaaaa
    36541 atcatagctt ttctttcgtt catctgttac agtcattccg gcaatcattc cgtctgcttg
    36601 tcctgattcc aaagcttgca aagcagcaga gaaaccacgg tagtccattc ggatgttaaa
    36661 tccttgcagt ttggcgatgt tttccatcaa ttttacatcg atgccttcgt actcgccctt
    36721 tgcgttttga aattcaaatg gagcaaaagc agagtcactg gcaattacgt atacgtcttt
    36781 tttgggctct attttttgca ttgtctcgct tgtgcttgac tctgccgttg tagtgttgtc
    36841 agcttgtgca acatgaccaa acgcaaatgt cacaagtcct gtcattaaga aaacaaatga
    36901 gtgaaataaa tgctttcttt tcataatcaa cctcctaaat ttccaatatt ttattattat
    36961 aggcgaaaag tgctgaatca gacaagactg taagctcgaa aaaatgaaaa aaatgcgagt
    37021 ttctaacatt ttatgttata aaacataaca tgtataataa gataaaaaag tggcgaaagt
    37081 atgttcggtg tggtataatt ttttagtaag gaatgccttt gctttccttt tctttttatt
    37141 ggtcgtaaaa tgaaaaggaa tgaatgaaaa ggaggacgaa gaatgattga gagagatacc
    37201 tcccgacact ttgagcttgt ctcgaaatac caaccggctg gtgatcagcc tgaagcgatt
    37261 aaccaattag tggatggcgt agtcggcggg aaaaaagcac aaatcttatt aggtgcgact
    37321 ggaacaggga aaacatatac gatttccaat cttattgaga aagtgaataa accaacactg
    37381 attattgccc ataacaaaac attggctggt cagctgtacg gggaattcaa ggaatttttc
    37441 cctaacaatg ctgttgaata ttttgtttct tattatgatt attatcagcc tgaagcttat
    37501 gtgccttcaa gcgatacgta tatcgaaaaa gattcaagtg taaatgatga gatcgacaaa
    37561 ttacgacatt cggctactag ttcattatta gaaagaaatg atgtgatcgt cattgcatcc
    37621 gtttcatgta tttttggatt aggctcgcct tttgagtacc aaaaacaagt agtttcgatt
    37681 cgacagggag cagaactaga tcgcaatcag ttgattcgtg acttagtcag tatccaattc
    37741 gaaagaaatg atatagattt tcagcgcggt cgttttcgag tgcgcggtga tgtcgtggag
    37801 atttttccgg cttcaagaga tgaacgtgcc ttacgtgtgg agttttttgg agatgaggtc
    37861 gaacgtatcc gagaagtcaa tgcactgaca ggtgaagttt taggagaaac ggaacacgta
    37921 gcaatcttcc cggcgactca ctttgtgacg aatgatgaac atatggagca tgccgtagcg
    37981 aatatcaaag cggaattaga acagcgttta actgtcttgc gaaatgaaaa caaacttctg
    38041 gaagctcaac gtttagaaca gcgaaccaat tacgatattg aaatgatgtt agaaatgggc
    38101 tacacgtcgg gaattgaaaa ttattctcgg catatggacg gacgtaaaga aggtgaaccg
    38161 ccttatacgc tattggattt ctttcctgaa gattttttga tcgtggcgga cgaatcgcac
    38221 gtaaccatgc ctcaaatcag aggaatgtat aatggtgacc gagcgagaaa acagatgcta
    38281 gtagattacg gtttccgttt gccatctgct ttagacaatc ggccacttcg gttagaagag
    38341 tttgaaaaac atgtcaatca gatcatttat gtctctgcga cgccaggacc atatgaacat
    38401 gaacaaacag ataccgttat ccaacaaatc atccgtccga cagggctgct tgacccagtg
    38461 atcgaagttc gtccaatcat gggacaaatc gatgatctag taggcgaaat aaatgaacga
    38521 gtcgaaaaag accagcgggt gtttgtaacg acattgacga aaaaaatggc agaagacctc
    38581 acagattatt tcaaagaatt agggatcaaa gtcaaatatt tgcatagtga tatcaaaacg
    38641 ctggaacgaa cagaaatcat tcgagatctt cgattaggtg aatttgatgt gttagttgga
    38701 atcaatttgc ttcgtgaagg attagacgta cctgaagttt cccttgttgc tatcttggat
    38761 gcggataaag aaggcttttt acgtagtgaa cgttccttag tccaaacgat tggacgagct
    38821 gctcggaatg aagaaggtaa agtaatcatg tacgcagaca aagtaacaga ttctatgcgt
    38881 ctagcgatgg atgaaacgtc aagacgtcgt acgattcagc agaagtacaa tgaagaacat
    38941 gggatcgttc caaaaacgat cattaaagaa atccgagatt tgatttctat tacgaaagaa
    39001 tcggaagatg atacaaaaga agcggtacaa gtctcgtacg aagaaatgac gaaggaagaa
    39061 aaagacacgt tattgatgaa attagaaaaa gaaatgaaag atgcggcaaa agctttggat
    39121 tttgaaacag ccgcgaatgt gcgagatatg attttagaat taaaagcttc aaagtaggag
    39181 gacaacatgg ctaacgataa aattacgatt catggtgcac gtgcccataa cttgaaaaat
    39241 atagatgtaa cgattccacg agataaattc gtggtcgtta caggtttgtc cggttctgga
    39301 aaaagttccc tagcatttga tacgctgtac gctgaagggc agcggagata cgtagagagc
    39361 ctttctgctt atgctcgtca gtttttagga caaatggata agccggacgt agatagcatc
    39421 gacggattga gtccggcgat ttctattgat cagaaaacaa caagtaaaaa tcctcggtcg
    39481 acagtaggta ctgtgacgga aatcaatgac tatttacgct tgttgtttgc acgtgtagga
    39541 catccgattt gtcccaacga tcatatcgag attaccagtc aatcagtaga tcaaatggca
    39601 gatcaagtaa tggctttgcc tgagcgcaca aagatccaag tgttggcacc gatcgtggta
    39661 aagaaaaaag gacaacataa aaaaatattc gaacgaatcc aaaaagaggg atacgtccgc
    39721 gttcgagtcg atggtgagat ctatgattta agtgaagtgc ctgagctgga gaaaaacaaa
    39781 aaacatgata tcgcgatcgt catcgaccgg atcattgtga aagaagggat tcgttctcgc
    39841 ctatttgatt ctttagaggc ggccctgcga ttagcagacg gctatgctct ggttgacgtg
    39901 atcggtgaag aagaaatgtt gtttagcgaa cactatgcct gtccttattg cggatttact
    39961 gttggcgaac tagaaccaag actgttctca ttcaatgcgc cttttggagc ttgtccagac
    40021 tgtgatggtt taggtgtcaa attagaagtc gatcaagatt tagtgattcc agatagaacg
    40081 aagacactta gtcaaggtgc tatcgttcca tggaatccaa tcagctctca gtattatcct
    40141 caaatgttag cgcaggcttg tgaaagtttc ggtatcgatt tagatacacc attcgaagaa
    40201 ttaccggaag agcatcagga gatcatcttg aatgggacag atgatctttt ccatttccat
    40261 tatgaaaatg attttggtgg cgttcgcgat gtagaaacga ctttcgaagg ggtattgaag
    40321 aatatcgagc gtcgttatca tgatacgaac agtgatttca cgcgtgagca aatgcgtctt
    40381 tatatgacag aattaacttg tcagacatgt aaaggttatc gattgaatcc ccaagctcta
    40441 tctgttcaaa tcaatggaac gaatatcggg caaacaaatg agttgtctat tcaaaaagca
    40501 gtagatttct tctcaaactt aacgttatct gaacaagaga aagtaattgc caaaccgatt
    40561 ttaaaagaag taaatgacag actttcattc ttggaaaatg tgggactaga ctatctaact
    40621 ttgagtcgcg cttccggaac attatctggg ggagaagcgc agcgaatcag attggctaca
    40681 caaattggat cgaatctttc tggagttttg tatattttag atgaaccatc gatcggattg
    40741 catcaacgcg acaacgatcg actgattggc tcattaaaga aaatgcgtga tcttggcaac
    40801 accctcgttg tagtcgaaca tgatgaagat acgatgtggg cagcagatta tttgattgac
    40861 gtcggacctg gggccggaga acaaggcgga caaatcgtgg ctgcaggcac tcctgaagaa
    40921 gtagctaatg atgaaaattc tttgacaggg aaatatcttt cagggaaaaa atcaatccct
    40981 gtaccaaaag aacgccgtaa aggtaatggg aaagcaatca agatcactgg tgcttcagaa
    41041 aacaatctga aaaatattag tgttgaattt ccattaggag aatttgtagc agtcacaggt
    41101 gtgtctggtt ctggaaagag tacattggtc aatagtatct taaagaaatc gttagcgcaa
    41161 aaattaaata agaattctgc taagccaggt aaattcaaga caatttccgg ctacgaaagt
    41221 atcgaaaaga tcatcgatat cgatcaaagc ccaatcggcc ggacgccgag aagtaatcca
    41281 gcgacttata caagtgtatt tgatgatatc cgtgggttat ttgctcaaac gaacgaggca
    41341 aaaatgcggg gttataagaa agggcgtttt agtttcaacg taaaaggcgg tcgttgtgaa
    41401 gcttgtcgcg gggatggaat tattaagatc gaaatgcact ttttgcctga tgtctatgtt
    41461 ccttgtgaag tatgtcatgg taaacgatat aattctgaaa cattagaagt gcattacaaa
    41521 ggaaaaagca ttgctgatat tttggaaatg acagtagagg atgctgtaga attcttcaag
    41581 cacattccaa agattcatcg caaactgcaa acgattgttg atgttggctt aggttatgtg
    41641 acgatggggc aaccagcaac gacattgtcc ggtggtgagg cacaacggat gaaacttgcc
    41701 agtgaattgc acaaaatctc taatggaaag aatttctata tactagatga accaacgaca
    41761 ggacttcata gcgatgacat cgcccgcttg ttgcatgtat tacaaagatt agtagatgct
    41821 ggtaacacag ttttagtgat tgaacacaat ctagatgtaa tcaaaacagc agattatatc
    41881 attgatttag gaccagaagg tggagaaggt ggaggaacga tccttgcgac tggaacacca
    41941 gaagaaatca ttaacgtaaa agaaagttat acaggtcact atttgaaaaa aataatggta
    42001 taaagaggat agatagctga gatagttaaa aaaacataaa aaaatgaatt tttgcttgtt
    42061 ttattaatat cagtgttcta gaatcattac atggtagttc tttctacact gattggtata
    42121 gataaaaact tcctataagt tcaatccgtt caaaaaagaa ttgagcaatc ggaggacttt
    42181 cctttgattg cttttttctt tatggtatga tattccttgt gtagttggaa aagaaaaaca
    42241 agagattatg agtaagtgag agataatgat gaacagtatt gaaaatgaag aatttatccc
    42301 tatattatta ggaagcgaca tgaacgttta tggtatggca cgttctttta atgaagcata
    42361 cggcaaaatc tgccaagcat atgcttcgga tcagttagcc cctacccgct acagtaaaat
    42421 tgtcaatgta gaagttattc caggatttga taaagaccct gtatttatcg agacgatgct
    42481 tcgtttggca aaagaaagat attctgataa gagtaaaaaa tatcttttga ttgcttgtgg
    42541 tgatggatat gcagaattga tctcccaaca taaacaagag ctgtcagaat attttatctg
    42601 tccatatatt gattattctt tatttgaaag gctgatcaat aaagtaagct tttatgaagt
    42661 ttgtgaagaa tatgatcttc cgtatcctaa aacattgatc gtcagagaag aaatgttagt
    42721 aaatggacat ttggaacagg aattgccttt tgaattccca gttgctttga aacctgcaaa
    42781 cagtgtggag tatctttctg tccaatttga aggaagaaaa aaagctttta ttttagagac
    42841 tagagaagag ttcgatttaa ttttaggccg gatatacgaa gccggttata aaagtgaaat
    42901 gattgtacaa gattttatcc cgggtgatga tagtaatatg cgtgtgctaa atgcgtatgt
    42961 agatgaagat catcaagttc ggatgatgtg ccttggtcat ccactattag aagatcctac
    43021 accagcatcg attggtaatt acgttgtgat catgccagat tataacgaaa aaatctatca
    43081 aaccattaag gcatttttgg aaaaaatcga atatacaggc tttgccaatt tcgatatgaa
    43141 gtatgatcct agagatggag aatataaatt atttgaaatc aatttgagac aaggtcgtag
    43201 cagtttcttt gtgacattga acggcttgaa tctggctcgt tttgtcacag aagatcgagt
    43261 gttcaacaaa ccctttgttg aaacgaccta tggaacgaat caatcagata aagcaagatt
    43321 atggatgggt gtgcccaaaa aaatcttttt agaatatgct cgggaaaatg aagacaaaaa
    43381 actagctgag caaatgatca aagaaaatcg ttacggtact actgtatttt atgaaaaaga
    43441 ccgatcaatc aagcgctggt tgttgatgaa atatatgttc cataactata ttccaagatt
    43501 caaaaagtat tttcatgtga aagaaggcta gttcatggag aattttttca gtattttagg
    43561 cggaatgggc acgatggcga cagaaagttt tgttcgcttg atcaaccacc gaacgaaagc
    43621 tacaaaagat caagaatatt tgaattatgt cttattcaat catgcaacag ttcctgatcg
    43681 tacagcttat attttagatc gatcagaaga aaatccaatg ccgtttctgc tggatgatat
    43741 tgagaaacaa aatttattgc gaccaaactt cattgtgttg acttgtaaca ccgcacacta
    43801 cttttttgaa gagttacaag cggcaacgga tattcctatc ttgcatatgc caagagaagc
    43861 agcaaatgaa cttgtccgtc agcatacgac agggagagta gcgattctag gaacagaagg
    43921 aagtatgaaa gctggaatct atgaacgcga agtcaaaaat cttggtttcg aaacagtgat
    43981 tcctgatact gcgcttcaag aaaaaatcaa ttacctgatc tatcatgaga tcaaggaatc
    44041 ggattatttg aatcaagagc tctattacga aatcttagag gaagcggtag agcgcctaaa
    44101 ctgtgaaaaa gtcatcctag gttgcacaga attatctttg atgcatgaat ttgctgaaga
    44161 taatcattac ccagtgatcg atgctcaatc tattttagcg gatcgaacga tcgaacgtgc
    44221 attagctgaa agaagcgagg cattggatac agcatcggaa aagtaaaaaa attggatttg
    44281 gggcaaatat cctaaaagat atttggtcca aatctttttg taaaagaaaa agaaaactgg
    44341 taatgatacg cccgggatga tataatttta ttatgcacaa tgtgagaggg gttcgtaaat
    44401 atgacagata atttgcaatt agtcatcata acaggtatga gtggtgcagg gaaaacagta
    44461 gctatccaaa gttttgaaga tatgggctat ttttgtatcg ataatatgcc gcctcgattg
    44521 attccaaaat tctgggaatt gatcaaagaa tcaggtaaag tcacaaaaat ggctttagga
    44581 gtggatttgc gttctcgttc cttctttgaa gaaatccaaa acatgctgat cgaaatcgaa
    44641 aatacgaact tcatcgacac ttctatctta tttttagatg cttcagacga agaactagtg
    44701 gctcgctaca aagaaacaag acgagcacat ccaatggcga tggacggttt gatcactgaa
    44761 gggatacgta aagaacgggc gatactagac gacttgaaag cccaagcttc cgtgatcatt
    44821 gatacgacaa cattgacacc acgacaatta cgtgaaaaaa tcaatgagtc attcaaatca
    44881 aagggagacc aaggatttcg gatcgaattt gtatcatttg gttttaaata cgggttgccg
    44941 atcgattcgg atattgtgat ggatgtgcgt tttttgccca accctcatta tatcccggaa
    45001 ctaagaccat tgaccggaaa agatcagcca gtctatgatt atgtgatgtc ctttgatgaa
    45061 acgcaatcat tctaccataa atttttggat cttctggaaa ctgtattgcc tggatacatt
    45121 aaagaaggaa aaagcagctt gacagttgca atcggctgta caggcggtca acaccgttct
    45181 gtggcattga ctgaacgttt ggcaaatgct ttagggaaaa actataaagt caatgtgaca
    45241 catcgggaca aggataaaag gaaagagacg gtgaaccggt cgtgaaaatg aaaacctacc
    45301 ggataagaaa accaaagatc gtagtggtcg gcggtggcac tggtctgcca gttatcctga
    45361 aaagtttacg aaatcaagga gcagatatca cagcagtagt gactgttgca gatgatggtg
    45421 gaagcagcgg tgcaatcaga gaatcgatcg ctatggctcc tcctggtgac ttgagaaatg
    45481 tcttagtggc gttatcggat atgccacaat tctatgaaga tattttccaa taccgtttca
    45541 aaaaggaaga tcagttttta gccaatcata cgatcggtaa tctgatcatt gccgcagttt
    45601 ccgagatgcg gggaagcact tatgaggcga ttcaattatt agcaaaaatg atgcatgtag
    45661 atggacatgt ttatccttct tcagaaactc ctttgacctt gcatgctgtt tttaaagacg
    45721 gaacgactgc agtaggagaa tctaaaatcg cgatcaaccg taaaaccatt gaccgtgtgt
    45781 atgtctgcaa cacaaatgat aaaagtgaag ccaaagcggc tcgaaaagtc gtttcagcaa
    45841 ttatggacgc cgacatggtc gttttgggac ccggaagttt attcaccagt atccttccaa
    45901 atttagtgat accagaaatc ggcgaagcaa tgctcaagac aactgctgaa accgtctata
    45961 tctgtaatat catgacccaa aaaggggaaa cggaacattt cacagatgcc gatcatatac
    46021 gtgttttaca caagcatttg aatcagcctt ttatcgatac agtattggtc aatacggaaa
    46081 aagtgcctga agattatatg gacccagaga tctatgatga gtatctagta caagtcgcac
    46141 acgacttcaa cggattgaga aatgaaggat gtcgagtgat ttccaccgac tttttagaat
    46201 tgaaagatgg tggtgtcttc cacgatggag acaaagtagt agaagaattg ttccgtttag
    46261 ttttcggttc aaaatattaa gcaatttatt gttggagaaa ggaggagaat aggatgtcat
    46321 ttgctgcaga tgtgaagaaa gaactaactg gtttggaagt tcatcgagaa catgcgaaag
    46381 cggaattagc tgctttgctg cgaatgaacg gctctttagg tattgtgaac cagcagttta
    46441 ttttgaacgt ccaaacagaa aatgctgcaa ttgccagacg tatctattct cttttgaaag
    46501 atcattatca ggtacgaagt gagctactcg ttcgtaaaaa aatgaaatta aaaaagaaca
    46561 atgtctatat cgtccggttg aagcaaggca caaaagatgt gttgatggat cttgatatca
    46621 tggatggtat gatgttcaat ggacacgttt cggacgagat tatgggaaat tcgcaaaaaa
    46681 tgcgttctta cttacgtggt gcttttatgg cctcaggttc agtcaataat ccggaaacga
    46741 gtcgttatca tttggaaata ttttctattt atgaacagca taaccaagac atttgcgata
    46801 tgttaaatta ttacggtcta catgctcgaa cgcttgaacg gcgcaatggc tatatttcgt
    46861 atttaaaagg agcagaaaaa attgctgatt tcttaacatt gattggtgca accaactcaa
    46921 tgctccgatt tgaagatgtt cgaatcgtac gggatatgag aaattcggtc aatcgtctag
    46981 tcaattgtga gacagcaaat ctgaacaaga cgattgatgc ggcttctaaa caaatcgaaa
    47041 acatcgaatt gatcgaagca aaggtcggtc tacatgccct tcctgaaaaa cttcaggaaa
    47101 tcgcagaact tcggcttcaa catccagagg tcagcttgaa agaattaggg gaaatgattc
    47161 cctcaggtgc aatatcaaaa tcgggaatta atcaccgaat tcgcaaaata aatgaatttg
    47221 cggaaaaact gcgtgaagaa gcagtttaat ccaaagaaaa aactgataca tgaagcagga
    47281 gatgcgaatg tatcagtttt ttttaaaagc tatacttgct tttcttccgt caaactataa
    47341 ttccaacttt actggagtaa cataatgttg aaaggcttct attgtttttt aatacaatcg
    47401 tataaaaagc ggggagacaa ctgaatgaag attttagttt tatacataaa cttttgctta
    47461 atatagaagt cctgatatag ataggatcaa gtatttatct ataactggag attaattaaa
    47521 aactggaagg aactgctcga tcaaccttga gaactgtttt tcttacttct ggtcaagatt
    47581 ctcgtggttg gtggataagg atggttgata agttcattgg attagctagt cgaaaacaac
    47641 caggtgtaat taatgtagaa gttttaacaa ggggatggag tgatttattc agtgataatc
    47701 ataagttcat aaatagtaat tgggatgtat ggttggtata cgttatacct gaacttgaac
    47761 tatcaagatt tggtttagaa tttgagaatc gatgtttata aagggagggc aaaaaatgtg
    47821 cttttttaca acttttacta tcattatgat tgcagtactt tttatttttc tagatatagc
    47881 aaagcgaaat acagcatttt tattatatcg ggttttactt cgagcaggtc tcataacatt
    47941 tatatcgatc gttggatttt ttctattcac agttattgta tttatttgga gaactacagc
    48001 accaccattg ccagagatta catatggaga atttccgttt agtcttgaat atgaaataga
    48061 tgaggaaaga attttgattg aagatacggt gattgcagaa tttataggaa gttttagagg
    48121 gaatgtgaca actcaaccta gacgaagttg gagtataaaa cttttaaata atagtggaga
    48181 gttgaaagca tttcattatg aattcttaat aatagaaagt gaagatgtaa caatcacttt
    48241 aagtagtggc tctgctggat attttatgaa tgatggtgga agcaagccac agctcacagt
    48301 ccgtgatcgt attgcaggtt ttatgcattt ttctcactat gacttagaag aagctcgtaa
    48361 aatacttgca gaatatggga tcacattaat caattgggaa tatgcgccac cgataagaaa
    48421 ttttttcaat gattattatt agtcttataa aaaacagaat gtaaaattta tgtgcgcgag
    48481 tatttagttt tccatttttt gtttgataga tatatagcat tgttgaagca tgggatagtg
    48541 gtgccaattt tatcaatgag gttggtcagg cagttaacat aattggtgat gctataaatc
    48601 catttaattg gaattggagg aattaggtgt ggaaacagtg agattatgga tattcatgaa
    48661 aaattttcgt tcggctttga gatatgttat tctggaatca aaatcaggtt attatctgat
    48721 tgacacagat cactatactt tttggggagg ctaccttttt ccgattttta attggataat
    48781 cccccataag gttatgaaaa ttaacttgcc tgcagaagaa ataaatagat taatactcaa
    48841 caaagattta caaaggaaaa gagagaaaga acattctaat gttgcttgga taggtccagc
    48901 atttgcagta gtgacttctt caattgtcgg tcctatgcta gagagtattt atagtgattt
    48961 ttctattgga atacgttatt tatttgttac tttaagtata gctgttcttt tgtgcataaa
    49021 gggttatgtt agcaagaaga ataaagatat tttaacacta atcggtgtaa acaatttatc
    49081 tagtactaaa attatactgt ttcctgaatc tataaaagaa ggatcaaggt tgttgataat
    49141 ttttataatg cttatactgt tttctgtttt atttggggtg atgtttatag a
//
LOCUS       DARBER010000008        44923 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARBER010000008 DARBER010000000
VERSION     DARBER010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.008"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(2..1012)
                     /locus_tag="SCK06_000507"
     CDS             complement(2..1012)
                     /locus_tag="SCK06_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1150536.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="SCK06_000508"
     CDS             complement(1192..1536)
                     /locus_tag="SCK06_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1150537.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="SCK06_000509"
     CDS             complement(1529..1963)
                     /locus_tag="SCK06_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG1150538.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="SCK06_000510"
     CDS             2101..2838
                     /locus_tag="SCK06_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150539.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="SCK06_000511"
     CDS             2831..4042
                     /locus_tag="SCK06_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150540.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4246..5028
                     /locus_tag="SCK06_000512"
     CDS             4246..5028
                     /locus_tag="SCK06_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG1150541.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="SCK06_000513"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="SCK06_000513"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG1150542.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="SCK06_000514"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="SCK06_000514"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG1150543.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="tadA"
                     /locus_tag="SCK06_000515"
     CDS             7744..8673
                     /gene="tadA"
                     /locus_tag="SCK06_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG1150544.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /locus_tag="SCK06_000516"
     CDS             8630..9679
                     /locus_tag="SCK06_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG1150545.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /locus_tag="SCK06_000517"
     CDS             9676..9954
                     /locus_tag="SCK06_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG1150546.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /locus_tag="SCK06_000518"
     CDS             9929..10399
                     /locus_tag="SCK06_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG1150547.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10416..10718
                     /locus_tag="SCK06_000519"
     CDS             10416..10718
                     /locus_tag="SCK06_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150548.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10735..11106
                     /locus_tag="SCK06_000520"
     CDS             10735..11106
                     /locus_tag="SCK06_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG1150549.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /locus_tag="SCK06_000521"
     CDS             11078..11437
                     /locus_tag="SCK06_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150550.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="SCK06_000522"
     CDS             11609..12616
                     /locus_tag="SCK06_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1150551.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="SCK06_000523"
     CDS             12641..13825
                     /locus_tag="SCK06_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG1150552.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /locus_tag="SCK06_000524"
     CDS             complement(14262..15845)
                     /locus_tag="SCK06_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1150553.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16423..16566)
                     /locus_tag="SCK06_000525"
     CDS             complement(16423..16566)
                     /locus_tag="SCK06_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG1150554.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="SCK06_000526"
     CDS             complement(16789..17268)
                     /locus_tag="SCK06_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1150555.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="SCK06_000527"
     CDS             complement(17341..17625)
                     /locus_tag="SCK06_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150556.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="SCK06_000528"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SCK06_000528"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="SCK06_000529"
     CDS             complement(17969..19255)
                     /locus_tag="SCK06_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG1150557.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="SCK06_000530"
     CDS             19393..20064
                     /locus_tag="SCK06_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG1150558.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="SCK06_000531"
     CDS             20030..20518
                     /locus_tag="SCK06_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG1150559.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="SCK06_000532"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="SCK06_000532"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG1150560.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="SCK06_000533"
     CDS             21479..22243
                     /locus_tag="SCK06_000533"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG1150561.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="SCK06_000534"
     CDS             22476..24689
                     /locus_tag="SCK06_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG1150562.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /locus_tag="SCK06_000535"
     CDS             24700..25146
                     /locus_tag="SCK06_000535"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG1150563.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="SCK06_000536"
     CDS             25317..26576
                     /locus_tag="SCK06_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG1150564.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="SCK06_000537"
     CDS             complement(26665..27612)
                     /locus_tag="SCK06_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1150565.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /locus_tag="SCK06_000538"
     CDS             28006..29298
                     /locus_tag="SCK06_000538"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG1150566.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="SCK06_000539"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="SCK06_000539"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG1150567.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="SCK06_000540"
     CDS             31191..32066
                     /locus_tag="SCK06_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1150568.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="SCK06_000541"
     CDS             32332..34215
                     /locus_tag="SCK06_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1150569.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="SCK06_000542"
     CDS             complement(34290..34574)
                     /locus_tag="SCK06_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1150570.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="SCK06_000543"
     CDS             complement(34564..35298)
                     /locus_tag="SCK06_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG1150571.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="SCK06_000544"
     CDS             35427..36050
                     /locus_tag="SCK06_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG1150572.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="SCK06_000545"
     CDS             36242..37363
                     /locus_tag="SCK06_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG1150573.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="SCK06_000546"
     CDS             37370..40498
                     /locus_tag="SCK06_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG1150574.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="SCK06_000547"
     CDS             40517..43711
                     /locus_tag="SCK06_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG1150575.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="SCK06_000548"
     CDS             complement(43857..44564)
                     /locus_tag="SCK06_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1150576.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14416 a         7969 c         9413 g        13125 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgcttac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag acaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    44701 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    44761 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    44821 agatttattt ttttaataca gtctcaatat aactattcaa acataaaata acgttatttg
    44881 atttatttgt ttatagtaaa ttttatttag taatcattgc ttt
//
LOCUS       DARBER010000009        44860 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARBER010000009 DARBER010000000
VERSION     DARBER010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.009"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..604
                     /locus_tag="SCK06_000549"
     CDS             212..604
                     /locus_tag="SCK06_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1150577.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="SCK06_000550"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="SCK06_000550"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG1150578.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="SCK06_000551"
     CDS             3165..4775
                     /locus_tag="SCK06_000551"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG1150579.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="SCK06_000552"
     CDS             5067..5936
                     /locus_tag="SCK06_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG1150580.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="SCK06_000553"
     CDS             6101..7372
                     /locus_tag="SCK06_000553"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1150581.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="SCK06_000554"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="SCK06_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG1150582.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="SCK06_000555"
     CDS             8763..9026
                     /locus_tag="SCK06_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG1150583.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="SCK06_000556"
     CDS             9471..9920
                     /locus_tag="SCK06_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1150584.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="SCK06_000557"
     CDS             9977..11395
                     /locus_tag="SCK06_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1150585.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="SCK06_000558"
     CDS             11395..12255
                     /locus_tag="SCK06_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG1150586.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="SCK06_000559"
     CDS             12429..13202
                     /locus_tag="SCK06_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1150587.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="SCK06_000560"
     CDS             13349..13714
                     /locus_tag="SCK06_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG1150588.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="SCK06_000561"
     CDS             complement(13862..15070)
                     /locus_tag="SCK06_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1150589.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="SCK06_000562"
     CDS             complement(15089..15367)
                     /locus_tag="SCK06_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150590.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="SCK06_000563"
     CDS             15521..16216
                     /locus_tag="SCK06_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1150591.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="SCK06_000564"
     CDS             complement(16658..17455)
                     /locus_tag="SCK06_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG1150592.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="SCK06_000565"
     CDS             complement(17452..18636)
                     /locus_tag="SCK06_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1150593.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="SCK06_000566"
     CDS             complement(18661..19908)
                     /locus_tag="SCK06_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1150594.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="SCK06_000567"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="SCK06_000567"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG1150595.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="SCK06_000568"
     CDS             21888..22202
                     /locus_tag="SCK06_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG1150596.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="SCK06_000569"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="SCK06_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG1150597.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="SCK06_000570"
     CDS             22887..23531
                     /locus_tag="SCK06_000570"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG1150598.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="SCK06_000571"
     CDS             23546..23875
                     /locus_tag="SCK06_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG1150599.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="SCK06_000572"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="SCK06_000572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG1150600.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="SCK06_000573"
     CDS             24863..25687
                     /locus_tag="SCK06_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG1150601.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="SCK06_000574"
     CDS             25680..26027
                     /locus_tag="SCK06_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG1150602.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="SCK06_000575"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="SCK06_000575"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG1150603.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="SCK06_000576"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="SCK06_000576"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG1150604.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCK06_000577"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCK06_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG1150605.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCK06_000578"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCK06_000578"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG1150606.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="SCK06_000579"
     CDS             31657..32451
                     /locus_tag="SCK06_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150607.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="SCK06_000580"
     CDS             32441..34483
                     /locus_tag="SCK06_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150608.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="SCK06_000581"
     CDS             34764..34913
                     /locus_tag="SCK06_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG1150609.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="SCK06_000582"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="SCK06_000582"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG1150610.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="SCK06_000583"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="SCK06_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG1150611.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="SCK06_000584"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="SCK06_000584"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG1150612.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="SCK06_000585"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="SCK06_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG1150613.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="SCK06_000586"
     CDS             39330..40412
                     /locus_tag="SCK06_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG1150614.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="SCK06_000587"
     CDS             40412..41215
                     /locus_tag="SCK06_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1150615.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="SCK06_000588"
     CDS             41285..42025
                     /locus_tag="SCK06_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1150616.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="SCK06_000589"
     CDS             42036..43040
                     /locus_tag="SCK06_000589"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG1150617.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="SCK06_000590"
     CDS             complement(43085..44782)
                     /locus_tag="SCK06_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150618.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14422 a         7827 c         9341 g        13270 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat
//
LOCUS       DARBER010000010        44401 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARBER010000010 DARBER010000000
VERSION     DARBER010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.010"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            130..777
                     /locus_tag="SCK06_000591"
     CDS             130..777
                     /locus_tag="SCK06_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG1150619.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            792..2318
                     /locus_tag="SCK06_000592"
     CDS             792..2318
                     /locus_tag="SCK06_000592"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG1150620.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2367..5165)
                     /gene="ileS"
                     /locus_tag="SCK06_000593"
     CDS             complement(2367..5165)
                     /gene="ileS"
                     /locus_tag="SCK06_000593"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG1150621.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5427..6134)
                     /locus_tag="SCK06_000594"
     CDS             complement(5427..6134)
                     /locus_tag="SCK06_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG1150622.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6155..6937)
                     /locus_tag="SCK06_000595"
     CDS             complement(6155..6937)
                     /locus_tag="SCK06_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG1150623.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(6986..7273)
                     /locus_tag="SCK06_000596"
     CDS             complement(6986..7273)
                     /locus_tag="SCK06_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG1150624.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7288..7887)
                     /locus_tag="SCK06_000597"
     CDS             complement(7288..7887)
                     /locus_tag="SCK06_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG1150625.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7901..8578)
                     /locus_tag="SCK06_000598"
     CDS             complement(7901..8578)
                     /locus_tag="SCK06_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG1150626.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8595..9836)
                     /gene="ftsZ"
                     /locus_tag="SCK06_000599"
     CDS             complement(8595..9836)
                     /gene="ftsZ"
                     /locus_tag="SCK06_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG1150627.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9859..11184)
                     /gene="ftsA"
                     /locus_tag="SCK06_000600"
     CDS             complement(9859..11184)
                     /gene="ftsA"
                     /locus_tag="SCK06_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG1150628.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11340..12560)
                     /locus_tag="SCK06_000601"
     CDS             complement(11340..12560)
                     /locus_tag="SCK06_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG1150629.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12575..13663)
                     /gene="murG"
                     /locus_tag="SCK06_000602"
     CDS             complement(12575..13663)
                     /gene="murG"
                     /locus_tag="SCK06_000602"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG1150630.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13689..15050)
                     /locus_tag="SCK06_000603"
     CDS             complement(13689..15050)
                     /locus_tag="SCK06_000603"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG1150631.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15064..16026)
                     /locus_tag="SCK06_000604"
     CDS             complement(15064..16026)
                     /locus_tag="SCK06_000604"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG1150632.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16052..18244)
                     /locus_tag="SCK06_000605"
     CDS             complement(16052..18244)
                     /locus_tag="SCK06_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1150633.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18245..18646)
                     /gene="ftsL"
                     /locus_tag="SCK06_000606"
     CDS             complement(18245..18646)
                     /gene="ftsL"
                     /locus_tag="SCK06_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG1150634.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18651..19610)
                     /gene="rsmH"
                     /locus_tag="SCK06_000607"
     CDS             complement(18651..19610)
                     /gene="rsmH"
                     /locus_tag="SCK06_000607"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG1150635.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19631..20062)
                     /gene="mraZ"
                     /locus_tag="SCK06_000608"
     CDS             complement(19631..20062)
                     /gene="mraZ"
                     /locus_tag="SCK06_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG1150636.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20232..20600)
                     /locus_tag="SCK06_000609"
     CDS             complement(20232..20600)
                     /locus_tag="SCK06_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG1150637.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20771..21727
                     /locus_tag="SCK06_000610"
     CDS             20771..21727
                     /locus_tag="SCK06_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1150638.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21800..21916
                     /locus_tag="SCK06_000611"
     CDS             21800..21916
                     /locus_tag="SCK06_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG1150639.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21971..23653)
                     /gene="recN"
                     /locus_tag="SCK06_000612"
     CDS             complement(21971..23653)
                     /gene="recN"
                     /locus_tag="SCK06_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG1150640.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23671..24120)
                     /locus_tag="SCK06_000613"
     CDS             complement(23671..24120)
                     /locus_tag="SCK06_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1150641.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24227..25045)
                     /locus_tag="SCK06_000614"
     CDS             complement(24227..25045)
                     /locus_tag="SCK06_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG1150642.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25054..25944)
                     /locus_tag="SCK06_000615"
     CDS             complement(25054..25944)
                     /locus_tag="SCK06_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1150643.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25946..26170)
                     /locus_tag="SCK06_000616"
     CDS             complement(25946..26170)
                     /locus_tag="SCK06_000616"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG1150644.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26175..27512)
                     /locus_tag="SCK06_000617"
     CDS             complement(26175..27512)
                     /locus_tag="SCK06_000617"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG1150645.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27513..28367)
                     /locus_tag="SCK06_000618"
     CDS             complement(27513..28367)
                     /locus_tag="SCK06_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG1150646.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28464..28913)
                     /gene="nusB"
                     /locus_tag="SCK06_000619"
     CDS             complement(28464..28913)
                     /gene="nusB"
                     /locus_tag="SCK06_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG1150647.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28906..29331)
                     /locus_tag="SCK06_000620"
     CDS             complement(28906..29331)
                     /locus_tag="SCK06_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1150648.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29577..30641)
                     /locus_tag="SCK06_000621"
     CDS             complement(29577..30641)
                     /locus_tag="SCK06_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1150649.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30813..31058)
                     /locus_tag="SCK06_000622"
     CDS             complement(30813..31058)
                     /locus_tag="SCK06_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1150650.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31197..31571)
                     /locus_tag="SCK06_000623"
     CDS             complement(31197..31571)
                     /locus_tag="SCK06_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG1150651.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31654..32568)
                     /locus_tag="SCK06_000624"
     CDS             complement(31654..32568)
                     /locus_tag="SCK06_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG1150652.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32589..33392)
                     /locus_tag="SCK06_000625"
     CDS             complement(32589..33392)
                     /locus_tag="SCK06_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG1150653.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33425..34414)
                     /gene="manX"
                     /locus_tag="SCK06_000626"
     CDS             complement(33425..34414)
                     /gene="manX"
                     /locus_tag="SCK06_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG1150654.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34517..35008)
                     /locus_tag="SCK06_000627"
     CDS             complement(34517..35008)
                     /locus_tag="SCK06_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1150655.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35199..38006)
                     /locus_tag="SCK06_000628"
     CDS             complement(35199..38006)
                     /locus_tag="SCK06_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1150656.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38440..38733)
                     /gene="rpmA"
                     /locus_tag="SCK06_000629"
     CDS             complement(38440..38733)
                     /gene="rpmA"
                     /locus_tag="SCK06_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG1150657.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38746..39090)
                     /locus_tag="SCK06_000630"
     CDS             complement(38746..39090)
                     /locus_tag="SCK06_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG1150658.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39117..39425)
                     /gene="rplU"
                     /locus_tag="SCK06_000631"
     CDS             complement(39117..39425)
                     /gene="rplU"
                     /locus_tag="SCK06_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG1150659.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39445..39521)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39707..40348
                     /locus_tag="SCK06_000632"
     CDS             39707..40348
                     /locus_tag="SCK06_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1150660.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40410..42041)
                     /locus_tag="SCK06_000633"
     CDS             complement(40410..42041)
                     /locus_tag="SCK06_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG1150661.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(42164..42523)
                     /locus_tag="SCK06_000634"
     CDS             complement(42164..42523)
                     /locus_tag="SCK06_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG1150662.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(42682..42755)
                     /locus_tag="SCK06_000635"
     tRNA            complement(42682..42755)
                     /locus_tag="SCK06_000635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42719..42721),aa:Arg,seq:tct)
     gene            complement(43064..43747)
                     /locus_tag="SCK06_000636"
     CDS             complement(43064..43747)
                     /locus_tag="SCK06_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG1150663.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(43875..44393)
                     /locus_tag="SCK06_000637"
     CDS             complement(43875..44393)
                     /locus_tag="SCK06_000637"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1150664.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT        12578 a         9627 c         7509 g        14687 t
ORIGIN      
        1 atcatctttc gttttttgtt gtaaaaacgt ctaaaaatcc tttgtttttt atttagaaaa
       61 atagtcaatt aacgtttttt ttgttacact tactaatgat ggttttttac catgaaagga
      121 tgatcgaata tgacaccaaa tcgagaagat tatttgaaaa tcattttaga actaggtgga
      181 gatacaacga aagtcaacaa taaacaaatc gtatctagtc tagatgtctc agctgcatct
      241 gtcagtgaaa tgatttctaa acttgtgaaa gaagagcttg tagaacattc cccttatcaa
      301 ggtgtacaat tgactgcaag cggactgcaa aaagctagca gtctgattcg caaacatcgc
      361 ttatgggaag tttttttagt tgaacattta gactatagct ggaatgaagt ccatgatgat
      421 gctgaagtat tggaacatgt gacttcggaa catcttgcag atcatttaga aaactatttg
      481 aatcatcctg aacattgtcc tcatggagga tcgattccta aaaaagagga agttgtccat
      541 gaagaacgcc gacaaacatt ggagtcctat cctgtcggta caaaagtgcg tatcgcacga
      601 gtgcttgatg aaaaagaatt gttggattac ttagtcacta ttgacctgaa tatcaacgaa
      661 gaatatgaga tcgttgacat cgcagcttat gaaggaccga tcactatcca aaatgataga
      721 aagaaaatcc ccgtcagctt taaggcggcc agcacgattt ttgtcgataa aatataaagg
      781 agtgtatcca catgtctaat gaaaaaaatg tattgtttac gatattcggc ggtacaggtg
      841 acctagccca gcgtaaattg tacccgtctt tgttccgcct ttacagaaaa ggaaatttag
      901 gcgagcattt tgctgtaatc ggaaccgctc gccgtccctg gagcgatgag cactaccgtg
      961 aagttgtcaa agaaacaatc cgtgcattga atccttcaga tgaagaagca actacctttg
     1021 caagccattt ttactatcaa tcccatgatg taaatgattc aacgcattat cagacattaa
     1081 aagaattgtc tgaccgatta gatggacact atcaattaaa cggtaaccgt gtctattatt
     1141 tggctatggc acctcaattt tttggaacga tcgtttctca tttgaaatca caaaatatta
     1201 tgacaaaaaa tggcttcaat cgtttgatca tagaaaaacc gttcggttct gattaccagt
     1261 ctgcttttga attgaaccag caaatcagag aagtattccc agaacaagat attttcagga
     1321 ttgatcatta tttaggaaaa gaaatgatcc aaaacatctc agctatccgt tttgccaata
     1381 atatttttga ggctcaatgg aacaatcgct atatcgacaa tatccaaatt acttttggcg
     1441 aaaacctcgg tgtagaagat cgcgggggct actacgatca tagtggcgca cttaaagaca
     1501 tggtgcaaaa tcatatttta caggttgtag cattacttgc aatggaaccg ccagtagcat
     1561 tttccgaaaa agaaatccgt acagaaaaga tcaaagcatt gaaagctatc cgcatttata
     1621 acgaagaaga agtgttagaa aacttcgttc gcgggcaata tgctcaaggt gaattggatg
     1681 gtgtacaatt caagggctat cgcgaagaag acaaagtgga tgctcaatca gagactgaaa
     1741 cattcgtcgc aggcaaattc actatcgata acttccgttg gtcaggtgtt cctttctatg
     1801 tacgtacagg caaacgtttg actgaaaaag ggactcggat caacatcgta ttcaaacaag
     1861 ttcctgttaa tgtattcaaa acatctgtcg atgaaccatg tgacgatacg acacttccac
     1921 ctaatgttct aacgatctat attcagccaa ctgaaggatt ttctttatcc ttgaatggga
     1981 aagaagtagg gcaaggattt gaaacggaac caatcaaatt ggaatttaga aatagtgcag
     2041 aaatggttga aaatagccca gaagcttacg aaaaattact attagatgcg ttgaatggtg
     2101 atggaacgaa cttctctcat tgggaagaag tcgctcaatc ttggcatatc gtggatgtca
     2161 ttcgaaaagc ttgggaccaa acacaaccgg attttcccaa ctacaaagct ggatcaatgg
     2221 gacctaaagc agctttcgaa ctacttgaaa aagacggatt cgaatggatc tggcaaccag
     2281 ataactggta tcgtgaacgt ggaaaattaa acgattgata acaaaagggg gtgtgacaaa
     2341 gaacatgtca cacccccttt tcccttttac ttttcttcga atccttcagc tacagcttca
     2401 ggatagaagt cttcaacgat ttttgcacaa cgaccgcata gatgaggaag tttttcatct
     2461 actcctacat ctttacgaac ttggcgacag cgatcgcaag tttccccgtc tgctttttcc
     2521 actgtaatag caacatcatc aaataccata gcgttatctg gcacttgttc atctgctgcc
     2581 ataacttcaa agtagtctgg tgaaacgatc aataattgtg caatatctgc atccaaagct
     2641 tttaacattt cacgaacttg ttgttttgga taaacagtta gttttgcttc caaagattta
     2701 ccaatcaatt tgctgtttct tgcttcttcc aatgctttta agacattatc acggaagtcc
     2761 atgaaagcag tccaggtatc catcaattct tcttggtttg caaactcttc ataaccagga
     2821 aactcgctta actgaacgta ctcctcttct tctttcagga atgaccagat ttcttctgct
     2881 gtatgcggaa tgataggagt caataatttt gtcaaagcta cagctgtttg atagaaaaca
     2941 gtttgcatgc aacgacgttg ataatcatct tctgcttcaa tataaactac atctttagca
     3001 aaatccaggt agaatgacga tagatcaacc gtcaagaagt tcataactgt acggtaaata
     3061 tgcatgaagt tatatttgtc gtacccttct tctcggattt ctttgattac ttggttcaat
     3121 cgaacggtca tatatttgtc tacagaacgt aaatcattgt acgaaacagt atctttttta
     3181 ggatcgaaat cttctgtatt cgctaataag aaacgcatcg tatttctgat tttacgatat
     3241 acttctgcca cttgacttaa gatatccata gaaacacgaa catctgcttc ataatctaca
     3301 ctagataccc ataagcgaag gatatctgca ccaaattgtt tgatcacttt ttctggtgca
     3361 atgatgtttc ctaaagattt actcatctta cggccttcac catctaaagc aaatccttga
     3421 gaaataactg atttgtatgg tgctacacca ttgatagcca cactagtcgt gatacttgag
     3481 ttgaaccagc cacgatattg gtctgatcct tctaaataca tatctgctgg gaatgataat
     3541 tctggtcgct gacgtaaaac agcttcatga gaagaaccag aatcaaacca gacatccatg
     3601 atatcttttt cttttgtaaa ttcgccatta ggagaacctg ggtgggtaaa tccttcagga
     3661 agaagttctt tcgcttcttt ttcaaaccag ataattgatc catgttcagc aaataatttt
     3721 gccacatgtt cgattgtttc tggtgtgatg attgcttctc cattttcagc atagaaaatc
     3781 ggaagtggta ctccccatgc acgctgacgg gaaattaccc aatcgccgcg gtcacggatc
     3841 atattataca aacgtgtttt accccatggg ataacccact ctactttttc tacttcatcc
     3901 aaaatattct gacggaattt atcgatagat gcaaaccatt gtggagttgc ccgatagatc
     3961 acaggctttt tcgtccgcca atcgtgagga taactatgag taaagaaatc tagcttcagc
     4021 aaagctcctt tttcttttaa tagatcagtg atcataggat ttgctttgtc ataaaagata
     4081 ccttcaaatc caggtgcttc ttctgtaaac aatccttttg agtctacagg tgacactaca
     4141 gatagtttat atttgctacc agcgatatag tcatcttccc catgtcctgg agcagtatgg
     4201 actaatccag taccagcgtc taaagtaaca tgatcgccta aaatcacaag tgattcacgt
     4261 tcatagaatg gatgacttgc agtcatatat tccatttctt gtccagcaaa ttcttttaga
     4321 actgttacat tttcccaacc gatttctgat tggactgttt ctagcaaatc tttcgctacg
     4381 acaaacttac ggccatcggc ttccaccaat acatacgtaa attttggatt tacagcaata
     4441 ccttggtttg ctggcaatgt ccatggtgtt gtcgtccaaa tgacaaaagc tgtatcttca
     4501 tcaagaatat ctttgccgtc tttgacattg aaagctacaa aaatagatgg agattttaca
     4561 tctttatatt caatttcagc ttccgctaaa gaagactcac ttgacggaga ccagtaaatt
     4621 ggtttcaatc ctttgtaaat atagcctttt tcagccattt taccaaatac gcgtacttgt
     4681 gcagcttcat aagaaggatc taatgtaaca taaggatgtt cccaatctcc tgcaactcct
     4741 aggcgtttga aatctgctcg ttgcttatct acttgggaaa gtgcatattc acgacatttt
     4801 tccaagtatt ctgccatgga catttcttta cgtttcacgc ctttattagc caatacttgt
     4861 tcaatcggca atccatgtgt atcccatcct ggaacatatg gtgcacgata gccagacatt
     4921 gattttgaac gaatgataat gtctttactg attttattca atgaatgtcc taaatggata
     4981 ttaccattgg catatggagg tccatcatga agaacaaagc taggttttcc ttcgttcaac
     5041 tcttgtcttt tttggtagat tcgattttct tcccattctt tttgccactc gccttctcgg
     5101 tttggcaaat taccacgcat agggaatttt gtttttccta attgcaaggt ttctttcatt
     5161 ttcattcaac catacactcc tttatttgtt ttccggacac aaaaaagcat gttcagccgc
     5221 aagggacgaa catgccgtgg taccacccaa atttagttgc atctatctag tgcaaactct
     5281 ctgtgaccaa taacgaggtc agccgctgct tcttactcta atttcggaaa agcaggtctc
     5341 aagtagagga tctttcacca tgcagtgtct taccgaactt ccactctcat cggctcgctt
     5401 taagaataac aaagtgcttt tctgttctac tcgttttcat tttcaccagg cacgatcgag
     5461 atatcaatag cctgtgtatc aaatgccgtc ataggtgctt ctatgactcc tgtgtctgag
     5521 tttacatcag tatcattttc attgtcaagc tgttttgcta aaacttcttt gatcacttca
     5581 tggctgtcag tcacgtagct ggagaatggt tgtaaaattt catcccattc cggacttttc
     5641 acttgttcta attgagactc aagcatcaag ctgatacgtt gatggaatac tcgtgttttt
     5701 ttcttaagat cttcagtttc tgtcgcaagc tgtcttgctt tttctgttgc atctgtcaag
     5761 atttttcttg ctttttcttc tgcatcagtt gttaattgat gtgcacgttt ttcagcgttc
     5821 gcaactaact catctgcttt gttttgtgca gaagtaacga tcacttcgga ttccttgctt
     5881 gcacttgttt ttactttatc agccgtatct tgagctacga tgattgattg gttcaaagca
     5941 tcttttaatt cattgaagta ctcaagtttt tcttcagaat gtttgactgc tttttctaat
     6001 tctcgatttc tttgagtcaa ttcctcatag tcacgaacaa ctaaatctaa gaaatcatct
     6061 acttcgtctt gattatatcc ccgcattttt gtcgggaatg tcttattttg gatatctaat
     6121 ggagttaaag ccatgagtaa accacctttt tattttattt acgtaataca ccgaacagta
     6181 ccctgtactt ttcttttttc gtcttcccct caagttcttg gatctggact ctgccataac
     6241 ctctgactga aataatatct aatagttcaa gtacaaaatc aggtcttgta ttttctgtcc
     6301 agttcacttt tactttacca gcttcgatca gctgcttgga ccgctgtctg gaaatattat
     6361 aaatctcaga gatcacactg tctagtcgca tggaactaac tgttcctctt tcttgtgtcc
     6421 agctatcttt cggcataatc atttctgtgt atttgattgg ttccaagcgt acagcaacct
     6481 ttccaatctt tgttacttgt gcagcaacaa aatgatccac ctcatttgca ataaatactt
     6541 gccagcgatc accgtccgag atgatatcgc caaagtaaga gcgtttgatc cccgttccca
     6601 ctaaagttcc aaggattttc ccatgactta ggctggcaaa tttgctagga taatggattt
     6661 catacaatgc tgtttcaaaa tcttcctcta caggttcgta ataatcgggg aaaatcaaac
     6721 atctttttct ttctgcctgt tcatatccgc cataaaacat gaaacgcaaa tcactgcttt
     6781 gtcgaatcag tgtttctaaa atataggatt gtcttgggtc aagaaattcg gtcagatagg
     6841 gtgcatattg tccttgaacc tgttcaatcc aatcgcctac cgaatcgata aatgtacgtt
     6901 catctgcccg aaagtgctgg tatacatttg catccaaaat tttcccctcc tatctattct
     6961 gagaataaag attttgttgc ttcaatcaat aaactaaatg gacagttatc catataacga
     7021 tctgtgctaa accttgtacg gctaagttca agatcaggat tgcccctaaa attgtgaaat
     7081 ccaagggtcc aatcctcaaa ggcagacgat cgaacaaact gatatacggt tcgcagatct
     7141 tagctaataa tcgtcctagc cctgattgat acccgcctgg aaaccaagag agcaatgcat
     7201 ataccatcaa gataatgcta tagaaatagg ctgcccgttg aagtaaatga agtaatagtg
     7261 caatagtata aatcgtagtt cctcctctta cagatcaaac aaattcgcat ctgcaaaaga
     7321 ttgagcggtt gaactgtcaa tctccatccc agtcggtgta caaagaaaaa tctcatctcc
     7381 aacacgtttg atatctccat ctagtgcata aactgttcct gttagaaaat caacgattct
     7441 tctcgcttgt ttttcttcta ctaaatgaaa attcactaag gttgcttctc cagcaatgat
     7501 tcgtttagca atcgccatag cttcagaata agctcttggt tccattacag tgatttttcc
     7561 agcaagattt gctttaggtt gtgtagaggt tcttgaagct gttctttctt tcgctacagt
     7621 ttttgtaggt gattgatgta gttcaattac ttttttcttt tcagccggct gaatactttc
     7681 atgcgttaca ggacgtgaag tcgccgtttt ttgtttttca tgagtattcg cagcagatac
     7741 agaatgagtt gattgattct gtgttgtatt ttttttagtt ggctgtacag ccttttttgt
     7801 tgtttcagga taatcttggt attcgtactc ttcctcatca gctaatccga aaaaattagc
     7861 aattgctcct ctgtttaaaa gcggcatttt tgttcctcct ttattcgtct ttgaatagcg
     7921 ctgttcctac acggataaat gttgcacctt cttcaatcgc aatttgaaag tcttgactca
     7981 tccccatact taattcacta catggtgcat gggcataacc ttttgcttga acaagatcac
     8041 gcaattcttt cagcgtacca aacactttat ggatttgctc atcagaagct tctttaggcg
     8101 ccatagtcat caacccaaca accttgattt ttgtgcaggc agctaaagat tccacaaacg
     8161 gtaaaagctc atctggtgaa attccatgtt ttgattcctc accagaaaca tttacttcaa
     8221 taaaacagga taattctttc tctaaccgct tctggatttc ttctgctaat cttaaactgt
     8281 ccaacgcatg aaagtaatca atttcattta caatcaattt tacctttctg cgctgtaaat
     8341 taccaattaa atgccacttt atttcaggaa aatcaaataa atcttgtttc ttttctaata
     8401 atttatctac tcggttttct gcacaattca taacgccagc tctaaccaat tcttcagctt
     8461 gagcggattc aacagatttt gttacagcta ctaatgtaac ttctttcgga tcacgatgaa
     8521 caagagcaca cgaatcctga atttcttgct tgattttctg caagttgtca gcaatcatgt
     8581 gctcttcctc tcctttattt tttacgacgg aaaaacggtg gcgtatttaa ttcatcatcg
     8641 ccatgagatt ttgtttcttc gcggttgaat gtatcaaatt cttttttttc gatattgtca
     8701 aaattggaat catcaactct aggacgaaca ttttgttcac gtctgatatc ccaatcgcca
     8761 aaactgtttt cttcttctga agaagttggt ttagcttgat ccatatctaa tacagtcttt
     8821 tgagcaggtg attgcatttg tgcttgtcta gctggtcggc tagacttgcg ttcctttttt
     8881 gattcatcga tacctgttgc gatgaccgtc acacggattt catcgcccat ttcttcattg
     8941 atagatgtac ctagaatgat atttacatcg ccagttgctg catttgctac gatatctgaa
     9001 gcatcttgtg cttcgaacaa tgtcatatcc aaaccgcctg tgatgttaag aagtacttgt
     9061 tcagctccat caatagatgt ttcaagcaat ggagaggaga ttgctttttt cgttgcttct
     9121 actacgcgat cttctccact agccacaccg atccccatca atgctgtacc ttggttttcc
     9181 atcactgttt tcacgtcagc aaagtcaagg ttaacgtaac ctggcgctgt gatcaaatca
     9241 gagatacctt gtacaccttg tcgtaaaaca ttatctgctt cacggaaagc ttcaagcatc
     9301 ggtgtctttt tatctacaac ttctaataaa cggttatttg agataattaa taatgtatcc
     9361 acattttctt tcaaacgagc gataccttct gcagcaaaac ggccacgttt cggtccttca
     9421 aaagtgaatg gacgtgtcac gacaccaaca gtcaatgcgc ccaattcttt cgcgatacct
     9481 gcaacgattg gtgcagcacc tgtaccggtt cctccaccca ttcctgcagt gatgaaaatc
     9541 atatctgcac cgtctaaagc ttcgcgtaag gattgttcac tttcttctgc cgctttttgt
     9601 ccaacttctg gctgagaacc agcacctaaa ccacgagtat atttggggcc taattggata
     9661 actgtttcag cttttgaatt ttttaatgct tgcacgtctg tgttggctgt aatgaattca
     9721 acacctttaa cgttttcttc aatcattcgg tttacagcat ttccaccgcc accaccgact
     9781 ccgatgactt tgattactgc gccgtcattg atattattat caattgagaa ttccatatct
     9841 tattcctccc aggatgaatt aatcaaaaat atttgtaaag aaacccttga ttttattttt
     9901 tacattttct ccggactctt ttggttccgg ctcatcgtag attgtttctt caggtgcttc
     9961 atagctctca tatgtgtttg ttgtttcttg ttcaactacc atttgacgag ctgctgaggt
    10021 ttcacccgta acagcaattt ttgccaattg atacacttca ctcaagtttg ctgaataatc
    10081 cacgatactt attacatttg taaataccgg gtttctcagc cccatgtgat ttggaacata
    10141 caattttaca tttacgccga agatttcttg tgccaagtcc acaacaccag gaagactagc
    10201 agctccgcct gtcaatacga cgccacctgg caactctaat gcttcgattt ggtccaatgc
    10261 atcttttgct ttgttgaaaa tctgttccat tcgagctgaa ataatttctg acaagtagcg
    10321 ttcatcgact ttcactggtt ctgactgacc aatcacatct actggaaatt cttcatctgg
    10381 tgatgtccgt tctgggtatg catccccgta gtttattttc aacgcttctg cattgttaaa
    10441 tgacgtattt aatacaatag agatatcttt agtgacaaat tcgccacctt cttggtcaag
    10501 attggtaaac ttcaattgct tatcatgcat aacagccgtt gttgtttggc cgccacccat
    10561 atcaatgacg atcgtaccaa aatctttttc tccgtcagaa agaattgatt cagtcagcgc
    10621 taaaggagta atcaccattt cgttgacgat taatccggca ttttccacgc atttacgtat
    10681 gttatggatg atcgttttag gtcctgtaaa caacagaccg tacatttcta atcttacgcc
    10741 gatcattcca cgaggatctt tgattccctc gaacccatcg acagtaaaat cttgtggcaa
    10801 tattgacacg atttgacgtt caggaggtat tgaacgaaca agggcagctg aagccacatt
    10861 tctgacatct tcatcggtga tttcctttga atctccgtta actgcaatca ttccctggca
    10921 attttctact tcaagcatgt ttgctggcaa accgacactt acgcctttga tctggatacc
    10981 tgctttttct tctgcttgtc gtactgctcg ttgtatcgct tggaccgttt tatcgatatc
    11041 aacgataatg cctcggttaa tcccttctga ttttgcgttt cctactccaa taatattcat
    11101 ttggctgtcg atatattcag ccacgacaac tttaacagat gtcgttccaa tatcaaggcc
    11161 tacatacatt cctgtttttg ccatgaatgg gattccctcc tacttactat tccatataca
    11221 agcgaacaac tagcgtccgt tttaaaatat acttattaat agatatattc actgcttcca
    11281 attctaccat aaattactgg tttttagaaa gtacattctt actttttaaa gttgttttat
    11341 caattttcat ttaaacttga cggagttgta cttggattat cttctatttg acttgtttca
    11401 cttgaatcat ttgaatcaga tgatccgttt gtgctagacg atccaggtgt ttgtgctttt
    11461 tgcgtttctg cctcttcact tgattcgttt gtttgatccg cattttcttc actttcagtt
    11521 gtttcttccg attggcccga ttcttccgtt tcttgagtgc taccgtaagg atgcgagaag
    11581 ataccaactt ccatgtcgat cacacctttt tcatccatat ctttggctac ttgcgaataa
    11641 tatttcattt ggttcgcaag attattgata tttacaataa cttgattgcc gtcattcata
    11701 ttcaattgga tcagattttt attgcttgct cttggcgtgt acttgatttc agaaattgct
    11761 ttttgtaatt ctgatgataa ctgattgtat tgctttgtta attctttgat ttttgcagag
    11821 tctttgaact cttccaagat agggagattt tttgtaggat ttgctacttt ttcatctaat
    11881 actgttccat tttcaatcac tgggtagtat tgattgtttt ttgcgatcaa agcaatctct
    11941 ttatattctg ttacatccaa atcgaattca ttcatccctt tgaaatgtat atttgctgtt
    12001 tcgattcttg gctgtgcttt tttaaggcga tcgatataag tagatcgatg aaaatactgt
    12061 ccccaaacat tttctcctaa tgacaatttt gtatcagaga tgatttcttg gctgttgact
    12121 gtcttattgc ctgatactgc aactgcttgt aatttgctat aaggagaaac ataatagatc
    12181 aaaaaaatca gcggtatacc caatacagtg atgatcaaag ctaaccgacg aaatagtact
    12241 ttgtgccgtt gactttttaa attgggcagc cggttcaaaa aagaaccgtt atatggtcca
    12301 ccgtttttct tttgcttgat ctcttcaaaa aattcgattt ttttctcgga ctggctttct
    12361 tcttgctgtt cctcttgaac cgttttttct tcttcagaat ctgaaagggg tgtatctaat
    12421 gttatgtctt ctttttttgg ctcttggttt ttcatctctt gttctgccag atattttcgg
    12481 ttagcctttt gccaaggagt caaattgttt tcatcagaag acggatgaga caattttttt
    12541 ggttcttttt tcttactcac taagaccacc gcctttattt tttgattatt tgttttacta
    12601 agtcataaag acgttttgaa gcatcaggga ttccttgttc tttagaagct cggcacattt
    12661 gcttttgcaa ttcttcatca cccatgattt cgttgactgt ctgacttagt gattcacctg
    12721 ttagttcatt atctgcaatc atttttgctg ctcctgcgtg aacaagactc attgcatttt
    12781 tagtctgatg atcattcgta acatatgggc tgggaaccaa cactgccggt aaccccagag
    12841 ctgtgaattc tgcgatcgaa gttgcaccag cacgccccac cagcagatca ctggaagcca
    12901 tgacctctgc catatttttg atataaggct ggacactaat attttgtatt tcaggattta
    12961 ttttatttag gatctcttca taatatctat ctcctgaagc atacaaaact tggtaagaag
    13021 aaggaatata ttcacttaaa aaaactgata ctgcttgatt gattttaagt gctccttgac
    13081 taccgccaaa aatcaagact gttttctttt ctggatccaa atcaaatgtt ttcaaaatat
    13141 ctgatttttt agtatctgct acttcttgtg ctcgaggatt acctacaaga actgctttgt
    13201 tttcaggaaa aaaggaagcc gcatctttaa atgaaagagc gatccgatcc acatatcggc
    13261 ttaaaaattt attggtaaca cctggtacac tgttttgttc atggataact gttggaatac
    13321 ccattttggc tgcagcgtac actaccgctc cggaaacata tcctccagta ccgataacga
    13381 tatctggttg aaaatcactg aggatttttt ttgcttgacg gatactctta agaaacaatc
    13441 ggaccgtctt gatgttatgc atggaaattt ttcgtttaaa tccttgaatc tccaaggtat
    13501 ggaaaggcat tcctgtatca gggacgattt tgttttctag acctcgtttt gctcccacat
    13561 acatgaattc agcattcggt tcttgagtct ttacataatt gacaaaggca agagctggat
    13621 aaatatgtcc ccctgttcct ccgcctgtaa ccaaaatttt cattttttat cgctcgcttc
    13681 tactttttct attttaatcg gttcacagct tccatgaatc tgtttcctct agtttcaaaa
    13741 tttgggtatt gatcccagct ggcattcgct ggtgataata aaactacatc accatctgta
    13801 ctgttttcta atgctaaagg aactgcttgt tctacagaat cggctgttac tattgtctcg
    13861 atgcctgctt tttttccagc atccatcaac ttttcttttg tttggccaaa tacgaccatt
    13921 gctttgattc cttttaatga aggaattaat tcatcaaatg tatttcctct gtctaatccg
    13981 ccagccaata acacgacctt agacgtttca aatccactta gagccatttt agtcgctaaa
    14041 atattggttg cttttgaatc attataaaat tttctgcctt ggatttctcc tacatattgc
    14101 gtacgatgtg gtacgccatg gaaaaaatgc aacgtttctc gtatagcttc atttgatata
    14161 ccatacaatt ttgctacaga aatagcagct agtgcatttt ctacattatg cttacctggt
    14221 acgccaagtt cagatatttc cattattttt tcttttttat agtatattga gtaatcgtca
    14281 gcatacacac cgtcttccaa cacttctttt gtcgagaaag gcagtacgcg tgcttttgtt
    14341 ctttgcgcta attcttgtag ttctgactga ttccagttaa ggatcagata atctttttct
    14401 gtcatgtttt tttgaaggtt ccatttagct ttgacatatt cttttcttgt accatgataa
    14461 tcaatatgtg cttcatagat attggtaatc accgctatat gagggcggaa atctgtgatt
    14521 cccatgagtt ggaagctaga caattccatc acaattttat cttctgattt tgcttcttgc
    14581 gctacaccac ttgctggata cccaatatta cccgctaaac gtgcaattcc ttgatctgct
    14641 cctgcattca gtagtagtcc tgtcatcgtt gttgtcgttg tttttccgtt cgttcctgta
    14701 atcccaataa ttgggcattc cgctacttcg taagctagct caacttccgt aattaccgga
    14761 atacccattt cttgggcttt tgctactaaa ggatgactgt agggaatccc tggatttttg
    14821 accatcaatg aaaattcttc atccaacaat tcgattggat gactacctgt tattactttt
    14881 atccctaaag aaagcagctc ttgtgcttcc ggattttcgt caaaaggttt tccatcatta
    14941 acggtgacaa gtgcgcccaa ttcatgtaat aatttggccg cacttactcc gctttttgcg
    15001 agtcctaaga caagcacttt cttattttca tatgttgata tttttttcat ttcccgtcat
    15061 ctcctaccaa acgatccata atgtgatcaa cgaagtcaca atgctgatca accaaaaaac
    15121 tgtatcaatc ttccactccg accatccgca catttcaaag tgatgatgaa ttggagacat
    15181 tttaaaaatt cgttttcctg taagcttaaa ggaagtaact tgaagcatca cacttgctgt
    15241 ttccataaca tagatcaaac caatcaaaag caatgtccat tcttggttta gcattataga
    15301 aattgccgct aaaagtccgc ccaacgctaa tgaaccgaca tctcccataa aaatcttggc
    15361 aggttttctg ttgtagacga agaaacccag taaacctcct aaaacactta agcaaatgat
    15421 taatacatca tactgatgct gatgccaagc aattagaccg taagctgcaa aagagatact
    15481 tcctaagcca gcaactaatc catcgatgcc atctgtcaga tttactgcat ttgaaaatcc
    15541 aacaagccag aagagagcga aaataccata aatgattcct aaaggtagat ctagtccgaa
    15601 gaaattcaat acacctggat aaccttcaga acggtaaaca agataaaaga tgattccccc
    15661 tagaatctgt ccaatcaatt tctgcttcga gttcagtccc atgtttcttt tcttgaatat
    15721 ttttataaaa tcatcaagaa agccgattac tccgtataac gctaatacaa aaagcaagat
    15781 gaaaagagta ggagttagct gtttttgcca agctccaacc caaattcctg tcaaaataga
    15841 gccgatcaga aacactaagc cgcccattgt aggagttccg gctttgctat tgtgccactt
    15901 tggtccctcc tcacgaatcg cctgaccttg ctttttcatt tgaaaataac caataaatag
    15961 cggcattgcg gcaattgtca ttgcacaact gctgacaatg ggaatcaatg cttgtgtcca
    16021 ttccatgctc ttttctctcc tatttctctt cttattcttc taaagtaaaa ctaagtttgt
    16081 cttcagtgat tttttcatat ggagcaagtt cttgtttgac acaataacca tctccatcaa
    16141 aactaacttc aataccgagg attttcccca gcttcatgat atctgctttt gaccatcctg
    16201 ttacgtcagg catcagctta tcaccaccag tatataggat cagtttttct ccagagatca
    16261 actgatcacc gttggcagta gattgttttt gtactttttt accattaccg atcactacag
    16321 gttgcagtcc acttttttga gcatcagctg ctgcaacgtc cgcttcaaga ttacgataat
    16381 cggctacgga tactttttct gtttttgttt ctgtatctga atcttcttca gtttctttaa
    16441 aatccattgc ccgtttcatc aatggatttg cgattttagc caatgccatt cgatcatatg
    16501 tcttagggtg tttcattgtt agatacaaga catagtctgg atcttcagaa ggaaccatct
    16561 ccacaatcga atacaggtag gctgtatctc ctgtttgata tccccctgta tcagaagcga
    16621 tttgggctgt tccgtttttt gccgatacat tgtatccagg tacactatat acaccataag
    16681 cactaccgta gttttcgctt tcgacaacat ctctcatata ttcacggact ttttctgttg
    16741 tttcttttga aaaaggttgc cctagaactt ctgtttgagt tgttcgctct tctccagttt
    16801 ggggatccac cactttgctg atatatcgag gttgaatcat tgtaccgtta ttcgcaatag
    16861 aagtgaaagc tttcatcatt tggaaattcg tcacacctac cgcttgtcca taagcactca
    16921 ttgctcggtc gacaatattt aatgtaggta aagcaccgtt tacttcatca tctaatccag
    16981 aatgagtact ttgtccaaat cccagttttt gtaaatagtt ataccatcgt ccgcctaatc
    17041 gctgttccag tatgaccatc ccgacgttac ttgaccatga aagagcttga cgcatggtca
    17101 gcacaccttt ttcaccaaag tcatggtcat tgatcgtagc atcttctact tggatttttc
    17161 cagattggaa ggtttcattc tcgttgaatt ctccttcttc gatagcagct gcagtagtga
    17221 aaactttcat ggttgatcct ggttcataac tatcttgtac caagaaatta cgccagatag
    17281 catcttttcc agtcaatcct tccattgttt caggattgaa agtaggtcgt tgtcccattg
    17341 ccaagatttc tccggttttt gctttcatca ggactgctgt caattcttct ggctgatatt
    17401 cttcatttac ttgatccatc agtgtttcta aataactttg tagtctgctg tctaaagtcg
    17461 tgtagatatc ttgtccgtcg acagcttttt cttcttctgc cactgtacct ggcagaggat
    17521 tttgaaaatt atctttttga taaataatct taccatcttt accgctcaag atatcattgt
    17581 aagcagactc caaacctagt ttaccaacta agccattctc atctttgtct ggaactgcgt
    17641 atccaataaa atgagaagaa aagacgccat ttggatacat ccgagcctga tggtcgacaa
    17701 aataaagacc agcgacacct tcttttttca tgtccgcttc gatgttttgc ttggtttctt
    17761 gggagatatt tttcccatag ctaccaaatt caacttgata cagctctttt tttgcgccgt
    17821 cctctaaaat attcagtgca tcttttttgt ctatagaaag attcttatgc aggatttctg
    17881 ctattttatc aaaattcttt tcttcaacat acagtttttt atccccagag gtatatgttt
    17941 ttgagagtat cgctttaatt gaataagagg aggcatcttc tgcgagcgcc acaccgttac
    18001 gatcataaat cgttcctctt tttgctttta ctacttcact tccttggtac aactgttttg
    18061 ttttttcagc taatgaggta ccagcaacat gacctccaat aacgatatac gagaaacgga
    18121 cggcaaataa aaagaacaat ccaatactcg tagcaaataa aatgatacct acttttttgc
    18181 gattgttcat cggatttaga tttttctttt ccataaaccg ccgaatttta tttttaagac
    18241 tcatttactt cactttcctt aaattgtcgt cattaataga caatcctttt tcttctgcga
    18301 tttgtttaat ccgttcagta cgagaaagtt cgtttttttc ttgttctaaa ctcgtttttt
    18361 cttgcttttt atcattgatt tctgtttgta ttgacgtgat ttctttttca gctccgctga
    18421 tatttgtgcg cagcataact gttaaaacac ataaagcaac aatagcaatc gccacgcttg
    18481 ccacagcaat tttttccaaa gcagaaatct gctttaatcg ttttgccggt gattgtggaa
    18541 gaatgatttc ttgattcgaa gaagaatcag gcatttgttg ttccggctga tccaccaatt
    18601 caggtaaatc atattcgtaa tcttgcattt tctttaattc tgccatcatt ctactcctct
    18661 ttcattctta ttttttctgc aatacgtaac tttgcactgc gtgatcgatt gttttctgtt
    18721 agctcttcct ctgaaggaag gattggttta cgagtaatga ctttcagttc tggttggaat
    18781 tcgtcaggta tgacaggaag tcccggtggt agatcttgta ccgtactata ttctttaaac
    18841 atacttttga caattctgtc ttccaatgaa tggaatgtga ttacgctgat tcttccattg
    18901 actttcaata accggattgc ctgttctaaa gaggcctctt cggcaccgag ttcgtcattt
    18961 actgcaatcc gcacagcttg aaaaattctt tttgcaggat gcccgccttt tcttcttgcg
    19021 ggagctggga tgacttgttt aatgatttcg acaagctcac cagttgtttg gattggtttt
    19081 gtttttcgta ctcgttcgat ttcacgagca atttgtttgg aaaatttctc ctcgccatag
    19141 cggaagaaaa tctttactaa ttcatggtag gaatattcat taattacgtg ataagcggat
    19201 aaaggagctg actgatccat gcgcatatcc aacggtgcat cttgatgata actaaatcca
    19261 cgttctgctt catctagctg aggagaggaa acccctagat catacaaaat gccgtctacc
    19321 ttatctacat gctcactcaa gcgttcttct aattctctga agttcgcttt gataaatgtg
    19381 accatacctt gttcgacata agaagctaaa cgcagcttgg cgtgatcgat tgctttttga
    19441 tcttggtcaa acgcatacag atgcccttta gaacttagtt ggcttaataa atattcacta
    19501 tgtccggcac cgccgagtgt acagtctaca taaacgccat ccggtttgat gttcaatcca
    19561 tctaccgttt ctttcaacat gactgtatag tgttgaaatg tttcagtcat tagtcagcct
    19621 cttcctattc ttatagtccg aaatcaatca tactttcagc aatttcgtca aagttctctt
    19681 ctgctgctgc agaaaaatct tgccatcgtg tctcatccca aatttcaatt cggttagata
    19741 caccgatgac tacgcaacct tttgtcaaag ctgcataatt tcttaacgtg ctgggtatat
    19801 tgattcgtcc ttgtttatca agttcgcatt cagtggcagc cgaatagaag aatcgcacaa
    19861 aagtacgtgc gtctttttta gccaacggca tttcgttcag tttagcttcc agattttccc
    19921 attcgctcat cggatagccg aaaagacagc catccaatcc gcgtgtcaga acaaactttt
    19981 cgccaagttc ttcacgaagt tttgagggca cgattaaacg tccttttgca tcaatattat
    20041 gttggaattc acccatcaac attgaaagcc ccccattcct ccactaacta aaattagtct
    20101 accacaatcc cccactttct accacctttt ctgggaaatc cgggaacaac tttttttatt
    20161 cataaacatt tttttaaaaa tcaagctgca aaaagccttt taaaagactt tttacagctt
    20221 ttctattttt ttcataggta agaaagtatg ttcacaatga tcaagacagc gtacaagacg
    20281 attgtcaaca gaaaaaccaa acgccaagtc atcttgaaat aacgcggata aataatttct
    20341 tcataataat atgcttggaa aacagcaata caaattccca ttagcaaaac agaaataaag
    20401 aaaaacggta agatagaaat tgtccctgta tttctagaca atgcatgcat accgccccac
    20461 aaaaaaggaa tcgccaaatc aggggctttt attttaaaac gacgatttaa cgaaaataat
    20521 tttacaagaa attggcaacc aaacaaaacg attaccgggt aaatatacca aaacaagata
    20581 agtggtgtga atgtgcccat gaacgtaaac tcctttttta tcgttgcttc aagtatactg
    20641 tatatacttt acacaactgt ttcattttat ttaaatttat gcagttatat tctatatctt
    20701 acaaacacat tccatgtatt ccaattccct atccgttata ataaaagaaa caggcaacag
    20761 gaggaaaatc atgatcaaat attttacatt agataatgag aaactgcgta ataccggaaa
    20821 ggaaactgac caaacgatct ggtattgtgt agaacgtcct aatcaagaag agattcatca
    20881 gctgacacat caatttgaaa ttccgtcaga ttacatcact tccatattgg acgatgcaga
    20941 aaattcccgt acagaagaat tcaatcaatc aaagttgaca aagccagcgt tattattgtt
    21001 acaatatcct tttgctaaaa caagtcctag cggttatttt caagtggata catacccgct
    21061 ctctattatt ttgacgccca aaaagaagct tatcacaatt acgaatcatg aaccactgtt
    21121 tttaaagaaa gtctttagcc agacctttcc aaaaagtgat atttcgccta atctcaatat
    21181 cttctttcaa ttcatttggc aggtcacact ctcttacaac agttatttag ctacgcttca
    21241 atctcaatgc gatactttgg aagccaaact tcaagtatcc actgaaaaca aacaacttta
    21301 tcaaatcatg gatatccaaa aaagcctcgt ttatttcaga gaagcgactt ctgctaattt
    21361 agatgcattg aaagaactag cgcaaaataa acttttccaa gaaaatcatg ctttgaaaaa
    21421 tcatttacgt gatgtattgg tggaaacaga gcaggcaatg actacttctc gcattcaatt
    21481 aaaactagta gaacaaatga atcagacttt ttcagcaatc gtatcgaata atctgaataa
    21541 tattatgaaa attttaactt ctttaacgat tattttaact ataccgacaa tcgtcggcag
    21601 tatctatgga atgaacatta agttacctat tgctgaaagg gatgatgcct ttatatggct
    21661 agttctatta acattattta tttgtatcgt aactacctat tatttaaaaa aagggaagtt
    21721 tttataaaaa agccctcttg tggaaatgga aaaaagattg tacacttgaa tcaactaatt
    21781 aatgacgagg ttgaaaacaa tgcgtgaaaa aaaacaaaca agtacttttg ctaaagtaac
    21841 aaaatttgtc atttggacaa tgctgatttt aacgattgga tctgttgtat tgactgctgt
    21901 aatgagtgtc atgtaaaaat aaaatatagc tatcagctgc tgaaaaggac ctgaaccact
    21961 gcgaggtggc tcaggtcctt tttgcgagtt tcaatagttc ttttgcatgt tcgagtgtga
    22021 gttttgtaat ctcacttcca gccagcatac gtgcaatttc ttcttctctt tcttttgttt
    22081 tcaactctga aacagaagtt tgcgtacgtc catcaatcac tgctttcaca atataatatt
    22141 gatagtcggc gactgcagca acttgaggca gatgagtgat gcacaaaact tgagagtatt
    22201 tcgatatttg agaaatttta tctgcgatgg cctgtgcaac tcttccgctc acacctgtat
    22261 ccacttcatc aaaaataata cttgtcacac cttgctcaga agaaaaaata gttttcaaag
    22321 caagcaacat ccgagacagt tcgccaccag aagcgacttt caccaaaggc ttcagtggct
    22381 ctcctggatt ggtcgtaatg taaaattcga tttggtcaaa tccatcatga ttcaattgtc
    22441 tattttcttc ttttaagaaa cgtacctcaa actcagtgtt ttccatatac agacttttca
    22501 attcatgcag aatggatttt tccaattctt ttgcgatttt tttacgcgct tcatgaagat
    22561 ctgcggcttg ttgctgaagt agaagctctt tttgctctag ttcctctttc attttgtcca
    22621 attgaccttc tgaaaaatca gaagaagcta gctcttcggt gatttcgtca tagtaagcca
    22681 atattgactc aatggattcg ccgtatttac gtttcaactg tcggattaat tctaaacgtt
    22741 gcgtaacttc ttctaaacga ctctcatcta actccaataa atcaatctga cgactcatat
    22801 cgccgattgc atcttgcagc aaatagtaag cactttggat attttcataa attgcttcat
    22861 actccacatc tagatgggcg atcccttgca gttctgaaac ggccaagcca acaccatcta
    22921 cactgttttg ttcattattc gtcagtgctc cgtaacctgc agctaatcca tcagcaattt
    22981 tttgataatt gctaagtttt tctctttctt cttttaattt ctcttcttct ccgacttgca
    23041 aatcagcagc agctatttct tcttgctgga aatggagcat atctattctc tgaacatagg
    23101 atttttcatt ttgctgcact tttcgtaccc ttttttccag ctcacgataa gagatatatt
    23161 cttgctgata actctttttc ttctgttgga aagctacatc tccaaaacga tccaataaag
    23221 caagatgtga ttcaggctgg agcagttcct gatgttcgtt ttgtccttgg atatctacta
    23281 aataactgcc gattcttctc agattagcta atgtgataat atgaccattc actcgacaaa
    23341 cattttttcc agttaaggac atatcccgtc gaacaatcag attgtcctca tctgtttcga
    23401 tccctaattc aaccatcagt tcagagaatc cttcttgttt tggtagttca aacaaccctt
    23461 caagcacaca tttttctgct ccttgacgta tataatcgct tgaacctctt cctcctgcta
    23521 acagacctaa agcatcgata ataatggatt tacccgcacc agtctctccg gtcagcgcgg
    23581 tcattccttc gtgaaaagaa agccgcaatt ccggaataat cgcaaagtta gtaatactga
    23641 tttcttgtag catcacattc gctcctcaac ttaaaggtaa tgcaggaagt ctttcttcaa
    23701 taaaactgcc tggtcttcag ttcgtgtaat catcaaaaca ttatcgtcat cattgataat
    23761 cgaaaataat tctttcttgt aacgtttatc aatcaaattt gctgctgccg aagcatttcc
    23821 cggcaatgtt ttcaaaataa caaatttttc catttggtca actgccacga aagcgtcttt
    23881 taatagtttt tccaatttag tatttacatc ttcgtttgtt tcagctggta tgctataacg
    23941 gtagccgcca tctgaagccg gtaccttgat cagtttcagt tcctttatat ctctggaaat
    24001 cgtagcttga gtaacagata ttcctttgct tttcaggatt tcaacaaatt cttcctgctt
    24061 tcggatatct tgctcactca acaatcgagt aatcaaacga tgccgatctt tttttctcat
    24121 tacgtcttcg ctccttagca tattcttctt aatcaatgat agcttatttc cttggtataa
    24181 taaagaagaa tccgcatact tctcacttga ttttcacagt tacaatttat ttatggtcaa
    24241 actgttcgtg tgcatttgaa accacttctg ccatattaat agctgaattg attgttccgc
    24301 tttcgggtac tttcctcaag tgagccaaga actcgatatt tccttctccc cccgtgatcg
    24361 gagaataact taattccagc acatcatatc caccttgtgc cgcaaatgac aaaatgtctt
    24421 ctaaaacttt ttgatgggtc tgagggtcac gaacaattcc gtttttccct acagcttctc
    24481 tgcctgcttc gaattggggt ttgattaaag caatcacttc tccctcttct tttaaaatag
    24541 catgtaatgg aggcagcatc agttttagtg aaataaaaga aacatcgata acagcaactt
    24601 ctggaattcc taaggtaaag tcttcgggtt ttgcataacg aaaattcact ctttccatta
    24661 cgacgactct aggatcttgt cttattttcc aagctagctg attgtagcca acgtctaaag
    24721 cataactcat ttttgcgcca ttttgcaaag ctgcatcagt aaatccgcca gtggatgcac
    24781 caatatctaa caatgttttt ccttcaacag atagatcaaa tgctttgagt gctttttcta
    24841 attttaatcc ccctcgagaa acataaggaa gttttttacc tttgactctt aattcactac
    24901 tcactggaat tttttctccc ggtttatcaa accgctcgtt tttttcgttg taaatcaaac
    24961 cagccatcac tgaacgtttg gcttgttctc ttgtttcaaa taatccttgt ttgaccgcta
    25021 atacatctac gcgttctttt tccattcttt ccctcatttc tccacatcaa attgcttgat
    25081 catctggcgc atcagcccgc catcaaaaga aaaaacgtcc tcttctaatt tgtctatgat
    25141 cttgttggcg gataacagct caatctccaa cgcctcacgt gtcttttcta ggcctaataa
    25201 acgcgggtaa gtattttttt ccatacgttc atctttacca gctgttttcc ctaatgtttt
    25261 cgttgtacca atcacatcga gtagatcatc tcttatctga aaagctaaac ctaaatgttc
    25321 ggctaatcgt tgaagtagca ataagatttc ttcaggttgc ttcgctaata tgccaccagc
    25381 cagcaaggca taacgaatca aatgccctgt ctttctttca tgaataaatg ctaattcttc
    25441 taaagaaagc tttttcgatt cgccctcgat atctgcagct tgcccagcaa ccataccttg
    25501 tgatcccgca caaacagcta attgctgaag cagcagaagc ttaggagaat tacctaagtg
    25561 tgccatactg attagctgga atgctcctgt aagcaaacca tctccggcca atatagctaa
    25621 tgcttcgccg aatactttat gattggtagg ttttcctcta cgaagatcat cattgtccat
    25681 cgcaggcaaa tcatcgtgga tcaaggaata cgtatgcacc atttctaaag cagctgctac
    25741 ttgatagacg ggaacatcaa taggctcatc aaaggaagca acggccgtta aaagtaataa
    25801 gggacgtatt cttttcccgc ctgctttcac tgaataactc atcgcatcat agagtcctct
    25861 atccgttgta tgctcatcta aaaaagacaa gatttctttt tctacagcag gtagatgaat
    25921 agctgaaaag tcagttaatc tcatctcagc tttcctcgtt tccgtcaaaa tcaacttctt
    25981 catttgattc tgtcatcatt ttcgtcaagg tctcttctgc tttagctaat gtatcttggc
    26041 aatgtttgct taattccatt cctcgtttaa acgaatctag agctgattct aatggaacgt
    26101 ctccttgttc taactgcatg acaatttttt ctaattcttg cagtgactct tcaaaagttg
    26161 gattttccat atttttattc tcctttgtct atctcttgta cttgtccttt aacgattcca
    26221 tcttgataat ggatcgtcag caaatcccct tgatgaatct cggtgacact ttttactact
    26281 tgatcattta acgtcgtata gctgtagcct cttcccatga ttttcaatgg actcaacaaa
    26341 tcaagagatt gaacagcttg agcaaaacgc tgctgctgtt gtttcatata ttgttggatt
    26401 ttctcttcta agcgtttttc tagataagca gtttcttggc gagctgcttt aactcgataa
    26461 agcggagcca cttgttccaa ttggtggctt aatgcgatcg tttgtttttc tttcgcatga
    26521 taaatttgct gtgtagcttg atataatcgc tgtttcaact ggtctaattt gatggtctga
    26581 ccttcataca atctttcagg ctgccgaaat acataggaac gtcgagcacg ctcgaaccgt
    26641 tcttgtttgc gctggatctg gcgtacaaac gcctgctgca atctagcttg ccgttcttga
    26701 atgcgcatca actcttctga caaaacagga acagctaatt ctgcagctgc tgttggagtg
    26761 gcagcccgaa catctgctac taaatcagcg atcgtggtat ctgtttcatg tcctacagat
    26821 gaaatcaccg gcgtattagc ttgataaatc gcccgagcaa cccgctcttc attaaacggc
    26881 cataaatcct caatcgaacc acccccacga ccgatgatca ttgtatcgaa attgcccatc
    26941 tcttctactc tctggatatt cctgacaaca ttgtccgctg cttggtttcc ttgtacaact
    27001 gttgggaaca aaaccaattg gacaattgga tatcttcttt tcaccgtcgt gatgatatcg
    27061 cggataaccg ctccgcttgg actggtaagg accgcgattc gctttgggaa acgtggcagc
    27121 atttttttag gcccttgaaa aaggccttcc ttcatcagct tttcttttcg ttcttccaat
    27181 tcttgatata aagcacctat tccatcaggc tccatgtgct ctacatagat ttgataagaa
    27241 cctcccgctt catataatga aatacgccca acgacaagga ctttcattcc ttcttttggc
    27301 tggaatcgta atttttggaa tgctcctttg aacataatag cagatatttt tgcatggtca
    27361 tcttttaagc tgaaatattg atgggcattg gcacgtaaac ggaaattcga tatttcgcct
    27421 gttaaatata cgcgttctaa ataaggatcg gcatcaaatt ttcttttcaa atacttcgtt
    27481 aatgtagtga cagttaaata ttcttgtgcc atctattcac ctgctccaaa agcttcggca
    27541 gattttacgg tttgatacat cagcatcgtg atagtcattg gccctacacc ctttggcaca
    27601 ggagtgatcc agcctgcaat aggctccact tcatcaaatt tcacatcacc gatcagctta
    27661 ccgttagcat cacgattcat ccctacatct atcactacag cacctggttt tacgaattct
    27721 tttgtgacaa aatgtccacg tcctattgct acgactaaaa tatctgcttc ttgtgccagt
    27781 tcagccagat tttcggtttt agaatgagca acagtaacag ttgcatctgc catcaagagt
    27841 agctgagcca tcggtttacc gacgatattg cttcgtccga taactactgc tcgtttaccc
    27901 gccggatcga tagaataagc ttcaaacatt ttcatgatgc cgtatggagt acaaggaatc
    27961 ttgtccggtt tacccgcaag gagtctgcct aaattcatcg gatgaaaacc gtctacgtct
    28021 ttttcagggc ggatagctaa caggatcttt tcttcatcta tatgctttgg caatggtaat
    28081 tggaccaata taccatgaaa actaggatct tgattgtatt tttcaatttc tgcaagtagt
    28141 tcgtcttcag aaatcgtctc aggataacgc tctaccaatg aatggattcc gatttctttt
    28201 gctacacgtt ctttatttct tacatagatt tggctggctg gattttcccc taccaataaa
    28261 acgactaatc cgggatggat accattatct ttcaattttt ggattttttc agcaatctct
    28321 gcctgcattt tttcagctaa ttcttttccg ttaatcaatt ctgccatcta accactccca
    28381 gatatttttt gtgttttcat tctaccatag aattttcccg atttataaca ggtttatgat
    28441 gattcataga aaaaagctag gacttacgcc ccagcttcta attctttcaa tacattggat
    28501 aaaacaccgt tgacgaattt acgtgaacgg tcatcactaa atgtttttgc caactcgatt
    28561 gcttcattta aagcaacagc cgctggtaca tctgtcacat aagtcatttc aaaaatcgcg
    28621 atacgcaaga tcaccaaatc catttttgag atccgattga tgtgccaatt gttttttaaa
    28681 tgtttttcaa tcactttatc caattcatct ttttttgcac agacaccgcc aactagcgta
    28741 tcaagataaa caggaacaaa ctctgattca tcttcgttga tcatgtcgcg gtggtctaat
    28801 tcgattgcgt tgaaaatcgc atcttctttt gttaggtctg cattgaagtc taatggaaat
    28861 aaagcctgca atgccatttc ccgaatctcg tgtcttgata attctttatt cattctcttc
    28921 ctcttcgtct tcaaaaagtt cttcgattgt tggttgttct aatttttcag gaacaacagc
    28981 tactacatgg atattgactt cagataaatc gatatccgtc atgaaaagga cttgctggct
    29041 gacccgttcc tgcatttcca ttgctacttt aggaactgaa acaccatatt ctagataaca
    29101 gtaaagatct actttcaagc catcttcctc gtttgctaag taaacacctt ttccgtgagc
    29161 tgcacgtcct aataattcag tgacattgct ggcaaaagtt ccgcgcattc cgtatacgcc
    29221 ttctacttta gaagctgcaa tcccgataat tacttcgatg acttcaggag cgatcacgat
    29281 ctctcctaaa tcttggtttg cgtttaaaat caaatttttt tcatctgcca tttatttatt
    29341 cctccctttg cctaagcacg agatacatcc gaattctcta tgatgcggca tagtgatcac
    29401 gtggtaagaa aactgccgcc gatcagctgc aaactgctat gaattggaga aaacacccaa
    29461 ctttattatc tcttacagct taaaagttct tctatagtgt atcattaaat cgagtgattt
    29521 tctataacaa agtctgtgaa cactgatttt tacgcttact tcttctattg atgaaactat
    29581 aagatgatca attcttttgg tgaacgagtc aacacacgat ttccatcttc tgtgatcaac
    29641 aagtcatcct caatacgtac tccaccgatt cctggtaagt agatccctgg ttcatcagtg
    29701 atcacatttc ctggtacaaa ttgcttttct gcacgagaag aaacgtttgg tccttcgtgg
    29761 atctctaaac cgatcccatg tcctgtagaa tgtccaaatg cttccccgta accatgtttt
    29821 gaaatataat ctctagcaac tgcatctaat tggacaccag tcattcctgg ttttgcagca
    29881 tcaataactg ctaattgagc atctaagact atttgataaa tttcttttaa tttttcccca
    29941 ggatcaccaa tagcgaatgt acgtgtcata tcggaaacgt acccttcgta atagcaacca
    30001 aagtccaatg tgatcaagtc accttgttcg atgatttttt tgcttgctac accatgcggc
    30061 atagccgaac gcaatccgct agcaacgatc gtatcaaaag aaacacctga agcgccaaga
    30121 gaacgcatat aaaagtctag ttgatttgcg acttcaatct cagtcatgcc cggttggatc
    30181 attttcagga tgtgatcata tgccatatcc gcaatatgac atgctttttc gataatagcg
    30241 atttcttctt catctttcac ttctctgagt tcttcgatca tctgtgaaac aggtactaat
    30301 tccgcatcga tgatttcttc taaaaccgaa tattctaaaa atgagactgt tgtttcctca
    30361 aaagctaatg agtgcagttc ttctttgcgt actaagtttg ctacctcatc aaaaataggt
    30421 ccgacatttt taataatttc aaacccttga gcctgcgctg ccgcctgctc ggtataacga
    30481 aaatcagtaa taaaaaatgc tttttccaaa gtaataacag ctaatcctgt cgtacctgta
    30541 aagttcgtta agtaacgaag attgtacgga ctggtaacca aaaatgaatc caaattttct
    30601 tcttgcattt ttttgcgcaa tttttctaca cgtagcatca taatgaaact ccctctttct
    30661 ttgcacgctt ttttccatta tagcaaactt ttcttcgtac acgacagtta aataaaaatt
    30721 tccggaaaac ttctttttat tttaaataaa taaagccaaa aaggagtcac agtctcgctg
    30781 caactccttt tagtttacta ttttgtaaaa acttatgatc gttttccaaa gaaaaatgag
    30841 acaactgcta ccaaaatcac tgcgcctaaa atagatggaa taagtgccat acctgcagct
    30901 tttggtcccc attcgccgaa aatcgcttgt ccaatacttg aaccgactag tccagcaatg
    30961 atattcgtta cacagcccat cgaatttcct ttactggtga tcgcacctgc aattgctccg
    31021 atcacggctc caacaatcaa tgtccataac caactcataa tataacctcc ttctttgcta
    31081 atcaatttta gcatatggga tttaaagggg acaaataatt ggttttgcta tttcatattt
    31141 tcttctgcaa agtaaaaaaa taaagaaaac ctcctcttag aagttttctt tattttttac
    31201 ttgcgtttaa acatactacg aataccacgt ttcactacat caaagaaact caatgattgt
    31261 accattctat cttcatctac atattctctg atcgcacgaa gcactttttt aggttctttg
    31321 gaagaaaatg tgtatgtacc attttttttc gtttgaatgg catatcgagg aatccatttt
    31381 ccctttagca tgactgatgc tagtacataa tccacttctt cccatggaat ttggatatat
    31441 ttgcgtctat ctcgcgcatg gtaaaactca aaaccattat cacctatcat gatttgcccg
    31501 taatctgtca gacccgtaaa cgaagtagca tcaattacca agtctacttt tgtattgatt
    31561 gattgaacca ttttctccct ccagatattt gtttatgaac agtatactat ataaaaaaag
    31621 ctcgggacaa cgcccgagct atatttttat ggattataaa agtccgatga cgtgtccaac
    31681 aacaccgact acgaataaac caaggatgat aacgattgga gaaacctttt tcttaagcaa
    31741 ccacatacat aacaatgtta atcctaatgc agctaaacca ggaatcaatt ggtctaagtt
    31801 gtcttgtaat gttgttacct tttcaggaga aagggctaag ccattattaa cttgttcaaa
    31861 tgcactcttg attccttcac cacctgaagg caatttatcc cattcgatgt aagcaccttt
    31921 atctaatttt acagttgata caactggtaa gaatttgatt gatacccatc tttgtacaag
    31981 ggcagctaat acgaacatcc ctaaaattga agcacctttt gttacgtctt gtaacaatcc
    32041 gccagaaagg tcatcagtaa ttttagaacc agctttgtag ccaaattctt gtgtatacca
    32101 catgaatgcc caacgaataa tgttccaacc taagaagaag atgattggtc caaggatatt
    32161 tcctcccata gctaatgaag cacctagtgc acctagcatt ggacgtactg taaaccagaa
    32221 cactgggtca ccaacaccag ctaaaggacc catcatacct actttaaccc cttgaatcgc
    32281 tacatcatca acaggcgcgc cattcgcacg ttcttcttcc agagccaatg taacccctaa
    32341 aattggagaa gcaatgtatg ggtgtgtatt aaagaattcc aagtgacgtt ttaatgctgc
    32401 agaacgttct tcttttgttt tgtataattt tttgattgct ggaatcatag aaaatgccca
    32461 gccaccgttt tgcatacgtt cgtagttcca tgaaccttga atgaaggtag agcgccacgc
    32521 aacagctaaa cgatcttttt ttgataattt gattttttct tctgccattt tattttcctc
    32581 cttcaagatt aatagtcatt caaaatgtcg cctaaagggt caccagagtt gcttccgcct
    32641 ccattatttg atgaaccacc cattttagaa aggttcaagt aaataagagc taatgcaaca
    32701 cctaaagcac ctagagcaat caatgttaat tgagaaattg cagctacaac gaaaccaata
    32761 acgaagaatg gccatacttc ttttgtagcc atcatgttga ttaccaaagc gtaacctaca
    32821 gctacgacca ttcccccacc gatagccata ccatctgtta accatgctgg cattgattca
    32881 aggaatgatt gcacagtttg tgctgggata aatagcaacg ctgctgctgg gaccgcgata
    32941 cgaacccctt gtaaacatac agcaatgatg tgccacattt caaccttacg gatgtttcct
    33001 tcttcagcag ccgcatccat caaatgaacg attggaacag cgattgtacg tacgatcatt
    33061 gttaagaaca accctgcaac agccaaagga acggcaatcg cgatcgcaga aggaacacct
    33121 ttaacacctt gtccacctaa aactaaaata attgctgatg ctactgatgc taatgcagca
    33181 tctggtgcta cagcggcacc gatatttgcc caacctaaag cgatcatctg aagcgtaccg
    33241 ccaagtacga taccagcttc gaggttaccg gtaactaaac cgatcaatgt acatgccact
    33301 agtggttgat gaaattgaaa ttcatcaaga ataccttcca taccagctag aaaggcaact
    33361 aatacgacta aaattattga aataatagac atgataagcc tcctataaat tttaatatta
    33421 tttattacgc ttttgctaat tcagcttttg cttttttcaa aatatcatcc atgttatcgc
    33481 gtgaatcatt aggtacttta cgtacatcga atttcacacc tttttgttca agcttctcaa
    33541 acgcttcaac gtcttcttgt cccatagaca atactttact tacaacgact ttaccgaccg
    33601 aatgcgccat tgacccaaca tttaattctt tgatgtctac gccaccttca acagctgtca
    33661 atacgtcttc aggattttca aataaaagca atgctttggt atttccaaaa cgagggtctt
    33721 ttgctacttc gatcattttg ctaataggaa taacatttgc ttttacacct ggaggagcag
    33781 cttgttcgat tagtctctta cgcagatcat cctttgatac tgcatctgaa acaacgataa
    33841 tacggtttgg ttgcactgct tttgtccaag ctgtcgcaac ttgtccatgt aacagacgtg
    33901 agtcaacacg tgctaataca tatttgattt ttccgtctcc gaccacagtt ccttctggaa
    33961 gtgctccttt aggttgtgcg tcttcaactg ctgctttcgg tgcttctgcc ggttctaatt
    34021 cttccggttt tacttttaca ccgtcttttg ctgtttcaat gatgtgtgct gcaatttcat
    34081 gagcagattc cattgaaaag cgtgatgcgt aggcttcaat caacattggt aaattcaaac
    34141 cgctgacgat tgcccattta tctttgtgct cttcgaataa tgtgtttgat tgattgaacg
    34201 gtgttccgcc ccataaatca actaagaata acacttcgtc ttgattatca aaagaagcaa
    34261 ccgcttcttc caatttggct ctcaagtcat ctggaccttc acttggtttc aaaatcacgg
    34321 ctttgacgtt ttcttgttcg ccaaagatca ttgaaccgga ttgtaagatt ccttcagcaa
    34381 attgtccatg acttgctagg ataatcccta ccatatttat acctcctatt tacttttgtt
    34441 gtaacaaatt caaaaatatt aataattgct aaccattaca acagaattat ttgacctttt
    34501 ccaacaacca ttcttgctac acctttttga ttttcgctag tagttccatc aaaaggattt
    34561 tcctgtcagc aggtactttt cgaatctcta actccacttc ttgactgttc aagtaacgaa
    34621 aggaatcaat atcttgttga tttactgaaa caaaattggt aatcattgat ttgccttcag
    34681 taaaactcat accgccgata ttcaacgact tcaactgtac accgcagcgg acaaccgctt
    34741 caacatcttg gggattggtg aataaaagca tcacttttgc tcgtgataat aatccactat
    34801 gatagatttg aatcattttc tgcacggtta ttacatttgc tgtgatcccc ggcggtgcag
    34861 cttcttttag tagaaacttt ctcaatttat cttgagcgac tacatcgctg atcacaatga
    34921 tacgttcaac acctgtttgc ttcgaccaaa ctgttgcgac ttgcccgtgg attaaacgat
    34981 catctatacg tgccaatcga atatccatct gcaaaactcc ttcttcctca ctacttcaca
    35041 gaaaccccta tcatttctgt gtgaacaacc acctcctagc acacactact ataagcaaaa
    35101 agcgtgccaa cttttttacg atacactaat tgaatttaaa ggattcttaa ccaatacaca
    35161 aaaacgatac actataaaag tgtatcgttt ttgtgtattc atggcacagt gtattctttt
    35221 ttatctaact gattatggac tagacgtagc agataataca tttcttctat cggcactttt
    35281 acttttatct gatcttccaa cagaacaact gaggctgcca attggctata aagaggatca
    35341 ttcgttgtat ttgtcaattc gttttcttcc acagtcaatg gttcattccg aataatacgt
    35401 tcgatcattc ctgctgtgtg catcactagg ttgattttga atccatattc ttcatcgccg
    35461 attcccgtcg tctgagagat ttgtgtagaa aattgccaca ttggatcgat taacttacta
    35521 ccattaataa agatgaagtt atcagagata aactcaacac acgtattttt agctgactct
    35581 tcatctaggg agatgaattc agtttcctcc aattctgatt ccatcagcaa ctgatccaaa
    35641 atgacttcga tattttgatc aataaaacgt tctaaaggaa taaatggggc ttggatattc
    35701 aaatcagtaa tcccagtagt agctatgatc tgatattctt tttggataac tggcagttct
    35761 tctttcactt ccacaatcga caaagctaga acttctaatt ctgtttcttg acgcttgttt
    35821 accgctcgtt cgatcaattc tttgattctt tgtgctgtac cttcgccaga tgcgcagata
    35881 gccaaaacag cttttttctt atgaataggt tttgcttcct gaacagattc tacagaagat
    35941 atttctgcgt aacctcgaaa atttttcaat gattcatgca acatatctag atctgtccct
    36001 aatactgaga cctttcgtac agtttccaaa actagagaag ttgttaccat atcaacagta
    36061 cgtactggaa ttccagtgtc tctttgaatt tgcgaattaa acgacgcaag ggaccccata
    36121 tctactaaga gtatgacccc gcttccttca tctacttctt gtacattgcg ttcaattcgg
    36181 tccaatgctg ttttcggatc catatccaaa ggcatgtcta ctgctcgtac gttctctgca
    36241 tctaacaact gttggacaac ttggaccata ctgcttgctg tgctgtttcc gtgagctgca
    36301 acgactacac cgattctgcc gcttgcttga tttgcccgta acgagacgag tagaacggtc
    36361 aagtaatagt cttcactttc tggaatctcc acttgaaaat acgtttcgat ataactacgc
    36421 acttctttcg caacagcata ttcatctgga aaatcaaaag ccatctcacg gatgttatca
    36481 ttcgtatgcc gttcttctcc taagttgatt tttttcaaaa aagaactgat atgcaagctc
    36541 atagcataaa taaagttttg ctgaaactca tatggtaaag atcttttgac cattacataa
    36601 atctgattgg tcacttctat gattttcgga tctacaaatt ctgctaattt gctgtctgca
    36661 ttaaaagaaa aaccatggtc tttatagaaa gacttcagat ggatgttgat atctgtagaa
    36721 atgaaatgat tgattgcttc ttgatccaaa ccatccgact tcagtaaagc tgctttatct
    36781 ccgatgatat catatagatt ataaggtaac tcataggagt ccgattggat tttcatgatc
    36841 gtatcatttg gtgctacagt tatctttggc tccaatattt tagaaagctc tgccattgca
    36901 ctgcggttgc ttgctaattg aatcaatccg ctacgaatcc cttctgtcaa atcatcgatt
    36961 gtgatcgaga tttcgggtga ttgcatatga tttagaaatc ctcgtgcaca tactaactgg
    37021 atatttgact tcaattgtcc tatatttcca taggtcacac tgccgatcaa tgcttttgct
    37081 acatcttctg tcaaagaaat ctttcgctgc gtgcggtttg cttcgtgggc aatcatgatt
    37141 tttactaaat cgactttctc atttgcagga cgtttttcaa aagccggaag tttgatattg
    37201 attgggatac gccgaacaaa agtttctaat agagaagaac ctgggtcttc tgtggtcgca
    37261 cctacgatcc gtacatttgc ttcatgggat ttcgttgttt ccccaagacg actgtaagtt
    37321 ccatgatcca taaagtaaaa gatcatttct tgaccttcag gcggcaaccg gtgaatctca
    37381 tccaaaaaca gcataccgcc atccgcttga tcgataatcc ccattttttc ttcatctgcg
    37441 cctgtaaaag caccttttgc gtacccaaat aaatgactca tcaaaagctc agggttattg
    37501 gcataatcag cacaattgaa aacaatcagt tccgtttcct catctattac atgatgcgct
    37561 tttgcaaaat gatacatcgc gtgtgcaaaa taagtctttc ctgatccggt aggtcctgta
    37621 atcaaacaat ttaatcctct tggcggatac aaaatcgcag cttttgcctg ttccactgaa
    37681 tttttcatgc tgccgttagc tccaatgatt tttgcaaaga tatccttagt atctatttgg
    37741 agtgtatttt tttcttcaga gaccggcgtt tctttgtaac tgactacatg ttttgtaact
    37801 ggctttacgt gcgtaacgag tgtttgatat atgtaacgta ctggtcgtcc gtctgtcttt
    37861 atcagttttc cttcgcgcac tagctgattc agatctttac tgctatttgt tcgctgaatc
    37921 cctaacactt cagcaatttc ctgtgttgtt actccttgat catattctgc ctttgttaaa
    37981 tgagcggttt tttctttgac atacatataa attcggtcaa tgcgttttgc caatggtatc
    38041 gctccttttc tatagactat tgtacttagg cttcaaaaca taatactatg tatttatttc
    38101 tgtttcgtca actatttacc tgattttgtt ttgttgataa cacgcggata gatagaaatg
    38161 ttcaatttaa aagaacgaaa aatcccgata cgatttttat aatggaaaca agaaaaagaa
    38221 cactgtttac catataatta tttcactgat agcaaacaat atagcttcca tcaattgaaa
    38281 acgatagtta tcaaaacatc ctgattccaa attggcaaaa aaatattaag tgtaaaaaga
    38341 caaagtaaac ttcgtagcct ccactacaac ttttattttt ataaacaaaa aaggctgtga
    38401 caaaagtctt gtcacaacct ctttaatcat aagattcaat taagcttctt gagctactgg
    38461 ataaacagac acttgttttt tgttacggcc tttacgttcg aaacgtacta cgccatcaac
    38521 ttttgcaaat aaagtgtcgt ctccaccgat acccacgttt acacctggat agatttttgt
    38581 accgcgttga cggtaaagaa ttgatccacc tgtaactgtt tgtccatcag cacgtttagc
    38641 acctagacgt tttgattctg agtcacggcc gttagatgta gaaccgccac cttttttgtg
    38701 ggcaaataat tgtagattca tagataacaa catggtctgc acctcctatt tatcattaat
    38761 tgttttaatt tggatatatt ctgagttttc agcttctatc gattgtaatc ctaataaaag
    38821 attttctaat agaatctcag caatattgtt ttgttcttgt gtcattcctg aagtgacttc
    38881 ggtataaaga taaccgcctt cttcttcatt catttcaaca atcggctcaa aaccggctaa
    38941 tgaggcaatc ccgttgacag tgctgatcgc taacgcagaa acacccgcac aaacgatatc
    39001 acttccataa ggaccagcat cagcatgccc ggtcaaagtg aatgaaacga tctgacctgc
    39061 gtcatttcgc ttaaatgttc ctttgatcat atataggcct tctttctcaa acgctattaa
    39121 gcgttgatcg cgtcgataac cacttttgta tatggttgac gatgaccttg tttacggtgt
    39181 gaatgttttt taggtttgta tttgaaagta acgactttct tttgtttgcc gtgtttttca
    39241 acagtacctt caacagttgc acctgcaaca gttggagcgc ctacttttgt agattctccg
    39301 cctactaaga taacttcgtc aaaaacaact ttttctcctg cttcaacgtc aagtttttca
    39361 acatagattg cttgtcccac ttcaactttt acttgtttac caccagtttt aataattgcg
    39421 tacatgctat aaagcacctc cttctgatat acttagactc gccatccgaa gtgacagcat
    39481 atgctgtgct tattacttgt tctgagcggt tgtagctgtg gagtccacaa ttacaacagt
    39541 actagcttac cagaacagca accgttagtc aactaaattt aaaatcaaaa agtaaaacaa
    39601 aaattcaagc aatacttctg cttttctatc ctcacagttc taccaaataa aatagtcgca
    39661 ttgtgtttct tttattctta taataaaaga aaaaggagga gcataaatgg aatacccaaa
    39721 agaactgata caagaagctt ctttaagaat gaacggccgt cctgtagaag gatttatcgc
    39781 tgggcaagga cctcttcatc ctaaactgat gctcgtggga gaagccccag ggaaaacaga
    39841 gattgatacg catgttcctt ttagcggtca agctggaaaa gaattgatgc aggcactttc
    39901 ttcaactggc ttaacacgag aagaggtgta tatcaccagt gctgtaagga gccgcccgta
    39961 tcgcgtgact catcggatca ataaacggac acaacagtca gaaactgttt atccaaatcg
    40021 gacacctact cgttccgaag tatttgcgca tgcaccaata ttggattacg aactgatgaa
    40081 ggtccagcca aaattaattg cgacacttgg aaacattggt cttcaacgtt tacttggcaa
    40141 agaatatacg gttaccaaaa atcatggtca actctataca gggcctgtct tacaattaac
    40201 agaacaaaag gatgcctacg aatggtcaga aaaaaactat cgaattcttc cactttttca
    40261 tccagcagct attttctaca atcgctccct tgtacctgtt atccatcaag actggcaagt
    40321 actagcttca cttttacagc aatattaaaa agaacagaca agaccataaa atcttcagtt
    40381 tacagtcttg tctgttcttt ttcattgttt taatggctaa ttgcagtata tttcgcatgt
    40441 aaagctgtca acgcatgagc aactgtttga tgttgatgcg tgaatgtatg ttcccacaag
    40501 tgagaaagtt gtttttctac ttctggctgc aaggaagtat agataatcgt ttgtcctctt
    40561 tgctctgctt tttcttgaaa ttcatcaaaa actgcacgtg ccgtcaaatc aatacttggt
    40621 acgcctcgca tggagaaaat gacaccatcg tgatgattta gttcttccaa tttatttttg
    40681 agtttttctg aagacataaa aaacagtgga ccactaatat acactactgt ccagttattc
    40741 agcttttccg tttctggcag ccccattctt tgccaatcga tcgtttctaa tgaaatcgtg
    40801 atagcagcgc ttttgacaac aaaaaataca cagccgccga tcaccccgat cacgatagca
    40861 atacttaaat caaagataac tgtacaaatc atcgtaagga taaacaatag aactgctgac
    40921 caatatttat tagtaaatag ctccttgatc gtttcccatt cattcatacg ccaagccgtc
    40981 acaatcaaaa cacctgcaag tgctggcatt ggaatctgtg acatcactgg cgcaaaaaca
    41041 agcatggaga gcagtaagac aactgcatgt atcataccag ctaatctagt ttgtgcacca
    41101 gatttgatgg ccacgcttgt acgggcgatg gccgcagtag caggaatccc gccaaagaaa
    41161 ggaagcaaca gatttccaat tccttgcgcc attaattctt gattgctatc taagggctta
    41221 tttgccattc ggccagccga agcaccacat agcaagcttt caatcattcc taataaagca
    41281 atgctaatag ctggaactgc cacttgttgc aaggcagaaa agctaaaatc tcccatattc
    41341 aaacgattac tactgatcaa tgtttgtgga atcttaccca cagttgctac tggtaaatgg
    41401 aacaatagca taactgctgt tgccaaaatg atagccagca gcgaactagg cattcgtttc
    41461 gcccattttt ttggatagat aaacatccct aaaatgacta gccctcccat gatcaaagag
    41521 gctgtgttta tcggaaatcc tatcgtttga taactactta gtttttcaat aacgtttgta
    41581 ccttccgagt gaacaccaaa taaattatcg atctgaccta atgcaataat gatagcaata
    41641 cctgaagtaa accccgtaat tacgggtgct gggataaaag aagtcagtgt tcctaatcgg
    41701 aaaagtcctg ctgcaagtaa aaatagaccc gctaaaaaag tagcaagcaa tacaccttgc
    41761 attccctttg ttgccgcaat agacatcaag atagctgcca tcgcacctgt cggacctgat
    41821 atttgataaa aaccgcctga taaaccgcca ataacgatac cggcaatgat agccgtaatc
    41881 aaacctgcgg ccgcatcagc tccgcttgat accccaaaag ccaaggctaa aggcaatgct
    41941 acagcagcta ctgtgatccc agccaaaaga tctttctgaa atcttgaaag atcataacct
    42001 gaaaattctt tttttagtga ggaaacataa tttttgaaca cctatctaac tcctttttca
    42061 tgaaattttc taaaatacca ttcattagaa tatcatattt catgagataa agtaatattt
    42121 ttcacaaagt ttcctgttag ataaattctt attttttcta tatttataag aaaatagaaa
    42181 gcaatgccaa cagagctagc ccaccttgtt tgagtaaaat ccaacgatcg ctagtcaaac
    42241 tgccatataa tgcaacaaaa aaaatagata ttaatagcat acttgtgatt tctttagatg
    42301 cggaagaaag ataagctcca taaagtagtc ccactcctaa tagtccattg tacactcctt
    42361 ggttcttaaa caatgtttga acagattttc tgcttaactc ttctttactc attttaaaaa
    42421 ccctacttgt cgtgtcagaa tcagttctca ccgtttcgat atacatgata taaaaaaatt
    42481 caattgcaac tagagtcact aaaattctag atagtatcgt catatcttct tcctcttttc
    42541 tgatgagttt tatttttttg ctccttataa ttgtactcga taaggatgtt tttttatttt
    42601 atttgccttt gaaaacaaac aatattggca caaaaaacaa agaaaaaccg ttagaaacct
    42661 cagtttctaa cggtttttta gcgtcacagg agggattcga acccccgacc gtccgcttag
    42721 aaggcggatg ctctatccag ctgagctact atgacaagaa tcagcggaac atcaataatt
    42781 atagataatt aaattgcttc tgtcaatacg aaatagttat ttaattagca aataatgaat
    42841 tattttggcc caactactaa atcaatcttt taaaacgaga cattctcctt cagctgttct
    42901 aaggttatca attgaaaaca ggagctacat tgacttcatg ctactcctgt ttatttagat
    42961 ttcctgtatc ctttaattga cttacaaata tagaaacttt tacatcaagt tcgaattgag
    43021 ccctcgattc aatcgattat ctacaggctg ccataatcat gtatcacaac tcgattgttt
    43081 ctgattcctt tgtcattaag atcacaccat cacctaatgg caagattgtc gatgtcaaat
    43141 cgggatgatc catcacaaca cgaagaaatt catttaattt acgatggata gctcgtttgc
    43201 cacggggaat ctcttcatct gggagcaaga tcgttcctcc ttggaagatg tcatctacca
    43261 tcaaaacgcc accttttctt aacaaacgta agcattcagg taaaaattca atatattttg
    43321 attttgcact atccataaaa ataaagtcat acggaccaga caactccggc aagatatctg
    43381 ctgcttgacc ttcaagcaaa gtcactttat ctgtcagtcc tagacgttca tagttttttt
    43441 tagctttctc gatcatgaca tcgaaacgat caattgtcgt aacatgagcg ttttctccga
    43501 ttgtcgtggc catgacactt gatgaaaaac cgatagctgc tccgatctcc aagattcgtt
    43561 caggctgaag ctgtccaagt aaaaatttca aaaatacaac tgtttcatgg ggaataactg
    43621 gtacttctgc tacatgagct tcttcttcca gtttgcctag ctcacccggt aatttttttt
    43681 gctgggtacg cataaaatcc aatagttctt ctttgactac aggacgatgc atcatttcat
    43741 tgcgcatact actcctcctc ttcacacatt atacgaacaa ctgactaaat ctacaagctg
    43801 catgagtgtt ttctcgtgtt tcatgttcag aagagtcgtt ggatctctgg aatacattta
    43861 tgtttttaac attcttacaa aaatttctgt gttcgtctcc gcttcttttt caactctaca
    43921 aaggtattcc atgctgtgat tcctgttagg caaaaagcaa tcgtaaagaa taacccctga
    43981 atccatgcaa atgccgtttt tgaaaggact agtccaatat atggaatctg aaagatggga
    44041 ctaccgataa tatttctctt ttttactact ccaggctcaa tataagtctt tgtatcagct
    44101 tttgtataaa aatcttcgtg gttgttttca acggctatta cgcgacgcgt cgagattttt
    44161 tttcctgaat tttcataata tgtaataata tctccgacta aaatctcatt tgtatttttc
    44221 ggtcgtacat atatcagatt tccttgtgca taatatggac tcatttcttt gtcctcaata
    44281 atataaggag tcaaaccaaa aattcgggga accaagacga cgagaagaat aagaattaaa
    44341 aatataatca acgttataga acttataaaa gttatttttt tgtaatcagg tatcgttttc
    44401 a
//
LOCUS       DARBER010000011        41165 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARBER010000011 DARBER010000000
VERSION     DARBER010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.011"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(450..1232)
                     /locus_tag="SCK06_000638"
     CDS             complement(450..1232)
                     /locus_tag="SCK06_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150665.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1297..2133)
                     /locus_tag="SCK06_000639"
     CDS             complement(1297..2133)
                     /locus_tag="SCK06_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1150666.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2151..3560)
                     /locus_tag="SCK06_000640"
     CDS             complement(2151..3560)
                     /locus_tag="SCK06_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1150667.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3574..5397)
                     /locus_tag="SCK06_000641"
     CDS             complement(3574..5397)
                     /locus_tag="SCK06_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1150668.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6240..6566)
                     /locus_tag="SCK06_000642"
     CDS             complement(6240..6566)
                     /locus_tag="SCK06_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150669.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6612..6974)
                     /locus_tag="SCK06_000643"
     CDS             complement(6612..6974)
                     /locus_tag="SCK06_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG1150670.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7011..7226)
                     /locus_tag="SCK06_000644"
     CDS             complement(7011..7226)
                     /locus_tag="SCK06_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1150671.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7353..7688
                     /locus_tag="SCK06_000645"
     CDS             7353..7688
                     /locus_tag="SCK06_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1150672.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7764..9761)
                     /locus_tag="SCK06_000646"
     CDS             complement(7764..9761)
                     /locus_tag="SCK06_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150673.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9859..10134)
                     /locus_tag="SCK06_000647"
     CDS             complement(9859..10134)
                     /locus_tag="SCK06_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150674.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10366..11100
                     /locus_tag="SCK06_000648"
     CDS             10366..11100
                     /locus_tag="SCK06_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1150675.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11139..12281
                     /locus_tag="SCK06_000649"
     CDS             11139..12281
                     /locus_tag="SCK06_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1150676.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12376..13767)
                     /gene="sufB"
                     /locus_tag="SCK06_000650"
     CDS             complement(12376..13767)
                     /gene="sufB"
                     /locus_tag="SCK06_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG1150677.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13772..14242)
                     /locus_tag="SCK06_000651"
     CDS             complement(13772..14242)
                     /locus_tag="SCK06_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG1150678.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14229..15464)
                     /locus_tag="SCK06_000652"
     CDS             complement(14229..15464)
                     /locus_tag="SCK06_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1150679.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15461..16747)
                     /gene="sufD"
                     /locus_tag="SCK06_000653"
     CDS             complement(15461..16747)
                     /gene="sufD"
                     /locus_tag="SCK06_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG1150680.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16760..17533)
                     /gene="sufC"
                     /locus_tag="SCK06_000654"
     CDS             complement(16760..17533)
                     /gene="sufC"
                     /locus_tag="SCK06_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG1150681.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17730..18578)
                     /locus_tag="SCK06_000655"
     CDS             complement(17730..18578)
                     /locus_tag="SCK06_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1150682.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18602..19288)
                     /locus_tag="SCK06_000656"
     CDS             complement(18602..19288)
                     /locus_tag="SCK06_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150683.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19281..20315)
                     /locus_tag="SCK06_000657"
     CDS             complement(19281..20315)
                     /locus_tag="SCK06_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1150684.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20378..20634)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20710..21048)
                     /locus_tag="SCK06_000658"
     CDS             complement(20710..21048)
                     /locus_tag="SCK06_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG1150685.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21050..21406)
                     /locus_tag="SCK06_000659"
     CDS             complement(21050..21406)
                     /locus_tag="SCK06_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG1150686.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21412..22599)
                     /locus_tag="SCK06_000660"
     CDS             complement(21412..22599)
                     /locus_tag="SCK06_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1150687.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22790..23635)
                     /locus_tag="SCK06_000661"
     CDS             complement(22790..23635)
                     /locus_tag="SCK06_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1150688.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23869..24834
                     /locus_tag="SCK06_000662"
     CDS             23869..24834
                     /locus_tag="SCK06_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG1150689.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25211..26254
                     /locus_tag="SCK06_000663"
     CDS             25211..26254
                     /locus_tag="SCK06_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG1150690.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26316..26780)
                     /locus_tag="SCK06_000664"
     CDS             complement(26316..26780)
                     /locus_tag="SCK06_000664"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG1150691.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27026..27973
                     /locus_tag="SCK06_000665"
     CDS             27026..27973
                     /locus_tag="SCK06_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1150692.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28184..30256)
                     /locus_tag="SCK06_000666"
     CDS             complement(28184..30256)
                     /locus_tag="SCK06_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1150693.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30189..30566)
                     /locus_tag="SCK06_000667"
     CDS             complement(30189..30566)
                     /locus_tag="SCK06_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG1150694.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30695..31273)
                     /locus_tag="SCK06_000668"
     CDS             complement(30695..31273)
                     /locus_tag="SCK06_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1150695.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31405..32697
                     /locus_tag="SCK06_000669"
     CDS             31405..32697
                     /locus_tag="SCK06_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1150696.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32757..33875)
                     /locus_tag="SCK06_000670"
     CDS             complement(32757..33875)
                     /locus_tag="SCK06_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG1150697.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33889..35310)
                     /gene="arcD"
                     /locus_tag="SCK06_000671"
     CDS             complement(33889..35310)
                     /gene="arcD"
                     /locus_tag="SCK06_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG1150698.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35556..36677)
                     /gene="mnmA"
                     /locus_tag="SCK06_000672"
     CDS             complement(35556..36677)
                     /gene="mnmA"
                     /locus_tag="SCK06_000672"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG1150699.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37081..37680
                     /locus_tag="SCK06_000673"
     CDS             37081..37680
                     /locus_tag="SCK06_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG1150700.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37818..39104)
                     /gene="efmA"
                     /locus_tag="SCK06_000674"
     CDS             complement(37818..39104)
                     /gene="efmA"
                     /locus_tag="SCK06_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG1150701.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39375..39719)
                     /locus_tag="SCK06_000675"
     CDS             complement(39375..39719)
                     /locus_tag="SCK06_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG1150702.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39856..41001)
                     /locus_tag="SCK06_000676"
     CDS             complement(39856..41001)
                     /locus_tag="SCK06_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1150703.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11847 a         8652 c         7193 g        13473 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
       61 aaggagtcta acgttgtttg atataactta gactgatcgt taacttattt tttatatttc
      121 tacatagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
      181 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
      241 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
      301 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
      361 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
      421 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
      481 taatcagtta cctgtccgtt cttaaatacc acagaataat cttttttccc attttcaagt
      541 aaccgaagtg gaatgtcttt accaacttgc tctgtcactg cattactgat acctgttaga
      601 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
      661 gtgattcctt tatcttcatt tacaagcttc caagtaggtg gtatagctgt gtatgtttcg
      721 atgaatgaat caattctttc atctatcgtc ttcccagtat ctacttctgt ctcattactc
      781 ttagatgaat cgattctttc atccatcgtc tgctccgtaa ctgcttctgt ctcattattc
      841 tcaccggaaa ctctcttcac acccaaactt atatattgtc catctttagt gtgcatagtt
      901 aaaattccca cgcttttact aataaattca ctataaccag ttctttcaat agagacttca
      961 atgtatttca cactatcata ctcaaaagaa aaatagacat gtaaaagcat tttgttgttt
     1021 acatctacat aataatcctt tgactctact tgagaccttc ctatacggaa tacagcttta
     1081 ctgtctgata tctctatacc agcatcctta ttattaatct gccaatctcc aagtagctct
     1141 tctggcaagc tagtagtttc ttgtgaacac tttaccactt cactggtaga ggtcgaaaca
     1201 ttcacctccg cactgttaga agaattttcc aaggaatcag gagccttagc acaaccaaca
     1261 agtctttata tattgtgcag ccgatatttt cttcattcag tacacccttg aaatatgcag
     1321 tgttagataa agcagttcgt cctgatttgc tgaggcaccc atctcttctt gaacgtactc
     1381 cttgattttc aaggccgccc gatactcttt cggaaaacgc tgcttcactg aagccagcaa
     1441 aaaatcatcc ttctccggca actcttcctt cttaatcatt cgggcaataa aaaactgtaa
     1501 atgcgtcaca aatcttaagt aaagcatcga atctttatca accttttttt cgggaaacag
     1561 atatcctagc atagccaaga tatcctttag tgctcttacc gagagcatgg tttcttccat
     1621 atttgcttgt tcactttggg catttacaat gtgaaacgca atgtttccag cctcctcttc
     1681 tggcaactga acattcattt ttttgttgat caattggata gcatagcagc cgatctcaaa
     1741 ttcttttggg taatactttt gaatttcaat cagcaggcga ttttgaaaaa caatgccttt
     1801 ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag gtaaaaaata gacttttatt
     1861 cagcttaaag tcaaacttgg cgttggcata atcgataatt cgcttgacgc aaataattag
     1921 atcatccgga gcagcctcaa acagtttaat gtaatccttt tttagagcat cagaaagtga
     1981 atctaattta aatacttttt ggattttgtc agccggaacc aaatcgcctt tctttagttg
     2041 aaatccaatc cctttcccca tcaaaattac ttctttgcct tgcttatcta agcaaagaat
     2101 taaactgttg tttaacgttt taattactct cattagattc accgttctct ctaagctaag
     2161 ccatttcttg cgattacttt ttgataccaa aagaagcttt ctttttttat tcgttttaat
     2221 tctttaagat tctcatcggt tcggtcaaca tatataaaac cataacgctt attgaagcct
     2281 tgatgggtac taacaacatc aatcgctgac caaggtaaat aaccaaaaac atctacacca
     2341 tctgttaaag ctgcttgaat agccagaata tgtttggcta gataatcaat tcgatcctgg
     2401 tcattaattc gtctatcttc ctcaactatg tcgggtgccc catagccatt ttcagtaatt
     2461 aaaataggta aatcatagcg atcgtacaat ttcctcaatg ttaatcgtag tccagtaggg
     2521 tcaactaccc agccatatgg cgttttatct acataaggat tttcagctgc ccggtagaca
     2581 ccttcttctc ccaacatgat ctgttgatcc cccgctctag cggaaacatc tgatccgtca
     2641 tttttgctgg cagcgatggt ggcagttgaa taataattaa tcgcaataaa atcgggttta
     2701 ccggaggata aaattgcctg atcctctgcc tcaattttag gatataatcc acgatcattc
     2761 aaatagtttt caaacaaata attgtatttt ccccgaactg ctacatctaa gaaagaccag
     2821 cctctcaatg tttcccaatt atgggctgca attgcatctt ctggactgga ggtagcttga
     2881 tacatagccg ttaaattgat tgctggaccg atttttcctt taagattcaa ctgatgatac
     2941 aagtgaatta ctctagcttg agctaataaa acatggtgat taatttgaaa gagttctttt
     3001 tttgaaggca gctctccact tggtggcaaa ccaatcgctc caggatgaag gatcatcgtg
     3061 ttttgctcat taattgtcag ccaatagttc accttgtcac catactcagt aagcaatacc
     3121 tgtacatagt ccacaaaata atcgatagtt gctcgattta accaaccgcc ctgctcctct
     3181 agaaaataag gtaaatcgaa atggtacatg gtgacaatcg gttcgattcc ctgattcagg
     3241 cattcatcaa ttacttgatg ataaaaagaa attccctttt gattggtttt attattttca
     3301 atatccataa ttcgcgtcca agcgatggaa aagcgaaaag cttttaagcc taattcttta
     3361 aacagctgta tatcttcttt atagtgatgg taaaaatccg aggcaacttc aaaattagct
     3421 accccttctg tatgctgata cttgtcaata atagacggtt gtttcccatc ctcctgatag
     3481 gcaccctctg cctggtaggc actaatggag gcaccccata aaaacttctc tggaaatgct
     3541 gaaagctgtt tgtattgcac aaaaatcctc tccttaacta atttgcatca atggttcgtg
     3601 ttcctttaag cttccgcttg cttcactaat tcctgcgtaa ttttcactgt tggtcactac
     3661 cacaatcact gtatcatcgt agccagcgtt tctaatggca gcgaaatcgg cgttgattaa
     3721 taattgtcct gctgacacct gatctcctgc ttttacaaat acttcaaagg gttccccttt
     3781 taaattcacg gtgtcgatcc ccacatggat caataattca ataccttctt tagaacgtag
     3841 accaatcgca tgtttagatt ctaaaatagt aatgacttct ccctcaacag gtgaataaat
     3901 gctgttttct tctggatgaa tggctacgcc ctttcccaat agtcccttag aaaagacagc
     3961 atcgttcact tcagaaatag gacacacggt tccttttacc ggattttcaa caatgactgc
     4021 ttcggctatt gccgtttgct ctgattcatc agaagtatcg tcaaatccaa ttagataagc
     4081 aatgataaag gttgcaacaa aggcgattac acacgtaata attgcgtgaa caatattcat
     4141 tggattttct ccaataaagg ctggtaaggc tgctaagcca ggggaaacaa aggcatatct
     4201 gactaaaccg ctcaagccag catacaaacc agcaattgca ccaccaatca tggaagcaac
     4261 taatggtttt ttcagcttca aggttactcc atagagtgaa ggctcagtaa ttcccattaa
     4321 agcagtgatc ccagtggaca cagctagctg cttcaactct ttgtttttag ttttcgcggc
     4381 tacagcaaag gtagcacctg attgagcaat attagaagcc agcattcctg gcccattgat
     4441 catttcagaa cccgtactgc tcaattgcat agtagcgatt ggcgtcattg cccaagctgt
     4501 acccgtaacc accaaaaatg gctgcaaacc acccatcaaa aatggaatta accaagctgc
     4561 tttgccgtta atccattcag cccccattgc tataagatcg ttcagcaacg ttccaaacgg
     4621 tccaatgaca accaacgcta aaggccccat aattaaaaat accagcatcg gcttagtaaa
     4681 gaatttaata aacgaaggcg aaattcgatc cgcaaagggc tctacatagg acatgatcca
     4741 aacagtaaag ataatcggta aaactgatcc cgcataatct gcagctaata ccggaattcc
     4801 tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa atatttggat gaagcaaaat
     4861 gccagcaaca gtaatcgcca aaattgggtt gacatcaaac tttatggctg ccccataggc
     4921 taatagtact ggcataaaat aaaaggctgc atcagcaata gtattcaaaa tgatgtaagt
     4981 ttgactactg tctgatagta caccagtcaa agtcaagacg gccaaaatgg cttttaccat
     5041 cccggcacct gttatagctg gaatcatcgg tgtaaaggtg gtagaaatga cactaatcaa
     5101 tcttgaaata atactttctt gattgtctga agaggggccc tttttaattg aagttcccac
     5161 ttccttttga atttccttga aggctgtttg gacttcattg ccaatcacca cttggtattg
     5221 tccaccgcta tccaccacac taattacacc aagtgttttt tctaaggctt tcgtatcggc
     5281 tttcctttta tctttgagtt caaaacgcag tctggtggca caatgggtta agctgaccac
     5341 attttctttt cccccaacct gttgcaatac atttcttcct acttctctgt aatccattat
     5401 tctctctcct taaagcagta aaaaagacca agtcaaacca cgccccaatc aacattgaag
     5461 tgttagtttg tcttggtctt gcctgcataa tcagtaacat gccaataacg ttattttgct
     5521 gtaatccaat aatagcacat tatgatagcg caatcaacag ggaatttaat tttttatctt
     5581 ttcgtttgac cgtatagggt aatataacag ttagatcaca tcaactaggg tatatctgcg
     5641 tatacttact gctaaaataa gtatatctaa tattaactat accagtaaaa ccattcctac
     5701 ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa aatgcatata acggatcaca
     5761 caacttcaac aattctgctc ttgggaaaaa ttgatgaatt tggtgagtaa aggatggaat
     5821 aaacagcatt ccaacaatat aggcaacaat agtgatactt gtttttttgg taaaaatcag
     5881 taagcaatag ccaatttcaa tcattccgag atataaaggt atcgatagaa tgatttgggc
     5941 tagtattctt aaaaattaac tagcaaatgt ttaaactgat ttatttctaa tctacatgta
     6001 gtaagtattt caattccacc aattatcaat aaaaatacca aacatatcca taataatttt
     6061 ttctttttca tttcttttat attgttataa cagtgaataa cataaaaatt cccgacaatc
     6121 attgtaatat ttttttgtca cctgtattag tatttaattt ataggaaact ataaaaactc
     6181 cctagtgaat ttacaccttt gggagttcct gtttggacgg tatgcataat acatttaaat
     6241 caatttaaca ttaacacctc agcatatggt atctccacat atatacggtc tttctttgga
     6301 tagtactcaa tatttatgtt gatagcactt ccattgtcta atatagcttt agcccggtat
     6361 gtgttatctc cattttcatg agtaatcgtc acatcatcac atgatcgatt taagttgtac
     6421 tgttcttgta aaatctgtgt taccaatggt tttgatgact tctctaatcc agatgtacct
     6481 gtcatttttg aaaaaataat acctataata gccaagacaa caatacctat aatgacaaac
     6541 ttcttgttca tatttacttt attcaacttt ttatctccta tctccaaaat aatttgctct
     6601 tatccctctt atcagtacat tgacatccct aaaagtacac caaagattgc tccagctacc
     6661 cctgctatca tcaaaatcgt acccattttc tcatcttttt ctcgatataa gtagcccata
     6721 ataacagcaa cagcaccaaa tacaacagga ataaatgcta acgatactac catagaaatc
     6781 caaccaataa tcaaatacag cttccctgtc tcttgtccat gtacgatttg ttccttcatt
     6841 tcattaattg tctgctcagt acttttcttt actgtgtctg ttccggttgt ctcatctgtt
     6901 tcttgtagtg ctccacaaaa ctggcaatac ttactttcat ctgttatttc tttgccacat
     6961 tttctacaat acatgtcttc tcctcctaaa atcattcaat tgcttattag ttaactgttt
     7021 actttattaa atacatcaga aaacagctca ttcattggta cacttaatat ctcagaaagc
     7081 atcttcattt gatagggttt aggaatcgtt ctccctgttt gccacgaact tacacattgc
     7141 tgtgtaacat tcattttttt tgctaactcc gtttgagaaa gtcctttttc ttttctgtat
     7201 cggctgagat tacttacttt tttcatgatt ctcccctcct gttttattat atacaacaat
     7261 atgatgtatt tcaactcata aaatataaaa aacttcaaat accattacaa atacaacaac
     7321 ttgttgtact atactgagat gggggtgtat taatgaaaca gaaacaatat gcaaaggtca
     7381 ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga attagctgaa aaaatcttct
     7441 ctacacaaca ggcagtagca agatgggaaa attctgtaac agagcctaac ctcgatagct
     7501 taacagctct ttctagagcc ctaggaaccc ctgtaagcca ttttctagac aatgttgtcg
     7561 tagattatga ggaagagttt ttggctctct atcgttctct tagcactgaa gatgcagttc
     7621 gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa tgaacgcaat cagctattga
     7681 aagattgaaa aataaataac ccaccgaact ttaacggtgg gtgtattgag atgaaataag
     7741 attaagagct atggataacc tgtctagttt aatacaattg ggtcttttct cttccttact
     7801 ctccgtccat catctcttgt tacacttgta aattgataca gaggaactat cccagctcct
     7861 gataatatca attcttttat tgtttcatct atccctgccc ctttcgctaa atatattaaa
     7921 aaactactac ttgctttcac attgtatagt ttcttaatat ccttagaagc tcgttcaaga
     7981 gtgaatcctt gtttaacaaa gtagcctctg ttttttatta aagcattttt cacgctgtta
     8041 tccccaacat tacgtaggcg ttcacaaaca gtgttaataa gttctttctc tgtcattaga
     8101 tatgtccctt gccacgataa gccctgtaaa acttcatgtg cttcatctaa aatcaattgt
     8161 ttttccttgc tgagttctga tcgggaattt tgaggaaata ctcttttagc ctctttttcg
     8221 ttaaaacata tcttaaccca ttcataagaa aaatattgat atgtaaaatg aattttttcc
     8281 agaagtggaa ggatatcttc attcaatttt tgaatcacag aagttgcatt aaaaacgtct
     8341 aatcgataga cattgcttct cttatgatta aagtttccta acgctttctc ttcctttaac
     8401 ctatttaggt attcgagcat cttcaaagga acatcttcat catctaattt ttttatcaat
     8461 cctataaaag tcataagtga tagtaactta ttacatttat ctatgtcata agttttacca
     8521 tagtaagaac tcattttttt agctaattgt tcccccgaca aaatacaagt cacactttcc
     8581 ttactatcat caaaaaaatt aacatcagaa agcaagacat catagacacg tcgcatttcc
     8641 tctagataat tccttattgt cctgaaaagt ttgtctttca ttgattcaag agtgtcaaaa
     8701 agtacttttg attcctcttt caattttcga aaatgtgtag gatacaagtc taggttctct
     8761 aatagccaat taaatgtttc tacccgactg tctttcttta aaattagcat cacaactcca
     8821 ataaaatttt cactgatatc acaattactg gaatgacaat aaaaaatgga atgcccagtt
     8881 ttactggtaa aaatactagc agagggatta tgatcgttgt gaaacagaca tcttaagttt
     8941 ttacttgact taagtaattt attcatatca atggttaaca atctttcata aatctcatta
     9001 aaagacatgt cggaggaatc aaacaggcag tctttaaacc actgtttcat ttcttctttg
     9061 tcgtcatttt ttatcaactt aacaaaatcg gtttcattga ctagttcata ctctctccct
     9121 gaatcacttt gacaattttt agaacatgtc atggtaaaat caatcggaag ctcagtaata
     9181 tctaacatgt tgtcttcatt aattattatc actgcatctg acttatatcc tccaaaaaac
     9241 attctattcg aagcatttac attgtcatca cacccgggga tgttcttttt caagtattcg
     9301 taaacagcaa ccattttacc atagtcagta attgcttctt ttaatagaaa gacaatcttg
     9361 tatttttcta gattttcaga gtaccgaata cttttttgta tcgcaaacgc agaattatga
     9421 actaaattga tagataaaac cttttctacg cttaagtatc tactatcatc tttactcaaa
     9481 tctactacct tccccttctc atctttaaca gtgttatcga catccagaat aattactctg
     9541 gtttccacaa aactctcctt gtcattgcct ttattctttg tcttgcttgg aataaatgat
     9601 tgtgatgaat ttagtatctc tacaaactca tctcttgtaa cttctctata gttatctaaa
     9661 cacctatctc ttacatcagt tttttctttt tttgtaactg gcttctctaa taatttttct
     9721 tgatctacat aaattggaat attaatcatt gatgtttcca tttgtgtttc ctccttataa
     9781 aattctgtta aagactaaaa catacagata ataaaaacaa actctttatt actatatgtt
     9841 ttagtcttat ttgtttactc aacctttata ccgttccatg aaactatcaa tatcagattt
     9901 tttaaatcta atgaccgtat caatttttat aacaggtaac ccgtattgag aaacatattt
     9961 tctaatcgtt cctactgata cacctagata ttcacttgcc tgctgatagt ccatatactc
    10021 tagagtgact ccagaaataa tgtcttctag cataggcttt agcactgtaa ttatctcttt
    10081 cacttgttgt tcaacttgta tagaaacaat gctatcaatg atttgtagag acatatttcc
    10141 ccctcctttc gtgattgctt gaacaaattt tctaagaatt atcttcgtat tttttgctca
    10201 aaataaaagt attttgttat taaccgatta atcaaacaaa aaatgattac cagttcatcg
    10261 acatacacat aatacctcga taaaaaaggg gtgtcaacag ctttgtaaaa tttatttgca
    10321 acaccccttt tttgaggtat actaatttta ggaggtgtgg tcaatatgat aaaacgtcca
    10381 ccaataaatt atttagaacg aaaaaagata ctcggaacaa aaataaaagc tataagaaag
    10441 agtaaaaaat taacacaacc agcatttggt cttatgatta acaacggtca attaattgac
    10501 aaaaaaacta tctacgaatg ggagaaggga acctatttac caataccaga gcgattatcc
    10561 cgaattgctg atttgggaaa tatgagtata gaggagttag tttgtggcaa tgtagaagaa
    10621 tatattttgg gaattattct ttatagagat agcattgtac tagatggtat aacatttcca
    10681 gataaaaacc tgttccagca tcttcgtcaa cagttcccac cagtacactc taatttagat
    10741 acatggctgg atagatattc caaattagaa ccagagatgc aagaatttat tgctaacaag
    10801 acttgtaata aagttaaaaa tgaaaaaatt agtcttttca atatcttaaa aatagaagaa
    10861 ttattcatca atgctattgt agaagaattt gacaataaca ttcttttcct cactagcagt
    10921 atagaagaac tactagaaag aatggtagat gaatggctac ctattcaatt gaaagacatg
    10981 tcttatcccg aagaagctgt gagagaaatt actgataata taaacaagct agaacaaacg
    11041 atttcatcaa ttgggaaaaa atacactaag aaaaaaatga agggaggtga taccatctag
    11101 tggtaagtac tccttgtaaa ctaagaggag atgatttaat ggcaaaaata tcaaagtaca
    11161 aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca cattgccacc gatccacgaa
    11221 ctggtgaaaa aataaatact acgagaagag gttttagtag caaacaagaa gctcaaaaag
    11281 attatgacga gtacagacat caaatattat acggtgttaa aaagaaagca cctgatatga
    11341 catttgaaga cctgtataat gaatggataa ctcatcagcg taccagtgtt aaagcttcaa
    11401 caatcgcaat ctcagtaaga tacgcaaata atcaaatact tccagcattt ggtaagctta
    11461 aactatcgaa tatctctgtc ccatattgtc aaaaagtggt agatgaatgg cacagtaaat
    11521 atgaaagtta cgattatatg agaaaacaga ctgcacaaat tttacgctac ggggttgcaa
    11581 tgcaatatat tgacaataat cctatggaaa aaacacttct tccacgaaaa aaagagtatg
    11641 agaaaaatcg taaattttac tcaaaagagg agcttaataa tttactggat gcttttaaag
    11701 attttggtaa catgaaacaa tacgccttct tcagattatt ggcatacaca ggtatgagaa
    11761 agagtgaagt actcgcttta cagtggaaag acatagacac gtttaataaa gaactacatg
    11821 tcaataaaac tcttgctgta gatgaatttg gtaaagttat aattcaatct ccgaaaacaa
    11881 gagcttctag acgagttatc tctttagata ctgagactct ttctatcctc aacaactgga
    11941 aattacagca aaaagaagag tatttaaaac ttggatacaa tactagctct aaagagcaac
    12001 atgtttttac aacagttaaa aacactctat acattccaaa cactgttaat gattggttaa
    12061 gatatatttt gaaaaaatat aacttgcctc gtattacacc tcatgggttt agacatacac
    12121 atgctagttt attattagaa gcaggagaat ctgtcaaggt agttcaacaa cgattagggc
    12181 atgaaaatag taaagtgaca ttagacatct atgcacatat tacaaataat gcacctaaga
    12241 aaacaggtca agactttgct gatatgatgg ctcatcagta aaagagctgg tcaaaatctg
    12301 gtcatttcta gtatacaggc aaaacaaaaa agctagaacc cttatatatc aagggttcta
    12361 gctcctataa caggtttacc caacactgcc ttccatctca taactgatca atcgattgag
    12421 ttcgactgca tattccattg gaagttcttt ggtgaatggt tcaacaaagc ccatgacgat
    12481 catttcagtt gcttcagatt cggtcaggcc tcggctcatc agatagtaga gttgttcttc
    12541 tgagattttt gagactttgg cttcgtgttc taaagagact tggctgttgt ggatctcatt
    12601 gaatgggatc gtatcggatt tcgaccattc gtccatgatg atcgtgtcac attcgatatg
    12661 agagatcgaa cccgcactgt ttttggcaaa ggttacttgt cctcgatagt ttacttctcc
    12721 gccgtctttt gaaattgatt tagagacgat agagctagat gtatttggtg cattgtggat
    12781 catttttgca cccgtatctt ggatttggtt tgctcctgca aaggcgatgg agagcatggt
    12841 gcctcgtgca ccttttccat ctaagtaaac acttgggtat ttcatcgttg tttttgcacc
    12901 taggtttccg tcgatccatt caacggttgc gccttcgtat gcttttgctc gtttggttac
    12961 taagttgtac acattgtctg accagttttg gatcgttgtg taacggcaat acgcatcttt
    13021 tcttgtaaag atttctacga tggcggcatg caggctgtta cttgaataag taggtgccgt
    13081 acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca atgatcagcg tacgttcgaa
    13141 ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt aatggaacat cgactttgac
    13201 gccttttggc acgtaaataa aggtcccacc tgaccatacg gctgaattca gcgctgctaa
    13261 tttgttgtcg gttggcggta caagttttga aaagtactct ttgaataatt cagggtattc
    13321 tttcaaagca gagtcagtgt cagtaaagac gattcctaac ttttcgaatt catctttcat
    13381 attatggtac acgacttctg attcatattg tgcggaagca ccggctagat aagctcgttc
    13441 agcttctggt ataccaatct tttcaaatgt atctttgatt ttttctggta cgtcttccca
    13501 gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg atggcatcga aatcgatgtc
    13561 tgaaaggtcg gggccccatg tttgcattgg cattttgtta aatgtttcta gagatttcag
    13621 acggaattca agcatccatt ctggttcgcc tttgattctt gacatctcac ggatcacttc
    13681 ttctgtcaag ccttctcctg tactgaaaac cggttcgaca tcatcgtgaa acccgaattt
    13741 atattcttct aattctggta cgcccatgtt ctcactcctc ttccttgtgg taatgatgga
    13801 aagctgcggt accatcgttt tccattgctt ttcctagtgc tttccaggca agtgtcgcac
    13861 atttgatccg agcgggaaat ttagctacac caccaagcat agcagcatcg cctaattggt
    13921 cgggatcttt gacttctttt ccttggacaa gctgtagaaa gtctacagtt aaatgttctg
    13981 cttcttctat tgattttccg atgacggcgt ctgtcatcat cgaggcactt gccgtactga
    14041 tggagcagcc actgccgtca aaggcgatat ttttgatgat tccgtctttt acatcgactt
    14101 gcaattcgat cacatctcca catgtggggt tgttcaattc gatcttgctt gtcgagtcag
    14161 tcaagcttcc acgatgatgc ggatgggaag aatgatccaa gatcacttga cggtataaat
    14221 tgtctaattt agatagtccc atgttggaaa aactcctttg ttgccatgat tgcttcaact
    14281 aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc tcgcgcgagc agtcgctgat
    14341 acttcaaggt acttcaatag cggttgcgca caatgatgac ctgctctgac ggctacccct
    14401 tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc catccaaatt gaatgcaacg
    14461 actcctgtcc gaaaattcgg gtcttgtgga ccataaacag tcaatccatc aattgcttgt
    14521 aattttggca acacataagc ggctagttct tcttcatatc gatggatatt gtccataccg
    14581 agtttttgca aataatcgat tgcatggcct aatgcgatag cgccggcgat atttggtgtc
    14641 cctgcttcga atttccaagg tagttctttc catgtactgt cgtataattc gacaaaatcg
    14701 atcatttcgc caccaaattc aacaggttcc atttcttcta accattttcg tttaccatat
    14761 aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc tgaaagcaaa gaaatcacag
    14821 tctagtgctt ggacatcaat ggccatgtgc ggggcagatt gagcaccatc tactacgatg
    14881 accccgccgt tttcatggac aagttcagct aattcttgaa caggattgaa gacacctaag
    14941 acattggata catgagcaat ggagacgatt ttggttttgt ccgtaatctt tttcgctgca
    15001 tcctccatat ccaggaagcc ttcttctgtt agttcgatat atttcaagac agcacctgtt
    15061 cgttttgcca actgctgcca cgggataaca ttagaatgat gttccatgta agagagcact
    15121 atctcgtctc ctgctttgat atagcggtcg ccgaaggttc tggctaccca attcaagctt
    15181 gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt ttgcatggat gaacgcccgt
    15241 actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt ctgctaacgt atggacaccg
    15301 cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta atgcctggag tacttgtttt
    15361 ggcttttggg tcgtcgctgc attatccaga taaactaaag gttcatcatt gacgtcttga
    15421 aataagatag gaaaatcatt gcgtagcttt ttagcatcga tcatccattc aacttccctt
    15481 cgattacttc gactaattct ttccgaactt gttctacagg gattgctgtt aagacagaac
    15541 cgaggaatcc tcgaatcact aagcgctctg cttcttcttt atgcaatcct ctactcatca
    15601 gatagtacat ttcttctgga tcaactcgtc ctacactggc agcatgtcca gcagttactt
    15661 cgttttcatc gatcaaaaga ataggattcg catcaccacg tgctttatca gaaagcatca
    15721 agacacgact ttcttgttgg gcatctgcac cttttgcccc tttcaagatg tggccgatac
    15781 cattaaaggt caatgttcct ttttctcgga tcactccgtg ttgcaaaata tgcccgatcg
    15841 tatgaggggc tttattggtt actcgtgtat cgattccttg gatttgtctg cccgagctga
    15901 ttgccacgat tttgacttct gcgtgtgcac cttctcctgc taaatctgag tcaaagtcag
    15961 cgatcacatg accgtcattc atgacaccaa ttgcccagtc gacagaagca tctcgtaaaa
    16021 tgtgcccacg gcgattcata taagaagtaa tattttctcc taattgatct acggctgaat
    16081 atttgatttt tgaaccagct ttagcgatga cttccacgat gatattacct gatgattttg
    16141 ctacttgttc tttggttgtc tggaaacgtt ccagataaga aaattcacta tgttcatctg
    16201 ccacgatcaa cacatgcttg aagaagtgct catcggatgc gccatcttga atgaataagg
    16261 actcgagtgg ttcatcgatc acgacatttt taggcacata caggaaaata ccgctgttca
    16321 taaaagctac atggagtgca gtcaattggt cttcgttcat gtttactgct tttttcatat
    16381 aatattcttg gactagttct ggatattcga tcattgcagt gaacaagtcg gtaaaaatga
    16441 ctcctttttc tgctaattcc acaggcagtt gttcaaaaat cgtccatgaa ccttgttgga
    16501 caagcacagg attgtctttc attgcatcaa atgccggaat cgtgcctgtt tcactgatgg
    16561 tttgtgtttc tttcacatca aataacgacc agcgatggaa tttcacccga tcgatatgcg
    16621 gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg gaaggttctc atccaatcag
    16681 gttcgccttt acttaaagaa aaatcattta ccgcgtcaag ataatcgagc cagttttttt
    16741 ctttcattct tgctccccct tacgcttctt cttctttata gtcgattcct aattctttac
    16801 tgataccagc gtagccttct gcttccagac gtttagctag gtcagcattc cctgtcatga
    16861 ctactcggcc ttccatcatg atgtgaacga catcaggtgt gatatagttc aataatcgtt
    16921 gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg catttcattt actcctttag
    16981 caacgacttt caaagcatcg atatccagac ctgaatcgat ttcgtctaaa attgcaaatg
    17041 taggttctaa catcaacaat tgcaggatct cattccgttt cttttctccg ccagaaaaac
    17101 cttcgttcag ataacgttca gccatttctt ctggcatatt tagtaaagcc attttttcat
    17161 ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt ttcatcacgt ttcgcattga
    17221 tcgctgcacg catgaattcc gcattcgtga tacctggtat ctcacttgga tattgcatag
    17281 ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa gtctaaaaca tttttcccgt
    17341 caaacaaaat ttcgccttct gttacttcat agtttggatt gcccatgata gcggcagata
    17401 aggtagattt tcctgtgccg ttaggtccca tgatggcatg gatttctccg gttttcattg
    17461 ttaggtttac gcctttaagg atttcttttt cctcgatcga cacatgtaga ttttttattt
    17521 ctaagacaga catgcattat acccccaaat tctctttcgt tctcactcat tttagactaa
    17581 ttgagaatga agcgcaatga ccttccttac taaaatcaaa tatcttttat ttattattaa
    17641 acaattttga tttctaaatt agaataggta taaaaaagat gagaaataag ccattacaga
    17701 cttatttctc atcttttttg ttccatacct tattttgcat cagcctttac tggtttaaca
    17761 gagccgcccc atttgtctgt gatccattct tggacttctt tgcggtgaag cacttcaatc
    17821 agttttttga ttttgtcact gtcttcatca cctttacgag tcgcaataat attcacgtaa
    17881 ggagagttgt ccttttcaag caatacggca tcttctaatg gattcaaccc tgcgccgtaa
    17941 gcgaagtttg cgttgattgc aactaggtca ccttcatcat tttggtacgt agaagctaaa
    18001 agtgatgggt cgatcgtatg gttgaacttc aagttcttag ggttttcttc aatatcatcg
    18061 aatgttgctg tttccagatc tactccatct ttgactttga ctaagccagc atcttgtaga
    18121 attgtgatga tgcgtcccca gtctgattca gaatttgaag taatgattgt tgcaccatct
    18181 tttaattcgg aaacatcttt gactttttta gagtacaagc ccattggttc gatgtggaca
    18241 gcaccggcat tgacaaagtc atatcctttt tctttgattt ctttgttcaa ataaggaata
    18301 tgctggaagt aattggcatc aatgtcgcct tcttccaaag attggttcgg taatacatag
    18361 tcatcgaatt tgacgatttc aagatcgatt ccttcatctt tcaataatgg tttcacatgt
    18421 tccaaaatct ctgcgtgcgg tgtcggtgaa gcgccgactt tcaatgtcgt gtcgtctgct
    18481 ttatttgctg aatctttgct gtcgctgttg ttaccgcatg ctgctaatcc tacagtcagc
    18541 aaaagcgctg ctgcaaatcc aaaaattttc tttttcatat cctacatccc cctatatcct
    18601 attatctttt gtctgttttc ttagttaaga aatcaccgat tccttgaacg ataaatacga
    18661 ttagtaaaat aattaaagtc gcaacgaatg tcactgttag attaccacgc tggtagcctt
    18721 cctgccaagc aagacctcca agtccacctg cgccgatcgc gccggccatc gcagtaaatc
    18781 cgatcatcga gatcgttgta actgttaatc cagaaatcaa tgcgggaata ctttcaggta
    18841 tcaagacctt ccagatgatt tcccagtaac ttgctcccat tgcatctgct gcttccagta
    18901 caccagggct gacttcacga aaagcaatct ctactagacg ggcataaaat ggtgctgcgg
    18961 ataaaacaag agctggaatg gctgcttgcg cacctagcat agtgcctacg attgctttag
    19021 taaatggaat gatcaaaacc atcaggatca taaatggcgt tgaccggaaa atattactga
    19081 tgatcgatac gattccataa atgattcgag catacggttt gtttttgcgt ccaatagagt
    19141 aaaggatcaa tcccaaaact aatccgatga tcgtcactaa gaccattgag attaatgtca
    19201 tatatagtgt atcaaggata gcctgattca tcgtttccgg gttgatttgt ttaaaatcta
    19261 gatatgtttc tgaaaagctt ttatccacga tggatcacct caattcctac tccttgttct
    19321 ttgatgaaca cttctgcttg atctaattct ttggtttcac cagtcatcaa gacagtcaat
    19381 gaaccaaagg aaccttcttt cacttgtttg atctttccat acacaacgtt gacatcgatg
    19441 ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg catttctttc gctaaaggtc
    19501 aaagttgcca aacggccgct tggattttct ttgatgagtt cagctaatag ttctgtcgaa
    19561 tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg ttacttcttt tttcggttgt
    19621 ctgaagacag tcaatacgtc tccttcttct acgactttcc ccaactccat cacagcgact
    19681 ttgttgcaga ttttgcggat cacattcatt tcatgtgtga tcaagacgat ggtcaaattc
    19741 aatttcttat tgatcgctaa caataagtct aatacttctt ctgttgtttg cgggtccaat
    19801 gcgctagtag cctcatcaca aagcaggatc tcaggatcat ttgccaatgc tcgtgcgata
    19861 ccgacgcgtt gtttctgtcc gccggaaagt tgtgaaggat atgcttgtcc gcgtccttct
    19921 aacccgacta aacgcaacag ttcttctgcc cgttctttac gtttgctctt tggtacgcca
    19981 gctaattcca gcggcagctg gatattttca agtacagttc ttgaccataa caggttgaaa
    20041 tgctggaaga tcataccgat tttctttcgg aatgtacgta attctttgtt ttttaatttt
    20101 gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg gaagctcaag tccattcagg
    20161 agacggacta atgtactttt tcctgcgcca gaatagccga cgattccata aatatcccct
    20221 ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt ttccgttttt cccattgaat
    20281 gtctttttga cattcgttag ttggattaat gacatgccat tcacatcttt ctcttatgat
    20341 ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa aaggacgaaa gcttgaactt
    20401 ccgtgttacc accttgattt accgttactt cgcaataaca gcctcttcca gtacttggac
    20461 ttagcttctt tccgtatact gtcgcgctgt aacgggcact cccgtagtaa gtttgcttac
    20521 gctcgcttac tctgctctaa gaccatcttc catctcattt tcgttaccca ttttcagcaa
    20581 acagggctct cttcaaacgt ccgtgacatg tactcttctt ttcaaagctt tctcgtttca
    20641 tataggattg cttatgatta tagcaactgg aagaatcagt gtcaactttt ttcaagtgat
    20701 tttctgattt tataattcgt caaattcttt tggatcaaca tctttgaaac ttaagatcca
    20761 cgcattcatt tcatctgaat cattcaagat attgatatct tcaccgattt tatcattaac
    20821 agaagaaact gtacccgtta gcgggctggc aaattcattg accgcttttt ccgcttcaac
    20881 ttcgatcaga ggttcatctt tttttagcga ttgtcctgct tttggcagat tggcaaacgt
    20941 gatgtctcca agttcttctt gcgcttcatt tgtcaaacca acacagtatt cttttccatt
    21001 aaataagatc caaaggttgt cctttttctt caaatgcttg cctgccattt tatatcagtt
    21061 ctccttcata aattttttca ttgaatccaa ttgcaacaag ctggtcctct cgtaagatca
    21121 gtggtcgctt gatcagcata ccatctgtcg ataagacttg gctcgctttt gaagccgtaa
    21181 actcgcctac ttgatctttc agtccaagtt cccgatattt catcccgctt gtgttgaaca
    21241 ggcgacggat tggtacttga ctcgtttcta gccaagtctc aatcgttgag gccttcggta
    21301 cttctttgat catatcaacg gtttgataac taacgccatg ctggtctaac cacgctttgg
    21361 cttttttgca tgttgaacat ttcgggtacc agtaaaaggt gtacaatttt cttacctcct
    21421 tgaagatctt gcaggtgctt cattttggta tctcatcgat aatatcaacc cgataccgat
    21481 catgttccct aaaatcgagg atcctccctg actaatgaaa ggcaatggga tccctgttaa
    21541 aggaagtaat ccgatatttg ctccaatatt ctcaaagaca tggaacagca gcatcatgat
    21601 caaaccgctt gcgatatagg cgtaaaattc attattcgta tcgaaacaca cccgaatcat
    21661 acgatagatt aaaataaaat acaagaaaat gacgaatgca ccaccgataa agccgaagtt
    21721 ttcaccgata acggtaaaaa tcatatcgga ctcacgaaca ggcacataga tattgcttac
    21781 gttgaatcct ttaccaaaca agccaccggt accaattgcc aaaagtcctt gggccggctg
    21841 ataactcatt cctgacgtat catggaaagg gtccagccat gaatcaatac gtgcaaactg
    21901 ataagatttg aatccaactt tataaagcaa gtctcttccg ccttcagtcg taaccaagaa
    21961 gatcgttcct gctccaacga ctactgccaa gattactaca ggtgcgatga tgcgccaaga
    22021 gattccagac atcaggaaaa tcccgccaaa gatcgcaaga aagacaagca tcgttccaaa
    22081 gtcatcttgg gccatgatca atgctaaaac cggtattgtc acgatcaaga gcttcccaat
    22141 cagccaaaag tccgatttca tcgtccgttc ttttacttgc gtattatgtt tagttacgat
    22201 cagtgccatc atcaagatgt acgcaatctt catcaactct gctggctgga aagtaaacgc
    22261 accgaatcga aaccagtttt tagaccctgt tgaagcagct aaattctgat catagaattt
    22321 gagcaagagt cccattacca ctaaccccaa accataaagg taaggagtca gtttccagat
    22381 ccatttggaa ttcaaatgca tgatgacaac gattgctgcc gctccaacta aataccagac
    22441 aacttgcatc cctacgcctt tcaagactgc tggattgttc ggatcatggc taagtgctac
    22501 atataatgag agcagaccaa tcaaacataa cagaaagact ggtaaaatca cgccataatc
    22561 aatacgatta tcgttcgaag cttttaattt tttttccatg tttcatccct tttcttaaaa
    22621 accatactgc tgattattat agcggtccag ttagataatg gaaagtcttt ccatagacat
    22681 ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc aaatgcagta caaaagccta
    22741 ctgcttatct ttagtgtatc atagaacttt ttttgataca tgcaattatc taccagctga
    22801 gattctcctc gctgtgagaa gtttctgtgc tttcattgta tcgaaggatc tcttcggcaa
    22861 tctctagcag ctgatcagaa accagattgg ttcgttggat gaaataaaaa tatcgatgga
    22921 aattcaacgg agtcattggc acattttctg gaacagagcg cttagacaaa atcgagcgac
    22981 cgattccttc cttcagcaac gcaacaatta catcgttact tttcacgatc atcttcttat
    23041 cttggatatt catctcttca aaataacgct gcgtgtaatg aaaaacacca gagtttgctt
    23101 ctcttacaag ccacaatttg ctttttgggt ccccagcgat gactagctgg tcttccatca
    23161 aagagtatct ttgtacgccg gaagtctcta atggcttttc gataaaacca aaatcggatt
    23221 catgtttcgc tacttgttct aatgattcat gagaattcgt gatttcgatc tcaaaatgca
    23281 ctttaggaaa ccttttgatc agatgtttca tcaactgagg caaaagatag acagcaaagg
    23341 tgtgggaagc aacgatccga catacttctt cttcattttg ttcattgagc aatttttgct
    23401 gagtttcttc ccattcttct aacaaaacca gtgtccgttc atataataag tcggcttgct
    23461 tagttggaag catctctttt cttccgttac gttgaaaaag atcgatattc aattcccgct
    23521 ccaactgttt gatttgattc gagactgctg gttgagataa aaacagtact tctgctgctc
    23581 tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt taataactta aacatggcac
    23641 cacttccata actttttatg ataattacta taaaaactat ttgatttaaa tatacaaaaa
    23701 accatctata atagaaatgt acaacaaaac agttgctgat ccaactagta acttagatga
    23761 agacaatcag ctgtctgctt gctcgtaatc atacattcct attcatttta caatgtattc
    23821 gttcatcttg gtactaaaat ctattaaata aaaaggagaa tattatatat gaagaacttt
    23881 ttgaaaatta ttccgatccc gatctgcggg ttgatccttg ggttagcttc ccttggaaat
    23941 ctgtttaaag actatcacca tgttgcttta ggcaatctaa ctggcggtat ctctatgatt
    24001 cttatgatat tcatttttgt taaattgttt atcttattcg aacacacgaa acaaacgttg
    24061 catgatccaa tcatcgcctc cgtttcacca acttttacga tgtcactcat ggttatttgt
    24121 acttattttg tttcatatga aagtattgca ccaattgtca aattcatttg gctgtgcgct
    24181 gttatcttcc aagtgatcct tgtcttttat ttcaattatc accatgttat caaagctgat
    24241 ctttcaattg aagcaattta tccaagctgg tttatcattt acgttggatt tggggtcatc
    24301 actgtaactg ccgggaactt cttcccactt attggaaaaa ttttcttttg gatttcattg
    24361 atctgctatg cgttcttatt gccgatcatc atcaaacgtg tctttttcgt aagaaatatg
    24421 gctcatccga cacgtccgct gattacgatc gttgcagcac ctggttctct ttgcctaaca
    24481 ggttatctta aaaactttgc tgaacctaat ttttatcttg tcgtagcatt gttcgcttta
    24541 tctcaagtat tgtatttcgt tgtgctgtct atgcttccac gtttgttcaa gcttcgtttt
    24601 tatccaagct atgcagcctt tacattcccg cttgttatct ctgcaacagc tatatttact
    24661 actgttcatt acttcaacag tttaggtatc tatagtagcg tattggattt cttaaaagtt
    24721 gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc ttgctcatta cgtacgtttc
    24781 ttaattaaag aacatagcaa acatggaaaa tttgtttcta aaaaaatcgc ttaataatcg
    24841 attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacggcc aaacaaaaac
    24901 agctgttcat gtttttgtac tggttgggct tattaccgca gatgcaagct tttgaacacc
    24961 gtttaccaag aggtcgtaaa agaagtggtc agcttcaaga aataagaagg aatttacgaa
    25021 aattgcttct caaatttttg tgaaatttcg gcttatttcc gtagaagcta cttctggaac
    25081 accgtttatt cgaaacaaga ggtcgtacaa gacttttgtc acgacctctt gttcttttgt
    25141 caaaatactg gtttagtgct actatgttcg agtatgcttt tagtatttat tgacgaaagg
    25201 aaccgatcaa atgaaatcat tgtcacatcc aataaaatct gtaaaggatt cagcggtccg
    25261 cgaaatctta cccggtttac tgctttcttt tgccatcgct gccttcagca ggcttcttgc
    25321 tatttatctg ccacagttag gtggagcgac tattgctatt ttattgggta ttttattagg
    25381 aaatactttt tttaaacagc ctgttctagc aatcggcaca aaatttgctg aaagcaaact
    25441 attagaattc tctgtttttt tactaggtgc aactgtcaca gctcaaacga ttgctcaaat
    25501 tggtgtaaaa ggaattgttt ttgtgttact gcaaatgtca gtcactatca tcggtgctta
    25561 cttgattggt aaaaaactgc aattcagcga tacttcttct ttgatgatgg ctggaggaaa
    25621 cgctgtttgt ggttcttctg ctattgcttc aatcgcccct tccatccatg cgaaagagga
    25681 agaaaaagga cagatcatca cactagtcaa tctactgggt actgtgatga tgttgactct
    25741 cccttttctt ggattacagt tgtttggtgg aaatctcatt gaaaaaagtg cacttttggg
    25801 tggtacactc caatctgttg gtcaagtagt cgctggggca agcttacttg acaattctgt
    25861 tgtcgaattc gccatgctat ttaagattct acgtattatg atgctcgttg ttgttgtcta
    25921 tctgtttgaa aaattcatgc acaagaaaca accagctctg tttgaaacgc caactgatac
    25981 gcagaaaaaa cgaaaattac cgcttccttg gtatgtatta gggtttgtga tcttttgttt
    26041 ggtcaacagc ttgttttctt tacctgtagt tattgaccaa tcggcacatt ttatcagcag
    26101 ctggtttgag atcactgctt tagcggcaat tggtctgcgt ttagactttg caaaattcat
    26161 gaaagaaggt acacgcttct tatcttatgg attagctgtc gggcttctcc aaacagtgat
    26221 ggctttacta tttatttggc tgatcaaagt ttagaatgaa catgaaaaaa cagttgtgat
    26281 tctcgcatgg aacaatgtcc gaatcacaac tgtttttaat tttgcagata tccttctaac
    26341 aacaacaatt ggcgtttgat ttcaacccca tctattgcgt ttcccatata tcctgtcagt
    26401 tttccatttt gcccgatcac tcgatgacaa ggaataacga ttggcaatgg attggttcga
    26461 tttgcctgcc caatcgctcg cacagctttc ggactatcaa cagcaacggc tacttcttta
    26521 tacgttcgga tttctccata aggaatggtc gacaaagctt tccagacatt ttgctgaaaa
    26581 ggcgtacctc tctttataga taaaggaaca tcaaattctt ttctttttcc cgcaaaatat
    26641 tcttcaaact gttcttgtgt gcgcgttaaa attcgctgat tcgctgattc gctttctgaa
    26701 attggctgaa aagatacagc agtcaatccg gattcatctg catctaacca taaaaagccg
    26761 ataggtgtgt gtattttcat tttgtttgct cctagtcaat agactggaac ttcctccttt
    26821 ttctctatcc aaaactactt gccaactaat aaaaaatgtt cttaaaagtt tgataaaccg
    26881 aggaaacatg gtaaactaaa tacgttagtt tacaaaaaga atacctgttc ttttacgtaa
    26941 aatcaaattt taaaggtgtt atttaaggtt tattaaattt tgaatgtaaa ccatatataa
    27001 acatacgatt gaaaggatag aaacgatgaa agtatcaatt gttgtcccat gttttaatga
    27061 agaaaaaact gttcctcttt tttttgatga ggttgaaaaa ttcaaaggtg cccatgagtt
    27121 cgaatatttg tttattgacg acggatcaaa agacggtacg ttagcagcaa tcaagaagct
    27181 agcctcagct tatccttatg tccgctttgt ctcattttcc agaaattttg ggaaagaagc
    27241 ggctctgtac gctggattgc aagcttctac aggagattta gtgaccgtga tggatgttga
    27301 tctgcaagat ccgcccgaac tgcttcctga gatgatcaac cggatcacta cttctgatat
    27361 cgattgtgtc ggcacccggc gttcgactcg tgaaggcgag cctgcgatac gcagtttctt
    27421 tgcgaaaaaa ttctaccagt tgatcaataa aatcagcgat acagaaatgg tcgatggcgc
    27481 tcgtgatttc cgcttgatga ctcgtcaaat ggtggatgcc gtcctagaat taacagaata
    27541 caaccgattt tcaaaaggat tatttagctg ggtcggattt aaaacagaat atatttctta
    27601 tgaaaaccgc gaacgtgtgg ctggcgaaac atcttggtcc ttctggaaat tattcaacta
    27661 ctcgattgat ggtatcgtga acttctcgga tgcaccgtta acgattgctt catttatcgg
    27721 cgctttgtct tgtgtcggtt ctggtattgc aattttgttc attattttgc gggcattgtt
    27781 gtttggtgat cctacatcag gatggccttc catggtctcg atcttcctgt tcattggtgg
    27841 aatccaattg ttatgtcttg gaatcatcgg gaaatatatt gggaaaatct tcttagaaac
    27901 gaaacagcgt cctgtttata tcataagaga aacagaaaaa gtgaagccaa atgaattgaa
    27961 gcctcaccaa taatatacgt caaatgaagt aaacattttt ttaaatgaaa aaggactgtg
    28021 acaaaagtct tgtcacagtc cttttttccg aataaatggt gttccagaag tagcttcttc
    28081 ggaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    28141 tcttgaagct gaccacttct atcacaacct ctttcatatc ttattacttc ttcattgctg
    28201 cttttagtgc tgctgctaat gggctttctt ctggttcttc tgcttgtgca tattttttca
    28261 gcaagcgtcg ttcttcatgt ttggtcattt tcttcttctg ctctttttta tcaagcaatt
    28321 tttctgtcgt tccatcgtat ttacaacgga aataagcacc gtttttacct tcgatgattt
    28381 ccattttccg gtgacattgc gggcatcgat gattagagac ttttggatct tttcttctgc
    28441 ggtagttgca atctgaattg gtgcagacat agattttgcc gtcacgtgtg tttttctcgc
    28501 gcagattggc cccacattct gggcatttct tttgggtaat cgagaaatct tgatattctt
    28561 gtttgcttga tttgatctca ttcaccaagc gttttgtttc tttttcgatt ccattcaaga
    28621 aggttcctgc ttgtttcttt cctgcagcaa tagcttcaag gtctttttcc cagctttctg
    28681 tcaaatctgg tgttactaaa gagggattta ccaatactaa taattgctta cctttaggag
    28741 atacttgcag tccttgaggg gtacgttcca tcagttcaga tttgattagt ttctcgataa
    28801 tctctgcacg cgtagctggg gttcccagac tgtatttttc catttgacca agcaagcttg
    28861 cttctgacaa tggtttaggc ggtgcagata gttctttttt gatggcgaaa tcgggattga
    28921 ttctcattcc tttttcccat ttgactgttt ccgtctcagt ttcagtttcg cctttccatc
    28981 ctgcttggac gacacgattt tgccgaaaaa tgaatcgttc tttaccaaat tcaaccgtaa
    29041 cttttgtttg gctgacttta tggggttgag caaacagtcc taaaaaacga gagacaatca
    29101 tttgatagat ttttcgctct tcattactta atttttcata acgtgctctg ttttcagttg
    29161 gcaaaagtgc atggtgatcg gtcactttac tgtcttggaa tacttttgtc tgttggacga
    29221 tggctccgtt tttcaaatac tgcttgacct caggagcaaa gtcgctgact gcttgcaatc
    29281 gttctttcat cgtacttttc atgtcatttg tcagatattt gctgtctgta cgcggataag
    29341 taacgatctt atgggtctca tacaaacttt gtacaagact taatgttttc tttgcagaat
    29401 agccatagcg ttgattggct tctctttgga tctctgttaa atcataaggc aatggcgcag
    29461 attccgtttt taatttttct tggacttctg tgacagttcc tttgcttgta ctgatcaatt
    29521 gaacaagttt ttgtgcttct tcttgtgttt ttaaagcaaa ttgatttttt tgaagcattt
    29581 tggcttgttc ttttccagct tgcaacgtga tcgtgaaata cgtttgtgga cggaactgct
    29641 cgattttctt ttcttggtct ctcactaatg ctagtgtcgg tgtctgcaca cggcctgctg
    29701 ataaattatc cgcgtacttc acagttaagg ctctggtcac attcaatcct accagccagt
    29761 ccgcttttgc ccgggcaaga gcagattcat acaatgcatc atattctttc gcaggtctta
    29821 gttgcttgaa tccagttttg attgctttat ctgtttgaga agagatccat aaccgcttca
    29881 ctggtttatt gaaatgcaca tactctaaga tccagcgagc aacaagttcc ccttctcttc
    29941 cggcatctgt cgcaatgact gcttctgaga catcttttcg ctgagccagc tgcttgattg
    30001 cttttaattg atgtcctgtt ttaggaagtg gtttgattcc tagtttttta gggatcattg
    30061 gcaacgtgtc catttgccag ctctgccatt ccttattcag atcttccggc attttcaatc
    30121 ctaataaatg accaagggcc caagtaacga tcaccttggg tccttcataa taatttttgt
    30181 gcttttgatt agcaccaagc accttgctta ggtctttcgc aacgcttggt ttttccgcta
    30241 gaattagttg tttcataaaa ttcctttctg ctttctactg tgatgggctg atgatttttc
    30301 aacaacgcaa gtcccttgta ttgctgtaaa tcatcgtcac actcttcaca ccagcgctca
    30361 tctccgtcat tccaaaaggc gctgagaaag ttactttttg cattgatata ttcataattt
    30421 tgatggattt cgttccattg tttttcgtac gcttgtcgtg cttcttcgaa agactcgaat
    30481 gtttcttctt cttcaatatc ttcttcccag tcttcaaaaa accaccaagg ctcattgtcc
    30541 ccgaacatgg taattacttg atacatactc gtgctccttc tttcagcttt tctagactca
    30601 tcttagtcac ttttcaagaa agttgcaaga attttctctt ataaaagggt catttaatga
    30661 acatcttaaa attgttacaa atgaatctga ttgtttaagg caaagtagcg ataaagttat
    30721 tgatcatcca aaaatagctg tcttctggat cgattgttgg ttcttttgta aagtatccta
    30781 aaaaacgtaa ggaaagataa ccgtggatca tactacgaag ttccctgctt cgatgaactt
    30841 tttcttcttt cgttaattga aaagcctcaa atatctgcaa aatgatctga ttcgtttcat
    30901 ggattccatc taataaaaca gcttcattgg tatgcggtac actgatcagt aactcataca
    30961 ctgcttgatt tgaaaaagca aaggatcgat aagtttctgc atagatctgc agtgcttcct
    31021 gaccactttt tcccacaaga gcccgtcgca gctggtcatt aagttcctgc agaagtttcg
    31081 ctgttagcgc tgttttgaca tcacgaatat ttttgaaatg gttatacagc gagggagcct
    31141 tgatatccaa gcgtttggct aaatgctgaa aggttatttg ctgaacatcc atctcttctg
    31201 ctagttctcg aaagcagtcg atgattttct cttgagacag atttcttttt ttcatttcca
    31261 tgtttcctct cataaaataa atccttcact ctttcagttt accacgaaaa agaaagagaa
    31321 aaaagagact ttcattgata aactacatat tgtagtttat aataaagagc tgtagatgaa
    31381 aattttcaga aagaaggagt ctaattggct gcaagaataa aggaaaaatt atttaggaaa
    31441 agagaaactt gggaacaaaa cttgtttgtc ttatggtttg gaacatttat ggctggtatc
    31501 ggatttagtt tagtcatgcc ttttatgtca ctttacatag atacgttagg taattatagt
    31561 acgtcacaat tgaacttttg gagcggattg actttttcat ctactttctt ggtgactacg
    31621 cttatctctc cttggtgggg aaaattagcc gaccaaaaag gaagaaaatt gatgctgttg
    31681 cgtgcttcat taggaatggc aatcgtcatt tctttgatgg gttgcgtgac tagtgtgtat
    31741 caattagtcg cattgcgtct gcttcaaggg attttttctg gttatatcag taatgccact
    31801 gcccttgtcg caactgggac gccaaaagaa aaaagtggtc aagttcttgg aaccttagca
    31861 actggttctg ttacaggaac attacttgga ccgcttcttg gcgggatctc tgcttcagcg
    31921 tttggctatc gccctacctt tttcattaca gggatcatct tatttttcgt cttcttgttg
    31981 agtctgtttt tcgtccatga aaaatttgtc ccagtgaaaa aagaaaacat ggtttctgcc
    32041 aaacagattt tcaaagactt gaaatatcca cacgtcgtca tcggtatgtt cgtcacaacg
    32101 atgatcatcc aagcatccaa caattccatt agtccaatca tcagtctgta cattcgccaa
    32161 ttgctgcatg gacatgggaa tgttacgttg atcagcggaa tcattgcttc gatacctggg
    32221 attgccactt tgatagccgc tccaagattt ggtcgcttag gtgacaagat tgggagtgaa
    32281 agaatacttg cgattggttt gggttttgcg attctggttt atatcccaat ggcattcgtc
    32341 acgaatgttt ggcaattagc tgccttacgt tttttgattg gtatctctga tgcttgtctg
    32401 ctccctgctg tccaagcatt gatcacaaaa tattcgcctc atcaagctgc tggacggatc
    32461 ttcagttata accaatcttt ccaagcaact ggtaacgtag tagggcctat gatcggctcc
    32521 agtgtttcca gtgcatttgg ttatcgtggc gtctttcttt cgacttctct tcttgttttg
    32581 gtcaacttct tgcttgtacg acacaataca caagagatcc atcagcaaga aactgcagaa
    32641 gaaaaagctt caggagtctc catagcttca gggcactctc cttctcatag acaataagcg
    32701 taaatgaaaa acactgcttg aacagttgtc acagactgtc cttgcagtgt ttttgtttag
    32761 gcctgttttt tcaagaactc agataagagg aataatccct tttccaaggt ttctttttta
    32821 caacagtaac ctagtcgggc atgcttcggt aaatcaaagg cacttcctgg tactaatagt
    32881 actcctgttt cttctaataa attgatacaa aatgtctggt catcttcttc gatggctaat
    32941 ttaatgaaag aagtagagac gtaattggga gcgacaagtt caactttcgg ttcattggct
    33001 acccattgtt ttaaaatcac aaggttctct gtaatgatct ttttgtttcg cgccaatatt
    33061 ttttctttat tcttcaatgc atgtacagca agatcatctg acaggactcc gccgcaaatc
    33121 atcgtgtagt ctcgatactt tcgaaaaact tctgccaact caggtccagt agctgtccag
    33181 ccaatacgaa tacctggaat ggagtaggtt ttggataggg aattggttgc tatacctttt
    33241 tcatagacat ctacgatcga caagaattca cctttatccg ttaaaggcgc atatacttca
    33301 tctatcaata catatgcatc tactgtacga gcaatttctg cgattttttc taaagttttc
    33361 cgatctagca atgtgcctgt aggattgttt gcactattca aacagatgat ttttgtttca
    33421 ggtttgacta aggcttctag ctgttctaaa tcaaactgcc aatcttcttc ttccttcaaa
    33481 tgaacaaagt caacagcggc acctaaagat ttggggatgt catataattg ctgataagta
    33541 ggaagcatcg aaacaacatg gtctcctgct tccactaacg cgtatagagc taaaagatta
    33601 gcaccagtcg ctccattcgt ttgtaagatg ttttccggat cgacggtttg ataaagagaa
    33661 gcgacttctt gtttgaaaac atctgatcct tcgatccagc catagtccaa tggttcgttt
    33721 ttgcgttttt cgaaaaattt acttacgcta gtcccatcta agccaacgag ttcgtctaat
    33781 gtcaatgctt cgatcgagct ttgtgcaata tcatataccg ctttcttttc ccatttattt
    33841 agccattctt ctacaccgaa atttgcgatt ttcatgtttc tttccccctt atatcttgat
    33901 ccagcctaca gcaagcaacc cgattgaaag cagtgcggcc acacaaatca gcccaatcat
    33961 tactttttct tttcccgtaa tttgatgttt ggtttcacga caagcgatcc agtagaagca
    34021 aaaaccagga agataggcaa tactacataa aaggacataa gaaaatcctg ctaaaatgat
    34081 gcccatcaat tcaaaaacta cagcaatccc accgatcact acttgttttt tgattccttc
    34141 ttttaaagaa aactggactt gatagatccc gacaagtaga taacaaatca aaattgccgc
    34201 tgtacataaa gaataggcaa attgataggc ataatcagta actaagaaag tcaataagaa
    34261 tagattagtc aagattgcag taacaactaa cgaataagtt ggggcatttt ttttattcgt
    34321 ttttccccaa cctttcggca acagaccgtc tcgagccatc aacatcgttg tttctgctgg
    34381 gagcatcgtc caagacaacc aagaaccaat gatcgaaata atcaagccga tattgatcag
    34441 cgctgctccc caatttccca cgattccttt taaaatattt gccatagcag gttgtgaaat
    34501 agttgctaac tcttcttgag ataagacgcc gtatggcaag atcgaagcaa ggatatagat
    34561 aaacaataaa caaagcagac caatgatcga tgcttgctgg gcttctgaac gtttcttcgc
    34621 gcggttggct aaaacacttg cgccttcgat ccctacaaag acccacatca taaccatcaa
    34681 acaaccttta atctgcaacc agatcgttcc ttgctgccct gttccattca ctagattatc
    34741 agatacgtta ccccaaaaat cagcagtgaa tatattcact ttaaatgcga taaatacaaa
    34801 tattaaaaat aggaataatg gcactaattt acagatcgtc acgattgtat tgatgacgct
    34861 cgctccttca actccattgt ttactaataa agtcagaccc caaataaaaa tacttgcaaa
    34921 taaaatagaa ggaacatttt gtccaccttt gaatatcggg aaaaattctc ccatggcgct
    34981 catcagcatc gttgcaaaag caacatttcc cagccacgca gacaaccagt aaccccaacc
    35041 actgatgaag ccacctaatt taccaaatga agcttccgcg taaccgaaaa taccaccatc
    35101 tagatctgga cgcttttctc ctaaatgatt cagcgataat accaaagcta aaataccaat
    35161 cccaacgatt atccatgaaa ggatcgctgg ccctggagct gcagaactag ccagatcact
    35221 tgtgatccca aacacaccgc ttccgattga cgaactgaca accagagcaa ccagtcccca
    35281 tttgctaata cccgtcgatt tcttttccat ttccatcacc ctcacttata aaattgaaca
    35341 aatcacttgc aaaagattcc cttctatttt gcaactgctc ttgcttaaca aaaagcaact
    35401 ttattcatat tattggatat tatgaacttt ttacacaagt atttcaagta taaaatgaat
    35461 aaaaattaat tttattgcaa aaaaacagct atgctatcca aaagaaaagg tgtttctttt
    35521 gttagccata gctgttttga ttcatttaat atcgcttaaa cgtattgtaa aacttttgtt
    35581 tcttgatatg cgtggtcgat caaaccacca cccagacatt ccattccatc ataaaataca
    35641 actgcttgtc caggagtgat tgcacgtaca ggttcgtcga atacgacttc tgcacgatta
    35701 tcatccaata agcgaactgt cacaggaacg tcttgctgac gataacggaa tttcgctgta
    35761 cacttgaatt cttttggcat tggttcattc gtcgtaaagt ggatttcact tgcatccaag
    35821 cttgtggcat acaatgcagg atgatggaac ccttgcccca catataatgt gtttgtcgct
    35881 agatcttttc ctacaacaaa ccagggttct tgcgaatctc ctccgccacc gatccctagt
    35941 ccttgacgtt gaccaatcgt ataatacatc aagccagcat gttgtccttt gacttcacca
    36001 tcaagagtga ccatatttcc tttttttgct ggcagatagt tgctcaaaaa ttgtttgaag
    36061 ttcttttcac caataaagca gattcccgtt gaatcttttt tcttagcagt cgcaaggcct
    36121 gcgcgttcag caattgcccg tacttctgat ttttccatgc cgcctagagg gaacatcgtt
    36181 tttgaaagct gttcttgtga taattggcta aggaagtaag tttgatcttt attattgtct
    36241 actccacgaa gcatgtggac cacgccgttt tcatcgcgag tgacttgcgc ataatgtcca
    36301 gtagctacat aatcggcgcc aagatccatt gcataatcca agaatgcttt gaatttgatt
    36361 tctttgttgc acatcacgtc tgggttaggt gtacgtcctg cgcggtattc ggctaggaaa
    36421 tattcaaata cacgatccca atattctttt tcaaaattca cagagtaata aggaatcccg
    36481 atttgagcag ctactttcgc tacatcctta taatcttccg tggccgtgca tacaccgttt
    36541 tcgtccgtgt catcccaatt tttcatgaat attccgatca cgtcgtatcc ttgttccttt
    36601 aacaaaagtg ctgttacaga tgagtcgaca ccgccgctca tccctacgac aacgcgtgtt
    36661 ttgctgttgt ctgtcatgat atcaccatca tttcaattag attctgaaac atctacgagt
    36721 tgaaggtcgc aacttttcag tattttacat tatacgtcat atttctgaaa atttcatccc
    36781 ttaattatca agtcgtaaaa atactgttct actggaccta ttcagtacac aaacatgata
    36841 gtcaaccaaa aaatcactca tcttttgagc attcgatata aaaaattggc atactcataa
    36901 aaagggccag cctttcggca ataaggaata attttataaa ttgtgaaaaa ctatttttca
    36961 aaattgcttc ttatcgctta aagctgcacg tctcatttac aaccttttct ttttagatgt
    37021 tatgagttgc tcagataagt gattttgttt actctaagaa aaaagggagg caaaagatac
    37081 atgaattact ttatcagtga tatgcacttt ttccatgaac gcttattagg aaaaaacgac
    37141 tttgctccgc ggctctttgc aaatgtggat gaaatgaacc aacagttgat cactagctgg
    37201 aatgcagtaa taaaagaaac cgaccatgtt tatcatctag gcgaccttgc tatgcatccg
    37261 caatatgaaa aagcaaatgc agaaattttg gaactggtca ctcaactgca aggaacaatc
    37321 cacttgatca aagggaacca tgacagtcgc gcttttttcc gttatttaga aaaaaatgat
    37381 cctaggctta ctgacggaag tcccaaattt tatttttatg atgtaggtgc catcgttaaa
    37441 ttcgaccatc aacaatatta cttgactcat tatccgatgt tgctcgggcc taacgacact
    37501 atccgcaatc ttcatggaca tatccatcat tacagcgtac ctatcgagaa cgatgtcaat
    37561 gtaggcgtag acgcaccgga aagagaactc ttaaaggaaa aactaccatt tggcactccg
    37621 ttatctgagc aacaaatcaa agaaatctat gaagcaaaga ggaagtacga taggtcgtga
    37681 aaaaactgcc ctatatcaag tattaaacaa ataaagataa ttactcatat ttttcatttg
    37741 agtaggctta ttgccacagg aacattagga tcagaaatgg agttgtaaca agacttctgt
    37801 cacaactcca ttccatacta ttcgtctttt tgctcatggt gttcttttac cagttttttc
    37861 tgtaattctt tatcataatt tcgggcagct gacgttaaaa ataaaacgat tccgcataat
    37921 aaaatcccga ttccggaaaa caagaacatt ttttctaccc ctaaacgatc agctaatggt
    37981 cctgcaaata ataatccaat tggacctgtc aggttcatca aagagttgaa gttccctaag
    38041 acacgaccta aaacattcga ttcataactc tgctggatca ttgccatcag cagtgtattg
    38101 aaatatggcg ttgcacaacc tgctcctgca ttcaagataa gaaagtacaa gaacccttga
    38161 ctatcgtttg gaaccaatcc gcttgcaccg atcgtcaggc ccataatgat gtaggctata
    38221 ataataggtt tcattcggtc tttccatgtt cctataaaac tgatcagtgc accgcctaag
    38281 agcattccga ctgcgtagac tacttccact gctcccgcct gtccaacgga accaccaaag
    38341 taatctagcg tcatcaatgg atacagactg attgctggca tgaacaacag catcacaaat
    38401 gcaccattga tcgttatgta ccataaccct ttattatcca tcaattgctg cagcccaaat
    38461 tttgcatctt taaaaatagt aagcggaact tccaatattt caggagaaac ttttgggatc
    38521 ttcacaaaaa tcaataagcc gacaccaaaa actgccccca atacatctaa taaaataagg
    38581 taattgacag gaacgactga aaacaaagcc gcacctagag ctggcgcaat gatgaaattc
    38641 gctgattgga ccattcctaa ctgtccattc gtccttgtta tgtgagaaga tggaacgatt
    38701 gtcggcataa tcgactggat cgttggcatt tggaatgttt gtgcgaccga acgcataaac
    38761 aaagacacaa aaactagcca aatcgggaag gattctgaaa tcgttccgac tactgctaaa
    38821 ataagcgcga aaatcgctac aataatatcc gtgacaatca aaagagcttt tttatcccag
    38881 cgatccacta gcggtcctac aaatgggctt agtaaaacca tgggaatcat tcctagtaaa
    38941 gtagcaaaac tcaaaattgt cgccgaaccg gtttctcttg tcaaatacca gatgatcgca
    39001 tattggacga ccatactagt gattcctgac aaaaactgtc ccgataaaaa gaacaaataa
    39061 tttctcttcc aattcgttaa aacgaccgac tgttcatttt ccataaacat ccctcttttc
    39121 tttttcattt tgtatagcgt ttgcacaaca atcattatac gggattcttt tttcaaaaaa
    39181 tacctttttc cctaaataac aatcgtttca atttaaattt ctttacttag aaacaataaa
    39241 tcagcaaaat attaaaaata acaacatgtg ttaaatttaa aaataaaaaa gacaactaat
    39301 ttgcgtcgtc cttacaaagt tagtcatcta acaataggcc gtttcaaaac tagttgtctt
    39361 tttatatcca cagatcattc gactttttcg aataacccac ggctaatcat cccatccatc
    39421 agaaaataaa atttatcttg gctcattttc tgttttggat ctttgaaaat attgactgct
    39481 ttcaatccat ttgctgtagt gatcgacatt ttcaatgttt gaatatcttt tgatacagtg
    39541 atttttaatt tgaatccttc tttgctttgc ggcgtggcat ctaatggtcg gatcaattga
    39601 taattgcctc cgtttgtttc tgtaaatcca ttatcgcgca atgtatatct ttttgcatca
    39661 ggatgcaaac gatagctaat tggtgcgccc agtaccgttg ctgttgtttc aaatgccatg
    39721 attttcacct cgataaaatt agtttctctt tattataaaa gtttatcctg atttttacta
    39781 ggtttttcag ttaagttccc taaaaaatga aggcgttctc tcagacatct gttcatataa
    39841 gaatgacttc actagttatt ttttgacagc acgaactaat tgatcaagga aatactggat
    39901 gtcgtcttcg gtatttccca aaccaaagct gatacggatg gattctttta gtatcggtgt
    39961 atcttctcca tacattgctt ccaatacgtg tgaaggctcg atatttcctg ctgtacaagc
    40021 tgaaccagta gaaatagcga ccccattaag atcaagcttc atcagtagca aatcatttgc
    40081 ttcgccaatt aagcgaagat tcaacacatg agggagttta ttcgttaaat caccatttac
    40141 ttgataaggg atttcgtttg cttctaatgt atcaagaatc aattgctgga atcctttata
    40201 tttgttgtta cgtgcttcac gttcttcagg tgacagcagt tcaactgctt ttgccatacc
    40261 ccagatccct gctaaatttt ctgtacctgc acgccgcttt tcttcctgct ccccgcctaa
    40321 aagcaatgca gggatattta ctccgtctcg tttatataag aaaccgacgc cttttggccc
    40381 attgatttta tgagcagaga tacttaataa atcgattcct aattcttctg gatggatcgt
    40441 ttggtttccg tacgcttgta ccgcatctgt atggaaataa gctggatgat ccgctaaaag
    40501 ttcaccgatt tctttgattg gcatcaaatt tccgatttca ttgttcccgt acatcacaga
    40561 gaccaagatc gtatcttctc gtaacgcttt tttgacctct tcgatcgaca gattcccttt
    40621 ttcatctaca ggcagatatg taacgtcaaa cccctttgtc tctaaatatt tcatggtatt
    40681 aagaacagca ggatgttcga tagctgtagt gattagatgt ctgccttctt tttgacgcga
    40741 gaacgctgtt tctaagatag ctgtattatc cccttctgtt cccccactgt taaagatgat
    40801 ctcgtgaggt tttgcttgca gactgtctgc aatgatttgt cttgctgttt ccaatttttc
    40861 atgcgcgtgg cgaccaaatc catgtatgct tgaaggattt ccaaacgtgt cttgcatgat
    40921 tgccgtcatc gcttcaatta cttgtggatg catgggggta gttgccgcat gatccagata
    40981 aatagttttc acttcattca tcccttccaa attattgcta ggcacattct agcataaaat
    41041 agcgaacaaa ccaaagaaga gctcattttt gtctagagga cgtttcatgg gcatagaaaa
    41101 aaggagctgt gacaaaagtc ttgtcgcagc tccttgaagc tgctcacttc tgtcacaact
    41161 tcttt
//
LOCUS       DARBER010000012        38284 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARBER010000012 DARBER010000000
VERSION     DARBER010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.012"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(165..314)
                     /locus_tag="SCK06_000677"
     CDS             complement(165..314)
                     /locus_tag="SCK06_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150704.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1282..1608
                     /locus_tag="SCK06_000678"
     CDS             1282..1608
                     /locus_tag="SCK06_000678"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1150705.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1589..2524
                     /locus_tag="SCK06_000679"
     CDS             1589..2524
                     /locus_tag="SCK06_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1150706.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3153..3404
                     /locus_tag="SCK06_000680"
     CDS             3153..3404
                     /locus_tag="SCK06_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEG1150707.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3946..4104
                     /locus_tag="SCK06_000681"
     CDS             3946..4104
                     /locus_tag="SCK06_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150708.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4176..5401
                     /locus_tag="SCK06_000682"
                     /pseudo
     CDS             4176..5401
                     /locus_tag="SCK06_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(5490..5882)
                     /gene="rpsI"
                     /locus_tag="SCK06_000683"
     CDS             complement(5490..5882)
                     /gene="rpsI"
                     /locus_tag="SCK06_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1150709.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5898..6341)
                     /gene="rplM"
                     /locus_tag="SCK06_000684"
     CDS             complement(5898..6341)
                     /gene="rplM"
                     /locus_tag="SCK06_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG1150710.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6860..7327)
                     /locus_tag="SCK06_000685"
     CDS             complement(6860..7327)
                     /locus_tag="SCK06_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG1150711.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7447..8985)
                     /locus_tag="SCK06_000686"
     CDS             complement(7447..8985)
                     /locus_tag="SCK06_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG1150712.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9065..9751
                     /locus_tag="SCK06_000687"
     CDS             9065..9751
                     /locus_tag="SCK06_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG1150713.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9952..10479)
                     /gene="msrA"
                     /locus_tag="SCK06_000688"
     CDS             complement(9952..10479)
                     /gene="msrA"
                     /locus_tag="SCK06_000688"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1150714.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10563..11240)
                     /gene="pgmB"
                     /locus_tag="SCK06_000689"
     CDS             complement(10563..11240)
                     /gene="pgmB"
                     /locus_tag="SCK06_000689"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1150715.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11237..12365)
                     /locus_tag="SCK06_000690"
                     /pseudo
     CDS             complement(11237..12365)
                     /locus_tag="SCK06_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12365..14548)
                     /locus_tag="SCK06_000691"
     CDS             complement(12365..14548)
                     /locus_tag="SCK06_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1150716.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14545..15570)
                     /locus_tag="SCK06_000692"
     CDS             complement(14545..15570)
                     /locus_tag="SCK06_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1150717.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15615..16442)
                     /locus_tag="SCK06_000693"
     CDS             complement(15615..16442)
                     /locus_tag="SCK06_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1150718.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16439..17506)
                     /locus_tag="SCK06_000694"
     CDS             complement(16439..17506)
                     /locus_tag="SCK06_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG1150719.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17532..18368)
                     /locus_tag="SCK06_000695"
     CDS             complement(17532..18368)
                     /locus_tag="SCK06_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1150720.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18365..19234)
                     /locus_tag="SCK06_000696"
     CDS             complement(18365..19234)
                     /locus_tag="SCK06_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1150721.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19304..20587)
                     /locus_tag="SCK06_000697"
     CDS             complement(19304..20587)
                     /locus_tag="SCK06_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1150722.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20601..22286)
                     /locus_tag="SCK06_000698"
     CDS             complement(20601..22286)
                     /locus_tag="SCK06_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG1150723.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22561..23586
                     /locus_tag="SCK06_000699"
     CDS             22561..23586
                     /locus_tag="SCK06_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1150724.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24181..27855)
                     /gene="rpoC"
                     /locus_tag="SCK06_000700"
     CDS             complement(24181..27855)
                     /gene="rpoC"
                     /locus_tag="SCK06_000700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG1150725.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(27913..31530)
                     /gene="rpoB"
                     /locus_tag="SCK06_000701"
     CDS             complement(27913..31530)
                     /gene="rpoB"
                     /locus_tag="SCK06_000701"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1150726.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32258..33226
                     /locus_tag="SCK06_000702"
     CDS             32258..33226
                     /locus_tag="SCK06_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1150727.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33244..34149
                     /locus_tag="SCK06_000703"
     CDS             33244..34149
                     /locus_tag="SCK06_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150728.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34146..34952
                     /locus_tag="SCK06_000704"
     CDS             34146..34952
                     /locus_tag="SCK06_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1150729.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            35088..36077
                     /locus_tag="SCK06_000705"
     CDS             35088..36077
                     /locus_tag="SCK06_000705"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG1150730.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36394..36918)
                     /locus_tag="SCK06_000706"
     CDS             complement(36394..36918)
                     /locus_tag="SCK06_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG1150731.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            37205..>38284
                     /gene="scm"
                     /locus_tag="SCK06_000707"
     CDS             37205..>38284
                     /gene="scm"
                     /locus_tag="SCK06_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1150732.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDS"
BASE COUNT        11049 a         8062 c         7108 g        12065 t
ORIGIN      
        1 aacaatcttt ttattaaaaa gaaacattat aatatgaatg tttgtgaaaa aataatacaa
       61 cttagtcatt ctttacaacg atttatagaa aaacaacctc tttaaatata aaaggtacgg
      121 gttactgatt acttgtaaat ctttacgttc cagttttatt ctttctaaaa aatatactca
      181 cttcttttct tagacttctt tttttgattc tcctctttat ggtcttgtcc ttttttgcct
      241 tcaaaatatt taaataaacc cctttcaaaa gcatttctag gttttggaca ataaggaatg
      301 acttttttct tcatataaat acctcattta actatttgat atcatcacat tatcaaaaaa
      361 aataggtttt ttattaaaac tatcttgacc tttcgggaga tttcactgtg atttattttt
      421 attcccatac ttttttcgtt ggtcactttc gtatacctaa tctaagcgat catcggcgac
      481 ggtgctttta caacgaatgg caggacaaaa caaggggcgg aaaaactttt ttgcctattt
      541 cataaaaaac tttttcctac agcaatccct tgttttgtcc taccacacgt tgtgaaccca
      601 aagcacgccg acgaacgctt agctatacga aaatgacctc caaaaacaaa aaagtgaggt
      661 gaatagaaac aaaaagaaat tgaaatctcc caaaatgtcg tccgcctcgg agacaacttg
      721 ctgctgaaaa tggttggtta attcatttaa gtattaaata agataaatca tcaaattaat
      781 cggaaaaata gcatgattgg ccttatcaaa aataaactgt aaaaacttat atttctatga
      841 gtggtggaac aatgctaata ccaaattaat ttatcgatta cccacctgca caaaccaaag
      901 atagcattat taggaaacga atcaatcagc gaatccagca ttataatgag gatactaaaa
      961 ataaagtttt cataattata aatccgcctt cctacgaaag gcaccaatga ggtgatgaaa
     1021 accttagagc gctttctttc tttttttatt ccgtgttacc ctttccttgc aggtccctgg
     1081 tacactacag aacccgagga ggtgagtggc ggggctgtat agcggcaacc ccgcataatt
     1141 atatggtgtc ggtcatccgt taaggcatca atgaatggcg caaacccgta gcccgtaaac
     1201 ttatctcttc cgaagttgac tcgatttcgc cggatgacca gtccctacca aacctgtact
     1261 atttttttac agaaggttgc tatgttcaaa atctttcgta aaaattgttg tggcttggat
     1321 gtccacaaaa cgtggatcta tgcgtgtatc ggaatcacag atgcgaatgg tcgtacagtg
     1381 tataagcaag ctcgcttctc ttcctttaca aaaggtctca atgaattagc tgaatggctt
     1441 gctaaataca actgtaagga tgtgtgtttg gaatcttccg gtaaattttg gattcctgtt
     1501 tttaatattc ttgaaaaaac ttgttttgtc acactggctc atccgaagta caccaaaccc
     1561 cagaaaggaa ataaaacaga tcgcaaagat gcaaaaatgg atttgtgatc tctttatgtg
     1621 cgacatgatc aaacccagtt ttattcctgg tcttgaaatc cgccagcttc gtgatctaat
     1681 gcgttatcgg acgaaactga ccaacatgaa aacaagtgaa aagaatcgtg ctttaaactg
     1741 cttaaccgtt tctaatctga aactagatga tgtttttagt gatgtgttcg gaaaatcatc
     1801 cacttctatc atccaatact tgttagaaca tcccggtgaa tcatttgatg ttcgaccatt
     1861 tatccatgcc cgttgtaaaa ctcccgttga agaaatccaa gcggcagttg agggggcgat
     1921 ttctgtacct caagcgatta agcttcgcca atgcctcaac catattaacg agattgagaa
     1981 gcatcgagaa gagatcaatc tagagattca agctcttgca gaacccttct cccctgtttt
     2041 ggaactactt cgtaccatac cgggcttaga cactcagcca ataacagcga ttgcgattct
     2101 ttcagaaata ggaccggata tgtccgtgtt tccaacttcg aaacacttta tttcttgggc
     2161 tggatgctgt ccaagaaacg atcgcagtgc ccgacgaaca aaatcaacac gtatctctcg
     2221 tgcagggcag tcgttgaaac cgctacttgt acagattgcc aatgctcttc tcagatccaa
     2281 agaacatcca gaatttaaaa atcgctatcg tagaatcaaa gcgaatcgtg gacacaagaa
     2341 agctatcatt gctatttgta agatgctcct gaccgctatc tggaatgtct tatcaaaggt
     2401 ggaaccctat tctgctaaag gatatctgga gccaaaacct gcgaaagaag agaaagttct
     2461 gacgcaatcc caagctctgg accttttaag aaaacgcgga tataccatta tagatagtgc
     2521 ttaaaccaaa gttacgttga ttcttcctaa atagactcgt tgtcttccat ttcaaaaatt
     2581 ttgaaaacgg taggcttatt tagtgtaccg ttcttcaggt atcttcaaat agatttattt
     2641 tcaaacttat ttcctttctt cttttactct cctatagctt agcatttaag tgggcactcg
     2701 tgtcaccttg ctagatagct atgaatgccg taataagcct cctagtatat aggcagactg
     2761 ccaattataa ctgattcaat ccacattatg ataaaatcca ccttttcgtt tcatatgtgt
     2821 tgaaatatca catatgtcaa aagtgcacga gtcttttcat tcagtgcttt gtaaatactc
     2881 aaaattattc cttttcactt taaaaaaaag ataattagta caaaatcaga aatgaggaaa
     2941 gtattaggat ggactagaaa ggagttgttg aatttgttgc aacagattgg tcatttatca
     3001 attaaaaatt aggtgaagtt ttattcatca ctgcagctgc tgtttcccag agaataaaac
     3061 gtctagaaga aactggtcag ttactgtatt atcaagcagc aattgactac gaacaagctt
     3121 gattactaac caaagcgttt atccacctgt tgattaaatc aaatcaaaaa tcaggttttt
     3181 atacgttcat aaaagcaatc tccagtgtac tcgaatgtga ctgtatcata ggtccttatt
     3241 cgatgttaat caaaatgggg tttccttcta ccgaatcatt ggacagtttt atcaatgatt
     3301 tatcaaagtt tggacgaacc aacacccaaa tcgttttctc gactcttgtt tccttaatgg
     3361 tttatcagtt ctggaatgtt tatcatgcgc ttttattaaa atagaaaaga gcttttttca
     3421 tgcgacaaag cagtgatcat ttattggcac atgcaatctt tgtggatcca ttttttgaaa
     3481 atacgatatt tagttttgac ccaaaaatct ttaaatacgt atttgataat ttatcttaac
     3541 aaccagataa ttttattcag caccgtatta tctatattga attctcggaa tcaagataaa
     3601 tttttttccg tttttgtttc ttttttaatc ttcttcttgt tattaatagt gattaggatg
     3661 atattcctat attgatcttt gataattaga tataattttt gaataacgtt ccctgctatt
     3721 cgagctagaa atacaagtac gccatgacct agttagcgac aactacttag agatttcttt
     3781 tgttctggga caacaaaatg ccatgacaat agcagtaaat aatgatactt ccgtaaggct
     3841 cgccgtaaac tggggagagg tgaaattcct atgtgcttac ccaaagcggt tcaaaaattt
     3901 taaactatta ttttaggaga tgattttata aaatcgaaag cctacttgaa taaaaaacaa
     3961 gcctgtgaat ttctaggaat tgataataat actttagata agtggattcg tgtatataat
     4021 tttccagtaa taaaaattga tggcgtagtt cgctttgacg tagaggatat caaagatttc
     4081 atgaatacat tcaaaaaaaa ttgaaatttt cttctcgtca catatactat aaatagcgaa
     4141 atgtactgcc ttacttttta aaggagtgag gaagtttgcc tacaaagctt tcaaatggga
     4201 aatataaaac taatctgagg tatccaaaaa aattcagaga aatcactgga attgcatcag
     4261 agaagtatca aaaagtattt ccaactaggc aattagctat taaggccgaa agggaaatga
     4321 aaaagaagat tgaaacagtt ttaagagagg agaatgcaaa ctcactagaa ttgaaaggaa
     4381 aaataaaatt taaagatttt tatgaatcaa aatggcttcc tagatatgaa ctaggtcaaa
     4441 ctactaggtc aaacagggct ccaagtaatg taacaattag taatactaag gatatattcc
     4501 gattgcacat ccttccaatg tttggtgaat atgcaatgaa ttatctgaat tcaaatactg
     4561 agataatatc agatgaatta acaaagaaat cgaaagaata cgcaaatata aaaattatca
     4621 aaggctatgt tcgctctatg tttgatattg ccgaaatact atgttatatc gaattcaacc
     4681 gaactaccaa aattattcaa tgtattaccg cgcctaagaa aatggcttta gaagaaaaaa
     4741 ggataaggga agggaatcaa gcattatcat cagaagagct gactgattgg atagaagcag
     4801 taaacaatga cttaaataat cacttgctta cacttcacga ttacacactt ttcatgctta
     4861 ccttatatct aggtgataga aaaagtgaga cttatgctct gcaatggaaa tatattgatt
     4921 ttaataaaaa tacggttcgt ctcaaatatg ctttagataa atatcaaaga aagacacata
     4981 caaaaggttg gaaagacacc gttattcagg caccagaagt tgtgatgaca ttattaagag
     5041 aatggaaatc agctcaggct gaacaattgt tgaaactaaa aattatacaa acacctgacc
     5101 agtacctctt tacctattcc aaaccttctg gagaagtaaa ttgtcctgtt cacgctgact
     5161 acttaaacta tcgaatcaat gcaataaaaa aaggcatcct gaattggctc aattatctcc
     5221 acacaaactt cgtcatacct atgcaactat agctagggaa ggtggagcaa atatgaatca
     5281 aatctctaat gctttaacgc actcggatat ttctactaca aaaatatacg tgaacacacc
     5341 tgacgtagtt gataaatctg tttttgaagc atttcaaaaa ggattgaata agtgtgatta
     5401 acatgtgttc tcaaatgtgt tcttattata aaaaaagaca ctttccaaat aattgaaaag
     5461 tgtcctgaaa tattgatata aaaagaatat taacgttttg agaattgtga agctttacga
     5521 gcttttttaa gacctggttt tttacgttcg accatacgtg cgtcacgagt aagtaagcca
     5581 gcacgtttaa gagctaaacg gaagtcagga tcaacttcta gcaatgcacg agcgatacca
     5641 tgacggatag ctcctgattg tccagcgtat cctccgccgt ttacgttaac aaatacgtca
     5701 tatgcgcctt ttgtttcagt aacagcgaaa ggttgattga ttacttcacg caagtcagca
     5761 tgtggaatgt attcttcaac atctttttta tttacagtaa ttttgccagt tcctggtact
     5821 agacgtacgc gagcaactgc gtttttacga cggcctgtgc cgatatattg aacttgtgcc
     5881 aatgaaattc cctcctatta gattaagttt gtgatgtcta aaacttctgg ttgttgtgct
     5941 gcatgtggat gttctgctcc accgtatacg ttcaattttg taaattgttt gcggcctaaa
     6001 gtgtttttag gaagcatacc ttttacagaa gtttcgatca aacgacgaga atttttagca
     6061 cgcaattcgc cagcagtgat tgatttcaat cctcctgggt agttgctgtg acggtaataa
     6121 attttgtcag tcgctttttt acctgttaat ttcactttgt ctgcattgat gacgatgacg
     6181 aaatcgccag tatccaaatg aggtgtgaaa gttggtttat ttttaccacg tagtacagat
     6241 gcaacaactg ttgaaagacg tcccaatgga acatcagttg cgtctactac ataccattta
     6301 cgttctactt cgcctggttt ggccatatat gttgtacgca cgttattttc ctccaattta
     6361 agttctgatg tttgacatac acttcgagtt tccggggctc tttgtggggc aaacaatacc
     6421 atttaacata ttacccgcta atctagtcaa tgtcaacgtt tttctgcttg attgaaaaat
     6481 ttatatgaaa ctttacttct tttctttctt ttatataaaa gggtcgtttc ctgtcaaaat
     6541 gaaaaacaaa aagaggctgt gacaaaagtc ttgtcacaac cccttatttt gaataaacgg
     6601 tgttctcctg ccaactcctc ggtcacaagt cacaatatcc ataaaacata agaagctgtt
     6661 ttttaaaatt gtttcttgtg actcggagct gaacggcagg cttacaacct cttggtaaac
     6721 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     6781 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctttttcgg
     6841 tcaagttcaa caacagtttt tacgcgtcga tctctggcgc tttgcccaaa tgagcttgga
     6901 tcatccagat acgtttttct gtggctgttt tgaattcgat aaagatatct tgtgttggat
     6961 cgtcgccttc ttctccagaa acttcgattc ctttttggta aagatctgct agataacgat
     7021 agcctgcaac aagtgtttcc aaacgttctg gaatagtacg atcccagcgg ccaatttcat
     7081 cagggatttt tgtgttatct gcaaattcac gcaatgtaga ataaggtgaa ccgtctaatg
     7141 tgatcaagcg ttcggatact tcatctaact gagcgttcaa atcatccatg aactcatcca
     7201 tcattgggtg caatgtaagg aaacctggtc ctctcatgta ccaatgtgtt tggtgaacaa
     7261 caacagacat ttggcttaga tctgcaacta attgatttaa tacttctttg gtttgttgga
     7321 atttcatctt tcttgcctcc taacattttc ttacacttaa aataatagta gactttcaat
     7381 gaaaagtaca acaaaatcat ttacctgaga acgattttca gttcgctttt ttatccgtaa
     7441 agccaatcat ttttcagaat cagctaattc ttctcgcagt tgagcaaatt gctgataagt
     7501 ctgtgctaaa tgcttttgtg tcgaaacaaa taatggatag attttctgat attttttatg
     7561 atcagtttct tgtactccaa cttgctcttt caattctatc aattcctgtg cttcactgat
     7621 actttggatc tttccgatgc ttctccagcc taaaatcaca gctcctaatg cggatgcttc
     7681 tgtgacagta gggaaaacaa gcgggtaacc cagtacatca gccatcatct gacgaaaaat
     7741 cggagattga gcaaacccgc ctgtagcacg gatctctgtg agctgatttt cctggcaaac
     7801 tccttttaaa atccgataaa ggttgagaca gatcccttct agaatcgcct tcatcatttc
     7861 tttttctgta tgttgccgct gtaaaccgat aaaactgcca aacgcatctg cgtcccaaag
     7921 cggtgctcgc tcaccaagta aatagggatg aaaaatcaga ccattggcac ccgctggcac
     7981 aaaagcgata gccgaaagca gtgcatcata tggattttct cctttttctt tagcagattg
     8041 aacgacttcc tgtaaaaatg tatgccctgc ccaatcaaag atgccagcac cattgctagt
     8101 cgctcctccc actacccaat gtttctgatc cagaacataa caaaacgtct ccatttctgg
     8161 atggaactga ggctgagtag taataaaacg aagtgctccg ctcgttccta cggtcaaagc
     8221 ggcgatcgta tcaccaaaag cacctaaccc tagattagaa agcggcccgt ctgcccctcc
     8281 caaaacaaat gatatatcat ttggcaaacc tagttctgct gctgtttttg atggtagacc
     8341 tgttacttga taggtggaag gaaagacttg cggcaattgc tctattcgaa tgttcaagta
     8401 gtcaagcact tcttctgccc attggaattc atggatatca aaatatccag ttcctgaagc
     8461 tgttccgtag tcgctgatca gttcgccaaa caatctataa aagatatagt ctttgatacc
     8521 gacaaaataa gcagtttttt tgaataattc aggttggctg ttttgcagcc acttgatttt
     8581 gttcaatggt gtcattggat gaatcggcgt cccagtctgc cgataaaatt ctttgccaag
     8641 cacggtatat ttcagttttt ctgcttcgtc cgctgctcgg ttatctgccc aagtgatcat
     8701 tcgagataac ggtgttttct cttcatccaa caatatcagg ccatgcatcg cagaggagaa
     8761 ggagacaaat tgaatgattg tgtctgaagg caactgttta attacttctt ttataacggt
     8821 gcagacacct tgaaagatca attcctgatc ttgttctgcc atcccttgtg tttcttggat
     8881 caaagggtag cgaaaataag cggatgctgc ttcttgggct tcatctgtga aagcaactgc
     8941 tttggtttgt gtggttccga tatctaatcc gattgaaacg ttcatttttt cacctcaaat
     9001 tttatgtatg tacaaaaatt tagtttaagc ttgcgtatct tggttttgaa aagggggaga
     9061 tggcgtgtta ctttatttta tcattggttg ctgtatcgga tcatttttat gtttaaccgc
     9121 ccagcggatt ccgttaggtc actctattat ttatcctaga tctcattgtg tcaaatgctg
     9181 tcgttcgctt tcttggtatg aattgattcc gattctctcg atcatcgtcc aacgatttcg
     9241 ctgccgctat tgcaggtgtc ggttgccctt ctactatttg ttggcagaag ccttatgtgg
     9301 tggtcttttt gcctggttct ttactttttc ttccgcaaga aatttttcga cgttcatatg
     9361 gatgctttcg gctttgctat tttcgttgat ggatttattt tacttcatgg tccattctca
     9421 cacgctattc tgttcttggg caattttatg gattttttgg ttgcaggccg gcgtcttcca
     9481 atggcaaagt gtgctgttaa cgtttatcgt aggcggtgtg tgccttcgct atggtcaatc
     9541 ctatctcgga gctggcgata ctctgttgat gctttcttgg agtggtggct tgtctctaga
     9601 agagttgctt cagatcttgt ttcttgctag tctgtttgga atatccgttt tttctgttta
     9661 ctttatctgt cataaaaaaa agttggaaaa gctgcctttc attccttttt tgagcagtgc
     9721 gttgctgatt gtccttcatc ttaggtattg aaaaaaggtt gtgacaaacg acttgtcaca
     9781 acctctgata tatagtgttc tcttaccaaa tccttgtgac ttggagctag gcgacaggtt
     9841 cacaacctct ccactaaatg gtgttcaatc agctgaccct cagtattagc tcaaaatgct
     9901 ggttttacaa cctctgatat acggtgttcc ataagtagct ttttcggctt cttatttctt
     9961 gacgccccaa tgtcgacgaa ggaaacctgc acgtccagaa ccttctcgat aaagattgta
    10021 atggagcttg tttttcttgt aataatcctg atggtaatct tctgcaggat aaaattcgct
    10081 tcttggttcg atttttgtca cgattggctt atcgaatcgc ccgctgtctt gcaatgcttg
    10141 ttttgaacgc tctgcaatat ttcgttcttc ttcatcatta tagtagatca ctggacgata
    10201 agaatcaccg cgatcagcga actgtcctaa tgcgtcagtc ggatcggttt gctgccaata
    10261 gatttccacc aactgatcat aagaaataac ttctggatca tattcgatct ctactgcttc
    10321 tgtatgccct gttgtgcctg ttgttacttg ttcataagtt ggatttggta catgcccgcc
    10381 agtataaccg gatgtcacag aaatgatccc tggctgctct tcaaatggtt tgaccataca
    10441 ccaaaagcat ccgcctgcga aaactgcttt ttttgtcata taaaatcccc cttttttctc
    10501 atcatacctt gatttccaaa aggaggataa cgttttgctt gtgacagaaa aaacggaaac
    10561 tattatatac gtgatacggc ttctgataaa tgccaaaacg aacgtacttg atcaaaaacg
    10621 gttaaatcag gaatcacata ttcagcagaa ggaagattta tgttttcacc tattcccaat
    10681 acatgcatgt taccagccaa agctgcttga caaccagctt ctgaatcttc aatgaccaag
    10741 catgcatttg gcggtacatt caattgctgt gctcctttga gaaaaacttc tggatcaggt
    10801 tttgcttttg atacttgcgt gccgtcaata aggacatcaa aataacttgt caaattcgtt
    10861 ttttctaaaa tgaggcgtgc atttttgctt gctgatccca atccgatctt gatgtttgtt
    10921 tttttcaagt actcaagaac agctattgca ccaggcaaaa cggatgtttc atccattttt
    10981 tgaatcgctt gaagatatag attgtttttt tgctcagcaa aggctgattt ctctttttct
    11041 gagaatttct gttccatttt tccaaatgac agcaatcgct ctagtgactc ggttctggat
    11101 attcctttca gttgctcgtt ttctttctca gtgaatggaa tggacagctg gttagctaaa
    11161 accagccacg cttcgtaatg ataacgagct gtgtctacaa gaacgccatc taaatcgaat
    11221 agtgctgctt tgattttcat cgattacctc cttgaaaatg aacgacttga agtaaagatg
    11281 cgatgttttt agctgtgcga tgaagattat cagtggcttg ctgtaaagct tcttgtaaag
    11341 gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc aacatctgct gctttttcgc
    11401 ttcctttacc aacacttccg caaatagcta tcacaggaac gccctctggt actcttctag
    11461 caatcccaac tggcgctttt ccttttagac tttgtccatc tagacgtcct tcgcctacga
    11521 ttacgaggtc tgcttgctgt acccgtatcg aactccagta aatccaatac gtagtcgatg
    11581 ccagagacta ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca
    11641 ctactgcccg atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct
    11701 tggtcagcca ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa
    11761 gcgccttctt ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta
    11821 atcagctcaa taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc
    11881 agtgcttcta ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg
    11941 ccgatgccgc catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct
    12001 tgttgcaaca attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt
    12061 tgttggttta gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt
    12121 tgaccgtctg aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct
    12181 gtttgccgct tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg
    12241 cctataggta gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg
    12301 gcttgtgctg cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata
    12361 cgcattattt tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat
    12421 tgacaaaaat aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca
    12481 aaacaaatag caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg
    12541 aaggaagtct tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa
    12601 atataactgc tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat
    12661 gcggatctgg tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca
    12721 tttcttggat acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga
    12781 ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag
    12841 gtaataagta taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt
    12901 cgctacgggc ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca
    12961 aatcaataac tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag
    13021 gcactggcaa atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc
    13081 cagcttgata tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat
    13141 tattgtcgat atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat
    13201 catcgacttt tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc
    13261 cctttgtttc taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag
    13321 caaggtctgc actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg
    13381 ttttgatatt caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc
    13441 atgggaaaag tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc
    13501 gatcataacg gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag
    13561 gcaggataaa aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc
    13621 ccttcgcccc tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag
    13681 cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct
    13741 gatgccaaaa atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc
    13801 ttttctgggc ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga
    13861 gcacgatgat cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag
    13921 cggtgatccg gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc
    13981 caatatcaat tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata
    14041 ctcgcagttc tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac
    14101 ccgttttcac atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg
    14161 cctgatggag attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt
    14221 gagcagtttc aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct
    14281 cgtatgcaag aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc
    14341 atctgatgac atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat
    14401 atacaccagc tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc
    14461 tgataccaaa aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta
    14521 aatcaaactg tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg
    14581 atataaattt tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg
    14641 agtctggtca ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt
    14701 aactggcgtg gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc
    14761 tggatacaga ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct
    14821 gatattcaaa gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt
    14881 gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga
    14941 attttttttc gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa
    15001 tccagtaata tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc
    15061 tccttgaagt tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc
    15121 attcgcttct acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc
    15181 ttcgtcagaa aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt
    15241 ctctgccatc tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga
    15301 agcattagct tcgagggcag ccaatacatg ttcgaagtga aagcgattgg ctgtacagac
    15361 actcacgata tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga
    15421 aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc
    15481 caattctaag acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg
    15541 tccagcgcct atgattgcta ctttcttcat ttatttctct ctcctttgct tatttactta
    15601 ttgttattct ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag
    15661 tttttcaagt attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga
    15721 cattcatata ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta
    15781 aaatcaattg cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc
    15841 acatactctt tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga
    15901 taaaaatcag cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca
    15961 tccgctaaca aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc
    16021 gtacctgttt cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag
    16081 tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa
    16141 gctgctggga taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca
    16201 atttgacgta caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag
    16261 ccgttacaag ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg
    16321 atattttcta ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata
    16381 aatctaaatt tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc
    16441 attgttctcc tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta
    16501 cataacgcat gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt
    16561 cacaatccaa gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt
    16621 tccatcttgg gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat
    16681 tattgaaatg tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa
    16741 cataagaaat gatcccgcca tagccgatcc ctcttaaaga agcttgtaat gcggcagcac
    16801 tgccactcgt ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa
    16861 aaccgacatc tgctttcata ggatctagcg tgtaatcggc accgtatttt tctgctaatt
    16921 ctctacgctg cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta
    16981 attgaatcgc aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc
    17041 gaacatgtgc ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat
    17101 ttttccattg atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa
    17161 tcagcgtttc acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca
    17221 ccaaactgcc cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa
    17281 ataccacacc gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa
    17341 attcttcatc gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg
    17401 gagtagcgaa aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat
    17461 ctttgaatcc ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct
    17521 ttcttttctt tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca
    17581 gataacgcat acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt
    17641 ccccaaatgt agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt
    17701 tcggaggtcg acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa
    17761 taaacgaaga cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc
    17821 tggatccggc taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca
    17881 aaataacttt ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc
    17941 agtgctgtat tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg
    18001 atcaaaatac cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct
    18061 ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg
    18121 gaggctatca aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca
    18181 gcattgaaaa tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc
    18241 gttgcaattg cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac
    18301 aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta
    18361 tttttcattt caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga
    18421 acaatagaat cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat
    18481 acaaataaac gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat
    18541 agaacatcca gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca
    18601 gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa
    18661 gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc
    18721 tccagatagc aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg
    18781 gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat
    18841 tattgaacat atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata
    18901 aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg
    18961 ctaatcctaa agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta
    19021 aagaacgata aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa
    19081 cggcgtctga gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca
    19141 ttggaatcag cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta
    19201 attttaagtc agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca
    19261 atagctatct acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt
    19321 gtgcgtcagc taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt
    19381 ttccgatcac acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta
    19441 gtccaaagac ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag
    19501 acttgatgac ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt
    19561 gagcaccacc aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag
    19621 ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc
    19681 cagatactgt gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt
    19741 ctgagttccc cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg
    19801 ctgttccatt catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt
    19861 attgggataa gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta
    19921 cctcgccttc atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt
    19981 gctcactcat tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat
    20041 ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag
    20101 cttctgataa tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag
    20161 caatatagct ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat
    20221 cttctccagc ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat
    20281 ccatcacttt agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag
    20341 ctaacgtaac gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga
    20401 tgtctggatt ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct
    20461 gttcgacaga agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac
    20521 tttcaccaca agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt
    20581 ttttcatttt tttccacctc ttattttatt attttcagcc acatatattc atacggctgg
    20641 aatatcaact ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa
    20701 tctccttttg gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact
    20761 ttctcttcct tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa
    20821 atcaaactat tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat
    20881 tcaccatata ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac
    20941 aaaaattttt gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca
    21001 tttcgacttc ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa
    21061 aatgaatgag cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt
    21121 aacgcatcta gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc
    21181 ttgtagttta ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt
    21241 tccggcagaa tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta
    21301 aaaaaagttg tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt
    21361 gtattacccg aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc
    21421 tgtgcttcat tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc
    21481 tcagtgatca ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg
    21541 attttatgcg tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc
    21601 acagcgtcca agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc
    21661 attttcaata aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc
    21721 gtccatacgt gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc
    21781 cttggtctag tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg
    21841 tacgtatcat tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg
    21901 cagacaaagt cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg
    21961 cccaaacggt cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat
    22021 gggaaaaatg gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat
    22081 ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc
    22141 aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa
    22201 acactgactt tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa
    22261 atcgcttgta aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa
    22321 cgatttgatc atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga
    22381 tatacacaaa aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa
    22441 atatggtctc tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag
    22501 aatgtctatt gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa
    22561 ttgaaaccta ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc
    22621 agagtattaa ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca
    22681 atcaaagaat tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt
    22741 gacgcgattc tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt
    22801 tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta
    22861 caaacggcaa aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa
    22921 atgatccgag gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg
    22981 gatcaataca atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc
    23041 tactcagtgg acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag
    23101 aatggacacc gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat
    23161 cgtctcaatg gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg
    23221 attatagacg gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag
    23281 ctacgagaaa gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac
    23341 aaagtggcag aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt
    23401 gatcccttat tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat
    23461 cagttaggaa tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa
    23521 gaaaaaagaa tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa
    23581 atgtagaaca gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt
    23641 ttttcaatca ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt
    23701 ccatctgttt tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca
    23761 gggaagaaaa aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa
    23821 acacatttac ccttatttca atccatgtta tttggggata cttatctcta aaacataaat
    23881 agaataacag acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag
    23941 caaaatatta aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga
    24001 tctatcttta cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt
    24061 ggatacgaac cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt
    24121 ttagacaaac caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat
    24181 ctattttgtt tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat
    24241 actgtagaca ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg
    24301 cataccagta ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag
    24361 aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga
    24421 tgcagcagat aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg
    24481 tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc
    24541 tgcgatgtct aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac
    24601 tttacgcagc atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg
    24661 catacggtaa acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg
    24721 aacttgcaac aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat
    24781 catatcgcct tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc
    24841 tgtttttcct ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat
    24901 gacatcccct gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat
    24961 ttcttggaca cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa
    25021 tgtacgcatc gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac
    25081 tgcttctccg acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca
    25141 gacaccatgt tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc
    25201 atcaacgatt tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc
    25261 atttgtttca ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa
    25321 tgattcgatg atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc
    25381 gcaatcttct tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta
    25441 acctgaatcg gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga
    25501 gataaacatt tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat
    25561 gattcgacca ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga
    25621 aatgttacca cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc
    25681 ttccatcagt ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc
    25741 ataacgttcg tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg
    25801 tttatgggca ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc
    25861 tactgtgata ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat
    25921 cttagaagtt tcttttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt
    25981 tttcttcttg aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc
    26041 agcttcaaca aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt
    26101 cagataaggg aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat
    26161 catcgtacgt tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac
    26221 accgatacgt gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg
    26281 atctctgaag accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt
    26341 acctagaacc atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat
    26401 gttttgagca gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac
    26461 gtggacagcc atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg
    26521 caaacggatt gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg
    26581 aagcgtaggt gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc
    26641 ccatacttcg tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag
    26701 ttcgcgttga acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt
    26761 tggaagtcca cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga
    26821 gtagtcgaca cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat
    26881 atgagaaaga gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc
    26941 gttatcgatc aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat
    27001 tccaggtgca ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg
    27061 gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg
    27121 taaatctggt ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga
    27181 agcgcggaat gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg
    27241 tgcagatttc aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag
    27301 taattgttta atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg
    27361 tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg
    27421 ttcgatgact acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc
    27481 taatacaaga cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc
    27541 tgctaattca atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc
    27601 gcatcgatca caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc
    27661 ccagtctttt gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa
    27721 ggtacgatag ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt
    27781 ttcgggagaa gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg
    27841 ttccctccat ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc
    27901 tcatagaccg gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc
    27961 tttttcatct tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc
    28021 ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg
    28081 aagttcgatc tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa
    28141 ttctttcacc aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt
    28201 gacgattgct tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat
    28261 ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa
    28321 acgttgtcca ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg
    28381 tccgattgaa cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat
    28441 cacaccgaca gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt
    28501 tttcgcatcg ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc
    28561 accatcaaat actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg
    28621 taattccaat acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat
    28681 atcgattggt gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac
    28741 aaccccttta tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg
    28801 aacgatataa acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg
    28861 agtaaagatt ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt
    28921 atcgcggact tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga
    28981 taattctgtt accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc
    29041 tgcaccaata cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt
    29101 agggatttcg cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata
    29161 ttcttcaata tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat
    29221 cgcatcttca tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag
    29281 agccatttcg ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt
    29341 ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa
    29401 ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc
    29461 atcaacgtat tcaacgacac catcatgttt acataacaaa gcagctcctg agtcatgcgc
    29521 tgatttgtat tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc
    29581 ttgacgctgc atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg
    29641 aatacatgct gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt
    29701 atcaatagat acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa
    29761 tgttccatct tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc
    29821 agctgtcaag tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata
    29881 aggtgtttcg ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa
    29941 cccgatgttt gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg
    30001 aacgtcacga acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa
    30061 aagacgacgt ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa
    30121 ctgagaagaa ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg
    30181 ttgaggtgtc aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat
    30241 acgtgctagc ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg
    30301 atttcctaag tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata
    30361 gctcattgaa gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc
    30421 gtttccgatc acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg
    30481 gacagtcatt ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag
    30541 gccattatca atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt
    30601 ttcaacgatg atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa
    30661 tagacgtgtt ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc
    30721 gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt
    30781 tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc
    30841 gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa
    30901 gatttcaaag atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg
    30961 aatcttacgt gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc
    31021 tagccaagct ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc
    31081 gacttttcca tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc
    31141 tgccccgttg atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac
    31201 ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg
    31261 tgcagagtag tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa
    31321 ttcataatct acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc
    31381 ttcaaacatt tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc
    31441 gatcaaattt ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg
    31501 ttttccgtat tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa
    31561 aagactaccc gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga
    31621 cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta
    31681 cttcgtgagc ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt
    31741 ctgccttttc ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa
    31801 agtcaacact tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt
    31861 ttatgttttc aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt
    31921 tagaaaattc attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa
    31981 cacaactgaa taacttttac tttagctact ttagataggt ttgtattcat catgatggat
    32041 acaaacgtta aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa
    32101 agccacgtag acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag
    32161 cattctactt tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat
    32221 ggattttttg tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg
    32281 attaggcgct gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga
    32341 ggcatcagat ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga
    32401 tgctgcctat aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa
    32461 tctaacaatc gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga
    32521 gaaactcgtc aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc
    32581 tttattaaat gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca
    32641 agcccattta gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat
    32701 ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat
    32761 cggcatcatg tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa
    32821 agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt
    32881 ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa
    32941 cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt
    33001 cgttgctggc gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta
    33061 cgaaagcttg ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc
    33121 aagtgaactt cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc
    33181 caaagcatta ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt
    33241 ctcatgacaa atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc
    33301 ctacttgcga ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct
    33361 gaaggaagct ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat
    33421 ccattgacag cttgtcttct aggcttttta gggagtgcgg cagcagggct tgtttccgga
    33481 ctgcttcaca caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt
    33541 ttatattctg tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca
    33601 acgatctttc attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc
    33661 ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca
    33721 ggacttgcta ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac
    33781 acagataata tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc
    33841 tccttgcttg cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta
    33901 attggacttg cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc
    33961 cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc
    34021 gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt
    34081 ctttctttcc cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt
    34141 gtcaaatgaa tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga
    34201 tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga
    34261 cgatcatcgg tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat
    34321 tttctgtcga tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg
    34381 tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc
    34441 gtttaacggt agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct
    34501 tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt
    34561 tggggttgga aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag
    34621 ctattacgtt attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca
    34681 ctgcagcgct tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca
    34741 ctgagcagaa tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg
    34801 ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa
    34861 aacagcagct cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat
    34921 taaaagacga tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa
    34981 cgcccaccct cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt
    35041 gtttcttcga aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc
    35101 ttgatgccat gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc
    35161 tttccggaga aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca
    35221 tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc
    35281 tgccctctga cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg
    35341 atttgaaagt acaaacgatg atgacttctg aatctacgat gaaagatgcc aaactggcag
    35401 cagctcaagg agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg
    35461 gacgttttgg acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc
    35521 tccatccaga caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct
    35581 gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata
    35641 tctacatgga tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa
    35701 gcggcaggat cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc
    35761 attttccagc tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg
    35821 tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc
    35881 cagaaaaagc gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt
    35941 tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac
    36001 tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat
    36061 ctatccgtac gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc
    36121 ttttttctga attctcttca aaagactctc cttattctat cagctgacag tttctctatc
    36181 ctctagaaaa aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat
    36241 caatgcaaaa aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc
    36301 ctttttcaat cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact
    36361 tattttgccg taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct
    36421 cgcatagatc acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat
    36481 tcctcgtgtg actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc
    36541 cagccttctt aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc
    36601 ttctatttga ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc
    36661 gttatctgct atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga
    36721 agttcctgct aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca
    36781 agtcattgat ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat
    36841 agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag
    36901 catatctaaa taatccattt attttcctcc tttttattta tcctacttac tttttcctaa
    36961 ttctaggata cctacttata tgttactaca cacccttatt ttttacactt taacagtcac
    37021 catcatcaat agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag
    37081 aagcaacaat atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa
    37141 cagtttaaga ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg
    37201 aaaaatgaaa aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc
    37261 gtatggtgtg gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa
    37321 tgatctaact ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa
    37381 aaataagaca actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc
    37441 aaaaaataat tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat
    37501 acaaaaaact gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa
    37561 taaatttgtc gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa
    37621 tggatccagg cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga
    37681 atatattgat ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac
    37741 gatcactcca gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat
    37801 cgatttaact gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt
    37861 aacgatgaat acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt
    37921 catcaacagt tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa
    37981 tcatgaaaca gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac
    38041 tgatttgaca atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg
    38101 cattactcaa aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac
    38161 caatcgttgg gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac
    38221 aagcgacagt acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga
    38281 cagt
//
LOCUS       DARBER010000013        37144 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARBER010000013 DARBER010000000
VERSION     DARBER010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.013"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            80..1081
                     /gene="ccpA"
                     /locus_tag="SCK06_000708"
     CDS             80..1081
                     /gene="ccpA"
                     /locus_tag="SCK06_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG1150733.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1418..3763)
                     /locus_tag="SCK06_000709"
     CDS             complement(1418..3763)
                     /locus_tag="SCK06_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1150734.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            4042..4944
                     /locus_tag="SCK06_000710"
     CDS             4042..4944
                     /locus_tag="SCK06_000710"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG1150735.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            5157..6932
                     /locus_tag="SCK06_000711"
     CDS             5157..6932
                     /locus_tag="SCK06_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG1150736.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6998..8584)
                     /locus_tag="SCK06_000712"
     CDS             complement(6998..8584)
                     /locus_tag="SCK06_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG1150737.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8835..9059
                     /locus_tag="SCK06_000713"
     CDS             8835..9059
                     /locus_tag="SCK06_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG1150738.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9201..10952
                     /locus_tag="SCK06_000714"
     CDS             9201..10952
                     /locus_tag="SCK06_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1150739.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            10952..12736
                     /locus_tag="SCK06_000715"
     CDS             10952..12736
                     /locus_tag="SCK06_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150740.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            13062..13913
                     /locus_tag="SCK06_000716"
     CDS             13062..13913
                     /locus_tag="SCK06_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1150741.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            13967..15718
                     /locus_tag="SCK06_000717"
     CDS             13967..15718
                     /locus_tag="SCK06_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1150742.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            15722..15892
                     /locus_tag="SCK06_000718"
     CDS             15722..15892
                     /locus_tag="SCK06_000718"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1150743.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(15932..16834)
                     /locus_tag="SCK06_000719"
     CDS             complement(15932..16834)
                     /locus_tag="SCK06_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1150744.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            17065..18468
                     /locus_tag="SCK06_000720"
     CDS             17065..18468
                     /locus_tag="SCK06_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1150745.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            18468..19901
                     /locus_tag="SCK06_000721"
     CDS             18468..19901
                     /locus_tag="SCK06_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1150746.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            20046..20972
                     /gene="trxB"
                     /locus_tag="SCK06_000722"
     CDS             20046..20972
                     /gene="trxB"
                     /locus_tag="SCK06_000722"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG1150747.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            21186..22913
                     /locus_tag="SCK06_000723"
     CDS             21186..22913
                     /locus_tag="SCK06_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG1150748.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            22928..23278
                     /locus_tag="SCK06_000724"
     CDS             22928..23278
                     /locus_tag="SCK06_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1150749.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(23370..24230)
                     /locus_tag="SCK06_000725"
     CDS             complement(23370..24230)
                     /locus_tag="SCK06_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG1150750.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            24322..24750
                     /locus_tag="SCK06_000726"
     CDS             24322..24750
                     /locus_tag="SCK06_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1150751.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            24912..25088
                     /locus_tag="SCK06_000727"
     CDS             24912..25088
                     /locus_tag="SCK06_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG1150752.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            25115..25561
                     /locus_tag="SCK06_000728"
     CDS             25115..25561
                     /locus_tag="SCK06_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG1150753.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(25715..27094)
                     /gene="brnQ"
                     /locus_tag="SCK06_000729"
     CDS             complement(25715..27094)
                     /gene="brnQ"
                     /locus_tag="SCK06_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1150754.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            27800..28771
                     /locus_tag="SCK06_000730"
     CDS             27800..28771
                     /locus_tag="SCK06_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG1150755.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            28795..30987
                     /locus_tag="SCK06_000731"
     CDS             28795..30987
                     /locus_tag="SCK06_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG1150756.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            31004..31480
                     /gene="ybeY"
                     /locus_tag="SCK06_000732"
     CDS             31004..31480
                     /gene="ybeY"
                     /locus_tag="SCK06_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG1150757.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            31464..31859
                     /locus_tag="SCK06_000733"
     CDS             31464..31859
                     /locus_tag="SCK06_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG1150758.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            31874..32773
                     /gene="era"
                     /locus_tag="SCK06_000734"
     CDS             31874..32773
                     /gene="era"
                     /locus_tag="SCK06_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG1150759.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            32916..33728
                     /gene="recO"
                     /locus_tag="SCK06_000735"
     CDS             32916..33728
                     /gene="recO"
                     /locus_tag="SCK06_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG1150760.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            34112..35029
                     /gene="glyQ"
                     /locus_tag="SCK06_000736"
     CDS             34112..35029
                     /gene="glyQ"
                     /locus_tag="SCK06_000736"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG1150761.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            35031..37106
                     /locus_tag="SCK06_000737"
     CDS             35031..37106
                     /locus_tag="SCK06_000737"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG1150762.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        12075 a         6634 c         7865 g        10570 t
ORIGIN      
        1 tgagattatt tgatattcta ttttagaaat gtaagacgta ttttcagaaa acatataaaa
       61 tatgaatagg agaacacaaa tggaaaagca aacaatcact atctatgatg tagcgagaga
      121 ggcaaatgtt tcgatggcta ctgtttcacg tgtcgtaaat ggcaatccaa acgttaaacc
      181 agcaacaaga aaaaaagtct tggaagttat cgatcgcttg gattatcgtc caaacgcagt
      241 tgcgcgtggc ttggcaagta agaaaacaac aacagttggt gtaatcatcc ctgatgtcag
      301 caatatgttc tttgcttctt tagcacgtgg tatcgatgat gtggcaacaa tgtacaaata
      361 caatattatc ttagcaaatt cagatggcaa cgaccaaaaa gaagtcaatg tattgaataa
      421 cttactggct aagcaagtgg acggcgtgat cttcatgggt caccatatca cagacgaaat
      481 ccgtggagaa ttttcacgtt caaaaactcc tgttgtatta gctggctcta ttgatccaga
      541 tgaacaagta ggtagcgtaa acattgatta tgtatccgca acaaaagatg cagtaaacaa
      601 attggcaaaa agcggcaata aaaaaatcgc ttttgtcagt ggtgcgttga tcgatcccat
      661 caatggacaa aatcgtctga aaggctataa agaagcatta agcgaaaata acctaccata
      721 tagcgaagga ttgatctttg aagcacaata caacttcaaa gatggtctat ctctagtcga
      781 tcggatccat aatagcggtg caacagctgt ctatgtttca gatgatgaat tagccatcgg
      841 tattttggat ggtttattag accgtggcgt gaaagtaccg gaagaattcg aaatcatcac
      901 aagtaacaat tcactactga cagaagtagc tcgtcctcgt ttaacaagta tcacacaacc
      961 attatacgat ttaggggcgg tatctatgcg cctattgacg aaaatgatga ataaagaaga
     1021 agtcgaagaa aaaacgatta tcttgcctta tggaatcgaa gaaaaaggat ctacaaaata
     1081 ataaaaggac aaccattcag aggttgtgtg cctgccgttt aactctgcgt tacaaggagc
     1141 aatttcaaaa aacagcatct catgtttgat gaatattgtg actcgtgatt cggagttggc
     1201 aggagaacac cgtttatttg ggaaaagggg ttatgacaaa attttgttat aatccttttt
     1261 ttgtgtctta aaaaagagag aaagtggttg aaattacatt tatcgtatat aaaacaatcc
     1321 gtgtatagat gctgcatttt gttcaccacc atcaaaaaaa taagggactg tgacaatact
     1381 attgtcacaa tcccttatta ctgtgtattc tattaaatca atcatctttc tcatctgatt
     1441 ctttagttgc ttgattcgct cttgcataag ctgaagcggt gttccagtaa tctgtatagt
     1501 tttcatcggt accgccaacg ccaaatttaa aggcgctttt cttcggtcca tttttcgccc
     1561 atagcgattc aactgttttt cctggtgttt ggatggattt tttattgaca gtaacttttc
     1621 caggtgtttg accagtaaat ccagatactt ttttcttcat cacatcagat gataattcga
     1681 atttctcatc agtgccaaaa atgtcaggtt ttgcttggta agcttgattg atcagattgg
     1741 caagataatt agccgaacgt atccttgctt tgctgtccat tccagtattg tcatcgtgcc
     1801 ctgcccagct actgatggta atagacggag tagagacaat tagccatgaa tctcggtatt
     1861 cattggtaga accagttttt ccgacccaat ctgcttttcc tagatttccg tttaagcttg
     1921 agatggcatc tttgaatggt gtagtgattt ttgcattgat cacactacgc atcatgtcat
     1981 tcatgatcga tgccgttgct tccgaataga tccggacaga gttgctttcg tgtttataaa
     2041 ttacatttcc tgcgttatct gtgatggaat cgatgatata gccttcttca tagacgccgc
     2101 cgtttgctaa agcctgaaat ccgttggtct gttgaagcgt cgtgacatct actgtaccta
     2161 aaggagcaga ttcaacgccc caattgtcat ttgccgggta gttcatcttt gccaagtagt
     2221 gctcgtaagc atattgtttc gatccaccgg aattcaacac atcttgatat aagtgataag
     2281 ccgggatatt gttggaccat tctagtgact cacggacggt ttggaaggtg ttcgatcctt
     2341 cgttagtagc attgacgatt ttctcgccag cattttctcc ttcttgccaa gttgttgcat
     2401 aatctgatac acgggattct gatccgataa gccccatgtc gattgcaggt ccataaacaa
     2461 gaactggttt gattgatgag ccagcttgac gttctgtatc gaaggcatgg ttattttgat
     2521 tctgactgta attccgtccg ccaacgaatc cgtagatgcg gccagttctg ttatccatca
     2581 atacatttcc ggtttctact tgactgccag caccatcatc taacatgtat ccataatttt
     2641 caacgccatt ttgcaatgca gcatagacat ctttatcgat tgtcgaatgg attgtatatc
     2701 ctttattttg gatcgtttgc tgtgcctttt gataataagc gtcactgacc gatgaatcag
     2761 acatatcagc gttgtctttt tcagccaatt gttgtgcaat gattcttgtt gcttcattca
     2821 tcacagtata atacaagaat tctctgtcag tctgttctgc gatttgttgt ggtagaaagt
     2881 cttttgtaag gtcataggct tttgcttctt cgtattgttc tttcgtaatt tggccttcac
     2941 gatacatact gaataaaaca aaatcttttc tggcaaggcc ggcagaaaga tcttctttta
     3001 gcgcgcctgt gttcgtgtat ggactatacg tgatcgggct ttggggtaat ccagcgatat
     3061 aagcagcttg cggcagcgtc acatcttttg catctacacc aaagattcct tttgcagctt
     3121 cctggactcc agcaatgttt tgtcccttat tattacgacc aaatggtgag acattcaaat
     3181 acgtagcaat gatctcatct tttgaaaagt atttttctac ttgtgcagaa agaatgatct
     3241 cgtttgcttt acgtttgaat gttgtttcat ctgtaaggat ctgctgtttg actaattgct
     3301 gtgtcaaagt tgatccacca gaagagccaa taccagtagc ttcagatact aatgctcgta
     3361 aaacagcttt aggaacgtaa ccttggtgtt tatcgaagta ttcatcctct gtgctgataa
     3421 ttgcagtttt caataaaggt gaaatcttat cagatgaaat agtcgtgcgc atcagatctg
     3481 tctggatttt tgatatctct gtattatctg cataaacaat ctttgacgtt tctgtaatgt
     3541 ttcctagttc tgtctgcagt gcttttttag tcggcaattg tgtgtcttcg acgagatagg
     3601 caaaatagcc agcgcctatt ccaataccta gagctgcgaa catcaaaaga agaacagtaa
     3661 taaaaacagt caatgattga aaaactctaa aaataatctt aggaacaaac catttttctt
     3721 tttttcttct attctgtttt tttcgagtat ttttgcgtgg caaaagggtc acctcaacta
     3781 atggttttat ttcgctaatt atagcaaata atagaacaaa aaaacagaca aggctgtttt
     3841 tggcaggaat ttctttttta aaagagttaa aacgtgagaa aatgatcttt attataaaag
     3901 agttcttaag cttttcttta gagaaaattt cagttactat ggagaacagg gcaaagaaaa
     3961 atcaatcatt ttctttgtat atcatcttac caatatatag gaattgttag taacaattgc
     4021 ctttcaagga ggaatccatc catgttatta ggctcacacg tcagcatgag cggaaagaaa
     4081 atgctgttag gagcagcaga agaagcagcc agttatggat cttcgacttt tatgatctat
     4141 acaggcgccc ctcaaaacac tagaagaaaa gccatcgaag agatgaatat tgatgccgga
     4201 aaagcgtaca tggcagaaaa aggtctaaca aatatcgtcg ttcatgcccc ttatatcatt
     4261 aatttgggga atacgatcaa accagaaatg tttccttttg ccgtccaatt tctgagagaa
     4321 gagattgttc gtgcggaagc tttaggcgct actcaaatta cacttcaccc aggtgctcat
     4381 gtaggggcag gaacagaagc cggaattgct agaattattg aaggcttgaa tgaagttttg
     4441 acaaaagaac agacggcaca aatcgctttg gagacaatgg caggtaaagg tacggaaatc
     4501 ggtcgaacat ttgaggaatt agcgcaaatc atcgatggag taaaattgaa tgaaaaatta
     4561 tccgttactt ttgacacctg tcatacgaat gatgccggtt ataatattaa agaagacttt
     4621 tcaggtgtaa tggaagagtt tgataagatc atcgggttag atcggcttaa agtactccat
     4681 atcaatgatt ctaaaaatcc gcaaggttcg cataaagacc ggcacgccaa tattggtttt
     4741 ggaacaatcg gattcgacac gctgaataag atcgtccatg atccaatgtt tgacaatatt
     4801 tcgaaaatct tggaaactcc ctatgtaggc aaagataaaa agaaagctta cgcaccatat
     4861 ggaaaagaaa tcgccatgtt caaagcgcaa acattcgatc cagaagtgtt tcctgagtta
     4921 ttagcagaag aagtgatact ctaagatttt ctatctgaca gcagcaaaaa aggaccaggc
     4981 acattgcctt gtcctttttt gtttaagaaa caaaggagta ctttttgcag tctttttcat
     5041 catgcatctt ctcccccctc tttttaaatt tagacaaaca aggtatactg gtagaaaagt
     5101 aaaaaaacga aaagtttcac aagcaagtgg caaaacgtga ttggaaggac gaaaagatgc
     5161 tgaacacttt aaaacccttg atcaaccaac taaaaaagaa acatacatac tttattcttg
     5221 gcatagccct attatttaca attattcgtt tgtatttata tagtaaagca atctatggaa
     5281 cagacatgtc ggcaacctat gatgaccagc tgctgattcg ctatgctcaa aatattttgg
     5341 acggtaaatg gctaggggag tatacgacta caacactttc caaaggaatc tcctacagct
     5401 tatttttagt acttgcacat aaactgcatc tttcttatgc agttttgttc ggcatgttca
     5461 gtatttttgc cagtatattg gttgcactcg cgtttaaacc gattgtgaag aacaagtatc
     5521 ttcttttaag tatctatttt tattttttgt tttctccgat tagttttaca catgaatatt
     5581 ccactcgaac atatcgtaat gccctcgtga ttccggctgt tgagatcatt atcgcttgtt
     5641 tattagctat ttattaccgc aaatacggtt ctagcagaaa attgctgcca tggtttttag
     5701 ggctttctgt cgcttttcct ttcttttggt tcattcgcga agattctatc tggcttcttc
     5761 cttttactgt agtagctttg ggtatcagtt tcgtccagat cttgctaggg caagactttt
     5821 ctttcaaaga agtaaagaag ttaaaaaaac gactctttct tctctcgaaa cgggaatggc
     5881 tgaaactttc attgttttta cttcctatag ttggcttttc catcgtctat caatggatcg
     5941 aatataaaaa tgagacaaca tacggtattg caaccatcaa tgaccgttcc agtggagaat
     6001 ttggacgttt gatgaaacaa ctgatccgaa tagatgatga aacgaacttg aataaagaaa
     6061 acagtgagat atgggtctca catgctgcac taaataaagc attagccgca tctcctacat
     6121 tccatactgt ttctacccgt attgacgaat tatatacgac tcacgcgtgg actcaaggtg
     6181 gaaaagtaaa agaattaccc ggagatatca tcttttgggc attgcgagat gtggtaagcg
     6241 aatctggtta ttaccaaaac aatgcaaaag aaaccaatga tttttggaaa aaagtcaacc
     6301 aagaattaga agaagcctat cacaagggaa caatcaaaaa gaaaaaagag ttctatctga
     6361 tgggatcagg agatggaaag acgatgagtg atctgccaat cgtattcgat ttccttcaat
     6421 caggatacgc ttatgcatgg cgatataaag atttccatca aggaggaaat tatagtttcg
     6481 gaacgcaaga acaagtcaaa caggctcaag aattattaaa ccttccgcta atggagaatt
     6541 ggatcgatag taatgaaccg agaacaccta atttccaatt aacaaaatca gcaaaagtcg
     6601 cgaatgtcat cataaaaatt taccaagttc tggctattcc cgtgctgatt gcttcaatag
     6661 cagggatatt acttatgata ctaggaaccg tatttgcaaa agctgatcgt gcctattata
     6721 gttcctcttt gattgtggtc accggattaa tactgacaca attggttttt cttttcggtg
     6781 tctcatggtt ttgttcttat gcgccagaaa aaaaggacta ttttttaagt gtctacactg
     6841 gtgcaggcgt gccattaacg caattcatcg tagtaacaag catgctagtt ttcagtaaat
     6901 taaatctgaa aaaagcttta tcttctaaat aaaaagaccc ttggtagcta gatttttaac
     6961 ctaactatca agggtctttg tgtggaaaaa tcagttttta ttcgatttct tctaccgttt
     7021 cctgcaaaag ctgttcatat tttttgtttc ctttatataa atcaccaatc gtccatgcca
     7081 acaggagaag gacaagaaca atgattacat aagcgatgtg atcagccaat gctagttgag
     7141 cggaagtggc ttgctcgccg aagaaacttt cgatctgtcc tggtaatcct cgtaagttca
     7201 agtaagttag actaatcaca gagaaccagc caagaagttt tacgatgaat gaatttttaa
     7261 atcggctacc catctccgct tggctgttgg tcatcatcag taaaggaatc atcgaaaacg
     7321 gcaacgcaaa agccaagaac acctgggatt cattcattaa gttattcaaa gcggcatgtt
     7381 cttcgatcgt tccttttgta cttgtaaaca aaacacaaat caatacagga atcacagaaa
     7441 gcaatcgtgt gaccagtctt cttagccaaa tcgggatacg catatggatg aatccttcca
     7501 tgatgacttg accggtcaac gtaccagtaa tagtagagtt ctggccagaa gcaagaagtg
     7561 ctacagcaaa taaagtggac aagataccag attttgctac agcaatcaaa ataccattgc
     7621 tcatagctga tggatcagat aaagcttcat acagaccaaa aaaggaagga tcttggacag
     7681 aaccggtttt gaagacagcg acacccatga tcaatagcag tgcattgact agaaaagcca
     7741 ttgacaactg gatattagaa tcccaagtag aaaaacgtac agcttgtgct acatcttctt
     7801 cgttttggtg atcgatcttc cgagtttgtg aaactgctga atgaagatat aagttatgcg
     7861 gcatgactgt tgcaccaata atccctaaag ctccagtcag cggagtttcg ccgccgatga
     7921 ccggtgagtc tgagaatgta tgactgttag ggataaagcc agcaaataaa ctgtgccaat
     7981 ctgggttaga caagccaact tgatagacaa aaacgaataa aatgacgaaa attaagcaga
     8041 caaccagtgc ttcgatctta cgaaacccga cttttgtcag tagtaagaga acgaaaacat
     8101 ctaatacagt aataaagacg gcaatcacca atggaatatc aaaaagtaag tagagggcga
     8161 ttgctgcacc aatgacttct gcaatatctg ttgccataat ggctaattca gtcaagatcc
     8221 ataaaatgac acctaatgtc ttacttgtgc gtgctcggat tgcctgtgcc aaatccattt
     8281 ggctcacgat tcctaatttt gctgccatat actgtaaaag catagcaatc agactagaca
     8341 tcaaaataac tgacatcaat aaatattgga aattttgtcc tccggtgatc gaagtagacc
     8401 agttacccgg gtccatatac ccaactgcta ctaacgcacc aggtccagag taagcaagaa
     8461 gtgttttaaa aaaacttttc ccctttggta catcaactgt tccatttact tcagctaaag
     8521 ataaaccatt tgtatgttca ataagtcgat gcttatgaaa ttgtttattg gattgattat
     8581 tcaagaaaaa accacctttc ctatttatct acgattacat tctacacctc aaaaagaaaa
     8641 aattaaagaa aaagattcgg tacgcctaat tttttttcat ggcgcgtctt gctagatgac
     8701 gaaagaaatt ttgttctcct ctaaaaaaac cactattttt tgcatgaaac taaagaatac
     8761 gcctttcttt tttcaataaa ctttagtaaa ataagtcatt gagagcaatt cggaaataga
     8821 aaggagagat ttatatgtta tcaacaggta ttgtcgtact tattgctatt attgctttgc
     8881 taatcggcgc agtaggtggc tttttcctag cgcgtaaata tatgaaagac tatttagaaa
     8941 aaaatcctcc cgttaatgag gacatgttgc gatcaatgat gatgtcaatg gggcaaaaac
     9001 catctgagaa gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg aaaaaatagg
     9061 ttctgaccca acgacttttt acgggctgat tttttcagtc cgttttttat ttcgtaattt
     9121 gttagactat tcagattatt cagcaaaaaa gacaagcaca gacaatccta ggctcttgat
     9181 aaagacaaag gaggagtaaa atgtcgattt ttagaaaatt aggctggttt tttcgtcagg
     9241 aaaagaaaca ttatttaatt ggggttttct cgctattcgc agtggccttg gtacagttag
     9301 ttcctccaaa agtgattggg atcatcattg atgagatcgc cgataaaaat ttatcaataa
     9361 agatcatttt cgggtggatc ttcctattga ttatcgctgc ttttttacaa tatctttttc
     9421 gttatatctg gcggacgaat atttggggaa gtgcagctcg attggagaaa gaacttcggc
     9481 agcagttatt caaacatttt acagaaatgg acaatctctt ttaccaaaaa tatcggactg
     9541 gggatttgat ggctcacgca acgaatgatt tgaatgcgat ccagaatgta gcaggagctg
     9601 gtatcttgac ctttgcagat tcagtgatta cgggcggaac aacgatcatt gcgatgattt
     9661 tgtttattga ttggcgctta acactgattg cgctgattcc tctgcctttg ctggccgtca
     9721 cttcacgagt gctggggtca aaactccatg atgcttttcg tgattcgcaa gaagcttttt
     9781 ctagtatcaa tgataagaca caagaaagta tcactgggat caaagtcatt aaaacatttg
     9841 gacaagaaaa agaagatcta gcggattttg aagaaaagat tgaagatgca attattaaaa
     9901 acaaacgagt caatttttta gactccttat ttgatccatt cattaccttg attatcggtc
     9961 tgtcttatgt tgcaacaatt attttaggtg gaaagtatgt cttggaaggc acgatctcga
    10021 tcgggcagat ggtctcattt attagttata tcggtatgct tgtttggccg atgtttgcga
    10081 ttggtcgatt gttcaacgta ttggaaagag gaaatgcttc ttatgatcgc gtcaatgaac
    10141 ttttgcatga aaagacgcat atcgtggaac aactgggtgc agtgcagacc ccggcaaaag
    10201 gcgagctgaa tgtagctatt tcctctttta attatccgaa agaagaatca ccagcattag
    10261 aacaaatcaa gtttgcgatc caagaaggag aaacattagg tattgttggg aaaactggtg
    10321 cgggaaaaac gacgatcatc aaattactga tgcgtgaata cgatgattat caagggaaaa
    10381 tccaatttgg cggacgttcg atcaaagaat attcattgaa tgctttatta ggatcaatcg
    10441 gctatgtgcc tcaggatcac ttcttgtttt cgatgaccat tcgagataat atccgttttt
    10501 ctaatccaaa actttcagaa gaagaagtag agcatgcggc ttctttagca tttatcaaca
    10561 atgaaatcaa ggaattacca aaaggttatg ataccttagt tggagaacgt ggagtctctt
    10621 tatctggcgg acagaaacaa aggatttcga ttgctcgagc attgatcaaa aatcctgaat
    10681 tattaatatt agatgatgca ctctctgcag tagatgccaa aacagaagaa gctatcttgc
    10741 ataatttaaa agaaataaga aaagagaaaa caactatcat tgcagctcac cgtttaagta
    10801 gcgtgatgca tgcaaaagaa atcattgttg tcgatcaagg gaaaatcatt gaacgaggaa
    10861 cccatcatga gctgcttgca ctaggcggct ggtatgaaca aatgtgggag aaacagcaat
    10921 tagaagcgaa gatagaagga ggcgaggcct gatggatgaa aattatcaat cggaatggac
    10981 aaaatcgatt ccacttaaag aacagaaaaa gatcatcaaa cgactgatga agttcgccaa
    11041 accttttaga agaacgttca tcgttgctat cttatttgcg tttgctttgt ctgtgatcaa
    11101 tgtcctattg ccaaggatca tccaaacttt tatggatgat catctggcta aacagtcggc
    11161 tacgacacaa gttattttgt tttttgctgg tatgtactta tttggagtca tagtaaaaag
    11221 tatcatttgg tttttccaat ggtatttgta ttccatggcc tcattgaaga cctatcagta
    11281 tattagggta aaattatttg aaaagctgca tacactaggt atgcgttatt ttgatcagac
    11341 accggcagga tcaaccgttt ctcgagtgac gaatgacaca gaaacgctat ttgaattttg
    11401 gtacgtcttt ttgatggtat tgactgggat ctttgcagtc gtttcttcat tccttgccat
    11461 gtttcaaatc aatggagaaa ttgccttgtg gactttggtg tttttgccga ttttaggaat
    11521 cgtcatctgg tattaccaga aattcagttc tcgtatttac cgaaatatgc gggaaaaatt
    11581 aagtcagctg aacacgaaat tgaatgaatc gatttccggc atgcgtatta tccagcagtt
    11641 tcgccaagaa gctcgtttag ctaaagaatt tgaacaagtg aacaatgagt atttagacac
    11701 gcgttatgcg atgatcaaaa cgaattcgat tctgttaagt ccgatcatca attttctata
    11761 tgcattggcg attgcgttgg tgttgacttt gtttgggatc gatgcgttgc attctcccgt
    11821 agaagttgga ctgatctatg catttacgac ctatgtccag ggatttttta atccaatgtc
    11881 tcaaatgatg gactttttga gcatcttcac agatgggatg gttgctggta gtcgtatctt
    11941 gaagattatg gatacggaag aatatgcacc acaacaaaac cctggagcag atcaaatgat
    12001 tacttcaggt aagattgagt ttcgcaatgt aagcttttct tatgatggag agcacaatgt
    12061 attaaacaat atctcttttg ttgctaatcc gggagagacc gttgcattag tgggacacac
    12121 cggaagcgga aaaagctcga tcatcaacgt attgatgcgg ttttacgaat tttatgaagg
    12181 tcagatattg attgatgatc atgacattcg agaatatcca atggaagagt tacgtaagaa
    12241 aatggggctg gtcttgcaag atgctttctt gttttatggg gatatcgcag gaaatattcg
    12301 gatgatgaat ccagctatca cagacgaaca gatacgccat gcggctgagt ttgttcaagc
    12361 aaacaaattc atcgatgaat taccaaaagg atatcactca aaagtgatcg aacatggtgc
    12421 cagctattct agtggacaac gacagctgat cacttttgcg cgaacaatcg tgactgatcc
    12481 taaaattcta gtattggatg aagcaacagc aaatatcgat actgaaacag aaggattgat
    12541 ccaagaagga ttggcaaaaa tgcgtcaagg acggacaacg attgcgattg cccatcggct
    12601 gtcaacgatt cgagatgcag aattgatttt agttttagac aaaggtcata tagtagaacg
    12661 cgggaatcat gaaacacttc tagcgcaaaa tggtttgtat gcagatatgt acaaactgca
    12721 aagcagtggt atataaaaga tgttgtgaca gtagaatttt gacctaagca ctagagtgaa
    12781 aataaaacat agcttctcat atttttcatc tgtgagagtt agtgtcgtag tcaccagatt
    12841 taaaaaaaga ttcgacagct agtcagaagt gcctaatagc cactaagaaa gcgttgacaa
    12901 aagcatttgc tttcgataat ataaagaaaa cgatacaact tggatagtga ttgcctgatg
    12961 caagctaaac ctgatgagtc tcccatggtt tcatggacgg ctttttgggt ttttcttttt
    13021 gttttggata cagccctata catcatggaa aggagcaaaa catgctgatt gagaaagtac
    13081 tgaataacaa cgtcgtaata accagggatg aaagaaatca ggaaatgatc gtgatgggaa
    13141 aaggtttggc attcaaaaag aaagtgggtg acccgatttt tgactcatgc atcgacaaaa
    13201 aatttcagtt gacaaatgac gggctttcac aacagttcca agaattggca caagcgattc
    13261 ctcttgagta cttagaaatt tcttgtgaaa tcattgaata tacaaaagag gctctacaag
    13321 tccagttgaa tgattctgtg tatattgcat tgacggatca tttgtatacg gctatcgaac
    13381 gtcacaaaat gggaatattt gtaccaaatg tattgctgta tgatatcaaa caactatttc
    13441 ctaaagagta tgccatcggg aaaaaaacag tgaagaaaat ctcggataaa taccacatca
    13501 agttatcaga agatgaagcc ggatttatcg cattgcattt agtcaatgca caaggcattc
    13561 aaagccagca agacaatacc tacctgttta ctataacaat acaggatatt ctaaatatcg
    13621 tccgctatta ctataagatt acatttgatg aagattcagt ccattttttt cgattcgtca
    13681 cacacttacg tttttttctg acacgcgtcc tagatggaga aaaatcagaa gtgggagaag
    13741 tttctgagga attgtttcag gttattcaaa aaaaatataa ggatacgttt ggttgcgttg
    13801 agaaaatagc agacttttta gccgagacgt tcaactacca gatgtccacg gacgaaaaaa
    13861 tgtatttaac gatccatact gcgcgtttag tgcatgagaa tcgttgtaaa taaaaaaagg
    13921 aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg ctaaaccttc
    13981 cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga gaaaaatcgt
    14041 agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca caaggcttcg
    14101 tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata tggacggtat
    14161 tgtaacggta atgcgagcgg gagggcagta tcaagttgtc attgggaatc atgtgccagt
    14221 cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg tttcaacaga
    14281 aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc agccattcct
    14341 tgctatccta gctgctggcg ggatgattcg tggactgaca gcattccttg tcttcttagg
    14401 cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg attctgtatt
    14461 ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag taaatgaatt
    14521 cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg aagctttgag
    14581 tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg aagcacctat
    14641 ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc
    14701 cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa agttagtacc
    14761 tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa tgcctttagg
    14821 ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg ctggatttga
    14881 aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct tctggcaaat
    14941 tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc aattttctgt
    15001 gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac aaactgcagc
    15061 acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg caataccagc
    15121 cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta cattaccacg
    15181 taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt atagtgggtt
    15241 aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc ccaactatat
    15301 tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg ccattgcgat
    15361 ggtggtatcc tttacgctga cttttttctt ttggaaggat gaagcaggtg aaacagatga
    15421 tatacagaaa aaatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc
    15481 actaaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat
    15541 ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc
    15601 gacgaaacag gcaattggaa tggtttctga aacagggctg gaattgctga ttcatgtcgg
    15661 attggatact gtgcagttgg aagggaaata ttttgaaagc ttagtgcagc aaggataaag
    15721 agtgaaacaa ggcgatctgt tgctacgctt cgacaaaaag aaaatcgaaa aggaaggata
    15781 cagtttagaa acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa
    15841 ccaaggggag acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag
    15901 gtgaagaatg gactaggctt aactattctt tttattccac ttctttattt tcaaaattga
    15961 aaggctcttt catgatggct gagctgatag ggcgtttatc gaatttttga ccgacagaaa
    16021 ttagatggac taaattctgt tgataatttt ctgcaaaaac acaactatat aacacgtcta
    16081 gaagataaca aattgatgta ttgatcgtaa aattaccaat ttttgaatac agtcgctcgc
    16141 gtgtcgtaag tcgcaaagcg gctgtactca ttgcagatag cgttgaatca ccgatactgg
    16201 tcaaagctaa aattggaaca tgattttgtt tcaatatttc tgcggtacga aggatcacat
    16261 tgttttcacc cgtataagaa atcagaatcc cgcacatatc actcgtgcaa ttgtacgctt
    16321 catagaatga ttcaccaaaa gtggtgacaa tcgtgacatc cattttgatg cgcttcattt
    16381 tcagcatgaa atcttgagaa atcaaagtat tcgagttact ggcgaaaatc acgatttttt
    16441 ttgcttgaag cattagttgt ttggcttttt gcagttggtc atgatgaatg agagaaagtg
    16501 tatcatgaat cgtcatctgt tctaaagaag ccattttatt tgcgatcgtc ataataccgt
    16561 ctgtggtgga aaaaggaaga ttcgcatcta cgtcttgaaa attcccttca agataggctg
    16621 tttcagctaa aaaagcatta cgaaaactga cccagccatc aaaacccatt ttttttgcta
    16681 cacggatgag tgtagagggg tggacataac atcgctcagc aatcgcttgg atggtcattt
    16741 cgccaagtgc tggagaatcg cttaagagaa actggatcac ttcattttct gaaggagaaa
    16801 aggagatttc tttgattttt tcacgtaata acatgtagat accttcttct tattttttta
    16861 cgtaaattat aacttttttg aaaatgattt gtaaacggat ttatactata taacttaaaa
    16921 tagacatttt ttgacaagat atttacaaaa atccactaga aaacagtaaa ttttgtaaat
    16981 gttactggaa agaaacagat ttctattgtt tgttttgttt acaaaaaaga taatgaaagt
    17041 gtaaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac gattggtgga
    17101 ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga ggaattaccg
    17161 atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga gatcgttagt
    17221 cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaagatcta tgcaactctg
    17281 gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg tgtcggtcaa
    17341 ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat tgggcaagaa
    17401 acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtcgt tttggcaatc
    17461 gttgaggaca tgaagcgttt atgtccaaat gcttggctga tcaatttcgc gaacccaagt
    17521 gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat tggattatgc
    17581 aatgtgccag tgatggcgat gatgacagaa ccagcaatgc ttggcaagaa aaaagaagag
    17641 ctgatctaca agtttgccgg tctaaatcat ttccattggc acaaagtagc agataataaa
    17701 ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagagaa tggattaccg
    17761 aaaaatatct ttgatatccc gtttttcaaa gaacaattaa aacaaatgaa catgatccct
    17821 tgtggctacc accgctatta ctatcgtgaa gaagaaatgc tggaacatgc tttagaagaa
    17881 tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga gctatttgaa
    17941 ttatacaaag atccagaatt agaccataaa cctgaacaat tgcaaaaacg aggtggcgct
    18001 tattattcag acgctgcctg tgaaacgatt gcttcaatct atggaaataa agaaacacag
    18061 atcgtggtat ctacaaagaa tgatggagct gttcctgacc tgccggcaga ttgtgtagta
    18121 gaagtcactg cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg ttctcttcct
    18181 gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac gatcgaagca
    18241 gctgtgactg gtgattatgg gacagcttta caggcgttta cgatcaatcc acttatacga
    18301 tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacacca gcagtattta
    18361 ccgcaattca aggagacaat cagccgatta aaagaagaag gaatcgaagt tcaagatgaa
    18421 ctggcaagag aattatcagg taatacacat ataatggagg aagtataatg ggattcagaa
    18481 aagatttttt atggggcggt gctacagcag ccaatcaatg tgagggcggt tatgatgaag
    18541 gtggacgcgg actagcaaac gtagatgttg tgccaatcgg aaaagaccgt tttcctgtta
    18601 tcaccggaaa gaaaaaaatg tttgaatttg atgaggatca tttttatcca gcaaaaattg
    18661 caatcgatat gtatcatcgt tataaagaag atattgcttt gtttgcagaa atggggttca
    18721 agacttatcg aatgtccatc gcctggagtc gtatttttcc aatgggggat gagaaagagc
    18781 cgaatgaagc tggcttgaaa ttttatgagg atgcctttaa agaatgccaa aagcacggaa
    18841 ttgagccact tgtgacgatt acgcattttg attgtccaat gcatttgatc aaagaatatg
    18901 gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgtcga gtcatcttca
    18961 atcgctataa aggacttgtc aaatattggc tgacattcaa tgagatcaat atgatccttc
    19021 atgcaccatt catgggcgca gggctgtatt ttgaaaaagg agaaaatgaa gaacaaatca
    19081 aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag attgctcatg
    19141 aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat tatccataca
    19201 cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat tatttcttca
    19261 ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg gagcgaaatg
    19321 gcattcagat cgaaatggaa ccaggggatg ccgcattgct gaaagaacat actgttgatt
    19381 ttatctcttt ttcttattat gcttcccgag tttcgactac cgacaacgaa ctcttggaac
    19441 agactgcagg aaatatcttt gcttctgtca aaaatccata tctgcaagca agtgaatggg
    19501 ggtggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat gatcgttatc
    19561 aaaaaccatt gtttattgta gaaaacggct taggtgcagt cgatacgcca gatgaaaatg
    19621 gttatgtgga agatgattac cgaatcgatt acctggcagc gcatatccag gcgatgaagg
    19681 atgcagtaga actagacgga gtcgatctat taggctatac gacttgggga tgtattgatt
    19741 tagtatcagc tggtaccggt gagatgaaga aaaggtacgg attcatttat gtcgatcgtg
    19801 ataaccaagg aaatggcaca ttagcccgtt caaagaaaaa atcattcaac tggtataaaa
    19861 aagtaattga ttcaaatgga gaaaatcttt cgaacgattg atcaatgatt tataaaagtc
    19921 tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg caagcagaac
    19981 aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta aaggagaatc
    20041 tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact gctgctttat
    20101 atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggtgcccct ggcggccaaa
    20161 tgaacaatac ggcagaaatc gaaaattatc ctggattcga ttcgatcatg ggccccgatc
    20221 tagcttataa gatgtatgaa agtgtctcaa gatttgggac agaaaatgct tatggaatcg
    20281 ttcaagatat caaggatcac ggcagccata aagaagtcat ctgtgaagat aaaagttacg
    20341 aaggaaaaac agtcattatc gcaacaggat gtatccaccg taaattaggc gtacctggcg
    20401 aggaagaatt tgctggacga ggcgtttctt actgtgcagt ctgtgatggt gcgtttttcc
    20461 gtaacaaacg tctagtagtc atcgggggtg gggactcagc agtagaagaa gccatctatt
    20521 tgacacaatt tgcttctgaa gtagtgatcg tccaccgtag agatgaatta cgtgcacaaa
    20581 agatcatcca agaccgtgca tttgcaaatg aaaaaatttc ctttatgtgg gatacagtcg
    20641 ttgacgaaat cgttggaaac gaaatggtag tgacaggtgt gaaagcacgt aatgtcaaaa
    20701 ccggcaatga atttgattta gcagcagacg gagtattcat ctatgttgga ctggatccat
    20761 taactgcacc ttttagagaa gcaggtttga cgaacgaagc tggctggatt ccaacagaca
    20821 acgaaatgaa aacgaatatg ccaggcgttt ttgccatcgg agatgttcgt gaaaaagatt
    20881 tgcgacaaat cactacagct gttggtgacg gcggtattgc tggacaacaa gtgtataaat
    20941 acttggagtc tttagaagaa acaacagcat aagtttaaaa agagtagaag aacgggttca
    21001 attgttcttt tactcttttt tttcgcgtga aatttactga atctgattta tgcgaacacg
    21061 tccaacacct tttgataaaa gaatcctttc ttgatttaag cgttttttgg tcctcatact
    21121 ttcatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa agaggtgtta
    21181 ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat ttagaagaaa
    21241 acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa gatgcttttt
    21301 atgcaccgtt ggaattcggt actgcaggta tgcggggaat cttaggacct gggatcaatc
    21361 gaatgaacat ctatacggta agacaagcaa cagaaggact agcacgtttc atggatacac
    21421 aaggtgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc catcagtcac
    21481 cagaatttgc aatggaagca gccaaaacac tagcacatca caatatcccg tcttatgtat
    21541 tcgaaagtct gcgtccaaca ccagagcttt cttttgcagt tcgtcatttg aagacgtttg
    21601 cgggaatcat ggtaaccgca tctcacaacc cagcggctta caatggatat aaagtctacg
    21661 gagaagatgg cggacaaatg cctcctgcag acgcagatgc attgacgaaa tatgtccgtg
    21721 aagtcagcaa tccgctaaaa gtcgaagtat tatctgacga agaagctaaa catagcgggt
    21781 tgatcacgat catcggagaa gaagtggacg ctgcttatct agaagaaatc aaagcagtga
    21841 cgattgatcg tgaattggtt gaaacaatgg ggaaagattt gaaacttgtg tacacgccac
    21901 ttcatggaac agggaaaatg ttgggcgaac gtgcgttgaa acaagctgga ttcgaacaat
    21961 tcgtactagt tcccgaacaa gctgtggcag atccagattt cactacggtc aaatcgccaa
    22021 atccagaaga acattcagct tttgaatatg ctatacgact tggagaaaaa gaaggagctg
    22081 acttgctgat tgcaacagat cctgatgcag accgtttagg cgcagcggtt cgtttgccag
    22141 atggttctta tgaagtattg actgggaacc aaatcggagc attgtttgct caatatattt
    22201 tggaagcaca tcaatcaaga ggcactttac cagaaaacgc agcggtattg aaatcaatcg
    22261 tttccagcga attgcctaca gcaattgcgg aacactacgg tgctaccatg ttcaatgtgt
    22321 tgacagggtt taaattcatt gctgaaaaaa tccaacaatt tgaagaagac cattcacata
    22381 cttttatgtt tggattcgaa gaaagttatg gttatttagt taaacctttt gttcgcgata
    22441 aagacgcgat ccaagcattg cttttattag cagaagtagc agcttactat aaaaaacaag
    22501 gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctat ttcgcagaaa
    22561 aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct gctttgatgg
    22621 aaaaattccg taaagaagca ccgacagaat ttgccggagc aaaagttatt caaacagaag
    22681 actttaaaga attgacgaga acagaccgtg aaggaaacgt agaaaaacta acaacaccgc
    22741 catccgacgt attgaagtat acattggaag aaaatggctg gatcgctatc cgcccttcag
    22801 gaactgagcc gaaaatcaag ttttatattg gtgtaaaagg aagctctaca gaagacgcag
    22861 ccgaaaaagt agcctcttat gaagcgatta tcaaaaagat gacagaagaa taaaaaactt
    22921 gagactgatg gaaaaagtgg taaactttag aagtcagtct tttttcaagt tggaggtgaa
    22981 tggattgcca gacacgcaaa aatcaaaagt agagaaagtc agtcaattat tcaaaatgtt
    23041 gagtgatccc actcgtctta aaattctgct atatctgaaa gacggggaac aaaatgtgac
    23101 agcaatcacc caagcagtgg aaatggagca gtctgctgta tcgcatcaac tacgtttatt
    23161 aagagaaaat catgtcgtca agtctcatcg cgagggaaaa gcgattttgt atagtcttga
    23221 tgattctcat gtactggata ttttgaacca gacactcaaa catgtggaac atcaataacg
    23281 ataggtgaaa atagataaac actggagtgt gacaacgaag aaatttcgtt gtcacactcc
    23341 agtgtttttt aagaaaaaga caaaaatgat taatcgcttt tttctgttgt ttcagttcca
    23401 taataactat gatcttctag gtctaaagca ttcaaaataa gcgaagccgt ttcttcaata
    23461 gataaagatg ccacattgat cactacacat cctagttttt cataaagatc attcgcaaaa
    23521 tcgagttcag ctctaatttt ttcgatatcg gaataagcag tatcagggtt taatccgtaa
    23581 gagcgcatcc gttctttacg gatattattt aaaatatctg ggttattcgt tagtcccacg
    23641 atttttttag gatcgatttc ccacagctgt ttgggaatat gtgcttgggg aatcaaaggt
    23701 aaattagcaa cttttaaatt tttatttgct aaaaacaagc tcaaaggcgt tttggaagtt
    23761 cgggatacgc ctagcaaaac gacatctgct tctaaaaaac cacgaggatc tttcccatca
    23821 tcgtatttta cagcaaattc cattgctttg atccgtttga aatagttctc attcaataaa
    23881 tggagggcac caggttgccg aattggcttg ataccagtta aacgttcaat ttctgatatc
    23941 ggtggcgtca tcacatcgaa actaaataat tgatgttctt cacaaaaaga acgtgttatc
    24001 tcgatcagtt cttgattgat taatgtatgc aggacaagtg ctttttcttc taatgcatct
    24061 tgcaatgcct gccttaatgt tttttcatct gtcacgaacg ttctgcggaa taggttgaac
    24121 tcaacggtag gatactgagc catagtagct tgcgctagtt ttgtcgcagt ttctccagca
    24181 gagtcggaga tgacaaaaaa agtaacgatt tctgtagaat cggaagacat ggataaaacc
    24241 tcacttgcat tttttttatg tttattatac catactctag aatgcgtact tcgtaatcat
    24301 tttgatttgg aggaataagt catgaaaaaa cagacattga tcgaacaatc attagaaaca
    24361 ttgaaaaata acggttttaa atatactaaa aaaagagaag cattgttaac ttatttagtg
    24421 aagcgtaacc gctacgtatc tgctaaagaa gtttttgact atatgaatca ggaattcaaa
    24481 ggggtaagtt atgatacgat ttaccggaat ctccatgatt ttgcagagat cgatttactg
    24541 gaagaaaccg atttaaatgg tgaaatgaag tttcgttttc attgctgtca gggggaaatc
    24601 ggccatcacc atcattttat ctgtacagtg tgcggaacaa cgaaagaaat ccatatgtgt
    24661 ccaatgaatt atttcgaaga acagctaggt ggatgcacga tcgaagggca tagattcgag
    24721 attttcggaa gatgtgaaaa ttgtcaataa ggatttaaat aaaataacat ttgataattt
    24781 aaaaaaaaag caagatttcc ttgacggtca aaacagcatt agatataata tttacaggac
    24841 agtcttgttt tttgagatat atttcaataa caattacggt tactttttaa gctcggaggg
    24901 agggaattaa catgtcaaaa acagtcgttc gcaaaaacga atcacttgac gatgctcttc
    24961 gtcgcttcaa acgttccgtg tcaaaagcgg gtactttaca agaatctcgt aaacgtgaat
    25021 tctacgaaaa accaagtgta agacgcaaga aaaaatctga agcagctaga aaacgcaaaa
    25081 aattctagtt ctgtgatgaa ttggagttgg tagtgtgtca ctactaagta gattaaacga
    25141 tgacatgaaa gcagcgatga aagcgaaaga taaggaatct ttacaagtca tccgaatgat
    25201 caagtcatct atacaaaacg agcagattaa agaaggtcac gacttgacag aagaagaaga
    25261 actaacggtg ttgtctcgcg aaatgaaaca gcgacgtgat tctttgcatg agtttgaaga
    25321 agcaggacgt gacgatttag ctgaaaaagt aaaaagtgaa attgtaatag tcgaaaaata
    25381 tatgccggaa caactttccg acgaagaaat tcgacaactt gttcaagaag caatcactca
    25441 aactggtgct tcatcaatga aagaatttgg caaagttatg ggtgcaatta tgcctaaagt
    25501 caaaggaaaa gcagatggaa accaagtgaa tgcgattgtg aaagaactat tacaatctta
    25561 attttatcat ttaaactgcg gacagtgatt gtccgcagtt ttttgtgttc gtaaaaaaag
    25621 aactaaaaaa cagctagaag aaagaataag attctttctt ctagctgttt ttttcctgtt
    25681 taatatctct tatgttaaat tgtgtttgat ttggttattc ttctggtagt tcatttgctt
    25741 cttgtttata ccctgatagg cgataggcgt aataaaagag taagctgcca gcgtaaccaa
    25801 gtaacgttgg gaccatccag ccaagtccta atgcagcaaa aggaacgtga tgatgataaa
    25861 atcctagtaa gcttgctacg acagtttgct gactcactaa tgcaggcgaa tttgccaaca
    25921 tatccaatac ggctggaacg gcagaaaaga acatcgttac ttgatagaca aatggactct
    25981 tttggaagaa ttttgctgtt aaggaaagca agattaacgc aagtgacaat ggatacaaaa
    26041 gcatcaatac tggtaaagac cattggatga tattatccaa tccggcattc gcaacgacaa
    26101 aagagacaaa agtcgttaac cgcagccatg tcatataact gactttaggg aacagtttat
    26161 ggaaatcttg tgcaaaagaa acaacgagtc ccatagcggt agtgaagaca cctaatgtga
    26221 ccattacccc taagaagcta gcaccgaagt tacctaaata aaaattagtg atctgagaaa
    26281 gtgcagttcc gccattttct gataaaggca tcttattcaa actcatcgca ccgagaagga
    26341 ccagaccaaa ataaatcatg acttctaacg caacgctcag gctaccagct tttgctgtta
    26401 attctgtgac ttttttatct ttaaaaccaa gatcacgaac tgcatggaca aaagtgacac
    26461 ttaaagcaag taaagctact gcatcaaccg tgttgtatcc ttctaaaacg ccatttgtca
    26521 aagcgttcgt ctgataagaa tctgcagcag attggtttag attccccatg ggttggaata
    26581 aagaaacgac aaaaatgata gctaataatg caagaaacaa ggagtttaaa tactttccga
    26641 catatttgac caaacgtgtt ggatgtacag taaggaaata agcaagtaaa aagaaaagag
    26701 cagagaataa aaacatacct aatgaatcat atttttcagg caaaaagggt ttagcagcca
    26761 tttcaaaagc agttgctgct gtccgcggtg taccaaagaa tggtccgatc gttaaatgaa
    26821 caaggatcaa gaacaaggca gcataccatt ttccgacagg tttagctaag tcatacagac
    26881 catcactttt tgtcactact acagctaaga tagctaataa tgggaaaaga gcgcctgaga
    26941 tggcaaatcc tacaccagcg ctgatccagt ttgatccagc catttgacct aagtggacag
    27001 gaaagatcag attacctgac ccgaagaaca tcccgaagat aagtgaactg accatcaata
    27061 attgcttcca tgaataagtt tttgtttctt ccataaattt ttcctccaat aattatcatt
    27121 ttgagtaaca aaataaacgt tgcagaaact agaaatgcca ctaatatcag attccatgaa
    27181 aaccaccacc ttaaaatata aaaaaaagcc cttttccact tgggaaaagg gctctagatt
    27241 ctagaacggt accacctttt ttcgcttaaa aaagcttctt cattcacgga cgcttttcga
    27301 taaagttatc cgctagcctg ataatggagg cagccacaat gatctacgtt ttcgacattg
    27361 actcggaaat gatgttcgtc tattcgattt attgtcattt ctcacttaca tgactcactg
    27421 ggaataaatc gctgaatagc ctactgtttt cctcgacgct ttgctgtata tttatttgtt
    27481 gacgttataa tacacctgtt ttttttgaaa tgcaagaata tttcaaatat tttgttttct
    27541 ttttttgaaa caagaaaaat acccagagaa tacttgctaa gttagacgga atacttgtcc
    27601 tctatcttat gcatagctta catgatcagc tttttttatt caaacgagac ctttagagaa
    27661 tggaagcagc tattttttaa cgtttcttgg tacttatggt taagcaactc ttttagaacc
    27721 ttttcttatt tgctatatag tttcttctga gtatggtaaa ataggaaaga atgaatcgaa
    27781 aaaaagggga tgtctatttt tgacggaaga gtctagttct ttagagatta gattgacggc
    27841 tgctgatgat gtcagcatgc ttttaggtgc acacgacaag catataaaac tgattgaaga
    27901 aacaacagaa acaaccatcc atacacgtgg agaaatcatt cagatcattg gcgaacaatc
    27961 ccagatttcc ttggctgcct cagtcattcg gacattgcag gaattgatca aacgaggaat
    28021 ccatgtaagt acaccagatg tcgtgacggc actgaaaatg gggcaaaaag gcacattaga
    28081 ttattttgtt gaaatgtatg aggaagagat cgtcaaagac cgtaatggaa aaccaatacg
    28141 ggtaaaaaac agcggccaaa agaaatatgt cgaatcggta gccaaacatg atatcgtatt
    28201 tggcgtagga ccagccggga caggaaaaac ttttcttgca gtagtattag caatcgctgc
    28261 cttgaaaaaa ggtgaagtgc aaaaaatcat tttgacgcgg cctgctgtgg aagcagggga
    28321 aaatctaggc tttcttcccg gggatctaaa agaaaaagta gatccttatc tgcgtcctgt
    28381 ttatgatgct ttataccaaa tttttggatt agatcatacg aaccgattga tggaacgagg
    28441 cgtgatcgaa attgctcctt tagcttacat gcgggggcgg acattggatg atgcattcgt
    28501 tattttggat gaagcacaga acacgacagt tgctcagatg aaaatgttcc tgacacgtct
    28561 tggttatcaa tcaaaaatga ttgtcaatgg agatacgagt cagattgatc tgcctcgagg
    28621 gacaacaagt ggtcttgttc atgcagaacg aacattaaag caaatcaaga aaatcgattt
    28681 tgttaatttt gaagcaagtg acgttgttcg gcaccctgta gttgccgaaa tcatcaaagc
    28741 ttatgaaaag gcagatctcc atcaagaata agctttgctt aggaggataa gcacatgttg
    28801 aataaaacac tgcagaaact acaagaaaaa cttggaaagg cagcattgcc tttcctgctg
    28861 ttcttgtttt ttatcattat ctgcggcgtt acatttagta gtgtcaaaca aaaggccaat
    28921 gatttcagag aaggccaagt agcagaagaa agtatccgtg cgaacaaaac gatcgagaac
    28981 acggaagaaa ccgaacaaaa aagaaaactg gcagcagaag cagtcactcc agaatacact
    29041 taccaaaaag atcttgcaga tgaccagaac aatcggatca agcaactatt tgaattgatc
    29101 gataaaacga atgatgccat caacaaatct tatgacgaaa agattgataa ggcaaaggaa
    29161 gatgaaaata tcccaaagcc tactacagaa gagcggatcg ccgcgttgaa gaaaaatttt
    29221 gaaaatgtga atgcagaaaa tgttgcgttt tatcaaaaac taccgaacaa tttttattcg
    29281 acgatatttg aaatgactga aactgagatc catactgtga gggatgagag tctgaagctc
    29341 attgacgaac agatgagcaa acaagttcgt gaaagtgaat tggaagcttt taaacaagaa
    29401 gcagaagagc aaatccaata tttgaatgtc acaccagcac aacaacaaat gattcgctac
    29461 ttagtggatc aaggtatcgt agtgaatgac gtattgaatg agaaaaagac agaagaattg
    29521 aaacaatccg cacgagaagc tgtacaacca gtgatgatct tccaaggaga aatcattgtt
    29581 cgtgaaggta atcaaatcga tgcttcagct atgaaaaaat tggaattact aggactgact
    29641 aatcaaacaa cttcgatttt tccgctaatt gccatggttt tagctgtttt gcttcaaatc
    29701 ggcgtactga tttataattc acttcaattc caaaatgcag gagaaagaac aaaatacgtg
    29761 cttttctatg tgacagctat gtcagtcagc atagtgctga tgaaattttt ccaattattc
    29821 caaacggaac aagctactta tatcccgctg ttttatcccg ctgcttttgc accacttgtt
    29881 ttaagctttt tcttgaaccg aagagcaggg atcatggctg gtattttcca agcagtatct
    29941 gcacttttca ttttttatga ttcgattggc acgaactttc taacgattat cttgatgtct
    30001 tatgttttct ctgggctcat ggcaacgatt gtcagacgca aacggatctc agaacaagga
    30061 ttatctgctg ccatgtgggt cattattttt cctgtgatga tggatgtcat tttgatcgtc
    30121 tatcaaggaa tgagctttgg tgatgcaacg acttggttaa tgcttgtctg cgggttcact
    30181 ggtgcactct tctcattctt gctgacgatc ggtctacacc catatattga attgatggtc
    30241 aatgatgaca gtgtaattgt attaaatgaa ctcagcaatc caaatcatcc tttattgaaa
    30301 cagttgctgg aagaagcgcc aggtacttat catcatagta tgatggtagc taatctaagt
    30361 gctaacgctg tagcagaaat cggcggacgt tctttgctaa ctcgagtagc ttgctattat
    30421 catgatatcg gcaagatcaa acatgctagc tttttcgttg agaacttgcc ttcaggagcg
    30481 gaaaacccgc acaatttctt attgccagaa gacagtaaac atattatctt tggacatgtg
    30541 acagatggag ccaaaatatt ggaagattat aatatgccgc aaatggtgat cgatatttgc
    30601 aaacagcatc atggaaccac gctaatgcgt tatttctaca tcaaagcgaa agaacgcaat
    30661 ccggaagtaa cagaagagca attccgttat cctggaccaa aaccgcaaag tcgtgaagca
    30721 gggatcgtca atattgctga cagttgtgag gctgcagtac gggcaatgga tcatccgacg
    30781 agcgaaaaaa tcgaaagttt tgtccataat cttatcgaag agcgtatttc cgatggtcaa
    30841 ttagacgata gtggactgac cttgaaagaa atccgaaaag tggaaaaatc attgatcagc
    30901 ggtttaagta gcaccttcca ttctcggatc aaatatccta aaatgaaaac agaagttgag
    30961 aagataaagg aagaacaaga agaatagaaa gaagcgatgt ataatggata ttacctttat
    31021 ggatgaaaca aaccaagtct cagaagagaa aatgaaagaa atcgatgact tgctgcaatt
    31081 tgcagcatct tatttaaagc tgccagagga aacagaaatg tctgttactt tcatggacaa
    31141 tgcggcaatc caagtaatca atcgtgatta ccgtggaaaa gatgcaccga cagacgtcat
    31201 cagttttgct ctggaagaag aaggcgaaga cgaaatccca gtgatttttg aagatggaga
    31261 gaatccattg cctcgtgtac taggagacat catgatttct attgaacgtg ctgctgaaca
    31321 agcagaagct tacggccatt catatgatcg ggaacttggc tttttagcgg tacatggatt
    31381 cttgcatatc aacgggtatg accatatgac accagaagat gagaaagaaa tgtttggttt
    31441 acagaaagag atcttggatg cctatggact caaaagataa aaacgtagag aaaaacaaac
    31501 atttcctgat gtctgtggaa tttgctgtcc aaggagtcaa aaccgttttt gaggaagaac
    31561 gcaatatgag gaaacatgtt gcgttcggta tccttgcttt atttatgggc gtgattttcc
    31621 agcttgatcg gatagagtgg ttatggctgc ttttagctgt ttttctcgtt tggatagtag
    31681 agatcatcaa tactgttttt gaaaatgtag tggacatgtt tacggatttt cattttcatc
    31741 cgattgggaa aaaaatcaaa gatatggcag ctggagcagt tttagtgacc gcttgttttg
    31801 ctgtcatcat tgggttgatt ttatttgtcc ctaaaatata tcatttgttg tttaattaaa
    31861 aaggagaacg tatatgccag aacataaatc agggtttgtc gcaatcgtcg gccgcccgaa
    31921 tgtaggaaaa tccacattat tgaaccgaat cgtgggtcaa aaaattgcca ttatgagtga
    31981 caaggcacag acaactagaa ataaaatcca aggagtatat actacaccag aagctcagct
    32041 gatttttatc gatacaccag gaatccataa accaaagcat cgtctaggag attttatggt
    32101 agaagcagct tacagtgcgt tgaaagaagt ggatgcagtc ttatttatga tcagtgctga
    32161 tcaaaaacgc ggccggggag acgattttat tattgaacgt ctaaaaaacg tgcattctcc
    32221 agtttatctg attatcaata aaatcgataa gatccatcca gacgagttgc tgggaatcat
    32281 cgaagattat tcgacgcaga tgccttttgc acaagtgatt ccgatctctg ctacagaagg
    32341 aaacaatgtc gaacgcttaa tggatgtatt agtcagtgaa atgccagagg gcccacaata
    32401 ttttccagat gatcaagtga ctgatcatcc tgaatacttt atcgtttctg aattgatccg
    32461 tgagaaagtc ttgtttttaa cacgagacga agtgcctcat tctgtcgcag tcgttgttga
    32521 ttcgatgaag cgaaacgaga atgataaaat ccatatccag gccacgatca tcgtcgaaag
    32581 agatagccaa aaaggaatca ttatcggcaa aggcggaaaa atgctgaagc aagtaggaac
    32641 gaaagcaaga aaggatatcg aaaatctttt aggagacaaa gtatttctag aactttgggt
    32701 caaagtccaa aaagactggc gagacaaaaa agtctatctg caagactttg gctaccgaaa
    32761 agacgaatac tgatattctg aacttcttat aggttaagta tagaaataaa ctggataaaa
    32821 atccgcacgt tgagaaaaac ggcggaagat cgaaacataa ataatctgga tcggcagaat
    32881 ctgcttttcc agatttattt tttaggaggg agaatatgag ccagcttgca gaatcgaaag
    32941 gcttgatttt atctagccgt aattacaaag aaaaagataa attagtcaaa atctttacgg
    33001 aatcttctgg caaaatgatg ttttatatca aaggaatcca tcggcaaaat cagccattag
    33061 ctccggcttt gctccccttt accgaagcag tctatatcgg taattttcga agtgaaggtt
    33121 tgtcgttttt aagcagtgtc aaagacgtcc atgcgtttcg gaacatccaa gaagatatct
    33181 ttttgagcgg ttatggtacc tatttgctaa atctagtaga tgcagcaatc gaagatcatc
    33241 aatatgaccc gcatttattc caatttacgc aacaagctct gcagcggatg gatcaaggag
    33301 atgatgcaga aatcatcacg aatatctttg aagttcaaat actgcaacgc ttcggaatcg
    33361 cgccaatttg gacgcactgt gtcgtatgcg gggaaacaaa aggaaaattt gactattctt
    33421 ccaaatctgg cggtgtgata tgcgagaaac actggccaat ggacgaacac cgttatcatg
    33481 cagatcctcg cgctgtttat ttcgttcgaa tgttttcggc tatttcttat gataagatca
    33541 gtggaatcca cttgaaagaa gaaacgaagc aggcaatccg tgacctcatt gaccagttgt
    33601 atgatgaata tgtcggtatt catctaaaaa gcaaaaagtt cattgatcaa atgaagtctt
    33661 gggcacacgt attaaagacg ccagagaaaa atatgcctga aaaagagata cctgaaaaag
    33721 aagaataaat gaaagataca acaaaaaaat caaaactatt tgacaaatac agggacttcc
    33781 gtctattatt aaccgtagat taaatagatc gttcactgag aggatctatt atataaacaa
    33841 gaaaactgca aaatgaatac gtacattgac caagatcagt aaaaaacacg tggtttagcg
    33901 agttcgggag agtggaagcc ggacccaaac gatttttgaa gaggcacttg cgagtacgat
    33961 aaatgaagct gccgaatctt tattcggaag tagggtggaa ccgcgaaatt attcgtccct
    34021 atgtctgtaa tcagacatag ggactttttg tcgttttctt tcctgtattc ttattgacag
    34081 caacgcttaa aaattgaaag gggcaaaaca gatgagtaaa aaattgaccg tccaagatat
    34141 gattttaaca ttacaaaaat tttggtcatc aaacggctgc atgctgatgc aagcttatga
    34201 cacagaaaaa ggagcaggta cgatgagccc atatacattc ttacgggcaa tcggaccgga
    34261 accatggaat gctgcttatg tagagccatc ccgccgacca gctgacgggc gttatggaga
    34321 aaacccaaac cgtttgtatc agcatcatca atttcaagtc gtaatgaaac cttcacctga
    34381 aaatatccaa gagctttatt tagaaagctt gcgtctttta ggaattgatc cattagaaca
    34441 cgatattcgt tttgttgaag acaactggga gaacccatcg atggggtgtg ccggtgtggg
    34501 ttgggaagta tggcttgatg ggatggagat cacacaattc acgtacttcc agcaagtcgg
    34561 aggactacct tgtaaaccag ttacttctga gatcacttat ggactagaac gtcttgcttc
    34621 atatattcaa gaagttgaaa gtgtctatga tctggaatgg gctgatggtg tgaaatatgg
    34681 cgagatcttc aaacaaccag aatatgaaca ttccaaatat tcttttgaag taagtgacca
    34741 agacttgcta ctaagcaact ttgaccgttt tgaaaaagaa gctaaacgct gtatcgatga
    34801 aaatcttgtt catccagcgt atgattatat tttgaaatgc agccatacat tcaatttatt
    34861 agatgctcgc ggtgcagtat ccgtaacaga acgagcaggt tatctttccc gtatccgcaa
    34921 catggctcgt gctgtagcca aaatctttgt ggcagaacgc gaaaaattag gattcccttt
    34981 attgaataaa aacacagcaa atccagaaaa catgaaggag gctgaataag atggcaaaag
    35041 acttattact tgaaatcggg ctggaagaaa tgcctgctca tgttgtcaca ccaagcatga
    35101 aacaattaga agaaaaaaca gcaaaatttt tagatgaaaa ccaattgact tatgatacga
    35161 tcgaaacgtt ttctacaccg cgccgcttag caatcaaagt aacaaacatt ccagaacgtc
    35221 aagcagacag tgaagaagaa gtcaaaggac cagccaaaaa aattgctttg gacgcagatg
    35281 gaaactggac gaaagcagct gaaggatttg tccgcggaca aggtctgacg acagatgcta
    35341 tcacttttag agaattaaaa ggcgtggaat atgtctatgt aacgaaatac actcacggca
    35401 aagaagcgaa agaagtattg acaggtttag cagatgtcat taaaagcctc actttcccag
    35461 taacgatgca ttgggcagaa tacgattttg aatatatccg ccctattcac tggatcgtgg
    35521 cattgttaga tgacgaagta atcccgtttg agatcttaga tgtcaaaaca ggcagagcct
    35581 ctcgaggaca tcgtttctta ggcgaagatg tgacatttgc gcatgcaaaa gaatatgaag
    35641 aaaaattgaa tgaacaattc gttattgctt caccgcaaaa aagacaagaa atgatcgtta
    35701 cacaagcaaa tgaattagcc gaaaaaaatc actgggttat tgatctagac gaagatttac
    35761 tagaagaagt caacaattta gtcgaatatc caactgtctt ttctggcgac ttcgaagaaa
    35821 aatatctttc tgttcctgaa gaagtactag tcacttctat gaaagaacat caacgttatt
    35881 tcgaggtacg gaacgatcaa ggtatgctgc ttcctcattt tatttctgct cgtaatggtg
    35941 atcgggtaaa attggaaaat gtggttcgag gcaatcaaaa agtattgacg gctcgtttag
    36001 aagatgcgga attcttttat aatgaagaca agaaagcttc aatcgaccaa tacgttgaaa
    36061 aactaaaaac tgttactttc catgaaaaaa tcggtagtat ctatgagaaa atgcagcggg
    36121 tacaagtcat tgcccgtatc attggcgaac aagtaggact atctgaggaa gaattgatgg
    36181 atctgcaacg agctgctgaa atctataaat ttgatctagt aaccaacatg gtaggggaat
    36241 ttcctgaact acaagggatc atgggcgaaa aatatgctct gctgaaaggc gagaaacctg
    36301 ctgtagcaac agcgattcgt gagcattaca tgcctatatc cagtgaagga gagctacctc
    36361 agtctacagt aggttctgtt ttagcgattg cagataaatt agatagcatt ttttctttct
    36421 ttgcagttgg gatgattcca agcgggtcaa acgatcctta tgccttacgt cgccaagcat
    36481 acggtgtcgt tcggatcatc gaatcgaaaa aatgggcctt tcctttgtct gttctgcaag
    36541 aaacgattag tgatgttatc agtaaagata cggatcgttt cggcattggt ttatcagaag
    36601 ggcaacaaca ggtgatcgac tttatcaaag gccgtctgcg ccaattattg acaacgaaga
    36661 atattcgtca tgatgtcatc gaagctgttt tgaatgcaga acaaaaagat ctgacaaaag
    36721 tatttgcagc agcacaattg ttcaagcagc atcttgcaga tgaagacttc aaaccatcga
    36781 tggaagcttt gacacgtgtc gtgaacttag cgaaaaaagc ggagcttgaa aaacaatcag
    36841 aagtagaccc tgaactgttc gaaaatgaag cggagaaaga actgcacaaa gcagtagagg
    36901 caatgaaaaa aagctttgct gaccaaacga tcaacgaaaa ttatgatcag cttgtaagcc
    36961 ttcgcccact gatcgaaaat tattttgatc agacgatggt catggcagaa gaaccagcag
    37021 tacgtaagaa ccgattaacg caattaagtc aaattgctgc catggctttg tcgctagcaa
    37081 gtttagatca aatcatcaca aaataatttc taatatgtaa ataaaccaga gaataggcac
    37141 aaaa
//
LOCUS       DARBER010000014        35482 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARBER010000014 DARBER010000000
VERSION     DARBER010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.014"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..1928
                     /locus_tag="SCK06_000738"
     CDS             162..1928
                     /locus_tag="SCK06_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG1150763.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2030..3289
                     /locus_tag="SCK06_000739"
     CDS             2030..3289
                     /locus_tag="SCK06_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1150764.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3436..4737
                     /locus_tag="SCK06_000740"
     CDS             3436..4737
                     /locus_tag="SCK06_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1150765.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4740..5591
                     /locus_tag="SCK06_000741"
     CDS             4740..5591
                     /locus_tag="SCK06_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1150766.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5604..6419
                     /locus_tag="SCK06_000742"
     CDS             5604..6419
                     /locus_tag="SCK06_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG1150767.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6570..7736
                     /locus_tag="SCK06_000743"
     CDS             6570..7736
                     /locus_tag="SCK06_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1150768.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7966..8613
                     /locus_tag="SCK06_000744"
     CDS             7966..8613
                     /locus_tag="SCK06_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1150769.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8697..9641
                     /locus_tag="SCK06_000745"
     CDS             8697..9641
                     /locus_tag="SCK06_000745"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG1150770.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9776..11227
                     /locus_tag="SCK06_000746"
     CDS             9776..11227
                     /locus_tag="SCK06_000746"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG1150771.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11248..12084
                     /locus_tag="SCK06_000747"
     CDS             11248..12084
                     /locus_tag="SCK06_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1150772.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12084..12743
                     /locus_tag="SCK06_000748"
     CDS             12084..12743
                     /locus_tag="SCK06_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1150773.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12869..13558
                     /locus_tag="SCK06_000749"
     CDS             12869..13558
                     /locus_tag="SCK06_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150774.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13564..14520
                     /locus_tag="SCK06_000750"
     CDS             13564..14520
                     /locus_tag="SCK06_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG1150775.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14564..15076
                     /locus_tag="SCK06_000751"
     CDS             14564..15076
                     /locus_tag="SCK06_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1150776.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15332..15880)
                     /locus_tag="SCK06_000752"
     CDS             complement(15332..15880)
                     /locus_tag="SCK06_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1150777.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15969..17149)
                     /locus_tag="SCK06_000753"
                     /pseudo
     CDS             complement(15969..17149)
                     /locus_tag="SCK06_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(17385..18893)
                     /locus_tag="SCK06_000754"
     CDS             complement(17385..18893)
                     /locus_tag="SCK06_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG1150778.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19053..19244
                     /locus_tag="SCK06_000755"
     CDS             19053..19244
                     /locus_tag="SCK06_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150779.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19447..20364
                     /locus_tag="SCK06_000756"
     CDS             19447..20364
                     /locus_tag="SCK06_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG1150780.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20357..21430
                     /locus_tag="SCK06_000757"
     CDS             20357..21430
                     /locus_tag="SCK06_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150781.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21578..22096
                     /locus_tag="SCK06_000758"
     CDS             21578..22096
                     /locus_tag="SCK06_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150782.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22077..22469
                     /locus_tag="SCK06_000759"
     CDS             22077..22469
                     /locus_tag="SCK06_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG1150783.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22545..23480
                     /locus_tag="SCK06_000760"
     CDS             22545..23480
                     /locus_tag="SCK06_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG1150784.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23613..24392
                     /locus_tag="SCK06_000761"
     CDS             23613..24392
                     /locus_tag="SCK06_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1150785.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24385..24702
                     /locus_tag="SCK06_000762"
     CDS             24385..24702
                     /locus_tag="SCK06_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1150786.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24946..26556
                     /locus_tag="SCK06_000763"
     CDS             24946..26556
                     /locus_tag="SCK06_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1150787.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26713..27561
                     /locus_tag="SCK06_000764"
     CDS             26713..27561
                     /locus_tag="SCK06_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1150788.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27792..28271
                     /locus_tag="SCK06_000765"
     CDS             27792..28271
                     /locus_tag="SCK06_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150789.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28304..30532
                     /locus_tag="SCK06_000766"
     CDS             28304..30532
                     /locus_tag="SCK06_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1150790.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30661..32304
                     /locus_tag="SCK06_000767"
     CDS             30661..32304
                     /locus_tag="SCK06_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1150791.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32377..32922)
                     /locus_tag="SCK06_000768"
     CDS             complement(32377..32922)
                     /locus_tag="SCK06_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG1150792.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(32945..33430)
                     /locus_tag="SCK06_000769"
     CDS             complement(32945..33430)
                     /locus_tag="SCK06_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1150793.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33420..34232)
                     /locus_tag="SCK06_000770"
     CDS             complement(33420..34232)
                     /locus_tag="SCK06_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG1150794.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34371..>35482)
                     /locus_tag="SCK06_000771"
     CDS             complement(34371..>35482)
                     /locus_tag="SCK06_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG1150795.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11602 a         6388 c         7100 g        10392 t
ORIGIN      
        1 attaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg
       61 gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgtt tctctatcac
      121 ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt
      181 atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc
      241 ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct ttgtggagat ccatatacca
      301 taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg
      361 ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag gcgcttacag tatgcgttcc
      421 atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc
      481 gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg
      541 atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa
      601 gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag
      661 atccagaccg agaagacttc ttcgggggag atttgcaagg tgtgagggat catttggatt
      721 acttgacgga ccttggcgtc aatggcatct atttttgtcc catatttaaa gccacatcca
      781 atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac
      841 ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg
      901 tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa
      961 aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga
     1021 ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca
     1081 tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt
     1141 attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc
     1201 atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc
     1261 ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga
     1321 tgaattatgc attcacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt
     1381 cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag
     1441 gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta
     1501 acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta
     1561 tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta
     1621 tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt
     1681 ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa
     1741 atgatcgtga agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt
     1801 actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc
     1861 aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca
     1921 tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc
     1981 attataataa gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag
     2041 aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc
     2101 ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt
     2161 ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga
     2221 aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc
     2281 gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg
     2341 tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga
     2401 tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat
     2461 cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa
     2521 agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa
     2581 tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta
     2641 cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc
     2701 aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg
     2761 aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac
     2821 tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta
     2881 tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg
     2941 aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga
     3001 ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc
     3061 aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat
     3121 tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac
     3181 aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc
     3241 agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata
     3301 accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat
     3361 ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt
     3421 agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg
     3481 ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc
     3541 gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca
     3601 ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg
     3661 attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat
     3721 cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg
     3781 atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc
     3841 gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca
     3901 tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct
     3961 aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt
     4021 tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc
     4081 acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc
     4141 ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac
     4201 gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta
     4261 gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta
     4321 gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg
     4381 atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt
     4441 tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat
     4501 acggggaatt tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc
     4561 gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa acttacgact
     4621 ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt
     4681 atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa
     4741 tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact
     4801 tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg
     4861 ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag
     4921 gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga
     4981 tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt
     5041 atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga
     5101 tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc
     5161 tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt
     5221 ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg
     5281 atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc
     5341 tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt
     5401 tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg
     5461 atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga
     5521 tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa
     5581 caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac
     5641 gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga
     5701 tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga
     5761 tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa
     5821 cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga
     5881 tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc
     5941 atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg
     6001 gtatacgccg ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc
     6061 ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca
     6121 ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat
     6181 tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat
     6241 tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag
     6301 ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg
     6361 tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac
     6421 aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg
     6481 aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta
     6541 atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat
     6601 tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat
     6661 acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga
     6721 aagagattac aagtaacgca tctttggcac taggagaagc ctatatggat catcgaatcg
     6781 agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt
     6841 tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa
     6901 acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag
     6961 atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag
     7021 cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt
     7081 tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg
     7141 tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa
     7201 aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag
     7261 gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc
     7321 ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc
     7381 atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc
     7441 ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc
     7501 aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa
     7561 agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa
     7621 tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga
     7681 ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga
     7741 ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag
     7801 tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat
     7861 tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt
     7921 atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt
     7981 atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa
     8041 ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc
     8101 tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact
     8161 cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca
     8221 gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata
     8281 gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg
     8341 gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa
     8401 gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac
     8461 aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg tactacttat
     8521 tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca
     8581 ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat
     8641 ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga
     8701 gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg
     8761 tccactgcat gactacccag gaattagatg aactggctca ttcagaagca ggagcattta
     8821 tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg
     8881 ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag
     8941 agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca
     9001 ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt
     9061 ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag
     9121 cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc
     9181 cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag atggctgata
     9241 tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt
     9301 atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg
     9361 gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga
     9421 aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac
     9481 atctgctatg tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg
     9541 aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt
     9601 caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg
     9661 attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact
     9721 cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa
     9781 tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt
     9841 tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct
     9901 tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt
     9961 ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac
    10021 ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca
    10081 agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa
    10141 aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac
    10201 accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc
    10261 tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg
    10321 gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg
    10381 agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg
    10441 gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca
    10501 tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat
    10561 tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca
    10621 tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca
    10681 gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc
    10741 tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct
    10801 ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga
    10861 aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa
    10921 aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg
    10981 ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac
    11041 accttataca cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt
    11101 gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt
    11161 gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg
    11221 gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact
    11281 agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc
    11341 tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc
    11401 gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag
    11461 gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc
    11521 acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa
    11581 agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta
    11641 cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct
    11701 ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca
    11761 tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt
    11821 attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta
    11881 aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga
    11941 aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg
    12001 acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg
    12061 atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga
    12121 cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt
    12181 ttcttttagg aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc
    12241 ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc
    12301 tgctgtttct gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca
    12361 tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag
    12421 gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat
    12481 tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag
    12541 ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc
    12601 aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg
    12661 cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac
    12721 gttctatgca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
    12781 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
    12841 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
    12901 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
    12961 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
    13021 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
    13081 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
    13141 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
    13201 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
    13261 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
    13321 gcaaatggag cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag
    13381 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
    13441 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
    13501 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
    13561 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
    13621 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
    13681 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatcgaat cgctgtgatc
    13741 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
    13801 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
    13861 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
    13921 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
    13981 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
    14041 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
    14101 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
    14161 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
    14221 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
    14281 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
    14341 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
    14401 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
    14461 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
    14521 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
    14581 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
    14641 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
    14701 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
    14761 tgcattagtc tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
    14821 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
    14881 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
    14941 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
    15001 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
    15061 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
    15121 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
    15181 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
    15241 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct
    15301 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
    15361 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
    15421 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
    15481 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
    15541 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
    15601 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
    15661 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
    15721 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
    15781 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
    15841 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
    15901 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
    15961 tttgtatatt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
    16021 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
    16081 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
    16141 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
    16201 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
    16261 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
    16321 aaaggatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac
    16381 cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca
    16441 acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt
    16501 ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa
    16561 gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag
    16621 aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta
    16681 attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat
    16741 tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca
    16801 taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa
    16861 ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa
    16921 caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag
    16981 aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa
    17041 cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag
    17101 caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc
    17161 tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa
    17221 ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa
    17281 aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga
    17341 ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga
    17401 ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt
    17461 tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt
    17521 ttatcataag caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag
    17581 acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt
    17641 attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt
    17701 tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta
    17761 atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt
    17821 tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga
    17881 cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat
    17941 ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct
    18001 tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta
    18061 ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt gattacagga
    18121 aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag
    18181 gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc
    18241 ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta accagctgca
    18301 tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct
    18361 gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca
    18421 aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt
    18481 ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt
    18541 ccactatagc gagtgataac gacactgttg attaataagc cataaccacg taattcatcg
    18601 attaaccgca agatatccat atcataagta atcccataat caccacggat cttattacgt
    18661 tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc
    18721 aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca
    18781 actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata
    18841 tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc
    18901 cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga
    18961 aattcctcaa aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
    19021 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
    19081 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
    19141 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
    19201 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
    19261 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
    19321 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
    19381 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
    19441 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
    19501 aatgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
    19561 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
    19621 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
    19681 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
    19741 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
    19801 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
    19861 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
    19921 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
    19981 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
    20041 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
    20101 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
    20161 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
    20221 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
    20281 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
    20341 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
    20401 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
    20461 tgacacagaa gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac
    20521 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
    20581 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
    20641 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
    20701 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
    20761 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
    20821 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
    20881 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
    20941 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
    21001 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
    21061 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
    21121 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
    21181 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
    21241 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
    21301 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
    21361 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
    21421 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
    21481 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
    21541 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
    21601 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
    21661 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
    21721 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
    21781 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
    21841 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
    21901 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
    21961 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
    22021 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
    22081 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
    22141 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
    22201 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa
    22261 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
    22321 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
    22381 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
    22441 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
    22501 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
    22561 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
    22621 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
    22681 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    22741 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    22801 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    22861 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    22921 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    22981 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    23041 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    23101 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    23161 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    23221 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    23281 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    23341 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    23401 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    23461 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    23521 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    23581 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    23641 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    23701 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    23761 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    23821 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    23881 gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac
    23941 tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata
    24001 aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga
    24061 tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga
    24121 cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag
    24181 gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat
    24241 tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa
    24301 aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact
    24361 tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt
    24421 ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa
    24481 gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta
    24541 gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata
    24601 gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt
    24661 ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa
    24721 aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt
    24781 caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca
    24841 agctcttttt tattttagga attagagagg gatttgtcct tctatgattt tttgctataa
    24901 tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg
    24961 atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc
    25021 tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca
    25081 ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt
    25141 ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt
    25201 cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca
    25261 ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat
    25321 gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca
    25381 atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc
    25441 gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact
    25501 gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta
    25561 gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat
    25621 tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt
    25681 cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac
    25741 caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg
    25801 gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc
    25861 atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat
    25921 cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt
    25981 ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat
    26041 gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta
    26101 ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta
    26161 actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc
    26221 ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat
    26281 cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta
    26341 aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg
    26401 aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga
    26461 agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg
    26521 aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc
    26581 acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt
    26641 ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag
    26701 gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt
    26761 atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt
    26821 tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa
    26881 gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg
    26941 aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt
    27001 gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac
    27061 tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat
    27121 gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac
    27181 gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt
    27241 gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa
    27301 attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt
    27361 attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat
    27421 aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa
    27481 atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc
    27541 attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca
    27601 tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta
    27661 tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca
    27721 tatagtttag taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag
    27781 gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag
    27841 actatgataa agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg
    27901 gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata
    27961 ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag
    28021 cgatcttagc tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt
    28081 atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag
    28141 aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac
    28201 aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc
    28261 taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact
    28321 aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag
    28381 ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga
    28441 tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca
    28501 attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc
    28561 agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga
    28621 acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac
    28681 actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct
    28741 agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga
    28801 aatcgttcga gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg
    28861 agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt
    28921 accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa
    28981 ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa
    29041 aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca
    29101 aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa
    29161 agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta
    29221 taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac
    29281 tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga
    29341 acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg
    29401 aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat
    29461 tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa
    29521 acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag
    29581 cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc
    29641 tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg
    29701 acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt
    29761 ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc
    29821 acttagccgg caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg
    29881 aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt
    29941 tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga
    30001 atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat
    30061 tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg
    30121 ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg
    30181 gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact
    30241 tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat
    30301 tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat
    30361 ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctattgg
    30421 ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga
    30481 cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat gataaaataa
    30541 agctcaacga acgcacttag cagattcgtt gagcttttcg tttctagtta ccggtaacaa
    30601 ggatacttct aaaatagaat cggttataat caagagggac cgttaaagga gtgaaacgat
    30661 atgatacaag aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta tcctgctagt
    30721 ttcaaagata gcaatggcga tggtcaagga gacatcaatg ggatccgtga aaaacttaat
    30781 tatttgaaag atcttggtat cggctttatc tggatcaatc cgatctatca atctccattt
    30841 gtcgataatg gttatgacgt aagtgactat caagatatag aagagagatt cggaacgatg
    30901 gaagattttg atttattgct aaaagaagca catgaattag ggatcaaagt gattatggat
    30961 cttgtcatca accatagttc ggatcagcat caatggtttg aagaatcgaa aaaatctaaa
    31021 gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg tagatggcaa agaaccgaat
    31081 aattggacat cgattttcgg cggttctgct tgggagtatt ctcatgaaac tggacagtat
    31141 tatctgcatg tttttgcaaa agaacaacca gacttaaatt gggaaagcga gaaattgaaa
    31201 gaagaactat tcaatatgat ccgatggtgg ttggataaag ggatagatgg atttcgttta
    31261 gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga gaaccctttc
    31321 tcaccctttc aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa acatgttttt
    31381 gaagagtacg atatcatgac tgtcggagaa gcaagtggtg tcactgcaga agaaggacca
    31441 caatgggtag ggaaagacgg ttatttcgat atgatattcg aatttgatca tatccatatc
    31501 tggcagcaag aaaaagaagg gcaattagat gtccttaaat tgaaacttgc attaagcgct
    31561 tggcaaactt cattggatgg tatcggatgg aacgctttat atatggaaaa ccatgatgtt
    31621 cctcgtgccg tttcagtgtt tggagatact cgtcccgatt tttgggctat gtctgcaaaa
    31681 gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat ttatttatca aggacaggaa
    31741 atcggaatga cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt agattcgaaa
    31801 agactttata aaaggctgct tgcggaaggt aagacaagag aagaagcatt ggatatcatt
    31861 cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat ggacttcgga acaatatgct
    31921 ggtttttcta cacatgaacc ttggctgatc actaatccaa ataccaaaac gatcaatgta
    31981 gaacaacaag aatatgaacc agagtcggtc ctgcaattct ataagaacat gatccgcatt
    32041 agacaaacca ataaaggtct gatatacggt tcttataaag aatatcttca tgaacatcca
    32101 caactttatg tctatgaaag atatttagaa gatgaacaat atcttattat ggtaaactta
    32161 acagaatcac ttgctgacta tgaacttcca aaagaagcag atcagtcctg gacattactc
    32221 ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca tactagcacc ttatgaagct
    32281 cggttatata aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt ggactttgcg
    32341 aggaagtttt tcagattgct ctgtcaatta ctcatttcaa taattcgctc taggaccacc
    32401 gatatattta ggacgagagc gaagggctgt cacgtgagct tgtccaaggg ttttcaaacg
    32461 aggtctgaca ggtacttgga catgtttgat atgcataccg attgatgtgt caccgatatc
    32521 gataccggct tgtccaacta cgtgttccac ttcgacagga tcattgaact ggtcaaaagc
    32581 agcaacagaa caagcacctc cagcatgtag tgcaggaaca acagagacaa tttcgaaatt
    32641 ttttgcttct gccactgttc gttctactac taatgctcgg ttcaaatgtt cacaaccttg
    32701 gacagctagc gcaatttctt taggatccaa caactctttt aacgtacgga tgatccattg
    32761 accaacttcc gcactggaat tttttccaat atgtcctcca acgacttcgc ttgtgctgca
    32821 tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca gccaatatgt cattaactat
    32881 ttcagtcaat tggtctttta attctttttc tgacaacttc attttctaaa accttctttt
    32941 tcaattaata ttgcttccgt gcttgaatac gttgaagact tgcagttata ggaaaggcaa
    33001 gtcccattcc aatcagattt tggaagatgt ttcctggtat agaagccact ccagccggcc
    33061 aatcatataa aatccaaccg gcgataccat agccgatgat cataactaag cttcctaata
    33121 aaagccctaa tccccacata cgagtagaag atttgctgaa agaaccagca acatatcctt
    33181 gtaacccgtg gatcactaat gaaaaaactg cccactgcgg atatcctgaa atcaaatcga
    33241 tcaatagacc actggaagca ccaacaacta atccacccgt tggaccataa agaaaagcag
    33301 cggtatagat cccggcctcg cataatgtca caaatccatt cgtagccggt acagggatca
    33361 aaattgttaa tgaaagggca actgtcaacg caatcagcat agcatagatc gttaattgct
    33421 tagttttcat ttctcattcc tcctttttga ataccgccag accagacact tccggtatga
    33481 tcgttcatca gcacgccatt agaaatacaa tgggcaacaa attttttact ttctgctact
    33541 gctttggtaa aatctgcccc taacacaaga ttcgctgtta ttgcagaaga aaaggcacaa
    33601 ccggccccgt ttactgttgt agtgtcgatt tttttatgag aaaatacctc ataatgatca
    33661 attgaataga gaagatccac agcttcatct ccagccatcc ttgtgccgcc tttgatcaca
    33721 atgttttttg ttcctgaagc agtgagttct ttagcgagat ggatcatttc tgttaacgaa
    33781 gagggaagag cttgaccaga aagtaaagca gcttctgcca aattaggcgt aatcatcgta
    33841 acaagtggaa aaagttcttc gatgagctgc gaaatataca attgattgat cacagcttca
    33901 gtttctttga atgccaaaac agggtctaaa atgattggga tattttgttt tgtttttatg
    33961 aagtctcgaa caaccatgat tgcttcactt gaattcaata acccaatttt gataccatct
    34021 aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa agatatagat
    34081 tccagcataa atcgctcatt aatatctagt gcccctatag aagtcaaggc gcttagtccg
    34141 aatagaccaa aattctcaaa agtttttaaa tctgtttgga ttcctccacc agcaaatgga
    34201 tcagaaccgc caattgtcaa gattcttttc ataacatttt cctccatgaa taagagtata
    34261 acgaaatttt caaaaaagaa aaacagccaa ttggcatatt tttatctcat ccaatttaaa
    34321 aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt ccttaaatag ttaggacttt
    34381 tgtgatttta attctttctc gatttgaagc cgttgcactt tcattgtagc tgttcgcggg
    34441 attgcatcaa aatcacggat gatagggacg ttcaagcgag gaagttcatg gacttgctcc
    34501 caccatcgat tccaatccat ttctttatca ggagctaaag caataatcgg ttgtggagaa
    34561 tgatttttat cccttacaat cactacttct gccaaaaaat ctaacgaatc taataaaaag
    34621 tcttcaatag ctaatgtact attgatcgtt tcgatcaaat ccacttgccg atctttcaaa
    34681 aataggtggc catgcttatc cataaaaccg taatctccgc tatcccacca atcgccataa
    34741 acattttcct ggaaccgtgc atcttccttg taataagtca aagcccgtcc tttagacagt
    34801 aattgtatat gaccatctgt gttttcaggt aggacattcc caactgaatc tgtaatccga
    34861 gcgcttgtca tgtcttccag tccaacgccc atatctcttg catcacttgt tttcaaactc
    34921 tctagtgtat gagccttaag aatcattggt ccgcattcac tttggccata tacttgaaga
    34981 aataccgcat cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg gttgtttata
    35041 gcatcaaaag tggagtgata gtatcggata cccgaaaaag cttctggatg ctccttagca
    35101 gtaaaacgcc actgaacaaa gttattcgga tgggtctcta gtgcaattgg ccggtgagct
    35161 tccaacatat gaacaacttt tgaactttgt gcgtttgcta aaggcatcat aggaaatccc
    35221 atggacatca aagaagagat accaatattg aatctggaat gcactggaga gatgtgaaaa
    35281 gcgattaatt tcttttccga aatttttgtg aaaactgttt tttgccattt cgtccgccat
    35341 cccatagaat gagcagaatg acaaataagt ttagggatac cagttgtacc agaagtatgg
    35401 gtcatataag aaatttcatt tttgtctaaa aatgattgag aaacagacaa agcaggttgt
    35461 tccattaaat aaagaatagg aa
//
LOCUS       DARBER010000015        34882 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DARBER010000015 DARBER010000000
VERSION     DARBER010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.015"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..188)
                     /locus_tag="SCK06_000772"
     CDS             complement(60..188)
                     /locus_tag="SCK06_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1150796.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(191..1564)
                     /gene="rlmD"
                     /locus_tag="SCK06_000773"
     CDS             complement(191..1564)
                     /gene="rlmD"
                     /locus_tag="SCK06_000773"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1150797.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1675..2730)
                     /locus_tag="SCK06_000774"
     CDS             complement(1675..2730)
                     /locus_tag="SCK06_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1150798.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2740..4170)
                     /gene="gatB"
                     /locus_tag="SCK06_000775"
     CDS             complement(2740..4170)
                     /gene="gatB"
                     /locus_tag="SCK06_000775"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG1150799.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4167..5639)
                     /gene="gatA"
                     /locus_tag="SCK06_000776"
     CDS             complement(4167..5639)
                     /gene="gatA"
                     /locus_tag="SCK06_000776"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG1150800.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5639..5944)
                     /gene="gatC"
                     /locus_tag="SCK06_000777"
     CDS             complement(5639..5944)
                     /gene="gatC"
                     /locus_tag="SCK06_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG1150801.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6050..8086)
                     /gene="ligA"
                     /locus_tag="SCK06_000778"
     CDS             complement(6050..8086)
                     /gene="ligA"
                     /locus_tag="SCK06_000778"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG1150802.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8103..10337)
                     /gene="pcrA"
                     /locus_tag="SCK06_000779"
     CDS             complement(8103..10337)
                     /gene="pcrA"
                     /locus_tag="SCK06_000779"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG1150803.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10492..12051
                     /locus_tag="SCK06_000780"
     CDS             10492..12051
                     /locus_tag="SCK06_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG1150804.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12162..14093)
                     /locus_tag="SCK06_000781"
     CDS             complement(12162..14093)
                     /locus_tag="SCK06_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1150805.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14111..15025)
                     /gene="pfkB"
                     /locus_tag="SCK06_000782"
     CDS             complement(14111..15025)
                     /gene="pfkB"
                     /locus_tag="SCK06_000782"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1150806.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15022..15774)
                     /locus_tag="SCK06_000783"
     CDS             complement(15022..15774)
                     /locus_tag="SCK06_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1150807.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15933..16142)
                     /locus_tag="SCK06_000784"
     CDS             complement(15933..16142)
                     /locus_tag="SCK06_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150808.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16153..17481)
                     /locus_tag="SCK06_000785"
     CDS             complement(16153..17481)
                     /locus_tag="SCK06_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150809.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17617..17772
                     /locus_tag="SCK06_000786"
     CDS             17617..17772
                     /locus_tag="SCK06_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150810.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17846..18433)
                     /locus_tag="SCK06_000787"
     CDS             complement(17846..18433)
                     /locus_tag="SCK06_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG1150811.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="SCK06_000788"
     CDS             complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="SCK06_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG1150812.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20013..21302)
                     /locus_tag="SCK06_000789"
     CDS             complement(20013..21302)
                     /locus_tag="SCK06_000789"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG1150813.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21456..22388)
                     /locus_tag="SCK06_000790"
     CDS             complement(21456..22388)
                     /locus_tag="SCK06_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150814.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22545..23204)
                     /locus_tag="SCK06_000791"
     CDS             complement(22545..23204)
                     /locus_tag="SCK06_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1150815.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="SCK06_000792"
     CDS             complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="SCK06_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1150816.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24245..24535)
                     /locus_tag="SCK06_000793"
     CDS             complement(24245..24535)
                     /locus_tag="SCK06_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150817.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="SCK06_000794"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="SCK06_000794"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG1150818.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="SCK06_000795"
     CDS             complement(26821..28176)
                     /locus_tag="SCK06_000795"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG1150819.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="SCK06_000796"
     CDS             complement(28200..29369)
                     /locus_tag="SCK06_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150820.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="SCK06_000797"
     CDS             complement(29366..30253)
                     /locus_tag="SCK06_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG1150821.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34134)
                     /gene="nifJ"
                     /locus_tag="SCK06_000798"
     CDS             complement(30442..34134)
                     /gene="nifJ"
                     /locus_tag="SCK06_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG1150822.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34333..34854
                     /locus_tag="SCK06_000799"
     CDS             34333..34854
                     /locus_tag="SCK06_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1150823.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9407 a         7571 c         6287 g        11617 t
ORIGIN      
        1 ctaaaaagac taagaaacaa taacaaaagt taacacttct tagtctttct ttagcacgtc
       61 tatttattaa agattttagg gccttttctc atgtttttac gatcattctt aggtgtttcc
      121 atttgtttgg atcggcctcc accttgtttc tttttgatca attctttcat tttttctttt
      181 tggtccattt ttattctcct ttaacgaatg aagcgactaa ttcgatatga tgggtttgcg
      241 ggaaaagatc tacaggctga actttcttca cggcataacc tgcttctgca aataacttag
      301 catctcttgc aaaagttgcc ggattacatg agatatagac gattcgttct ggtgacattt
      361 cgattgctga ttcaataaaa tgatgatcta atccttttcg tggagggtca actacgatca
      421 cagatggttt gatccctttt tcttgccatt ttttcataac tttttctgct tttccgactt
      481 cataatgaac attttcgatt ttattattta aagcattgaa ctgtgcatct tcaattgctt
      541 ctggaataac ttccatccca tacaccttat ccactctgtc tgctaaagac aagccaatcg
      601 tacctattcc tgaataagcg tcgatcacaa tgtctttttt atctaactcg gcaaactcga
      661 tggctgtttg gtacagatac tctgcttgtt gagtatttac ttgataaaat gactttgctg
      721 agattcgata ggtttttcct aatagctgat cttcgatata gtctctgcca tataaaactt
      781 tttctttgtc tcctaaaatc acatttgttt tttcttcatt gatattttgg atgaccgaaa
      841 ccacttctgg caattcttca tgaatagctt cagcaatttc cttggattta aagaattttt
      901 ctttacgagt aactaaaacg accatcattt catgggaata atgaccacga cggataatga
      961 tgtgacgaag ctgtccttca tttttttctt cattatatcc tcgtacttgg aaccgttgca
     1021 agatatctct gactactttg attgcttgat cgatgacagg atcttggata taaaaatctt
     1081 cgatagggac tagatcatgg ctattctttt tgtaaaaacc ggtggctaac tgtccatcga
     1141 tacggcgaac agggatctgt gctttattac ggtagccgaa tggttctgcc attccgaccg
     1201 ttggaagtac aggaacttct ggcatcttag caatcttcgc cattacatgc tctacttgtt
     1261 tttgtttgaa gacaagctgt tgttcatact tcaaatgggc taaaggtgcg atacctatgc
     1321 gaagtaatgc attgttttct acaggctgac gatcaggtga ttgtgtaata aatgtttcga
     1381 cttttccata tccaaactta ttgcctgctt ttactactag tatttctact tcttctccag
     1441 ggagtgcatt ttcgataaat aatggataac catcgatttt tgctaccccc attccttcat
     1501 aactcaagtc gacgatttcg actgtatatc gttcgttttt ctttactatt tgctgttctg
     1561 tcatattttg ctcctttaag cctatttttc aatcctactg aactctacta taaatcagct
     1621 aattacacaa ctaaaaccgg cactgacctt actacgttca gcaccggctt ttgattaatt
     1681 cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg tcgatatctt catccgttaa
     1741 acgttcgact tcttttacga attctttgct gacaacctct aattcttctt catctgtacc
     1801 tgtgattgct tcatcaggaa tctgatcggt attggcaaag aactcaatgt gttgatgaag
     1861 attttggaaa tgcatcggtg cttccccgcc gtattcacca tctaagttga tcattactgg
     1921 tcgattgata tcatcttctg tttcaacgaa caaactgctt gttttggtat agattattct
     1981 tggatcatcg acatgtcgac cgccattcag catgagtgct gcaatatgca agatctcaaa
     2041 gatatttgct gtcttcacaa tgattaaaga aaacttccca tcatccaatt tggcatctgg
     2101 cgcgatctgt tcgaatcctc caacagagtt cgtcagccct aggaaaaaca tcgaagcatt
     2161 tccgatatat tccccttcat catacttcaa gcgcattttg attggcttta ctcgagggag
     2221 catctctgct cctttcgcaa gataagcaag ataaccaaaa atactcttta gttctgatgg
     2281 aacttcataa gtcaattctg tcaaatagcc acctgcagcg atattgatga agtaactgtt
     2341 atcagaaagg ccgatgtcca tttttacggt ttggtgtttt tttatcactt ctgctgctgc
     2401 tttaatatta tctctaggaa tttttaatgc tcgagcatag tcgttcgttg taccagctgg
     2461 gatgatggcc attttaggac ggtgttcaag cggagcgatt ccattaacca cttcgttgat
     2521 tgtgccatcg ccaccagctg ccactactag atcaaaacct agttctgcga ctctacatgc
     2581 ttcattcttg gctgaatctg gttctggtgt agttgcatag gcgctggctt caaatcctgc
     2641 ttcttctaat gcctgtagga tatccgccaa attttttttc aataattctt ttcccgaggt
     2701 ggggttataa attacgcgtg cttttttcat tttcagcctc tatcgtttcg ctaattcatc
     2761 tttaagtagc ttattcacga caccaggatt tgcttgtcct ttagttgctt tcatgatttg
     2821 accgactagg aagccaaccg cacgatcttt tccatttttg aaatcatcga cagattgttg
     2881 gttgttatcc aaaatttcat taatgattgg cagtagtttg ttcggatcag ataattgcac
     2941 ccaaccattt ttttcaacta cttcttgggc ttctccgcca tttgtgatca attcacggaa
     3001 gacttttttg gcgatttttg agctgatcgt tccatcggcg atcaaacgaa tcattcctgc
     3061 taagttctca ggtgtcaatt ttgtctcagc taattccagt ttctcactgt tcaagtaagc
     3121 agaaacttca cccatcaacc agttagatgc ttgcttggca tctgcaccat tttccaaaac
     3181 agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat accattgcgt catactctgg
     3241 aagtccaaac tcacggatat agcgttcgcg acgagaagct ggcatctcag gcaaactagc
     3301 ttttactttt tgaatccatt cgtcatcgat cacgaatttc gggatatcag gctctgggaa
     3361 gtaacgataa tcactagacc cttctttgac acgcatcaaa actgttttat tcgttgtttc
     3421 gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa agaagaactt ttgcttgacg
     3481 tttttcttca aaagctaaac cttttttcac gaaagataaa gagttcaagt ttttcaattc
     3541 tgctttcgta ccgaactctt cttgtccata agggcgaaga gaaatattgg catcacagcg
     3601 catagagcct tcttccattt tcacatcgct gacttctgta aattggataa ttgatcgcaa
     3661 tgcttctaga taagcgtatg cttcttctgg tgaacgcata tcagcttcag aaacgatttc
     3721 gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa taaccgccaa taccatgcat
     3781 gttttttcca gcatcttctt ctaaatggac acgttcgatc cggatttttt tctttttacc
     3841 ttctacttcg atttcgatcc atccgtcatg accgattggt tgatcaaatt gggaaatttg
     3901 atatgctttt ggattatctg ggtaaaagta atttttccgg tcaaaatgag tatctttaga
     3961 gatttcacag tttaatgcca aagctgcttt catcccgaat tccagcgcag ctttattcat
     4021 tactggcagc acgcctggat aaccccagtc gatcacgttt gtattgctgt ttggttctgc
     4081 accaaaatga gctggcgctg gagagaaaat ttttgagttg gtttttaact cgacgtggac
     4141 ttctagtcca atgactgttt caaaattcat tcgtctttcc ctcctaaaat agctggttgt
     4201 tgtttatgga attctgttgc ttgttcaaac gcatatgccg cttgatacat cgttttttca
     4261 tcaaaataat taccgatgat ctgcaatcca actggtaatc cttctgataa acctgccggt
     4321 acagacatac ctggaagtcc agccaagttg acagggatcg ttaagatgtc actcatgtac
     4381 attgtgattg gatcgttaat gttctcaccg attccgaatg caactgttgg tgaagatggc
     4441 ccgatgatca agtcataatc tgcaaagact ttttcaaaat ctcgtttgat caatgtacgg
     4501 acttgtcctg cttttttgaa gtaagcgtca taataacccg cgcttaagga gaatgtacct
     4561 aacatgatac ggcgttttac ttcttcaccg aatccttctg aacgtgagtt gacatataca
     4621 tcatctaatg atttcacatt ttcagagcga taaccgtagc ggataccatc gaatcgttgc
     4681 aaattggaac ttgcttctga tgaggcaatg atataataaa cagccacacc gtattttgaa
     4741 tgaggcaagc tgacttcttc cacaacagca cctaatgaac ggaatgtttc tgctgctttc
     4801 aatacagctt cttttacgcc aggggccaca ccttcgccta agtattcttt aggtaaagca
     4861 atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa aatcaggaac tgaaacgcct
     4921 gaagaagtac cgtctttttc gtcataaccg ctgatagctg tcaatgctaa tgcattatct
     4981 ttcaccgtac gggtcattgg tccgatttgg tcaagagatg aagcaaaggc aatcaaacca
     5041 aaacgtgaga cacggccata agtaggtttc atccctacga tgccgttaaa agctgctggt
     5101 tggcggatac ttcccccagt atcactacct aaagaaactg gaatttgtcc agctgcaact
     5161 gctgctgcag aaccaccaga agagccacca ggtacttttg ttgggtccca agcatttttt
     5221 gttttcttga aataagaagt ttctgtactt ccacccatag caaattcatc catgtttagc
     5281 ttaccaacag ggatcatatc tgcttgatag attttatcca tgaccgttgc atcataaatt
     5341 ggttcaaaat tgtaaaggat cttagatgca gcggttgtca agatatcttt tgttacgata
     5401 ttatccttga taccaattgg aataccagca aggacgttgc tttcatcaat ccctttttca
     5461 tctaaagctt ttgcctgttc caatgctttt tcttcgttca atgtaatgaa tgcctcgacc
     5521 tcaggctctg tttctttgat tcgttcaaat gttgtttttg tcaaatcttg tacagtgatt
     5581 tctttcgata caagcatatg atgcagttct tctagagaac gattgtgtaa ttctgtcatt
     5641 atgcgccagc ctccccatta tcgatgattg cgggtacttt gataaagccg tcttctgttt
     5701 ccggcacatt tttcaataat tcatcgcggt tccatccagc ttgcgcccga tcttgtcgca
     5761 tcacgtttac tgtttcaacc acattcgacg taaatggaac accttccgta tctacttctt
     5821 ccagcatttc aaccatatcg atgattttac ctaattggtc ggtaaaactt gcaagttctt
     5881 catctgcgaa cgcaagtttt gctaatttgg caacgtgttt cacttgttct tcactaatcg
     5941 ccatactacc ccttctttct aaacgagtta aaaactttgc tctctatttt aacacaagag
     6001 tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa actcattttt cattcttcta
     6061 ctgagcgact tttagccaat gcatctgcca tttgatcttc tgtccaaact tcgattccta
     6121 attcttgtgc tttggtcagc ttgctgccag catcttctcc tgctaccacg atatccgttt
     6181 ttttcgatac gctaccagtc actttcccgc caagattttc gatcgtctct tttgcttctt
     6241 cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt tttttcttta aaaggagact
     6301 cgacttcttg caattgggca tttcgaagcc ctttgtattc gaaattgacg cctgcttgtt
     6361 ttaattcatt catcaattca tggacttctt cgttactgaa ataagtgact acactgtcgg
     6421 caatgatatc accgatcgta tcaagcgcaa taatcgactc atagtcactt ttactcaatg
     6481 tttctagatc accgaaatgt tctgctaaaa tttttgctgc tttcgctccg acatgtcgga
     6541 ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt gctgttgtcg attgctgtca
     6601 aaatattatt tgctgatttt tcttttattt tgtccaacgt caataactct tcttctgtca
     6661 atttgtaaag atcagcaaca tctgctacta gttttttatc atacatctgt tctagtacgc
     6721 gcggccccaa cccatcgata ttcatcgcat ttcttgaaac aaagtggttc aaaccttctt
     6781 tcatttgagc agggcattta ggattgatgc accgtaaggc tacttcttca tccaaatgga
     6841 caagttcact tccacaaact ggacaatgtg taggcaattg gtattcttcg ctgtctttag
     6901 gtcgtttatc taacacgact tgcgaaactt ctgggatgat gtctcccgct ttataaatca
     6961 gaactgtatc tttcaagcgg atatccttca acttaatata atcaccgtta tgcaggctgg
     7021 cacggcttac tgtcgttcct gctactcgga ctggtgtcat gatagctgtt ggcgttacta
     7081 cccctgttcg accgactgtc cattcgatgt tttcgattag cgtttcaact tcttctggcg
     7141 gaaacttgta agctgctgcc catctcggtg ctttgatagt aaaaccaagt tgatcttgta
     7201 gcgagaattc attcactttg atgacgattc catcgatttc atagggcaaa tccacacgtt
     7261 tgtcatgata ttcttcaatg tatgaccaca cttcatcaat cgaatgacat agacgttttt
     7321 cccgattcgt gtggaatcca attttttcta gctcttctag cgcatcatac tgggtttttg
     7381 cttgcatagg accgaaatca gctactgtgt ataaaaaagt gtccagattt cttttcgctg
     7441 tgattttaga atctaactga cgcaaagaac cagctgcggc gttccgagga ttcgcaaaga
     7501 tatcttttcc ctctgcttct ctgtcttggt tcaattggac aaatgaccgt tttggcatga
     7561 aacattcgcc tcttacttcg atgttcattg gttcttttaa tttgattggg accgaccgga
     7621 cagtttttag gttttcagtg atgttttctc ctacagttcc atccccgcga gtcgcacctc
     7681 tcacgaaatt tccatcttca taacgcaaag aaacggaaag tccatctatt tttagttcac
     7741 agcaatagtc aactacacgt cccactgctt tttgtacacg ttgatcaaaa gctatcagct
     7801 cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat atcatgagtg actttttcaa
     7861 aaccttctaa tacttttccg cctactcgtt gagtgggaga atcagacgtg atcaaatcag
     7921 gatattgttc ctcgattgct actaattctg cgtattcttt atcataaaca tagtcttcca
     7981 ctgttggttt atcttctacg tagtattcgc gagaccattg gttcagtctt gtacgaagtt
     8041 cttctgctcg tttagcagct tcgtcaatcg tcatttcatg ttccatcatt taccctccac
     8101 tactaagctt tttcaattgg cgcaaaggca gccaaaagac gttttactcc ttgacttggg
     8161 aaagccacat ctagttctag atccttcgac gtcccgctta ctcggacgac tgttcctaga
     8221 ccccattttt tgtgtttgac tttatctcca ggtttccagc tgttattttc ccctccagta
     8281 gcacttttag agggaacaac cgctgtctct ggctgtttat agctatatgc tgttttttta
     8341 gcgaatacag gcgttttttt aggcgtagga cgcatgcctt cgatttccag taactcctga
     8401 tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg tccgtccata taatgttctg
     8461 gagaaagcat ttgttaaata caacgcttct tctgctcgcg tgatccccac atatgcgaga
     8521 cggcgttctt cctccagttc gctttcttcc atcaatgctc gagataaagg aaaaaccccc
     8581 tcttctagac caatcaggaa tacaactgga aattctaatc ctttggctgc atgcaaagtc
     8641 atcaatgtca cttgggaagc atcttcttct aaattgtcaa tgtcggaaac aagtgccaga
     8701 tcatttaaga agaccgttag tttttcttct ggtgcatcag cgtcttcttc attttgctgt
     8761 tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt cctccaagtt ttcgatgcgt
     8821 gcttgtgctt ctaatgtatt ttggattttt aagtcttcta gatatccgct acgatccaaa
     8881 acctctttcg tcaattctgt cactgtcaag tatgggatca tctgagtgac ttcttggatc
     8941 atctctccaa atgcacctaa ttgctgtcct gcttttccac cgatatttgc cagatccaca
     9001 ttttgcgctg cttcaagcag tggccattca tgaagagaag caaaactgcg tagtttttcg
     9061 atagatcctg gtccgatgcc tcgtttgggc gaattaacga tacgctcaaa actgattgaa
     9121 tcttgaggat ttaccagcac attcaaatac gctaaaatat ctttgatttc tttacgatcg
     9181 tagaacttgt gccctccaac cattttataa ggaatatttg ctttcaacag agtttcttcc
     9241 attacacgag attgtgcgtt cgtccggtac aagatggcaa agtcgccgta attccgatga
     9301 ttgcttcgaa tctcttcttg catccggtct acgataaaac gggtttcgtc tcgttcatta
     9361 tctgcacggt agtaagtgat cttgtttcct tctttgtttt ctgtccacaa gttttttggt
     9421 tttcgattgc tattgttttc aatgacttgg tttgctgcgc taaggatgtt tttcgtggag
     9481 cgatagtttt gttctagcaa aattactgca gcatctggat aatctttttc aaaatccaag
     9541 atgttctgca tatctgcacc gcgccagccg taaatacttt gatctgcatc tcccacgacg
     9601 cacaggttcc taaatctacc tgccaataaa ttgactagcg tatactgtgc gtgatttgta
     9661 tcttggtact catctacatg aatataatgg aatttgtttt gataatacgt gagtgaatca
     9721 ggatgttctt caaataatcg aatcgtattc atgatcaggt cgtcaaagtc catacattga
     9781 ttatattgaa gctctttttg gtaagcagca taacattttg ccgcaatctc ttcgtaataa
     9841 ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc tgttttttgc ttgagagatt
     9901 gttcctaata tacttcgagg atcatatttt ttcggatcaa tgttcagttc ttttagtatc
     9961 cgtttcatca aggtcagctg ttcagaagaa tcgatgattg taaagttccg gttatacccg
    10021 ataaaatcaa catctcttct taaaatacgc acacacatcg agtgaaaagt cgatacccag
    10081 acatcttcgc ctccagatgc gagaatcgca ttgacacgtt ctttcatttc ttttgcagcc
    10141 ttattggtaa aagtaatcgc taggatgttc caaggattga cttctttttc ctcaatcaaa
    10201 taggcaatgc ggtgtgtcag cactctcgtc tttccacttc ctgcacctgc cattagcagt
    10261 aatggtccgt cagtgtgaag aacagcttct ttctgacggg ggttcatccc gtttaataat
    10321 tcagctttgg atgacattaa caacatcctt tcttttatca atcttgttaa ttatagcatt
    10381 ttcttggctt tcattaaact agctagttca aaatcatttg tttcttttta tggatttttc
    10441 cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc gaggcgtttg aatggaaaaa
    10501 aaaggaaaaa tcaagaaatt agataagact cagatatatt ttattctaaa aggaatattt
    10561 gttggactgc tgacaggaat cgtcgttagc ctgtttcgat tatctatcga aaagctaagc
    10621 gatttagttc gaagtatcta tgaaatgagt cgggaacagc cgatttactt aattggccta
    10681 gctatttgct gcatccttgc agcatttttc gtgggttatc tggtcaaaaa tgaaccggat
    10741 atcaagggaa gcgggattcc acaagtagag ggacagcttc gcggggaatt atcaatgaac
    10801 tggttctctg ttttatggaa aaagttcatt ggcggtgtac tttcagttgg tgcgggcctt
    10861 tttctaggta gagaaggacc ttctatacag cttggcgcat ccattggaca aggagctgga
    10921 caattgtttc ggagtccatc ttcagaagaa aagattttaa tttctagcgg tgcaagtagc
    10981 ggacttgcag cagcattcaa tgctcctatc gcaggattac tctttgtttt agaagaaatc
    11041 catcactcct tttctccact tgtttggctg acatcatttg cttcagcaat cactgctaac
    11101 ttcgtctctc tttacttttt cggcttgaga cctgttttat atctaggtga ccttccttct
    11161 ttgccgttga aatattatgg ttccttagtg atcttaggag tacttctagg catcttaggt
    11221 tttatttatc agaatgtctt gctggctttg ccaaaatttt acaaccgatt gcctttacca
    11281 gcttattttc acgggttcgt tcctttcttg ctgattttgc caattggtta tttatggcct
    11341 agtactctag gcggaggcaa tcaaatcgtt ttagcttttg gacatgcttc cttgccaatc
    11401 attatgatta tcggtttgtt tgttcttcga ttcgtttttt ccatgatttc ttacggtggg
    11461 ggtcttccag gggggatttt ccttccgata ttgacattgg gggctttaat cggtagcttt
    11521 tatggaaaca ttatggttga tttcgggatg gatccaatct acatcaagga ttttgtcgtt
    11581 attgccatgg ctggttattt cacagcgatt ggaaaagcac cgctgacagc aattatttta
    11641 gtgacagaaa tggttggctc tcttaatcat ctgatgcctt taggattggc tgctcttgtt
    11701 gcttatatcg tcaatgacct tctcggcggt aatccaattt acgaatcatt attggaacgt
    11761 ctacttggaa atactcagac cgcctcacta aaaggtgcaa ggacaacttt tgattttcca
    11821 gtaacggcag agagttcttt agacggatta atggtacgag acttcaattg gcctaaaaat
    11881 atgctgctaa tctctatacg cagaggaaat caggaaatct tgactcacgg tgatacgatc
    11941 atgaatgtag gagatatttt ggttatcttg actgatgaag caaacttgcc aagaatcaag
    12001 caggaaatcg aggctaaatc ttctatcaca aatttgaagc aatcatttta acgaacatgg
    12061 aaaaactatt ttacaacaac tattcatcta cactacttga ttctatatga ttctataaaa
    12121 aaagaagagg ctgggaaaac ccagcctctt cttttttatt tctatgcttc tgctactgca
    12181 ggagcttttc tcaataaacc ataaatcaca ccagatacga ctgctccgat agcaataaac
    12241 accaaataca tgaccgggtg actcagtaag aatacaacga aaatcccgcc gtgaggagcc
    12301 agtaatttga ttccgaaagc tccgactaat ccgccagtta aagcagaacc aataataaaa
    12361 cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa tcgatccttc cgtgacaaac
    12421 gatagtccca tcacgatgtt cgtcaaacca gcatcttgat ctgttttgct gaatttattc
    12481 ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta ccattcctcc agccataaca
    12541 gaagccatca cgacacttcc gccagttgca acagtcgtag ctaaagttgc tgtaccaaac
    12601 acataagcgg ctttgttgat tggaccgcct aaatctgccg ccatcattcc agctaataat
    12661 gcaccaagta aagcagcatt cgaaccactc atacttgtca agaaaccgtt caatgcatca
    12721 ttgattgctt tcatagggac attgaccagc aacatcaaga aaccagtaat cagtaagcca
    12781 aacactggat aaaagaggat cgttttaatt ccatccaatg attttggtaa gttcttgaat
    12841 aatttacgta aaaatacgat gacataacca gctaagaatc caccaatcag tgcacctaag
    12901 aatcctgcac cgcctgtatt agctaatgca ccagcagcaa atccaacgat caaaccaggg
    12961 cggtcaccga tacttgaagc aataaatcct gctaataccg gcagcatgaa accaaatgca
    13021 gcttgtccga tttggttgaa ccagctagca gcttgattgt aattccctag atttgataat
    13081 tgatcatgtg gcacgccaat aaattggtcg atcatgaaag aaagcgcaat agcgattcct
    13141 ccaccaataa cgaaaggtaa catgtgtgat acgccgttca tcaaatcttt gtaaattctt
    13201 tgtccgatag taccatccgc atttccttct tctgaactgc tttcttttcc atcaccatgg
    13261 aatactggtg cagtaccact aagggctaga ttgatcaact cttctggttt acggatacca
    13321 tcactgacag gacgattgat cagttctttt ccgtcaaagc gattcatctc tacttttttg
    13381 tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt cagcagtcaa tcggtttttg
    13441 attccttctg aaccgttcgt ttcgactttg atctccacac ccatttcttt cgcttttttc
    13501 ttcaaagcat cttctgccat gtaagtgtga gcgatccctg ttggacaagc tgtaacggca
    13561 acaacgaatt ttctttctgg atgagcagaa gcttctgcag cttgtgtttc ttgttctgct
    13621 ttttcttctg cttctttttg ctcttcagct gtttggaaca agtcttggac ttcttctggt
    13681 gtatttgctt cttttaattt tcctacgaaa tctgcatcaa tcagcaatcg agatagtgct
    13741 gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag ctgcgatcat gaaaaacaga
    13801 taagttggct gtccgtctaa ggcttcataa tctactcctt tttggctttt tgcgaataaa
    13861 actgttgctt ctttaactgc actgtttttc gcgtgaggca tggcgattcc atcaccaaga
    13921 cctgtagaag tttgtgcttc gcgggctaga attccttctt tatatgtttc gatatcggaa
    13981 atccggccgg catcaaacat tttttggacc atttcatcga ttgcgccttt tttatcagtc
    14041 gcttgcaaat ccatgatcat cgcttctttg accattaaat ccttgatatt catgattatc
    14101 ctccttgaaa ctaatcaatt tttgtgattt caacttctgg cagcaactca tcaatcaatt
    14161 cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc gcaagcaact ccccatttaa
    14221 aggcttcgat tggatctttc gtacggctgt aagccccaat aaagccagca atcattgaat
    14281 cccctgcacc tactgaattt ttcaatgggc gaactaatac attcgtttta taagttcctt
    14341 gttccgtgaa gagcaaagca ccgtctccag ccattgagat cagcacattc tgtgcccctt
    14401 cagctagtaa ttttttccca tacggaatga tttcttctac agattggaag gttgtgttgt
    14461 aaagctcagc taattcgtga ttgtttggtt tgatcaacaa cggtttctta acaagtgcat
    14521 tttttaggtc atcacctgtt gtatcaatca cgaaatctgc acctttttct cgaatgatat
    14581 caatcagttc ttgataaaat ccttttcgca ggctagctgg cgcgcttcct gacaatacga
    14641 cgatatctcc ttgtcctact gttcctaatg cttgcttcaa ctcatcgatt tcttcatctg
    14701 aaatagatgg gcctaagcca ttgatctcag tttcagtgtc tgattttagt ttgatattga
    14761 ttcttgtatc ttcttttacg gttgtgaaca aagactggat ttgttctttt tcaagccact
    14821 tcgaaataaa ttcgccagtg aaccctccta aaaaccctag tgctgtagat tgagaatcga
    14881 tccgtttcaa aatacgggaa acattgatcc cttttcctcc aggtaacttg aagtcttcct
    14941 tcattcggtt caaatcgcct agttttaacc catctacgcg tacgatataa tcaattgatg
    15001 gattaagtgt tactgtgtaa atcattgtat tgcctcctta attgttgtct tttcacggaa
    15061 ttcattaacg aattctaatg gacaacgctc ggttatgatc gtggctgctt ccaaaggtgc
    15121 aacagaggtg aaggacaact ggttgaattt ggtatgatca atcaatacaa atgtctcttc
    15181 ggaatttttc atggctaaac gtttaacagc tgcctcttct ggatccggag tcgtgaatcc
    15241 ttgttcaaga tgggcaccgt tcattcccat aaatgcttta ttaaaatgaa aattttttag
    15301 ttgttctatc gcattagatc ctaaaacagc attagttgcc agctttatcg ttccacctaa
    15361 aataatggta gcaagctcca aatcaactaa atttgccgca tgttgaacgg aatttgtcac
    15421 tactgtgatc tgttttcctt gcagaaaagg aatcatttct aacgtagtag agccagcgtc
    15481 taaataaatg acatcttcgt cattaatggt ttcagctgcc gatttggcga tttgctgttt
    15541 ttcgtgaacg tttttgagtg atttttcact catattctgc tcaaaattca ataattgctc
    15601 tttcttagct ccgccatgaa ctctttctag aaacccacta ttctccagct cttgtaaatc
    15661 acgtcttatc gttgattcag aagcattcaa aaggtttaca agatcttgtg atttcacgat
    15721 tttctgctgt tcaagcaagt ctaggatttt ttgtctgcgt tcctctgtaa gcattttcac
    15781 gatcacctca atacaaataa tagcatattt tttttctact agcaatataa atcagtcaaa
    15841 aacgttcata aaaattataa atcagtcaaa aaaaaataaa aaaccagtca gaaagactgg
    15901 ttttttattt ttttgtctat catcaagccg ttttatcgtt ttttattttc gcttcttgtt
    15961 tttttcatat aaacagaagc aggttttctt tccttatgaa gaagataata ataaagtccc
    16021 atcccgccaa acatgaccaa aaaaccgatg actgatcgaa taggaaaatt aggaacaaaa
    16081 aagagacaga cggataataa tgatgccgga accattattc gataatagag ttgtttatcc
    16141 attttcttca cctcatattt tttgtaaagt aatcaattgt tctaattctc ggtaatctaa
    16201 aaattgcaca cgcagatgct gctcaccttt cttgacatcg aattggatac ttcgccgctt
    16261 tttctttatt cgtttgaagg taacatcaga gagtctatgt ttgatttcct ttaacggtaa
    16321 ttctgacagg gattctgaag gaacatagat tcttaaggat aaaagatact cttgatattt
    16381 ttcatctgtt acctctactt ctagatattc cctaatataa taacaataag cttcgattcc
    16441 attatccaaa gcaacagtcg gatcgataac tttatagcac atcatgcgtg atcgattctc
    16501 gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg cgaacacgcc tttctttata
    16561 atctccttgt tggatatcat aactttttgt tacaaaaaat gaagcattct ttttaggaac
    16621 aggttgcatt tgtgcattct gatactggac agattggatc gtatggtcag tgtaaatgta
    16681 ttcaacgaat ggcggtttca ataaaccgac accattgtcc caagcaagat gatcaaaccc
    16741 aagctggtcc ctaaaatagg attgaaacat ttgattgttt ggcttttgcc agttaaaggg
    16801 gtctcgataa agctggatac atatgacttc gttttctaat tccttcactt tttctacttg
    16861 ttggatgtcg agaaaaatcg gtttagggat gatttgatga taatagcgac tataatacat
    16921 ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga tcgtatcctg gaaattggtt
    16981 gcaatcgact actacttccc cgttttgttg tttcatttta ggtaaatgtt cgatttcatc
    17041 ctttttctca aacaatagcc gttgagagat ctgttttgta ttgtgataga taaactcgct
    17101 ataagctcgc atataggcga acaccccatg agcaatcatt tttttctgaa tgaagtctcg
    17161 aatcaaaaat tgcttttctt tgaaaacaga gatagacaat acatttttta tcaataattg
    17221 attatcttcc aagcgaaaac tacattcgtt ctcgtcattt cctacaacta accaactttc
    17281 gtctcttacc gatgctagca ttattttttc ttgaagcttt cgaagatcat acggatccgc
    17341 ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac agacgtgtca aaatagccca
    17401 atcgcttatt tcctgttttt tttcttcttc atttaagtct gtgagctgga tagtcatcaa
    17461 taatttatct tcgccaatca ttttctcacc tctttcaaat ctttttataa tttaattata
    17521 accaatgata aagaaaaact aaaactttat gtcttagaaa ccaatttgga cgattacatg
    17581 atagactaag taaaaagaaa caaagaggtg tttacaatgg ggttgaaatt tgaacgtaca
    17641 gataatttag ataaactttt cgaatcattt gctgtcgatc cggaaaaaac agttgcacca
    17701 aaagaagaga tcgatcgatt tctaaaacca cagccacaaa atgaaaagaa aaaagacaag
    17761 aaacaaaagt aagttaagct caaaaaaaga cttaccagcc aaacttttat gaaagagttt
    17821 gactggtaag tcttttttat tctttttatg attcaattgc ttgttcgatt gctgcccacg
    17881 cttcttcttt tccctgtttt gtaacggaag aaaagagaat aaacgtgtca ttgcgatcaa
    17941 aatccaacgc ttttttgatt tcgctctcat gcttgttcca tttaccacga gggatcttat
    18001 ctgccttagt ggcaacaaca attacaggga tatcataata cttcaaaaat tgatacatct
    18061 ggatatcttc ttgagaaggc ttatgacgca aatctactaa agaaacgacc gcacgtagct
    18121 ggtctctagt cgttaaatat gtctcgatca tctttcccca ttttgctcgc tcagttttgg
    18181 atacttttgc atacccatat cccggaacat cgacaaaatg taatgcgttc tcgatcaaat
    18241 aaaaattcaa tgtctgggtc tttcctggct taccggaagt acgggctaaa ttctttcgat
    18301 caatcaatgt gttgataaaa gaagatttcc ctacatttga tcgtcctgcc agagctattt
    18361 ctggcaattg tgtatctgga tattgttttg gtgccactgc acttatgata atttcagctt
    18421 gatggacttt catggtctct ctccttaaca aggtgatgaa taaagcaagc ggctgtcgca
    18481 aaagccaaaa tgacttttac ggcagcctct tttcatcaac ccgctttttt ctctgaatct
    18541 ttatagatta ctgttggctc accagacaat tcagctgctt cttttgtgat aatcactttt
    18601 tcgatgtttt catttgaagg gatatcaaac atcacatcca tcatgatttc ttcaatgatg
    18661 gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt ttttcgcaat ggctctcaac
    18721 gcttcaggtt cgaattcaag ttcggtatta tctagagaaa gaagtttttg atattgtttg
    18781 actagcgcat ttttcggctc agtcaagatc cgtaccagat catctgttgt caatttctct
    18841 aaagctgcca taacaggtaa tcgtccgata aactcaggga tcaatccgaa ttttagtaaa
    18901 tcttcaggaa tgatatgctg catgacactt tcgtcttcag aaagtttttg gttttgtgta
    18961 ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa tcgtttcgat gccatcaaat
    19021 gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct gaataaattc ttgatgtgga
    19081 tgtttacgtc cgccctgagg tggcacactt gcaacagttc cttctagaat tttcagcaag
    19141 gcttgttgga caccttcgcc ggaaacgtca cgagtaatag atacattttc gcttttacgc
    19201 gcaattttat cgatctcatc aatatagatg atcccttttt ctgcaagttc cacattgtaa
    19261 tcagcagatt gaagcaattt aagcaaaatg ttttctacat cttcccccac ataaccagct
    19321 tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat tcaatgtttt tgctaatgtt
    19381 tgtgctaaaa atgttttacc agaccctgta ggtccgatca agcagatatt gcttttttgc
    19441 agttcaacat catctgtgtt ttcttgtgct tgctgattga cacgtttata gtgattgtat
    19501 acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa tcacgtagtt attaaatact
    19561 tcaagaattt ctagtggttt agggacttca gtgaattcgc gtacagcttc ttcataaaat
    19621 tcttcatcaa tgatttcttt acataggtcg atacattcat tacagatgta gacccctgga
    19681 ccagctacga tctttttcac ttcttcttgg gttttcccgc aaaaagaaca gcgtacggtc
    19741 tcattgctac ttggattgtc atacatggtc ttcacccctt aacttgcaaa aagtatgcaa
    19801 gaaactatca aagactttac agtcttttac tatcataaca tagtttagta aaaagaaaaa
    19861 gtgtttaaca aaggatcaac tgacctatac ctttgcattt tttactctta acaaaaaata
    19921 aggaactgtg acaaaatcgt gtcacagtcc cttatttcga ataaacaatt ttctagaagt
    19981 agcttcttca gaaatgattt attacttgat ttctattctt tcgcttctgt cgctgtacct
    20041 gtgatcaatt cgattgcttt tttcattgtc acatcatgtt ccagcatatc ttctgtcaac
    20101 acgcgtttga tttgttcgat tggcatgttg tatgtttctg ataattcttg gatttctgca
    20161 ttgatttctt cttctgttgc ttgaagattt tcagcagctg caattgcttc gatcacaaga
    20221 tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt tatgcaagtc ttcttctgta
    20281 gaaccagtta attgatagta catttcagga gagatacctt gacgttgcat gttgttcaag
    20341 aattcgtcca ttgaacgatg tacttcatca tgaaccatca cgtgtggcaa ttcaacgatt
    20401 tctgcgtttt caacagccat acggatcgct gcttcatctt ttgcatcgtc cgctgctttt
    20461 tcttttgttt ctactaattc tttgcgatat ttttctttca attcgtctaa tgttgatact
    20521 tcgtcatcaa catcttttgc gaattcgtca tctaattcag gcacttcttt tgcttttact
    20581 tcatggattg tcactttgaa gactgcttct tttccagcaa gatcttctgc ttgataatct
    20641 tctgggaaag ttacatttac atctagactt tcacctgatt tatgaccaac taattgatct
    20701 tcaaaacctg gaatgaatga gcctgaacct aattcaagtg agtaattttc gccttttccg
    20761 ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga ttactactgt atcgccattt
    20821 tcagctgcag catcttcttt gataactaat tcagcttgtg cttcttgttc acgtttgata
    20881 cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt tttcaacaac tagatcttta
    20941 tattgtccta attttacttc tggtttcact gttacttcag cagtgattac ccagtcttga
    21001 cctttttcca tgctttcaac atcgattttt ggttgagaaa caggatcgat gccagcttct
    21061 ttgacagcag cttcataagc ttctggtaat acagcattca atgcatcttc atataaagct
    21121 tcttcaccgt acatacggtt gaatacttga cgagaaactt tccctttacg gaatccaggt
    21181 acgttcaagc tttttttcac tttattaaac gcagttgtta atcctttttg gatttcgtct
    21241 tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt ttttttcaaa tgttgcagtc
    21301 atttaattcc ctccgagttt agaaattcat tttatacatc aaaaacgttt catgcatacc
    21361 tttaaatagt acactaaccc tttcaaattg taaacatatt gaatggatag aatccttgtt
    21421 tttcataaca tttttatctt tttcactgat aaaatctaat ggaagaagat catttgctgc
    21481 aaaatttttt ctcttttttt taaaaaatct tgtttttcat cttctgtacc ttcatacatc
    21541 gtttttcctg tacacgcctc tacagtcagt tgaaaccagc gcttcgggtc ttcgatgtat
    21601 gtatcataga caggataaac aattgcagat tgcaacgtga attcttcttt taacacggaa
    21661 ctaagcagcg catcttcatt cccgtaacga tctgaaaata cacataattg ctggtaactt
    21721 gcctgctgct gcggctttgg cagatctttt ggtatgactt tgactaactt ttcttcaatc
    21781 gtcaaataga aaacaggatg gcattcgttc aattgcctaa gtgtctccag taattttgcc
    21841 cttgctaaaa tatggacctc tggcgataca ataagtttac tcgctgtttg gataaactca
    21901 tgataaggaa gctgttcgat ctcatcaata gacgcaagct gacgtgcagg ttccaacgaa
    21961 ggaagttcct ccgatttatt gtggagtgcc ttgatcttct gcctctccat ttgtccggat
    22021 aattcttctg caaattggat tttttcaatg acccgctgct cgaaagcttc ataaaaatct
    22081 gtacttgcta tcaattttct agcatataaa aaatcttgtg tactgattaa caagtcaaaa
    22141 taaaattcag caaactcctc attttgaaga tactgttttt catgcaagag tgcttgcttc
    22201 aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa caagcaggct attagcctca
    22261 aagctttctt tcaactcgta agctcgttga aataattctt ttgcctgctg atattgctgg
    22321 tttgctagtg cttgttgtgc cactcgcatt aaattctcat atttttctgg aaaatcaatc
    22381 ggcttcacgc agtgtcctcc tcttgctcat attcagctca tgttcaatag tagcaaaaca
    22441 aaaaaagcat gacaattaaa aataactgaa gcaccctcta aaagctagat tttagatctg
    22501 acttttagaa ggtgctttca actgttactg ctttttattg ctcatcagct gttgacttcc
    22561 tggttgaacg ctttgatcag attgatcgta acaacacttg tattagtgaa aaaatcgccc
    22621 agatgttgct ttttctgatt aaagactgct ggaagatacc ctaacatcag caaaggaaat
    22681 tttagaatga accggcaagc accttctcta actaaaacgg ttgaccagct aagttcttct
    22741 tcatcaaagc tgatgactcg aataccaaag atcattttcc caatcgtttg gccatgattg
    22801 aatttcgtca ataaaataaa atacgctaga taaataagta aggctgtgaa tccgtagaga
    22861 ctcagataag aatctgaaaa attaagccaa cctagatttt tggcaatacc aagtgttaaa
    22921 cgcgtaatag ctccgataca caaaagatcg accaagaagg caaacattcg gataaaaaaa
    22981 cctgcataga aatacttagg aaaatcattt gcttttggcg gttcatttct tgtgtccgta
    23041 tactttttcc atttacgttg tttctttttg atttcttcgg gcgtcaaagg ctctctttca
    23101 aaagagctca gtaccttttg ggaaaagttg gagtcaggtc ctacagcagg ctgctgttgc
    23161 ttatcttttt cttgttcctt attttcattt gaaagctctg acatgcttac tcacctccgt
    23221 aataatacat tgcttttggt gattccgctg tccctagatt ctcaagaacg gataagatct
    23281 gactagtctc agaaggcttc aatccctgga attctgctag tttacttcct aaccatgtac
    23341 tagcaaaccc agatggcgtg gaagaatatt ctactagttc cgcatcttca agctgttgtt
    23401 cctttcgtat agcttttaaa gcatcttcag gaaatcccaa ttcatcgact aaacctactt
    23461 cttttgcttg gacaccatca taaatccttc catcagcaat ttttttgact tcttcttcag
    23521 atttattccg tccctcgctg acgattgcta caaaacgact atatgcgctg tcgatatatg
    23581 cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt tcctgaaccc atatctttca
    23641 acgctccgct tttaactgtt gtatcttcca cacccagttt ttctagaagt cctgaataat
    23701 tcaatcctga catgatcaca ccaattgagc cggtcactgt ttcttctgtt gcaaagattt
    23761 tatctgcttg agcagaaata tagtagccac cgctagctgc catgtttttc atgctgacat
    23821 acattgggat atcatgttct ttacgaatcg tatccaattt cttagcgatt tccgcacttt
    23881 cgtagacacc gccaccagga gaatttactt ctaaaaagat acctttgact gttggatctt
    23941 cttcaatcgc cttcagttgt tccataaaat ttttatgatt gtaaccttca gaagcaaata
    24001 aacctgaaga ccctcccgca gcaatcgttc catctactga taattttacg attttttgcg
    24061 tactgtctcc ctcttccaac acatttggag ctagttcttc atccccatat aaccaactgt
    24121 tcaatccctc aatttgatcg ttttcctgag gcttagtcaa actagctgaa actaacgaaa
    24181 ccacaaataa tcctgcagca atcagaactg ctgcccaacg tcttttattc attctttctc
    24241 ctttctaata caagtttttt atgacgatca cagtttctga ctgctcagaa gtgtcgaacc
    24301 gctctgcttt ttcttcccgc gcatacccgg aaaacgactc ttttgttaat tcatcttcta
    24361 ccgtaaactc acgagcttga gcaacttcca aggcatcagg tgcgatttct tggatgctat
    24421 cccagccagt atctaaaaat attttcgtat catctgatac accagctgat gcttcgaatt
    24481 tggacaattg tttttttaac attcccagtg tcatgatctc aatttttgaa tccattttta
    24541 ctcctcctct atccgattac tttctattgt accgaactcc tgaaaaagat gatagataaa
    24601 aacccattat tttttgacat aaaaaggaca tttgacaaag aatttgatat ataccaattt
    24661 attttttatt ctttttttgt tgacaagaac attcgcaaaa ggttatgatt ttaaatgttt
    24721 ccagataaaa aacaaaaagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atgtctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgat gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaac ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagatgct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actttggaaa aattgacaca gattcctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagacagcc gccaagtgct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggtgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgatcggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttacgcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccgtcat tgatatttaa
    27541 cccgtctgga tttgtgccca ttgtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaactt catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatc
    29221 ggcacccccg taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatt agttctctac
    29461 gcaagattgc caaatattct tgctgggata agccagccat cagttcatta tctaacgtga
    29521 tgctgacgtc cccggtttct tcagagacaa cgatggtaat cgcgtcactg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attctttagg gatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc cccagtgata tctgcatcta
    29761 aagcaatccc tgtctcaata tactcatcta atccagtatg acgttcgata gtgatcaacg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tatgctcgta tttttcttga gaaaaacgca taattttcaa
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct agcagctctt ctttcttctg cttcaaggtc
    30541 cgtcgttttt tcttttctag tcttaacggg tacatcgata gattcctctg gttcaagtcg
    30601 tttcttgatt ttttctaatt ctcccgtcag tgcagcatag ttgtaaaaac gtgttttagc
    30661 gtcgtggaca gtcttttcaa ataaagcttt tgcaagattt ggatttgcgt tatctagcga
    30721 ggaaaagcga acttgtttcc tcatgaaatc aggcatagca gagaaatcag gttttttgta
    30781 gtccaaagtc attgggttct tgtttttttc gaccattgta gggttatatc gatataatga
    30841 ccaataacca gaagaaacag cttcttttgc ttctttaagc gattctttca ttccgccaag
    30901 caagccatgt gtgatacaag gcgtataggc aataatcaga gaaggaccag gatacttttc
    30961 tgcttcttca aatgccttga tcgtttgcat ttgattggca cctgaagcaa tttgcgccac
    31021 ataaacattt ccatatgtca tcgccatcat tcctagatct ttctttgaag catactttcc
    31081 actagccgaa aatttagcga ttgccgaagc tggtgttgct tttgaggttt gaccgccagt
    31141 attggaatag acttcgttat ccaaaacaag gatattcacg tctgctccgc tggcaagtac
    31201 atgatcgatc ccgccaaaac caatgtcgta agcccatccg tctccaccaa tcatccattg
    31261 gctgtttttt acgaatagat cctttctctt gtatatatcc tccagttcag ggaaaattga
    31321 tcgttcgctt tcgatcgcag cttgcagttt tgctgctctt tgctgcgttc cttcactctc
    31381 aaacatgtgt actaaccagt cttccatcaa ttctcgtagt tcttctgata cttttggcaa
    31441 tacttctttg attgaacttg ccaaagcctc gcgacgagtc tgtgccgcta gaagcatacc
    31501 ataaccaaat tcagcattat cttccaataa agaatttgac catgcaggcc cttgaccaca
    31561 ttcattggtc gtataaggag aagcaggaga agcaccgccc caaatagaag aacagcctgt
    31621 cgcattcgcg atcatcattc gatcaccgaa catttgtgtc aataatttta cataaggtgt
    31681 ttcgccacag cctgagcaag cgcccgagaa ttcaagaagc ggttgattga attgtgtaga
    31741 caagacagta ttctttcctt tgactggatt tgctttttgt cttaatgtca ttgcaaaagc
    31801 ccaattcaca gcttgttctt tctgttcttc ataaggcttc atcactaaag ctttttcttt
    31861 tgcgggacaa gcctctacac acagaccaca acctgtacag tcttccactg aaacttggat
    31921 acggtatttt acgccgtctg cgcctcgcat ttctctcacg ataaatcctt ctggcgcttc
    31981 ttccatttcc tcttcatctg ctaaaaatgg acggatggca gcatgaggac aaacaaaggc
    32041 acattcatta cacatcgtac agcgatcact aatccactca ggtacttcta aagcgacgcc
    32101 ccgcttttcc atagaagccg ttcctaaagg catttcgcca gttgtcattc catttgctac
    32161 aagtgtacca acagagagtt gatttccttc ctgtgcattc actggttcaa gtatttcttg
    32221 tatataacca gtgttccttt tattgatttt ttcaggcatc tccaacgttc tccaatggtc
    32281 tgggatcgcg acttgatgaa gcagttcggt cgtccgatcg attgcttgta tgtttttttc
    32341 tacgatagcc attgatttat gtccatagct tttcagggct tcttctttta gcagcggaag
    32401 cacctcatca aaaggaatga tgtcagctaa tttaaaaaaa gccgtttcca ttgccgtatt
    32461 gatccggcgt cccaaaccga cttcttgggc caatctcatg gcattgatcg tatagaatct
    32521 aatgttattt tcagccaagt aacgttttaa ttttaagggc agatgcgttt ctaattgttc
    32581 gtcgctccaa agagtattca ataaaaaaat gccacctggc ttcaatcctt tgaccaagtc
    32641 atagctatgc aggtaagctg gtgtgtgaca ggcaacaagg tccgcatgct cgacaagata
    32701 cgctgaacgt atcggcgtat ctccaaaacg aagatgagag acagtcagac cgccagattt
    32761 tttcgaatca taataaaaat agccttgcgc atatttatcg gtgtgatctc caataatctt
    32821 gatagctgat ttatttgctc caactgttcc gtcagatcca aatccccaaa acttggcctg
    32881 gaatgtttgc ggttctgtca aatccagact ttcctttggt tccaaagatt gataagtcac
    32941 atcatccacg atccctatgg taaaccgttt tttgcgtatg cttgggtctt ttttcaattc
    33001 atcaaaaact gctgagattt ggtcgggagt cacatcttta gaccccaatc cgtagcgacc
    33061 accaataaca gccgggcgaa gttcagaatc atataacgca ctttgaacgt ctaataagag
    33121 aggttctcca cctgcccctg gttctttact tcgatcaaga acagcaattg tttttactgt
    33181 atgaggcaac ttttgcacga atgtttccac tggaaaagga cgataaagat ggatattcaa
    33241 aaaacctgtt ttccgtccgt ttgcattcaa gtaatcaatc gtttgttcaa tcgtctgtgc
    33301 aactgatccc atagaaacaa tgacttcttc cgcatcgtct gctccgtaat aagtcaccaa
    33361 gtcataagaa gttcctcgta atcggttgat ctctttcata tatttttgaa cgatttcagg
    33421 taatttatca taataggggt tgatcgtttc ccgttgttga aaatggatgt ctggattctg
    33481 gttcgtacca ctaacagtcg gatgattggg attcatccct cgtttacgaa atgaagctaa
    33541 tttctcctga ttgatcagag gagctaattc ttcatattcc aaaacttcga ttttttgaat
    33601 ctcgtgactc gtacggaatc catcaaagaa attcaaaaaa gggacttctc cttcaataga
    33661 agcaagatgg gcaacaggtg ctaaatccat cacttcttgt acactgcttt cagctaacat
    33721 agcaaatcct gtttgtcttg cagccatcac gtctccgtga tctccgaaaa tattcaacgc
    33781 atttgtcgtc actgcccgac tagcaacgtg gaaaacactt ggcagtagtt ccccagcaat
    33841 cttgtacata ttcggtatca tcaaaagcag cccttgtgaa gcggtatatg tagttgtcag
    33901 tgcaccagtt ttcaatgatc catggaccac accagctgct cctgcttcgg attgcatttc
    33961 tactactttt actggttgac cgaatacgtt ttcttttcct gctgctgacc attgatctac
    34021 taattcagcc atggtcgaac tgggtgttat tggatatatt gcagcaatct cggtaaatgc
    34081 ataagaaata tatgcggctg ccgtattgcc atccattgtt ttcatttttc gcatgattca
    34141 tcatccttgt tcatattctc tcttattatc tgttccatta taacgtaaaa catgccattt
    34201 ttttctttta tttataaaaa tattttacat gtatctattt gaattggtca tctaattttt
    34261 agacaaacag tctaaatcgt tgcctttttt tgtcagacat gtataaacta taagcgaaag
    34321 aggtgtttta acatggatat atcagtgatt gatgcaacga aaacaaatac acaaaaagga
    34381 atcctttatg gttcatcgaa tgctcctaaa aaaatggtcg aattcatcaa cttagcttgt
    34441 ccgtattgcc gacaatggtt tgaagaatct tatgagctat tagaagaagc agtccaaagc
    34501 gggcaattgc agcgagtgat caaattgttc gataaagaaa aagaaagctt acagcgaggg
    34561 aacgtgatgc accggtatct cacaatcagc gatggccaaa aagcgatcaa agagatcaaa
    34621 caaatattcg atactcaaga cgagtggaaa catctttctc tccaagaagt agcggatttt
    34681 gcagtagata aactaaagct gacagaacaa aaagacgaac agctgagcca agctgtcatc
    34741 aacgaagctg agcaagcaca tattcgattt gttccaactg tcatcttggg taaagaaatc
    34801 tttgatgaat ctatttcaat agaggaatta aaagaattga tccaaacaaa ataaaaaaat
    34861 aaggagcgtg ggacaaaagt aa
//
LOCUS       DARBER010000016        34790 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARBER010000016 DARBER010000000
VERSION     DARBER010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.016"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="SCK06_000800"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="SCK06_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG1150824.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="SCK06_000801"
     CDS             complement(2020..2922)
                     /locus_tag="SCK06_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1150825.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="SCK06_000802"
     CDS             complement(2919..3611)
                     /locus_tag="SCK06_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG1150826.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="SCK06_000803"
     CDS             complement(3654..4295)
                     /locus_tag="SCK06_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1150827.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4602)
                     /locus_tag="SCK06_000804"
     CDS             complement(4333..4602)
                     /locus_tag="SCK06_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150828.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4575..5264)
                     /locus_tag="SCK06_000805"
     CDS             complement(4575..5264)
                     /locus_tag="SCK06_000805"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG1150829.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="SCK06_000806"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="SCK06_000806"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG1150830.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="SCK06_000807"
     CDS             complement(5984..6916)
                     /locus_tag="SCK06_000807"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG1150831.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="SCK06_000808"
     CDS             complement(6913..7689)
                     /locus_tag="SCK06_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG1150832.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="SCK06_000809"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="SCK06_000809"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG1150833.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="SCK06_000810"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="SCK06_000810"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG1150834.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="SCK06_000811"
     CDS             complement(12014..13294)
                     /locus_tag="SCK06_000811"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG1150835.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="SCK06_000812"
     CDS             complement(13313..14239)
                     /locus_tag="SCK06_000812"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG1150836.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="SCK06_000813"
     CDS             complement(14252..15535)
                     /locus_tag="SCK06_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG1150837.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="SCK06_000814"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="SCK06_000814"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG1150838.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="SCK06_000815"
     CDS             complement(16323..17234)
                     /locus_tag="SCK06_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1150839.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="SCK06_000816"
     CDS             complement(17231..17695)
                     /locus_tag="SCK06_000816"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG1150840.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="SCK06_000817"
     CDS             complement(17700..18398)
                     /locus_tag="SCK06_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1150841.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="SCK06_000818"
     CDS             complement(18385..18771)
                     /locus_tag="SCK06_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150842.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="SCK06_000819"
     CDS             complement(18918..20588)
                     /locus_tag="SCK06_000819"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG1150843.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="SCK06_000820"
     CDS             20771..21004
                     /locus_tag="SCK06_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEG1150844.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="SCK06_000821"
     CDS             complement(21103..21534)
                     /locus_tag="SCK06_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG1150845.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="SCK06_000822"
     CDS             21613..22026
                     /locus_tag="SCK06_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG1150846.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="SCK06_000823"
     CDS             complement(22064..23212)
                     /locus_tag="SCK06_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1150847.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="SCK06_000824"
     CDS             complement(23334..23930)
                     /locus_tag="SCK06_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1150848.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="SCK06_000825"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="SCK06_000825"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG1150849.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="SCK06_000826"
     CDS             27160..27591
                     /locus_tag="SCK06_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1150850.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="SCK06_000827"
     CDS             complement(27636..28985)
                     /locus_tag="SCK06_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1150851.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="SCK06_000828"
     CDS             complement(29055..30218)
                     /locus_tag="SCK06_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1150852.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="SCK06_000829"
     CDS             30347..30670
                     /locus_tag="SCK06_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150853.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="SCK06_000830"
     CDS             complement(30853..32202)
                     /locus_tag="SCK06_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1150854.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="SCK06_000831"
     CDS             complement(32271..33224)
                     /locus_tag="SCK06_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG1150855.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="SCK06_000832"
     CDS             complement(33326..34516)
                     /locus_tag="SCK06_000832"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG1150856.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9892 a         7615 c         6102 g        11181 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaat ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt
//
LOCUS       DARBER010000017        34572 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARBER010000017 DARBER010000000
VERSION     DARBER010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.017"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..2135)
                     /locus_tag="SCK06_000833"
     CDS             complement(243..2135)
                     /locus_tag="SCK06_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150857.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2132..3859)
                     /locus_tag="SCK06_000834"
     CDS             complement(2132..3859)
                     /locus_tag="SCK06_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150858.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3875..4525)
                     /locus_tag="SCK06_000835"
     CDS             complement(3875..4525)
                     /locus_tag="SCK06_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1150859.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4729..5025
                     /locus_tag="SCK06_000836"
     CDS             4729..5025
                     /locus_tag="SCK06_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1150860.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5197..5817
                     /locus_tag="SCK06_000837"
     CDS             5197..5817
                     /locus_tag="SCK06_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG1150861.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5909..7075)
                     /locus_tag="SCK06_000838"
     CDS             complement(5909..7075)
                     /locus_tag="SCK06_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1150862.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7087..7440)
                     /locus_tag="SCK06_000839"
     CDS             complement(7087..7440)
                     /locus_tag="SCK06_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1150863.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7568..8590
                     /locus_tag="SCK06_000840"
     CDS             7568..8590
                     /locus_tag="SCK06_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG1150864.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8609..8770
                     /locus_tag="SCK06_000841"
     CDS             8609..8770
                     /locus_tag="SCK06_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150865.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9029..9463)
                     /locus_tag="SCK06_000842"
     CDS             complement(9029..9463)
                     /locus_tag="SCK06_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150866.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9573..11432)
                     /locus_tag="SCK06_000843"
     CDS             complement(9573..11432)
                     /locus_tag="SCK06_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1150867.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11550..12710)
                     /locus_tag="SCK06_000844"
     CDS             complement(11550..12710)
                     /locus_tag="SCK06_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG1150868.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13017..14441)
                     /gene="araA"
                     /locus_tag="SCK06_000845"
     CDS             complement(13017..14441)
                     /gene="araA"
                     /locus_tag="SCK06_000845"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG1150869.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14457..15155)
                     /locus_tag="SCK06_000846"
     CDS             complement(14457..15155)
                     /locus_tag="SCK06_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1150870.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15112..16773)
                     /locus_tag="SCK06_000847"
     CDS             complement(15112..16773)
                     /locus_tag="SCK06_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG1150871.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16991..18391
                     /locus_tag="SCK06_000848"
     CDS             16991..18391
                     /locus_tag="SCK06_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG1150872.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18643..19722)
                     /locus_tag="SCK06_000849"
     CDS             complement(18643..19722)
                     /locus_tag="SCK06_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1150873.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19808..20767)
                     /locus_tag="SCK06_000850"
     CDS             complement(19808..20767)
                     /locus_tag="SCK06_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1150874.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20779..22290)
                     /locus_tag="SCK06_000851"
     CDS             complement(20779..22290)
                     /locus_tag="SCK06_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1150875.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22312..22876)
                     /locus_tag="SCK06_000852"
                     /pseudo
     CDS             complement(22312..22876)
                     /locus_tag="SCK06_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(22882..23745)
                     /locus_tag="SCK06_000853"
     CDS             complement(22882..23745)
                     /locus_tag="SCK06_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1150876.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23759..24655)
                     /locus_tag="SCK06_000854"
     CDS             complement(23759..24655)
                     /locus_tag="SCK06_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1150877.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24830..26119)
                     /locus_tag="SCK06_000855"
     CDS             complement(24830..26119)
                     /locus_tag="SCK06_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1150878.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26091..27608)
                     /locus_tag="SCK06_000856"
     CDS             complement(26091..27608)
                     /locus_tag="SCK06_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1150879.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27806..28696
                     /locus_tag="SCK06_000857"
     CDS             27806..28696
                     /locus_tag="SCK06_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG1150880.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28769..29593)
                     /gene="nadE"
                     /locus_tag="SCK06_000858"
     CDS             complement(28769..29593)
                     /gene="nadE"
                     /locus_tag="SCK06_000858"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG1150881.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29605..31077)
                     /locus_tag="SCK06_000859"
     CDS             complement(29605..31077)
                     /locus_tag="SCK06_000859"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG1150882.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31714..32076)
                     /locus_tag="SCK06_000860"
     CDS             complement(31714..32076)
                     /locus_tag="SCK06_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG1150883.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32299..33051
                     /locus_tag="SCK06_000861"
     CDS             32299..33051
                     /locus_tag="SCK06_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1150884.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33191..34114)
                     /locus_tag="SCK06_000862"
     CDS             complement(33191..34114)
                     /locus_tag="SCK06_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1150885.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34129..34401)
                     /locus_tag="SCK06_000863"
     CDS             complement(34129..34401)
                     /locus_tag="SCK06_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150886.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10202 a         7200 c         5954 g        11216 t
ORIGIN      
        1 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
       61 ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa tagcttctca
      121 tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg tttattcgga
      181 aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa atttgtacta
      241 ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata gaatccgtct
      301 ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt catcacaata
      361 atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact tgtccggttt
      421 tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc tacactagat
      481 gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc aattgtgatt
      541 agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt gttatatcct
      601 tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc aatgatttgt
      661 tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc tgtaaacagc
      721 caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga aagatcacgg
      781 gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg ttcaagaagg
      841 ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat ctcaccaggt
      901 ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc gaattgaaca
      961 ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga tttttcattg
     1021 gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac tgttgattgg
     1081 atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt tgtatattgc
     1141 aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac tccgcctagt
     1201 acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat gatgattgcc
     1261 gaaataaatt gagctttcca accagcggca tacagattct gattttcttt ttcaaatact
     1321 tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc atatgtttct
     1381 tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa cacttgtgat
     1441 ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa cgcgactaac
     1501 gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt gactgcacta
     1561 gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat atcattgatt
     1621 gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg tacacgattc
     1681 attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac acgtgtcata
     1741 ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat tactgtcgca
     1801 atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg gccttgttgc
     1861 atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt ccccaagacc
     1921 tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac agcgatgatc
     1981 gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca gaaattttta
     2041 ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg accgcctgct
     2101 ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct cttagttgtg
     2161 aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt gttcctttgc
     2221 caaccagttt tccttcatct agcacaagga tcatatctgc atccattact gtacttactc
     2281 gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct tggcgaagat
     2341 tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag atatctgctg
     2401 ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag aagtttcctc
     2461 ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa tctttagctt
     2521 gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta ccatactgca
     2581 tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag cccattaatt
     2641 ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg atcgaaccag
     2701 attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct gaaccagtcc
     2761 cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct tccaatgcaa
     2821 gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt gcgttttttc
     2881 cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt tcatccaaca
     2941 cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa atagcggaaa
     3001 gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc agattaccga
     3061 cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct acgtttgtac
     3121 cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta actttgattg
     3181 ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag cgtgtcttat
     3241 cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg tttaaacggt
     3301 cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt ccgccgatca
     3361 aaataatcat tactgggatg acaactaaga agatttttgt caactgatga tctttttgat
     3421 aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata cgtaaaaaca
     3481 tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc aaagaggccg
     3541 ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag acatccgtac
     3601 gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt gcgatagcag
     3661 ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa atataatcga
     3721 catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc aaatataaat
     3781 ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc aaggagatcc
     3841 gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg tttttcagcc
     3901 aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct tcagatgttg
     3961 aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact aattggatca
     4021 gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc aattcaagca
     4081 actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt tggcgactcg
     4141 tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag ttacgataaa
     4201 aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag gattcgaatc
     4261 ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta gagctgtcac
     4321 gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa tactgataga
     4381 aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct tcttttaagg
     4441 gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt tctttaggca
     4501 aatggaaaaa tgtctgtgtc ggcatatgga tcctcctttc tttcaagatg acacattgtc
     4561 atatgacgct gtgtcattat aacacctcct acctgttttg caagacattt gcatagtttt
     4621 tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat ttgaattaat
     4681 gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat ggcccaatca
     4741 attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt tttagataca
     4801 agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat ccccgctgct
     4861 atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat tctaagctat
     4921 acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt tcgtgctgtt
     4981 tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt gcttgttaga
     5041 ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct gtcttttttt
     5101 agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt tctcatagtt
     5161 ataacaatca tagcaataaa aggagaaggt aagcatatgg catctgtttt agtagtcaaa
     5221 ggtcatcccc taactgcaga agaatcgcgc acagttaagg cattgacgtc ttttttaaca
     5281 agttataaag aaaatcatcc agatgatgaa gtgactgttt tggaacttta tcgcgatgat
     5341 attccggaaa tcgatgaaga attattatca ggttgggaag cattacgtgc tggtgcagaa
     5401 ttcacttcct tatcagaaag tcaacaacaa aaaattgctc gtttcaatga attgactgat
     5461 cagttccttg ctgctgataa aatcgttatc gcaaacgcct tatggaatct aaatatccca
     5521 acaaaattaa aagcttggtt cgataccgtc aatgtagctg gaaaaacgtt ccgctataca
     5581 gaaaacgggc cagaaggatt agtgacaggt aaaaaagcac tgcacatcca atctaatggc
     5641 ggtgtataca acggacaaga ctttgcctct caatatgtaa aaggtatttt aaactttgta
     5701 ggcatcgatc aagtagatca gctattcatc gaaggaatcg attatgaccc tgaccgtgca
     5761 gatgagttga tgcaaaatgc attagataaa gctgctgctt taggtaaatc attttaaaaa
     5821 ttgagataaa tgcatacaaa agagacagag gaaaaaatct tcaaagcagc atttgtacct
     5881 tggatatttt cccatctgtc tctttttgtt attctttctg ctttttcaac ggattttctt
     5941 cataaagaga agagattgtt gtgtaccatt caaaaacatg agtaatcact actttagctg
     6001 ctgcataaac aggaatcccc aagatcacac cgatgattcc gaacaatttc ccagaagtca
     6061 gcagaactaa taaaatcgtt actggatgga ttgctaactg attccctagt actaatggag
     6121 agatcaagcg tccttctatt gtctgctcta ctgcaaaaac gatcagtact ttgatgatca
     6181 tgaatggacc tgctacaatg gctaaaaaga tagccggaat catcgctaaa aatgatccta
     6241 agtaagggat caggttcaaa aaacccgcaa taatccccaa cgtgacagca tactctaaac
     6301 caatgaccgc aaaaccaatc ataaacatga ttgccacagc aaaagccaca gttagttgtc
     6361 ctcttatata agaagaaact tgactattca tttcactcag cacattcaac gtcggttttt
     6421 gcatgcgtgt cggtaaaaat ttgacaaaat aaggtgctaa ttgtttaccg tctttcaata
     6481 gataaaataa gataaacggc atcgtcagca gagccactaa aatagtcgct accgcgccga
     6541 caaaacttcc aagactctga actgtactct tagatatatc ttggatcata tcgcctgccg
     6601 aattcatgaa cttatcacct gctgtttcca actgttcgcg aaattggttg aacagcgggt
     6661 cccgcaaaat ctcggttaat ttattatcaa tcgtatctac atattgaggg aagttactga
     6721 tgaagctcac tgtttgttcc tgaatcttag ggatgatcac tacgctcccc catgcgatta
     6781 gtgcaacaac caaaacaaac agcccaatga tactgtaaag ccttggtact tttcgctttt
     6841 caaagtaatc aactaccgga ttcatcaagt aatacaagat tcctgctaaa atgataggca
     6901 gacctacgat tgctaaaaat tgccagattg gtgcaaacaa atatgaaact tttgtaaata
     6961 aaaagacgat cagcaatatt aacaatacaa caagtaaggc tgtgactact tgattattta
     7021 aaaaccagcg ccaaaaccac gataccggtc tgttcttctt attttttcct tccataattc
     7081 ctcctgttag ttataaaaaa tttggcttcc caaaccaata gcaggcagtc tatgcggatg
     7141 aagatagatt ccatttgcaa tttgtccttc ttctggacaa gcatcaaaaa tcaatgatac
     7201 gtgagctact gtctctaaat tcccattggc catcggaccg acataatcga tcgtatatac
     7261 ctgatcatca aaagcaattg ttccgcctgc tttcaaagaa aaaggttcgt cttttgatac
     7321 ttcttgaatc accgaatatt ttcgtaaggc agctgttgct gttttatcga acaaaatcag
     7381 catgggttct tccttgttta ttgcttctgt cccaatttct gtaatctttg cctgaatcat
     7441 tgcactctct cctttaattg ctttcaaaaa cagtataaca cattctcatt ctttagccgt
     7501 gtggaataat tatgtgatag aataaaaacg aaaactattt cattacaatt aggaggaatc
     7561 agctcatatg atcgaaaaga taattttagg aacctatacg cgtcgcgaaa gtaaaggaat
     7621 ctatacgatc gaccttgata cagaaaaaga agaattagcc aacctttcat tagctgttga
     7681 agaaaatagc cctacctatg tagcaaaaag taaagctggc aatctttaca cagttactag
     7741 tgtggatgga ttaggtggtg ccggtgcgta cgataaggat tttcattttt tgaatgcggt
     7801 aacagagtct ggcgctcctt tgtgttatgt agcggttgat gaagcacgtc aattactata
     7861 cggggcgaac tatcataaag gtgaagtaaa tgtctaccat atattaggag atggcgaact
     7921 gaaagcagca gattcaatat tccatcaaga agcgacaggt cctcataaaa accaagatca
     7981 tgcgcatgtc cactatacgg atctgactcc tgaccaacga ttagtcgtat gtgatcttgg
     8041 tacagatcgt gtgtatactt atgatgtgtc cgaaaacggc aaattgaatc ttgtaactac
     8101 cttcactgca gagccaggaa caggccctcg ccatcttgtc tttcatccaa atggacaatt
     8161 cgcttattta tttggtgaat tagacagcac agtccgcgtg ctttcttata ataaagaaac
     8221 aggtgctttt gaagaattac agaaaatatc gaccttgcca gaagaattcg acggtgaaaa
     8281 tggaggggca gctatccgca tttcaaatga tggcaagttc ctttatgctt ctaaccgagg
     8341 acataactcg attgctgtat tcgcagtttt tgaagatggt tcccacattc aaaacaagca
     8401 aatcatctca acagaagggg attttccccg cgatttcgca ctagatccaa gcaataattt
     8461 tgtcgtgtgt gccaatcaga acaccgataa tctgacatta tatcgccgtc atcctgaaac
     8521 aggattactg gaattactga aaaaagatgt ctatgtacca gaatgtgtct gtgtttattt
     8581 tgatcgttag taagaaaagg agaatgaaat gaatcagcaa gacgtattag acagactacg
     8641 ggaagaacta aatattcctt tctttgatgg gaaaatcgaa gataaagagt atagcgaaga
     8701 ggaatatcag aaactcaaag ccgatttaca gaactacttt gaacaatatg tacagaatgt
     8761 agaaaactaa tcagctttcc atactatctg aaaagaacat caggacaaga cttttccgtc
     8821 tatcctgatg ttcttttcta caaataaaaa accgccgaga tggttagact cgacggaaaa
     8881 ggagtattta ctcgattttg agtaagtaca aggttgttgt tggttatagg tatgtcatta
     8941 tcataaagga ttttgatcac tctatcataa aggtaatatt acatttatat tagataactc
     9001 tagtttttat tgttatcaaa gatttctttt attttttcaa caacaaatta gtcatttttt
     9061 ggatggcagc tgtcaattgg tctgtcgctt ggtcttgttt tgcttcagat gtattgaata
     9121 gatcttcatc tacccctgat tcaagatcga tactttggat catttcttca cagcctgcta
     9181 catatgaaag ataagctttt tcaaaatttt tatgaatgcc cattgctttt gctggtggac
     9241 gcaaagtact gactttctta agcattgccc gatatttttt tgttccttct tcaaataaca
     9301 ggatgatctc ttcaagagtc gatttgtcca gttcacttgt ttttccttca tcaattgcgt
     9361 ttctgatcac ttcatatttc ggatgcattc cttctccgat tgcttctgtt tccttgacga
     9421 tttgatcgat ggactgcaca taatagccaa tattcggtaa catgttcgtt ttcctacttt
     9481 ctccattgtt gttattattt attctacttg tttttatcct caaatgacaa gtacttgaaa
     9541 aaatttaatg gtttctatac agctcgccat tcttattcgt gctcaaagaa accatctgct
     9601 aaactatatt gtttccctgc gacaatgtca tattggatca attgatattc gtcgtaaatc
     9661 tcttgttgcg agcgattgaa ttgcgcttct ttataccact gtccgttttg atagtacagt
     9721 tctcgttcaa acgccggaag ttcctgttct aatgataaaa gcagctgata aaagccattt
     9781 gttgtctgat tcgtctgttc cattagattc gctgcaaaat aaaacggact ggtgattgca
     9841 tcgtgagtcg tttgcttttc taattctcct ttactgtcga acataagaaa ttgtgtttca
     9901 tgcaatgttt gtttgtcatt ttttgcttgg atcgtatctg aatagatccc tggaagatga
     9961 tccccccaaa aaacaactaa agtccttcta gaaagattct tcaattcttc cgtgaactgc
    10021 ttcagtgccg tgctgctata ggaaatatct tgcagataat tctctaagtc aagtgtccca
    10081 cttccatctg aagtttttgc aggatagtct agattatcat actttccatc gtagggcata
    10141 tgtgtctgca tcgtgactaa atgaatgaac tgaggtgttt tttcttcctt gagaagtgtc
    10201 attacttctt tgtaagctga ctcatcagaa atatatggat tattctctat cgtatccgta
    10261 taggtcatcg tacgctcgct gatgaactgg tcaaatccta atgtctgata aacatcctct
    10321 cttttataca tcgaagtatt gtatggatgg atcgctgtcg tctggtatga ttgtgcgttt
    10381 aacgctgaaa cgatagaagg aagctgatcc atttttggta caagcatcgt gtaaggggtg
    10441 gtgagttgcg cattaaataa cgccatagaa aaaccagtca atgcttcaaa ttcgatattt
    10501 gctgttccac cgccatagtt ttgagaaagc atattgccac tatatgtttg atcagctact
    10561 tcataataat ctgccaatgg ctcaccagat acttctacgc cgtttaatcg actaggatct
    10621 gagaaacttt cactcatcac gaacacgatg ttcggcgact cttcctctac agttttattt
    10681 ttttcatcgg ctagcttctg gtatttttca gtgatttctt tgatcttttc tttcgaatat
    10741 ccttcaggtt catccattgg ttctaccttc agattgtaca aaaacccacc gataaaacca
    10801 gtgttatagt agttcatttt ctggctgtaa ggtatccata gtgccgtttt attatatgct
    10861 tttcgcaaca aattatcggg attgttgaaa ttactgatat accctagcag tccaattgcc
    10921 acaagcagtg tcgttaatcg gatcaattgg aatcgccttc cttttttcag gcttcgaaac
    10981 atgtaccagc aaaacaatcc tatcacggtt cctgcggcag ctaagatcac tgtaaacagc
    11041 atagtgccgg tcatatcttt cagcaatccg atttcagtga tcatcttcaa atcgtctgga
    11101 taaatcggtt cttgccgata actcattttc atataatttg caaaacctaa tacaccgatg
    11161 gctacgacat acgtaaggga acctaaaggg acagatcctg ctagtgcaat cagaaaaata
    11221 agaataatca gcaagacgag acaggctaag aagaattttt ccgtatgcca agaaaaagca
    11281 aatttcaacg caaggtctac ggatagatca ttttgacacc actgcaaaaa taaattacta
    11341 aaaacaacaa gaaaaaagat actgatgatt gctgctgttc cttttaaaat ctttttctgt
    11401 ttggtcgaaa gtctgcttat atatcttttc aaatgcttgc tccttcattt ctatcaataa
    11461 aagaatgttg cttctcttat tttagttgta cgaaattcga aagtaaagaa cggaaagaag
    11521 aattaatgaa atccaaatat tctctttctt tatggccttt ctggtttctt taataagcgg
    11581 acctttcgta ctccagaaat tttttctagc tgttgtttca cttgattgag ctgttcaaaa
    11641 tcagtactgt ctatgtcaat caatatgtag acatgctcat tcttatggtt acgcgatata
    11701 tctgcgatcg ctagctcgtt ctcattcagc agagaaaata tctctgctaa tatattggtt
    11761 ttcttttgat aaaatatagt gaatcgaaac ggcgcttgga aaaacattcg tgcggatgga
    11821 taattgaccg cttctctaac cgtaccgaac aaaagataat cttttaaggc ttcaactgcc
    11881 aaacgtccgc tgtctgccaa agcttcctga gtcgatcccc ctagatgagg caacataagt
    11941 atacggtcat tttctaagaa ctcttcttct ggaaaatcag tcaagtattt agccagatgt
    12001 tgtttttcca atgcttgcat aacagcatat ttatcaacaa tttcacttct tcctacattg
    12061 ataagtacag catctttttt cattttttct atattcgcct gatcgatcaa tccttttgta
    12121 tcttctgtca acggcagcat gactacgatg taatccgagg tagaaagaac tgtttctaaa
    12181 tctgcttgcc gaacataatc caactgggca tcgcggatag aataacccaa gacatccatc
    12241 cctaagctat agcaacttag cgctactttt gtgcctatcg ctccaagacc aagaagaccg
    12301 atggttttgc cttgcaattc tcgtcccaca taagcacttc ttttgttttc tgcctgttct
    12361 aaaatattag gacctgttag tgtttgtacc atacgacttg cttcgattat cggacgggaa
    12421 gataagagca aacagcacaa aacaagctct tttactgcat tagcatttac acctggtgta
    12481 ttcatgacga tcgtcccgtt ttcagaagct ttttcaacgt tgatcgtgtt caccccaaca
    12541 ccagctcttg aaattgctag caaatctggt gtaagccatt catctttcac tttcgtacta
    12601 cgaataacta ttccttcggc tggcaaatca tctattattt tgaaatattc cccttgtaac
    12661 ttttctatct cttgttctgg aatagcatca atcaaatgaa cgtgatacat aaaaccccct
    12721 ccttactaac aagtatctcg aaatttctgc aattagacaa ctgtttacca tttgacttcc
    12781 tagaagcaaa aagagcagtt agagcagagt aggctgagtg ataaaaataa ggctatgaca
    12841 agttactgcc ataagcctta ttttcgagag caaaatattg agggtgagat atcttgataa
    12901 atggtgttcc gttagctgac attgataata agactaaatt tcaaaaaagt tgaaaagcaa
    12961 atttcgtcaa tctcttctta tttcttaaaa ctaagtggct ttttcacaac ctctttttat
    13021 ctaataacca ctgtttccaa ctcaactaat tttgcccacg tttcaatttg atctgttgtt
    13081 agatttagcg agacaacggt atgatgaccg ccacctgcct gaatccatga atggacacct
    13141 tcatggaaat tcggtttagt tttccataac acacgtgcaa caggtaaatt aggtgcttca
    13201 gtcgttggtt caaaggcttc tacttcattg atcagcagtt tataatgcgt accgaaatct
    13261 gccatcgata cgactacccc ttctcctgct ttcccatcaa atacaagacg cgccggatct
    13321 tctctatctc ccattgataa aggagaaaca acgattcttg gtttcgtcgc agcaagcgtc
    13381 ggatctactt ccatcatatg ggattccaag attgcttctt ggccactagc tagctcgtaa
    13441 gtataatctt ccatgaatcc tgtatctttc cctcgagcca tgattttcag caaccgatca
    13501 atcgctgcag ttttccagtc gccttcccca gcaaaaccgt atccttctgc catcaaacgt
    13561 tgtacagcta gtccaggtag ttgtttcatt ccatacaggt cttcaaaatt agtcgtaaaa
    13621 gcgttatagc ctccttcttc taagaaacgg cgaataccga tttcctgctg agcttgtact
    13681 tttacatgtt cctcccatgt cttttcttca taatcaccat agtcgaaggt atacaattcc
    13741 ttgtactctt caaataattg atcgacttct tcgtctgata cgcgatcgat tacttgtaca
    13801 agatctccga taccaaaata atcaacagtc caaccaaatt gaatctgtgc ttccactttg
    13861 tctccttctg tgacacccac gtttctcatg ttgtcaccaa aacgagccac ttttattcca
    13921 aaactttcat tataggctac tgcaacatcc atccaatcag caatctcttt ttggatctca
    13981 ggtcgtttcc agtagccaac gacgatttta ttttgtttat ttaatcgagc attgatgaac
    14041 ccatattcgc ggtcaccatg agcggattga tttaaattca tgaaatccat atcgatcgtt
    14101 ttccaaggaa tgctttcgtt atattgcgta gctaaatgaa gcaatggttt ttgtagaagc
    14161 tttgtcccgc ggatccacat ttttgctggt gagaaagtat gcatccaagt gatcacacct
    14221 gctacttctt ctcgataatt gacctctttc atgatttttg tgatcgtatc agcagtaact
    14281 gctaattctt gaaggcggat tggataaggc aattggccat tttcattcag ttcatcgacc
    14341 atttcttgtg catgtttctt tacttctcgc agtgcttctt ccccatataa atgctgtgat
    14401 cctacaacaa accaaaattc tttttctcca atattcaaca taattagcca ctccttttat
    14461 ttgttttgtc cgtaatacgc attttttccg tgttttcgta aataatgttt atctaaaatc
    14521 cgttgaggca gctcttctga aaatgaattg agctgtctgg taaataaatt catcttgcat
    14581 acttcatcta gtacgactgc gttcattacc gcactttgcg catctttccc ccaagtaaat
    14641 ggaccgtgtc cgtgtaaaag tacccctgga attgctaagg gatcaatttt gcgttctttg
    14701 aatgtttcta tgataacttt tcctgtttca tattcgtacc cgctgtcgat ttcctgttgt
    14761 gtcaaaaatc gagcacaagg taccgatcca taaaaagtat ctgcatgcgt cgtccccatc
    14821 gcaggaacat ccaatcctgc ttgtgcccag atcgtcgccc atgtcgagtg agtatggacg
    14881 attcctccga tttgaggaaa tgaacgatat aaaacagcat gagtaggcgt atccgaagag
    14941 ggattgagtt taccttcaat gatatgattg tccaagtcca caacgaccat gtcgtctgct
    15001 gtcatctctt cataactgac tccgctaggt ttgataacga aatatcgtgt ttcaggatca
    15061 aaagcgctca catttcccca tgtatatttg acgagtccgt gtttaggcaa gtcaagattt
    15121 gcttggtata cttcttcttt cagttgttcc agcattttct atcactcctt tttcttgcaa
    15181 ttgatgaatc gctgtttttt caatgtcgat cccctcacgg taacgtctca taaatatctg
    15241 atagcctgct atttcttctg ctgaaggtgt gatggtgatt ccttggtctt gtccaaacac
    15301 gtcttctgcc aagaagtttt ctaaagactt cccatgtcga tccatcatat aggctgctaa
    15361 taacgcgatt ccccacgctc caccttctcc ggctgtgtcc atcactgtaa ctggtgcctc
    15421 catagcagat gctagaatcc gctgtccaac ttctggtgtt ttaaaaatcc ccccatgccc
    15481 cactaaacga tcaatttgga cattctcaga tttcaatata tccattccga ttctcattgc
    15541 accaaaagca ctggctaaat ggatccgcat gaaattcgct aaatcaaaac ggctacctgg
    15601 tgtacgtaca aataatggcc ttccttcatt tactcctgta atgttttcac cggaaaaata
    15661 accataagaa agtaatcccc cgcaatccgt atcgccttct agcgctttgt tgaataatac
    15721 cgtgaataat ttatctctgc ttatctccat acccaaagaa ttggtaaatt cttcaaaaat
    15781 actgacccaa gcattgatat ccgaagagca gttattcgta tgaaccatgc ctactagttc
    15841 tcctgaaggt gtagtgacca tgtcgatttc cggatggaca ttttctagtt ctttttctaa
    15901 tacgatcata gcgaaggcgg aagtaccagc cgaaatgttc cctgtccgtt tggctacact
    15961 gttcgtagcg accataccgg ttcctgcatc cccttctggc ggacaaaccg ggattcctgg
    16021 tctcaaattt cccgtagggt cgatcagata agcccctgat tcgcttaact tgccagcttc
    16081 ttctcctgct actagaactt taggcaaaat gtgatccaag tcaatcgcta cgtgcgcttt
    16141 ttcgattaac tgaccaaaaa tatttttcat ttcttgatgg taggttttcg ttgaaggatc
    16201 gataggaaac atgcctgatg catctcctac gcctagtact ttctcaccag taagctgcca
    16261 atggacataa cctgcaagcg ttgtcaaaaa attaatttct ttcacatgtt cttctttatt
    16321 caaaatagat tgatacaaat gagcgatact ccatctctgc ggaatattgt attgaaattt
    16381 ttccgttaat attcgttctg cttcttcagt gattgtattt cgccaagtac gaaatggtac
    16441 tagtaactgt tccttgctat caaatgctag ataaccgtgc atcattgcac tgaacccaat
    16501 cgacccgatt gtcgtaagat tctcgccata gatttcgaat acttctgctg ccatttttcg
    16561 gtaactgacg cgcagtcctt tccaaatatc ttctaatgaa taggtccaga taccattttc
    16621 tagtttgttt tcccactcat aactaccagc ggcaatagga gtaaaagatg aatcaatcaa
    16681 tacagctttg atcctagttg atccaaactc aatccctaaa gatgtacgac catctatgac
    16741 atctaacata ctttgcgcat ttacttcctt cataatgatt taactccctc cttctcgtca
    16801 aacctgatca aaccgtcttt ttagtagcgc ttacaatgat tattgtatac ttttgtacgt
    16861 acatgtcaag cggttattta caagaataca ataaaaaaca cttgttttgt acgtacataa
    16921 gttataaaat gaaattgctt acaaaatctg atcaaccgcc ttctcgaaat tctagaagga
    16981 gagaatactt atgaaaaaag aaaaaaaagt ttcctcatcg tttatttatt tttttggcgc
    17041 atttggcggc attctatttg gatatgatat tggtgttatg accggcgcct tacctttttt
    17101 acagcatgat tggggtttgg caggtaaagc ttctttgata ggatggatca cgtcttcggt
    17161 catgttaggt gcgatccttg gcggctcttt atctggtctt ctttctgata agcttggacg
    17221 acgaaaaatg attcttcttt ctgcacttat ttttatggct ggttctgtcc tttctgcatc
    17281 tgcccctcac aatggttctt attttctgat tgcagcaagg attcttctag gtttagctgt
    17341 tggcgctgca tctgccctag ttcctgctta tatgtctgaa atggctcctg ctagactaag
    17401 gggaagattg tcagggatca atcaagtgat gattgcttct ggcatgctcc tttcctatgt
    17461 tgcagattat ttgctaaaag tgttgcctga aacgatggct tggcgcgtga tgttagggct
    17521 cgctgctgtc cccgcgctga ttttattttt cggtgtttta gctctaccag agtcaccgcg
    17581 ttttcttatg caatctggcc gcttggaaga ggcaaaaaga gtattgaact atattcggac
    17641 accaaaagaa gctgaacagg aattcgaaca aatccagctg aatgtcaaac aagaaaagac
    17701 aaccggaact tcttggcata ctttgttttt agaaaaatat cgttccttag tcattgccgg
    17761 tattggtgta gcagtattcc agcaatttca aggagctaat gctatttttt actatattcc
    17821 gctgatcgta gaaaaagcga ctgggcatgc agcgagcgat gcattgatgt ggccgatcat
    17881 ccaaggaatc attttagttg ctggttcttt gctctttttg gttattgcag acaagttcaa
    17941 tcggcgaact ttattgaaaa taggcggtag cgtgatggga ctttcattca tcctaccagc
    18001 tgttttagga actgtactgg atgctcatac aaattcgttg ttgattctct tgtttttatg
    18061 tatttatgtc gcattttatt cttgcacatg ggctccactg acatgggtaa tcataggcga
    18121 aatattccca ctagctgtcc gcgggcgagc atctggccta gcctcttctt ttaattggat
    18181 tggttctttc ctagttggct tgctttttcc tgtgatgact gcttctatgt ctcaagaaat
    18241 cgttttcggc atttttggga tcatctgctt tttaggggtt ttatttattc aagagatcgt
    18301 accagaaact cgtgggaaat ctttagagga aatcgaacaa agtgcatcta aaaaaactta
    18361 tcctaaacgt atttctatcc atcattctta aagaggttgt gacagaagtg gtcagcttca
    18421 agaaataaga aggaaccgct tagtttccag tcacaaggaa caatttcaaa aaacagcttc
    18481 tcatgtttga tgaatattgt gacttgtgac cagttggtag gagaacaccg tttagtggag
    18541 aggtggtgaa agaaacgttc agccttgagc gatgacaaaa gctctcacaa acattgcgac
    18601 gagtaaccgt aggagcaata agaggcggta aaaacccgtc tcttattttt cgttttcttt
    18661 cactgtttcc cgttcaacta gtacaggttc atagagaata tttggctgtg cctgtccttt
    18721 ttcaatcgtt gtgacgatcc accgtgcggc atcttgtccc aacttttctt ttggatgcgt
    18781 caatgttgtc aattttttat tccctaaatg gcttaaagaa gaatcgtctg ttcctacgat
    18841 cgataggtca tctggaatat gatagccatt ttctgtcaac ttatctagca ttaaactagc
    18901 tatttgatcg ttatagcaaa tgatgccatc taaagcttgt tcttctttca accgttgtac
    18961 tgttttttca gcgattgttt ctcgtgtttc cgtggtgaaa gtaatgatat cttccgtcaa
    19021 aaaacgcaca ttatactgtt cacacgcgct gataaaacct ttcatcctgt attttccttg
    19081 aagatcgtcg atttttgtga tgaacaacag acgctgatgt ccttttttta tcaaatattc
    19141 tgctgccata aaaccgactt tcacatcatc taaacaaata taagaggcat ttaactcttc
    19201 ataagcagca ttgatcatca ccatcggaat gccttgttct ctcaaagcaa cataaagtgc
    19261 taagtttggg ttatactgat tacttttcgt aggctcaacg atcaaaccat ctaccccata
    19321 agcaatcatt ttcttcaaac actctttctc caattcgtga tcgttattcg tactggctaa
    19381 aagcaatgaa tacccagatt ctcgtaattc attttcgatt cctcggataa tcgacggaaa
    19441 aatataatca gagagatatg tggtgatgac accgatcgtt tttgttctgt tcaatggtgt
    19501 tttttctttc ttatagctgt catctacata tgtgcccgac cctttttctt tacgcagata
    19561 gccctcattt accaacacag cgattgcttg ccgaatcgta tgtctgctga cgtcatatat
    19621 ttcttgcagc ttgaattctg agggaatggt tgtatttact tcgtattcct cacgaaggat
    19681 cttttgtttt atctcttctg caatatcttg atattttgcc atttgccttg ccccttttta
    19741 tttgtacgta catatatcat aacgagtctg caaggtttat tcaagcaaaa atcaaggttt
    19801 cttgtgctca taaggcatac ggaacaggtt caccaaattc tggaaatcct tgctcattcc
    19861 acgtaaagca ttgcgcgttc gcgtgccgat taggattgtc taacggatcc cctttttggt
    19921 tcttctctgg tcgagcatga tagatcaaaa tatcaaagca cccgtcttct gaaaccgtaa
    19981 acgagttgtg accaggacca aattgcttat ttttttcaga agaaacgaat actggctccg
    20041 ataatttatg ccatgattct tttaataaaa gattcttgtg ctcatcagcc cacagcaggc
    20101 ccatggcata gttttcatct gttgcactac cagaatacgt aatgaatatt ttcccattgc
    20161 gagtaaggac tgctggacct tcatttactg aaaaaccccg tttttcccat tcatactcag
    20221 gtatagaaag gaggatctgc ttccccttta acgtccaagg attatccatt tcggatagat
    20281 aaagattaga attcccagta atttcaggat cttgttgggc ccacacataa tatagctttc
    20341 cttgagaaga gaaagttgtt ccgtctaaac tgaaagtttc ccttttcgtt tttatttgtc
    20401 ccttttctat ccaatctgta ttcattggat ctatatgctg attttcaagt acgaacatgc
    20461 ggtgatgatg agattcatca cgtatagttt tatgattgct tgcagcaaag taaatatacc
    20521 attttccatt gatataatgg acttcaggtg cccagatcaa ttcactcatc ggtccttctt
    20581 catgagcctg ccatacaatc aggcttgcag catgttctaa ttcattcagt gactttgccc
    20641 ttcttaattc aatcacttgg tagcctggaa ccgatcctgt aaaataataa taaccatctg
    20701 tatgtttata tacccaagga tctgcccgtt gccgaacgat aggattatta taaagtgtct
    20761 tcatcataac aaatttcctc acattttctt tattctaatt acattccaag agtatttttc
    20821 tatccctaca tttaattcgt catgtttttc tattgaaaaa tagtttattt caggttttat
    20881 tttttctggt tcttcacgtg tatttttgtc caacagatcg ctactggtta atgtgtagtg
    20941 ctctccttga tctagcaaat tgaaatcatg ttcaatcgtt atcttttgtt gttcatttgt
    21001 gcgattgatc aagaaaatag tcagctgatt gccgttatct acgatcacac tgtccacata
    21061 aggaacattt tttatctttt cacaatcata aacagggcaa atgatttgtt gtttcaatac
    21121 ttgaccttta gcatatttcg ataagtgttg catcacataa taagttggat tcctccatgc
    21181 ttgtccttta gcatctgtca gtattaaagg tattgtgttt actaacaatg attgacaact
    21241 aatctttacg cgatctgcgt gtcgtaaaat agccagccct aatgaaccaa ataataatgt
    21301 atcttccata gtaaagtaac accaatcgat gggactaccg acttcaaaat ccctatgtct
    21361 tttttccggt tgggcatgta cattccattc atcaaacgct agatacattg ttttctcact
    21421 tctcaaatat ccttttacat aatcacaagt agcaacgatt tcttcaattt gacgatcgaa
    21481 tcgataagct tttgcaaggt atgtcgctgt atcatcacgc tcatttttag gaggtttagt
    21541 caagtcttct tcatcatact tatcaatata gttatgcaaa gctaaataat ctacattctc
    21601 ataagcttct tctaatgcga tacgatccca ttcagggtac gatggtaaac gtggcgtaga
    21661 gcttccaact aagattgttt caattgaatc atccactaac ttcatgactt ttgaggcttc
    21721 atttgctaac ctaccatatt cataagcact cttagccccc acttgccatg gaccgtctag
    21781 ctcattccct aaacaccata atttaacgcc aaaaggcttt ttttgcccat tttttattct
    21841 taaatcactc cagaaggttc cttttggaaa attacaatat tctactaaat tttttgcttc
    21901 atcaattcct tttgttccta aattcaccgt gaatatcggt ttcgcttgta catgactgat
    21961 ccatttaaaa aattcgtgga gtccaaaaga atttggttct atacctcgcc atgctaaatc
    22021 aatttttttt ggtcgttgtt ctttcctacc tacagtatcc acccaatcat aaccagatgt
    22081 aaagtttcct ccaggatagc gaatgacacc taattctaat tcctttacat aagagaggac
    22141 atcttgtcta aacccgtcac catcagctgt ttcatggccc ggttcataaa tccctgtata
    22201 gacgacactt cccatatgct caacaaatga gccaaacagc cgagaactga tctctcctac
    22261 cgtaaaatcc tctctgattt ttactttcac ttcttcgtcc aacagctttc cctacctttc
    22321 atttcctata gtcaagataa tagatacaag tttccatacg ccaccagcaa agataaaaat
    22381 aaaaggaggg aaaagaaata tgagcagtac aaatcctaat aacaatacgc aacaaagaat
    22441 atttctcaaa aaatcaaaaa atactttcca tgcactttgt tcaaaaattt gtaaaaatgt
    22501 gatatctttg ttttcagaaa tcacatatat ccaggataaa agaaacatgg aataaataag
    22561 taaaactaca tatccactaa ttgaaagtac gattgtctgt tcttctaata attgattaac
    22621 aagaaaataa ataatgatcg ttaacagact aaggaacaag ttcttaccaa aatgttgctt
    22681 tattccttga aagaaaaaat aaatgatatt tcctgatccc tcacctttcc attgcgccat
    22741 tacataaaaa aacagcacat gtagcaggca cgatagtaat aacaggcaaa cacacgatta
    22801 cccagagaat acctaataag atgtaatcag aaaccacaat caactgtcca taaagtttag
    22861 tctcaactat tttcatcttc ctcactcttt cattccagac atagctgttc cttgaacaaa
    22921 atatttctgt gcaaaaatat aaaccactag taatggcagc attgaaataa ccgttccagc
    22981 cattacaggt ccataaaaag aaatgttctg ccctgtaaat aaagctaacc ctgctggtaa
    23041 agttctcatt tttgaactac ttgtcaatat caatggataa agcaagtcat tccaactatt
    23101 catcagcgtg aaaatcccta atgataaaag agtaggtttg ctcaatggca acatgatttt
    23161 caaaaaaata ttaaactcac ttaatccatc aatgcgtgct gcctcttcca aagaatctgg
    23221 taaagtaata aaaaaggaac gcatcatgaa gataccaaat gcagttgtca tacgaggaat
    23281 gatcaaacct gcataagtat caagcatgcc aagcaaattg acttcaataa atagaggaat
    23341 catgaagact tggaatggga tcatcattgt cagcaaaacg aaatagaaca aaactgattt
    23401 tcctttgaaa cgcatacgag caaatgcata ccccgcaaga gaatcaaaga atacagaagt
    23461 gatcactgtc cctccagcaa aaatcactgt atttttgatg taatctaata aagggatcgt
    23521 tgtccaaacc ttaatatagt tatctagggt atagctatct gcaaatattc taggcggaaa
    23581 tgagataatg tctttttcgt gtttaaaaga agaaataatc agccacaaaa aaggaaacgc
    23641 aataaccaac aatataacta gcatactaat cgttcgaatg atttttttac cactaactgc
    23701 ttttcgacta ttcacagtaa ctgtaatcga gctttctggt gccatacagg tttccctcct
    23761 atttcatttg tttttctttc cgtgacatgt acacattagt ggagatagct atagttgcaa
    23821 caatgataaa caaaaccgaa gacagtgctg atgcatagcc caattcataa ggtgctgtaa
    23881 atcctcgact gtaaatatat tgaacagctg tttctgtctt aaattgtggg ccaccagcgg
    23941 ttgcaacata tacttggtca aacacttgca tcgaaccaat cagattcgtt aaaatacaaa
    24001 aacctaagga gggaacgatc tgaggtagcg taatgttaaa aaactgctgt cgatttgttg
    24061 caccatccat ttgagcagat tcatacaaag agggagagat tccctgaata gcagtcaaga
    24121 gaatcgtcat agtaacacca aaattcttcc aaacagtcat gatggctacc gtcggcatag
    24181 caagggttgg atcgcgaaaa aattgaggga tcggcaagcc gacttgtgaa aacaagtaag
    24241 gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat aatcgcaatt gacgtcaatg
    24301 aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg attgaatctg gtatttttag
    24361 ataatgctac tgctgctaat aaaccaacga taatttgtgt tggcgtttcg agtacggtga
    24421 aatataaaga atgaatcaac gaattaactg ctcgttcatc actaaatata cgtgtgaaat
    24481 tttcaaaact tacaaaattt cttgatgtaa agaaaatatt caaatctgta aaactaatag
    24541 caaatgttcc taaaagcggt atgacagtaa ataataataa aactaaaaaa gatggagtta
    24601 aaaacaaata cgcagcacga tcaggacgat tccaatattt ggtgaatccg ttcatatagt
    24661 ccacctcatc tttcattttg ataaaatata caatcttggt ttaaacgaat ctgaataaga
    24721 tatccgaaca atcgctgtga tttttgcaat aacacctatc tgcaaatact aggtcgatca
    24781 ttcagatatc tgtatcttgc caacagtcta tcctgtataa agttctgtac tatttttgca
    24841 gtaattgatt gatggcatca tctgcatcgt tcatgagtgt ctcaggatct tctcctgcta
    24901 ataatttttc aatcagtgga ttgatgatat cgttattgat cgcagagatt tgggtaaaac
    24961 ctggtaaata tggctctgca tagtccattt gtttcgataa ttcagataca ataggatcat
    25021 tttttacttc ttcatctttt gctactgaat ccaagtaggc aggaaaacca ttttcgatac
    25081 tccatttttt accaatttca gttgtattcc aatatttcaa aaactcatag acagcttctt
    25141 ttttattatt atctgtactt gccggaatgg catatcctac gccatctaaa atagctgttt
    25201 ttttgtctga atcactccct tgtggtaagg tagtcacatt ataatcaatc tcgtttgctt
    25261 tcaatccgtt atttaaccat ggcccattga tttcaatggc tagttgacca gcattcatta
    25321 aattgtccat ttcggcgcca gaaatcgatt ctggaccagt cttgtctaca tggatcattt
    25381 tttggatcgc ttttaacgtt ttaaggttct caggagacgc aaattcagac ttagtataat
    25441 catcgttcac taatttaccg ccatttgcta acatccagtt atataacggt gcagttccat
    25501 ccttattgaa gacaaaacct gatacatttt tactactatc agttaatttt ggtgccattt
    25561 ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc taatccagct tgtttaaaca
    25621 aatcattatt ccaatacata tacatgcctt gtacctgcat ggggacaaaa tactgtttac
    25681 cttctacttc acctaattca atagccgttt ctttaaaatc agatttatct actccatcat
    25741 attcccagaa atcgtctaat ggttgtaccg ctccgttttc tacatactgt gcaaaatctg
    25801 gataatttaa tgcaataaag tcaggtgctt ttttagaaga aatcgctgta ggaagttttt
    25861 cattcaaatt ggcccaagtc atgatatcca tttcaatttt gatgttttta tcatttgttt
    25921 tattaaattc attaacgatt tcttgcaata tttcaccatc agatgcagta aacccattcc
    25981 aaaaagtcaa agtcacttct ttatcagaat cagattctgc tgaacttccg ccaccgcaag
    26041 ctcctaaaat acctgttgca gctaccaaca aagagccaat taaccatttt ttacctttca
    26101 ttttttgttc ctccttcatc tgataaacga catctgacca cattccaaga tagcggtttc
    26161 aataaacaat tgattttccc atcttcagct tttacatttg ttgaatgact tggttttact
    26221 tgttcacttt taggaagatt agttgctttt gtatcaaatc cactcatttc agaaaaatca
    26281 atcactccct ctaatgataa ttcagacaag tgaacagtta gattcatttc ttcttctcca
    26341 cggttaactg caaatatgac aagttctttg tcatttatta cggctatact ttctaaatac
    26401 ggcacttctt caaaatcttc cgtcgcatag tttgctgaac gaatttgtgg gcttaacacc
    26461 tctccttgtc catacacagc agcttgcata aatggataat agatcgtctg tctccaagct
    26521 tctccgtttg tttctgtcct gattggtgca ataacattta ccaattgagc aagacaagca
    26581 atctttactc tatctgcatg tttcaataaa gtgattaata gacagcctaa tagtaatgca
    26641 tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa tctgccaagg cgctgcttgc
    26701 tcatcttgtt ccgctgaatg ataccaaata ttccattcat caaaggacag gttgatctgc
    26761 ttcttactat gtttcttcgc cttgactgta tcgcacatcg ctgtcacttc agaaatgaac
    26821 cgatccatgt ctaaggaacg tgcaagataa tgagaaagat catttttttg gttgccataa
    26881 tattgatgca gagacaaata atctatttga tcgtacgctt gatcaagaac tgttaactcc
    26941 caatcaccaa atgtaggcat ttgccgattc gaacttccgc ataaaaccac ctcaattgaa
    27001 tcatctacga gtttcatcgc cttagccgtt tcctctgcta accgtccata ttcttccgct
    27061 gttttatgtc caatctgcca aggaccgtcc atttcattcc ctaaacacca tgtcttaatt
    27121 ccaaaagggc tctcttgacc attcgaacgc cttaaatcac tccaataagt tcctttagga
    27181 aaattacaat attcgactaa attacgagct gcatctactc cacgagtacc cagattgaca
    27241 gccatattga tttcggcccc cacatgctga caccagttat aaaattcatg aatcccaact
    27301 tcattcgttt caattgtccg ccaagctaaa tccaaccttc taggtctatt tgctatgggc
    27361 ccaatactgt cttcccaacg aaatcctgac acaaaatttc caccaggata tctaatcatg
    27421 ggaatgttca attcttttac caagtgaatg acgtcttggc gaaatccact tttattagct
    27481 gatggatgat ctggctgata aatcccttca tatactgctc gtcccatatg ttcaataaat
    27541 gaactgtaca accgcggatc aactttgcct acacgcttga cactactaat caacattgtg
    27601 gcttccataa ctgacctcct ttaatttata catgaataat acacctcgtt gaaagcgttg
    27661 tcaataatat aaatgttaaa cttacttatt ttttatcgat tttaattaac caaaatatta
    27721 ataatgatta aaacaaataa tttaatataa cttttattaa caaatatgtt aatatataag
    27781 aaaaaccttt taagaggtga tatatatgca actacaaata aacgaagaat ccttaccggt
    27841 atatgaagca ttagctagta aaacaaggat caagatcatc cagctacttt ccaaaaaaaa
    27901 gatgaatgtc aaagatttgg caaaagaact tggagtgagc agtgctatca cgacgatgca
    27961 cgtgaaaaag ctagaggaag caaacatcat caaaacagaa aaagtaggac aacaaaaaat
    28021 ttcgagcctt cgtgtcgata aaatcgatat cagttttcca gaaaaaatat tcaatgcttt
    28081 tgatacgaaa gaaacatcga tacctattgg tcactataca aattatgcaa ttgaaccaac
    28141 atgcggttta gctacgatcc atgattttat cgggaaagtg gatgaaccac gctattttat
    28201 ggatcctcga aggatggatg cccgcattct ctggtttacc agcggatttg tcgagtatca
    28261 ggcacctaat tttttaaata cagaggatac attggagatg ttggaagttt ctgtggaaat
    28321 ttcttctgaa tttccttttt caaatgacaa ttggccttca gatatcactt tcagcctaaa
    28381 tggtgtggag ctaggaacat ggacgagccc tggagatttt gctgatatcc gcggtaaata
    28441 tacgcctgat tggtatccag ataatcttaa tcaatacggc ttgctgaaga cgatccgaat
    28501 taccaaacac ttgacgaata tgaatggaga acccttgtca aacatcacga tcaacgacat
    28561 ccctaaagag caagacactt ggcatttgcg aatcgaagtg aaagacgatg ccaaacatgt
    28621 ggggggctgt actttatttg gtaaaggatt cggtaattac gatcaggata tcaaattaaa
    28681 agtttactac agctgatatt tttcttttct attttataaa acaaaaacag ccaatcccta
    28741 tgaagggacg gctgtttttg ctttattgtt atttccagaa atcatccaaa acggaaattg
    28801 gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc gattttttct tgtgcttgag
    28861 cgggaacttc ttttccttct agataatcat caatttcatt atatgtgaca cctagtgcga
    28921 cttcatctgc aatcattggt ttcccatctt caagatctgc agtcggaatt tttgtgtata
    28981 atttttccgg tgcattcaat gcttgaagca attgtttccc ttgtcgtttg tccaaacgga
    29041 ataatggcaa aatgtcggct ccaccatcac caaatttcgt gaagaatcca gtaatgtttt
    29101 ctgcagcatg atctgtccct aatacggctc ctgctctttc tccagctacg gcatattgcg
    29161 taatcatacg ttgacgtgct ttgatatttc ctttgttaaa atcgctgatt cccacacctg
    29221 cttcgcttaa tacttgtact tgtgcatcaa cagctggttt gatatttact cgcagactaa
    29281 catcgggctg gataaagtca agagcagctt ttgcatcttc ttcatctgct tgttcaccat
    29341 aaggaagacg aacagcgata aattgatagt tttcatcttt tgtttcttca cgcatttctt
    29401 ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc ttggccgccg ctgataccta
    29461 gtacaaaagt tttaagaaac ggatattttt tcaaatatgc tttcataaag tcaatacttt
    29521 tacgtatttc ttcttttgga tcaattgttg gtttgacacc taattcattg ataatttctg
    29581 tctgcaggct catttattct cctcctatgc ttctgtatga agttctgcta cgcttttttt
    29641 cattcgttcc atgatagcca ttttatggtt ccagcattca gtagacagat cgactgggta
    29701 tttttgcgga ttcaagtctc gtttatactc ttcccatagt gaatctagat tttcttttgt
    29761 atattctttg atttcgttta gtgcaggtaa ttcgtaaact ctttttcctt cgataaagat
    29821 atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt ttattgataa acgtatgcac
    29881 tggatggaac atgaagatag cttcttcttc tcgagggtct tcatcccata atgtcacgta
    29941 gtctccttca gatttcccat caaaatttcg agtgatgcgc cacacttgtt ttttccctgg
    30001 agtagttact ttttctgcat tgcttgataa cttgatcgta tccatcattt ttccttcatc
    30061 atcttcgatc gatactagct tgaatactgc accaagggct ggctgatcat aggctgtgat
    30121 caattttgtc ccaacacccc atacatcaat tttagctttt tgcatcttta aattcaaaat
    30181 cgttgcttca tccaagtcat tcgaagcata gatttttgct tccgtaaatc cggctgcatc
    30241 taattgttcc cgaacacgct tagatatata ggccatatct ccactgtcga tgcgtacgcc
    30301 ttggaagttg attttgtcac ctaattcttt cgccacgcga atcgcacttg gcacacctga
    30361 tttcaacgta tcataagtgt cgactaagaa cacgcagtct ttatgggttt tagcgtaggc
    30421 cataaatgct tcataatcat ttccataaga ttggactaac gaatgagcat gggtcccgct
    30481 agctggtatc ccaaatatct tcccagcacg tacattgctt gttgcatctg ctccgccgat
    30541 atatgccgct ctagttcccc agactgctgc atccagttct tgtgcccgtc tagttccaaa
    30601 ttccaatagt ggatcttctc cgatgacaga tttgatacga gctgcttttg tcgcgatcaa
    30661 cgtttggaaa ttgaccatat tcaacagtgc tgtttctact aattggcagt gcgctaaagg
    30721 cccttccact tggattaatg gttcatttgc aaaaactagt tctccttcac gagcagaacg
    30781 aactgttgct ttaaattcaa aattttgtaa atactccaaa aaaccttcag gatagacttc
    30841 taatccacgc aagtaagcaa tatcggaatc attgaacgtc agattctcta aataattgac
    30901 taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta aaaggcattt ctcgaaagta
    30961 acattcaaaa acagcatgta aatcagctcg acctaattcc cagtatgtct gcatcatatt
    31021 gatttgatat agatccgtat gcagcgtcaa actgtcgtct gggtaagtat tattcatctt
    31081 gtattactcc tcttggcaag ttttcatttc ctacccatta taacaaaaaa aattaatagg
    31141 aaaagaaatc ttccattttt tctgaaaaag cacatgtttt acggatattt cttgctaaaa
    31201 atgaactcca tctgctaaaa ctatcctttc ttattggtat ttaacctaaa cgagaaagaa
    31261 tacttatttt attcaatgta tccgagacac gaagaaatta gcgttttcta tttttcttac
    31321 ttgataaaat aagtagattt tcctactaca atcgtagccg ttaagatacc gatgattgaa
    31381 caaatataac caaatgtctc tacaacataa tcaatggcaa aagtttttga agcaataata
    31441 cgtctttcct tttttgcttc ttatccccaa agaaacggat ataacttatt atgtattgaa
    31501 tttttttgga aaggcgaatt atgacaagat ataccttgcg ttgaaaaaac tcgcggaaaa
    31561 aatatcacac gtttttaaaa agatacaaag taagaaagca gctgtcaata gaatggaaac
    31621 aaaaatggcg agacatcaat cagtctcgcc ttgtttttta ttgtatacat tagatatgga
    31681 gcaggttacc ataaagatga ttattagtcg ttattaaata gaatccttcg tatttttttg
    31741 ttctatcact acaatatctt tatttggacg tttgaattgt attccggcac gttctagctc
    31801 ctgtttcact cctacatgga ctttgacatc ttctcgtgtc atttttaaaa cgtgatgaat
    31861 cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta ttgatcatca cgacttgctt
    31921 tttatcatat gtgtctacca atagcagttg tgttcgatct aacgaaaata atttgtaata
    31981 attaatagcc gtattcccaa aaaagaattc cactacttta ggataaaatg tcttaatatt
    32041 taaattacta ttcacaggtg tttcgattag cttcatctca ttcctctttt ctatatagtc
    32101 gatttagatc caatcttaag ttatgttacc atctttccta gcattaagga atcggttaca
    32161 attgaacctt taaagacaaa aggatttacc ttttatcact taatagtata acataatttc
    32221 gtgagaaaaa aatacaattt tttcttgacc atctcgttac gtaaaggatt atgcttacat
    32281 ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa tgtcagagat atcaggagtc
    32341 agtccgagaa ccttgcgctt ttatgatgag attggactgc taaaaccagc taggatcaat
    32401 tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca catcttattt
    32461 tatcgaacaa tggcattcaa gttggatgat atccaagagg tcttagacaa tccatctttt
    32521 gaccaccaga aagctttaat caagcatcag caaatgttgc tggaaaaacg ggcacaaatc
    32581 gataccctct tgacaactgt ccaacagaca cttgatatgt acgaaggagg aagaaaaatg
    32641 agtgacaaag aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa tgaagaatcc
    32701 tatggacaag agattcgaca aaaatacgga aaaaaaaagg ttacagcatc caatgaaaaa
    32761 tggatgaata tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa gcagatgtta
    32821 tttgatctca ctctttactt aaaagaacca gaagatgaaa gcttagcaga ccgtatcttc
    32881 caagctcata agaagtggtt ctcttacagc tggcctgaat atcagccaaa agcgcataaa
    32941 ggactaggaa tgatgtacga agccgatgaa cggttcactg catattatga cgagcgaagt
    33001 ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta atcttttaca
    33061 tgatccaaca aaaaagtaca cgtaaaatga cctaataaca aaacagcaga cacccaagtt
    33121 agatttttat gctaactttt ggtgtctgct ccattctttc acaagttttt tcatcctact
    33181 ttaaatatat tcatcgttga ataaacgtat aaggtatttt cactttatcc tttttttctg
    33241 taagaaacaa ttgaaatgcc atttctccgc aagcttccag atggtggtcg atggttggga
    33301 aaccaagaag ctcgctgatc agcaaattat cgcgtccaat caccactggt gtcttatctt
    33361 gcagatagct ttgcaagatg cctgctgcaa ccatatccga atttgtaaag acaccatcaa
    33421 ctccaagctc tttaaaaaag aagcctgctt gttttccttt ctctgcatct atacaatccc
    33481 aaaaaacaag tgattcatca aaagatggaa aaagttgatg acatagctgc aaagtgattt
    33541 ttgagttata acttagcctg ccacttcgcc ccaacgtgat gcccaattgc tttatacctt
    33601 gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc tcgatcaata aagacacagc
    33661 ttgcctgttt tcccttgatt tcttcacaaa aaactagagg tccatatttt tgatatgcca
    33721 ataaatcatc tagtgtattg gcacgagacg taatgatcaa tccatcgaaa gctttagctg
    33781 caaattcttc aagatacctt cgctcttttt ctttatgata gcccgtcggt aaaagtgtca
    33841 ctttgtaatc ataatcaaac gctgcttcaa tgatcccact gatcaactga tcaaaatacg
    33901 gatgattggt ataaggcaga atcactccaa tgcttttcgt ttttccataa cttaactgtc
    33961 tggcatttgc attagggata taatcgactt ctttcatgac cgcaagtatt tctttccttt
    34021 tttcctcatc tacatatgga tgtccattga ttacacgtga aatagtagaa actgaataac
    34081 ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc ttcgccacct atacaaaaag
    34141 attttcttgt aaatagtcta ccacagctgg accgcattca aaaaactgat acatttttcc
    34201 ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata tcttttgtca aattttcgat
    34261 ttcattcatt ggtatttctt ttactatatt cctattcaac ccttgaaaac tgtacaacgt
    34321 taatacggat cccttgaact cgacaaacgc ataaaaataa ttgttcgttg tgccatctgt
    34381 tatctctaca taagtattca ttgcaagccc tccttttgat actttgagta tagtgtatga
    34441 aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa gttttattcc tctttcatta
    34501 ttaccaaagt ctcttatttt aaattgaata cattataatg atactgtaaa tatatcttta
    34561 ttattagtga at
//
LOCUS       DARBER010000018        32178 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARBER010000018 DARBER010000000
VERSION     DARBER010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.018"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(407..766)
                     /locus_tag="SCK06_000864"
     CDS             complement(407..766)
                     /locus_tag="SCK06_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG1150887.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="SCK06_000865"
     CDS             complement(796..1137)
                     /locus_tag="SCK06_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150888.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="SCK06_000866"
     CDS             complement(1134..1808)
                     /locus_tag="SCK06_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1150889.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCK06_000867"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCK06_000867"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG1150890.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="SCK06_000868"
     CDS             complement(4322..5512)
                     /locus_tag="SCK06_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1150891.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="SCK06_000869"
     CDS             complement(5533..6060)
                     /locus_tag="SCK06_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG1150892.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="SCK06_000870"
     CDS             complement(6107..8896)
                     /locus_tag="SCK06_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1150893.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="SCK06_000871"
     CDS             complement(9043..9243)
                     /locus_tag="SCK06_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1150894.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="SCK06_000872"
     CDS             complement(9696..10016)
                     /locus_tag="SCK06_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150895.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="SCK06_000873"
     CDS             complement(10519..11637)
                     /locus_tag="SCK06_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150896.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="SCK06_000874"
     CDS             12108..13415
                     /locus_tag="SCK06_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG1150897.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="SCK06_000875"
     CDS             13536..14735
                     /locus_tag="SCK06_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1150898.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="SCK06_000876"
     CDS             complement(14762..16030)
                     /locus_tag="SCK06_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG1150899.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="SCK06_000877"
     CDS             complement(16200..16841)
                     /locus_tag="SCK06_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG1150900.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="SCK06_000878"
     CDS             complement(17250..17753)
                     /locus_tag="SCK06_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1150901.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="SCK06_000879"
     CDS             complement(17876..18640)
                     /locus_tag="SCK06_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG1150902.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="SCK06_000880"
     CDS             18748..19023
                     /locus_tag="SCK06_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG1150903.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="SCK06_000881"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="SCK06_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1150904.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="SCK06_000882"
     CDS             complement(20579..20980)
                     /locus_tag="SCK06_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1150905.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="SCK06_000883"
     CDS             complement(20994..21803)
                     /locus_tag="SCK06_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1150906.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="SCK06_000884"
     CDS             complement(21790..22680)
                     /locus_tag="SCK06_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1150907.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="SCK06_000885"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="SCK06_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="SCK06_000886"
     CDS             complement(23348..24289)
                     /locus_tag="SCK06_000886"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG1150908.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCK06_000887"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCK06_000887"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1150909.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="SCK06_000888"
     CDS             complement(25274..26470)
                     /locus_tag="SCK06_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1150910.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="SCK06_000889"
     CDS             complement(26488..27216)
                     /locus_tag="SCK06_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1150911.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCK06_000890"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCK06_000890"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1150912.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="SCK06_000891"
     CDS             complement(28628..29086)
                     /locus_tag="SCK06_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1150913.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="SCK06_000892"
     CDS             complement(29083..30546)
                     /locus_tag="SCK06_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1150914.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCK06_000893"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCK06_000893"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1150915.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="SCK06_000894"
     CDS             31602..>32178
                     /locus_tag="SCK06_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1150916.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DARBER010000019        30787 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARBER010000019 DARBER010000000
VERSION     DARBER010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.019"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..297)
                     /locus_tag="SCK06_000895"
     CDS             complement(133..297)
                     /locus_tag="SCK06_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150917.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="SCK06_000896"
     CDS             592..1920
                     /locus_tag="SCK06_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG1150918.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="SCK06_000897"
     CDS             2122..2955
                     /locus_tag="SCK06_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG1150919.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="SCK06_000898"
     CDS             2971..3708
                     /locus_tag="SCK06_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG1150920.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="SCK06_000899"
     CDS             complement(3863..4831)
                     /locus_tag="SCK06_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG1150921.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="SCK06_000900"
     CDS             5063..5896
                     /locus_tag="SCK06_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG1150922.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="SCK06_000901"
     CDS             5930..6769
                     /locus_tag="SCK06_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1150923.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="SCK06_000902"
     CDS             6795..7355
                     /locus_tag="SCK06_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1150924.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="SCK06_000903"
     CDS             7554..9275
                     /locus_tag="SCK06_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG1150925.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="SCK06_000904"
     CDS             9440..10582
                     /locus_tag="SCK06_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1150926.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="SCK06_000905"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="SCK06_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG1150927.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12382..13029
                     /locus_tag="SCK06_000906"
     CDS             12382..13029
                     /locus_tag="SCK06_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1150928.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13158..13374
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13422..16067
                     /locus_tag="SCK06_000907"
     CDS             13422..16067
                     /locus_tag="SCK06_000907"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG1150929.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16377..17690
                     /locus_tag="SCK06_000908"
     CDS             16377..17690
                     /locus_tag="SCK06_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG1150930.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17680..18333
                     /locus_tag="SCK06_000909"
     CDS             17680..18333
                     /locus_tag="SCK06_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1150931.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18388..19080
                     /gene="radC"
                     /locus_tag="SCK06_000910"
     CDS             18388..19080
                     /gene="radC"
                     /locus_tag="SCK06_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEG1150932.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19230..19430
                     /locus_tag="SCK06_000911"
     CDS             19230..19430
                     /locus_tag="SCK06_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1150933.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19684..21363)
                     /locus_tag="SCK06_000912"
     CDS             complement(19684..21363)
                     /locus_tag="SCK06_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1150934.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21365..21577)
                     /locus_tag="SCK06_000913"
     CDS             complement(21365..21577)
                     /locus_tag="SCK06_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG1150935.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21970..23700
                     /gene="efrA"
                     /locus_tag="SCK06_000914"
     CDS             21970..23700
                     /gene="efrA"
                     /locus_tag="SCK06_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG1150936.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23697..25457
                     /gene="efrB"
                     /locus_tag="SCK06_000915"
     CDS             23697..25457
                     /gene="efrB"
                     /locus_tag="SCK06_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG1150937.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25552..25749
                     /locus_tag="SCK06_000916"
     CDS             25552..25749
                     /locus_tag="SCK06_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150938.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25902..26387
                     /locus_tag="SCK06_000917"
     CDS             25902..26387
                     /locus_tag="SCK06_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG1150939.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26491..27084)
                     /gene="clpP"
                     /locus_tag="SCK06_000918"
     CDS             complement(26491..27084)
                     /gene="clpP"
                     /locus_tag="SCK06_000918"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG1150940.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27264..27791
                     /locus_tag="SCK06_000919"
     CDS             27264..27791
                     /locus_tag="SCK06_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150941.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27882..28619
                     /locus_tag="SCK06_000920"
     CDS             27882..28619
                     /locus_tag="SCK06_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1150942.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28623..29540
                     /gene="miaA"
                     /locus_tag="SCK06_000921"
     CDS             28623..29540
                     /gene="miaA"
                     /locus_tag="SCK06_000921"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG1150943.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29542..30771
                     /gene="hflX"
                     /locus_tag="SCK06_000922"
     CDS             29542..30771
                     /gene="hflX"
                     /locus_tag="SCK06_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG1150944.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10169 a         5266 c         6568 g         8784 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt gcgaaaaagc
    12181 tgccctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat tttacgtttg
    12241 gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt tttatttgtc tcacaccctt
    12301 ttccttccta cttgtaacct tgctcccgtg ttagaaacat gttacactaa atatgtgaaa
    12361 ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata cactgccaaa agcaactgcg
    12421 aaaagattac ctttatattt acgctacttg aagatgctgg atgactcagg tataagtaga
    12481 atcaaatcaa atgagttcag cgaaatcacc caaattcctt ctgcaacgat ccgcagagac
    12541 ttctcgcaat taggcgagct aggcagaagc ggatatggct atgatgtccc ttttcttatc
    12601 gatgtattca ataatatcct taatactaaa gaagaaaaac gaatcgcatt agtaggatat
    12661 ggaaatcttg gcaaagcatt gaaacacaac aattttagaa gaaatgaaaa tctaaatatc
    12721 gtgtgcgtct ttgataatga tccagcactg atcaatcgag taattgatgg agaaatgatc
    12781 tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa atgtgacagt agcaatctct
    12841 acggtaccaa gtaaatattc acaatctgct attgatgaaa ttgtcaaagg aaatgtaacc
    12901 gctatactta attttgcgcc tgacagagta actgtcccag cttatgtcaa tgttcaatac
    12961 attgatttga caaccgagtt gcagacgctt atttactttg atgagaatta ctcagaagtt
    13021 ttttcttagg aaaatttgag taggcttatt gatcttgcga gaactcgtcg taattctttc
    13081 aaaaattttt tagtactaaa tgaagtggtt ttttcacgac cacttgttga ctaaaaaaaa
    13141 aaaccattgt agaatgaagc ataacgctga acaagaggag taactggcac attcattctt
    13201 agagagagaa ctatctgctg aaaggttctc aatgacaacc agtgaaggta gcttgggagt
    13261 ttctgtcttg aatggagtag agatagacga gtcgtgatcg ttaactcact tgagaggcaa
    13321 taatgattga ttattgcaat ttaggtggta ccgcgtaaat catacgtcct agaatacgaa
    13381 agtattttag gacgtttttt agtttaaagg aggaaaacag catgacggaa gaaaataacc
    13441 tatcaacaaa atataatccg caagaagttg aagctggacg ttatcagcat tggttagaac
    13501 aagatttgtt taaaccgagc ggagataaaa aagcaaaacc ttattcaatc gtaatcccgc
    13561 ccccaaatgt tactggaaaa cttcatttag ggcatgcttg ggatacgacc ttgcaagata
    13621 tgctgattcg tcaaaagaga atgcaaggat atgatacttt atggctgcct ggtatggacc
    13681 atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct tagagagcaa gggatttctc
    13741 gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg ggaatggaaa gaagagtatg
    13801 cgagccacat ccgcgaacag tgggctaaaa tgggcttatc tttagattac agtagggaac
    13861 gttttacatt agatgatgga ttatctgaag cagtacgtaa agtttttgtt accttatacg
    13921 aaaaaggttt gatttatcgt ggtgaatata tcatcaactg ggatcctcaa gcacgaacag
    13981 ctctatctga tatcgaagtt attcataaag atatcgaagg cgccttttat catatgagct
    14041 atgaactagc agatggatca ggcgtagtag aaattgcgac aacccgtcca gaaacgatgt
    14101 tgggagatac ggcgattgct gttcatccag aagacgagcg ttatcaagcg ctcatcggca
    14161 aaaaagtgat tctaccttta gtcaataaac aaatcccaat cattgcagat gaatatgtag
    14221 atatggaatt cgggacaggt gtagtaaaaa tcactccagc acatgatcca aacgactttg
    14281 aggtaggtaa tcgtcatgat cttccacgcg taaatgttat ggatgatgat ggtacgatga
    14341 atgaactagc aggaaaatat gctggaatgg aacgttttgc tgctcgtaaa gcaatcgtgc
    14401 aagatctgaa agaaatcggt cgattgatta aaatcgatac gatgatccac agtgtgggtc
    14461 attccgagcg gacaggtgtc gttgttgaac ctcggctgtc tactcaatgg tttgtaaaaa
    14521 tggcgccgtt agcgaaaaaa gccattgata accaagaaac agatcaagca gtggaattct
    14581 tccctccaag attcaatcaa acgtttttac gttggatgga aaatgtccat gattgggtaa
    14641 tctcacgaca attatggtgg ggacatcaaa ttcctgcatg gtatcacaaa gaaacaggtg
    14701 aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa ctgggtacaa gatcctgatg
    14761 ttttagacac ttggtttagt tcagctttgt ggcctttctc aacaatgggc tggccagata
    14821 cggaaagcga agattataag cgatacttcc ctactagcac tttagtaacc ggttatgaca
    14881 tcattttctt ctgggtaagc agaatgattt tccaaagctt ggaatttact ggcgaacgtc
    14941 catttgagaa tgtcttgatt catggattga ttcgtgacga acaagggcgg aagatgagta
    15001 aatcattagg taatggaatc gatccgatgg aagtaatcga aaagtatgga gcagatgctc
    15061 tccgctggtt cttatcgaat ggttctgcac caggacaaga tgtccgcttc agctatgaaa
    15121 aaatggatgc ttcttggaac tttatcaaca aaatctggaa tgcttcacgt ttcgttatca
    15181 tgaatatcga aggaatgacc gtagaagaga tcgatttatc tggagaaaaa tcagtggctg
    15241 accggtggat ccttactcgt ttgaatgaaa cgatcgaacg agtaactgag ctatttgatc
    15301 gttttgaatt tggtgaagca ggtcgacaac tgtataactt tatctgggat gatttttgtg
    15361 actggtacat cgaaatgagt aaagaagtac tttatggaga tgatgctgtt tcaaaacaaa
    15421 ccacccgtag catcttagtc catacacttg atcagatcct acgtttattg catccaatca
    15481 tgccattcgt tacagaagaa atatgggaac atattcctca tcagggaaat tctctagtag
    15541 tggcagaata tcctgttgta cgtccagagt ttgatgatga aacagcttct aaagggatgg
    15601 aagtattgaa agaattgatt cgagccgttc gaaatattcg ttcagaagtg aatacaccat
    15661 tgtctaaacc aattacgttg ctaattaaaa caaacgatgc taaaatcgat caatttttag
    15721 ttgaaaatac aagctatatc gaacgcttct gtaatcccga agagttgacg atatcaagtg
    15781 agatcactgc gccagatcta gcgatgtcag ctgttctgac aggtgcagaa atcttcttgc
    15841 cgttagcagg attgatcaat attgaagaag aaatcaaacg attagaaaaa gaactggaaa
    15901 agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc taatgaacgt tttgtttcta
    15961 acgcgccgga agaagtagta gaggcagagc gtgcaaaaga aaaagattat ttagagaagc
    16021 aaacggctgt caaagaacga atcgaaagtt tgcgtactat tcaataagaa tagaaaaaga
    16081 gagtgtgaaa tcagctagct attactaaaa gctagctgat tgaacatcgt tacaggaaag
    16141 agtttgagcc tcactcttag cttcgattca taaggagatg tgacataaat tttgtcgcat
    16201 ctctttttta gtctactctt attatgtagg agaaggaggg aggtggatca tgtatcaaga
    16261 gtcaataaag tagttgataa aatcgttcct ctctctgttt cttttctctt tgtcatactt
    16321 ttatttttct tatgtgttct atttgttata ataaaagaga aatgggggaa ttgttgatga
    16381 cgattgaaga agcaatagaa tggatccata gtcgattacc atttggttca cgtcctggcc
    16441 ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc agagagaaaa gtaaaaacga
    16501 tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc ttatttgaga agcctattag
    16561 aagaggctgg gctgacagta ggtacattta cttcacctta tatcgaaacg ttcaatgaac
    16621 ggatcgcaat caatggcaaa agcattccag acaatgattt gatccagctt gtaaaaaaat
    16681 atcagccact gatcaaacaa cttgatcaat ttgaagcagt agctggtatt actgaattcg
    16741 agacattaac agcaatggca tttgattatt ttttgatgaa gaaagtggac gtagcgatta
    16801 tcgaagttgg cttaggcggc ttattggatt ctaccaatgt agctgtacca atgctgacgg
    16861 gaataacaac gattggactt gatcataccg aaatacttgg agatacaatt gaagaaatcg
    16921 ctgcacaaaa agctggaatc atcaaacaag gcgttccagt agtaacagga aatattgccc
    16981 cagaagcttt tactgtcatc caacaaacag ctaaagcaaa aaatgcaccg ctttatgtat
    17041 acggggaaaa ttataaaata aactatctgc atcctgatga gatgtgggga gaagtatttg
    17101 aattttttaa tgaaatagga aagatccctc aattaaccac tccgctttta gggcaacatc
    17161 aagctgaaaa tgcaggagta gccatcgaat tgtattttgt ttactgtcaa ttggaaaagc
    17221 tgcctttccg agaaaaagat gttttgaagg gattgaagaa atctcgatgg ccaggaagaa
    17281 tggagcgttt gagtaacgag ccattagttg ttttagatgg tgcccataat acgcatgcaa
    17341 tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg atatcaaatc aacattttgt
    17401 tttccgcctt acgaactaaa aatgttagag aaatgctaga acgattgcag caagtaccaa
    17461 atgtccatat ttatttgacg acctttgaac atccaaaagc catcgaatta agtgaagtat
    17521 cagatttagc aagtgagaga gtatcaatcg tttcattgtg gcaattcgga ttagcagata
    17581 tattggaaaa gatgacaaat gaagacgtat tagttgtgac cggttcactt tatttcattt
    17641 cagaagttcg ccatttatta atacaattag gaggagatca tgaagtataa tgcagtgatt
    17701 tttgacatgg atggtttgct gttcgacaca gagttagtgt attacgaagc cacacaagta
    17761 gtagcagaca agatgggttt tccttatgat aaagacctgt atttaaaatt cttaggtgtt
    17821 tcagatgaag aagtatgggc gaattatcat atgattttct cggaatttgg caaaacaagt
    17881 gtacaaagat tcatagatga ttcataccag gaaacgcttg aacgtttttc gcaaggagcg
    17941 gtacaaatga agcccggtgt gttggaactc ctctcttttt tagaagaaca tcagattccc
    18001 aaagttgtcg cttccagtaa ccaacgagca gtcattgaac ttttattgaa gaagaacaat
    18061 ctgtttgaac gatttgagac gattgtttct gcagaaaatg ttcaacgagc aaaaccagac
    18121 ccagaaattt tcttgttagc taatcagaag ctaggtacag cgaagaaaga aacattgatt
    18181 ttagaagatt cacaaaatgg tattttagca gcttctgcgg ctgagatccc ggtagtcatg
    18241 gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa aaacagctgg ggttgtttct
    18301 tctttacatg aaatccctgc attgttaaaa taaaaatcca tctcttttcg tatcttattt
    18361 tagataagat agaaaggaga tgtttttttg ggaaaacgat tgatcaaaga agtacctact
    18421 agctctttac caagggaacg tatggaaatt tatggcgcgg aagctttatc ggatcaggag
    18481 cttttggcaa tcttactgcg taccggtcaa cacccttata gtgtgatgtc gattgctgga
    18541 aatctgttaa aaacgtttgg aggactggcc tctttacgac aggctacttt gcatgagtta
    18601 gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa tcaaagcctt gatcgaatta
    18661 ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac cggttcgtac cagctacgaa
    18721 ttggcccagc aattgattct ggaaatgaaa gatcacaaac aagaacattt ggtttgtatt
    18781 tatttggaca cgaagaatca agtgatctta aaaaaaacag tgttcatcgg ttcgctgaat
    18841 cagtcagtcg ctcatcctag agaaattttc cattatgctg tacgttattg tgcagcaagg
    18901 atcgttttag cgcataatca tccttctggt aatgttattc catcgcagca ggatatgaat
    18961 tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg
    19021 atcatcggac gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa
    19081 gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga
    19141 atagaagttg aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt
    19201 agtgcttttg gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg
    19261 ctgaaaaagg atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct
    19321 cagctatcca aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg
    19381 ttgaagaatc agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt
    19441 aaatcaaatt gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac
    19501 ctgccacaag agacaatttc tcttgtggca ggtatttttt tgtatgaaaa agtaaataaa
    19561 tttaagaggc tatgaaagag ccaccttgta tcaaggaata agaacaaaca aaaatggctt
    19621 ctcatgtttt tcatttgttt tctcttatat tcaggacaag gtgctgattt cataacctct
    19681 tatttattct tcatctggtg tcattaccat tggcaaaatc attggatgtc tttctgtacg
    19741 ttcgaataag aaaggctgaa gcgcgccagt catcgcttct ttcaatttat attctgaaca
    19801 gtcaggtcca ttcaaacatt ctcttaaagc gtggaataag attcgctgag cttcattgat
    19861 catttctcct gattctcgca tgtagacaaa tccacgagag agaatatctg gaccagccaa
    19921 gatttctttt ttcttgatat ccactgtcgc gacagctaat acaagacctt cttctgaaag
    19981 caggtgacgg tcacgtaaaa cgacattacc aatatcaccg atcccgctac catcgatata
    20041 cacatctcca gccacaaaat gcccagcagg acgcgcagaa tcgctagtta acgctaaaac
    20101 atcgccattt tccatgatga agcagttttc tgcaggaaca cctacatctt gtgctagaga
    20161 agcatgtact ttcaacatgc ggaattctcc gtggacagga ataaaatatt ttggcttcat
    20221 caaacgaagc attaattttt gttcttcttg tccaccgtga ccggaagtat ggatgttatt
    20281 gatttttccg tgaatcactt ctgcaccagc ttcagacagc aaattgatca aatgattgac
    20341 acttgttgta ttaccaggaa taggtgaact tgaaaagatc actgtatcgt ctggctggat
    20401 ttggatttga cggtgagtac cattcgcgat gcggctcaac gcggccatcg cttctccttg
    20461 agaacctgta cataggatca tcgtttcatt ggcaggcaag cgattgattt cgtgtgcatc
    20521 tacaaatgtg ccgtctggaa ctttgatata tcctaaacgt tgaccgttga cgatggcatt
    20581 ttccatgctt cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg catctgctgc
    20641 ttgctgcaaa cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc cttcgatttt
    20701 tgaaaagatc ttcaaaatag aatgcccaat cgttttttct gatttagtaa atgttggaat
    20761 ctcagcattt gtactatcag ataaaaggca caagactcct tcttccccga tacgagccat
    20821 tttatgcaag tttgctggtt cgccaacagg agtgaaatcg aatttgaagt ctccagtcgc
    20881 tacgatattt ccagaaggtg ttttcaccac gatccctaat gcatctggga tactatgggt
    20941 cgttctgaaa aagctgacag aagtctttct aaaacggatg atcgtatctt cattgatctc
    21001 atggagctct gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga tcaacgctaa
    21061 tgcgagagga ccagcataga taggaatatt tgcttgtttc agcaagtagg gtattccgcc
    21121 gatgtggtct tcgtgcccgt gagtaatcac caaggctttt actttgtgta agttttgtat
    21181 gatgtagctg tagtcgggaa tgacatagtc aattccaagt aaatcgtctt ccgggaattt
    21241 gatcccggca tcaatcaaaa tgatttcatc ttggaattgt accccatacg tatttttccc
    21301 gatttcacct aatccaccaa tagcaaagac gcctgtctcg ttatttttga tggaaagttt
    21361 catattaaaa ctccactaat gagaaatcag cattttcttt ttcgtactct agatgacttc
    21421 cttctagaag ttgaacgaat tcaatattgt atggggtatt ttcttccaca cgttggcgag
    21481 cgtctacatc gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt ttagggttgc
    21541 gtacttttgt ttcttgataa taaactttat agatcattgt tttgtctcct ttatctgcgt
    21601 aaagcagtga tttagctagt tgattttttt agacaacaca aaacgagggg acaagccacc
    21661 tggtttttca tatgctttga ctagaatact ggattcgagt caaagagaca agcctcgttt
    21721 ttgcttatct gttcggatat tttttcaacc gtaccttctg tataaaaaat atacaacaat
    21781 taattgttat tctagcataa aacagcaaat aagtatagaa atcaactaga ttgaaagaaa
    21841 aaatcaatgt ttatcatccc gctataatca taatgacttt tttatgagag aagtgttcta
    21901 ttaataggct cgttgtgcta tcatttttag taatgaaaat gtaacaaaag attaaaagga
    21961 gatgagaaag tgaaactaat gtggcattat accatgcgtt ataagaaatt cttgtttttc
    22021 aatttcatat gcgtatttgg ttttattttg attgaattag gacttcctac gattttagca
    22081 cgtatgatcg atgtaggaat ccgcaatgat gattttgatt atgtgaagca acaagggata
    22141 tttatgatcg ttattactgt aatcggtatt attttgaata tcttgttagg ttattttggt
    22201 tcgaggatta cgactaatat cgtagcagat attcgagatg atttgttcga aaagatccaa
    22261 agttattcgc atcaggaata cgaaaatcta ggcgtatctt cattgatcac tcgtacaaca
    22321 aacgatgctt atcagatcat gttgttcctg caaaatattt tacgtatcgg ttttatggca
    22381 ccaatgatgt tcattgtcag tttgtacatg gtcatgcgta caagtgtgac actcagtttg
    22441 tatgtagtag gtgcattacc tttactatta ttggcagtcg tagcgattgc taaggtgtct
    22501 gaaccgttat ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat cttgcgggaa
    22561 aatctttcag gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga agaaaaacgt
    22621 tttgctgtag tcaatgagaa ttatgcttct agttcaaaaa gcttattccg tttgatggct
    22681 gttgcacaac cgggttttta tttcttattt aatatcgtaa tggtattgat catctggagc
    22741 ggggcagtgc aaatcgatgc aggcgagctt cgtgtaggag atttgattgc gtttatcgag
    22801 tatatattcc atgccttgtt ctcattcatg ttatttgcta gtgtgttcat gatgtatcca
    22861 agagcagctg tttcagctgg acgtatccaa gaagccctag atgccaaacc ttcgattgta
    22921 gaagatccaa atggtatatc tgatacaaaa actaaagggt atatcgaatt tcaaaatgta
    22981 acatttgctt atccaggcca ttcgcaagag ccagtgatac gaaatgtcag ttttacagcc
    23041 tctccaggag agaccgttgc ttttattgga agtaccggta gcggaaaatc tacgttgatc
    23101 caattgatcc ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga tggagtagac
    23161 gtgagagatt atcggctaag caagttgcga gataagattg ggtttattcc gcaaaaagcg
    23221 ctgctattta ctggaacgat tgcagataat ttgcgatacg ggaaagaaga tgctacgcaa
    23281 gaagaaatgg aacgtgcagc tgagattgct caagcttcag aattcatttc tcgaaaacca
    23341 gatggattta atgaacattt atcagaaggt ggttccaatt tttcaggtgg acaaaaacaa
    23401 cgattggcaa tagccagagc catcatacgc cgtccggaaa tctatatttt tgatgacagc
    23461 ttttctgcat tagattatca aacggatgcg aagttacgtg ctcgtctgaa aaaagaaacg
    23521 acagaatcta ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca tgcagataag
    23581 atcatcgtat taaatgaagg agaagtcgtc ggtatgggga cgcatcggga gttgttggaa
    23641 aaatgcccga tttattatga tattgccgct tctcaattat caaaggagga attagcatga
    23701 aaaaaacctt ttcttcctta aaacgtttag ctcgatatat cagaccgtat cgtgagactt
    23761 ttcttttagt gatcctattt acagttctga cagtggcttt caacgtggct ttgccatatg
    23821 ttacaggact tcctactaca gaaatcagcc gtaatattgc caatggcgaa tcaatcaatt
    23881 tttcttatat attcaaatgt ctcatttgga tcacagttgt tggaataggg tattgtattt
    23941 cgcaattact ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg catgatttga
    24001 gaagagacat agaagaaaaa atcaatcgtc ttccggtttc atatttcgat aaaaaccagc
    24061 aaggaaatat tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt gctttacaac
    24121 aagcttttat tggtatcgta aatgcgatat tgggaattac gttggctgca gcaatgatgt
    24181 tttatatcca gccgatgatg gcgctgatct ctatgatcat gattccaaca tcgatctgga
    24241 tctcaaaacg agtgatcaat gcttcgcaga aatattttca aagcatgcag aattctcttg
    24301 gtgagctaaa cggctacgtc caagaaaata tgacaggttt tagtgtattg aaagtatatg
    24361 gacgcgaaaa agaaacattg gaaggattca aaaaagttaa tcacagcttg aaatactttg
    24421 ggtttcgtgc agcattcatt tctggcttga tgatgcctct agttcaatta actgcctatg
    24481 ctacttatat tggtatggct gttctaggaa gcttttatgt tattactggg gcgatcgttg
    24541 tcggtcaact acaagcattc atccaatata tttggcaaat cagtcagcca atggggaacg
    24601 tgacacaatt gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt gtattcgaga
    24661 ttttagatga gccagaagaa aaagaaaaca aagtagatgc ttcactgcca gaaacgattt
    24721 taggagatgt gacattcgat catgtcgatt ttgcatatga tccgaagaaa ccattaatcc
    24781 aagatttgaa ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt ccgaccggtg
    24841 ccggcaagac gacattgatc aatctattga tgcgttttta tgacgtagac aaaggagcaa
    24901 taaggattga tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt tcgctatttg
    24961 gtatggtctt gcaagatgca tggctttatc aaggaacgat tgcagataat atccgcttcg
    25021 gaaagcttga tgcgacagat tatgaagtag ttgatgctgc gaaaacagca aacgtagatc
    25081 attttatccg aacgatgcca gatggatatg atatggccat caattctgag ggagacaatg
    25141 tgtctttagg acaaaaacag ttgctgacga ttgcccgagc agtggtctcc gatcctaaaa
    25201 tcttgatttt agatgaagca acaagttcag tagacacccg tttagaagcg ttgattcaaa
    25261 aagcaatgga tcgcgtgatg gagggacgga caagtttcgt catcgctcac cgtttatcga
    25321 caatccgtga tgcagatctg attctagtga tggatcaagg tcagatcatt gaaaaaggaa
    25381 cgcatgagag tttattggca caaggtggat tttatgaaaa actgtataac agccaatttg
    25441 ctgaggaagc agaataagtc atataagaat aaaacagttt aaaacttatt ttttgttctc
    25501 tattcttgat aatggtaaag taaagttatc aagaaaagtg aggacacgga gatggaacag
    25561 tttttaaatg aaaaaattac tgaagccaaa agagaagttg atcgtttaac gcacgtctgt
    25621 cagacgaagc cttttgattc agctgatttt tttgaaaatg agtttcaaat cgaacgcttg
    25681 gcagcacaaa tagaagtgta cgaattaatt ttaaaaaaag taatagaaga aaatatagat
    25741 gtacaataaa aacaggtaaa acgtcagtta ccagatactg taaatagaca tctggcctga
    25801 gcaggtttta cctgtttttt ttcttatgaa ataaatatat gctatgatac ctttattcaa
    25861 taggattagt ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg tttatagcat
    25921 caaatgccac agtgattggt gatgtgacat tgtcagaaga tgtgacgatc tggtatcaag
    25981 ctgttttacg cggggacagt aattggataa aaataggaca aagaacgaat atccaagatg
    26041 gtacaatcat ccatgtcgat catgatgcac ctgtagatat tgcggagaat gtgacggtag
    26101 gacatcagtg catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc gggatgggga
    26161 ccacgatatt gaatcatgcg gttattggtg agaatagttt gattggtgct ggatcattag
    26221 taacagaagg aaaagttatt cctcctaatg tcttggcttt tggtcgccca gctaaagtga
    26281 tccgtccttt gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag cattatgtcg
    26341 agataggtcg gatgcatgcg caaggaaaat tcaaggagca aagataggca gtagcatcca
    26401 taaaataaaa aaggatggtc agtttgagtt gaccatcctt ttttatttgg tttagataag
    26461 agagcttaaa aaagcttcct ttgaaacaga ttaatttaat gaagaactgt tttccatgac
    26521 ttcatcgatc aatccatatg cttttgcttg ttcagctgtc atatagttgt cacgatctgt
    26581 atctttttca atcacttcaa gcggttgtcc agtgcgttca gctaaaattt tgtttaagcg
    26641 ttcgcgtgtt tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt gcccttgagc
    26701 gccgccaaga ggttgatgga tcatgatctc agcatttggt aaagcaaatc gtttaccttt
    26761 tgtaccagct gtcagcaaga agcttcccat tgaagcagcc atacccataa caattgtttg
    26821 gacatctgct ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg taactgaacc
    26881 accaggagaa ttgatataaa gatagatatc tttttcagaa tcttgagcgt ctaagaaaag
    26941 caattgggcg atgatcgaat tagcaagatc atctgttact tggccgctta acatgatgat
    27001 acggtctttc aataaacgtg agtagatatc gtaagctctt tcacctcgtg aagattgttc
    27061 aatgactgta ggaattaaat tcataataga gttcctcctt gatttcgttt agaaatagtg
    27121 taccataatt tagtttctaa tacctaaaat gtagtataca tttttggtca aaaaaggtca
    27181 aataaaaaac ttaggcaaat tagtcgcata aacccgcttt atggtatgat aagcagagaa
    27241 acaaaacaag gaggaacaac agattgatag ttggtattac tggccttatc ggagtgctga
    27301 caggtgctgt agttgtctat atcggcatga tactgcaaat gcgtcacttg gaaaataaag
    27361 aacagcgtag acgtgaattg gaattgaaag tcaaagaaat cgagacgctg aatcaattaa
    27421 ataaaaagat caacgagatc ttgcaaaaac ggacaacgct gatggaagaa tatgtcagct
    27481 ttgatgcatt tgatgattgt tatatcacga ttgacgattt tgcgtatctc caatcatttt
    27541 cagcgcaaaa tagtttttat ctgccaaatt atattttaga cgagctattt aagaatatct
    27601 cccatcgaaa agtgatttta tctccagatg aaacaatgaa gattggtgga tatgcttata
    27661 aaggcggacg cgtgatttta gagaatttta cggatagtct gactgaaatg atcaatgaaa
    27721 aaaaagctca gttgaagact atgacgcgcc agccgattgg tttcttctcg aaagatgatg
    27781 tttggagctg atattttgaa gcattgccgt tgcacatgag agcgtggtaa tgctttcttt
    27841 ttttcttaat gatacaatag aactgaacgg agtgaagaaa gatgttacaa atctatgccc
    27901 atcgcggaag cagcggcacc cacccagaaa atacgcttcc tgcatttgca gaagctgtac
    27961 gagtaggtgc agatggtatc gaactagatg tccatctaag caaagatggt tatttgatcg
    28021 ttatgcacga cgaagaagtg gaccggacga ctaacggtaa agggctgatt cgagaaaaaa
    28081 cattggaaga actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa tttccagcag
    28141 ctaaagttcc tgaattgaga gaagtgttag gactgctcct ccaaaaaaat taccgaggga
    28201 tgctaatgat tgaaattaaa actgatcagt atgaatatcc cggtatcgaa gaaaaattag
    28261 ccgaaacatt aaaaagtaga gattggcctt ttcaacatgc atattgcagc tttcatttgg
    28321 attctttgga aaaaatcgca ttactggaac cggagataca gctggactta ttgatgagta
    28381 cttctgataa aaagatccaa ctagcagcag aaacccctta tatagaagga gtacatccaa
    28441 aaaatacttg gttgttcgaa catccagtcc aggcagctga ttttccaaaa gcaattcgtg
    28501 catggacgat taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa atagatggaa
    28561 taataactga ttttccggag aaagctcttc ggtggaaaca atcaacgaaa agtaggtaaa
    28621 atatggaaaa agtattagtg atcgtcggtc cgacagcagt cggtaaaaca gcgctaagtg
    28681 tggaactcgc acaactattt ggaggagaga tcatcagtgg tgattcattg caaatctata
    28741 aaaatctgga tatcggtaca gcaaaaatca aagaagaaga gatgaataat gttccgcatc
    28801 atttgattga tgtgatcgaa cctactcaac gttactcagc cgctgatttt cagcattcag
    28861 gtcgacaatt gattaccgag attgcgaacc gagggcacct acctatcata gctggtggta
    28921 cgggtctcta tatccaatcc cttctatatg attatcagtt aggagcgaca gaagaagcga
    28981 atcctgagat tagaaaaaag tatgaaacgt tagctgaaac aattggttcc caagcattat
    29041 ggaatcagtt gaaagaaaaa gacccggcag ctgctgagaa aatccactgg aacaatcaac
    29101 gcaaagtgat aagagctctg gaagttttag agacgacagg acatagtatc acggcaccta
    29161 aagaagagcc aaagcaattg tatgattacc tcatgattgg attagatacg aagcgtagta
    29221 ttctttatga acgaatcaat caaagagtcg atttgatgct agcagaaggt ttagtagcag
    29281 aagcgcgtaa ggtgtatgag cttggagaag tccaggccag tcaaggtatt ggatataaag
    29341 aatttttccc ttattttgaa gggaaagaaa cattggaaga agcaacagaa caagtcaaac
    29401 tacattcgag aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt ttggatgcac
    29461 agtggtttga tcttataagt gagccaaatc aaaaaagtca gattgagcaa acaatcaaaa
    29521 aatggctgga ggcggaataa aatggaagaa aaagtgatca tcgtaggggt agaaaccgaa
    29581 gcaaaccagc gttacttttc agagtcaatg gaagagctag ttcagctgac gaataccgca
    29641 tcaggagaag tcgtattcac aattacacaa aagagaccac aagtggatcg gcaaacgatt
    29701 atcggtaaag ggaaattaca agaattagtt caacaagcgg atgcgcatga agcggatttg
    29761 atcatcttta atcatgagct aactccgaga cagagccaac tgattaccga tgctgttggt
    29821 cttccggtaa ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg tgctcgttcg
    29881 aaagaaggga agctgcaggt tgaacttgct cagttagaat acctgctgcc tcgtttagta
    29941 gggcaaggga aaacattatc tcgtcttggc ggtgggatcg gaacaagagg gccaggtgaa
    30001 acaaagttag aaacagaccg ccgacatata cggaataaaa tttttgctgt taagaaagaa
    30061 ttgaaagaag tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa tagcgagatt
    30121 tttcagattg gacttattgg ctacacgaat gccgggaagt caactatctt gaatctattg
    30181 acacaagcgg atacttattc gaaagatcaa ttgtttgcaa cgttagatcc attgactaag
    30241 agatggcgtt ttgctgaagg atttgagatt actgtaacgg ataccgtcgg gttcatccaa
    30301 gacctgccta cgcaactgat cgatgctttc cattctacat tagaagaaag tcaaaatatg
    30361 gatcttttgc tacatgtagt cgatgctagt tctccagatc gtattttaca ggagcagact
    30421 gtcttaaaat tgatggatga actgaatatg aaagaaatgc cgatactgac tgtttacaat
    30481 aaggcagacc agatcgaccc agcgatgttc acgcctacgc tattcccaaa tgtattgatt
    30541 tctactcaga cgaaagaagg taagatggcg ttgatcgaag caatcaaacg tcagctgatg
    30601 gaactaatga ccccttatac actttctgtg ccaagcaacg aaggacagaa attaagcgag
    30661 ttgagaagac aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa cgaatatgaa
    30721 gttaaagggt ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata aaattttatt
    30781 tttttaa
//
LOCUS       DARBER010000020        29137 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARBER010000020 DARBER010000000
VERSION     DARBER010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.020"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="SCK06_000923"
     CDS             complement(<1..1031)
                     /locus_tag="SCK06_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1150945.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="SCK06_000924"
     CDS             complement(1113..1802)
                     /locus_tag="SCK06_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1150946.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="SCK06_000925"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="SCK06_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG1150947.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="SCK06_000926"
     CDS             complement(2329..2832)
                     /locus_tag="SCK06_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG1150948.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="SCK06_000927"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="SCK06_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG1150949.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="SCK06_000928"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="SCK06_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG1150950.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="SCK06_000929"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="SCK06_000929"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG1150951.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="SCK06_000930"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="SCK06_000930"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG1150952.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="SCK06_000931"
     CDS             complement(6444..7463)
                     /locus_tag="SCK06_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1150953.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="SCK06_000932"
     CDS             complement(7636..8541)
                     /locus_tag="SCK06_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1150954.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="SCK06_000933"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="SCK06_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG1150955.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="SCK06_000934"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="SCK06_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG1150956.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="SCK06_000935"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="SCK06_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1150957.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="SCK06_000936"
     CDS             9754..10398
                     /locus_tag="SCK06_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1150958.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="SCK06_000937"
     CDS             complement(10454..10957)
                     /locus_tag="SCK06_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1150959.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="SCK06_000938"
     CDS             11554..12519
                     /locus_tag="SCK06_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1150960.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="SCK06_000939"
     CDS             complement(12618..13790)
                     /locus_tag="SCK06_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150961.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="SCK06_000940"
     CDS             complement(13777..14922)
                     /locus_tag="SCK06_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150962.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="SCK06_000941"
     CDS             complement(14936..15868)
                     /locus_tag="SCK06_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150963.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="SCK06_000942"
     CDS             15985..17064
                     /locus_tag="SCK06_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1150964.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="SCK06_000943"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="SCK06_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="SCK06_000944"
     CDS             complement(17789..18016)
                     /locus_tag="SCK06_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1150965.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="SCK06_000945"
     CDS             complement(18119..20707)
                     /locus_tag="SCK06_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG1150966.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="SCK06_000946"
     CDS             complement(20727..21386)
                     /locus_tag="SCK06_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1150967.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="SCK06_000947"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="SCK06_000947"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG1150968.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="SCK06_000948"
     CDS             complement(22159..23025)
                     /locus_tag="SCK06_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1150969.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQLVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="SCK06_000949"
     CDS             23134..23775
                     /locus_tag="SCK06_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG1150970.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="SCK06_000950"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="SCK06_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG1150971.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="SCK06_000951"
     CDS             complement(25216..26115)
                     /locus_tag="SCK06_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1150972.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="SCK06_000952"
     CDS             complement(26117..26914)
                     /locus_tag="SCK06_000952"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG1150973.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="SCK06_000953"
     CDS             complement(26892..27596)
                     /locus_tag="SCK06_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG1150974.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="SCK06_000954"
     CDS             27727..28302
                     /locus_tag="SCK06_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG1150975.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="SCK06_000955"
     CDS             28420..29097
                     /locus_tag="SCK06_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1150976.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8578 a         6170 c         5240 g         9149 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgctttaaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacaagt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    29101 aggctctttg tcaaatgatg ttggtaaaga gaaatct
//
LOCUS       DARBER010000021        28050 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARBER010000021 DARBER010000000
VERSION     DARBER010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.021"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(598..1542)
                     /locus_tag="SCK06_000956"
     CDS             complement(598..1542)
                     /locus_tag="SCK06_000956"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1150977.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1838..2782)
                     /locus_tag="SCK06_000957"
     CDS             complement(1838..2782)
                     /locus_tag="SCK06_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150978.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            3003..4217
                     /locus_tag="SCK06_000958"
     CDS             3003..4217
                     /locus_tag="SCK06_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1150979.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4239..6254
                     /locus_tag="SCK06_000959"
     CDS             4239..6254
                     /locus_tag="SCK06_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG1150980.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6337..7710)
                     /locus_tag="SCK06_000960"
     CDS             complement(6337..7710)
                     /locus_tag="SCK06_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1150981.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7736..8020)
                     /locus_tag="SCK06_000961"
     CDS             complement(7736..8020)
                     /locus_tag="SCK06_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1150982.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(8022..10037)
                     /locus_tag="SCK06_000962"
     CDS             complement(8022..10037)
                     /locus_tag="SCK06_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1150983.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10216..10521)
                     /locus_tag="SCK06_000963"
     CDS             complement(10216..10521)
                     /locus_tag="SCK06_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG1150984.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10610..11641)
                     /locus_tag="SCK06_000964"
     CDS             complement(10610..11641)
                     /locus_tag="SCK06_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1150985.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11848..12723
                     /locus_tag="SCK06_000965"
     CDS             11848..12723
                     /locus_tag="SCK06_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1150986.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12875..13717)
                     /locus_tag="SCK06_000966"
     CDS             complement(12875..13717)
                     /locus_tag="SCK06_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1150987.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13809..15686)
                     /locus_tag="SCK06_000967"
     CDS             complement(13809..15686)
                     /locus_tag="SCK06_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1150988.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15683..17104)
                     /locus_tag="SCK06_000968"
     CDS             complement(15683..17104)
                     /locus_tag="SCK06_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1150989.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17107..20496)
                     /locus_tag="SCK06_000969"
     CDS             complement(17107..20496)
                     /locus_tag="SCK06_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG1150990.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20753..22144
                     /locus_tag="SCK06_000970"
     CDS             20753..22144
                     /locus_tag="SCK06_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1150991.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22217..22864)
                     /locus_tag="SCK06_000971"
     CDS             complement(22217..22864)
                     /locus_tag="SCK06_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1150992.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22872..23558)
                     /locus_tag="SCK06_000972"
     CDS             complement(22872..23558)
                     /locus_tag="SCK06_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1150993.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23564..24637)
                     /locus_tag="SCK06_000973"
     CDS             complement(23564..24637)
                     /locus_tag="SCK06_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1150994.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24859..25533
                     /locus_tag="SCK06_000974"
     CDS             24859..25533
                     /locus_tag="SCK06_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1150995.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25538..26878
                     /locus_tag="SCK06_000975"
     CDS             25538..26878
                     /locus_tag="SCK06_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1150996.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(27027..28013)
                     /locus_tag="SCK06_000976"
     CDS             complement(27027..28013)
                     /locus_tag="SCK06_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG1150997.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8204 a         5275 c         5080 g         9491 t
ORIGIN      
        1 taatcataca aaatagtttt ttcataaaaa tatggaaaat atggattaat tttgaaataa
       61 ttgatttaaa aggaatacaa aagagtgaat agtaggaaat agtggtttag aactagtggg
      121 ctgatttttt atatatttta gtttacataa tgtatattat acaaagtaga tttaaaaaat
      181 gataaacctc tctcgaacgg ttttttatca aaaatcgtca tttttccatc aaaatctgct
      241 tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt ttaattgaat
      301 gataaaaacc agtttcttta ttcagttaat caaatatgaa cgaattagat atgaatcatt
      361 gtatttgccg ttgcggaagg attttttaat taaccacaga taaacattga taacgattcg
      421 cttattgtaa tcaacagatt aaatttcata tgttcatatg tacggataca ttttcggacc
      481 gattaatttt aaccattctg tttatttgct tacctattag atatcaataa aaaactgctt
      541 aacagctatt tcagctgtta agcagtttta ggatttttag ttcatattca ttgtttctta
      601 taatacagtg ttcaatactt ctcttaaagc acttgttgat ttgctgaaca ttgctttttc
      661 ttcttcgttg attgataatt caacaacttc acgaacacca ttttcgtcaa cgattgatgg
      721 cacacctgta aagatatctt tttcgtcgta ttcaccagtt aaataagcag atacaggcaa
      781 tacagcttgt tcgttattca agatagcttt tacgatacgt gcagtactca taccaatacc
      841 atagtaagtt gcttttttac gatcaatgat ctcataagca gcattacgga ctttatcagc
      901 aatcacatct aattcatctt gggcaatacg atggtctttt tctacaattt caaacactgg
      961 tttaccaccg acagttgtat gtgaccatgc agcaacttct gaatcaccat gttcacctaa
     1021 gatatagccg tggacgctgc gtgggtcaac ttttaatttc aatgcgattt ctttacggaa
     1081 acgagttgta tccaatgttg ttccagtacc gataacacgt gatgttggca gaccagattc
     1141 gttccaagcg atatatgtca aaatatctac tgggtttgaa gcaacaacga taataccgtc
     1201 gaatcctgat cccatgattt cttttacgat ttggcgcatg attgacgcat ttgttttaac
     1261 taaatctaaa cgagattgac caggtttttg gttgatacca gctgtgataa caacgatatc
     1321 agcatcttta cattcttcat agccacctga ccatacagcc acattttcat cgccccaagc
     1381 cataccatct aaaaggtcca atgcttcccc ttctgctttt tcttgattca catcgatcaa
     1441 tactaattca ttagagattc cttggttgat cattgcataa gcgatacttg ttccaacaaa
     1501 acctgtaccg acgattacta ctttacgact tgtttttttc atgaataaac agctcctttt
     1561 cataactatt tgttactttc atgaaaagta tagtcttgtt tattcatttt atcaacatgt
     1621 taattataat agttgttatt ataatttttg atggaaacga attcctgaat acgcataaca
     1681 gataaatgac aaatgtttac acctatatcc cctgataata caaccaaaag tttagaactg
     1741 aaaataaatc caaaaaaatt cgctgcaacg atataataag acagcaatcg cacctaaaac
     1801 aattttcctt catatgcttt agtgaaagat tttcttctta atgtttattt ctccattcat
     1861 tgggagtaca tgttgaatac ttcttgaata atttgaataa agaagtcgtt tgtttatatc
     1921 cgatacgatt agcgatttct tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa
     1981 agtcgaattt tttctttaga accagttctt gaaaggaagc atcaaaatac attttcaatt
     2041 tattactaag atagaccgga ttgtagccaa aatgagctgc tgtttctttt aacgtacaag
     2101 aagtaaaatg cttatcgata taatctaata ctctaagcat ctcattttct gcgcatccat
     2161 ctgctgatga aaagctttgt tgttctaata aatgagtcaa ttcgatcagt aatagtgatg
     2221 tatagagatt taacgaacgg atcttatgac tgtatgtatc aaaataaatc aacagcatat
     2281 tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat
     2341 gttccccttt ttcttttgat gcatttatca aaaaagaaga aagtagatta tttgtgcttg
     2401 ccaaatgatt caatatctct gtagataagg tttcttcttt taataagatg ttgattaaaa
     2461 tatcctcttg ccctgtgtat tcgattcttt gagtagcttc tttatcaatg atcaccacgt
     2521 cactttcttc catctctatc ctttctccat taataatttg taaacatgta ccgctataca
     2581 tataattgat ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt
     2641 tactgatatg aatatttccc tgttgaaata gatcccctcg gtggattttt ggcggtgtct
     2701 gaagatggta agctaagttt aaatcattga tattcattcc ttgttcacgc tgcagagttt
     2761 cgattgattc ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa ttgactccct
     2821 cctttttgta agcggtttct ttcgcttatt agttcacgct gtactgctat tatatgcaaa
     2881 gctatttttg gtttcataaa gactttctat ggttgggaca tgaacttcct gtgattccgc
     2941 tattgagaga ataaccactt catactatat ttttgataag cgcttacaaa aatgaaagga
     3001 acatgcttat gaataaaaag atgatgaaat tttctctgct gtcagtctct cttattttga
     3061 cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc agctacttat gccacgcgtt
     3121 ctttatctgc tgttgaatta ttaacgactg ctccctcttt tatggtcact atttttgttt
     3181 tgattagtag ttttattgct aggaaaatcg gacaaaaaca aactgtgatc atcggattag
     3241 tgattgcatc gatttgtggt atcctccccc tcttctcaca aagttacagt attgtcttag
     3301 cttcaagagc tggactaggt attggctttg gattgatcaa ttctttggca gtcagcatga
     3361 ttgctgcctt ttatcacgga gatgaacgag cctctatgat tggttttcaa agtgcatttc
     3421 aaggatttgg tgcagcttta atgacatttg ttgcaggaca gctagtacag ttcggttggc
     3481 aatatacctt tttagtctac gccatcagct tacctattct tgtcttgttt gttttgtttg
     3541 ttcctacacc tgaaacaact gaacttcaat cgcatggcac agccccaaca agacaagtaa
     3601 tcaaaacgaa tcctaaaatg attagttatg gtatttttct cttgctggtc atcgtggtat
     3661 ataatgctgt atcgattaaa ttagccacag tcctgacgac ttattctcta ggaaacgaaa
     3721 ctaatgcagc gaatatctta acaatcatgc aattggcaag tatggctgcc ggagtttgtt
     3781 ttggctggct tcagacaaaa gcacatcgct atatgcttac actttcttta ctactgatgg
     3841 gaatcggctt tgtattgatt gccactgtga taaacctgta tgtgattggg tttggtgcga
     3901 tcctaacagg aatttctttt tcgttattca ttccttactt attcaaccaa gtaagtatcc
     3961 aagcgcccaa gtacgcggag agtttcaata cttcaatctt attggttggc gctaatttag
     4021 gtactttcat tgcaccatat ggtttgctct ttctctcttt tgcttctctt gggagtaaaa
     4081 cacttgctgt tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt attagccttc
     4141 tagtagtaca caggacgaat agaaagacag tgataagagt agatgaagaa ataaaagaac
     4201 aattgacaac aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag
     4261 atagatggct ttggtatcct ggagactttg agatccgcca tggactgcta caaaatttcc
     4321 aacgggaaga acgaggtttt gactggccag cgtactggta catggatgat tgccatcgga
     4381 acgtcaagtt taagagatat tactttcttg atcaaccttc catgtttaaa gtgacgatcc
     4441 aaggagttgg gtatgtagaa attaatggac aaaaacatcc atgtggaaaa tggctgactt
     4501 gtccagctgg taaagcaaaa attcgtatct ttgtaggaca tacatcaggg ctaccagcga
     4561 tgtttatcga gggagacgaa gtaaaatcag atattggctg gacagcaagt aattttattg
     4621 aagaatatcc agctggctgg agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa
     4681 tctattatca aaaagaatgc gtccatccag tcactgaaac ggagattggc ggaggaatct
     4741 tattcgattt tgggcgagca gtgaatggct gtgtagaact aatatctctg gatgaccata
     4801 tcgaatctat cactctatgt tatggagagt ccgatgtaga agctatggat atcgattatt
     4861 gctactataa gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct
     4921 atttgtttat accagaggtt tctactggaa tggttcaact tcaagttttt catgaatttt
     4981 tatctctttc gaaacaagct gctttttcaa gcgattcttc tttactgaat cagatctggt
     5041 cagtatcaga agaagccttc aagttatgta gtgatttgtt ttttatcgac ggtatcaaac
     5101 gagatcgctg gatttggtct ggagatgcgt atcaatcatt catgatcaat caatatcttt
     5161 tttttgacga ggaaatcaat aaacgaacga tattagcatt aagaggacaa aatgaaatca
     5221 agcagcatat gaatactatc gttgactatt cgatcttgtg gttgattggt atcgaaaacc
     5281 actatatgat gacgaaagac tatgaatttt tacgcatcgt ctaccctaag atgcaatcat
     5341 taatgagata cttgatggag caaacgaatg aattagggtt tatttatggg agagcaaaag
     5401 attggatatt tattgattgg tctgaaatgg ataaagaagg aacgcttgct gcagaacaag
     5461 tcttattact gaaagcctat cattccatga tcacttgcgg agatatctta gctgaaacat
     5521 atggagagaa cgaaaattat catactcatc tctatcaaga aaaatatcaa ctattgaaac
     5581 aaaacctatt tgactacttt tgggatgaag aacttggcgc ttttatcgat tgctatgaat
     5641 ctgggagaag aaatgtgacg cgtcatgcga atattttcgc tgttttattc gattttgcta
     5701 acaaagacca acagcaatcg atcttaaaga atgtcttatt gaatgacact atcacacaga
     5761 ttactactcc atatttcaaa ttctatgaac aagatgcttt atgcaaactc ggacagacga
     5821 atatcgtcta ccaaacgatt cttgattatt ggggagggat gttatctcat gatgcggtaa
     5881 ctttctggga agaatacgac cctactcaaa caggaaatga aaagtacagt atgtatggag
     5941 atccttatgg aaaaagctta tgccatgcat ggggagcaag cccaatctat cttttaggac
     6001 gctattttat cggtttgtat ccaacaaagc caggttatga agaatttatg atacaaccta
     6061 atctagattc atttgaaaca ctgaattgta cgctacctat caaagatgga aagatttcgc
     6121 ttgaaaaaac aaaagataag atcgtgatta cttctgatag aacaggcgga acagtgaaaa
     6181 tgaatggtaa aagttatcct cttctagcaa aacagccact tgtcttaaca agctattcaa
     6241 aaataaatgc ataaaacaaa aaactgcttg cgacatatca gaagacttta aatcttcttc
     6301 tgatattgtc caagcaattt ttattttgaa gtttgcttaa ttctgttctt tagaaacaat
     6361 gtttgttctt tttccggcaa aaagaaagct agataatacg acaagtaaaa agaaagcgat
     6421 gattccaact gtcaaggcta tttgaccgta ttctcctagt ttcccaagta caagtcctgg
     6481 tactagataa tcagtatcgg caaatgtaga agaagcgatt ttaagatctc ctaatactgg
     6541 aaggataaat aatggcagcc aactaatcaa taagctattg aaagcagaac ctaataccgc
     6601 ccctcttcta ccgccagtcg agttgccgaa gacaccagaa gcagctccgc agaagaaatg
     6661 tcctactacc ccaggaatga taactgtagt ttgcaagcct atcatagcaa acatggatag
     6721 tgttcctaca aagaagctaa caaaaaagcc aattaataca gcattaggtg catatggaaa
     6781 aacgatagga acatctaatg ctggtttaga atcgggtacg agttttttag caatcccttg
     6841 gaatgctgga acgatttctg ccaacaccat tcgaacccct tgaaggacga taacaaaccc
     6901 agctgtgaat gttccagctt ggatcagtgc aaagattaca gcgttgtctc cattgcttag
     6961 ttctctttca acaaatccag gaccagctaa caaagcaagt gcgacatata cgatcgacat
     7021 caagagcatg atcgatacag tagaatctcg caaaaagctg aaacttttag gaatctttat
     7081 gtcctctgta gaacggtctt tgtttccaaa aaactttccg atccatccac tagaagcata
     7141 accgatattt ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa
     7201 cggttgacaa agagcaggcg taatagttaa tagtgttcct tcgaagaccc cgcctaataa
     7261 gatcatccac aaatttgcag taaaccccgc gcttactaaa atgactgctg ttagacaaga
     7321 aacataaagc attgcttgac ctgtaagaaa aatgaattta tatcttgtga atctagcaag
     7381 taaaatattc acgatcatcc ctactagcat gatcaaagca gtaggtgtac cgtattgttg
     7441 caaagcaatc gccactactg cttcattgct aggaactact ccttgagtat gcagagctgt
     7501 ttggaacatc gaagcaaaag gggttagtga attactgatt aaaccagcac cgccgatcag
     7561 tactaggaat ccgacaaatg ttttgatagt tccttggatc acttctgaaa atttcttctt
     7621 ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg atggagggct gactgacgat
     7681 tgacacaaac aaattcaaca atttatccat tgatagttcc tcccttgaat catttttatt
     7741 cttttgacaa gatcttcgtt actttttctt ttacttcttc gctgtcaatg atcgattgca
     7801 aaggtacgag cttttcatca ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga
     7861 agaaataatc agccatatcg ctagatacac ttccaacatc cgtatgactt acttcaaact
     7921 cgtccacatc tgctccaaga tctgcaagtg cttgtttgat gttcatctct accataaaac
     7981 ttgttcctaa tccactttga catactgcaa aaattttcat atcattcgtc tccttttctt
     8041 atgatcgcta atatttcttc ttttgttgat gcgtgtaata aatcttgaag attttgttta
     8101 ttagaaagta gctttgttaa atttgccaaa gcccgaagat gtgcctcatt atcgatagca
     8161 gctaaacaga tgaacaaact aatgggatgg gctggattat caagcagatc cacaggttgt
     8221 tgaagtttca gcaaagacat ccctacttcg tttacgccat cttctggacg agcatgaggt
     8281 aatgcgatgt gatcgccaat atggataaac gcaccatact gctccacgcg ctcgatcata
     8341 gctttgatat aactttcttc tattttattt tctgataata gcggctgtgc ggcaaatgtg
     8401 attgcttctt gccagttttt tggttgatcc gataattgga tcatttcttc tgttaataat
     8461 tcagatagca taggacgttg atcctccttc ttttctaaaa gtttgttcat tttacgattt
     8521 aatacttgat agagcttttc ttcgtctacg ccttctttta attcgatata aggcagtagc
     8581 gccgacaaca attcttgggc agaaggcatt gaaaatcctg gtatcagcca atctctttgt
     8641 acttgattga tcagctgatt tttttccaag tcagtcatga ttggatgcag aacatagaga
     8701 gactttttct tatcagtaag tgtgagaggt acagtagaaa aaataatatc gtaagaagtt
     8761 tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat catcgggaat
     8821 aattgctgaa gttcactttt taagatcaat gaagagctaa tgccacttgg acagacgatc
     8881 acggctctta tttttctatt tctttcttct gcgtccgctt ttctgatctc tccgccaaat
     8941 agtatagtga aaaaaccaat ctcttcttca gggatgctct tccctattcg tctttctaac
     9001 ggaagtaatg cttttcttgt catttcgtag atggaagcat attgatgctt aatttgatcg
     9061 atcatcacat taggtaaata aaaagaatag gaaatacgaa aataggcagg gaccaagtga
     9121 taaaagacat tcatcaataa ttcacgaaac gaagcaaatt ctacggccat caggaattcc
     9181 atgcgatgga tgacttctga agaacattcc agcaaaaaat caagtgcagg ctcctggaca
     9241 tctccttcaa cgatcgatgc taagcaaaga gagaaaaata taatctccga attttgatgt
     9301 tcggaatagc caaggccagt caatacttgt cgactaatgt cagtgactgc aagagaagcc
     9361 aacagttcag catcttttac cagttccatg gatgtttttc caattcgaat ggttgtcaaa
     9421 ctcaaataat aagctaattc atccattcga tttggtgcga atcgaagatc tgatagtgaa
     9481 atcgttttat ctagcaaatc tctaactttg gcgtaataat caatggaaag ggttgcggtt
     9541 aaatacgtga gcccaaatct tccagcaagt gactgcatca aacgagcagt gcgatttttt
     9601 gcttctcttc ttaatattgc ttctgaccct agcagcctaa aaccagtttt cctagtatac
     9661 gacagctgac aatcttccac ttctaattca tttcttatct ttttgatatc tgacaggact
     9721 gtccctttag aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc
     9781 gaataaacca tcaaataaag aagatctctt cgctcttgtt cggtatacac agcttcaaat
     9841 cgagtaatat ccaaaaattt ttctgcccag tcgattgctc ggaatttctt tatacggata
     9901 cgggtatcat ccacatattc ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt
     9961 acttcgatga tctctgctgc tttttctaga ctgatttctc ttggtgtttt caggagataa
    10021 ctaattttgt agttcacagc gctgttcacc tcctacttat attctagtat ggtagcgctt
    10081 ttaaatcgag gggtgtttgg tacaacttac agttggattt tttttacgac tgtaccgaac
    10141 gatttctatc gaatggatac agaaaaaaag caggtgacca aatgtctcct gcttttatga
    10201 attgatttcg tccgatcaat gatgatcatg tttatttttt gaggtcagtt cttcaaattt
    10261 ttctttgaca tcttcaaccg ctttttcgat atctttttca aagtgattgg gctcattagg
    10321 ctttgtttcc tcttctacag aatttgaacc agataaagct gtttctttat tttctgtttc
    10381 accagagttt gtgccagcta atgattcttc tttgtcttct gtttcaccag aattagttcc
    10441 tgctaaagcc acttcttctt ctgttttagg ggccattgaa cctcttaatc gtttgtgttt
    10501 ttcaccgtca taatttgtca tgttggttcc tcctagtagt tctatacttt ttcatcgtac
    10561 aataagacag tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt catatttttc
    10621 cttctgattc ttgaatgctt ttgattcctt cttccaaaat tttcatttgt aagattgttt
    10681 cttcatcttt gatgactttt tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga
    10741 cacggctgta atcaccaacc tcggaaatta ctttttcttc atgataactc ccttgttcat
    10801 ctatataggt caatacacca taatgttcgg gcaaatcgat accaaagtct tcatgatcag
    10861 gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc
    10921 ctttttttcc gtaaacgacg aaacttggac gttctttgat gcggaaataa ctggatttta
    10981 tcgaaatttt catatttcca tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt
    11041 tccctaaaag ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa taagaaagaa
    11101 cttgatctac tgtatgacaa gcatgtccat ataaatagct gttcgcagta gaaaattcat
    11161 gcgtattttc tggtacttcc ggtctgaaat aatcataatg catttctact tctaaaatat
    11221 cccctaattt tccgctttcg attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc
    11281 tgttttggta acattggata aatagacctt tttcttttgc taatgcaaat aactcttctg
    11341 cttcctttga cgtttctgca aaaggtttct ctactagtac attcttacca tgttcgagtg
    11401 ccatttttcc atattcataa tgaaattgac taggtgtagt gatgacaact aattggatct
    11461 caggatcatc ccaaagatca gacagctgat cagtatattc gatgtcttcg aatgttttcc
    11521 acactggttc agatggggaa aaaatttttt tgatattgat tttgttcgta agttttcttg
    11581 aaaaaggcaa atgatagcga tttgtactct ttccattgcc aagataagcg attgtaagca
    11641 tgagtggtgc ctccttttat tgatgttttc agtatatcct agaaaagcgc agaaagtatt
    11701 tataatagct attctggtaa aaaagttcag aaaaacactt gttatcattc ggtttggtga
    11761 aattaatgtt caaagtaagg ataaaaatca aaaagatagt agcggaacca agtgtttatc
    11821 agtaagatag aatttggagg tcagatcatg cttattgaag aaaaactaac aaaacaagag
    11881 ttgtttacca cgacagagaa acggattgtc gattacatcc gtaggaatat cgaagctgca
    11941 gtctacatga cgattgaaga attagcaaaa gctacgtata cttcccattc agctatcatt
    12001 cgtttatgca aaaaaatggg attcagcgga tttaaagaat tccgtttcga gctggggcgg
    12061 gaagttcatc aattgattgc tcagttcaat caaacgattg atcctaattt tcctttttca
    12121 agtgaagata gcactcagca aattgcaaag aaaatggcgg atttatcgat ccagtcaata
    12181 aaaaaagcac aattgcagat tgaacatgaa gatttagatc aaatcggtaa agtattgaac
    12241 aaggcacagc gaatctttct ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc
    12301 cagaataaaa tggtcaagtt gaataagttt ttgatcatgg ctgaagaata cagtgattct
    12361 tcttggaatg cagccaattt gactagcgag gattgcgcca tttttattag ctacagcggt
    12421 cgcatccatc attatgaacg aataatgacc tatttgaagc atgtagaggc ccctaccttg
    12481 ctgattaccg gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg tcttgttata
    12541 tcgcaggagg aatatgattt tgctaaagta gcgactttct cctctcaaat tgccttcgat
    12601 tacgtattaa atacgttatt ctcagtgata tattctcaaa attttgaaga gaatatccgc
    12661 aacttgaaaa acaaacaaga attgatgcaa aaaggccttc ttaaagaata tcggaaaact
    12721 taagaaaaga tcctcattta gtaataagaa agatagtgtt tgataggctc tctttgatat
    12781 catcataaaa acaggctgaa gttttctttt tcaaacctca gcctgtttgt ttacggaacg
    12841 attcgaatag atatattata gaaatgagga atagctaact tctgtttctt attttgaacg
    12901 attgacgaaa aagatatata agaaggagga aaatcagtat acatgcagct aacaatccat
    12961 acaggaaaaa tcgtttgttc tcggatactt tctgtacttc tttttgaact tcttttgcat
    13021 gataaggaac ccggtgccct ctgacaagta agcgatgact gttgaccata taaggtgtac
    13081 aagttaggag tgtcacgaaa tcctgccctt tttcgattct cagatctttt gtatcagtcg
    13141 gttctaccgt cttgatctga tcgacttgat aagcgagtgt ccgtccattg atttcgatat
    13201 agaaagaatc acctaaagtc agtttcggta agtctgtgaa caactttgct tgaggcagtc
    13261 ctcgatggct ggaaataacg gcatgtgtat tttctccccc cgtaggataa gacgttcctt
    13321 ccaataatga ggcacctttt tctagcagca cagtattcgt ttcatcaaaa attggcaaat
    13381 ggacattgat ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa taggttctat
    13441 cctttttcgc aggttctttt ttcttagtaa agggatctgc acctggattt ccaccttctt
    13501 ttgctaattg ctggtttttc tttgtcatgt tttcttggat tttagccatg acttcttcat
    13561 tttctttgac cgcttgttgc tgataatgag agatgatctg ctgatccaga taattattca
    13621 gcgcgtcgct aacaaatgga taagctagta tccctattcc tgagagaagc aagaaaagca
    13681 tcagcagatc tagaagtttc cttttctttg gtttcatttg tgttctcctt gtgatgactg
    13741 tttgataata aaagaaaaag gagcatttgc gtaatccgct gctccttttt ccaattcttg
    13801 caaaaagact aaatctgact gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga
    13861 aggactactc ctgcaccgat atacacatag attcccttac cgcctgttga aggaagtgta
    13921 cctttgtgtt tatttggtat tttttcagga gaaatcaact gtcctgctgt tacataagat
    13981 tgttcatcga tagtaaatgc tacacggttt gttaatggaa catatttttc tggcgctttc
    14041 gtttcttcca gatagtacgt gccatatttt agacctgtca catcgattaa accgttactt
    14101 gtggttgtaa aaaccgttgc tgattctttc gccgataccc agctgacggc ttttgtggat
    14161 gggtcgatcg ataaatattt cgctgtgtca ctatcttgat cacgaacgac gaattctgct
    14221 ccagcaagtg tttggtctga tgtaacgtca ccatctactt taacaaatcg tttgccccca
    14281 gtaacgacat cgactgacgg tggtgtttga tcatttgtat gcccgttatc gacatttgct
    14341 tggttagaaa atcctttggt tggatctgct ttttcgttca aatgcatata gtaaacgaat
    14401 ttcaatgtac cgccaggagt taatgaagga atataattcg gatcaactga aaccgtgaat
    14461 ccgtctgttt gctcagtgac agaataattt actgggtcga tttctttatt tccatcatat
    14521 aaggcataag catacgttcc tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta
    14581 tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc gatacctaat
    14641 gggatattga cagaaatctc atattgaatt ttttcaccga ttgcgacatc ttttccattc
    14701 aattgaggtg ttgttttatc aactttagac gtatcatttt tgatggtctt ttctactggt
    14761 gtttggctat ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat catttccgca
    14821 ttatcaggag cttttacttc ttctaaatta tattttccaa cttctaaatg attaacgatc
    14881 aactggcctt tttcaataga tgcctcggca aagtcattgt tgccgatgtc ataagaatga
    14941 ccagtaatga aatgtttggc tttggtttga tcagttgtcc aagtgtacaa tccatctgtg
    15001 acactttgga tgtatttttt gacgcttggt gttccttctt ctttagaaat aataaattct
    15061 gctccattta gtgcttcgtt ttcggcagta ccgatttttg taactttcaa cgttccatca
    15121 ttaccgactg tatttttagg gtagagatgg atagtatcta gttcttctgt cccgtattta
    15181 taagagccat ctgcttgttt gatcatctca tatacaggga aagctaaaac catgtttgcg
    15241 gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt agactgcatc tttcccattt
    15301 tgttttttag gtaaagataa agtgacattt ccatcagcat ctgtcgttcc tgaagcaact
    15361 ggtgtaccag gagtcaaaga ctggactgct tgttttgctg catccacgga cgctccttta
    15421 tctcgttgcg cataaaattc ttgagtgacg ttataaactg aaaatgaaat atcggctaat
    15481 ccttggtatt gatcgaattc gctcatttct ttcccgctgt tttggattaa aggatcgggt
    15541 aaatcagtca ttttcttttt atgaaggatg acttgagctg ctgtctcctc tgcaaacact
    15601 ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata
    15661 acgtttattt ttttatgatt tttcattttt tcactcctgt tctttttttg ataaacaacc
    15721 agattgcaaa gcttgcgaat accataccaa caacgcctaa aagtgtattt ttcgtttcgt
    15781 ttgtttttgg aagaaatgga cggctatttt cttttttatt gactactttc aatatactgt
    15841 ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa cggaatttct gtttcactta
    15901 aaatataacc agaaggtgct tcgatctctt ttaatcgata acttcctgtt ttcaacccgg
    15961 aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg
    16021 tccacttgta tccgttggct gtttgctgga ggtaatttcc tgctttgttt ttaataagga
    16081 atactgcgcc agctaatcgt ttatctggtt tatttgtctc gattttgaca aattgcttac
    16141 tccctgtata tacagagact ttctttgtac tgacaccaaa accaaaatcc agtgttccgt
    16201 tgttgttgat ttccttgttc gcctgtgctg tactgcttag ctgcatctga taagaaatgg
    16261 tcagtttttt tccagcatat ttttccaacg aagggatcga aaataaaaca tcaaatccgt
    16321 gaggtttctt gtgtatgaca aagccttctg tcagcttttc tccattcgat gaaatcgtta
    16381 aactattagg taaaaacgtt aatgcttcat ccgcactatc tgacaattcg aacttttgat
    16441 agtcaaggat atttacagga attgtcgtat gcaaggaata agtgattttt tcaccaatcg
    16501 taaaatcatt tctaggctct tctaatacct tctcaaaagg tggatcaggg tagtcgtttt
    16561 cttcattttt aggatacaaa tggatagttg aaagtttttg attgttttgt ccatgtacag
    16621 gaagaacaac taccatattc tctgcctttt ctttgacgac ttctggtact ttggattcaa
    16681 tgaaaagata cgctttatct cttttttctt gatctttagc ggccagagaa aaagaagcga
    16741 taccatcttc gttgttaaga gttgtagttg ttgcttctgc ggtaaacata ccggaagacg
    16801 caccgttttt tgcgatctct gcttgtgctt cttctaccgt tttgcccttt tcccgtagat
    16861 ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac accatttaat cctcgatacg
    16921 tttgtaataa agctttttct tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg
    16981 ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct gttgctacct
    17041 gagtaaaagc tggtttaaat agtaacaaga agagacacat actaagccaa ccaagttttt
    17101 tcatatttag ctgccccctc tttctttttt caaaaaaaga taccaaatag aaaacgcaca
    17161 accaatcatc cctactagat agattcctaa acggccaatt cctcccgttt caggtaatgg
    17221 tacttttgcc tgattcgtga tgtctaaaga aatctggttg tttttggctc ctggtgacag
    17281 aacagattca tgatcttgtc catccacttg aattgaccca tcttcgtgta taactatagt
    17341 aactggctct tttagacctt ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt
    17401 tccaggtttt aaattttcga atagaaaggt atctgtttct tgtccatctt ttggcgattc
    17461 taagtccatt tctggtccct gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc
    17521 ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca
    17581 tacattattt gtcatctcgg tattttttcc cgtgtaaatg atttttccat atttatcgat
    17641 ttggaattca taatttcccg gcgaagtatc atatccggca ggaccagctg tttcaacgac
    17701 tcgatattta ccaggtgtga gtgaatcaaa actggcaaga cctttttcat tcgttgtttg
    17761 gctgtcaatt ggctgataag taccatttgc ttcttttttc tgtagggaaa atgctgctcc
    17821 tttaagattt atctctttgt ccgtcccttg catcgcatac ttacgtactg ctacaggaac
    17881 ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca tcggaagtag tgactgtttt
    17941 tccatcaatg gttaccgtac catcagaagc gatcttgatt tcccaagtag tctttttgct
    18001 taatccatgc ccttctggag cttttgtttc tgtcagcgta taggtcattt ctttttgcaa
    18061 tttgacattt tctggaagag aataggttcc gtcgccatga tctgttagta aagtatcaat
    18121 agaatctcct gttaatttga aaacagcgcc aataagatcc ttttcacctg tagaggaatt
    18181 tttcgttatt ttcaagttta acggtgtgaa ttgtttagta tttttccatg tcattgcatc
    18241 tatttgttga gaatcgtatc ctggtacagg taattctttg attgtttggt aactgaatgc
    18301 ttgaccttga ttattgtatt gaggtagtga taatatctct tgataacttt ctccgctatt
    18361 tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt tagctggttt
    18421 aatgattcga atatatccgt ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat
    18481 ctcaaaagta acttgatctg gacgatcagc tagattgttg tcaaattctt cccataactt
    18541 tttgatgtga agactgactc cgggagcttt agcagatggt ataccgaatt cagctaattc
    18601 attggtatca atgtttggct ggaaagttgt cctgccgttc atttgatacc agaaatttgg
    18661 atggaagtcc tcattttctg tttggattct cacttggtaa tggatttgga tttcttggtc
    18721 ttttcctaaa tagatctgat tgctcttgat ggtatttcct tctatggaaa tagatggagt
    18781 gaccgttgta ggacttgtcc ccacactctt gactgaaagt gtaccaggct gataaatgaa
    18841 tggttctgcg attggatcat ttatttgtcc attgcttaca gttgctgaga tctgtacagc
    18901 ttttttggct aggtattcag agatatctgt tgcatgatca gctgattcat agtaaagatc
    18961 gcctttttca tctgatgaaa ccatttgtcg catacgagac tctacttctg cttttgagag
    19021 accagctgcc ggatcgcttt gaagttggat gccaagacca tgtatttcga ttcctcgttc
    19081 tttgagtgcc atcgcttctc cgatggttgc gataaacgta ctgtcgattc ttctggatag
    19141 attgttttgg tcaggtgcat catagattct tgagattaga gaagtatttc ccggccgatc
    19201 ttgcgtatta gaaaactgag ttccgtaata attgctgctt gattgtgcgt gtacccgctg
    19261 tactttatag gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc
    19321 atttggaacg gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc
    19381 atttgtccgt gaaggtgtaa tggattttac ttgatttttc actgaatcaa atgaacccat
    19441 ctgtactgca ccgttactgt agctatatcc ttcgcttgag tagccgacat atcccatatt
    19501 gattttgtct gtgataccgc tatctgctaa agtatcgaca aaacgatcga caccaatctt
    19561 tacttcaccg atccgattat tgtcgttcat acttcctgac cagtctacga ccaatacgag
    19621 atcaagaggc gtgatatctt tttgtacatt tccacgagca ttcaaataaa catcaaacaa
    19681 tccgggtgta cttgtttcct ttgcatactt tcgtatcgca tagtctgctt gattaccggt
    19741 tcctccatat tcgatatacg aatgggtccg atcattagga tctccattcc aagaatttat
    19801 accatcccat tgatttgttc cgtttttatt gccttgatga tcaagaacat ttgtatttcc
    19861 tgtaggttgc caactggctt taggataagt gccctgatca tctgttgtat attctgtagg
    19921 aatcgtttga ttagatggca cactcacagc tggcaaggat aatgtaggca atgtaaatgg
    19981 gctgacagaa ttttcgtttt cttttgatga ttcttcttct atttcgtctg gctttggtac
    20041 tgacacatct gtatctttct tattttctgt ctcatcaata atgtcatatt ctttattatc
    20101 gacgatcagc ttgtttgcct ggctgtggta aagtgctgga tcgattggaa aactcagatc
    20161 gatagtttgt ttagtgtttg atgagatttc caacgtaatt ttcccgctat ttacctgata
    20221 agcatagcca tctccttctt ttctttccac atcacttaag taattttcga ctagaccaat
    20281 ttctttttct atcttttcag cactggtgtt attcgcattt agcttgatgt tcagtgcttt
    20341 ttttccagac tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg ttggatcatc
    20401 tgtagtgtct gctattgcag ataatggcac aaagtttgat aaactcaata ttatcagcat
    20461 gaaaataact ttcagtttct tccctgtggt cgtcattttt cccctccttt ttcttttttt
    20521 ctgttttgac gtgaatagtg aaaattatta aaataaacag gcgttacata attttattta
    20581 ttttaatatg ataattatta tacgaagggc gctaaaggga atcacaccaa ttttcatacc
    20641 aattggtttc atttatgaag ttgattcctt cgattaatga gcgtttcatt gaaagaaaag
    20701 ggttgaggga gaaaaacata tgatcaggtt gttggaagga aactatagaa agatggaaaa
    20761 aatcattcga attctgtcca tttctgacaa gtggatgact aaaaaagaac ttgccaaacg
    20821 aattggtagt tcagaatcta catttgtaag gtatatcgaa gagatcaaaa tccgctgggg
    20881 aacggatgta acgattcaat cttcacaaaa attggggtat cgtcttgaac atttgaatgt
    20941 accagcttat ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt
    21001 aaatgagatt attgtcaatc cgggtaaaac gattgagtat tattgtgaaa aaatttccat
    21061 cagccggtct tcttttacac gtaagttgaa acaatgcaat caaattttag aaagctattt
    21121 attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt
    21181 gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc
    21241 aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt
    21301 tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat
    21361 gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat
    21421 aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag
    21481 cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa
    21541 agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc
    21601 cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt
    21661 tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc
    21721 acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt
    21781 ttattccact ctactgaatc agaatctgat ttattattta tctagctata tcttttggat
    21841 gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag
    21901 cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt
    21961 gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct
    22021 tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat
    22081 attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt
    22141 atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac
    22201 acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt
    22261 cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta
    22321 cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat
    22381 gattgattct taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc
    22441 tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga
    22501 tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat
    22561 gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt
    22621 tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg
    22681 acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa
    22741 atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag
    22801 tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag cccttcattg
    22861 tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga
    22921 taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt
    22981 tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc
    23041 aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca
    23101 attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac
    23161 tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta
    23221 acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt
    23281 tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca
    23341 gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa
    23401 atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct
    23461 tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat
    23521 gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg
    23581 taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa
    23641 tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc
    23701 ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc
    23761 caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg
    23821 ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga
    23881 ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg
    23941 cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg
    24001 atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt
    24061 cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg
    24121 catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag
    24181 gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat
    24241 tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta
    24301 ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga
    24361 ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc
    24421 ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt
    24481 gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg
    24541 acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta
    24601 atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga
    24661 ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact
    24721 tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat
    24781 gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca
    24841 aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc
    24901 aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt
    24961 gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg
    25021 ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca
    25081 gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt
    25141 gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca
    25201 ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt
    25261 tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa
    25321 gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag
    25381 atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat
    25441 atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta
    25501 cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa
    25561 gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt
    25621 gggattcatt actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt
    25681 agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg
    25741 cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt
    25801 aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga
    25861 gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga
    25921 tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc
    25981 cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat
    26041 ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat
    26101 gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt acttgtctag
    26161 tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc
    26221 ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac
    26281 caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac
    26341 gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg attctgatca
    26401 gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc
    26461 atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt
    26521 agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc
    26581 attaaaatat acgaatccag aagataagat cattttggat tcatctgtta accataaaga
    26641 atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat
    26701 ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct
    26761 tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa
    26821 ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg
    26881 cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata
    26941 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact
    27001 tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca
    27061 tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca
    27121 tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca
    27181 tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt
    27241 ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc
    27301 ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt
    27361 gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct
    27421 agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca
    27481 ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag
    27541 cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc
    27601 attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc
    27661 tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga
    27721 tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta
    27781 tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt
    27841 tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta
    27901 taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat
    27961 gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt
    28021 cctcctagca aatagattta tgattggtat
//
LOCUS       DARBER010000022        26373 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARBER010000022 DARBER010000000
VERSION     DARBER010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.022"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            369..557
                     /locus_tag="SCK06_000977"
     CDS             369..557
                     /locus_tag="SCK06_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150998.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            547..807
                     /locus_tag="SCK06_000978"
     CDS             547..807
                     /locus_tag="SCK06_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1150999.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            848..1075
                     /locus_tag="SCK06_000979"
     CDS             848..1075
                     /locus_tag="SCK06_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151000.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1140..1712
                     /locus_tag="SCK06_000980"
     CDS             1140..1712
                     /locus_tag="SCK06_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151001.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1713..2024
                     /locus_tag="SCK06_000981"
     CDS             1713..2024
                     /locus_tag="SCK06_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1151002.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2015..2275
                     /locus_tag="SCK06_000982"
     CDS             2015..2275
                     /locus_tag="SCK06_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151003.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2436..2795
                     /locus_tag="SCK06_000983"
     CDS             2436..2795
                     /locus_tag="SCK06_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151004.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2792..4435
                     /locus_tag="SCK06_000984"
     CDS             2792..4435
                     /locus_tag="SCK06_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1151005.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4470..5552
                     /locus_tag="SCK06_000985"
     CDS             4470..5552
                     /locus_tag="SCK06_000985"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151006.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5596..6768
                     /locus_tag="SCK06_000986"
     CDS             5596..6768
                     /locus_tag="SCK06_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1151007.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6755..7459
                     /locus_tag="SCK06_000987"
     CDS             6755..7459
                     /locus_tag="SCK06_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1151008.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7464..8624
                     /locus_tag="SCK06_000988"
     CDS             7464..8624
                     /locus_tag="SCK06_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1151009.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8700..8978
                     /locus_tag="SCK06_000989"
     CDS             8700..8978
                     /locus_tag="SCK06_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1151010.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8959..9330
                     /locus_tag="SCK06_000990"
     CDS             8959..9330
                     /locus_tag="SCK06_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG1151011.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9346..9750
                     /locus_tag="SCK06_000991"
     CDS             9346..9750
                     /locus_tag="SCK06_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151012.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9747..10157
                     /locus_tag="SCK06_000992"
     CDS             9747..10157
                     /locus_tag="SCK06_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151013.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10218..10805
                     /locus_tag="SCK06_000993"
     CDS             10218..10805
                     /locus_tag="SCK06_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1151014.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10878..11345
                     /locus_tag="SCK06_000994"
     CDS             10878..11345
                     /locus_tag="SCK06_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG1151015.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11391..11699
                     /locus_tag="SCK06_000995"
     CDS             11391..11699
                     /locus_tag="SCK06_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151016.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11894..18628
                     /locus_tag="SCK06_000996"
     CDS             11894..18628
                     /locus_tag="SCK06_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1151017.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18632..19504
                     /locus_tag="SCK06_000997"
     CDS             18632..19504
                     /locus_tag="SCK06_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG1151018.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19501..20550
                     /locus_tag="SCK06_000998"
     CDS             19501..20550
                     /locus_tag="SCK06_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151019.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20554..21378
                     /locus_tag="SCK06_000999"
     CDS             20554..21378
                     /locus_tag="SCK06_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151020.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21396..23438
                     /locus_tag="SCK06_001000"
     CDS             21396..23438
                     /locus_tag="SCK06_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG1151021.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSIDFGGYD
                     YSGKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTY
                     TLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVN
                     YQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLG
                     ENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNF
                     GELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQIDWLKIEKGDTRTP
                     NISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSIEGLKNFEDGIQS
                     KGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGNDSTILT
                     IPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            23452..23601
                     /locus_tag="SCK06_001001"
     CDS             23452..23601
                     /locus_tag="SCK06_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151022.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            23598..24044
                     /locus_tag="SCK06_001002"
     CDS             23598..24044
                     /locus_tag="SCK06_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151023.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            24046..24183
                     /locus_tag="SCK06_001003"
     CDS             24046..24183
                     /locus_tag="SCK06_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1151024.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            24220..24513
                     /locus_tag="SCK06_001004"
     CDS             24220..24513
                     /locus_tag="SCK06_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151025.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            24510..24734
                     /locus_tag="SCK06_001005"
     CDS             24510..24734
                     /locus_tag="SCK06_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG1151026.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            24731..25750
                     /locus_tag="SCK06_001006"
     CDS             24731..25750
                     /locus_tag="SCK06_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG1151027.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            25939..26012
                     /locus_tag="SCK06_001007"
     tRNA            25939..26012
                     /locus_tag="SCK06_001007"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25973..25975,aa:Ile,seq:cat)
BASE COUNT         9379 a         4047 c         5563 g         7384 t
ORIGIN      
        1 tccttctaaa ttgttcttat tatctatcat cagttgctgt ctattgtcat tatgtcactg
       61 tggtggaaag gtgtatcgct catctaaaat ttaggtgcaa actgcaaggt tcgattcctt
      121 gccagtgact ttggtaaccg aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg
      181 tacgcgtttt gtgcatcttg taggttgcta ttacataatt ggttgggtta gattgagttt
      241 tgggattcgg tacaaatgaa tcgtcaaatg actcaagcac aggatcggaa acgtccctgc
      301 ctgtgcatta catattagat cactctttga gtggtctttt tattttaaaa aagagaggat
      361 tttgaaaaat ggaattgatt gtatttacta ataacggaca aacatatcat ttttatgaag
      421 tggagaattt taagccaaca acaacaggat ttagttttat ttatactggg aaggctacgg
      481 gagttactcg ttcagctacg tttaacaata catcaacagc tggttatgcg cttgcggagg
      541 tacaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg tgaaagaatt
      601 gaaaatgaga ttgctgacct aagtggagca gtgcctgagt ttagtatttt catagtctgg
      661 tcatgcaaaa cactgcaaaa tagcaaagca ttagtcagcg ctagcttaaa aggagcaccg
      721 tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac ttatctcaaa
      781 aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac ataaaggagg
      841 ctgcataatg agaaactact ggtatgtatc actaactaat gaatatcctc gaaccattgg
      901 tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact ctattgtcga
      961 aatgctaaga gaagctacac caaacgaagt ggataaatgc aagctgatat attgcggtca
     1021 tggctattgg aaagacgagt atatccaata caacattgag aggtacatca aatgaatgat
     1081 aactatgatt acatcaagtt gttagcaagt aattattaga tgtttttagg aggaaaacta
     1141 tgaaattaaa agattatatc agagaagggt ataacgttgt aactgcaccc aatctagctt
     1201 acaaaattca agaagattat cccaatgctt tagtgtttac tgatagagct ttagatgcca
     1261 ttcctatagg gaagttatta gtagatcgtt tttatagcaa taatccagct gttcttagtg
     1321 ctaagccact tcaaaacgct tatgctattg aacgctttac ttgcgagttt actggatatg
     1381 agacggtagt accaatgaca ggtgagacag gaagaaaagt aattcaacag atcaaaaaga
     1441 ggattgctga attgacgtta gatgatggaa gtaatacgaa ccttctagaa atcaaactaa
     1501 gagatactga ctcagtacca gaagtttatt ataaaggtca acgaatggat gagatgccga
     1561 taggtcttgt cgatattact tatcattggg atacggaaga atatgaatgc caagtaccaa
     1621 aaggagctaa cgacatcact attgaatatt atgattcaca taataacaag tacttagata
     1681 gaaaaatcat tgggcacaag agagatatgt aaatgattga tgtttcaagt aaacaagcaa
     1741 gagctaggtt ctatggatca actgagtgga ggaaacttag aagtcaatgc ttagagcgag
     1801 atcattatga gtgccaatgg tgtaaagcag agggtagact aacaactcaa catgattcaa
     1861 ttcttgaagt ggatcatata aaggagctgg agtatcatcc ggatttagct ctagatataa
     1921 acaacctaag aaccttatgc aaagattgcc ataacaaaag gcacaaccgt atgaaatatc
     1981 gtggccaaca aaagaaaaga aagtgggatg atgaatggtg gtaagaaaaa cagcaaacag
     2041 agatgatgtc aaacgagcag aagcaatcgc tatggttgat aaggctattg ataagttggt
     2101 tctatttaag agtcagctca ttgaaggaac aacaactgta gaggatatat ctatcaacaa
     2161 tagactaata ggtaacgcta atgaagtaac agggacattt aatattaatg tagacattga
     2221 ttataccttg agtccatttg cacatattgt agaaagcaac gagcaagacg gctgataaat
     2281 cagtataaag cgaagatata tagagtgaaa tacccccggt caaaagattt tgagaagaaa
     2341 taaaattttt gggaaccggt ggatggggtc aactctgcaa ataaatgatg ctattttcac
     2401 acgaccccct ccccacgtag aaaggaagtg agttgatggc tgatctgcaa gagagaaata
     2461 ggcttgtttc tgctgaagaa aggcgtctaa ataaattgtt taaggatatt gataaggata
     2521 aaaagaaggt cgtttctggt ttgattacgc aagcggcacg cttaaaaatt cttcttgatg
     2581 aaatgtggat tgatatttcc gaaaaaggtg attatgagct tttctctcag tcagaaaatc
     2641 aaaaacctta cgagcgggaa cgaccagttg ctaaacagta taattcgaga gaccaagcat
     2701 atcaacgtgt aattaagcaa cttactgact atttgccaga agaaaagcgc gaacctacaa
     2761 aaaatgcagc gctagatggt agtgatttgc tgtgacaaca ttaaagccat tctattttga
     2821 tgaatacgta gatttatacg aaaaaggggt aatccctttt aataaagaaa gaattcagtt
     2881 aattacttat ttaaaaaacg aagtgctagt tcgggatgat atctattttg atgaagaaat
     2941 gattcaaaag tttatcaagt acactgaaaa aaattttttt cctttagcta aatatcaaaa
     3001 atttatcaca cctttcattt tctgctatca aaaagaggac gacgaggtgt tttttgatga
     3061 aattctaaat agtattgcgc gaggcggcgg gaaaaatggg ttcatgtcag caagagactc
     3121 attttttatt agccctctct acggtgtcag aaattatgat gtaacgatta cagcgaattc
     3181 tgagaagcag ggaaaggtat cttttaaaga agtttatgaa atggtacaaa tgcgcaaact
     3241 ggaaaaacaa ttttatttga ctaagatggc tatagtcaac aatgtaacta attcgatttt
     3301 tagttatagg acaaacaatc ctaagactat ggatagtgca agagatggct gcttagaatt
     3361 tgatgaaatc catcagtttg aatcttcaga cctagttgat attcagcgta gtgggctagg
     3421 gaaaataaaa catcctcgaa ctttttataa tggtacaaat ggtcatgtta gagaaggttt
     3481 ttatgacaaa atgttggaac gagcacaaaa gatttttagt ggagagaaca agaatgatcg
     3541 cttgtttccc tttatttgta aactggataa cattgatgaa atggataatc ctaaaatgtg
     3601 gtcaaaggct aatccaatgt tcgaagaaga ttcagcatat tcaaaacgtc tgttttctac
     3661 agtgatgaaa gaatataaaa aactagaaga tgagccatca ggtaggcgtg aatttgttgt
     3721 taaacgaatg aattttacag aaggtgatgc agaatcagat attacgactc atgaaaaatt
     3781 aatggctact aaacaagaat tgccaaattt gaatggtcgt tcttgtgtgg ctggatttga
     3841 ttatgcaagt attcgtgatt ttgcaacagt gggtttgctt tttaaaattg atgataaatt
     3901 tatctggcag caacatagtt ttgctaggaa aaagtttcta gatacattca aactgaaagc
     3961 gcctattaag gattgggaga aaaaaggttt agtgacaatc gttgatgagc catcaattga
     4021 tcctcgtcat ttaattgatt ggttaaatga aaaaagaaaa atccatcaaa tagaaatcgt
     4081 ttgtgccgac ggatttcgta tggatttgct gaaacctctt ctagagaaag aatcatataa
     4141 ttttgagttt atcagaaata taagaggtgt gcagtcaaaa gttgctccta ttattgaaga
     4201 tggttttgca aatgaacggt ttatttttgg tgatgatcct atgatgcgtt ggtatacaaa
     4261 taattcgttt atcaaagaag acaaattagg gaataaaaca tttttgaaaa aagagccagt
     4321 aaggcgtaaa acagatgggt ttcatgcttt tttggcagct ttatataaac gagaagaaat
     4381 aactgatatg gattatgagg aagcatttga tatgttggat gagattgaat tttaaggtat
     4441 actccttatt gaaaggggta gttatagaag tgaaaaagag atattttcta tttgttacaa
     4501 tatttataat aatagcaatt tgtgttttga cagtattttt accacaaatt actgagtatt
     4561 taaattatcg gatatttacc acaaaagtag atgatagcgt tactagttta agtaggctag
     4621 aatttataaa aatatgtgca tctgtgtttg gtcctatttt gactgtaatg gtatttgtga
     4681 atactttaaa tatgcagaaa aaaacagagc aaaagcaaaa tgagcttgaa gtaaagcaag
     4741 ctaaggctga gaacgagaga gcaaaaaaag agctgttaac acagatagat cgtgagttct
     4801 attttttatt aaatatgttt attgaaatac aaaaagatca aaatgccaaa caagctgtta
     4861 ttgctttaca gaagttggca atttttaacg aaaagggttt taatcaagac tatgcattta
     4921 cttctaaaga tttatcagat agtgaaattg ggagtacaat tgaacaatca ggaccgctga
     4981 agggcacagt tgaatatata aaatttgaaa catttgatgt aagcaagtcg atggggaatt
     5041 atcaaacaaa aacaaaagac gttgctacaa gtttacacga tcagaaagta gctatattga
     5101 atgaacaata tgaaaagatg caaatatatc ttggacggta cttcaaaatg tttcaccgaa
     5161 tagttaaaac attaaatgag tattatgacg attataatga ttttgatgtg aagaggtata
     5221 caaaatatat agggacatta agaacacaaa tttctccagc tgaattccaa gtgattttat
     5281 tcaattctct atatataaaa cgaggttttg gcttaggaat tcaacttatt ggaagtggtt
     5341 tttttggaga tgattttgat tttgaaacta atcaacattt tgaaacaagt atcaatgaac
     5401 agtggtttct atcactatca actgttgaca gtgataactc tataaagcga caaaaattat
     5461 ctgaatatat taaagaatta ggatccgtag aatataaaaa aatagctaat tttgaaagtt
     5521 tatataaact ctttaatgaa actaagctct agaaataggg cttttttatt tttctttgaa
     5581 aggtggtgaa aacatgtgag cttatttgat atctttaagc agtcaataag aaatgaagag
     5641 ccttctgagt ggataccgga ttttgtatat ggagaggaag tatctgctcg ggcatacctc
     5701 aaaattatgg caaaaaatac tgttttagat tttgttgcaa gaactatgtc aacgttagaa
     5761 gttaagttta aaaataaaga tgggacatca gactgggatt atattctaaa tgttcgacct
     5821 aattctgata tgtcagcagc tacattttgg caaaaatttt tttatcggct cttagatgac
     5881 aacgaagtgt tagtaatatt tacagatgat aatcaattgc tgattgctga tgattttagt
     5941 cgaacggagt atgccgtgta tgatgatgtt tttacaaatg tgatggtaaa aaattttgtc
     6001 tttgaacgtt catggaacat gtctgacgtc atttatatgg aatataataa cgaagaatta
     6061 gaaaggttta cacgaggttt atttgaagat tacgccgcgt tgttcggacg gattctagaa
     6121 gttgcaatgc gtaacaacca aattcgtggg tctgtatcca ttgattcaac gggaacggcg
     6181 aatgatgaaa aaggaaaaga tggtaagact agatctcaaa gattacaaga atatattgat
     6241 aggatttgga actcttttaa aactaactca gttgcaattg ttcccaaagt gaaaggcttt
     6301 gagtatgagg aatacaccaa taaacaaggc gtatcgaatc aatcgctaga tgaactgaat
     6361 aagatgaagt cgtctttaat tgatgatgtt gccaacgcca taggagtacc tacggcgctt
     6421 atttatggtg aaaaagctga actggattca aatattaaag cttttcgtaa attgtgtatt
     6481 gctccactag ttaaaaaact tcaagacgaa ttggtggcaa aaataattag tcgtcaagag
     6541 tacaaaaacg gggaacgtat agaagttaaa aaagttctcc aaaaagatct attagagtat
     6601 gctactcaaa ttgataaaat aacatctggt ggtgcgtttt atattgacga agttcgtgat
     6661 gaagttgatt atgatgagtt gccaaataat gaaggaaaaa ttcgtattcg tacgaaaaac
     6721 tatgaagaaa ctgtgaaggg aggtgagaac gacaatgccg aaagctaaaa aagtaccatt
     6781 tcagtttact aacgaaattc agaatggtaa gcacattctc accttgagtg gaaatgtaca
     6841 gaaaaaatat tggcgtgatg atgatgtcat aaacgctaaa gatattcgtg actctctaga
     6901 tactgtaacc gatgatattg taatcaagtt gaacagccca ggcggtgatg tgtttgaagg
     6961 gattgagatt tacaattatt taaaagatca cccatcaaat attactgtag aagttacagg
     7021 gcttgccgca agcgcagcaa cgtttattgt cgctggtgcc aatgatgtga ttatgaatgt
     7081 tgggacttct ttgatgattc atgaagcttc tacatttgct tgggggaata aacaggacat
     7141 ccaaaaaacg ttaaacgcat tggaaacgat cgatgattct atcttagcta tttattcaga
     7201 taagactggc caatctgttg ataagttgcg tgaatggatg aatgaagaaa aatggtttac
     7261 agcagatgaa gctgtcaaat atggatttgc caattctgtg aagcgtgaag aaccacaaaa
     7321 tgaacctcaa aatattgcag cgatgattca agatgctgtt gccgttgcaa tagcgaattt
     7381 aaaggaacct gttactaata aaatcgaaca agaatcaaaa caaaaatcat taatcgcacg
     7441 attgcgaaaa ggagaataaa gatatgttaa aaattacaga taaaactgca gatgcaaaaa
     7501 aggcatttaa tgctatttct tcgaaagaag atgcaacacc cgaacaaatt aatgctgctc
     7561 tagaagaata tgtaaccgcc attgcagaag atgccggcaa acaggtgcgt gctgaatatg
     7621 aagaattgaa aaatgtgacg gacaaccgtg tattagaggc tcgtggaatt cctacactaa
     7681 cagcagaaga aacaaagttt tataatgaag ccgtaaaatc tggaggtttc gattctgacg
     7741 tagtttggcc agaaacgatt ttggaacgtg tttttgaaaa tcttcaaaac gatcatccta
     7801 ttttaggcct cattaacttt acaccaactg taggtcgtgt caaagtaatt agagcacgtc
     7861 gtaaaggtgt tgctgttttt ggaccattgc ataaagacct tgaaggccaa ctagatgctg
     7921 aatttggtgc aactgaattt gttcaacttg cgttgaccgc atttttctta atttctaatg
     7981 ataccttaga tttaggacca cgatggattg accgttttat caatctttct ttaagtgaag
     8041 cagtacgtga tgtttgggct aaaaaaatta ttacaggttc tggtaaagat gagccaattg
     8101 gattgcttaa agatctagat ggtgcggtca caagtggtgt atatccagat aaagctagcg
     8161 cagggacatt aacttttgcg aaagagaaga ttgttcttga acttgcaggg gtgatgaaaa
     8221 aattatctaa atacacatac aaagtagatg gtgacgatga aggtgaagtg aaataccgtt
     8281 ctgttgctgg acgagtgtat ctaattgtta atcctgttga ttactgggat atcgtagcta
     8341 gagttacatt tgcaaattta aacaatgtgt atgggactac gatgccattt atcgctgttg
     8401 atcatatcat cgaatctgta gacgtaccag aaagtaaatg tattgcatac gttgataaag
     8461 agtatgaagc tactcaatca agagctgaaa aagtctatgt atataaagaa acttttgcaa
     8521 tgaaacgcgc aactttgtat gctgtcgaca tgcttggtaa tggctatcca acaaataatg
     8581 atgcagcaca agtatatgac ttggacttta cagcggggga gtaatgtccc ctgcaattgg
     8641 tgagataaca ccgacgactg atggggcagt cattaatctt tcgtaaatgg ggttagagta
     8701 tgaacactga agaatttata aaagaatata aggcccgatt tcgtattttc catacttccg
     8761 aagatgagga tatcgggaaa cagttagaga gcgggtttgc cgatataaaa tctctaatcg
     8821 gcagttttga tccttccact tatgagaaag gaaaagaatt agtttatgaa cgtactcggt
     8881 atctacggaa cgaatcacta gagtactttt acgacaattt tcaaacaatg attatggatg
     8941 catcaattga tctggcaggt gctggatatg ccaattaaat caaattataa gaagcctaaa
     9001 attgtagctg gtgatctaaa tacgccagtt actttttttg agtttcaacc taataagggt
     9061 ccagaacctg gtgatgaaga aaaaaaggaa ctgtattttt gtacagcact agtttacaac
     9121 ccatcaatga aagatcgtga cattcttggc acaaaagaag gcgttacgat aaaaatacgg
     9181 gatccccatc aatcttatat tcctactaac aaacataaag taaggatatt agattatcga
     9241 tatggagata aagtttggga aataatcgat gtctcaccgg atttggaaga aaattcaatt
     9301 gtaaagatag tattagaatt aattacttaa atttggtgat aaatgatgag taacgtaaag
     9361 tttgaaggcg ttcaagaaac aatccaagct ttagaaaaaa agtatggaga aaagaaaact
     9421 aagacattaa caaaaaaagc aatcaatgtt ggcgctgaaa aagtagaaaa acagttgcaa
     9481 acggacatga ccgtttttaa agacaaaggt tatacgatcg ctgaggttgt tcgcaagaac
     9541 gctacgtata gagattacaa ggcagaagct gaaatcggtt ggaatggtcc acatcaacga
     9601 tatagaatca ttcatttgaa tgaatgggga tatacacgaa agggcaaaca aattagaccg
     9661 agaggatttg gtgtaataac aaaatctctt aagaattccg agctgttgta ctttgatgct
     9721 gtaggagagg aggttaaaaa gagtttatga aagatatgct taatatcatt tacgaggctc
     9781 tttgtttgaa tgagtatatt cattcaatga cctacaacga gaataccgag caatatcgta
     9841 ttaaatttta tgagcaacct gaaaccgccg ataaaacagg tccaatgatc acaattcgtc
     9901 ctgtagatac acctaacgaa gcgtaccacg gaagtaacaa agagctttct gtggagcata
     9961 tgatacagat tgacgttgaa tcgaaatata gagcaacatg caagcagatt caatatgaga
    10021 taaaaaaaga gatgaaaaaa ctaggctttg gccaaatttc cggtcaaggg ttagatgaat
    10081 actttactga aacaaaccgc tttgtagacg cgcgtcgtta cgaaggaaat acaaaaattt
    10141 acgataccga atattagaaa ttaagacacg aaaataacgt gtcttttttt attacccaaa
    10201 attaggagga aaattttatg actttagtag gattcaaaaa aatgacaatc ggggtctttg
    10261 acagcacagg gaagattcca gcagcaaatt tatatgtaat tgaaggtgaa caaaacaaag
    10321 gggcgactgt atctgctgaa atcagtgggc tatcaaaaga accatcaaaa gtttacggat
    10381 cgaacatcgc ttactatgtc tctcaaaaag gaacaggcga tgtttcagct acttttggat
    10441 tgttagattt accaacagaa gtaaatgaca aaattcttgg ttacaaaacc gatacaaaca
    10501 aaatcagctt cttgggagaa gatactgagc caccatattg tgcaattttg atggaatcgg
    10561 aggatttgaa cggcgataca gcaatgcttg cgatgttcaa aggaaaattc agtcgtgaat
    10621 ctattaatct taacactacg acaaatgaag ctttcgaacc agaagcagaa gaatatgtat
    10681 tttcagcgat tgccaatgat gttgaaggtg atgctaaagg ccaaacagtc gcaaaatatg
    10741 ttggagacga agaagcttct attactggtt tgaaagagat ggtatttcct gcagcggggg
    10801 agtaactagc cccgttgttg gagcaatcac tcccactatt aatggggcaa cagttgaatt
    10861 aagttaggag gataactatg ccagatacat ttaaaatcta taaaaaaaat ggtactgaat
    10921 ttacaaaagt cgcagagggt gaaagtccac tttctattac tggaattgca gcaaatactc
    10981 aagttgctaa aggagattat caagctacgc gtgtggtcgg aggtcaagaa tcagtaaaag
    11041 tagatattcc tgcattcacc actcttccaa tttcagttac tggtgtgacg ttggataaaa
    11101 cgacagctga agttgaacaa ggcggaacct taaaattaac tcctactgta acaccagcaa
    11161 atgcaacaga caaaacagga agctggagca gttcgaatac agctgtagct acggttactg
    11221 gaggcaacgt tacagtaaaa acagacgctg aagttggcgg aactacagaa gtttcattta
    11281 ctactacgga tggcggtaaa gtagcaaaat gtactattac agttattgaa aaagcagcgg
    11341 gctagtcttc ggactagctt ttttatttgg aaaataatgg aggaatctaa atggcaaaag
    11401 taagaattga attgaaagaa gaatcaggaa agaaagtcta tgaaaacata gacacaacag
    11461 ggaaagatta tcgaaaagct ttagaaacaa tcaaaaagct aaatgaagac ggagtaatga
    11521 tttggaatcg tttagacatt tatttggatt ttgctgtagg tatcttcagg gatagcaaat
    11581 tgacagctga acaaattctt gatggcttac catctgagaa ggtaatgagc acattggacg
    11641 acatcctagg agaagtaatg gggattgaaa gtaatccaga cccagacgca aaaaagtaac
    11701 acccgaagaa gcagaagaaa tgtacttaaa cctatgcaga gagttagtga aagcgggatg
    11761 gtcactatct gacatagaag ataattcctt tgaaatgatg atgaaaattg cgtgcacttc
    11821 gcctaagaaa gagaaacaaa aagaagttga tcttaaagac ttcttgaaat ccatgtagag
    11881 aggaggtaaa catatggcaa atggaaaacc tttaggcaat atgattatca aactagactt
    11941 agacagttca gctttttcaa aaggtctagc cggagctaaa aacgctgtta accatcaaat
    12001 gaaagcaatg aaatcacaga tgcaagtaat gaactcttca ggaaattctc tcggtgcttt
    12061 acaagcaaaa tacaatggtt taggcggagt tctacaagca aatgaaaagc aagtagaatt
    12121 gctcactaag gcttataagg atagttttga ctctaacgga aacgctactg cttcgactgc
    12181 taaatacgct aatcaattga atcaagcaac agctagatca gcaagttacg aatctcagat
    12241 gaaaacaact gttggacaga ttgctcggat gaaagtggaa acggaagggt ttacggggaa
    12301 actaaaggct aattctgatg tattgattaa ttctgggaaa aaaattgaag cctttggtaa
    12361 aggagtttct gggataggat ctgccctaac tgttggagta acagctccaa tagtggcagg
    12421 cgcaactgct gttactagtg cagctatttc atgggaaagt gcttttgcag gtgtaaaaaa
    12481 gacaaatgat gaagttgtag actcgaatgg aaacgtagtt tattcctata gcgacttaga
    12541 gaaaggtctg cgtaatcttg ccacccaatt acctggtagc catcaagaaa tagcaaatgt
    12601 agccgaagca gccgggcaat taggtataaa gactgaaaac gtagttagct tcactaaaac
    12661 gatgatagac atgggtgaat cgactaatat gtcggctgaa actgcggcaa cttctttagc
    12721 acgatttgct aatattaccc aaatgtctca aaaagacttt gataaattag gatcagtcat
    12781 tgttgatcta ggtaacaatt ttgctacaac cgaatcagaa atcactgaaa tgggattgcg
    12841 gttagcggga gctggtaaac agattggaat gagccaaggc gaaattctag gatttgctac
    12901 tgctctatct tctgttggtg tcgaagctga agcgggtggg tctgcctttt cgaaggtcat
    12961 gatccagatg caactagctg tagaaaaagg atttggcgct tttgatcagc tgaaacagat
    13021 ggcagaagaa caaggtgttc cgtgggttaa tttagtaaat gctgtgagag acggtggaaa
    13081 atcgttaaaa gcagtatcag agcaaatggg attcacttca tctgatttaa agaaaatgta
    13141 taaagaagcg gacaattcga aaagttcgct tgaaaatttt gcgaatgtgg caggtgttac
    13201 aagtgaacaa ttccagaagt tgttcaagag tgatccttca gaagcaatca tcaagttcat
    13261 tcaagggctg aaagattcag aaaaacatgg tacttcagca atcaaagttc tggatgacat
    13321 ggatataaaa gaagttcgac tacgcgacag tttattgaga gctgcaaatg ctagtgacat
    13381 attcaaagga gcaattgaaa aaggaaacgg cgcgtggaaa caaaatacag ctttaacaaa
    13441 tgaagctaat aaaagatacg aaacaactga atcaaagcta aagatattga aaaacgaagt
    13501 gattgataca gcaatcgatc ttggaggtcc atttgtagac gcgttgcgtg atggtctaaa
    13561 tgtatcgaaa ccattgataa aaaacttagg gaatatggcg aaagctttct cagctatgag
    13621 tgatgatcaa caaagaaaca ttatcaaatg ggttggatta gcagcagcag ctggtccagc
    13681 gctgaagctt ttaggaactg gtacttcagt aataggtaaa gttacttcaa ctactggaag
    13741 attgacgaaa agccttgttg atttagcagc gaaagcagca gaaaagaagg ctatggatgc
    13801 ctttgcatcg tcggtaacaa cagctggtgc tgcagctgca aagacagcag gagctggagg
    13861 tattggcggg ttagtcccta tgatgggaaa aacagctagt gctgcaagta ctgctgcggg
    13921 cgccagcgga ataggcgcaa tgacaacgtc actaggtttg ttaggtcctg ctctacttgg
    13981 tattgttggc gttggtggcg cactggcagt tggctatgga gcatggaaat tatttggtga
    14041 agaagcttgg aattcctcgc aaagagtaaa gcaatgggga acagatgtcg gtgaggagat
    14101 agatagtact cttgacggag tacagaagaa tattgaaggt gcaaatggtc aattcggtct
    14161 tctcaaagag ggttttactg ttgatgatgc aaataatatg gcggctaatt tcgcaaccgc
    14221 tggaacatct ttggaaacat ctttaacggg tagaataaag gcattagacg atgcactaaa
    14281 agggctacct caatcagttc aggatgccat gaaagaagta gctgaatcag aagaagaaac
    14341 cttagggcaa tctctttcta aaataaaaga caacaatgaa gaaataaaaa aaatcagaga
    14401 aagagccgca aatcagaaca gagacttgac tgtttcagaa ctacaacaga tacaggatct
    14461 aatgaaagga acttctcaag cgtatgtgga tacgctcgat gtttctgcta aagacagaaa
    14521 aaagatacta tcagccatga atggtgatgt tgcacaagct acagaagaag aagcgaagac
    14581 gtggttacaa tctttagcga aacaaaaagc tgcaactacc gaaaattata gacaaatgct
    14641 tgaagaaaaa aaggcggctt taaaggaaaa gggagtaagc aaagaattca ttgatgcatt
    14701 agaagatgaa atgaatgatt acatcaaaac tacaaatcaa ggattcgata gccaaatagc
    14761 tgctataaca aataaatatc cacaattgat taacgaagtg agtttatcaa atggtcaatt
    14821 atttagcaaa acggcagctg ctatggacaa tactggtcag tatacaaaag tttttctcga
    14881 aagtaatgaa aagattttag cccaagcgaa gaaaacatcg gatgaaatag cagataatgc
    14941 cgaaaaaaat gcagaacaac ttggtatggt agctgatgag gctacgcatg ctggatcaac
    15001 ttggaatggc ttagttttag acgaaaaaac aggaaaagta aaaactaatg tacaagaagt
    15061 agtgaatgaa gcaacgaagg atttcactaa atggaatgaa ttgaaacctg ttgttcatga
    15121 tgcgaaacta acaacgaatg caaaagatgt catttccata gcggcagtac aaaatggcta
    15181 ttgggatgat atggaatgga aagaaaaaga agcattatta gaagatgagt gttctaaaaa
    15241 tgtatacaag gctttagaaa atgctggtaa atggaatgag ttagatattc cttctaaaga
    15301 agcaatccta acttcaaata ctcctgaagt tatgggtgaa actctcatga atttgggatt
    15361 atgggatact tacaaacttc aagtgaaaga tttagatttg aatgcgtata aagtatacga
    15421 tgctatcgat ggatcagaag aaaaattaaa tagttgggct caattagata accccacaaa
    15481 agaattgtta ttagataact cagatttttc tacaaaactt tttaattccg aagaaatgtt
    15541 taaacgttgg aatcagttat catctaatgc aaagtttctt tcggctaata atactaaatt
    15601 tacagaggtt cttttatcat ctgacagaat gtggaatcaa tgggtaaacc ttcctgataa
    15661 tgagaaaaga ttacttgcag ataacgaaga tttagctacc actgttttat cttcagaaga
    15721 aatatacgga agatggactc aattaccaga taatcaaaaa tatatgttag ctgacaatac
    15781 tgacttagca aacaaaatat tcgcttcaga agaatcatgg aatgcatgga aagcaattcc
    15841 agacacagtt aaaaacttgc ttggtaataa tattgattta ctggctaaag tgaaagacgg
    15901 gactgtaagt gtagaagaat ataacaaagt tatgccactt cttaaaactc tatatggaga
    15961 taacgttaat ttaaaagagg caatcagtgg ggctaaaagc tctatagaga attacaacaa
    16021 aaatattttt cctagtgata aaactgcagt aggacacgat gaggcttcac aagctgctaa
    16081 agatgcattt gaagcgttca acatcttcca aacaaaaata ccagataaaa ttactaaaac
    16141 tgtatcagct gactttatag gtcctatgcc gaaaaatgcg aaaggaacaa acttccatcc
    16201 tggcggagca gctatggtaa acgatcaaaa agggcctact tatgaagagc ttataacctt
    16261 gccaagcgga gaaactttta tacctaaagg acgtaatgtc gttttagact taccaagagg
    16321 ttcgaaagta ctgaacgcca ctaaaactaa acgtctagtg cctaaatatg ctgatggtat
    16381 aggaaacata acgacggttt catctacgct aaacttagat gctttaattt tagctattaa
    16441 cgagctaacg acggtattga gaactcaact gatggcagga acgactgatg ctaaagatac
    16501 taaactatca gatgaaacaa aagttgccga tccgattatt ccggacagtc tttctgaaaa
    16561 atcagatcag tatctaggaa ttggagctca atggttaact aatctaatga atggttggaa
    16621 ctcagcggtt cctcaatata tgaatagtga aatggttttt attacaaact atctcaatgc
    16681 gctaaaactt caaaacaatc ctaattatac gcaaggagca acatggaata agaacttact
    16741 gaatggatgg aatagtttaa ctgggacctt tattgctaca atcaattcat tttgtaatca
    16801 agcaatggtc acgcttagaa actacaacac tcctatgtac aacaatggcc gaacgtggca
    16861 gcaaaataat cttaatggtt ggaattcatt gtatggatca ttcatagccc gtgtaaatca
    16921 gcttggtaac gattcgatta acaaccttcg ttctaaaagt ggtggttttt acaatgcagg
    16981 gacattcttg ttgcaatctt taataaacgg aatgaattcg ttaggtaatt ctctttctac
    17041 tacaatgaac aatgtcgcta atacaatggt tggtggtata ggcaaaggag ttaatggcgt
    17101 catatcggga gttaactatg tgcttaagga agtcgaatca agtaagaata tcggaaactg
    17161 gccaattcca caatacgcta agggaactga tggacatcct ggtggacttg ctatgattaa
    17221 tgatcaaaaa ggcctagttc acgaagaata tgttcagatg ccagatggac gtagttttat
    17281 tgccaaaggt agagatcttc ttgttaattt gcctaaaggt gctcaggtct taaatgcttc
    17341 tttaacaaag aaattaaagg aacgtttaaa tattccacac tacgaaaaag gtattggcaa
    17401 tttagatatt gttgatttgt tagatgatga aaaaagaatg ttagaattct taactagcaa
    17461 agttgatttt tcaggcatta atgaaccatg gcttgatatg actaaatcag gaacgtcatt
    17521 aatgtccaaa gctgcaaata caatgcttca atcaaaatta agtgaattct tcactcatgg
    17581 caattttgat ggtgcagtaa atgccaatgg tgtttatcaa tatttagttg atgtggcaca
    17641 gaaagtaatg ggtaagttcc ccggacttac agtaacttct ggatatcgag caggagatgc
    17701 ctactatcac ggaaaacggc aagctatcga cttggcttat ccaggtattt ctggagatcc
    17761 gagatataca gcagcagcaa attacgcttt tgaaaaattc ccttcaaaaa ttgcgtatgt
    17821 cattacgaat ggacgtgtac gtgaccgcat gggattatct ggaacaaggt caagcggaca
    17881 atggacgaat tggccagatg gcgatcactt cgatcatatt catttaaacg gttcgatggg
    17941 gtctggcgat atattcaatg gaggagttgg aggaagtggt gttgcacgct ggcgttctta
    18001 tgtgtctaaa gcattgaaaa tgaatggatt accagctaca gcagcgtata taaatgcctg
    18061 gatgtcccaa atccaaacgg aatctggtgg taatgagaaa gcaatcggcg gcaatgatgg
    18121 gctcgccgaa ggcaatgcaa caggattact tcaaacgaaa cccggaactt ttgcagctaa
    18181 cgcttttcct ggtcacggaa atattatgaa cggatttgat aacatgttag cagcaatcaa
    18241 ttatgctaag aaacgttacg gagttgcagg tatgttgcaa gttatcggga aaggacatgg
    18301 atatgccaat ggcggcttga ttaccaaaga cggactatat cgagcaggag aaggaaataa
    18361 accagaaatg gttattccat tgacaagaaa aacaagagct atcgaactta tgggtcaagc
    18421 attagccttc ctttctggaa gcaataaaaa agaaacgaaa agtgagatta attctgtaga
    18481 taatagacat atcctacaag cgatagaaaa acagtctcgt atgacaaatg atctgctatc
    18541 attattactc tcgtttttta aaagtaacaa tcgatcagat aaagaactag ctttagatat
    18601 tcagaaaatt ttaatcagga ggacatagta tttgaggaca gtaatattaa gaaataaatt
    18661 gaatgaagaa attgatttgt ccacagaaga ttattttgct actgaaatgt cgaatctagg
    18721 ttttgaagtt acaaaagagc atattggaca atgggggagt ttcagagaaa taggagagag
    18781 tattgaattg tctgaatttc aatcatcagt tatgatttct gttcacggat ttagagaaca
    18841 acaattatat ggttcattag ttgaatttct ggctaatgga ccttttgaac tagaattttc
    18901 ttttgattca gaaacaatga tcaggaaatg tagtttgaaa tcattatcta aaacagaaat
    18961 tgattcccaa agctcattgc taacagatac tttgaatcta tattttactt caagctggta
    19021 ttctattaag cgtgaaaaat taatacagaa ttcaacttct ctcaatacac gtggaaaagt
    19081 ttactcattt gttcgaccat atgtctacac caaaaatgta tgggaaaaga aaggtgtatt
    19141 taagttcagt aataattctg tatatttgac aaatagtaca gagcgaatgt ctccgttaaa
    19201 aataaggatt attggagagt gctcaaatcc atattgggaa gttatccaaa actctaaagt
    19261 agtagcttca gatggctatt ttattaatat gaaagacaca caaatattag aagtgtctag
    19321 tttgctagaa gaccaaacgg caattttaaa agatgtaggt ggagttgaat cttctgttta
    19381 tcaacaacaa gattttacta aatcaaactt cgtccaagcg cctctaggag aattcagcgt
    19441 tgtttttcat gttggacatg caaatgtaga gattgaactt tatgaggagc gtgatctatt
    19501 ttgattttag ccattaccct ctttcaccga gatatgaatt tatataacga acgtttaatt
    19561 tcgactgagt tcgaatttgg aacggatgaa attggagaag aagtaagtaa ttttattaca
    19621 gataaatatg tccatgtaaa atcaggagat tttctactag caaaatttat tcctagcggg
    19681 aagattgctt attttggagt gattagctcg caagaagaca aaaaaataaa ttgcaaagga
    19741 ttactcagtt tggccgatag cgagataccg actgtaaaaa tatcaggtgc taattatgaa
    19801 gagcatattc gacgactgat tgaatactac ttattgaacg atccaacaaa acaactaaaa
    19861 aatattttgg aggtaaaaac agaaagcgtt acttctcatt cttatcaagc tgttgacaca
    19921 aataagagac gattgaatgc atatataata aatgggttca aaaaatataa tgttaaatgg
    19981 tatgtcaaag agattaaagg taaaaaaata tatactggta ttcgagcagt tgatgattca
    20041 atttatatta aagataattc atctgagttt aatgactggg atgtttttgt gcaatctcct
    20101 ggaccaggga acgagaacaa attattgatc gtagataaag caatgaaaga tatagaaaat
    20161 cccttggtat tgtctacgtg gtatttagat gacgaaaata atcttacgca agattcaacg
    20221 aataagaaca ttgcattgcc taccgttaat ttagtaaata tatatgatca aactcaagaa
    20281 gatagacctt cttatgaaga ggtagctaaa tcggagctta aaggcaacgc ctattcacat
    20341 gaaattaaag ttaatgttgt gctaaatgct agaaatatca atgtagaaaa tatcgaaaca
    20401 ggaatgttag cgactattgt ttacaaagag cagacttaca aatctgttct aactgcctgg
    20461 cgaatatcca gcagtaaaaa gaatatagaa ttgacatttg gtaatgtccg aagtcgtttt
    20521 atggattatt ttgaagataa tgggggataa aaaatggtta gcaatgtgga tggatatcaa
    20581 tttgaaaacg tgaaagtaag cgcagaaaat gatgctagac tttatcacgt tttatataat
    20641 cggaaaaatc aggttattga tggttacgat cagtctatga atttatcttc aagcggatta
    20701 acagtaaaag ttgctgcagg agcagcgatt attcaaggtc gtatggtcgt tgttcgacaa
    20761 gaagaaagta taacagttcc agcaaactca agtggttata tagcattaac agtggatttg
    20821 acacaagaag ttatacctgg atctattctt ccagaatcgg aagaatatga atggactaat
    20881 aatcaagtca agctagaatt tataacaaaa gttataaaag gtaatttaaa caatggtgat
    20941 aaggtctata atttaccact atgctcagtt aattctactg gatcaactgt ttcaatctca
    21001 aagatatcgg atagttacga gctgactctc tctaaaggag aaattttgtg gagggggact
    21061 gcgttaatgc atgatactca aactgtccaa ccttcaaaaa aaatttggca gacagtttca
    21121 ggttttttgt tagtatggct cccatacgaa aacgggcaag caattgagga tagatatgta
    21181 actacgcctt tttataagga gcgtataact tttactaatg ttttaggaga aattgtttca
    21241 ggatttgatg actatcacaa aaagtggttt agtaaacgaa taaactataa ttcgaatact
    21301 aatatattta caggtgcagc aagcaatgca agtggagata acgcaaatat ggtgcttagg
    21361 tatatagttt ctttttagtt aggaggtatg gaagagtggc aaatttagaa attaaattat
    21421 ctgcaaataa gagacagcct tatctacgtc accgtgttgt tggtagagtt ggtgatgggg
    21481 ggctcacaac aatcaatgta caacttcttg aggaagatga aattacacct tttgtaatta
    21541 atctaaacgg tactttgaaa tttgtgggcg aagtttcaaa cggtaactat accgagggag
    21601 aaccagaaat aatcgattcg actaatgggt taattagtta cacgttcact aagtcaaatt
    21661 tcagcacgag tcatcaattc aaacaagcat attttgaata tgtagatcct aacggcaaaa
    21721 aagtaacttt tcagaacttc atcatagacg tgttagaacg agtagatatt aattcggagc
    21781 aagcgaaata ctatatttct tcattggaaa aattacagag tgaaatgcaa accactttca
    21841 atcagttcat tagtgataaa caggtccaat acgatcaaat ctactcgaaa tataacgaat
    21901 tagtaagatt gataaatgaa tcagataagc aagtaaatga tcgtattgat aaggcaaata
    21961 atcgtattga tcaaaccaat cagcaaatcg gcgatctcgg caagctgaaa aagatgtaca
    22021 gtaacagcat tgatttcggg ggatatgatt atagcggtaa gccgaattta atgtccaaac
    22081 taaaatcgag cgattttaac gttggttacc acgggtcact aacttcggat aacgaaaagc
    22141 tacattttac ttctgatggt acaggaagca ttattatgtt tacgcgtatt aatacacctc
    22201 agcttgctag tgggaaaacc tatactctga gtgcgaaagt tcgatttgat gaaggaacta
    22261 caggagctat tgataaatta cgtttggtgt atcgtacatc accaggagaa aagatattat
    22321 tggaagcaaa tagtacaaat attacaacag atgatgtagg gaaagaaata acaatcaaag
    22381 gtacagctaa cgttaattat caaatcacaa atttagatcg attttatatg agtattagct
    22441 ttgttgacag ggataaaata aatggcggat ttaagttgta cgacatcaaa atcgaagaag
    22501 gctcaacagc cacaccgtac cagcctaact tactagatga cccttactgg gtaggtaaaa
    22561 catcgttggg tgagaatatt gcaaataaag gtacgacgtt cccaattaaa tctagcgcgt
    22621 acgaaatata taaaggtaac acggaagaag agcttataat aggtcaaacg tatactatca
    22681 cgcttaaagg aacaaaaccc gcaagtcaaa cctttgtagc atataattat tggaacgtta
    22741 attttggaga actaaaacca gttgagggat tgacagacgt atggtctcta acattcacac
    22801 caacgaaact tgaaccgggt ttgcctaaag accttcgcat ttttcagttg cctaaagaaa
    22861 cggccggcgc atgccaaatt gactggctca aaattgaaaa aggcgacacc cgaaccccga
    22921 atattagtca gtttaaatac tttggtgaag gattgaaaga tagcaacaat ccgaacgact
    22981 acagctggga catcacgcct gaatatactg aaaaaggctt gaatgatacg gttagtttga
    23041 ctgaaccgca atccatcgaa ggattgaaaa atttcgaaga tggtattcaa tcaaaaggaa
    23101 aatctgtatt gacatcagac gacaacaaat atgaagtcgt aaccttaaca gttacaaacg
    23161 ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac cgtcactatt tatttttttg
    23221 cgttgaatgg gaaaagcagt ggtggaaatg attcaacgat actaacaatt ccagaaggct
    23281 atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct actttgaaca
    23341 gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac tcaagttatg
    23401 gatcagctta ttcatttgtt atcacttatt caatttaagg gaggaaatct aatgaaagta
    23461 gtttacaaat caatcaagcc ttacggattc gagcaaatca ttgtgaatga cgaagaacat
    23521 ttaccggaag aatgcacaga agtcgaacca ccgattccaa attggaaacc gaaattcaat
    23581 tactgggagg gaaataaatg aaaaacattt ggaaatatgg ccgtactggc ggagagtatg
    23641 caggaaaagt attggacgac atgcttgtat ccgttcctta cacagatcag ccaccgctcg
    23701 aaggggttcg ttcagatggc gaaccgctaa cgattgctga tcagatgttt gatcctaaat
    23761 tgaaccaatg gattatttta gcgaacgcgt tagatcacaa cgatttaaac aatctcaaag
    23821 cgatgtacga ggctcttgaa catgaaaacg gcaacctaaa acagctaaat gccaaactca
    23881 tgctaagcga tgtagcgatt aaacaggaaa atactgcatt gaaagaaaaa gctgacagtt
    23941 tagcacaaat caattcaaaa atgatgcttg cttcgttaca aaatagcaaa gacatttcag
    24001 aaattaaaga gcaactaaat ccagcttcaa aaggaggtga gtagtatgtt tagttttagc
    24061 gatgtgaaaa tgatgtatga ttggggctgt tttactgacg atcaagttcg actattcgtt
    24121 ccactatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
    24181 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
    24241 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
    24301 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
    24361 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
    24421 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
    24481 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
    24541 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
    24601 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
    24661 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
    24721 gaggaaacaa atgaaaaaga aaattactat tactgcgatg agcctgttaa cggctctttt
    24781 tttattgcca attaacggat ttgcctatac tatcaacaat gaatttaatt tgggcgcaaa
    24841 tgaaggtagc tcacaagtag caaataatca gtatatttta ctgcatgaaa cggctaatga
    24901 aacagcaaca ggacgcaatg aagcgcagta tatgcaacgt tcatggacta gcgcttatac
    24961 tgcttatatt gtgggagacg gcggaattgt ttaccaagtc ggacaacctg gttatgtaca
    25021 gtacggtgct ggttcatatg ctaatgccaa tagtcctgtg cagattgagt tacaacacac
    25081 acatgataaa gcaacttttg aaaaaaacta taaagcatac gttgaattgg ctagagattc
    25141 agcaatgaaa tatggtattc cattaacatt agacacgcct tataaccaac caggaatcaa
    25201 atcacattta tgggtaactc aaaacatttg gggcgatcac actgatccgt atggatactt
    25261 atcagaaatg ggtgtaagta aggaaaaact ggcttacgat ttagctcatg gatttacaga
    25321 cgaaaaccca acgacttctg agaacaagcc tgtcattgat ccaacccgag caggtgcagc
    25381 aaatccaact ttatcagatg gaacgaacca ttctcacatt gatcagtttg gagaaatcga
    25441 aaatgcgaac ttacacgtcg ctggatggca catcgctaac tataagtacg agtatatttt
    25501 cattatggac tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag
    25561 accggatgta aatcaagctt ataatacttc tggaaatgtt ggctatcatg tatcttttaa
    25621 catgcgtaac ttccctaata agaaagtcta tgtcatgatg cgggcaacga atgatccaga
    25681 gggaaacact aaaggcggtg cgcaagattt ccatgacaaa cgttggtatt taaatattcc
    25741 taaacgataa aaatagctcc tcgttgagga gcattacata actatattga caactataaa
    25801 aatcattcga taaaatagtg atgttatcgc atatcttcac tatcacccat aatagtcaca
    25861 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
    25921 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
    25981 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
    26041 ttagaatttt ttgaagagta ttatacaagc taaagctttt cttcattgcc actcaaatga
    26101 gtggcttttt tatgtatcct tttatggatt aatgaaagga tgtttcacat agttatattt
    26161 ctgtatattt gaaaagtttt actttgattt ttaaatagaa agacatttgg gttatattgt
    26221 gagataataa taaagaagag tttaaagcgt tccccaaaaa ccactgcccc ataagtgtgt
    26281 tacgctttaa actcttttat atttgaagcc attaaaaagc ataccatatt tttgaaaaaa
    26341 agtgagaaaa aaggcttata attggagtgg tag
//
LOCUS       DARBER010000023        25795 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARBER010000023 DARBER010000000
VERSION     DARBER010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.023"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            221..2281
                     /locus_tag="SCK06_001008"
     CDS             221..2281
                     /locus_tag="SCK06_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG1151028.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2364..2933
                     /locus_tag="SCK06_001009"
     CDS             2364..2933
                     /locus_tag="SCK06_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151029.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3108..3914)
                     /locus_tag="SCK06_001010"
     CDS             complement(3108..3914)
                     /locus_tag="SCK06_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151030.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4052..5566
                     /locus_tag="SCK06_001011"
     CDS             4052..5566
                     /locus_tag="SCK06_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG1151031.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5969..6952)
                     /locus_tag="SCK06_001012"
     CDS             complement(5969..6952)
                     /locus_tag="SCK06_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG1151032.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6957..7604)
                     /locus_tag="SCK06_001013"
     CDS             complement(6957..7604)
                     /locus_tag="SCK06_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1151033.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7689..8321)
                     /locus_tag="SCK06_001014"
     CDS             complement(7689..8321)
                     /locus_tag="SCK06_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1151034.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8337..8888)
                     /gene="coaC"
                     /locus_tag="SCK06_001015"
     CDS             complement(8337..8888)
                     /gene="coaC"
                     /locus_tag="SCK06_001015"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG1151035.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8885..9622)
                     /locus_tag="SCK06_001016"
     CDS             complement(8885..9622)
                     /locus_tag="SCK06_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG1151036.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9619..10608)
                     /locus_tag="SCK06_001017"
     CDS             complement(9619..10608)
                     /locus_tag="SCK06_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1151037.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10866..11408
                     /locus_tag="SCK06_001018"
     CDS             10866..11408
                     /locus_tag="SCK06_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1151038.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11425..12510
                     /locus_tag="SCK06_001019"
     CDS             11425..12510
                     /locus_tag="SCK06_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151039.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12522..13328
                     /locus_tag="SCK06_001020"
     CDS             12522..13328
                     /locus_tag="SCK06_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151040.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13329..14156
                     /locus_tag="SCK06_001021"
     CDS             13329..14156
                     /locus_tag="SCK06_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151041.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14153..15226
                     /locus_tag="SCK06_001022"
     CDS             14153..15226
                     /locus_tag="SCK06_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1151042.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15654..18350
                     /locus_tag="SCK06_001023"
     CDS             15654..18350
                     /locus_tag="SCK06_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1151043.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18427..18915
                     /locus_tag="SCK06_001024"
     CDS             18427..18915
                     /locus_tag="SCK06_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1151044.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18927..19421
                     /locus_tag="SCK06_001025"
     CDS             18927..19421
                     /locus_tag="SCK06_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1151045.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19442..20251
                     /locus_tag="SCK06_001026"
     CDS             19442..20251
                     /locus_tag="SCK06_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1151046.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20248..21069
                     /locus_tag="SCK06_001027"
     CDS             20248..21069
                     /locus_tag="SCK06_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1151047.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21120..23219
                     /locus_tag="SCK06_001028"
     CDS             21120..23219
                     /locus_tag="SCK06_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG1151048.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23216..24334
                     /locus_tag="SCK06_001029"
     CDS             23216..24334
                     /locus_tag="SCK06_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1151049.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24373..25251
                     /locus_tag="SCK06_001030"
     CDS             24373..25251
                     /locus_tag="SCK06_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1151050.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25442..25594
                     /locus_tag="SCK06_001031"
     CDS             25442..25594
                     /locus_tag="SCK06_001031"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1151051.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8175 a         4477 c         5437 g         7706 t
ORIGIN      
        1 aaagaaaagg taactagata taactagata taactagata tattacttaa caaaattaaa
       61 aagtacataa gttattattc cgattttctt gaaacaataa aaaatcaaaa aaaatactct
      121 cctaatagtc catcttcttt tgattttatt tatataatga aatcatctta tgaaaattac
      181 tacttcatgt ttagacaaga gtagggagga ctaagaataa atgtgttcaa aaagacgttc
      241 aaaacaacta aataaacacg cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca
      301 ctacgttaag tttatgatgc gcacgatgct gatcagttta tctgtaggtg tctatatgca
      361 tgtacccgtt caggcaacga cacaaaccat caaggtcgta cgtacctctg tttctgagac
      421 cactgattcc cctatcacta ttactcatac tttcactgat gcaaatggaa aacagctact
      481 tcctgcattg acttcctata ataaagtcag ttatcctcca acgataaacc actactggtt
      541 aattcctaac aaaacattat ggacatcgga aaccggaagc agtgtgccgc tttctatctt
      601 ttccgatatg actgatccta caaaaggtga tcagttaaaa cagatcactg atattttgaa
      661 tcatgctttg acagatataa aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa
      721 agattattca cagtttgaaa gaaagaaaat cgatgttctg gcagggacaa aagtttcgat
      781 atctgatctg gtagaaaggc tgaagaacgc agatggaaca caaggagata tgtccaaagt
      841 aatttcaaac aatgggaata tcgtggatac gactaataca ggaacatcta ctatttcctt
      901 gtcctatttt gatccaattg ctttaaaaac aatgactgca tctgctgtga tccatgttcg
      961 tgctgatcaa acagctatca aaggaaaaga tcttcactta agaacaggag agagttacgc
     1021 ccctaaagat cttgttgaat atgtaacaaa aagcgatgga acttcaggaa ataaagaaga
     1081 aatcaaaatg aacgggacag tagatactac aactccggga gaatatcccg ttgtattaac
     1141 ttatatagac tcgaaaacat caaatgtggc agaaacaaca gctgtagttt atgtagaagc
     1201 atctgctgct tcatcgactg tcatcactca ttttatagat agtcaaacaa ataaagaact
     1261 tcaagcagaa acacatatga gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga
     1321 attttctgat cgagtgccaa atatagattt atcgacttat gaacaaaatg ggaaagtcac
     1381 ttctatttct gattggatgc aagcaaacgg attagacaga aaaagtcact ggtcggagat
     1441 ccttgaagta cttcactcag aactgatgaa taataggaag caatcaggag gggaaattac
     1501 ctttacttat gtttatatac cagacaatcg ttcattagaa ggtcatgatc ttattgttca
     1561 tccaaacgaa aaaataaaca aagaagagtt gatccaatca gcaacaaatt ccttaggtaa
     1621 acccgtaccg ttaaaggatg tatcggttac aatcaatgaa gtaccattgg aggatggtta
     1681 tttgaccaaa caaacaggtg attttacgat tatgtatagt tatcatgacc cgtattcact
     1741 ccaagaactc catgctcagt cactattaca tgttttacca ttaaacaaac agatagatca
     1801 aaaagaacaa aaaagacgag aagaaagctt gtcagaaaaa aaacaacaaa tggctcatgc
     1861 agtttctcca acagggaaag taaaacaaac aagaagccta gagttagcaa gaatatctag
     1921 aaaaatctca gaatctaata aaacgataag gcaagaagct catttggccc cacaagtttc
     1981 tggattggaa aatgaaattt tagagcctaa aataaaacaa caatcagaaa aagaatttgt
     2041 tgctaaacag tcggaagatc agaaggaatc agatgaccag aaggaaacga agaaaaaacg
     2101 cttgtcaact attgattcca ccgatgagca tctgcagccg caagtaccac ttcctagtcc
     2161 gggaacaccc gcacctggtg gaggtattcc ttcttctgct ttaggagatt tcatgcgagg
     2221 tatggcagga acatgggtat acgctaatcg aaatgaattt gacgcagatg actggttctg
     2281 atttatatct ctatctgtta tagtcaaata agtagctgag tcatatgtta tttggcataa
     2341 tggagacgag gagggataga aaaatgttag ataaaacgtt accttacgca gaaatctgga
     2401 tgacaagacc acttcaacaa aatgttccaa tcacgccttt agcggaaggt tttgaatttc
     2461 gattttatca aaaagaagat gaatctgctt gggcggagat tgagacttcc gttggggaat
     2521 ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag
     2581 aacttgaaag aaggatgttt tttgtagaga caaaaaaagg agaaaaagta gcaacctgta
     2641 ccgcttggcg aaaaaaatca agagagcaag tgtatccggt ttttcattgg ttagcagtga
     2701 aaccagatta ccaaggaaaa gggctcgcaa aagcattgac tgccagagtc ttgcaagaat
     2761 ttcctaccct ccatacggac ggaccaatct atcttcacac acagacatgg agccatcagg
     2821 cgattgctct ttataaaaaa atgggtttta cttttattgc tgaaaatcta gacggcacac
     2881 ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa attagataaa taacgaaatg
     2941 gtgaaataag tcctcagtat tgggataaaa aacgaaatag tcattcctca tatcttactt
     3001 ttttgagtta atactatcgg ataactaatg gcatgccata cattcaaaaa agaaggggca
     3061 gtgacaaaag tattgtcact gccccttctc ttgtttttta gcatgtttta tcgtttgatt
     3121 tttttaggaa taaaattctt gatgaacgta aatttttggt aatcgtttcg actatagagg
     3181 taaccaaata cactgaaaac aactgctcca ataaaattaa caaataaatc cttcatagta
     3241 tccatgattc ccacgtctaa gtaaccattt tggatgattg ttttatctgt tttaccatct
     3301 gcattttgac tttcaatgat tgtccgagtg atatctggaa catgatagac agtgtttccg
     3361 tctttactta actccacact attgatttgc tggatgatcg tatctttttg catatcgaat
     3421 tgcagtaatt tatctgctga aaattccaca aactcccaca taacaccaac tgtcatcgaa
     3481 aaacagaagg aaacaactgc tagtaataaa ggagtcaagt tcatggtttt tgtattttta
     3541 ttgagcaaat aaacaaggga aaagccgaca cccccagcta aaaatccgct gaatgcatgt
     3601 agaaaagtat cccacattga aaaatagcca taaaaatcac ttaactctcc taaaactgtt
     3661 gaactaaaaa taaaagataa caaaatgatt tctagcatat ttggaatctg tatctttaat
     3721 atcttttcca gaaaataagg gatttcaaat aaaattaatg tcagtaataa aagcattgta
     3781 tttccccagt gttggttcca gacctgatcc actagcataa taacgacgat gatacgtaat
     3841 aaaatataga ttccgtttgt gattctacct gcagtttttg tactgttcgt aaaaaaggtt
     3901 ttcaaatgat tcataaaata actccttcat gtatatcctt aattatacta agaagcgtgt
     3961 gtttaccaaa gctatatgac ttaaagaaaa acagagttga tattgtgaaa aaaatcactt
     4021 aatatagtat aaggagaaaa gggggataca aatgaaaaaa ttagttgctg gtttattaac
     4081 agccgttgca cttcttgcta tgagcgcatg cggcgcagat cagaaaaata cagcgcagac
     4141 aagttctagt acgaaggaat ctgccgaagt atcatcaggc gcttctgaac aagtatatac
     4201 agatccaaaa gaaatgaaag aatcttatga tgtcgtgatt attggagctg gtggtgcagg
     4261 gatggcagca gctatcgaag caaaagatgc tgggttgaat ccagtaattt tggaaaagat
     4321 gcctgttgcc ggaggaaaca catctaaatc atcaagcggt atgaatgctt cagaaacaaa
     4381 atttcaaaaa gaagaaggaa tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa
     4441 aggtggtgga ggaacgaatg ataaagaaat gcttcgatac tttgtcgatc actctgctga
     4501 tgcggtcgat tggctagatc aaaacggtat caaactggac aacttaacga tcactggagg
     4561 gatgagtgaa aagaggacac atcgtccgtc tgatggctcg gctattggtg gatacttagt
     4621 ggaggggtta ttgaataacg tccatgagcg ggaaatccct atttttgtca acgcagatgt
     4681 gacagacatt aagaaaaatg gggaaacggt caatgaagtg actgtacatg ttcaaggaga
     4741 agaaccaaaa gaggtcaccg gaaaagcaat cgtagttacc acaggcggtt ttggtgccag
     4801 caaagaattt attgaaaaat atcgtcccga tttagctgat tatattacga caaatcaaga
     4861 aggatcaact ggagatggta tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat
     4921 ggataaaata caaattcatc caactgtgca gcaggatgag ggggtcttga ttggtgaggt
     4981 tgttcgcgga gaaggagcaa tcttggtcga tcaagagggg aaaagatttg tcaatgaaat
     5041 ggatacaagg gataaagtat ctgcagcaat tacagcattg cctgaaaaat cagcttattt
     5101 gatttttgac caaggcgtgc gtgatcgtgc cacagctatt gaattttatg accaaaaagg
     5161 atatgtcaca actggaaaga caatggaaga attagcagaa aaaatcgatt taccaaaaga
     5221 aacactagca aaaacagttg atggatggaa tacggatgtt gcggagaaaa aagatgctca
     5281 attcaaccgt gccacagcca tggaacatga cttatcgtct ccgaattatt atgcgatcaa
     5341 aatcgcacca ggaatccatt acacgatggg cggtgtgaaa atcaatacga acactgaagt
     5401 attagataaa gaaaaccagc caattagggg gttatatgct gctggtgaag tcacaggtgg
     5461 cttacatgga aacaaccgga tcggtggaaa ttcagttgcg gagatcatta tttttgggcg
     5521 acaagcgggc atacaagcag ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat
     5581 aaaaagaggt tgtgaaatca gcgttttgag ctaataccga gggtcagctg attgaacact
     5641 gtttagtgaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
     5701 aaacagcttc ttatgttttt aacttggttg gacttattac cgcagatgca agcttttgaa
     5761 caccgtttat tcggaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc
     5821 taaacacgta taaacggcgg aagcagaaga aattccttcg gaaataagct gaaattcaca
     5881 aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt
     5941 cccagcctct tttttgatca gcatgatttt attcagttat cttcaaaatg atttttccga
     6001 tgtttttgtt tgcttccata tattcatgcg cttgtttgac tgcgctgaat tggaatgtac
     6061 ggtcaatgat tggccgaatc ttgccatttt ctagaaaagg cccagctttt tgcataaaag
     6121 tttgtgcgag ctctgcttta tactgatcac ttctaggtgt caataatgta ccaagcagtt
     6181 ggacacgttt cccaatcaat acaccaaggt ctatctcagg cacgatggtc ccacctagca
     6241 taccaatcaa gacccaacgt ccatcaacgg ctatagaagc taaattcttt tcccagtaag
     6301 aagcgccgat gaaatccaaa ataacatcga ctccccgacc ttcagtgatt tttcgtacct
     6361 cttcttgaaa gtcttgtttt ttatagttga tcaattcatc tgcaccaagt tctttacaaa
     6421 aggcgagttt ttcttctgat cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag
     6481 ctaattggat cgctgctgtt cccacaccac ttgctcctgc atggatcaag acagtttcgc
     6541 cttcttgtaa ttttccaagc cagtaaagtg tttgataagc agtcaaaaaa acttctgaga
     6601 ttccagcagc agaaacatag tctagcgtat cagggagaag gattgcacga tcggcaggca
     6661 tcacagcata ttcggcataa ccacctagat tgactaatcc gtatactttt gtcccaggag
     6721 taaaagtgga gatatcactt ttattctcaa ctacctctcc agctacttcg actcccaaaa
     6781 tagggtaggg aggtttcaaa gagactgatt ctcttttcat aatatctgtt cgattgacag
     6841 ctgctgtatg gactttgatc agtaattcgc cttgttttgg tttaggaaca ggcacttcaa
     6901 tttgttctaa tacatctaca gaaccaggat gcttgattga aatggcacgc atggatctat
     6961 tcctcctttg cccattcttg gaatgctgta ttcaactctt tgctaccttc agaaagtact
     7021 agagtcgtac gagaaatatc cgtttcttcc actagagcgg caagtacagc agcaactgta
     7081 gagcgagtga tcgtttttga tgccttctca ttaggcttag ttaattgaat ggaagtaact
     7141 tcttcatcgt tagtcaatgt gactggttga agaatgacaa aatctaagtt agttcgtttc
     7201 agccattcat ctgcataatg tttagtaata taataatctg tcatggactc aggccatttt
     7261 gctggttcat ctgcataaac agcactgacc atcagatagc gagagacatg cgcattttca
     7321 gctgcgatca ctgttttgat tgcgccatcc aagtctactt gcagaagatt ctttccttga
     7381 gagccggcag caaaaatcaa tacatcgatg cctttgaatg cttcggtcat tttttccacg
     7441 gaccaagtta aatcaaaaga gacatacgaa acattctctt tttttactaa tgtttgggaa
     7501 gcaacatctc tcacccctgc aaaaatttca tgggaacttg gagctaaatc ttttatcagg
     7561 tgtcggccaa tttgtccgtt tgcacctaca acaaaaattt tcatttggat catcctttcg
     7621 acgtcaaatc tttccattca atagtaacac caaaagagaa aaaaatcaga gatcggctac
     7681 gatgactatc agttagtaag agaagggtgc ttgaagtatc ttaacaagac attgcatact
     7741 gctgcagtcg caagacctgc gacgattcct tcaggtatac cacttgtaaa aataaccgct
     7801 agaatagctg aataaacagc atttaaattt gttccgatga ctttggcata atcttgctgg
     7861 aataaaaagt agatcatatt catcactaaa atcgtattgg tcaatgaacc ggaaagtcca
     7921 gctaagaata aagaaacgga atctgctttt tgtttcatta gtttgcgcag acctttataa
     7981 acgaaataag gaaccacccc aatcaagatg cgaggaataa acgcgatcag caaggctttc
     8041 cagcttccat gttctgttcc gatgactggg ataaacggag aaaagacaaa agacaaaggt
     8101 gtttggataa ctgtgctacg gatcatgctg atcagtccaa atgtaccgcc aagaaaagca
     8161 cctagtcttg gtcctaaaac gatcgaagca atgattacag gaatatgcat ggttgttgca
     8221 ttgattggac cgatagggat aaaacctaaa ggcgtaacag ctaataaaat caaaatagct
     8281 aaaaacatcg cagttaatgt aaagtttttt gtatttttca taagtactcc ttttttctaa
     8341 gcatttcttt cattaagagt cagcatgacc tgttcgatga tcgtctgaat atcagctaag
     8401 gcaccttttc caaaatctcc acaagctaaa agagattctc ttggttcaat ttcctgatag
     8461 ccgacagatc tcaatgtttc cagattacgc tgagtgatcg ggttttgata catattcgtg
     8521 ttcatggcag gggcaatcaa ttttggtgtt cccactggaa gcgccattgc gactgttgaa
     8581 agaagatcgt ctgcaatgcc gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg
     8641 atgagaaaca agtcagcttg ttttgctagt tcgatatgat tgattacctg cggatttgat
     8701 tcttgcatga cgtctgtatg gactgcgcgc ttcgacagag attgcaaggt taatggcgtg
     8761 ataaaatgtg tgctgctttt agacataatc acatctacct gggcacctaa tttgacgaat
     8821 tgacttgtaa gatcagcgct tttgtaagca gaaatacttc cagtaacgcc tagtaagatc
     8881 tttttcattt tgattcctcc agtctaattt tctcagtaat caatgcagca atctcagatt
     8941 tactttgagc ttcttccact gttccatctt tagataagag atagccgtgg tgtgtctcgc
     9001 catggatttc cgtcaaatca tttgcgaaaa taaaatcagt tttgttattt atcagactat
     9061 tttttgcgac tgccagaaga gattcctttg gtacatctac taatagtttg aaaccaatga
     9121 tttttgcttt tggctgccat tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa
     9181 cgagcacaag atgctcggtg ttagaagaga tctttgaaac agtggtatct gtctgttcat
     9241 tttccgcaaa ccaattgtcc agttcttctt gcgttgaaga agtgggtagg ttttttgcca
     9301 gctgttcttc tgaaaaacta aaagcaggag taaaatcact gactgccata ctgtggataa
     9361 tcgcatcgta gtgttctttt ttcgttagtt cttctaattg taaaaataag tccagtgcag
     9421 attcgatcgt ataaatcgtt atattggagt gtcttttcgg tttcagtgct tgtttcgtag
     9481 tcacgtaatc gattgtggta tccggattgg cagctaaata atcagctaat gcttgcccta
     9541 atcgtcctgt cgaatgattt gtaatagcac gtacttgatc gattttttca gtcgttcctc
     9601 cagctgtgat tagaatcttc atttatggtc acctacattt actagtgttc ttccttggtg
     9661 tgttccagca agtagtttag atgctgtttc aggcaattga tccagagtaa tctcttggac
     9721 gaccagttca tctgcaatct gcaaatccgc catctgttgc cataaagaaa gacgttgatc
     9781 tattggtaca ttgacagaat caattccaat caactggatg tttcgcagaa taaaaggcaa
     9841 gacagtcgcg tttagtttga ttcccccagc attgccacat aaagctactg caccgttata
     9901 agagatcaat ggaagaagac tgcttagctg ctcgccacct actgtgtcga tgacatagtc
     9961 gatttgctgt tttcctaaag cttttgttgt ctctggcaga aattcatcag gtcgtacgac
    10021 ttgcaaagca cctagacttt ttagccatgt gacagactct tcttttctgg aaaaggcaat
    10081 gattgactgg aatcctagtt tatgtaacag ggctatcgct gtactgccta caccgccaga
    10141 agcaccagta acgacaactt ttttatcgga ggtcatacct tgacggatta aagcgttcac
    10201 acacaacata gcagtaaacc ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt
    10261 ttttggtaga ggaactaacc agtctcctgg tactttttga tattggctat aaccgcctgg
    10321 atgggtgacg ccaagcccaa aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg
    10381 attgttactt tggacgacag ttccggataa atcaatccct ggagtcatcg gatatgttcg
    10441 tatcacctga ccagactcgc tcattgccaa tgcatcttta taattgacat cagaataggc
    10501 aatttttacc aagacttcat tttcagccaa ctcagtaaat ggcagatcac tgattgctgt
    10561 atggaaaaca ggttccttta ttacctgaaa agatcgaaaa gcgttcattc gtgagacact
    10621 ccttttaaaa ttttctttta tcataccata aagcctactc ttttattctt gttcttccgt
    10681 aaatttagac aaaaattagg tgaaaaataa agaatcaaac aactagaatt tattccaaaa
    10741 gataaagaaa tattcagcaa aaaaaatttt acaaagggtt gcctttttca ataaatttcg
    10801 tatactaata cgagtggtag taaacaggaa gtttactatt agaaaacttc gattcggagg
    10861 aaattatgga aattggtgag aagttaagaa atttgcgcat ccaaaagaat ttgacacaag
    10921 aagaactagg cgaacgcaca gatttgtcaa aagggtatat ttcccagttg gaacgagatt
    10981 taagttcacc atcaatggaa acattctttt ctattcttga agtcttaggt gtgactccag
    11041 aagaattttt tcatcaagaa gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata
    11101 cggtttattt cgatgaagaa aacgggtatg aattggaatg gctcgtggct gattcgaatg
    11161 aaaaagaaat ggagcctgtc ttgctgactt ttgaaggtgc aggagaatat aagacgtttg
    11221 aaccttcatt atctgaaacg tttatttttg tattagaagg ggaattagag ttaagcttag
    11281 gagagacggt ctatgtggca aaagcaggac agtcgatcta ttatcaagcg acaaaacaac
    11341 atcaattaag gaatcatctc aaaaaacgaa caaaagtatt gattgttgca acgcaatcat
    11401 atttgtaata tagggggtaa aaacgtgggg aaaccaatca tttcatttga tcaagtagtg
    11461 aaacgctatg atgatgaaac agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa
    11521 ttttatacac tgcttggtcc ttccggctgt ggtaaaacaa cgatcctacg tattatagct
    11581 ggattcaccg aagctacaga aggggacatc tattttgaag ggaaacggtt gaatgatttg
    11641 ccagctaata agcgacaagt aaatacagtc ttccaagatt atgcactttt tcctcatatg
    11701 aatgtttttg ataacgttgc ttttggattg aagatcaaga aaatgtccaa atcagatatc
    11761 gaaaagaagg taaaagatgc tttgcgtatg gtccagcttc ctggatacga aaaacgggag
    11821 attagtgaaa tgtctggtgg tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat
    11881 gaaccgaaag tattgctatt agatgaaccg ttatcggcgc ttgatttgaa actacgtaca
    11941 gatatgcagt atgaactgcg ggatctgcaa caacgattag gaatcacgtt tatttttgtt
    12001 actcatgacc aagaagaagc gttggcaatg agtgatgaga tttttgtcat gaataaaggg
    12061 aaaatcgtcc aaagtggtac acccgtagat atttatgatg agccaatcaa tcatttcgtt
    12121 gctgattttg ttggggagag caatatcgtg gacggtgtaa tgatcgaaga taatctagtt
    12181 gagtttgttg gtaagcaatt tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt
    12241 gaagttgttt tacgcccaga ggatttgacg atcacgacac cagataaagg aaaactagtc
    12301 gtaacggtcg atactcagct gtttcgtggt gtccattatg aaatcatctg ttatgatgaa
    12361 cagcaaaatg aatggatggt ccattcaacg aaaaaagcta aagaaggaag caaagtagga
    12421 ttggcctttg agccagaaga tatccatgtc atgcgtttca acgagtctga agaagaattc
    12481 gatgcacgtc ttgacagcta cgaagaatag gggggacaag aatgaaaaca atgcgcagga
    12541 tctattccgt cccttatatt ttgtggctcg gtttattcgt gatcgcgcct gtattaatga
    12601 tcatttatca atcttttttt gatatgaatg ggcaattcac gctgaataat tatctatctt
    12661 attttacgtc ggggacatcg ctttctatga cgatcaattc tgtatggtat gcatttttga
    12721 tcacgttttt cacgctgatc atcagttacc cgactgctta ttttttaaca aaactgaagc
    12781 acaagcaatt atggctgatg ctgattattt tgcctacatg ggtgaatctg ctgcttaaag
    12841 cttacgcatt tattgggatt ttcagtgtgc acggcagcgt gaatcatttc tttgatttta
    12901 tcgggatcgg gccgaaacag atattattca cagacttcag ttttctgttc gtggcaacgt
    12961 atatcgagat tccatttatg attatgccaa ttttcaatgc attggaagag atgaatcctt
    13021 ctttgatcag tgctagtaga gatttaggcg cgaatagctt tgagaccttc cgacgagtga
    13081 tttttccgtt atcattaaat ggtgtgaaaa gtggtgtcca agcagtattt attccttctc
    13141 tttctttatt catgctgact cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg
    13201 ctatcgaaca gcatttcttg gttacacaaa actgggggat gggttctaca atcggtgtga
    13261 ttttaatcat tgctatgttc atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc
    13321 gctcataaat gaaaaaattt aaatggtcgt atctttattt agggatcgtc tttttattct
    13381 tatatgcacc gatcttctat ctgatttttt attccttcaa tgaaggagga acgatgaaca
    13441 gctttacagg atttacttgg gaaaattatg aagctgtttt tgaagataca cgtttgatca
    13501 ttatcgtatt gaatacgttc atgctggctt tcttatctgc attgatcgcc acgatcatcg
    13561 ggacatttgg ggctatggga atctattaca cgaaaaaacg acgtgcacgg acagcgttgt
    13621 tgagcatgaa taacatctta ttagtctctc ctgatgttat tatcggtgct agtttcttga
    13681 ttttctttac actactagga ttcggattag gttttcagag tgtattgctt tcccatattg
    13741 ctttcagcat tccaatcgtc gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt
    13801 caatggtcga tgcggctaga gaccttggtg ctaataattt ccaagtagtc aaaaatatca
    13861 ttttgcctta tctttctcca ggaatcatag ctggatattt tatggcattt acctattctc
    13921 ttgatgattt tgcggttaca tttttcgtca caggaaatgg ctttacgacg ctctcagtag
    13981 agatctattc tcgtgcaaga caaggaatca gtttagaaat caatgcgcta agtgcgttag
    14041 ttttcttgtt ctccatgata ttggtcattg gatactactt tattagtaaa gaaaaccgtc
    14101 caaaacggct gaagaaaaac agaagagtac gcgagggggt ggcacggctg cgatgaaaaa
    14161 attacaatct ctgtttttag gtattctcat cattattgcg gtgctgctgc ttggtgttcg
    14221 tcaacttgaa aaaacaagtg gcatgagtgg agcaaaggta cttaccatct ataactgggg
    14281 agattatatc gatcctagtt tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa
    14341 ttatgaaaca tttgattcta atgaagcgat gttcacaaaa atccagcaag gtgggacaaa
    14401 ctacgacatc gctataccaa gtgaatatat gatccaaaaa atgatcaaag aaaaactagt
    14461 acttccatta gatcattcga agatcaaagg attaaataat attgatccac gatttttaga
    14521 cttggatttt gatccagata atcagtattc gattccatat ttttggggaa ctttagggat
    14581 cgtatataac gataaattct ttgatgcaaa tcaaattaag cattgggatg atttgtggaa
    14641 gccggaatta aaagacagtt tgatgctgat tgacggtgca cgagaagtta tggggctttc
    14701 tttgaatagt ttaggctatt ctttgaatag taagaatatg gcacagttga cagaagcagc
    14761 aaataaattg aatcatttaa caccaaatgt caaagcaatc gtggcagatg agatcaaaat
    14821 gtatatgatc aatgaagaag cctccgtggc agtgacattt tctggcgaag ctgccgatat
    14881 gatgtgggaa aatgaacatt tacattatgt gatcccacca gaaggatcca atctttggtt
    14941 tgataatatc gttatgccaa aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa
    15001 ctttatgcta gaaccggaaa atgcggcaca aaatgcagag tatattggtt attctacacc
    15061 aaatgaagaa gcaaaagcgt tattgccaaa agacatttcg tcggacgaac aattctatcc
    15121 atctgatgac acgatctccc atcttgaagt ctacaaagat ctaggatcga aatacctagg
    15181 catctataat gacctattct tagaattcaa gatgtatagg agataatggc aaaactcagt
    15241 aagaatgggg acttatcaac gttctaaaga gctaaaaatc gtcctttttc tatctgagtt
    15301 ttccaatctt ctaccaaaag cggatgaaac gccgcttttt ttgtgttatt ttattttttc
    15361 taccacgttt ttctaccaga gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg
    15421 ggctagtaaa tctaagattg aaaaagttac agaaggataa agtaattgga aactatgcaa
    15481 atatacttaa cccttctgta atctattaat aaaactaata tatgaatcca gttcaatatt
    15541 tgtagctatg aaaaaacata aaaatcatat tagataaaaa ctaaattggt agataaatta
    15601 ttttgtcaga tatctgaggc aaatattcga ctatttgtgt ttcaaagtaa aaattggaat
    15661 acaaatatag aatagacata ttgcagccga tagtttatta tgcttttaga cacattaaac
    15721 tggaggaaaa aatgaaaaag agcgctaaag atcgagtttt tgatgagctg atacgactaa
    15781 cagggaatat gtcttctgtc actgccgaga aaatttcgaa gaatctaggt atctctcgtc
    15841 agaatgccag ccattatttg acgagattag tggaagataa aaaggtcgag aaattaccgg
    15901 gaaagcctgt tttgtggaag cctttagatg aatatgcggt tgtagataat acggagcaaa
    15961 taaacgaagc ctttcattct gtagttggtc atgacggaag tctgagagag gtcattcaaa
    16021 aatgtatagc cgctgtcaaa tatccgccta acggactcag tgtattaata aatggagcca
    16081 cgggtgttgg aaaaagtttt ttagccacaa aaatttttga atacgctatc catgagcaaa
    16141 ttattgaaaa agatgcacca tttgctatct taaactgtgc cgattatgcg gacaatcccg
    16201 aactgctttc ggcaacactg tttggataca agaagggggc ctttacgggg gctgaaaagg
    16261 ataccgatgg tttattggca acggccaaca acggctatct ttttctcgat gaggtccatc
    16321 gcctatcgaa agaaaatcaa gaaaagctct ttctatttat tgatactgga aactatcgtc
    16381 cagtcggtga aaatgtaaat tggcattctg ctaaggttcg ttttattttc gccacgacgg
    16441 aaaaaggtga aaattattta ttagacacct ttgatcggcg tattcaaatt agtgttatgt
    16501 tgccaacttt tgaagatcgc cccattcgag aacgattaga gctgattcaa ttattttttc
    16561 aaaatgaagc ggatgtttta caaaaagata ttattgtttc aagagaagcc ttgtctttaa
    16621 tgttatctca tccctttagt gggaatattg gtaagctgaa gaatatcatt aaaatcagtt
    16681 gtgcggatgt gtatagtcga acacaagaag aaccattaac gattgggaaa aatgagatct
    16741 tgattcagtt aaatatgctg gaatccgaag tagaaagctt gccgattgct agtttattga
    16801 taaagaaaaa tcaaaagata aaaacggatc gaatcaaacc gtttgattat gattcgattt
    16861 tggaaaaaat tactcagctg ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa
    16921 agaatgaagt acagcatttg gtgcatcaca ttgataagga ttctttttta tcaatgagta
    16981 gaaaattgat gattcagatg tttagaaagg tctgggtatc cgttttagga aaaacgtacg
    17041 gattgacgac gagtggacct gttgcagaaa ttattagcag atcctatgtc atttccccaa
    17101 gctataagac agacttaacc gggataaatc gtacattagc tgcaaatcta cctcggacga
    17161 cttacttatg taatcagttc ttggagcatt taccaccgct ctcagaaaag gacgagaact
    17221 ttctaagact gcttattgtc gtgactttat ctgattatgt agatgaatcc atcgagctga
    17281 agggcctgtt gcttgctcac ggagatagta cggctagcag cattcaagca gtagtgaatc
    17341 aattatgcgg caattatgtt tttgaagcgt tggatatgcc agtagagagt aatgtgtcag
    17401 agattgtcga atcgacaagg aatttcgtga aacggcaagt gaatacagat agtctagtgc
    17461 tgatcgtaga tatgggctct ttaaatcaaa tttacacaca gatcaagaac gatttaaaag
    17521 gggagttact tcttctgaat aatttgacga cttccattgc acttgatatt ggcttgaaga
    17581 tgacgatgaa tgcgccattt aaacagattg cggaggatgc agcagacaag tattcaatca
    17641 atgttcaata ctttgaaggc ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg
    17701 gtttggggat ttcggacaaa ttgaaagatg tttttcgtca tcatttatcg gatgaatcca
    17761 tcgaagtttt cacgaaggac tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa
    17821 cttattttga caagacaaaa ttggtcatca cgacttcgga tcttccacgt tccttttcaa
    17881 ttcctaatat taatgtttat gacatcttag acggtgatgg agcagccaat ttggagcata
    17941 ttttgagtcc tgaaatgaag ccggcagaat ttaaccgctt gattcaggag ctagtaaaat
    18001 tcttttcgtt ggaaggaatt gcggaccgtt tgaactttct gaacccaacg atcgtgatga
    18061 gtgaagtgga aacagtcatt tctaaatatg aaaattacta tcatttcact ttaaatggcc
    18121 gtgtaaaact aaatctctat atgcatacgg cattgatgat ggaacgttta ttcttggctc
    18181 ggacagaaaa atctgaagaa ccgaattatg atttgaaacc agatgaagat gaattttatt
    18241 atgtttctag aagtattttt caatcaatgg agatgaaata taattttaaa gtcaatgctt
    18301 atgagctttc gctgctctat gaattattag gaccgtatat tcaaaaataa agtgtctaaa
    18361 aaagtgtcta atctgttttt tttaggttag acactttttt taatgtgaat taacgtgatt
    18421 aaaccaatgt ttttaaaagt tggcacagga attgcttata taaaagtgaa cagaaagaaa
    18481 ggaattaacc atatgaagaa aattctaata gcgactcacg gtcacttagc ttcaggcttt
    18541 cttagcagta ttcaattact gacggggaag gttcaagaga ttacggtgat taatgcttat
    18601 gtggatgagt gtgattttga acaggagttg aatgtatttg taaaccaggt gaacgagcag
    18661 gatcagattt ttgtttttac cgatttattt ggcggaagtg tcaatcagaa gattaccaaa
    18721 acctttttgg agaaagagat tgctgtaact attttgtctg gctttaatct gccaattctg
    18781 ctcgaaatag tattggcaac caaggaatta acaaaggagg atattcagga actggtaaat
    18841 aaatgccaac aggaattaaa gatgaccatt cttgatgagg tacatgtagt aaatgaagag
    18901 gaagattttt tttaggagga agaaaaatga ttaaagcagt gcgaattgat gaacgattaa
    18961 ttcatggaca agtagcgatg acttggacaa agagtctaaa tttaaccggg ttagtagtag
    19021 caagcgatga ggcggctacc aacgatattc aaaagatgac attgaagatg gctgcaccat
    19081 cgaatgtcaa agtcattatt aaaacagttg atgacgcgat tcatttgtta aaagatccgc
    19141 gggcgaagga catgcgcttg atggttttag tccctaatgt caaagatgcg gtaaaagttg
    19201 cgaaagcttt cccaacggaa atcgaattaa tgaacgtggg gaatgctggg aaaatgacac
    19261 cgccatcgga agataagaaa accttatcaa aggaagtgat gttaacgacg tcagaaattc
    19321 aagacttaaa agaattagtt gaactttatc cagataccgt ttttcagcct acaccggcga
    19381 tggaaaaacg ttcagctatc tctattttga gtgattttta gaaaaaggaa ggagaaaaat
    19441 tatgtatgat gcatttgtag tcgctttggc cgtttttatt ggaattgctg gacatgaaat
    19501 gttcgggatg tccatgctga gtcgtccgat tgttgttgct ccgttagcag gagcgttgat
    19561 gggggatttg cagacggggt tgattgtcgg ggcgtcacta gaagccatct ttatgggtgt
    19621 cgtcaatatc ggaatctcta gtacggcgga gccttctttg gctgctgggc tggctacagc
    19681 gttcgcaatc aaaatgggag gaaacgtgga tgcgattatt cccattgtct ttccactagc
    19741 agtattagga ttacagttaa tgaatatgat tttttctttt gtatgtggac cgatggctgc
    19801 taaatttttc aaatttgcaa aatctggtga tcaaaaagga atcatcaatc tgcacttctt
    19861 tatgtggttt gtccactatg cgctttatgc tcttattcca ttctttgccg ttttatttgg
    19921 agcagatgtt gtcaaaagcg tcttggataa tattccagag gtcatcatga acggattaac
    19981 agtcgctgga aatctgctgc cggcagttgg gatggcgatg ctgttacgga tgctttggag
    20041 taaaaatatt gctgtgtggt ttttctttgg ggccgttgtt atggcgtatc tcgagttacc
    20101 attgattgca attgccgcag ttggaacgat tattgcaatt gttatagccc aaagagatct
    20161 acaactaaaa aatatatctg ttcatgcagt tcaaagcgat gctgctgtaa atgcaacgct
    20221 tgaccaagaa gaggaggatt tctttcaatg aaattagctg atggaatttc taaagatgag
    20281 aaaaaattgg ttcggaagat gttttggcgc tcgatgacta tgtatgcttc ggtgaatccg
    20341 gtgacgatgg gcggcggtgg tttcgcctat tctatgatgc ccttcattaa tcatttctac
    20401 aaaagtgaag aagatcgagc tgcggcgctg gaacgtcata cacagtattt ttcgacgacc
    20461 attccttgcg cgtcctttgt catggggatt gcggcatcga tggagaagga aaattctgaa
    20521 aaagctggtt ttgacgtgga aagtatcaac gcgatcaaaa cgagtttgat gggaccattg
    20581 gctggaattg gcgattcatt gttctgggga gtttggcgtg tcgttaccgc gggactagcg
    20641 atcaacttag ccaatgccgg aaatattttg gcaccaatta tctttttagt tatgttcaat
    20701 atacctaatt acttgatgcg atactatggc ggcgttttag gctattcatt aggctctaaa
    20761 tatattgaaa agctgtatgc gaacgggtta attgaaattc tgacaaaagc tgctagtatc
    20821 ttgggtctga tcatggtagg tgcaatgacg atcagtatgg tcaaattcaa taccgttttg
    20881 agcttttcaa tgaagggcga agaactaatg aatgtccaag aaatcctcga tagtattttt
    20941 aaaggaattg taccgttatt ggtaacattg agctgtttca aattactaga taaaaaagtc
    21001 ggcgtcatca aaattatttt tggaatggtc gtcttgggaa ttttattgtc attacttgga
    21061 atcgcatagt aggaagctgc agctactagg ctgcagtttt tttagcaagg agggaaaaga
    21121 tgactattaa tgaagtaatt tattcaccca aacacccatt ttttaatgct gagttaaacg
    21181 acggaaatgt gactacttca aacgaataca gctactatcg gaatattcct gatgtaaagg
    21241 gattgacctt cgatgtaagt aatcaaaaga cattagcaaa ccttgatgtt tgcgggacga
    21301 ttcaaacgtt gaccttttat cgagaaaatt tactaactga ggaaaaacca ggtgtttggg
    21361 tcaataaaca gctctctcaa accaattcgt tatccttaaa ggttgaagta aacgggattg
    21421 tctaccattt gtcagaatcg gatcatcgta ttgaagtcga tgtgattcaa gactgtttgc
    21481 ctagatatat tcatcattat tcgacgttta aggtcattgt agtatccttt gctcccatac
    21541 tggctaataa gcgtttaagt atgcttgttc aacaggttta cattgtgaat gaaacacacg
    21601 aaagccttca agtttcagtg aaagatgtgc cttcgtatca agagaagtat tcggatcagc
    21661 aaaatgtgtt gatttctcaa aaaggaaata aggacacgat cgcacaacag gaagtggcaa
    21721 gcttcgccat tgcttttgtg gacccaaatg cttttgaaga agtaatgact tttcaggaat
    21781 ctgatatcga aaaatggctg aaggaaacat tgaactattt tgctcagcta tttgggcagc
    21841 tgtcaatgcc aaacgataga gttgtccatc tctatcgtcg tgcactttat caaagctttt
    21901 ctagtttcgg tatgaatcga cagggacagg tcgtcggcag caattggggc agctatcctg
    21961 ctactaatcg aatttggaat aaggatatgt attacgcatc attgccattc accatgtttg
    22021 aaccggagtt atgtcaaaaa acggttctat ggtttgatca atatggcgtt aagtttcctg
    22081 gttcaaaatt tacaggaggt atcaatcatt ctctgagtaa ttcgttatcc agtgcgttac
    22141 tggctagtct ttattttgaa catactgggg atacaaaatt ctttgagcgg tgtccgcagg
    22201 ttctactgaa tgcttcaaga attattgagg acatattagc tcaacgtcgg tcaggggaac
    22261 caatgctttt tccatctgtt tggttatcag acgcctttgc acttggaaag tatcataccg
    22321 gcagcaatct ttgtctttgg aaggcctgta ttggacttgc agaaattttt gaagccctag
    22381 aacaactgag tcttagcaag cgatacaaaa acattgcctg taaactaaag gaatcaattt
    22441 gtaaacaaat gactatcgat ggtccttttg gtgagcaatt tttggaggga atcggtgatg
    22501 aggagaaaac aacctattca gtgaagcact atcaaaaacc gattcttgag caagggttaa
    22561 tctttttaag tgatgtgatt gtggatggaa aaattaattt attgatgcat gatggagaag
    22621 aatcagacac tacgttgatg cctttttatc aatttttaga taataaagat catttatatc
    22681 agaacaccat ggccttttct gctagttctt tcaatccaac ctatagcgag gaaatcaaag
    22741 gcatcacttg gggattagaa tccggcgcca cttttcctgg ctttatcact gttttaatga
    22801 gcgagctgca caaccctcaa gctttcgtaa cccgattaga agaactaatt tgtttggcag
    22861 atttagacgg ttcgtggtgg tggtggccgt atcgcttaga ggctcaacaa ggagaagtcg
    22921 tgcgagactt tggctgcggc aaatgcggct gggcatcggg tctttttgtc agcctaatga
    22981 tcagtcaata tttcggattg aaattcaaaa aaggagtatt gcaaattgat cctgttgata
    23041 atttaacgtt ttcttggaag aatttgaagt tcggtcaagc cttcatttcg attgaatgta
    23101 cgcaatccga gttgagtatt tcaaatttgt cagaaaagcc attgaaaata agtgatacga
    23161 aaaaaattct tcacgtagaa aagggaaaaa cgattcacat tcaaaggaga aaaagatgag
    23221 tatactaaaa aatattaccc aatttaaaaa aatccagagt cgagcaatca ctcccgaaaa
    23281 tccaacaggc gagaaaggta aagctgcgat gttgccaagc gagctaggac caagccgcaa
    23341 aggtcgtggc gcaattccgc tgacttctgg tgaagaaact gtgattgcgg atattactgg
    23401 aacgggaata attcgtcata tgtggatgac gattcgtgat cgaacagaat ttggcagttt
    23461 tgttttacgt gatttaatct taagaattta ttgggatggg tcggatagcc ctgctgttga
    23521 aagtccttta ggggatttct tttgtaatgg gtttggagag cgttgcgagg tgaactcgtt
    23581 gccgatagtg gttaatccta cagggggctt taattcctat ttcgaaatgc ctttccaaac
    23641 atccgctaaa attacgatcg agaatttaca tccgaaggat atgtcctcat ttttttatac
    23701 gataaattac gctttggtgg acgaattgga agaaaatagc ctatattttc acgcaaattg
    23761 gcatcgggaa cgcattacca aattacagga ggattataag ttaattgatc gattaaaggg
    23821 tcaaggctat tacgtgggta cttaccttgc gcttacctgc ctagaacgtt attggtgggg
    23881 cgaaggcgag tttaaattct acattgatgg ggatagtgag tatccgaccg taacctcaac
    23941 aggttcagaa gattattttg gcggggcgtg ggcctttcat gagtacgatg agttcggacg
    24001 tccacatgcg aaaaactatt caacagcctt tttagggtat ccttaccaat caaatagaga
    24061 tgccacaaga gatcgttttg aaacgggaaa attgaatgcc gttcatgcct ttggtgacga
    24121 cgcaatgcca cgacatggtc tttatcgttg gcatcttcaa gaccccatct gttttaccga
    24181 agatttatcg gtcactttac aacaaatcgg caacgatgat gttcgcttat ttgagcggca
    24241 agacgatgtt gctagtgtcg cttactggta ttcaacagaa gcttcaggaa atactaaacg
    24301 tcatttcact gatcgagaac gcttgccgcg ttaatgaagt gtttttcaca tattcgaagg
    24361 gaggaaccta tgatgatata tggggcaatt gaagctggag ggactaaatt tgtctgtgca
    24421 gtcggtactg aggaattaac gattttggaa agagtcagtt ttcccacaac aactccagtt
    24481 gaaacaatga agcaggtcat tgattttttt aaacctttta aggaacaatt ggcaggcatc
    24541 ggggtcggct gctttggtcc aattgatatt caatgcaaat caacgagttt cggtcatatc
    24601 acatcaacac caaaacttgc ttggcaaaat ttcaattttg taggaacgct aaagcaatat
    24661 ttcaatattc cgattgaatg gacaacagat gtcaatgcgg cttgctatgg tgaatatgtt
    24721 gctggttctg gcaaagggtt agcaggtgtt gcatacttca ctgtgggaac aggtatcgga
    24781 ggtggtgcct tgttcaatgg gactttcgtg gaagggttca gtcatccgga aatggggcat
    24841 atgttgatca agcgacatcc aaaggacaat ttttcaggaa actgtccctt ccaccaagat
    24901 tgtttagagg ggatggcagc aggaccagcg attgaaaaac ggttgggtgt aaaaggccag
    24961 aatttattgg ctgacgattc gttttggcag attgaagcct tttaccttgc ccaatgtgca
    25021 tacaatacaa cactgatgtt ttcgccagat agaattattt ttggcggtgg tgtaatgaaa
    25081 caagaacaca tgaagaaaaa agttcaggat aaatttgttg agcttataaa tggatatgtt
    25141 gaaataccac caattgatag ctacattatt acgcccgaat taggtgataa tgccggaata
    25201 atcggcggat tagcattggc cagaaaagcg gtaagaaata agcaaccata attaatgaat
    25261 gtttcattga tactcatgtc aaaagtattt aaccgagtaa tcaaatgatc aaatgaagtt
    25321 ggataaagcg aaaatgaatt caaataaaaa ggctctaagt caaatcgtgt tgttttcatt
    25381 aaatcactga aaggaaatgc ttctgcgttt ccttttttct ttttgttgtg cgctgtatac
    25441 cttgaggata ttcactggtg gcgtacactt aaatagtaat atattgtgtt actttatgtc
    25501 tgtggtaata gttatagttg cttaccaatg tatgttcatt tttcgaaatg ttgacttcat
    25561 aatccttggc ttaatgtttg aatttttttg gtgatagatt ttttcagccg tataaaataa
    25621 atcgtaattc tattatagag agttgtgatt ataaaaagat agcaagaaaa aaaaagaaag
    25681 tttctataca ttttaaatat tttattccta tttctattgc taaaaaagta gaaattctat
    25741 atttaccttt aactctcgct tctcttgcaa atgtagttag cattttaatc gataa
//
LOCUS       DARBER010000024        24938 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARBER010000024 DARBER010000000
VERSION     DARBER010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.024"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1122)
                     /locus_tag="SCK06_001032"
     CDS             complement(<1..1122)
                     /locus_tag="SCK06_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151052.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLF"
     gene            complement(1109..2977)
                     /locus_tag="SCK06_001033"
     CDS             complement(1109..2977)
                     /locus_tag="SCK06_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151053.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            2943..4580
                     /locus_tag="SCK06_001034"
     CDS             2943..4580
                     /locus_tag="SCK06_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151054.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(4725..5465)
                     /gene="truA"
                     /locus_tag="SCK06_001035"
     CDS             complement(4725..5465)
                     /gene="truA"
                     /locus_tag="SCK06_001035"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1151055.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(5656..6291)
                     /locus_tag="SCK06_001036"
     CDS             complement(5656..6291)
                     /locus_tag="SCK06_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1151056.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(6438..7679)
                     /gene="sstT"
                     /locus_tag="SCK06_001037"
     CDS             complement(6438..7679)
                     /gene="sstT"
                     /locus_tag="SCK06_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG1151057.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            7877..8875
                     /locus_tag="SCK06_001038"
     CDS             7877..8875
                     /locus_tag="SCK06_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151058.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8888..10906
                     /locus_tag="SCK06_001039"
     CDS             8888..10906
                     /locus_tag="SCK06_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG1151059.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(10966..11682)
                     /locus_tag="SCK06_001040"
     CDS             complement(10966..11682)
                     /locus_tag="SCK06_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG1151060.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(11909..12451)
                     /locus_tag="SCK06_001041"
     CDS             complement(11909..12451)
                     /locus_tag="SCK06_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1151061.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12456..12683)
                     /locus_tag="SCK06_001042"
     CDS             complement(12456..12683)
                     /locus_tag="SCK06_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1151062.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(12762..14642)
                     /locus_tag="SCK06_001043"
     CDS             complement(12762..14642)
                     /locus_tag="SCK06_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1151063.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(14831..15445)
                     /locus_tag="SCK06_001044"
     CDS             complement(14831..15445)
                     /locus_tag="SCK06_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151064.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            15587..16147
                     /locus_tag="SCK06_001045"
     CDS             15587..16147
                     /locus_tag="SCK06_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1151065.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(16452..16766)
                     /locus_tag="SCK06_001046"
                     /pseudo
     CDS             complement(16452..16766)
                     /locus_tag="SCK06_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(17020..18655)
                     /locus_tag="SCK06_001047"
                     /pseudo
     CDS             complement(17020..18655)
                     /locus_tag="SCK06_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19210..19773)
                     /locus_tag="SCK06_001048"
     CDS             complement(19210..19773)
                     /locus_tag="SCK06_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1151066.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(19788..20831)
                     /locus_tag="SCK06_001049"
     CDS             complement(19788..20831)
                     /locus_tag="SCK06_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151067.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(20964..21368)
                     /locus_tag="SCK06_001050"
     CDS             complement(20964..21368)
                     /locus_tag="SCK06_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1151068.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(21601..22236)
                     /locus_tag="SCK06_001051"
     CDS             complement(21601..22236)
                     /locus_tag="SCK06_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1151069.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(22502..23554)
                     /locus_tag="SCK06_001052"
     CDS             complement(22502..23554)
                     /locus_tag="SCK06_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1151070.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(23567..24505)
                     /locus_tag="SCK06_001053"
     CDS             complement(23567..24505)
                     /locus_tag="SCK06_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1151071.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         7989 a         4802 c         3899 g         8248 t
ORIGIN      
        1 aaataaataa aatagttctc ttgtcttatt gtatcttcta tttttgaaaa atatcatcgt
       61 taatatcaga gaagagaata ttatttgcaa gcttaagatc cgccaaggaa attgtaacaa
      121 tttggctggc gtattttgca gaagatacca tggcaccaaa ctagttgtag ctagccaagt
      181 caaacacgta ccataataaa tctttttttc tatgtttgtt aattttttaa ataaaatcag
      241 gggaatacat aaagataaca gaccaactag tcccattgag taagtagtta agtcgttgtt
      301 catagcttca gtcagactat cgaacacatt agatgcatga gcatataaat ttgtcacgct
      361 aggtttgttg attttttggt agagcatttg ttcaaacatc ggataccaaa aagcactcgt
      421 cagaaccaat gtcattcctg ccgcactaaa taatttttta aatatagcaa taaaatctgc
      481 attcttgaaa cctttagaaa ccaaacgaat aaaaataaaa atgataatta atgctgtact
      541 cattaatgtt gatagtacat ggctgtacac tagcaaactc atactcaatg ctagctttac
      601 agacggatac ttttcttctt ttccagagat gatttgataa gcataaaata atacagggat
      661 aacaagtgtt attgcgatag cttcggcaat tgcggaacga tagtagatgt ctactgtccg
      721 ataagcagag aaaatataga gacaactaaa taaaaatgcc gctttttttt gttttaaaaa
      781 ccgctcgcca taatgataag ctacgagtgt tgtacacaag ttcaataacc aaacgtaaat
      841 aatatagctt acaatcaaat tttttgtcat ccaatataac attacagctg gaaaaaaagt
      901 cagaaaggga taaaaataat ttacaccata tccagtataa ttgaatgtct cataattaat
      961 cggactggta aaaatagtcg ataacccctt gattcgagat aagtgaaaag aaagatctcc
     1021 agtggtataa atactctgtg tatttttgaa aaagatgaaa gaatatatac tactaactat
     1081 aattaatcca ctcaatatta ctactttttt atttattttc attcttcttg tttatctcct
     1141 ttttagtcac atatcgagaa tctttcaact tattaggtgc aaatcgttta aaacttgaat
     1201 acaccactaa acaataagtc agaatactga tccataaagc catgagagca gttggagtat
     1261 attttgttgt aatttctatc ttattttctc cagcatttac atccacattc actaagccat
     1321 gttttttata agagtcattt acgactttac cgtttacttt tacctgtgta cctaaataag
     1381 catatactgg tactgtcact ttttgctttt tgttgcttgc cactgtgtaa gtaatgttat
     1441 tatctgcagc tttatacttc cctttttccc acgattctcc tacttttact tcatgttttt
     1501 ctaagtgagg cctttctttt atcgcttctg ttggggcgta atcgtataat cctcccatat
     1561 ctgatgttgt ataacgtggg agtgtttctt cattgataac aatatgaccg tgttttgatt
     1621 ggataacttt tgcgtaattt tcttttgctg ctatagacgt gacgagtaga aacaccacag
     1681 tagcgccaat gattaaccat gaatattttt tattcatagt ctttttcata aaaacaagcg
     1741 ttatgatcat tgaagaaaaa aagatttgga tacctaagat acgccaagga aactggagta
     1801 agcttatcgg tgttttttga aacaaaaacc atggaaataa attagttacg catacccatg
     1861 tagaaactac gccaacatag atgatttttt cttttttctc catttctttc agtatgaata
     1921 aaggaaaaat caacgcaacc aatcctaata accccatagt gtatgttgta atatcgttat
     1981 ttaacgcttg aatgatacta tctgaaatag aaagtgcttc tagctgcaaa tctgttttga
     2041 agggactgtg tatttcttga taattcatct gctgcaacat aggataccaa aaatagctag
     2101 ttaatgcaca agtcaaaaaa acagccttta ctaaattcac gaccacaaat tttatatcgt
     2161 tttttatttg cttgcttaaa aacaaacgaa ctataatcat acaagcaatc aatgcactac
     2221 acaatagtgt agttaagaca tgagtataca caagtagact tatactgaca gataaagcga
     2281 tgactttttt ttctctattg aatatcaatc cataagcata atataaaaca gctgggataa
     2341 tcgtaagagc gattgcttct gcaattgcgg atcgataata gatatcaact gttcgataag
     2401 ttgcaaaagt atataaacaa ctaaataaaa aagcagcatc tatcttttta aaaaactttt
     2461 ctccataata aaaactaatc agaatcgtac aaatatttaa taaccaaata tatagaacat
     2521 aacttaatat taagttattt gaaattccat aaaaaacgac tgcaggaatt attgtaagaa
     2581 aaggataaaa atagttcagt ccatctccat aattgttaaa tgttgtatag ttgattggcc
     2641 cttcaaaaat agaagataac cctttgattc gtgaaagatg aaaagataaa tcacctgttg
     2701 tataaatttc ttgagcattt ctcataaaat ttactgaata taaagtacta agtaagataa
     2761 gaactactaa aagaaatgct tttttactta ttttcataga tttgttgttc tcctttttta
     2821 attctatttt cagtatacta gaattgtctt tttttaataa gttttgcgtt aaatttttca
     2881 tagcactagt atgatatact agaaaagaat caatttttat tacttttaaa taggagaaaa
     2941 aaatgaaaaa aaacaaaaac caaataatag atatcatttt tatgttattt ctaagttttg
     3001 cttacgtcat ccctctattc atgtctaagg gaatctatca tagtgtcaat caggatactt
     3061 actttcattt atctcgaatc ataggactag ataatgtatg gagttctcca gtaaacttca
     3121 ataacttcga tcatcatgga acaatgatga atatttttta tccatggctt actctttatc
     3181 cagctttttt attttataaa atgatgggaa atcttgtttt aagttataat atctactatt
     3241 tttttatcac ttttttaaca atggtcgttt cttatttttc tatgaaacaa ataaaaaaca
     3301 atcgttatat ttcgctgtta ttttcgatta tttatacatt ttccgcctat cgtgccattg
     3361 acatattcca tcgtgcttca ttgggagaag ctgtcgcttt aacatttttg cctttgatac
     3421 taatggggtg ttacgaaatt tatattagag attaccaaaa atggtactgg ttgtctattg
     3481 gcatgacgct agtagtgtat actcatttac tttctgtcgc tatggtttct gtatttattg
     3541 gaggaacgtt gtttctttct ttttattttt gggaccaaaa aatcgctaga ctcttaagtc
     3601 ttttaaaagc taccgtcctc accttttttt taagtgcggg attcttaatt ccttttattc
     3661 aacaatctag agcacaagaa ttgaaagtcc cactgggaaa agaattaagt ggtatggctc
     3721 ctagtgatat gctgacacat attttaaata ataactataa caactatact attggattat
     3781 tcttatttct cggattgatt ggtgctttta tttttataaa aaaattaact gctgatgatt
     3841 tatttatttt ctttcttggt gtatttgttc ttttttgttc aaccaattta tttccttggc
     3901 aactttttaa ccatacacca gttaagagtc tacaatttgt ctggcggtta aatggttttt
     3961 cttctttatt tatagcctat acgatgagta tagtgatcta ttatattttt tctactaaaa
     4021 ataattcatg gaaaataatg gtctttacgt ttcttgcact aatattgcat ctctcaggag
     4081 taagcaattc tatccatgct aacaaagatt cattaacact tatagcacct gaagacgctg
     4141 ttgctatagc tgaaaattac aatcatactg attatgcaaa caaagagtca atctatcatc
     4201 ctgatatgat taataatgat caatatttac taggtaacca ggaaataaat ccaacaactc
     4261 attttatgag taacaaactt acaattgaat tagataattc gaataataaa aatactgtct
     4321 tgacgacacc tatttatcgt tataaagggc aagtggcttc tattaatggc aaactagtac
     4381 aaacaaaatt atctaagttt ggcacaactg aattaactat tcctccaggt ataaataaag
     4441 tcgttattac ttatcagtac acaaaactag caattgcttc aagatattta tctatcgtta
     4501 ctttgatttt atttttactt tatcgattta ctttttctaa acaaaaacaa ccgagaagag
     4561 aagtacaaca ttctcactag atttttattt aagaaagact ttttagctgt acacataaac
     4621 aggctaaaaa gtcttttttt atatttcttt aatttgcttc atacattatt taacatcgat
     4681 atttatactg tgaaacatgc gtttcatcca ttatcaaagt atttttatcg ataataaact
     4741 tcatccaaaa acagaccttg tgcagggacg gtttctcctg catgttgccg cactttgctt
     4801 tcaaatattt catcgattgt ttctatcggc atggcacctg tgccgatttc tagtaatgtc
     4861 cccatgataa tccgcaccat tttatataag aatccgtctc cgacaaaagt aaaatgaagc
     4921 atttctccct ttcgttcgat agataattca tcaatcgtac gcactgtgga tttttttgat
     4981 tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca ctaatttttc gcaagctgca
     5041 ttcagcttgt ccatatccaa ctcttgaggg aaataaaagc tgtggtttcg ctcaaatgct
     5101 gaaggaatca cgttgttcca aacataatag ctgtattttt tgcccaccgc attatatctt
     5161 gaatggaagc gctcaggcat ttcttcgatt cttttgatga caatatcccg cggaaggtaa
     5221 cgattcataa agtcgatcat ttctgccagt ccaatttctg tgtttgtttt aaaatttgct
     5281 acttgtcctc gcgcatgggt gccagcatct gttcttcctg atccaataat ttctatctct
     5341 tcatttgtca tctgatgcaa tactttttcg atttttcctt gaatggtttt atcgtcatct
     5401 ccaagtcttt gccatcctga atatcttttt ccgtcatatt cgattgtcaa cttgatgttt
     5461 ctcatatttt agtggctcct ctgtcttcca tttggtcttc tctctacctt tttaagattg
     5521 tagcacaaaa tatagctcct tgcttctcta agacagaagc tatacaagaa aactgtggaa
     5581 atatatattt cagaaaaaca aaaaacagcc accggatgaa gtatccgctg actgttcaat
     5641 ttgacggctc aagttttact gtttgagatg caagatactc aacctggcaa tgggcacata
     5701 attaaagaaa aaactgattc tttggcaaaa acctatttta ttcagtaatg gccaagaatt
     5761 tatagaagga attcttgcca gtccataaat cgccgcaaaa agcaaactga tcacagacag
     5821 tccaaggtat aagtggctta cgtccatttg cccatttttt cgatagatca agaaaagaag
     5881 aactggaaac aataaaaaac cactgtaacc taaacctgat ccgatattat ggacccatgt
     5941 cgagacattc cacgaagatt catgggtatt gatactgaac aaacctgtaa agatacaatc
     6001 acctatacca tagaacgcga tggctgctgt aactagggta gctaacacac gagatgtact
     6061 aacaaatgca gcatacaatg caggcaatga caggatgaaa aagatccctg aaaccacaga
     6121 ccaaataaga aacgcttttc ttacaggact ggaaacctct ccaaatacac tgatcacgct
     6181 cttcatttga tctaattcag gataatacaa tcctaatacg tacggagtca aaaaatcact
     6241 cagcacgcct aataataaaa agaaaaaacc aaatttacgt aaaaaatcca tgatacgcct
     6301 ccaataacaa ttacctatgc ttttaattgt tattataaac gactttatct tgatttaaca
     6361 gaaacctcta tttacacgaa catctatata actcattaaa aactcgaatc attttaaaaa
     6421 tactaaatat ctaacgttca acgttcactt aatccatcta aacaatcttt caatttgatt
     6481 ttttccccac ttcttttacg cgcgcctagc tctcctattg cagtaaacaa cagatcactt
     6541 gacgaattta aggcagtttc cattgaatcc tggataacac cgataataaa tccaataccg
     6601 acgatttgca tcgcgacatc attagagata ccaaacaaac tacatgctaa tggaatcaat
     6661 aacagtgaac cgcccgcaac acctgaagct ccacaagcag ctaaagcaga caaaatacat
     6721 aaaaggactt tcaaaacaaa aggtgcttca atccctaaag aatgtacagc agtcaacgtc
     6781 atcaaggaaa tggtaatcgc ggcaccgccc atattgatcg tagcacctaa cgggatcgat
     6841 atggaataag actcttttgt caatttcaat ctttcagcca acatcatgtt tatcgggata
     6901 tttgcggcag aacttcgggt aaagaaagcg ggaagtgcgc tttctttcaa acaaaagaaa
     6961 atcaatggat agggattttc tttaataaac aaaaacgtga ggaatgggta cacaactaat
     7021 gcgacaaaca gcatcgtacc gatcaaaaca agtaaaagct gcagatactc tgccaatcca
     7081 gcgattccag tcgtagcaat tgattgataa accaatccta aaatccctat cggagcaaat
     7141 gcaataatca cttgtacgac ttgtgacaac gctatagata tctgatccac caactctttt
     7201 gtcgattctg aacgtgctcg aaaagctagt cccaacaata cagcccagaa taagacgcca
     7261 aggtaattcg catcaataag agattgaaca ggattttgtg tgacattcgt caacatagta
     7321 gataaaacac tttccaaact ttgcggtgct ttttcagata ctgtttcttg taaagtaata
     7381 ggaacagtaa aaattgaact tacaataact gctacaagtg cagaaagcaa tgttcccact
     7441 aaatataaga tcaaaatagg cttcacgaat gtcttattcc caattttatg cttggcaatt
     7501 gacgccatcg tcaagaaaaa aaccagaata ggtgcgattg ccttcaagca actgacaaac
     7561 aacgttccca atattgaaat aaatgtccat gtggggaaaa agataccaaa caatattccg
     7621 actaaaatac caacaacaat acgttgaact aacgacatat ccaaaaactt ctctaacaaa
     7681 tgaccaccct ttcacaattt taacaatcat tgtatcattt tatgcaaaga aactaaaggt
     7741 ataattttct aaaaattcta acaatacaaa caaccatttc ttgctaattt tgtacttttt
     7801 ctgtctacac gttgtttttt gagatattct aagttaggat gttattaatg ggcaacttat
     7861 caggaaacgt tttcttatgt ttttgtccaa tttttttgtg aggtgtttat tcttgaaaaa
     7921 aataaagaag gagagttatg gcttcctgac aatctttgtc gcttgtataa tgatggtttt
     7981 acagcgtata ttgatttcag cagtcactat tcctacatat gcaacaaacc ctggtcgatt
     8041 ctacatctat acatttttac aaacttccgt tgttattggt gcaaatttga tcccacttta
     8101 catgggttac caatatcaaa aaatcgcccg tttaaaagtt ttacattatt taagtcgttt
     8161 tgcctttata ttcctaatag ccacactagt ttgcaatatc tttttttacg tacaagctgg
     8221 cagcttgaat attcgtgatt attggcttat cctaacacct atcagtcaga attactttac
     8281 ttatgctgtt tcttgtactt tgcttttttg tagtattcct tatacagttg gatttttaaa
     8341 taaattatcg aaaacaacaa ttaaatatct actattattt ttaacaattg gattagttgg
     8401 ctgctctact ctatttaata aagacccgtt tgcattacag aatggacaga gtattttatg
     8461 gattttttat ttatttttaa tgggttatgg tttgaaagaa tggaactgga aagaaaaggt
     8521 ccgctataag cttcctcaag tatttatcag cttgattgtt ttattcggtt tgattatttt
     8581 gatgacacaa atttcactca tcatcagagg aaatacagat acagcgctga ggttttctaa
     8641 accatattct ctcttcgcaa tgtattatac tttctcttcg tttttatttt tagaaatgct
     8701 atctgataaa ttaggtataa aaggaagagg aaattttctt gctgtatttt tgattgtcac
     8761 tcaagttata tgtaatttcc ctttagtttc ttatcgcgta tctacattta tgaaacgtga
     8821 atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta tttgatattt
     8881 attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc gtttcattca
     8941 tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc gttggaaaaa
     9001 aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat ttttttactg
     9061 ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag aaactatcca
     9121 ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa tcatcattat
     9181 tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg ccttgactgc
     9241 taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg aaatgcggga
     9301 agagcctgtc tttccttcag atttaaaaat gctgaccgga ttgtcagaac tattatcgat
     9361 ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat tgattactag
     9421 tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt ggaaacgtcg
     9481 atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca ttaatcacaa
     9541 aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt tctttaacca
     9601 gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata tcgacattaa
     9661 aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa tgaaaaagta
     9721 caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca atcagacgat
     9781 cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc tgacagtacc
     9841 tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag gattgatgtt
     9901 atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga caggcttgag
     9961 tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg agcgtcagaa
    10021 aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc cttttacagc
    10081 tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt tctattattt
    10141 agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac gcatatcgga
    10201 tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacggaaa cgtcacaatt
    10261 catccaatta tccactatgc aaaaccatat gccttatgat gattactata gtgaattgaa
    10321 ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac aaacttatat
    10381 gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa tcgacaaaat
    10441 cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct atagtgggaa
    10501 tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt ataacaacaa
    10561 gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta ataactttgg
    10621 tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt tgttgacaga
    10681 agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt ctaatcgttt
    10741 taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa aaaatttaac
    10801 tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc ttgttgcagg
    10861 taaccagtac agtgcaacat gggcgacaca aaaaataaaa gactaacaaa taaactgagc
    10921 aaggggttgt gataaaagac atgtcacaac cccttgttta tctcattaat ttgcttgtcc
    10981 ataaacgatt tgattaaagc catttttcaa ttcattattc gctgtctcgt tgaagtaaag
    11041 ataattatcg ggattcccct gattttgttg tgtcaccaat acttttggct gattttgctg
    11101 aaacccgaat agatagacgt gctttttcag atatggttct gtctctgcat cggaatagac
    11161 ggctgtgatt tgatagtctg cttgacctgt cccactttct gaccattctg cagaaacagg
    11221 cactccacca atagccatct tccactcccc gttcaaaata gcttgaggga ctttaagacc
    11281 atataagtcc acactcattt gattcgtata acttttgtat tgttgtccca ttgtttggcc
    11341 ccatttggtc ataaatgcct ctaattgtgc ggatttgtct gcattccaca gtgtttgttc
    11401 aacttgttcg ctgctttctg aagaggaaga tgaagcctct acagaagaac ttgaagtaga
    11461 tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat ctgctgtttc
    11521 tgtacttgct gcagttgact tgctctctgt gctagttgtt gtagtagttg caactgttgc
    11581 tttcgctgtt tcttttgttg attctttttt cgattcattc ccgttggtac aaccagctaa
    11641 caaaaaagct agaccaattg ttactaaaat cctttttttc aattaaaaaa ctcctttcga
    11701 ttactcccac ttctttattc tatcaaataa gcaatgtgat tttattgatt tttcgtattt
    11761 tctcatcaat ctttcaaatc ttctaaaaac tgagaagcac ccgctgatgt tgtctgtaat
    11821 acatcagcgg gtgcttcttt tattttaatc ttcgtgattt tccaatttac ctcacgcttt
    11881 atatttgtat ttgaagaaac atagactttt agtcatcatc gtcttcctca tccaaatctt
    11941 gtcggacagt ttttccttgg agtctttgta cttggtagat gatttcttta ttgaaactta
    12001 aaagttcaac aagcaattgt tgcaagccat atttttcttc tttttcagct aatttgattg
    12061 cccgtgtgat aaagacattt tggaaatcaa aatcttttgc aaagttttcc aacattgttg
    12121 aggctggtaa atatttgttt tgaccatgtt cgtaaatttt tccataacgc atgacttctt
    12181 ctaaagtaga cggaacgatt tcattttcaa tcactaactc atgagagatt ttattgaacc
    12241 atctcgcttc ttgatcagca tattctgaaa aaagggtact taattcttgc gaagatgtac
    12301 ccaaaacata caattgtgct tggatcaatt tcatttcttc taatttaaaa tttgctaata
    12361 catgccccac catggcgcca gcagttggtt tatgatggtc gatatctgct tgttttgttt
    12421 cttgaacaaa tttttcttcc actatattca ttgatttacg cctcttttac ttttgctgat
    12481 tggacttcat aacctaattt ttccacaact gcagctaatt gttcagggtg gacttcatct
    12541 ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt gcttacccca
    12601 ggctgttgag aaaccgcatg ttcgattttt tgcatacagc ttggacaagc tagtgtatct
    12661 aattgcataa taattttttt catcgtagtt cttccttcct ttttcttgat agtcttattc
    12721 tagattacag cttccataaa aaaattgatt cttatcaagt tttacgattt ttcttctttg
    12781 ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga taacgatcaa gatactagct
    12841 tcgtgaacga acatcccact agccatgtaa ataaacccgg caaataagcc gttgagtaaa
    12901 aataagacga caccaatggc aatcactaca ttttcacggg tatttaaaac agttttctta
    12961 gccaagcggt aggcatgaac caacgattca aaattagatt gcatcaagac aacgtcactc
    13021 gtttcgatag caacatccgt tcctgatccc atagcaatcc cgatatcagc cgcagccaaa
    13081 gagggagaat cattgattcc atctccaata aaagcaacgc gcaatccttg ttcttggtat
    13141 tttttcacaa attgaacctt ttcgtctggt aataattcag catggacttc atcaatgcct
    13201 aacatatcag ctacataatc agcagtcatc tgattatcac ctgtcagcat cactaactgt
    13261 tttgctcctg cttgtttcaa cttagctaat tgttcagcga cttctggtcg aatcacatct
    13321 gacacaccta tgatttgagt gatttttttg tcgattgcta caataatgac cgagctgcct
    13381 gtttgttgta attgatgtaa atcttgtact tgctttggtg tgagagaaag tttgtgagcg
    13441 gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat caatctcagc tgtgattcct
    13501 tgtcccttga ttgtctgatt atctttgact gctaattgtt gaaaatcaat gtctttctcc
    13561 tgagcataat ggatcacggc gcgccccaga ggatgatcgg ataacgtttc taatcgagcc
    13621 actaaagcta agctgtcccc ttcatcctct gtatagttct tgatagtgga tacagcagta
    13681 gttcctttgg tcaatgtacc agttttatca aagacaaaag tatcgatttt ggaaaaagta
    13741 tccattgctt caccgccttt gactaagatg ccccgtttcg ctccattacc gattcctgct
    13801 acattcgaaa caggcgcacc aatgactaaa gcgccaggac aaccaagaac caaaacagtg
    13861 attgctaaac gaaaatcacg actaatcaga ccaacaacca acgcaataag taaaacggtt
    13921 ggagtataat actgagaaaa tcgatcaatg aatttttccg catgggattt ggtatcttgt
    13981 gcttcttcta ctaattcgat aattttggca aaggtcgtat catctccgac cttagttgct
    14041 tcaatcgtca cgtagccatc tgctaaaatc gttccagaga agacttcatc tccactgatt
    14101 ttgacgactt gtcgtgattc accagtcact gcggcttcat ccgcataccc tttcccgtca
    14161 atgactcgtc cgtccacagg aatagctcca ccagttttta ctaagacgat atcgccttct
    14221 tccacgtcat cgatttccac ttcttcagtc gttccatctt cttggacaac actagcagta
    14281 gatggggcca tctcggtcaa agccttgatt gactcccgcg ttttggtcaa tgttttttgt
    14341 tccagatagc taccaaacaa aaacaagaat gtaacgatag ctgactcctt gtattctccg
    14401 atcataaagg caccaagcac agcaatagta acgagtaaat caattgagat aactttgact
    14461 ttcactgcct gatacgcatg gatcataata ggtatgaccg caataataga agctatggct
    14521 agagcaatgt tatagaacaa gacaaaacca gtaaattttc cgataaaacc aaaaataatc
    14581 aacacagctg ataaggcggt gatatgattt cgatgctctt ggatccactt ttgtaatttc
    14641 atctttctcc ctcttttcct ttcattctta cgagactaag tatagatact tatccatcat
    14701 aaaaaattga ccactatcaa gtttagtcaa aaaggcggtt ttcttttggt agaatacact
    14761 acaaaaaata gttagcaaga agactcacta aaagtgatct tgccaactat tgtcgtttct
    14821 atcgaaattt tcattgaatg attttcctgt acagcataca tcggagatct atttctttat
    14881 ctaagatttt tatcttgcta attttttcct ttagtctttc aaatccacgt cgttcataga
    14941 attgatacgt acagccacta tctgtaaaaa ggaatatttc ttttcctttc tctctttttt
    15001 caaattctgt tagcaacttg gtaccgatgc cttttgtttt caaatcagga tttgcagcta
    15061 aaaaaatgat ctctccatct ggcgaatgct tctttaaata tgctgcaagc atttgttcat
    15121 ttgctgtctc gtaagctcct ttactttctt tagcaaatat attttgtaaa aaatcgaata
    15181 tcttaacata tatttgctgt ccaaaagaac gatgtttctt tttttctcct tttatctccg
    15241 ccagcaaaac acctgccaac tcgtcctctg catacaaagc aagaatttga gttgctcgat
    15301 tcaactccat atacaaaaaa tatcttccgt aaagattcag cagaagttca ttttccatat
    15361 atctctcaaa gttcattcct tcaacagcaa aatagatagc ttttttgtaa tctttctttt
    15421 ctaagggttt aaaagttagt ttcatcattt cgcctcctca acttcaatga ttgtgttata
    15481 cttacattat aatagacaca gtgtctacta tctataatca atcatctata agtttgtcga
    15541 ttaagcgaca gctttgcttt gatatgtcgg ataaggagat aggtaaatga aaaagacaga
    15601 tagacgcgtc aaaaaaacag aaaaagcttt ggctgaaaca ctatccacat tattagtaaa
    15661 taaaaagatt caagcaatca ctatacgcga actaacagaa acggcagatg tacatcgttc
    15721 gactttttat acacattata aggatatcta tgatctatat gatcaattag aaagcaactt
    15781 ttttcgggat ctaaatgaaa ttctctctta tgatccagcc catagctatg aggaactata
    15841 cactcgcttg attgactatg tttacgcaaa tactttttta tatcgcttat ttatcctcaa
    15901 ccatggtgat atttcgtttc agaagaaagt taaacaagtt attgaagaaa actatctgaa
    15961 catatggcta tatgaagaca agaaaacgat tatcacagaa gaaatgagat tctttactac
    16021 gtaccacgtc caaggttgtc tatccgttct caatcggtgg gtagaaagca attacaagta
    16081 ctccaaaaaa gaattattag aactcttaca gaaactgaat gatcacatag agaaaatcat
    16141 gccttgaata aaaacatact aagcttagtt aagaagaacc gtaaccttct cgtataacaa
    16201 ctgaatctta tttagccaaa ttgtttactg aagatttttt gagtaaaatt tctataattc
    16261 ctacttattg aaaagtagtt tgtagtgctg cttctccctc tctaagtgat atatcttgtt
    16321 gtaccaatca aattcacctc atcattaata aattactttt aggctaattt attacaactt
    16381 ataggttgaa gctgtaacca atttatagtc atattatatt tttatcatcc agtttattga
    16441 attcttctcg tttacctcaa ttcattttca aggggaatca gctcatcatc taacatttta
    16501 cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg atgtttggct
    16561 ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat tggatcttca
    16621 cctggcatat attgcagatt agaagcatgt aattttttct cagaaacaaa ccactcattg
    16681 gaaaagacat gaatcaaatc atcacttact tgtgtaaaat agcgacgttt aatgacaaag
    16741 atatcttcat tgctatctag tgtaccttca tttctttgtg cttaaattca aagataattt
    16801 tctcaataaa gatttttgat tccagtaaga ttcctttact ccttttacaa catagcgttg
    16861 tcctttgttt tgaaacatct tcttcccctc tttgccatta aaaatagccc aattctctat
    16921 aaagaagatt aagcttcatt attataatat aaagtttaaa tttggtttat tacttacttt
    16981 ttcttattct ctagtaagct aatattgaaa aaaatgtaat cagatcatta tttttatatg
    17041 ttgtaacttc ctgataagaa aactaattaa aagaaagagg taactacata aactaattaa
    17101 aaaagctaac ttcatagttg gaggataaat ggaagaaact tcaatgattg tttctccttg
    17161 tgttccttca atcgacacta gtcctattat caatctctct tttttgtcca tttacttttg
    17221 cgactgttcc ccaataatca aatacaggaa gcgttatttt ttgtccttta gattcatgta
    17281 ctgtatagac tattttcgaa tccatcactt gataaggcat agtatgccat tccttgccaa
    17341 ttcttacttc atgattctgc aaatgttctt tgtattgtaa tgcttccgtg ggtgcatagt
    17401 cataaagtcc tccgggaagg agtgtcgtgt aacgaataag attttcttga ttcattacca
    17461 agtgatctct ctgagtgaca actttatgtt catagtttgc ttttgttgca gcagatacag
    17521 ccaacagtaa taaaacaatt gccgttatat taactacttt tctttgctta gctgatgtgc
    17581 tcttcatgaa aacgaacgat aaaatcaacg agccaaacag tacttgaaag cctaagattc
    17641 gccaaggaaa ttgaatcatt tgaatgggtg tattttgtaa taaagaccat ggaaaaaatt
    17701 tggtggataa gagccaagag actaccgcac taaggtaaat cactttttct ttgcgattaa
    17761 atcctttaaa aaagaagagt ggacagatca aagaaataat accaatgatt cctaccgtaa
    17821 aggttgtcag gtcattattc atcgcaccaa tcaaactatc cgagacattt aatgcttggt
    17881 gttgtaaggt cgttttatct ggctcattaa tcgattgaac catgatttgt cggaacatcg
    17941 gataccaaaa gtatgctgtt aaaagaatcg tcattcctac tgcctttgac aactccacaa
    18001 tcaaccggat gctttctttt tttcttgttt cacttactac aaagccgcct aagactagta
    18061 agccaacggc tattacactc ataaaagtgg aaagaacatg tgtataaatg agtaagctca
    18121 tactgactgc taatggaatt gcacatggct ttttctcata aatcagtgcg taagcataat
    18181 acataactat cggtataacc gtaagtgcaa tcgcttcagc aatagcagaa cgatgataaa
    18241 tatcaatcgt acggtaccca taaaatgtat ataaacagct aaatataaaa gccgcatcga
    18301 ttctttttaa aaattttagt ccataatgaa acatcagcat gatagtacaa acattcaata
    18361 gccaaacata taagatataa ctaacgatta aattattact tatccaataa aaaatgactg
    18421 ccggaaaaaa ggtcaagtag ggataaaaat aattcactcc gctcccataa tgattaaacg
    18481 tcgtaaaatt gattggacca gaaaaaattg aagataaccc tttgactcta gcaaaatgga
    18541 aggcaatatc ccccgatgta gaaatagtat gaacatttct aaaataattg attgaataaa
    18601 gtccagcaat aattgctagg agaatggaca atattatttt tttctttact tgcattactt
    18661 aattatatac tcctttcatg gattgcttta tctagtataa cttaaatttt aaataaatcg
    18721 ttatttattt ttactctcac ttaaaaaagc actaaaatta taagagaggg aggtaatggt
    18781 ctgaacacca agaattgttc caacatcgaa ttgcttaact aatgagttca aagcttaatt
    18841 atgtggctct tatatttacc gagcgtggat tgcgatctct ttagcgaaac aaacatcttt
    18901 cataaaaaaa acagccatat ccgctttctt ggatatgact atttactact actttactat
    18961 ctttcattct tcttgtctca cttatgataa ggcgctgcgt atcatgagtg aatgatatta
    19021 ggaaaacttc ataaaataaa tcttttatac atttccactt ctagatggtc attcatgtta
    19081 gaagaatcaa actatttttt tactgtcgta ataatattaa aacactagaa caaatcaatt
    19141 aaattccata aaatataaat aatagaccta gcatcatctg gtataaaatg gatgctaggt
    19201 ccgttatatt tactcaaaat tgattctacg aatagatctt cttatcattt ctattttagt
    19261 agtcatgcgt tgttgttctt ctaaaggtgg taatggtata agtagtttag ataaatttgt
    19321 accatttaca tttggttgcc cagttccgta agaaattttt tctaataact tccaatataa
    19381 tggcgagtct aagaagcagt ctacgtattc tgataataat ttctcaacta acctaattcg
    19441 aatcaagtaa gaagcaaaaa cagattcctc acttatttcc ttaaccaaga aactttttcc
    19501 catagttcct cctgtccttg ctatcaaaat gtcattttct tccaatctta aatcaactag
    19561 tttagaatta ctaatatcac agtaaggcac actggaccaa tttacacgtc cctcttgtat
    19621 atctgtgatt ctcaaaaact taacattgcc ttgttttttc gccgaatctg tatagccata
    19681 ttgaatctta gtagaaatac ttcctaaata aacccatagt cgaacatcgc tagatgattt
    19741 ttcttgtcgt tctttatgaa caacagtgat catcttgagt tgtccgacta ttttttagtt
    19801 gcagcaatct ttttgggacg cttatttgca tgaagcaacg ttgctaagtc tagtaataaa
    19861 acattggcta acacaggatt ggctttctcg cgatacgctg cggcgccact ttcaaacaat
    19921 actgctagac gatcttctac aacaacgatt tgttccaaat acggtggaaa tttgattcgt
    19981 tttaggatcc ggctaggaga gtcccgctcg gacactaaga gttccgaggt ataaggacca
    20041 taagaacgac tggctaagag ataatggcca taaaaagcca caccttgaat cttatcagat
    20101 gtttttctgc tcattgtcac ggttggttgg attttgtctt tgcggcgaat tgctgttttc
    20161 atttcagcta tcgtcggcaa tagatagaaa gtcaactgtc cttcaccttt tagtgtgaag
    20221 ttgccagata ccagttgatc attgttttga gtcaaatacg atgcttgtgg aatctctgag
    20281 agatcagttg tcagccaata agcgattggc tgtcccgtct cttcagatgt atctgctaag
    20341 atatcgatca aacgtaaagc ggctactcgt ccagtagcag atcctgtgat tgtcaaccat
    20401 aaaagttccc gttttgtatc atatactaag ccccctacat gtggccgttt aggtaaaatc
    20461 agcagtttga tcggctgtcc tgtttctcta tccagtacat gaataataga atgatgctga
    20521 tggtcatagc agtaggcaga aataaacaca aaatcttgag aaaaagccaa cccttgcggc
    20581 gtcatccatt gacaaacctc attttcgccg gtttccggca ccaccattct tgtttctgtt
    20641 aatccaggaa tgttatattg ttttgcttca gccttttccc aagctgcttg aacaatagga
    20701 taccgtttta actttttttc aatatcctga tgtatccgtt gctgcttcca atgcttaaac
    20761 tttaggacat agtaaacaag aaggccaatg accagcaaca ctaaaatcat tattgctacc
    20821 cgctgtgtca tttttttgcc tccttgttct attcgatcaa tcgtccatat accgctatta
    20881 attaccgtaa ctacatgttt cttttactag cgtttcttta taggatcttt cataatctca
    20941 tttatagtaa cattaagaat cccttattct actatagtca cttccacatc aatattgcct
    21001 cttgttgctt tagagtatgg gcaaaatgcg tgggcttctt ctgcgatttt ctctgcttgg
    21061 tccttctcaa gtcctggcaa agaaacttct aaaacgaccc cgatttggta gcccccatct
    21121 tctaattgaa ataaagatac ccgtgctttg acttcagaat cagactcaag attattttct
    21181 tccagcatgt gttgaaccgc gccgttgaag caagatgcgt atccagccgc aaaaagttgt
    21241 tccggatttg tcccttcact atggattcct gggcggtcga tcttcatgtg catacttccg
    21301 tttggtgcct caacttcgcc atcacggcct ccacgattga tcattgctgt ttcgtacatt
    21361 tttttcattg agatcatcct ttctttcgtc gttctatatt aagtgtaacg agtatttttt
    21421 aatctgacaa aggtaatgct tgcattcggc gacatcagtt agacaacatc gatgatcagt
    21481 gatagaagca gtttatctat acaaaaaaag aagggttcta tgatatttgg tgtccttact
    21541 cgccttgagt aacaacacca aatatcatag aaccacttaa tcagcttcac actgcttttt
    21601 ctactttcgt agttgaaaaa aacttaaaca aatcatcggc aaatagttaa agaaaaaact
    21661 taatcgttga aagaagccca attgcttaaa gaaggcaaac tgactaatcg acggaatccg
    21721 tgctaatccg taaagacctg caattagtaa actaataact gttaaaacaa gataaaaatg
    21781 gctaagcgta ccttgaccac tttgacgata aagcagaaca agtaacaatg ggaaaagtag
    21841 aaagcctgca tagcctagcc cagaacctat attatggatc caagtcgata gattacaaga
    21901 gctttcattc gtattgatac tgaataagcc agtaaaaata caatcaccta tcccatataa
    21961 accaactgtt gcaacaacaa gtattgcaag tgtttttgat gttccaacaa ataggtgata
    22021 caacgcaggc aacgaaagaa caaaaaaaag tccagataca actgaccaga ccaagaaagc
    22081 acgtctgact ggactatcta cgtcaccaaa cacactaatt acggtagtca tttgattcag
    22141 tttgggataa aaaaggccaa ggatatacgg tgtcgataaa tcactcatca cacctaataa
    22201 taaaaaataa aaaccatatt tttgcaaaaa aatcattttc ttcccctact ttttagtgat
    22261 tattcgatac atccaacaat caaatttttt ctcattttaa cacatatttt tattctagtt
    22321 gtcttttgaa caatcaataa cataaaggtg agtttcttct acattttaaa aaatcgaatg
    22381 gcactgaaga gttcagttgt cagcatcaat ctcctactca aaaaggcaaa aaaacgtaag
    22441 tcagatgttg ttgacatctc acttacgcat ccattgattg aaaatttctt agtaaaatct
    22501 actagcctaa aaatttgaag acttcttctc gatcatacag tttaaatact ttctcgcata
    22561 aaaattgagt aattagtgcc gcaaaaacag ggatgactaa ccaacaaaga acaacaagaa
    22621 agccatttag ccctgcatct aaagaagcca acggaccaac aagtccgacc acgccgaaac
    22681 cagcagaagc aggcgtacct gaaatagaaa atagagcgac aggaatagct gtaatcactg
    22741 cactaactaa acaaggaata agaatcacag gttttctaaa tagattaggc atcatcatct
    22801 tcatagcgcc taaagcaatc gcaattgtca cacctgactt attaaccttc caagagttca
    22861 ctactaaaac aaaagtggtt gcagctacgc ccatcgcagc cgcaccagca gaaacaccat
    22921 ctagttggat tgccattccg attgctactg ttgaaatagg agagataatc aaaaaggcaa
    22981 aggaacaacc aatcaagata gacattaaca gcggttgtaa ggtagtgaag ttattaatca
    23041 atgatccaat tgcagaagta attgctgtta caaaagggaa taaataaaaa ccaagtaaac
    23101 ctgcacctac acctacaaca atcggtgtta acacaatcga cactgaccca aaactatttc
    23161 caatcagtaa aatcagcccg acagcaatcg atgctgtcag catcgtatta ataatatcgc
    23221 ctgtaccagc accaacgtaa acacccgttt cacccaaatc aggtgtgaat ttgactacac
    23281 cagaaccaac aaaagaagct ccggctacaa ccatcattct catcggatca aatccaaatt
    23341 gtaaggcaat caaaccacca atgatcaaag gtgttgctag ttgaaaaata actgctaact
    23401 gtgcgatcgt taccgcaaag ggatacgcac taaaatattt taaaatggca cctaatactg
    23461 cattaggaat caatccaata atgattcctg ttgctgtacc agcgaggact ttattcagaa
    23521 aaattttcgg tgatattttt tgtgaagttt ccattcaatc tctcctttat tttacgttca
    23581 aactatcttc ttttgcatgg acaagctcag tcaagaaatc acaataaggc gtcgcaacac
    23641 catatttctt cccttttcta gaaattgcgc cattgatata atcgatttct gttaatcgat
    23701 ggttcttaat caagtcttga tacattgaag gataatgtaa cccaattgtt tccgggtcaa
    23761 aacaagattc acaatgtgca atgacttctg ggacatctag ttcaatcttc tctactgctg
    23821 ctactttggc aaactcattg acaatcgtcg ccaccatttt atgagcagtc gatgtttttc
    23881 ctagctcagc catattaacg tctaagattg tgcatagtcc gttcattgtt ccgttcacac
    23941 atgctttgcg ataaatagaa tagtgaatat tgtcagaaaa atgagcattt aatcctgatt
    24001 cagacaattt gtctgctaat ttttttgcag ctgcttcttt tccatcacct aaattttgta
    24061 attctactga accacttcca aataatttga cctgaccagg accttcaaga cctgccgtcc
    24121 acatggtatt tccaatatag atattttcca ttggtacaaa tttctcaata atatcttcat
    24181 gcccgatacc atttaataga cataagacct ctgtatcttt tttgattaat gattggatat
    24241 cctgcagcat tttttccagc tgcatcgctt tggtaaatag aataatcaga tcaacttgat
    24301 cttctttttc tacttcggat tgaagaacga ttggtagttt tgcttctacc tctttaccat
    24361 taaaatttgc ttgtaatcca tgttctttga tttgttggac atgttctgcc cagccatcga
    24421 ttaataatac ttcgttccca ctttggtgaa gcatcaaccc aaagcgactt cccattgcgc
    24481 cagcgcctgc aattgctatt ttcattgatt cttcctcctt tttctttcta ttatactcat
    24541 ttgagaatct tttcacaacc tcgttttgtt attcttctct tatttatcat gtttatatcc
    24601 accattcaag ttattttatc agcaaatcaa taaaaactgg aatagaatcc caatttcatc
    24661 gtaagagaaa ctagactagc aataagaaaa tagaaacatt catagaaaaa atctcactcc
    24721 tctgattata aaagaggggg cattaacctc gtgtatacta aaaccttcca ttcattcgat
    24781 ggatgatttt ctttttgtga aaaaatattc attgtagtac gatgttgcgc aacacagacg
    24841 gatggtattg cttattattt attaaaatcg aacaatacac ttatttaaaa acttctttcc
    24901 cagattttct tccattttta cagttataac tagtatta
//
LOCUS       DARBER010000025        24591 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARBER010000025 DARBER010000000
VERSION     DARBER010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.025"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1778..2443)
                     /locus_tag="SCK06_001054"
     CDS             complement(1778..2443)
                     /locus_tag="SCK06_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1151072.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2623..4605
                     /locus_tag="SCK06_001055"
     CDS             2623..4605
                     /locus_tag="SCK06_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG1151073.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="SCK06_001056"
     CDS             4609..5247
                     /locus_tag="SCK06_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151074.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="SCK06_001057"
     CDS             5275..5703
                     /locus_tag="SCK06_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151075.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6160..7071
                     /locus_tag="SCK06_001058"
     CDS             6160..7071
                     /locus_tag="SCK06_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1151076.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7288..7725
                     /locus_tag="SCK06_001059"
     CDS             7288..7725
                     /locus_tag="SCK06_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151077.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7820..8704
                     /locus_tag="SCK06_001060"
     CDS             7820..8704
                     /locus_tag="SCK06_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1151078.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8928..12095
                     /locus_tag="SCK06_001061"
     CDS             8928..12095
                     /locus_tag="SCK06_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151079.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            12115..12660
                     /locus_tag="SCK06_001062"
     CDS             12115..12660
                     /locus_tag="SCK06_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEG1151080.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12688..13143
                     /locus_tag="SCK06_001063"
     CDS             12688..13143
                     /locus_tag="SCK06_001063"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HEG1151081.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            13140..13898
                     /locus_tag="SCK06_001064"
     CDS             13140..13898
                     /locus_tag="SCK06_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1151082.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13984..14724)
                     /locus_tag="SCK06_001065"
     CDS             complement(13984..14724)
                     /locus_tag="SCK06_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1151083.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14785..16038
                     /locus_tag="SCK06_001066"
     CDS             14785..16038
                     /locus_tag="SCK06_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1151084.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            16360..16587
                     /locus_tag="SCK06_001067"
     CDS             16360..16587
                     /locus_tag="SCK06_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1151085.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16710..17036
                     /locus_tag="SCK06_001068"
     CDS             16710..17036
                     /locus_tag="SCK06_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1151086.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            17104..17817
                     /locus_tag="SCK06_001069"
     CDS             17104..17817
                     /locus_tag="SCK06_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG1151087.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            18127..18978
                     /locus_tag="SCK06_001070"
     CDS             18127..18978
                     /locus_tag="SCK06_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1151088.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            19099..20964
                     /locus_tag="SCK06_001071"
     CDS             19099..20964
                     /locus_tag="SCK06_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1151089.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            21012..22421
                     /locus_tag="SCK06_001072"
     CDS             21012..22421
                     /locus_tag="SCK06_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG1151090.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22529..23230
                     /locus_tag="SCK06_001073"
     CDS             22529..23230
                     /locus_tag="SCK06_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1151091.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            23389..23868
                     /locus_tag="SCK06_001074"
                     /pseudo
     CDS             23389..23868
                     /locus_tag="SCK06_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         8284 a         3995 c         5046 g         7266 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
     5761 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
     5821 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
     5881 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
     5941 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
     6001 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
     6061 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
     6121 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
     6181 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
     6241 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
     6301 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
     6361 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
     6421 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
     6481 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
     6541 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
     6601 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
     6661 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
     6721 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     6781 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     6841 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
     6901 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     6961 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     7021 aatgttttga gtacattcac ttcagattct agtacagcaa gtagtaaata agcggtataa
     7081 ataaaaaata tgaactattt aaatcaataa aactgttcat ctaaatcttt ttattagaac
     7141 taaatcaaca gtagtttaat tactacttga tttagttctt tttattttaa aaattttttt
     7201 tacttagaat ccagcgaccc tagatatttt tctgtataat cttaaataaa gaaaacaata
     7261 tcaaaaaatg cacgtagaag tagataaatg gagtggtttt gcaaatttct tttatttcta
     7321 gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg aagtacaatg
     7381 gatcatttgt atataaaata tattcatcaa ttttccgttt tggtgttagg cacactaatt
     7441 atagcgggga ttgtcgcatt tattattcaa ggccgtgaca tattaacagt acatcaagag
     7501 tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa caaaaagtgg
     7561 aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc taagattttt
     7621 actttattag gtatacaatg cctatcattt tcttttattt cctatatcat tgttttaatt
     7681 ttcctcagtt tcatagtatg gatgttttat cttgtcaaaa aataaatgaa tgtttcatga
     7741 acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta aagaaatcaa
     7801 tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca gttcaggcgg
     7861 tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg tgtttgatga
     7921 agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc gcgaagcggc
     7981 tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca acgaaggaat
     8041 cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat tgggtacggt
     8101 gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga tcaatcattt
     8161 ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact attttatgaa
     8221 tgtcgtcgca attggctcta tccctgaatc aatcaatgat gtagatgcgg agaagaagac
     8281 gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt ccacacaatc
     8341 ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca gtaccttgct
     8401 gataggacta actaactcag tcggcggttt tgaaacatta cttccaaatg caaaagtgga
     8461 tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata cgcttaaaac
     8521 attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct accttacttg
     8581 tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg ttgatggaga
     8641 tgaaggggat aaattacctg tgacaatcaa agtattgccc tcacatttac aggtttattg
     8701 ttaaaatgga ggttgtgacc gaggagttgg cgggagaaca ccgcttgttc gggataaggg
     8761 actgtgacaa gacttttgtc acagcctttt ttttgtacaa aaaagaaatt tacacctaat
     8821 agaaaaattt ttccataaaa atacatattt taaatatgaa agtacaattg gaaatagatt
     8881 aaaacgataa aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatgtg
     8941 taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt
     9001 ttgccgatgt agagaaaaca aacgaatcaa cgcaatcaga gaatcaaagt tttgacaaga
     9061 ctactttttc agaagaatcg attacttctg aaactacaga atctcaaagt gaggcttcta
     9121 ctaataaaag gcaagaagaa aataagacaa gtgagacctc agaagtagct gaacaaacgt
     9181 ctgatagcag tgaagcggca ctggcaagag ctgtttcagc ggatgggtac agcgaagctg
     9241 ccacaacaga agaactggcg cagcttctcg cagacgaatc agtaactaag atccgactga
     9301 ttcagccttt gactttagac agggaattag agatcaaacg agatatcgtt attgattttg
     9361 gtggattcgc tcacaatttc ggcacacacc atatttatat caatgagata cctcagtcga
     9421 tcgagttcca gaattttaaa ggaacagctg cacatccagg tggattgaca ccaaatatcg
     9481 atggaaatgc cattatcatt gcttatatga ccgatttttg gggaagcaga tatcatttta
     9541 cgggagaaat aaaatttacc gggacacttg atttgtatga tgggtccaaa ttaggactga
     9601 tttacgctcc tcgtgcaact gtcacattag atggtgtttc cggtgtgttg gatgtccaac
     9661 cagtgaagga aggactgaat gccgctccgg gtaaagccta tttttgtaga agctatcaat
     9721 tgaatgtgat caatggtagt caactttatg gaccgtatct agggaaattc tacggttttc
     9781 tagattcagg agatgagact ggtagtggaa aaacagcgcc aggaatccat atcttaagcg
     9841 gttcaaaggt ctctcttgat tatgatcggg cagatggcgt tagcgatgaa ggagaggcag
     9901 tcgatacgct acctggtaat gtcatgtttg aagtcagtgg ttcgggaagc gaattttcgg
     9961 taaatacaaa gatcaatatt accaatgaca acaatcgcgg aatcatccaa atgagaggtt
    10021 caggatcgaa agtcactgta tcgaatggtg ggaaaatcac catcaataca gcaactacgg
    10081 gcggatttcg cttgcaagga gattctagtc aaatccatgt atcaagtggt ggacaaatct
    10141 cggtcaaaat ggctggagat gacgatcaac ccgggaacaa tgggatgcgt tttgtaggaa
    10201 aaggcctttc tttgatcgta gaaggagccg gatcagttat cgacatcgat aagcagagtg
    10261 ggcgttccag cgcagttcgt tttgagaatg gtacgcaaaa gctgacagta accaatggag
    10321 gctcattgag ggtaagaaat acaggtgatg gcaaatctta cgtgaacaga ctcaatcaag
    10381 gaaatcaagc gatccaattt tatgacgctt ctggttttct agaaagtcca ggttcagctg
    10441 attttacagt cactggagaa aaatcaaata tcgatatacg cgcagattcc ggagcaactg
    10501 tagatacaac tggagatatt gatttaaatt ttacggcagg tgaaaaaact tatttagtca
    10561 tggtcgggaa aacaggggaa gataagaccg gcatcatcag tggggatgtg ttgaatgtgg
    10621 acttagaatc tcctacctat tttgatttcc aaaacaaacg gattggaagc gagaccaatg
    10681 gcataggcgg ctgggtattc caaggaaatg gagattcttc tttaaagatc gcccattcag
    10741 aaatcgcttt atggcgaaaa acaggcgcaa cattcgataa ctttgaaaaa gacccggatt
    10801 atttttctga tatgacagac ttatcgatca atgggaccga cttaggcaat ttcgtatcaa
    10861 gccctagtgc aggtttaaca aatgaatttg ctcagacagg tgccggaatg aaaaattata
    10921 atcgaatcag tgccaataat cagctcccga tgattgaatc cttaagagtg ccaacgaatg
    10981 cagataaaaa aatctatggg catgtcgttg ttcccgaagg tgtcgaggca aagccaagag
    11041 atgcttggac aggcgaagta gaagtgactt tacgtattac ttacgcagat acgacgaaat
    11101 catctgtaga aattactgct ctcacgcaag gacaaacaga tgaaaatgaa ggggcaggat
    11161 ataacccatg gggagaaggc gaacaaggcg gcttgttcga agttcaagta cccaatgatg
    11221 agagattaaa aacggaggat caagtgcaag ttgttgctgc taaaaaagta agaggtggcc
    11281 aagaagtgac agaactggac tctgccaaaa cgacagtcga tgtgattcct cctgaacctg
    11341 ctaatttaag taccacattg atcaatgcag cttctcgcac gatcacaggt acaggtgaag
    11401 caggagcctt ggtttcattg aaaaaaaatc aagctgtttt gtcagaaaca actattgatg
    11461 atacagggga atttgttctt tctatcccag aaaatcaact ttttgcagga gatgtattag
    11521 aaattgtatt aaacgaccaa gcaggagaag ctactgctaa gggtgtgcag aacaagccaa
    11581 gtaccaacga tgaaattggg aatcacaatc caagtgatac gccagttgtt tatcatgatg
    11641 ccccgccttt tgcacctgct acgaagatcg tagtagaagg tggtctctca tttcttgccc
    11701 cttccaaact cgattttggt cagatcaaag caactggttt gacacaagaa gcatttggcc
    11761 aaatggatat acaggaaaaa ttatggattt atgatcagcg agaaacgaaa aatccgtgga
    11821 tcttaacgat aaaattatct cagcctttca caaatgatgc aggattttct atgcaggatg
    11881 tcctatattt caagaaagga gaggagtatc aattagtgaa tgaagaagaa ctggaaattg
    11941 tcagaggaca aaattcaacg aatgtgcaag cagattattc agaatattta aataacggtc
    12001 gggcgtttaa acttgtctta accaaagaaa atcagatagc tggtaattac tcagcagaga
    12061 taacatggac attagcagat gcgccgacag aataaagatc aaggaggatg attgatgaat
    12121 cgaatatgga ataaaagtca tcgaaacaac tgctctttac ttatgatcct tctccttgga
    12181 ttcatccttt ggcagacacc tgtccatgcc tccgaattca attttgcggt agattttgaa
    12241 ttaccggcta atcagattga taagaacaaa acatatataa atgtaaggat ggagcctggg
    12301 aaaacacagg aaatccccta cacggtcttt aaccgaacag ataaatcagt aacagttgag
    12361 acattgatcc actccgctac gacgaattca aacggtgtga tcgaatatgg agaatcaaaa
    12421 agtaaatttg atgaaacatt aagttatgat atgaaaaact tagcaaaaac agtgggagaa
    12481 ataactgttc ctcctcaaag tgcggtcact gagacacttt cactgaaagc gccagaaaaa
    12541 gagctggctg gtgtggtcgc aggagggatc acgtttcaag aaaaaaaaga gaacgtttct
    12601 aacaaaagta cagttggtgt cgtcaatcag ttttctcaag tgaaagcaat cttgatttga
    12661 aatagcgaaa aagagtcttc cccagatttg aaattaaaaa aagcttatgc ggatcaaagc
    12721 gatggaagaa atgtgattaa ggcgaatctt caaaatccaa atgcttctta tatgttcggc
    12781 ttgactacat cggttgaaat ctacaaaaaa gaaacaaaag agccttacct ttcaataaaa
    12841 aaagaaaaaa tagatgtagc acccaataca aattttgatt tacttgtccc attagaagga
    12901 aaaaaattag ctccgggaca ttatcaagcg gttgtgaagg gtgcttggaa agaaaaacgc
    12961 tggaatctcc ataccgattt tgaaataact gatgatgcag cggaccaatt gaatgaaaag
    13021 gatattgatt taaaaaatca gccagaaaca tttgactggc gatggctgat acttgttgga
    13081 gtcattctta ttggcttatt tgctggatta tttattttaa aagaaagaag gaaaaagaaa
    13141 tgaaaaaaac aattgcatta agtaccgttc tagctggaag tctattatta tgtacacaaa
    13201 gtgtttttgc tgagaataag agctcagcca ctacagaagg aacgatttct gtgaccaaac
    13261 cagatgctgc aaatcctgaa gacgttgttc cagttcctcc tgttgatcca aatgatcctg
    13321 atactccttt tacacctgac gaaccaaatc agggaacatc cggattactg acgattgacc
    13381 aagctccaga tcttgatttt ggaacaatca agttaggtac agcaaaaaca gtatatgctg
    13441 cattacaaag cggaacggac agtactggag cacaaacaca agtacagaat ttcgtacaag
    13501 taacggataa aagcggaaat tatgccggtt ggaaattatc agtcaaacga acagaattta
    13561 ttggtacaac atctgatacg aataaactaa ctggttcaaa ggtgaaattc aaaaacgccg
    13621 tgattcgaaa tagcgcaact aatagcagtg gaatgccatc taccgtttct ggaactggcg
    13681 ctgaaggaat cgaaatccca gcagatcagg cagtagatct agtaactgca ggagcaaatg
    13741 aagggattgg cacatggatt tattcattag gagagaatgt ggaacaaggg agacaatcag
    13801 tggaattatt tgttcctgta tccaattatg ctgtagacaa ctacgtttca acattcacat
    13861 ggtcgttaac agatgctcct acagaagagg aagcgtaaaa tcatatcaac gatttcaggg
    13921 agtggagccg attcaaggcc cactccctga aatctgctat gcttacgtct cagctcttat
    13981 tcatcaactc gaagcagttt tttggaagtc atggaaacgt tcctttgtct tttcaaagat
    14041 ctttcgacca acgagtaagg agtcaccgtt gaaaaattct aacacataat gagcagaatc
    14101 aattttctgc aattgcgata agttgataca gtaagatttt aaaagtaaaa agtaagcata
    14161 cttttctaac tctgtcttca attgttttaa agaagtattc actaatttat cttctgtagt
    14221 tgttttgacc ataacttggt ttttcaaacc tttgatactt tcaatatata aaatttcccg
    14281 aggatacagg acgagacttc cacctttttg atcaataaag attttatcga catttacgat
    14341 ctgctgttct ttccaattga tctgttctag cacttgacga atttgttcag ataaattcct
    14401 gtcttttctg atatatccaa tcggaaggat tttccgattg atgatctcta ctgcaaaatt
    14461 cgtataagcg gtgaaaaaaa caatattact atgtgcattt tttttacgta tttgttcggc
    14521 gacatcaata cctgtgagtg attgcttcaa gtcgatatcg ataaagaaaa catcgtgatt
    14581 atcaatagtg cccaactgga tcaactgatt gatttgagag atagaagtga gctgcacgat
    14641 attctcgata tgatgattga ccaattctct gatgatccat tcgctgtgaa actggttatc
    14701 ttctatgata aaatactttt tcatatgcag gctcctatta aaaaaatatg tgtacatttt
    14761 gaaacaaaac aatggaggaa taagatggtc gcaacagtga cactttattt gacctttact
    14821 ttgattttag atttaggtat ctatttattc ttttcgaaac aactgctagt aaaaaacagc
    14881 tttggttgta tagctttgat ggtggtgaca cttttacttc ctatccctaa agtgagcttt
    14941 ccttacctag ttggcttatt attgatgttt cttttttatt ttactacgaa aaacctgttt
    15001 atcagctgtt tgatcagtaa tctttttttt gctatcactg gactcagctt ttttctggtt
    15061 tttgatctga tggattattt aggaaagaga catcttggaa cagcaagttt ttttgcacta
    15121 cttatcgcat ttattttcta tctattagta aaaagtatag acaaacagac gagatttctc
    15181 aacttgcttt ttacttatcc aactgaacac tattttggag gtatttgtat tttattaagc
    15241 tggggcgtga tttacttggt attgggaagt gttcacttcc gctcatctaa ttatctgctt
    15301 ctatctcttt tgtcgatagt catcagtttt ttttctttgt ttttttccat tatgttgatc
    15361 agtttccaag taaaagaaag acaacatcag gaatcgcttc aactgtatca aataaatgaa
    15421 gaatattacc atcatctaga ggaattcaaa catgattata aaagtctact gttttcatta
    15481 aaaattactc ttcaggaaaa taaaaaagaa acgttggaat ttttagaaat attagaagat
    15541 cattctgcag ctatttttga aaaaccgcaa atacaacagc taacaaatat tgtatcccca
    15601 gctgtcaggg gtgtcttttt aaaatttcta gaaaaagcag aatcggagaa aatcccgatc
    15661 caagtagtta ttcctgtcga agtgaaaaca atcccgattg atctggttgt ttttattcgc
    15721 tgtttatcta tttttatttc taacgcattc gatcatcgtg tagccgatca gagtccgatt
    15781 acgatcaatc tgctggaaga aaaagaggga tcacgtttta gtatagcgaa caaggcaaat
    15841 ccaacgaatt tgacgctatc ggagatggtc aaaagaggaa aaacctctaa aaaaaatggc
    15901 gggttaggtc tatacagtgc gaaaaaaatg ttggatgttt acgaaaatgc agaactaaaa
    15961 atcgaatttt caaaacagaa tcactatttt tttgtagaaa tgtatctggc atctagaaag
    16021 agcaaatcag cctactgact tactgtcaaa ggataagttt gaggaaagtc tacacggaga
    16081 tagagacaac aacacagcat attcaaaaga aacagaagcg tgatgaccgt tgtcaccctt
    16141 ctgtttcttt tgctatgtag ttaaacaaag tagttgacac ttccgctact tagtattagt
    16201 acgaaagtat acttcatgat actaagtaga taagaagtga aaagcggaac ccacgatcgt
    16261 caaaatgcta actgctttgc ttacaccaga taaagggaag agcaatatcg cttattttag
    16321 gcgtggagaa gcaagcaaat ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga
    16381 gagttagaac aagatgaaag tatccatcga aaagcctata accaagtccc gcctaaagta
    16441 gagtactcgc tcaccgaaga aggaaaatca ttaagagaga ttctagtagc gttgtctgtt
    16501 tgggaagaag aacgtatcga aaaacaaaaa gcctcaaggg aagaagacac gctactgcat
    16561 acacatggtg gctacctaaa ttattgattt tctatgacat ttattttaaa taaaagaaaa
    16621 caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa
    16681 gtaagaagtg attggaagaa ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta
    16741 gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatc tctatttctt
    16801 tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa
    16861 cacaattaga acgaatcaaa gaagaactgg cagatgtatt gatctattca tacatgatgg
    16921 cagacaacct tcacctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt
    16981 tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat
    17041 gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag
    17101 aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg
    17161 ttattagtag gactagcttt gattttcaat gaacagatca aggattattt tgtcagagag
    17221 acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat
    17281 aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg
    17341 tcgcaaatga gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca
    17401 atcaatttac cggttttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca
    17461 ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg
    17521 aaccctgaat tgctgtttac accgctagaa aatttagaga tgggtgccaa gatctatttg
    17581 acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca
    17641 gatacccaat tactgaatga agtcgaggga aaagaaattg tcacactaat tacttgtggc
    17701 gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacatcgata
    17761 aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat
    17821 actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa
    17881 accaatttgc tgaaaaaaag agaaacgcaa agaggtgcat ctctgatttg gttgttttct
    17941 agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa
    18001 ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca
    18061 gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga
    18121 agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc
    18181 ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaagat tggggacgag
    18241 atttcgccag aagcggtcca aaaagaattt gttttaagag attcttttgc taaaaaccaa
    18301 ttccagcagc tgatggctga tattccggca gaggagatcg aatgggtcaa acaagtagtc
    18361 gaactggctg aagataaact aaaagttgaa ttttcgccta atatttatct gacattgacc
    18421 gatcatcttc attatgcgat cacacgagca aaagaaaata tccaactgcc taatccatta
    18481 ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga ggctcttttg
    18541 ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg aagccggttt tattgccttt
    18601 cattttgtga atagccaaca aggaaacgaa gacatgcaag tgacgatgac cgctaccaca
    18661 atggtaagag atatactgag tatcatcagt aaattcttcg gcatcgtgtt cgatgaagaa
    18721 tctttgaatt atcagcgtat cattactcat ctgcagtttt ttgcccagcg ttatctcaaa
    18781 ggggaaggat ctgacgagca agatgaattt ctctacgcat tagttcaagg aaagtatcca
    18841 aaagcgtttc attgtgtcga acggatcaat gagtatcttc gacaaacgca aaaacaggaa
    18901 atggggatcg cagagcagat ttatttaacg attcatattc aaagagtagt aagtgagaaa
    18961 aaaagcatcc aatcataagg attgttactg tatggcaggc aaaacctgga ttgaaaaaat
    19021 aggttcacga atgtttgtga ctgttttttt caatctgggt ttttattttt taaataaaaa
    19081 agaaagaagg aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg ggaagcggta
    19141 ggaggacaaa agaatgtcaa aagtttagta cattgtgcta cgcggttgcg attcagatta
    19201 aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag acccggacgt gatccaagtc
    19261 gtacaaagtg gcggtcagta tcaagtagtg attggcagta atgtagcaga tgtttatcaa
    19321 tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga gtccaaaaat
    19381 attttgaatc gtctgatcga tattatctct agtatattta caccttttct aggggcgatg
    19441 gcagccgccg gggtattgaa aggattctta tcattggcaa cggtgatggg ctggctgaca
    19501 gctgatagcg gtacttatca aatattgttt gctgcagcag atggtgtctt taccttctta
    19561 cctgtcatgc ttgcttttac agcagctaaa aagttcaaag ccaaccagtt tttagcagta
    19621 gcaattgcta tggcacttgt gtatccagct atcacagcag ttgctggtgc gggagaagct
    19681 atcagtttct ttgggatccc agtgattctt tcaccatctg gttatacttc ttctgtcata
    19741 ccaatcattc tagccgtatg ggttcaaagt aaattagagc cattcgtaaa aaaagtgatt
    19801 ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt tagtagtgat ggttccatta
    19861 acatttcttg ctttaggacc aatcgggacg gttgccggta atgctttagg cggtctcttc
    19921 aactcgattt atggatttag tccaatcgta gcaggtctaa tcatgggaag tttatggcaa
    19981 gtattcgtga tgttcggcat gcattgggga ttcgtaccga tcatgttttt gaatatcgaa
    20041 caatatgggt ttgatgtatt gatgccaatg cttcttccag ccatcttggc acaaggtggg
    20101 gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt agggatttct
    20161 tctacagtga cttcgttatt tggaatcact gaaccaactg tgtatggtgt gacattacct
    20221 ttgaagaaac cattcattgc cgcttgtatt tctggaggta ttggtggagc tattatcgga
    20281 ttttccggag taaaagcttt ctccagcagt ttggtcagct tattgacgat tccgactttc
    20341 attaatacgg tggatggtgt agagtcaaat gtgacagtag cagtaatagc tacaggaatt
    20401 gcttttgttc ttgcattcgt aggtacattg attttaggat ttgacgaaca aacacaagac
    20461 aatcaattag aaaacaaaca tgcgaatgca ggaaaaccga ttacttcagc acggcatacc
    20521 ttaaaaagtc ctttaacggg taaagtactg ccattgagtg aagtaccaga ccaagttttt
    20581 tcttctggcg tgatgggaaa aggtatcgct attgatccag aagtaggaga actagttgca
    20641 cctgcagacg gagagatcac gactattttt ccaactggac atgctgtagg gatcacaacg
    20701 actgacggag cggagatttt gattcatatc gggatggata cagtagaatt aaatgggaat
    20761 ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag caggagattt attgattcgt
    20821 tttgacatcg aagcaattag agcagctggc tatagcgtaa tcactccagt tgtcattacg
    20881 aatactgatg catttgccga tatacttgaa ttagaccaaa aagagattat tgcaaacgaa
    20941 gatgttctgg caattgtaaa ataaggagga aatcaggatg caatcacgtt tttcagaaaa
    21001 atttttatgg ggtggagcaa ctgctgccaa atcaattaga aggggcttat gatcgagacg
    21061 gaaaaggact atccgttgca gatgctatgc ctggaggtaa gcagcgcttt gcgattatcg
    21121 gcagtgaaga atttgactgg acgattgatc aagaaaaata tatttacccg aatcatcgag
    21181 gaatcgatca ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa atgggattca
    21241 aatgttatcg tttttcgatt gcctggacac gaatttttcc aaatggagac gaaggtactc
    21301 ctaacgaagc gggattagaa ttttatgatc agctgattga tgagtgctta aaatatgata
    21361 ttgaacctgt gatcacgatt tctcattacg aaatgcctct tcatttggca aaagaatacg
    21421 gtggctggaa aaatcgaaaa ctgatcgact tctatgaacg atttgctcag acagtattag
    21481 agagatacag cagtaaagtc aaatattgga tgactttcaa tgaaatcaat tcggcctttc
    21541 actttccagc tttaagtcaa ggattagtaa aaagcaatgg agcaggagag taccaaaata
    21601 ttttccaagc atggcataat caattcgttg caagtagcaa agccgtaaaa atcggccacg
    21661 aattgcgttc ggatatccaa atcggctgca tgattatcta tgcaacaact tacagcatcg
    21721 atgccaatcc agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc aattttttct
    21781 gcacggatgt ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc cataaaaaat
    21841 atggagtcga tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg ctaaaagaat
    21901 atccagtgga ctacatcgga tttagttatt atatgtctac ggcaatcaat gagactgatc
    21961 cacaagcagc aacttcagaa gggaacttgc tgggaggagt aaaaaatccg tttcttgagg
    22021 caagtgaatg gggctggcag atcgatccag aagggctgag aatcgcattg aatgaattat
    22081 acaatcgtta ccagaaacca ttattcattg ttgaaaacgg tcttggagcc attgatcata
    22141 tggaagagga tggcacgatt attgatgatt atcggatcga ttatttacgt cgtcatatcg
    22201 aagcgatggc taatgcagta gcagacggtg tagatttgat gggctatacg ccttggggat
    22261 gtatcgatct tgtcagtgca tcaacaggag aaatgagtaa acgttacggc tttatctatg
    22321 tggatctaga cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa tcttttgatt
    22381 ggtataaaga agtaattgcc tcaaatggcg taaacttgta attaaagcag ctgaatgaaa
    22441 aagaacacac ttctcacatg tgatttgtgt tcttttttta ttttttatgt gctattcttt
    22501 tagtaatagt aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat acttggaagt
    22561 cgcagataaa attaaagaag atattttctc aggaaaatat ccagtcggtt cgatgttgcc
    22621 gacagaaagt gaattagaag aactgtttca agtcagcaaa atcactgtcc gaaaggcaat
    22681 cgaactgctg gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga cgacggttct
    22741 aagcaatcgt ccttacaatc gattatccaa agcggcaagc tttacgcaaa tcttggagaa
    22801 ctcccgatat gaagtccgga aagaaacgct ggatttgcaa ttggtccata taaagaagga
    22861 agatcctttg ttttcctttt tcggtgagca agctgtttgt tttaagcgct tatattattt
    22921 gaacaatcag ccttatattt attttgttca ttatcttcca caagaaatga tgcaattcag
    22981 cagagaagaa tttggcaaag aatctttgta tcgtttgctg acgaaaaaag gatatactat
    23041 tgactcattt tctgatgatt tctcggcagt atttttaaca gaagaagaac aaggaattct
    23101 gaaaacagac gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc aaattgggga
    23161 aatgattgaa tattcggagg cggtctacca tacggagctg catccttacc atattgatta
    23221 tgaaacataa ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    23281 gccaaacaaa aatagcttct catatttttc gtttggttag gcttattacc gcagatacac
    23341 ggctgtttct ggattaccat ttattctgga taagggggtg tggcacgatt ttcagaagga
    23401 atctatgaac caatcactgt attgatttta gcttcgatgg taaccccatt cggcttgacg
    23461 atcgcttatt tcttagggaa aatcattcgt aaaaatatac tcaatcgtca agaaatcgat
    23521 acattgaaga ccgcttttcc tatggggatc tgtcagatca cagagggatg tttccctatc
    23581 gtattgaacg acttggcacg caatgtcata gcaacgggag tcggtgggac agtaggtggc
    23641 ggattaagca tgttttgggg cgcagatagc cgtattccgg catcgggaat gtttgcagtc
    23701 gctacgatga ccagaccatg ggcattcatt ggtgcattgt tagctggttc ttttgtcaca
    23761 ggaataacca ttttattatt gaaaaaacca gtagatccga atgcagaaat catccaagaa
    23821 gaaaaagagg aagaagacat atcttgggat gatttgacaa ttagttagga gaggcagaat
    23881 ggattacaag aaaaaagtac aatagatgta ccttgagaca ggaatggtat gaaagaatca
    23941 gagttagaaa gaatcgaata tacagatttt ggtttaggag cgatcgaaac tgtccagagc
    24001 atcggcaccc tccttgaaat gacgatgaag gaaaaatgag aacctttcgt tgtcgaaagg
    24061 gtctggtata ccttatgtcg aaggaaagga aacaaaaaaa ctttcctgta ctattccaaa
    24121 aaggaaagaa agttattata cagtaaaaaa agaaattatt cttcatgcta gagatcagta
    24181 cacgttcgag cccaatatca aacactagtt ccaggcaggc gcaacaggtg cggttatcac
    24241 tgaattttct tccagcagtg acgattcaag tgatattttt acaaatccag ctatttatcg
    24301 ttaacaaaaa gatttttagg agaagctgat gaacaaaaat gatcaaattt tgatcgttta
    24361 gttcgtcggc ttttttattt agagaaacat catttatcag tgaaacataa cacgagaaca
    24421 aaatgatttt ttcgttaata aaagaaacaa gtatttgttg tttaaaataa tacacgttat
    24481 tggcatatag gacgaaatcc cagtatcttc tataagtaag tcatttcata tcgatgaaaa
    24541 cacgttattt atttttgttt tatctataaa atggctgaaa aatgattttt t
//
LOCUS       DARBER010000026        23829 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARBER010000026 DARBER010000000
VERSION     DARBER010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.026"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(53..787)
                     /locus_tag="SCK06_001075"
     CDS             complement(53..787)
                     /locus_tag="SCK06_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1151092.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="SCK06_001076"
     CDS             1114..1524
                     /locus_tag="SCK06_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG1151093.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="SCK06_001077"
     CDS             1517..2218
                     /locus_tag="SCK06_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG1151094.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="SCK06_001078"
     CDS             2218..3804
                     /locus_tag="SCK06_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1151095.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="SCK06_001079"
     CDS             3823..4044
                     /locus_tag="SCK06_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG1151096.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="SCK06_001080"
     CDS             4041..4802
                     /locus_tag="SCK06_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG1151097.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="SCK06_001081"
     CDS             5182..5691
                     /locus_tag="SCK06_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1151098.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="SCK06_001082"
     CDS             complement(5766..6305)
                     /locus_tag="SCK06_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG1151099.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="SCK06_001083"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="SCK06_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG1151100.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="SCK06_001084"
     CDS             7318..8169
                     /locus_tag="SCK06_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG1151101.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="SCK06_001085"
     CDS             8197..8832
                     /locus_tag="SCK06_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG1151102.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="SCK06_001086"
     CDS             8852..9625
                     /locus_tag="SCK06_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1151103.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="SCK06_001087"
     CDS             10033..10830
                     /locus_tag="SCK06_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG1151104.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="SCK06_001088"
     CDS             10808..11221
                     /locus_tag="SCK06_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1151105.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="SCK06_001089"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="SCK06_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1151106.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="SCK06_001090"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="SCK06_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG1151107.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="SCK06_001091"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="SCK06_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG1151108.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="SCK06_001092"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="SCK06_001092"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG1151109.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="SCK06_001093"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="SCK06_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG1151110.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="SCK06_001094"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="SCK06_001094"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG1151111.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="SCK06_001095"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="SCK06_001095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG1151112.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="SCK06_001096"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="SCK06_001096"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG1151113.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="SCK06_001097"
     CDS             21905..22483
                     /locus_tag="SCK06_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1151114.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="SCK06_001098"
     CDS             22535..23017
                     /locus_tag="SCK06_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG1151115.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="SCK06_001099"
     CDS             23134..23508
                     /locus_tag="SCK06_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151116.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6648 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atagaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DARBER010000027        23634 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARBER010000027 DARBER010000000
VERSION     DARBER010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.027"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            82..1422
                     /locus_tag="SCK06_001100"
     CDS             82..1422
                     /locus_tag="SCK06_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1151117.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1640..2083
                     /locus_tag="SCK06_001101"
     CDS             1640..2083
                     /locus_tag="SCK06_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG1151118.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2587..4005)
                     /gene="nhaC"
                     /locus_tag="SCK06_001102"
     CDS             complement(2587..4005)
                     /gene="nhaC"
                     /locus_tag="SCK06_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1151119.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4408..5631)
                     /locus_tag="SCK06_001103"
     CDS             complement(4408..5631)
                     /locus_tag="SCK06_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG1151120.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5662..6501)
                     /locus_tag="SCK06_001104"
     CDS             complement(5662..6501)
                     /locus_tag="SCK06_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1151121.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6518..7198)
                     /locus_tag="SCK06_001105"
     CDS             complement(6518..7198)
                     /locus_tag="SCK06_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151122.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7200..8273)
                     /locus_tag="SCK06_001106"
     CDS             complement(7200..8273)
                     /locus_tag="SCK06_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1151123.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8329..8567)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8610..8843)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8920..9591)
                     /locus_tag="SCK06_001107"
     CDS             complement(8920..9591)
                     /locus_tag="SCK06_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1151124.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9678..12356)
                     /gene="mgtA"
                     /locus_tag="SCK06_001108"
     CDS             complement(9678..12356)
                     /gene="mgtA"
                     /locus_tag="SCK06_001108"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG1151125.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            13015..13452
                     /gene="ndk"
                     /locus_tag="SCK06_001109"
     CDS             13015..13452
                     /gene="ndk"
                     /locus_tag="SCK06_001109"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG1151126.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13474..14340)
                     /locus_tag="SCK06_001110"
     CDS             complement(13474..14340)
                     /locus_tag="SCK06_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1151127.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14500..16593)
                     /locus_tag="SCK06_001111"
     CDS             complement(14500..16593)
                     /locus_tag="SCK06_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1151128.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17342..17965)
                     /locus_tag="SCK06_001112"
     CDS             complement(17342..17965)
                     /locus_tag="SCK06_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151129.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18124..19521)
                     /locus_tag="SCK06_001113"
     CDS             complement(18124..19521)
                     /locus_tag="SCK06_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1151130.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19553..20524)
                     /locus_tag="SCK06_001114"
     CDS             complement(19553..20524)
                     /locus_tag="SCK06_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1151131.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20898..21323
                     /locus_tag="SCK06_001115"
     CDS             20898..21323
                     /locus_tag="SCK06_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151132.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21811..22512)
                     /locus_tag="SCK06_001116"
     CDS             complement(21811..22512)
                     /locus_tag="SCK06_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1151133.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22528..22968)
                     /locus_tag="SCK06_001117"
     CDS             complement(22528..22968)
                     /locus_tag="SCK06_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1151134.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22968..23543)
                     /locus_tag="SCK06_001118"
     CDS             complement(22968..23543)
                     /locus_tag="SCK06_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG1151135.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6796 a         4899 c         4065 g         7874 t
ORIGIN      
        1 gttatcatta gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa
       61 tttaaaagaa agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga
      121 catatcacca tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc
      181 gaagcgattg ggatcgccgg cccttctgta ttgattgctt ttttgattgg tgggctgatt
      241 ttatatatcg tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac
      301 ggattatctg gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg
      361 gtctactggt ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt
      421 ttgcagttgt ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta
      481 actacgatca tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct
      541 tttgctaaaa tcagtgtcat tatcttactg atcatttttg ctacttatct tgtcggtcag
      601 caaatgttgg gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt
      661 actcctcatg gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc
      721 ggttctgaac tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct
      781 aaagcaatca aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg
      841 cttttgattc tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg
      901 gtatttgaaa aaatgaatat cccatttgca gcggatatcg tcaattttgt gattgtcctt
      961 gctttgttct catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt
     1021 ttgaaggatc gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg
     1081 caacgtgctg ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt
     1141 gtcggtgaca aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc
     1201 tggatcatta tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat
     1261 aaaggaatca gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc
     1321 acgaactcca ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt
     1381 tatcaaaaaa agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta
     1441 ttttatttat gtggaataga tggagctatt gaagaaagga gttgtgacaa aagtcttgtt
     1501 gcaactcctt tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc
     1561 ctctgtgcaa aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa
     1621 aaaaaggagt ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc
     1681 aacggtagtt atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt
     1741 cagtcgtaca cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct
     1801 gttgttcatg tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag
     1861 tcttcttgtt tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac
     1921 agcttggatg aaggccatga ttcctcatca ttctatcgct atcctggcaa gtaaacgagc
     1981 tgatttaaaa gatcctgaag taaaaaaatt agcggaagaa atcattcagg cacaagaaga
     2041 agagatcgac aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat
     2101 aaaaaaagag gatgttccag aagttatcag cttcaagaaa taagaagaag ctacttctgg
     2161 aacgccgtga ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     2221 acaaaaatag cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt
     2281 tgaacaccgt ttattaagag gttgtaaaat cagcgttctg acctgagtat tagagagaca
     2341 aatggaaaaa tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga
     2401 ttgaacaccg tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa
     2461 ttttgtgaaa tggctttcca catttttgta taaatttggc ttaataccga gaaactggct
     2521 tttgaatacc gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca atcccttttt
     2581 atgtttttat tgttctaatt gcgctgtctt gcttactgct tcttgcggtt tcacaaattt
     2641 ttttgagatt ccccaacctg tgaaggcact gaccatcgaa aacacaggag aaagcaaact
     2701 gaaaaatacg aatggcaagt aagtgagtgt aggaatacca aatgtacttg ctacgaaact
     2761 tccggcaatt ccccaaggca ctaagtagtt gattactgtt ccaccatctt ctagcgttct
     2821 acctaataca gttggatcaa atcctgcttt ttcataagtt tctttaaatg cattaccagg
     2881 cagaataaca gataggaatt gttcaccaat aaaaatattt actccgatac ttgtcatcaa
     2941 tgttgtaaaa atcaaatttg gtgtgttcgt catctttctt gatactcgtt ccatcacggt
     3001 tgagatcaag ccaaattcga tcaacaatcc acctaatgac aaagttagaa taatcaatga
     3061 cacagtaggc atcatacttt cgattcctcc gcggctaagc agtgaatcga tttgttggtt
     3121 tccagtattt gaaacaaacc ctgattcgat gaccccagcc aacttcgtta aggaagtttg
     3181 tgggttttga ataaaaatca ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat
     3241 tgccggcacc tttttccatg cacacagtaa cattaaaaat aaaggaatca gtgaccagat
     3301 tgaaatagta aagtttgctt ctagaacatc agtcacttca cgaatagatg aaagacttgc
     3361 ggctttactt gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac
     3421 agttgaccac atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc
     3481 tagatttgtt gattcggata atggagacat cttatctcca aaaacggcac ctgaaacaat
     3541 agctccaaca accagcgctg gatttaagcc tagtgttgtc ccaataccga aaaatgcaat
     3601 cccaatcgtt gacataacag taaaggcact ccctacagcg ctacctacga ttgcacacac
     3661 aacaaataca gaagggacaa accactggac actgatcaat ttgaacccaa tgaccattag
     3721 agttggaatg atccctgctt gtatccatac agcaatcaat gaaccgacta aaataaaaat
     3781 aaagattggg atgattcctg gtttgatgcc attgatgatt ccttcgtgca gccaatctgt
     3841 cgggtatctt tttatcactg catacaacat gaccattccg atcgctgcta agatcgtgat
     3901 attaggtgag acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa
     3961 tacaagtgtt gcttctaaaa aacttggtgt tccttttttt cccatgataa tctctccttt
     4021 tttagttgtt cttatgttct aaaaaagtta agatatcgga ttactccgcc gcaattcatc
     4081 cgcgattcct cctttataaa aataaaaaga cctgttttaa actaaggacg aatgactccg
     4141 cggtaccacc ttaattaaaa acagatctgt ttttcacttt caaactttta ttcaaatgta
     4201 tggactttcc atgtaattcg tcaattaggc ttgatcggtt cacagcgacc accgactttc
     4261 tgaacaaaac acctaaccgc tactgcgtga aaagttgact tgtacgaagt ttaccttcca
     4321 taagatcatt tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa ttatttttat
     4381 aactttcaca cacaagttta ttttttacta gaaagtagcg ccctcttgtt tttttcttgc
     4441 tactatttct tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc
     4501 aagagctgta ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact
     4561 tttagcagca gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac
     4621 cgtaggctgg attgttggta tgacttggct gacatttcct acatcagaag aaccgagact
     4681 tgttctttgt tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa
     4741 gaataagtca tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc
     4801 aaactcaaaa gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc
     4861 atatacttta tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta
     4921 atctggaatg acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc
     4981 ttttggcaat tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa
     5041 agcatttact cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat
     5101 gtcgactgga tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac
     5161 gcataacgct acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga
     5221 tccgttctcg cccccttctt cccctggggt gccatataca cggatttctc caccagtttc
     5281 atcaatgaat tgcttcacta tgctggcggc taatacggag taagtaccaa acaaattatg
     5341 cccgcatgca tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg
     5401 accgggttta ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt
     5461 ttctacccaa aacctttctt tttctaattg tttaatcaac gctgctgagg aataaaactc
     5521 atagttactt acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa
     5581 acgatattgg attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc
     5641 ctcctatgct agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga
     5701 tccttttgat gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat
     5761 tttttgatac gttttattgt tttcatcttc ttttcttgca acgatgatat tcacataagg
     5821 acgtgcatgg tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc
     5881 atctacagct acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt
     5941 ttgggaagca tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac
     6001 agtgggcgct tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag
     6061 aagagctctg ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat
     6121 atcagctaca tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc
     6181 gatcgatact aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg
     6241 gaaagaattc aaatcaatcg atccatctgc taaagccaca tttggttgat tgtaatctgt
     6301 aaatttcacg tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca
     6361 tacatctgta tcttctccaa taatccctag ttttacgata gtttcttttt tgtcagttgt
     6421 atctgctttt ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac
     6481 aaaacgaata atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca
     6541 gttgattact gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa
     6601 tcgttacatc tgtttggaat cgattatacc ctctcgatat agcaagattc cctaatccgc
     6661 ctgcacctac tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg
     6721 agacccgaat gataccaggc aaaccttcta acaaatacac acgtaaaatg ataccgattg
     6781 gactggttcc catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt
     6841 tctcgatttg acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt
     6901 tcgcaccaat cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga
     6961 taatgaaagg gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac
     7021 ggttagctag taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccaa
     7081 tcatcccgcc aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga
     7141 caaattcatc cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt
     7201 tatcttcctc cttcgatcac tttgatacga ctatctaaat cgtttttttg taaaatattt
     7261 atttttacac cagatttgat caaataatcc aatgcttttt tccgctgttt ggtttctcca
     7321 gctaaagtaa cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat
     7381 aaaatattcg ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc
     7441 tgatttccaa tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca
     7501 gaatgggcaa tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct
     7561 ttggtcaatt cttcttcagg attgctgaag atctggatgc tgctaccttt ttcaacgatt
     7621 cttccatctt ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga
     7681 gtgatcagga cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc
     7741 tgaagggtcg ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt
     7801 tcattcgcca aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta
     7861 ggataagcag atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga
     7921 cgacgttcct gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata
     7981 gttcggcttt ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt
     8041 cgcaattctt tgggagagag ctgactcaga ttcgtctgat tgacaatgac ttctcctgct
     8101 gttggtttct gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa
     8161 ccgacgattc cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt
     8221 atctcttttt ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt
     8281 cctccttatc taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct tgaggacgct
     8341 tttttgcgtg ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac
     8401 gatcatactc tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat
     8461 gaattgctcg atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct
     8521 ctctgtcaaa ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa
     8581 agtcctttta ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt
     8641 tttcggaagt ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt
     8701 aacggtcgaa cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt
     8761 cagcttttct tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt
     8821 actttccttt tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact
     8881 gaaaagtcaa gtacgaaatt caaattattt tttctatatt tatttttctg tttttttatt
     8941 taacatttca ataacagttc gaagatcttt tccttccgca agaagtttca atttttcctt
     9001 ttcttcgacg gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc
     9061 tgctcgtgaa gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc
     9121 ttctaatcct gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga
     9181 cacttcgtct ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt gatccaagat
     9241 atctgttgct cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt
     9301 taagtctgcc aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg
     9361 ttgataaaaa tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc
     9421 tgctttcact aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat
     9481 ttcttttggg atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt
     9541 tttttctgtg tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt
     9601 aatcctttcc aaaacaatac acatagttta gcacgactgg tccaaaaaga acagaagcaa
     9661 agaaccagcc ttctcgttta taagagttct tggaatcgac ggatatagat tttcttcaca
     9721 gctgtcacaa gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca
     9781 ggaagtggca tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac agaaccgaca
     9841 ataattccta atgtagtgat aacaaacatg gcaaatgttg cgttgctttg gataaatggt
     9901 actttcggtg tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac
     9961 catcctgcat ggaataatgc gatgaaaatc actcgctgtg aactatccaa tgtatggaaa
    10021 cttccgccca ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca
    10081 aatatcgaac ttgttggacc aaagtaaacc atgaattccc caatactaga tgcattccat
    10141 ttttttggct cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa
    10201 acatcataga tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg
    10261 aacgtacttg caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg
    10321 atatatttca tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc
    10381 gtcaaatctt tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta
    10441 tctacagaaa taccgatatc agccactttc attgcaggag cgtcattgat accatctcct
    10501 aaaaatccga cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga
    10561 ggattcaatt ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc
    10621 atttccgaga ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac
    10681 actgaacgtg taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct
    10741 ttcagagctt tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agcaagataa
    10801 ccaatcaaaa ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg
    10861 gtcttttgtg cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca
    10921 agtacggctt cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat
    10981 ttagaaatgt tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct
    11041 ggatcagcaa ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact
    11101 ttttgataat tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca
    11161 tctaacaaat ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca
    11221 cgatcatctt catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc
    11281 aatgtccccg ttttatccgt acataacaca tcgattgcac caaagttctg gatggcattc
    11341 aaatttttga tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc
    11401 gtaacgatca ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga
    11461 aaagcttcta gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc
    11521 aatgacataa atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat
    11581 tgaattggtt tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca
    11641 gaaacaatga ttccttccgc acttccactg atgacattac ttcccataaa gacaagattt
    11701 tcgtaacttg tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga
    11761 tagctttccc ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc
    11821 atatccgctg gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct
    11881 tccataggaa tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt
    11941 gatttcagtt tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg
    12001 ctgacgataa ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct
    12061 gctaatacat attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat
    12121 ggcgtgatgt atgctttcaa tatttctaca atgaatggtg tttttttgcc atatgaaatc
    12181 gcattttcac cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct
    12241 gatgcatgga atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt
    12301 ttttgtgtat ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt
    12361 tctcgctgct tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc
    12421 tgagaacaga tagataatag ccctggcctt tcctctagat ccttgttttt cgaatgactg
    12481 tcatccattt gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt
    12541 ttcgaatagt tatttttcca attagaccac ctccttttta tttttcaccg atggggattt
    12601 gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa
    12661 aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc
    12721 tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt
    12781 ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta
    12841 ttcggttttc cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg
    12901 tttttcatac tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt
    12961 ctcattttcc acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa
    13021 agaacacttg ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatc
    13081 caacgttttg aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct
    13141 gaattagcaa aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc
    13201 gattacatga cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat
    13261 atcgtccgta agatggttgg agcaacaaaa gctgccgatg ctgttccagg tacgatccga
    13321 ggagactacg cgctgccggg gactgaaaac atcattcacg cttctgattc ccgtgatgct
    13381 gcggtaaaag agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa
    13441 gcggcgaagt gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag
    13501 cagttttttc tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa
    13561 gaattcttgg tagtctttcg tcttttcttc ccctaaagca gcagaaatca atctttgata
    13621 ataatctgcc atcaatgttt catagatcac tgtacttctt tctttatgtg ctaagctccg
    13681 tttcatatct tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga
    13741 agtcaggttc attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg
    13801 tcgcattctt tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca
    13861 aggaaaatca gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta
    13921 ttcttcaatg attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt
    13981 tttttcgacc atgtgaagat tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg
    14041 cctacggtta ttgctgatct ctttataagg atcaaagctg tcttcttgta cccgatctag
    14101 cattcgtctt tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata
    14161 ttctgcaagt gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt
    14221 tttcactgaa tttgtctcaa tcccgactag agaagtctta aaacagattc cttccgctaa
    14281 ttgggactgt gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat
    14341 aatagttctc ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg
    14401 aaaaatacac gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca
    14461 ttagaatgtt gtctaactac tgaaggttgg tttgattgtt tattgattat ttttttgctg
    14521 ttcaacgtct gttcttgtta ctggatataa gttggttgtt gttttattac cattttttga
    14581 aaagacactt gtcgaatgtt cgcctttttg cttttcgatt gcttcattac gttccgcttc
    14641 atttttatag tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg
    14701 caagagattt tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg
    14761 atctttggag atttcctcat cgattcctgc tgctttcgta tcgatgattt ctcctgtctg
    14821 attattgtaa gcttttcctc ctaggacggt gtatttgggt gtcacaaagt tcccatttcg
    14881 aaaagcaact acttgatcat gctgagaaga taataagtct gtaccaaaat gaagataatc
    14941 tttatcatcg attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta
    15001 ttcatgattg actgtccctt tagtataacc aggcatatgg atcatcaatg gcactcgttg
    15061 catctgtgcg ttgtcaaatt catcccatgt atctggatct ttaccaagag cgcttgctaa
    15121 atctttatta tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt
    15181 ttggtaaata ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata
    15241 atgtgccgtt cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc
    15301 gaaattcttg tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta
    15361 aaacggctgt tgtagatgct caagatattg ggcagattct ctgaataaat ctttatctaa
    15421 tatcccataa cctaacgttt cgtcggattc atcaaaataa gatcgatcaa agaaattgtc
    15481 ataacctaag tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa
    15541 aacagcgctt gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt
    15601 gtctgaccca agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa
    15661 catattttca gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaagct
    15721 gatcgttgct tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac
    15781 cttcatatta atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc
    15841 tttagcgatc ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa
    15901 aacttcatca atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt
    15961 gattccgtca taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc
    16021 aaatgttcgc tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa
    16081 aataagccct gaaagacagg taacagcgat agccatcggt ttgccgacag ggttgctttt
    16141 gataggtatt ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccag
    16201 ccagtagatg acatcatggg gcttcatcaa agaaaaggag cttcctgaca atccttgcga
    16261 cactttcgaa aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa
    16321 aataatattc aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatagctaa
    16381 cgctggcttt ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat
    16441 tgggttgatc cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc
    16501 atagtaggca atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa
    16561 acgattttgg cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt
    16621 tttcttattt tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt
    16681 tttattcttt ctaaagatta ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa
    16741 gcgattaaac taatgaagag ataaaagtct tgtggtgacc gcttcactcc acggtattca
    16801 aaagccagtt tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc
    16861 tccttatttc ttgatgcagg acagcttttt cacaacttct tccttaaacg gtgttctcct
    16921 gccaactcct cggccacaag tcacaatatt tataaaacat gagaagctgt tttttgaaat
    16981 tgttccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa
    17041 tcagctgacc ctcggtatta gattatgtca ggacgctaat tttacaacct cttggtaaac
    17101 ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct
    17161 attttttgaa attgctcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata
    17221 cacggtgttc aatcagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
    17281 tattttgccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctgttt
    17341 ttcattgtcc ataggattga tattgtcctt gtgatgactc attcgtaata attgaggctt
    17401 gattttcttc acttcccgtt atcgtttgtc cactgccaga atctgaagga cttgttcctg
    17461 tcgactcgtt tttttgagaa gagacagttg ttttcttgac ttgttcattt gtctttttcc
    17521 attccgctaa acgatcggcg accacttttt tgtctacttt ttcagaataa gcggtgatca
    17581 cttttccatt tttcaaacgg gctactttgt cgccatcatg agtcaaaagc aaaacggtca
    17641 aatgcttgtc tttaaattga tattgtctga gagaaatcgc actaagtacg tcatcgatat
    17701 taataaattg attttcttct tttccttttg gcaaataaat gtccaattcg cctttttcgt
    17761 aattggcagt tattttatac tccgcccacc aataagttga gaattctttt ttgaaattat
    17821 ctactgattc tttgcttgct acgatttttt ctgtttcttt ctgagcaaga tctgctgtca
    17881 tttttcctga aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga
    17941 gaagaaacgg tctttttttc ctcatgcttt cctccactcc tatttaattt tgactattct
    18001 attatataca aaaaatgaaa tagaaagaca aaaaagttga aaaagatcgt aaataattga
    18061 acttcttttt cacaaaatgg taagaatgga gaatttcacg catttgctct caactattct
    18121 ttcttaagcc tcaaatccgt ttttatctga tacgtctttg aaccaatgac ccgatttttt
    18181 gattgttctt tttccatctt gtgctaagtc tactgaaata aagccatatc gattcttata
    18241 tgcattggtc cacgaccagt tatccataca tgtccacata tgatagccta aacaattggc
    18301 accttcttga atggcttgat ggacatattt cagatgagat tggacaaatt cgatccgata
    18361 atcatcgtgg atcatgccat cttctttgat aaatcgtgct tcgttttcta cacccatacc
    18421 gttttcagaa ataaagcagt cgatatttcc ataatgatct cgtacattga tcaaaatatc
    18481 atagattcct tgttcataga tctcccatcc gcgataagga ttcattttac gaccaggcat
    18541 ttcataattg tcgaaataat cctcaggcat tggaccgttt ttagtatcga ttggtgtttc
    18601 ttttgctttt acccggcgtg gctggtaata attgatacct aaaagatcta ctgtattttc
    18661 tttgataacc gtcaaatcct ctgcatggat cacaggcatg agatcaagtt cttttactat
    18721 ctcaatcaat tcacttggaa actcgccttt tacagctgga tcaaggaatg aacgattgaa
    18781 gaacgcatct gctatttgcg ctgctttaac atcttcaggg tttgattcgt ctcttggata
    18841 gcttggtgtc agattcaaga tgataccaat aacaccacct tgctccattt catggtaaga
    18901 tttgatggct aacgctgaag ccaacgcttc atgatagcca acttggatcg cttttttcat
    18961 atctgactct tctggataat gccatcgata taaataacct gcttcgactg gaacgattgg
    19021 ttcattatga gtaaaccatt tttttacttt cgaaccaaac aattcaaaac aacttttggc
    19081 atatttttca taggcttcaa ccgtttctcg gttcaaccat ccgccttttt cttgcagata
    19141 catcggcata tcgaaatgat acagattcac aaagggttcg ataccgtttt caatcaactc
    19201 atcaaaataa cgatgataga aacgaacggc ttcttcgttg attgttccag attcatcagg
    19261 tatcaaacgg ctccattgga tagatgttcg aaatgaatta tgcccagtct gtttcatcaa
    19321 ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc
    19381 aaagaacttt tgcggttctt ttttgtacca ataatcaaaa atgttctctc cttttccatc
    19441 gcctgaaatc cttccttctg tttgtgggcc acttgctgca gaaccccacc aaaaatttgc
    19501 tggaaattga tattttttca tcaaagcacc tctttttctt ttttagttcg ttttagttga
    19561 ttccatgtat gatattttcc atcacaatca ctttttcatt ttcttcctct tcttttttga
    19621 ttcgttttaa cagctgttct gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg
    19681 tgattttcgg aaggatactc agatcgtttt tatctaaata atcatagtga ctgaacgcaa
    19741 tatccttccc gattctgaca ccttcttgat tgaaacattc gatggcacca atcgacatat
    19801 tgtaattgct gacaaaaata gcagttgccc ctttttctaa taatgctttt gccccgtaat
    19861 agccgctttt tctagaatag tctcctgtaa agacctctaa ttggatcgat ggatcatttt
    19921 ggaccgcttc tttggctcct gctaaacgtt ctcttgctac gtagtctgtt tgagtggcag
    19981 caataaatcc gattttttta tgtccttgtt caccatgatg agcgatcact tttgctacac
    20041 tttctcgatc attcactaat attgtatcaa tacctgctgg tccttctgga caattcaatg
    20101 cgataactgg aatactcatt tttgtgagca tttcaaatcc agaccactct tcttcggata
    20161 agaagacaat cagcccatcg atcacatgct tcatcaggta ttcaattttt tgttcaattt
    20221 tttctggctg atcggcaaac ccgcttaata ataagctata cccattttct tcagctactc
    20281 tttcgatccc tgagacaact tccgacccaa aacggctgga aatcccgttt atcaaaagtc
    20341 caatagagaa gcttcgatta ttcttcaacc ctttagcaat gatgttttct ttatagtcca
    20401 acgctgaaat cgcttctgct atttttttca tgttcttttc cttcagttgc tgaccattca
    20461 agtaacggga aactgttccc actgaaacac cagccatctt agccacatca cggatcgatg
    20521 ccatacattc tcactcctgc cagttacatt tgatttctct ttcttgttgt tcgattatga
    20581 tgaccgcatt ctttgcatag tgttgcttgt catcaatatc catataacta aagtcttcag
    20641 tagagcaact tcctctcact attaattata cttaaacaag tatttgaacc ggttcatttt
    20701 taatatagca tttttttaaa agattgcaac acttttttaa aacgctttct gaaagtggcg
    20761 gattttttgc ctttttaaaa ttttattcta aaatttactc tatttgtggg tgttttgtgc
    20821 gtttttctct tccatccgtt tgaagtttta cattccaatg gtataatttc tttatcctat
    20881 caaatggggg aagtattttg aagaaattaa tcgtactatt tattttgtct gcgggacttt
    20941 tgacattgac tggctgtgat ttgtttggcg gctctgacca atcctcggac gaaacaaccg
    21001 attcttctgt cttaaaagcg gaaaaagcag ataccaccaa cagtgtatat acgctaaaag
    21061 tacgtaaaga tatggattta tccgcgggac agttgacatt tacagataaa aaaatggaat
    21121 ggacacgcac gtatcagaca caaggaactg attcttctga atcatctgaa gaaaaaacag
    21181 tgctgacaga tatcaagatc actaccaaag atgacgaata tattatttca ggaaaagaaa
    21241 ataaaaaaga aacaacagtc atatttacaa aaatcggtaa ttaccgtttg aaggatgagg
    21301 atggcaacat ctacagtcta tagtgccgtc tgttggatct tcttttgttt ctccccttct
    21361 tttctgctat agtaaagaag caagatgatt gtaaaggaga cgaaatggat gatcgatagt
    21421 cctgattatt gtacatgtaa taacaacaaa aatatccata cagaatttaa tgaccaagga
    21481 agcttgagca tatgcgacaa ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa
    21541 tcttatcctg ttcaaaatta tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact
    21601 cactatggct gatttttatg aacttcaaac tcagcttctt cctttgatca agcttcatca
    21661 gtaaaacaaa cagtggatca tttcagttta caaatcaaag agtaaagatt tatttatttc
    21721 aaatatttga atataaaata accaaacgat agaaaggctt attctacatc acgtagtaat
    21781 ttttctttcc cgtcgtttgg ttttttattt ttagcctaat tcttgacctt ttgcatgctg
    21841 gttatccgta aaatattttc cagcaggctt tccttccaat tcagctaatt tttcttggat
    21901 ctgacggatc accttttttg catcttctgg attttctaca aaatcgaatt tatccccatc
    21961 cacttgtatt ttaggacata catcaaaatc ttcataccat tgattatagc gggtgttcaa
    22021 cgtttcataa taggtatata acgtctcatc gctttctagc tgttcatagt cacgcccacg
    22081 ttttttgata cgttctagca tcgtgtccaa agaaactttg atatggacca tcaaatcagg
    22141 ccgttttttt ggagcaacat catctaactc gtttagcatc gtatctaata agctatcgta
    22201 ctgctgtact tcgatatccg ttacccgacc taaatcagcg tttagatgga acaacaggct
    22261 atcttcatag atggatcggt ctagcacatt gtcatcgttc actaaagcat ttttcattgc
    22321 taaaaatcgc ttgttcaaaa agaagatttg caataaaaaa gcgtactgct caggatttga
    22381 ataaaagagc ggcaatactt cgttatcttc gacattttca taaaatggtt tagaatcaat
    22441 ctcttttgca agcatatccg tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa
    22501 aatcactgac atacaaccct tcctcttcta taagtactcg cctacgtaag tttcaacagg
    22561 caatgtttca aaatcatatt cacgtacagc ttggtctgat tttaaatacg tatggagact
    22621 ttgtgtaatc atcaagttcg taggtaccgt ctcttcttgt acaatgatca tcggtttttt
    22681 caacatagtt gccacaccta attcgtaagc cgttcctgga tcgattgttt gatgctcaaa
    22741 gtcaaggata gcaacaacaa attctgcatt tgtcagttct tccatatctt tttcgtatac
    22801 ttcctgcgcc catccagcgc tgaatagttc ataattgctt tcttggtgat gacgtggaga
    22861 gtagaaactt gtgactgttt tgttttcctc caacgctttt tcgatacgac ttactcgatc
    22921 gatttgctcc tctgaaaaaa atggtcctgc taaatatatt tgactcatta ttttgcctcc
    22981 ttcaataata actgattttt aatagatact gctcgtaatt cattgaccag ttccaatgga
    23041 ttgttggaaa agattacttg atccaaccat tttttatcca taaaatcctc ttttgcaaaa
    23101 gtttctaata attgttgcaa gccgctgtaa taaccgccaa tattcaatat tccgattggc
    23161 ttgtcgggcg tcagaccgat tgctatccaa gaaagaagtt gtgagaattc ctccaatgta
    23221 cctagacctc cagggaaaac gaggacagca tctccctttt caaataataa tacctttctt
    23281 tcatcgatcg tttctgtagg aataaatgta gtaacatctt ttccaggcaa ttcagtttca
    23341 aaaagcccat tcggatagat acctgtgact tttccgttat tttccagtac tgttttacta
    23401 atcactccca taatcccgga gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc
    23461 atgtattttc ctaacgtttg cgcaataaat ttgtaacttt ctttattacc aaaacgggaa
    23521 ccgcaaaaaa cagtaagttt cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt
    23581 ctacacgtgt ttttttaaaa taaaagacta gattttgtag ttttttagtg gtat
//
LOCUS       DARBER010000028        23240 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARBER010000028 DARBER010000000
VERSION     DARBER010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.028"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..666
                     /locus_tag="SCK06_001119"
     CDS             <1..666
                     /locus_tag="SCK06_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1151136.1"
                     /translation="ILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISY
                     FVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASV
                     MGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPR
                     NSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDS
                     ALF"
     gene            678..1598
                     /locus_tag="SCK06_001120"
     CDS             678..1598
                     /locus_tag="SCK06_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1151137.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1639..3096
                     /locus_tag="SCK06_001121"
     CDS             1639..3096
                     /locus_tag="SCK06_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1151138.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3441..4055
                     /locus_tag="SCK06_001122"
     CDS             3441..4055
                     /locus_tag="SCK06_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1151139.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4048..5772
                     /locus_tag="SCK06_001123"
     CDS             4048..5772
                     /locus_tag="SCK06_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1151140.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5769..7220
                     /locus_tag="SCK06_001124"
     CDS             5769..7220
                     /locus_tag="SCK06_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1151141.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7693..9846)
                     /locus_tag="SCK06_001125"
     CDS             complement(7693..9846)
                     /locus_tag="SCK06_001125"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG1151142.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9848..10894)
                     /locus_tag="SCK06_001126"
     CDS             complement(9848..10894)
                     /locus_tag="SCK06_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1151143.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11044..12333
                     /locus_tag="SCK06_001127"
     CDS             11044..12333
                     /locus_tag="SCK06_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG1151144.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12346..15048
                     /locus_tag="SCK06_001128"
     CDS             12346..15048
                     /locus_tag="SCK06_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1151145.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15033..15890
                     /locus_tag="SCK06_001129"
     CDS             15033..15890
                     /locus_tag="SCK06_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1151146.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15961..16860)
                     /locus_tag="SCK06_001130"
     CDS             complement(15961..16860)
                     /locus_tag="SCK06_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1151147.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            17029..19200
                     /locus_tag="SCK06_001131"
     CDS             17029..19200
                     /locus_tag="SCK06_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1151148.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19268..19609
                     /locus_tag="SCK06_001132"
     CDS             19268..19609
                     /locus_tag="SCK06_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1151149.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19885..21639)
                     /locus_tag="SCK06_001133"
     CDS             complement(19885..21639)
                     /locus_tag="SCK06_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151150.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21650..22486)
                     /locus_tag="SCK06_001134"
     CDS             complement(21650..22486)
                     /locus_tag="SCK06_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1151151.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22488..23171)
                     /locus_tag="SCK06_001135"
     CDS             complement(22488..23171)
                     /locus_tag="SCK06_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG1151152.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7639 a         4156 c         4782 g         6663 t
ORIGIN      
        1 atccttttga atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat
       61 aaacgactcg ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc
      121 attatcagtt actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg
      181 atcactggac atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt
      241 atcttggtgt tcattggtac atggaaaggg attggttata acagcattat ttactttgca
      301 tctgttatgg gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa
      361 tggcagcaga tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg
      421 attttagccg taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg
      481 aattccggtg cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg
      541 acatccacag gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc
      601 tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta
      661 ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca
      721 ataagacggc taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt
      781 tgccatttat atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg
      841 ggtacagttt ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga
      901 tgcaggataa aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac
      961 tgatcaatag cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat
     1021 tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg
     1081 ccaattatct tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct
     1141 tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg
     1201 tctctgattc gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg
     1261 ttcagatcgt tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat
     1321 tgggttactg gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc
     1381 cactacagta tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag
     1441 gagcaggaag ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg
     1501 cgatcgtagt catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt
     1561 ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata
     1621 aaattaggag gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg
     1681 ttgttgttgg gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct
     1741 agcaattcgg acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa
     1801 ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat
     1861 tacattggct ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat
     1921 tatgatatcg cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat
     1981 ttgactgaat tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat
     2041 attaaaggaa atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt
     2101 gctcaacaag tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat
     2161 ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca
     2221 aacacggcag cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct
     2281 ttagggaata cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac
     2341 caatacgata atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa
     2401 ggactcattc caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact
     2461 tggctaatgc gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat
     2521 gcagcaaaac aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa
     2581 gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa
     2641 cttctagggc tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag
     2701 ggagaagcat gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat
     2761 cagccaaata cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa
     2821 gagtctatta cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact
     2881 tcaccgattt taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt
     2941 tctaatgtca tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa
     3001 acattaccga aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa
     3061 atgcaaaaac aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg
     3121 tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct
     3181 cggtattaag ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac
     3241 tactcgaaac taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctaacc
     3301 ctcggtattc gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta
     3361 ataatcaggt caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact
     3421 taatgtttgg aggagaagat atgttaggga aggttttgga agagttattt atccggatct
     3481 gggtcattat aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct
     3541 ttggattagg tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt
     3601 ataaagaaat cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca
     3661 atctgctatt tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat
     3721 ctgttcaaat caaagggctc atttttttag ctattgattt tattttactt tttgccttgc
     3781 tctgcctatt tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg
     3841 ttcctaatct gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa
     3901 aaataattat tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc
     3961 tgtttggtgt cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg
     4021 aagaattaga taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga
     4081 accttactaa atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact
     4141 gctaccgtta tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg
     4201 acaagtgatg ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg
     4261 atcatcaaaa accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta
     4321 acaaaaccca tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa
     4381 ttagtttctt tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg
     4441 atatatatcg tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt
     4501 ggggaaataa aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc
     4561 acacaaggag gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat
     4621 atgattttag aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg
     4681 acaaatcgac tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa
     4741 gaaatgatca tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa
     4801 aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta
     4861 gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc
     4921 attattttag acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag
     4981 caagtatcta tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg
     5041 atcgacataa caaacacgga atatgaactg agggaacttt caatcggtat caacgaaatg
     5101 ttggatagca tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac
     5161 gcacatatgc gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa
     5221 tatatccgaa tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg
     5281 ttttccgctt tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa
     5341 ctggactttt gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc
     5401 tatcatttta tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag
     5461 ccattagttg aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta
     5521 agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa
     5581 gggatgactg aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa
     5641 cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc
     5701 agttattata tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc
     5761 aaggagggat gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg
     5821 ggttaaaaaa gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga
     5881 atgccaaaga aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg
     5941 tgacaatgcc agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata
     6001 attttgaatt tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc
     6061 aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc
     6121 tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct
     6181 atcaagaact gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag
     6241 aaataatagg acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa
     6301 ctcatgatac tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac
     6361 gaaattacgg agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct
     6421 tctgttgttt tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag
     6481 agacagattc tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt
     6541 tgcatcaatt ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa
     6601 aagatcaaac catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattggaag
     6661 tcgctcaaaa aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg
     6721 aagatatacg atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc
     6781 tggaagatga ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag
     6841 aacttcagca gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg
     6901 acaatgtgga aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca
     6961 aagaagtgtc agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag
     7021 aaacgaaaaa aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac
     7081 tgcttttgca cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca
     7141 ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag
     7201 aaaacagtgg agaatcataa aagaaaaagc caatgtaatg aaataactta taaaaaattg
     7261 tctagaaatc taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc
     7321 tctcgataaa cggagttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
     7381 gtggaaagcc attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacga
     7441 cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaaa aaatggcaaa
     7501 atatgaggaa ctattttgcc atttgtctct ctaatactca ggtcagaacg ctgatttcac
     7561 aacctctcaa taaacggtgt tcaaaagctt gcatctgcgg taataagacc aaccaaacga
     7621 aaaatatgag gatctatttt tgcttggctg ttcttattac ttgatgcagg acagcttttt
     7681 cacaacctct tgttaattat agtttgataa ggaaaaaggc aaatcttttg cttgatattt
     7741 cttagggtga gggatgatcc ctaattggta agagatcgtg ccgcctttca gcaaatcttg
     7801 atgggtaaaa tataatttcg tatattctct gtccatccgt ttgattttat ggataaattg
     7861 ttgctgcggc cgattaggcg ttgcttcaat cgtcaattcc tttccatttg acaaaagcaa
     7921 aacagccttg tcaattaatg gcataccgat aacatattca ccggttccag gcgtgacagg
     7981 ataaaatccc agactattga agatatacca agcggccata cttccattgt cctcatctcc
     8041 agggtaaccc attggagaat cgtcgaaggt ctgagtcatc aactgtttaa gcagtggctg
     8101 tgccatttca ggttgtccta tataactgaa caagtaagga tagtgaaaac ttggctggtt
     8161 tgaaatagct aattgtccaa attctatcgc ggccatctca ctcatttcgt ggatctcaaa
     8221 accatatcct cctgggtcaa acgttggggg ctgattgcac agctcaatga gtttttcttt
     8281 atatctctca ctgccaccat attttccgat cagtcctgaa aaatcgtgat aaacagcaaa
     8341 gctgctttgc caagcacttc cttctgcata atcttgtccc caacgattag ctgaaaatgg
     8401 ttctctgaac ttgccatctt tatcttttga acgcataaat cctgactcat agtcaaagat
     8461 attttgatag ttcaatgatt gctgctgata tttcttcgtc atttcctctt cttctagtac
     8521 cttggcaact tggctaatac aaaaatcgct ataagcatag tctaatgtgt gattgactga
     8581 ttcgtggtag tgattaggaa catagccata tgtcaagtaa tcttctgttc cttggcgtcc
     8641 ataatttgga tgtttgcttt gaaccgtagc acctttttcc attgccgtta gaaattcagg
     8701 catcaagtct cgtcggatat cttttacagc agcatctgca atgaccgcat cgatcaatgt
     8761 cccaggcata agtccacgtt catcaggaga cagccatttt ggcaaatagc cactttctct
     8821 atagctgttc aaaaaacctt ccagcatttc ttcgtatttt tctacagcaa tcaaagaata
     8881 aagaggataa accgttttat acgtatccca aaacccatta tttgtataga gaacaccagg
     8941 ttttatagat cgactagtcg tatcatagtg gattttttgc ccactaggat caatttcata
     9001 aaatgtttgt ggaaataaaa acacccgata taaattatga taaaacgtcc gcaattgttg
     9061 ttgatcatgg tgtgtgagct gtatacgatc aaaataggac tgccattgtt cttgactgtg
     9121 agcaaggtaa gtctctttgc tttggttttg ttctagttcc aggttataag ctgcttgttc
     9181 cgagctgata aaagatgtcc caaatcggat agtttgtttt tttgtctgtt caaaatacaa
     9241 cgggatcgag tcgctatttt tgttggaaac agctaactca tcaattgtta aaggatattc
     9301 aaatctcaat ataaaataaa aagaaaaatc aggatcttct gaaccagcaa aatgtgtgat
     9361 ttttccttcc actgtgtaag gatctataac aaagagctga tggcgtcccg gaaaggataa
     9421 aagtaaaccg ctgtcatttt tgctgtaatc aatagataag attccgccat acatgctagg
     9481 tatcaatgta gaacagatgc cataacgtaa ttgacagatc gacagatgag tcgggcaaaa
     9541 tatactttct tctgggcggt aagaactttg agcatggaaa agggtgttct cctgtagttt
     9601 tccattgatc ggcgtaaaca gcatatgact gaaatcaccc atccaaggac ttggttgatg
     9661 ggtaagacgg tagccttgaa aaacgcggtc atcaggatga aaccaccagc tgccgttttg
     9721 atccgtagtt tgcggtgcaa aataattcat cccaaaagga acgccagtat aaggctggca
     9781 gttcccatta gagaaatttg gctgattcgc tgttgcatgt ctagtatcta tttgaccgat
     9841 attcatttta tttctccttt gttgattgtc ttttgatgaa tgaaactgga aatttactat
     9901 cggatggtcg atcttttgtc tctaaccaag tgaccaaagc atctcccgca agcttaccta
     9961 actgttcaaa atcttgtgcg atggttgtca gtggcgggtc aacaagagct gctgcttgga
    10021 tatcatcaaa tccgataatt gaaaaatctt cagggatatg ataaccatgc tgtctcaaga
    10081 gattcatggt ctggatagcg acaacatcgt tttcacaaac aaaagcagtt acttcatact
    10141 tagtgatata agaaagcaat tcatctaatg tcgcgctttt gtcatccagt gaagtgtgga
    10201 aagtcaaacc agctttgtcc aatgcttcaa ggtaaccaag gtaacgctgt cgaacggatt
    10261 gtggatgagt ctgcgtacca aacaaataag cgatttttga atgtccctgt tcgatcaaat
    10321 aagaggcagc aagcgcaccg ccttggaaat tatcggaaag aacggtagga aaaggtaact
    10381 catagatttt tttatctaat atgacgacag ggaattcttt caatgaaagc tcaaaaagta
    10441 agtcaagatg ttgttctgta tcaagagcat aataaatcaa tccgtcaaat tcattgatta
    10501 gctcttttgc tgttttattt tgtagaaagt caagcgtcgt catcatgaca tcaatctgtt
    10561 tatcttgcat cacagggttc aatcccttgc taaaatcacc aacagacaaa tcagcgacat
    10621 atggaagaag aaacagaatc cgtcgtgttt ttgtaggaga agcaagaatc tgttccggag
    10681 aagcggattt aacgaaactg cctttcccgc gaacgcgata aataagtcct aattgctcta
    10741 attcagtcaa tgcacgtttg gaagtgattc tgcttacttt ataggtacgt gaaagttctt
    10801 tttcagttgg tagttggctg tctacaggta attcaccagt taaaatagcc gtttgcaaat
    10861 cattaaatat tgtctggtac aatggttgag tcatataaac acctcttaat gatatatcat
    10921 attcaaataa taccataatg gatgaaaaag taaataaaaa gatatttttt gaaagcgtag
    10981 acataattgg ctgtatgaag tataatcttt aaagatatat caaatagaaa gaaggtttta
    11041 ttagtggttt ataaagatat accaaaatcg gtgcagaaat ttatggaaga tatcacacaa
    11101 aaatgtgggg atatccatgc ggactgggca aagaatttta cagcagcatt tgccaatact
    11161 ctgttgacaa cagtcaaaaa gcaaaatgac ggaaccacct ttttattgac gggagacatt
    11221 ccagcgatgt ggttaagaga ctctactgca caagttcgtc cttatttagt gattgcaaaa
    11281 gaagatgagg aattagctga catgatcagc gggttagtca aaaagcaatt tttttatatc
    11341 aatatcgatc catatgcgaa tgccttcaac gaagaagcta acggtgctgg ccaccaaaca
    11401 gatcatacaa aaatgaacga ttggatctgg gaaagaaaat atgaaattga ttctttatgc
    11461 tacccaatac agttggccta tcttctttac aaaaatacag gaaaaactga tcaattcaat
    11521 caatctttta ttgaaggtat caaaaaaatc ttggacgtgt tcaaaacaga gcaagatcat
    11581 gaacactctc cttatacatt tgtacgagac acgacaagaa aagaagatac gctgatcaat
    11641 gacgggcgag gtgcaaaaac agcttttaca ggaatgacat ggtctggttt ccgtcctagc
    11701 gatgatgctt gccgttatgg atatctggta ccatccaata tgtttgcggt ggtcgtgtta
    11761 ggttatgtgg aagaaatttt tcaaacgata ctcgatgata aagaaattgt acagcaaaca
    11821 cagaaattga agaaagagat ccaagagggg attcagaatc acggacgaac ggttaatcaa
    11881 aaagggcaaa cgatttatgc atatgaagta gatggaatgg gaaacgccac agtcatggat
    11941 gacagtaatg tgcccaatct gatcgccgca ccttacttgg gatattgttc tgaacaagag
    12001 gaagattatc tgcaaacaag aagaacattg ttgagtaaag aaaatccata tttctatgaa
    12061 ggaaagtacg caaaaggaat tggatcctcc catacgccag aaaaatacgt ttggccgatt
    12121 gcattagcta tggaaggaat gacaacgtca gacaaatctg aaaaagagcg gatattgaat
    12181 ttacttgtag caactgacgc aggaacacac cttatgcacg aaggattcga tgtgaacaat
    12241 cctgaaaatt atacgagaga atggttttct tgggcaaata tgatgttttg cgaattagtc
    12301 atggattact ttgatattag agtagaaaag taggaggtat cgtaaatgaa aaagaaagta
    12361 tatatcattt ctcatagcca ctgggatcgt gaatggtaca tggcttacga acagcatcac
    12421 atgcgactga ttgaattgat agacgatcta ctagagctat ttgaagttga tccgtccttc
    12481 aacagttttc atttggatgg gcaaacgatt attttagatg actatcttca agtaaggcca
    12541 gaaaaaagac aggcaatcca acaagcaatc aatgaaggaa aattgagaat tggaccattt
    12601 tatatcttgc aagatgattt tcttatcagc tcggaatcga acgtgagaaa tatgctgatt
    12661 ggcatggaag aaagccgtaa atggggaacc cctgtcatgt taggctattt tcccgataca
    12721 tttggaaata tgggacagac gcctcaatta atgaaacaag ctggtatttc agcagcagcg
    12781 tttggccgtg gtgtaaaacc aattggcttt gataatcaag ttttggaagc agaaaactat
    12841 tcttcccaat actcagagat gtggtggaaa ggccctgatc aaacagcaat ctttggtctg
    12901 ctatttgcca attggtatag caacgggaat gagattccag tagaaaaaga agcagctctt
    12961 gccttttgga agcaaaaatt agcggatgca gaacaatatg cctctacgaa ccacttattg
    13021 atgatgaatg gtgttgatca tcaaccggta caaaaagata tatcaaaagc aatccatctc
    13081 gcaaatgaac tgtttccaga ttacgagttt attcattcta attttactga ctatctggaa
    13141 gcagttcaaa aagatgtgcc agaagactta ggctcagtag aaggagaatt gacaagtcaa
    13201 gaaacggatg gctggtatac tttagccaat acagcatctg ctcgggttta cttaaaacaa
    13261 tggaatacca aggtccagcg acagctagaa aatattacag aaccacttgc gacgatggct
    13321 tatgaagtta gtggaaacta tccccatgat caattagatt atgcttggaa gacattaatg
    13381 caaaatcatc ctcacgacag tatttgcggt tgttcagtcg attctgtcca tcgggaaatg
    13441 atacctcgtt ttgaaaaagc agatgaagtt ggaaagtatc ttgcacaaga ttccttagaa
    13501 cagctgaccg cagcaatcga tacgaccgga ttcccaaaag acagctttcc atttgtcatt
    13561 gtaaatactg caggaatgga taaaactggt gaagcagaaa taaccatcga attagaaaga
    13621 aaaagattcg ctgaaggaat acctgaacaa ctatatcaag agttagaaaa tttgcctaaa
    13681 cgtaagtatc atgtagaaac aaaaagtggg gccactatac cagctatttt atctgaagaa
    13741 actgtccaat ttggttatga tttgccgaaa gaccgtttcc gtgtgccata tatggcaaga
    13801 atgatcaaag tgactttacc attagaaaac atgccagcat tttcttggga aacattcgcg
    13861 cttgtagaag gggaagcgaa agcagaagaa aaggaaacaa tgattcatca gagtggacga
    13921 atcatcgaaa acggccctct acatttgacg attgaaaaaa atggtaccat tacaatggaa
    13981 gatcgcaaaa acaaacgaaa gttgaataat ctgcatattt ttgaagatat cggcgatata
    14041 ggaaatgagt atatctttaa acaacctttc tgtgatcagc caatcctatc cagtaacaaa
    14101 gaaaactctg aagtaaaagt attagtagat acaccggaaa tagcaaaaat aagtattcta
    14161 caggaaatgg aaatccctgt ttctgcagac gagcgtttag aaaaagaaca gcagatggtt
    14221 gtggaatttc gttatcgaaa agcagaacga tccaaagaaa aacgaatcct tcaaataaaa
    14281 acaatcatga caatcagaaa agatagtaaa aaaatcgact ttgaaacgac tctcgacaat
    14341 cagatgaaag atcatcgctt acgtgtcttg tttcctacaa agttacatgt ggaacatcac
    14401 gaagcagata gtatttttga agtggtgaag cgcccaaacc atgtgtctaa aagttgggaa
    14461 aaccccacga atccgcaaca tcagcaagca tttgtcaata ttcatgatga agagtacggt
    14521 gtaacagtcg gaaactttgg tttgaatgaa tatgaagtaa cagaagacgg acaaatcgca
    14581 gtgacgctac ttcgcagtgt gggagaacta ggcgattggg gatattttcc aacaccagaa
    14641 gcacagtgtc taggtgagca tcggttcaat tacagcattg aactacatgg accagaagaa
    14701 aaattctcta cttatttgca tgcatacgca gcacagattc ctttttccac tcaacaaatc
    14761 aaacatcatg aaggaacatt gatttcaaaa caacaatatt taacgataaa aagtgaaacg
    14821 tttgcaatta cagctttgaa acgcagcaag tttagtgata aagtagtagt aagaggtttc
    14881 aatatgtcta gtcatttaga aaaattggaa ataactaaag acaatggaaa aactgtcata
    14941 ttgaatctgc ttgaagaacc aacgaagcaa gcggttgtcc caataatcca accttatgag
    15001 attcggacaa tcggattcga ggaggaaaac gaatgtatgg cggaatagaa gcaggcggaa
    15061 caaaatttgt gtgtgcggtt tcagatggtg gagaaatttt agaaaaatta agtatcccta
    15121 ccacaacacc agaagaaaca ttggatcagg tatttgaatt ttttgatcgt tatgaattga
    15181 agggaatcgg gattggctca ttcggcccga ttggtatcga caaaacaaaa gataattacg
    15241 gatacgtttt ggcaactccc aaaaaagggt gggcacaatt cgattttctt ggcacaatca
    15301 aggaacgcta caatattcct gctgcttgga caacggatgt caatgctgct gcatatgggg
    15361 aattgaagca aggagcagca caaggaaaaa atagttgtat ttaccttacc gtgggaacag
    15421 gaatcggtgc aggtgtcgtg atcaatgaag aaattttttc agggatcgca catcctgaaa
    15481 tgggacatat ttgggtgaaa cgccatccag atgaccatta tgaaggtact tgtccttatc
    15541 ataaagattg tttagaagga ttagcagcag gaccgtctat tgaggcacgc acagggatca
    15601 aagggcaaga tctgccgcaa gatcatcctg tttgggctat tcaggcttat tatattgctc
    15661 aagcactgat caactatacg ttgacgttag cacctgaaaa gattatttta ggcggtgggg
    15721 taatgaatca ggatcatctc cttcaaaaaa tccgtcagca atttgttgaa ttgatgggtg
    15781 gttatatgga aacacctcaa gtaagtgaat atatcgttcg ttggggactg ccgaatgaaa
    15841 gtgggattat cggtagtttg ctgttagcag aacacgaaca tcaaaagtaa gcagacaaat
    15901 tacacaaaaa gaacagagtc ttatcattat ctgctgataa gactctgttc ttttttattt
    15961 ctattcccat aatccaggta aagctttacc gactattttt tcttgagtaa atggatggat
    16021 catgtgcagt tctaatgcat ggagcatcag tcgttcagct gttctatttt ggtagagagg
    16081 gtctccaacg atcggatgtc ccttatgagc taaatgaact ctgatctggt gtgtacgtcc
    16141 ggtttccaaa acacaataga cttgtgtttg ttcagaaagt actttgttga cagtgacatg
    16201 tgtcaaggcg ctctgaccat ttttatcatc tacgaccctt tttctccgat catggcgatc
    16261 tcgtccaatt ttattggtga tggtcatatc tttggcaatt tctccccata cgatggcttg
    16321 atatctccga tagatcattt tttgttccaa cattcttcca agtatcggca agacaaatgg
    16381 attttttgca aataaaacgg caccgcttgt ttctttgtcc aagcggtgga caacgtaagg
    16441 tcgttgctgt ttagtgtcga gataagcagc taaatgattg agtaaggtac tgttttctgt
    16501 cggttcattt ggatgagtct tcattcctgc aggcttatcc actataatca aatgctcatc
    16561 ttcatataaa acacggatgt gcttcgcatc tcctaattga acttcttgat aattataatc
    16621 tgtttcttcg aataacaatg tgatctggtc attgcccttt acttcatgat ggaacaaagc
    16681 aggttgttga ttgatccaaa cgtttttacg tatacgcaaa aagtgtcgaa cctttctagg
    16741 aactaaccat tccttttcaa gcaagtcgcg aattgttgtt tcaggatggt tcataggtaa
    16801 agtgatcgtg attttcacaa gtgtactcct ttcagtgcaa ttgcagacta attgtaccat
    16861 ttttaaaaca tatttaagaa aaaacttaca tttttctgtt cattcttatg ttactctact
    16921 tgcactaacg taaaaaaaca tattgaacta caacttaagc aggaagaaaa cactctttca
    16981 ctatggtaaa ataagacgat tgaatcaaat agacaggaga agaagcaaat ggattttcaa
    17041 agcattttag aaaagataaa agctgctttg atcagttttt ggcgttggat cagaccatat
    17101 ctcggacaat tccatcgatg gcgaaagcgc atctggaaaa aatatcatgt gaacaaattg
    17161 attttgctga ttggtttgat ctgtgtctta gtgacaagta tctacctctt tattttagca
    17221 aaacaagcaa atgtcgaaac gttaaaatca ggattaagcc aatcgacagt catttatgat
    17281 aagaaaaacg aagaagctgg cacactgtac gcccaaaaag gaacatatgt agaattaaac
    17341 gcgatttctc cctatatcca agatgcagta atctcgacag aagataggaa tttttacgag
    17401 catcacgggt ttgatatcaa agggattgcc cgtgcagcag tccgaatggt cttgtctggc
    17461 ggaacatcag ggggcggagg aagtacgatc actcagcagc ttgccaaaaa tgcgtatctg
    17521 acattagacc aaacgtttga tcgaaaagcg aaggaattat ttttggcaat cgagatcgaa
    17581 aagaagtaca gcaaagaaga aatcttgacc atgtatctta acaatgccta ttttggcaac
    17641 ggggtatggg gtgtccaaga cgcttccaga aagtattttg gagtagatgc aacgaatgtt
    17701 acgctgtctg aggctgctac tttggcagga atgctgaaag gtcctggtat ttataatccg
    17761 atcgatcacc caaaaaatgc gaatgatcgg aaaaacacag tactaagtgt aatggaagaa
    17821 aacggaaaaa tcacaaaaga acaagaacaa gcagaaagcc agacagatat cagcgcgtat
    17881 cttaatgaca catacgataa ttcagattct ggttatcgtt atccttatta ttttgatgca
    17941 gtgatcgatg aagcagttaa cgagtatggt cttgatgaag aagatgtcat gaataaaggc
    18001 tataaaatct atacagcttt agatcaggat tatcaaaagc aattagaagc tacttatcaa
    18061 aacagtgcat tattcccagc aaatgcttca gatggtgcca tggtccaatc tgcttcagta
    18121 gctttagatc cgaatacagg tggtgtacaa gcactagtag gacgaagagg agatcatgtc
    18181 tttagaggat tcagctttgc tacacagatg cgtcgttcac caggatcaac gatcaaacca
    18241 ttaagcgtat atacaccagc actggaagct ggatacaagc cttccagtat cttgaaagac
    18301 gaaccgcaaa gttattacga tgcgcataat tttgacggga cataccaagg agaagtaccg
    18361 atgtatcaag ctgttgcaca aagtttgaat ttgcctgctg tatggctttt gcatgagatc
    18421 ggtttacaaa aaggatatga taaagcagaa gaattcggcc tgcctttagc aaaatctgat
    18481 aagtattatg gcttagccct tggtggactg gaaaaaggag tatctccatt gatcatggcg
    18541 ggtgcttaca gtgcatttgc caatgatggt cagatgtata cgccgcatct tatcacgaaa
    18601 atcgttgatt cgactggagc ggtgatcgtc gataacacaa acaaaaaacc aaaacaggtc
    18661 atatcaaagg aaacagccga tgaaatgacc agcatgcttc ttggtacatt ttcaaatgga
    18721 acagcggctg ctgcgaatcc tgcaggttat acgatagcag ggaaaacagg aaccacagaa
    18781 actaatttcg atgccacaaa agcaaatgac caatggatga tcggttacac gccagacgta
    18841 gtgatttcga catggatggg ttacgaagaa tccagcaagc tgcactattt ggaagggaca
    18901 agtggaaatg ttgtcgggaa agtcttcaaa tcagcggcag aaggcatcct tccgtatact
    18961 tcaaaaacag gatttagtgt agcagatgcc tatgccacag gaggacaagt ggtagctgca
    19021 gatcaagttg gaaacacagg aaattctaat ggagagtctg gaaattggca agataacttg
    19081 aatcaatatg gtgaacaagc aaaagaaggg ctgaaaaact tcggcgatat ggtgaaagaa
    19141 ggcgttcagg gaattggtga agcggctaaa gacctctggc ggaaatatca aggagagtag
    19201 cccctttgaa aacgaataac cccatgttac aatagagagt aactactaaa agaaaagagg
    19261 gcataacatg tcaaacatct acgatagcgc gaatcaaatc gaacgtgaga tccgtgaatt
    19321 accagaattc aaagcgttga aagaagcgta cgagaaagta caagcaaacg aagaagcaca
    19381 caaactgttc aaagacttcc aagcgatgca aatcgaatta caagaaaaac aaatgagtgg
    19441 acaagagttc agcgacgaag atgcaatgaa agcacaagaa atggctgtga aaatccaatc
    19501 agaagaagta atcaatgact tgatgcaaaa agagcaaggc ttcagtacaa tcatcaatga
    19561 cttgaaccgt atcatcatga caccagttcg cgacctttac agcgaataaa actattttat
    19621 gaaacaacag agggtgtgaa agagacgttc ggccttgagc gatgacaaaa ttgcttctta
    19681 ttgccgatag gctgtctctt gaacaccatt taccaagagg tcgtgagcct accgtttaac
    19741 tctgagttac aaggaacaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact
    19801 tgtgaccgag gagttggcag gagaacaccg tttattcaga aataggagct acgacaaaag
    19861 tcttgtcata gctcctattt tttgtcattc atttttcaag cggtagaaat agcctttttg
    19921 agctaacagt ttttcgtaag tacctatttc aacgattttt ccttcatcga tcacaacgat
    19981 tttatctgcc tctttgatcg ttgaaagccg atgagccaca ataaaagtag tgggtgtttc
    20041 tgtcaggtaa ttcagggctt gctggatttt tttctctgac tggttatcta aagctgaggt
    20101 tgcttcatct agcaaaatga tttttggctg acgtatcaac gctcgtgcaa tggagataca
    20161 ttgcttttgg ccaccagaca gcttgttgcc atgttcgcct actaaggtat ccagaccatc
    20221 aggaagctgg tcaatcagtg aagaaagttg cgcagcttcg atgacttcat ctaaacgttc
    20281 ttttgatacg tttttcaagc cataagtgat gttctctttg atcgaagcgg agaacaacaa
    20341 agtggtttga ggaacaacgg ctaaataatt tcgcacactt cgcatatcca gttcatccaa
    20401 aggctgatca tctagaaaaa tttttcctga agtcggctgg ataaatccaa tcaataagtt
    20461 aaggagtgta gattttccag agccagatgg cccaacaaaa gcaattgttt ctccagcttt
    20521 cacctctaga gaaaaatgat cgataatcgg tttgagtgag tccggataaa gaaaagaaac
    20581 atcctcaaaa cgaaaatttc catccaaatg ttggattttt ttcttaccgt gataagtttc
    20641 tacatcgcca gcaatcaaca gttcgccaat agaagtgata gattccatgc cttttgaaat
    20701 cgtaggaatc agcgtgatca ttccagaaac ggaattgacg atcgtcgtga aatacgtctg
    20761 atacatcacg acatcgccgg gttggatgcg cccttgtaaa gctaatatcc cagtgaaaat
    20821 caaacaaatc agctgaaata tctgaaaact agcccagctg actgatccga aaacagattg
    20881 caggatatct aagtggaatc ctttttcata gatttgagta aaatgcttat ttaagcgagt
    20941 tgtttcgtgt ttttctaacc catgagctcg agcaatcggt gctaattcga tcacttcgat
    21001 cacttttgca gaagtctctt ccatttcttg cctgaagtct gagttggact gtctgatcct
    21061 tttacggaaa aagagagtaa cgaatgcggc gagaggaaca gtcaataaga aaaacatgaa
    21121 aacaacagga cttttataaa gcgtgatacc taaagctacg aataaattga cagcgatatt
    21181 taatccgctg acaaatactt gagaggacaa cgtttggata gcttctacat cacggatgat
    21241 ctttgactgg attcgtccag attgcaattc tttttgataa ggaatagata gttcttggat
    21301 tttcttgata agggtcgctc ttagtccagc ttctacttga cggatcgaag tgctatggta
    21361 ttttacatga aaataattcg tcaatatatt ttgaccaatc agacaagcca tcaaaaaagc
    21421 gttgaaataa atggcctgca actgagaaga atctttcgat gaagctgcat tgattacatt
    21481 ggcaatagcg atcggcatta accaagatgg aagatgtttg ataataaaaa agagtcctga
    21541 catggcaaaa cgccaataat ttccctgata aagatgcagc aatgtctgaa aagcatttgc
    21601 atgatatttt tgataacttt tctgtattcg ttcctccata atcttctcct taacaatctt
    21661 ggtaaataaa ctgcaagtgt tctattgctt caggaacata aatctcagga gtagcttcaa
    21721 gagtggcgta cattagagga cgctcatatt tgatataatg cagtaccggg cggaaatcta
    21781 aacagccctt gccaacaggt accatgcgga tctttccatt ttccatgata tagtctttga
    21841 gatgaagcgc tgtaatataa ccatgtaaat tgtccaaggc acctcggatc actttttcct
    21901 gatcagcatg attttcttta tgcatcaaat tcgcacaatc taaaatgatt tttaaatttg
    21961 gtgaagctac ttcatcgatt aaacgtttag ctaactgata gttatagatt ggatgattga
    22021 tccctgcttc aacagcaaca gttacgccaa aattttctgc atgagcgact aattcaatga
    22081 tagaatttct ggcgatctga taagcttctt cagtaaaatt ttcttttgtg taaccgacag
    22141 agacactacc ggtttccgta gcaatcatct tggcttgata ggcagagcac agagcaattt
    22201 gctgtttgaa agttgccaat gcttgtgcac gaacttcttg atttttgctg gataggttga
    22261 tatagcatcc taaaatggaa agttcgatat tgtttttttg gaagtattca ccaaaatagg
    22321 cagcactccc aagtgtcatg tcagttaatt taagttgttc aggaaatgct ttttgtggtg
    22381 caaattggat atgggacaag tgaagctgtg atactttttg aacgatttct tcacgatttc
    22441 gaacatctaa atcgtgggca cggatgccta aatttaatgg aaacatctca aactcctttt
    22501 tgtaaataat tagcaagagg cagacgggat tcttttatca attcaacaat tattcgggca
    22561 actttatcag cgccttctgg actaaagtgg gtattgtctt ttagtccttc tgggtaattt
    22621 ttacaagtat caggcataag atgcaggtag aatttttttg tctcttcttt tgagaaggtc
    22681 tggaacaatt cttgtgatct tgagaacaca tctaaaagag gaatatggtg tttctcagca
    22741 aatgcacgca tcgcttttgg atagtctcca agtatatctg gattcaatgt tccgttttct
    22801 aagtaatctc gtcgcgtcac agatgtcagt aagaccattt ggacgttcgc tttttttgcg
    22861 cattcaaaaa atatctgcag attttttaaa tagctaccga agggaagcgt acctctttct
    22921 tcttggattt tctggtcgtt atgtccaaat tgtacaaata agaaatcatt cggctgaaac
    22981 tgtgaagcta gacgggctaa ccttccttcc tggataaatg atttggtgct gcgtccattc
    23041 tttgcttggt tattgatctg gatctcgggt gaaaaataag cagagatttt ttctccccaa
    23101 cctgtctcgg gacgcttgtt ttcatctttt tctgccgcag tagaatcacc ggcaatatgg
    23161 atagtaggca tgttatctct tctttcataa aggtttatag gcaatgaagc agaacagcaa
    23221 gagaagaaga cggagcaaat
//
LOCUS       DARBER010000029        23065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARBER010000029 DARBER010000000
VERSION     DARBER010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.029"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(111..566)
                     /locus_tag="SCK06_001136"
     CDS             complement(111..566)
                     /locus_tag="SCK06_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151153.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="SCK06_001137"
     CDS             complement(720..1928)
                     /locus_tag="SCK06_001137"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG1151154.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="SCK06_001138"
     CDS             complement(2040..2372)
                     /locus_tag="SCK06_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1151155.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="SCK06_001139"
     CDS             2520..3395
                     /locus_tag="SCK06_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1151156.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="SCK06_001140"
     CDS             complement(3477..4058)
                     /locus_tag="SCK06_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG1151157.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="SCK06_001141"
     CDS             complement(4064..5323)
                     /locus_tag="SCK06_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1151158.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="SCK06_001142"
     CDS             complement(5325..6371)
                     /locus_tag="SCK06_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151159.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="SCK06_001143"
     CDS             complement(6606..6881)
                     /locus_tag="SCK06_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG1151160.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCK06_001144"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCK06_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG1151161.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCK06_001145"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCK06_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG1151162.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="SCK06_001146"
     CDS             complement(9980..10660)
                     /locus_tag="SCK06_001146"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG1151163.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="SCK06_001147"
     CDS             complement(10709..11344)
                     /locus_tag="SCK06_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1151164.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="SCK06_001148"
     CDS             complement(11410..12813)
                     /locus_tag="SCK06_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG1151165.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="SCK06_001149"
     CDS             complement(12800..13831)
                     /locus_tag="SCK06_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1151166.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="SCK06_001150"
     CDS             13862..14083
                     /locus_tag="SCK06_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG1151167.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="SCK06_001151"
     CDS             complement(14248..14985)
                     /locus_tag="SCK06_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG1151168.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="SCK06_001152"
     CDS             complement(15138..16478)
                     /locus_tag="SCK06_001152"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG1151169.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="SCK06_001153"
     CDS             complement(16715..18238)
                     /locus_tag="SCK06_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG1151170.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="SCK06_001154"
     CDS             complement(18616..19227)
                     /locus_tag="SCK06_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1151171.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="SCK06_001155"
     CDS             complement(19557..20273)
                     /locus_tag="SCK06_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1151172.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCK06_001156"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCK06_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG1151173.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="SCK06_001157"
     CDS             complement(20832..21629)
                     /locus_tag="SCK06_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG1151174.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="SCK06_001158"
     CDS             complement(21599..21970)
                     /locus_tag="SCK06_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1151175.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCK06_001159"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCK06_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG1151176.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaa
//
LOCUS       DARBER010000030        22395 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARBER010000030 DARBER010000000
VERSION     DARBER010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.030"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SCK06_001160"
     CDS             8..649
                     /locus_tag="SCK06_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151177.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SCK06_001161"
     CDS             708..1148
                     /locus_tag="SCK06_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151178.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SCK06_001162"
     CDS             1160..1828
                     /locus_tag="SCK06_001162"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1151179.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SCK06_001163"
     CDS             1892..2515
                     /locus_tag="SCK06_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG1151180.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SCK06_001164"
     CDS             2571..2957
                     /locus_tag="SCK06_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG1151181.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SCK06_001165"
     CDS             3170..4561
                     /locus_tag="SCK06_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG1151182.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="SCK06_001166"
     CDS             5172..5540
                     /locus_tag="SCK06_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG1151183.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="SCK06_001167"
     CDS             5720..6661
                     /locus_tag="SCK06_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG1151184.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="SCK06_001168"
     CDS             6730..7461
                     /locus_tag="SCK06_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG1151185.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="SCK06_001169"
     CDS             7605..9785
                     /locus_tag="SCK06_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG1151186.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="SCK06_001170"
     CDS             9805..10287
                     /locus_tag="SCK06_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG1151187.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="SCK06_001171"
     CDS             10303..11064
                     /locus_tag="SCK06_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1151188.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="SCK06_001172"
     CDS             11195..12529
                     /locus_tag="SCK06_001172"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG1151189.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="SCK06_001173"
     CDS             12836..13321
                     /locus_tag="SCK06_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG1151190.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="SCK06_001174"
     CDS             13314..13997
                     /locus_tag="SCK06_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG1151191.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="SCK06_001175"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="SCK06_001175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG1151192.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="SCK06_001176"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="SCK06_001176"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG1151193.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="SCK06_001177"
     CDS             17613..18242
                     /locus_tag="SCK06_001177"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG1151194.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="SCK06_001178"
     CDS             18294..18626
                     /locus_tag="SCK06_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1151195.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="SCK06_001179"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="SCK06_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG1151196.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="SCK06_001180"
     CDS             19452..20825
                     /locus_tag="SCK06_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1151197.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="SCK06_001181"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="SCK06_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG1151198.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="SCK06_001182"
     CDS             21880..22320
                     /locus_tag="SCK06_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1151199.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7588 a         3872 c         4885 g         6050 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atggg
//
LOCUS       DARBER010000031        22124 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARBER010000031 DARBER010000000
VERSION     DARBER010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.031"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(159..1469)
                     /locus_tag="SCK06_001183"
     CDS             complement(159..1469)
                     /locus_tag="SCK06_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1151200.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="SCK06_001184"
     CDS             complement(1481..2899)
                     /locus_tag="SCK06_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1151201.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="SCK06_001185"
     CDS             complement(3139..4707)
                     /locus_tag="SCK06_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1151202.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..4992)
                     /locus_tag="SCK06_001186"
     CDS             complement(4711..4992)
                     /locus_tag="SCK06_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1151203.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4989..5912)
                     /locus_tag="SCK06_001187"
     CDS             complement(4989..5912)
                     /locus_tag="SCK06_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG1151204.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5909..6787)
                     /locus_tag="SCK06_001188"
     CDS             complement(5909..6787)
                     /locus_tag="SCK06_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG1151205.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6774..8111)
                     /locus_tag="SCK06_001189"
     CDS             complement(6774..8111)
                     /locus_tag="SCK06_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1151206.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8114..9217)
                     /locus_tag="SCK06_001190"
     CDS             complement(8114..9217)
                     /locus_tag="SCK06_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1151207.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9291..10799)
                     /locus_tag="SCK06_001191"
     CDS             complement(9291..10799)
                     /locus_tag="SCK06_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1151208.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10765..11457)
                     /locus_tag="SCK06_001192"
     CDS             complement(10765..11457)
                     /locus_tag="SCK06_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1151209.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11479..12453)
                     /locus_tag="SCK06_001193"
     CDS             complement(11479..12453)
                     /locus_tag="SCK06_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1151210.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12753..13625
                     /locus_tag="SCK06_001194"
     CDS             12753..13625
                     /locus_tag="SCK06_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1151211.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14207..16405)
                     /locus_tag="SCK06_001195"
     CDS             complement(14207..16405)
                     /locus_tag="SCK06_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1151212.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16637..17629
                     /locus_tag="SCK06_001196"
     CDS             16637..17629
                     /locus_tag="SCK06_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1151213.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17689..19110
                     /locus_tag="SCK06_001197"
     CDS             17689..19110
                     /locus_tag="SCK06_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG1151214.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19103..20602
                     /locus_tag="SCK06_001198"
     CDS             19103..20602
                     /locus_tag="SCK06_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG1151215.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20641..22035
                     /gene="uxaC"
                     /locus_tag="SCK06_001199"
     CDS             20641..22035
                     /gene="uxaC"
                     /locus_tag="SCK06_001199"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1151216.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6700 a         4510 c         3837 g         7077 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaattga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttactgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg ctaaaaaagc aagtgaaata aacaacacaa cggcaaacag
     4801 accagtcatg tcctgtaata atccaaatgt attttctaaa gacaaagttg aatgatctac
     4861 agcaagcgac tgataacttt ttttaaactc tgacgcagac atattactta aagtaactgc
     4921 taacccgcca aagaaaatca aagatagact gccaccgacc attcctagca atagttccca
     4981 ttttcttttc atttctgaac cccctgcttt tctaaatgta tccattcagg tcttttgatc
     5041 gatgtttcat aattgttatt acgttcagag aatttgacag tctgttcatt tttcttgtca
     5101 tcccaattat cccaaccagt tgggtgaaca tgcccaccta tcttacacgc ttcaaataag
     5161 acatttgcat acggacgcca cggtcttcct aaatagacgt ttctagtatc aggttctgca
     5221 gtcaaggtac agctttcaaa acgataccct gttttttgat ttttcggcgt agatgctgct
     5281 gtgatgtatc caggattttt actggctaag ctatggatat gacactgatg aaaaacggca
     5341 tctgccccac caaatatgaa atccaccgtt ccttcaatcc agcaatattc ataggtctga
     5401 cgacaatact caggattggc agaagccggt gtacaaaaag gagtaccatc tttttgcaaa
     5461 tcaggcaacg gacccgtgca taaggtatcc tgataaccag atagtcgaca gttttttaaa
     5521 gatacgcgat ctcctgaatt gaataaagca acagcctgtc ctaccttttc tcctggacct
     5581 gctgtatttt cgatgtgaag attttctaat acgacattcg atccttctaa gaaaaaagta
     5641 gcacttcgga aagtccctaa ctggctaccg tcaagatact cctgttttgc aaaacgatta
     5701 ccggagataa ctactgttcc tgctcccaca agtttaaaat tggataatct gctttctata
     5761 tattcatcat atcttccttc ttttactatc aagacctttg gatggctatc cggcaggcct
     5821 gccaaataat cgattccttc ttggatagtt tcaaaatccg tcggggtatc tatagcattc
     5881 tttccaatta tcagtttgtc tttcatcctc ataaatgctc ccttatgaat tgtttccaaa
     5941 tatttctcat ttcttgtgtt tccatatgac cgcctgttaa agaaaaagaa gaaaagtttt
     6001 ctggactacc cattgattga tactttttgt ccagcccttt tctcagcttc agtacgcctt
     6061 cgtccaagca catcgtatcg ttttttccaa ctaagctaag acgagggcgc ggagcgattt
     6121 tttcttggat tgctaacgtt gtataagctg tcaatagatt tggcacatag taataaaacc
     6181 catgatgatc taggcggcga tgtagcaaaa gtgtttcgat gtccacctgt gccgctagat
     6241 caatacaaac tttgacacga gaatccaatg ctgtcaccca ccagcttaat aagcctccca
     6301 tagaaagacc aatcgttgcg agccgttgag tatctgtgtc ttcccttgtc tccaaataat
     6361 cgatcaagct gatcacatca taaatacgca ttccccataa cgtttttcct gttaataaaa
     6421 attgtttgaa caactcgctt tcagaaatac ctccgcgttc ttcaaatccc caagcatcaa
     6481 tcgaccaaac agcatatccc attcctgtca gtacttctgc aaaagatgga gaagctaagt
     6541 aaggagcacc atggatcaat tcacttttcc cgactgaaaa atctccgccg tgagaatgca
     6601 ggtaaaggac cgtcggaaac ggtccttctt tttctggata agcatagagg gctggtactg
     6661 tttccaattc atttaagtac aattgatagt attcaagcat aaaaccgttt tgtttttctc
     6721 ggtataatcg ttttccacta ggggtatgtc tgctagatgg agcacctaaa aaatcattca
     6781 actgcataag caaagttttt acgaacaacc agttcatcca tattttctga ttgatttgat
     6841 tggaatttct caatactgat ttctttagta ttttctaata gaaatgctgg tccctcgtga
     6901 ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa agccaatata aaatccttgt
     6961 ctgctcattt cctctattcc tgtcatcata gcaggaacac cgggaactgc atcttgggcc
     7021 atcgagacat cgatattgtc aaatgtgatt tcttgtatga attgttccgc taatccataa
     7081 ataaaacctg cagaagcatg gacattcctt gccgtgatat tcgaaaaatg gatccttctg
     7141 aatgctgatg tccgttcatc tatcggataa gccttttttt cccatacata cggttctttc
     7201 cctcgcggac cacagaaata ataaagattc agtataaatg gacacatcac attatccatg
     7261 actatattgg atactcgaat atcttcaacg atcccgcctc gacctcgtct agatttcaat
     7321 cgtattcctc gatcagtttc ttggaagata caatttgaaa tggtaatatt acgaatacta
     7381 ccactcattt cacttcctaa tacgacaccg ccatgtccat ggaccatcgt gcaatttgtg
     7441 atcgtgatat tttcgcaagc gatcctttcg tacgtatctt cagtccctgc cttgattgca
     7501 atacagtcat ctcccacgtc aatatggcaa ttactaatac gcacattttt gcatgattct
     7561 ggatcaattc cgtctgtatt tggtgaatcc gctgggttca agatcgtcaa gttatcaaac
     7621 gttgcattac tgcaaagaat cgggttgatc gtccaacttg gtgactggat caatttaata
     7681 tctttcacag taattcgatg acagttatgg aaactcatca atttaggacg aggataagcg
     7741 agattatctg gttcatttct aaaagtatgc caccattttt ttccatttcc atctaatgta
     7801 ccgaaaccag tgacagagat gttctctaca ttttgagcat agatacagga agcataaact
     7861 tttcgatgga ctccttccca acgtgaatga acaacaggat aatcttttgg atcgtcagag
     7921 aatttcagaa ccgctcctgc gcttaaatga agttcgacat tgctttttaa aaataaagct
     7981 cctgttagaa attcacctgc tggaacaact acggtcccgc ctccgtcact tgctgcagca
     8041 tcaatggctt gttgtattgc ttcagtattc aattcatcaa ttgatgcgcc aaatgtcaaa
     8101 atatcataca tatttataat gcctccactt caatcaatgc ttgcaagaat gcaccaacgc
     8161 ctttttgatc attacaaata attggttcac tgatgtagta agcatatgaa ccatctcgct
     8221 gatctgctcc gcctaaccct gctacttgac agtttttatt caagttcagc cagccttctt
     8281 ttgtttccag aacaaattca tcaagtaatc cttgatgtga tttcttcact tgtggaagcc
     8341 attcttcttt gttcagcagt cccaaacgta ttcctttagc cattgcgtaa acaaacatac
     8401 tactgccgga agcttccaga taatttcctt tccgttttgt ttctgttgtt acttgatacc
     8461 atgtcccgct gttttcttct tgatatggaa gtaatgcagc cagtgtcttt tctagaattt
     8521 ctttcagcaa agaacaatct gtttcttcat gaagtaattc ataggtatct gctgcagcca
     8581 tcaaatacca gccaatcgat cggctccaaa aatgatggga tagacctgtt tttggatcag
     8641 cccatggttg gaccttcttt tcatcccatg catgaatcag caaatttgtt tttggatcga
     8701 atagatgttt ataactgatt tcaaactgtc tcaaaacatc ttggtaatct ttttcttcac
     8761 taaaattctg gataaattca gcataaaaag gagaacccat atataagcca tccaaccaaa
     8821 tttgataagg ataaatttct ttatgccaaa aagctccttc ggatgtgcgt ggatggtttg
     8881 ccaactgctg ggaaaggagt tctgctgctt ttttatattt ttcttcgcct gtttctttgt
     8941 acaataaaaa gaaaagcttt cctgtattta catggtcgat attgtattct tcaacgctgt
     9001 agccacggat cgttccatct tcttgcacga aatagtcgat attgtctttt atgaaatcaa
     9061 aataaatttt ttcttgggtt cgttcccaaa gtaattggaa ccctttaagt acgacgccat
     9121 agtcataaga ccattttcct ttataccact tttcttcata taagcgaggt gttctttcca
     9181 tgatactttt tgctaatttc tttgaccagt tttccattat ttgctctcct ttaaaaatta
     9241 aaataggatt tgggacaaat accaagaagt atttgtccca atcgtctata ttactgtgcc
     9301 gctgctttgt atgcttcctc gtactcttgt gtgattcgat cgcctccttg tgctttccag
     9361 tcttctacag cttttttgaa tccatcttcg tcaagctgtc ccatgatgta ttgcaccgta
     9421 gcatcaatga cgattttttc aatttctgat cctacttcat tgttcgtcgg tgaatccaat
     9481 ggcacagaag gatccattac agcaaattct tcattttctt tgatcaattg attagagaat
     9541 tctttttcag ggtctgtatc tttgaaagag aacgtcactt cactaggacg agagcttgat
     9601 aacggttgca cctcttgttg ccaaagatcc atatcgataa attcgataga accatcatca
     9661 ttcaattcat aatgcgttcc ttcgataccg ccagtcatca atttatacac atcttcttct
     9721 aaaagatcat caacaaattg aagtaatttc ttcaattgtg cttcgtcttt tacttgagat
     9781 ttgggaaatg ctagcaagcc gccaatacca tttccttctg accaaacatg ataaccgccg
     9841 ccttcaccat ctgagatctt gtttacaggc actaattcca tatcatcttg aataccttga
     9901 cccatcgtct tcagattctt gatatccacc attccagtgt agattccagt tcttccttgt
     9961 gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa agtcttgtgc caaatagcca
    10021 ttctcaaata gatctcttga aaaattcaaa gctttcatat actcttctgt atcaaattct
    10081 ggagtgaatt tgccattatc gtctacagcc cagccatttg gtgctccaaa ataagaagtg
    10141 aaaagtttaa agctggtata acgaatatca ctaggcgcac gatcgccaaa accaactgtg
    10201 tcgtccacac catttccatc aggatcgttt tctgtaaatt gtttagctac ttcataaagc
    10261 tcatccatgg tagttggaac atctagtcct agattatcca accagtcttt tcgaatgact
    10321 aagccagcac gagcgtagtc tttttgtatc ggtacgccgt acaaggcacc gtcgatactc
    10381 gcagcttcga ttctatcttc agaaatttga cttaaatttt tatagtcttt taaatattct
    10441 gatacatccc agaatacgcc tgattttaac gcattgcgga cagaagagtt tttcaacatc
    10501 gcaatttgaa gtgtaacgat atctcctagt tcatcagaag ccaatgcagt tgttaatctt
    10561 tcttcttttg aagcatcagg aatccagttg aatgtcagtt ccgtatcggt tttttcttcg
    10621 atcaagttca caatcgtgtc tgtaggcggt gaagcatgat gcaaaatatt taaccaagtg
    10681 attttagctt ttccatcttc tgtctctgaa gaactgttac ttgaactgct gccgcatgcg
    10741 gcaagtgcta gaattcctgc aaaactaaga cctaatttta cgagcgttgc ttttttcata
    10801 aattttctcc ttcactgttt taataatatt tttgttttct tctgtttttt ctgtatatga
    10861 gttcttccat tccaaatggc aaaatataga atttgctgtc cacgtgactg cggaaaagaa
    10921 gagactgcca ccgataaagg ttaaaacacc tggcattgtt tggatgatca tcagataaat
    10981 ccctaccata cttaatatca acactaatga gtaatgaaga tgtgagaatc cgaataaaaa
    11041 tgccattttc attttgaaaa aatatccttt ccaatcataa tgagccatga ttgggaaaat
    11101 atagagtgct gagagcacat atatgaacgc agcaatatac aaagcgatcc taagatacca
    11161 attgtgtatg aaagaaagat ccacaagtaa aacaatcccg atggcgccat atatccagct
    11221 catcagcaag gtttcacgat agttttcttt aaaatatgtg aagtacgttt tcgtcaaagg
    11281 aaacgtttct tttgagcgca gccattttct aatcactgca accatcgcat acgatgcagg
    11341 accgaaagta aaaactccca aacctgcgat gactaaaccg ccccacagaa aattcaaata
    11401 aaccaactgt aaaatacgat ttagccttcc gtttatttta tctaatgact gcagcatatc
    11461 tttccctctc tttctatctt agtaaacgcc gtcttctcct agctttttgg caatcttatt
    11521 agctaaaatt accataaaca atcctacgac ccctttaaac aatcccattg ctgtactgaa
    11581 acttaactgt ccattcttca atccagctgt ataaatatag gtgtcgaaaa tttctgcaac
    11641 gcttcgattc aatgaattca ataacaagta catatgctcg aaccctaagt ctaatgtctg
    11701 accgattttt agaatcagca aggtaatgat tactggacga attgccggca atgtaacatg
    11761 ccatgttttg cgtaagcgtc ctgctccatc catttctgca gcttcataca actgtgtatc
    11821 gactgcagtt atcgctgaaa ggtagatgat cgtagaccag cccaactctt tccagatcac
    11881 ttgtccgata tatactgtgc gtgtccattc aggggatgtt agaaaactga ttttttcaaa
    11941 tcccattgtt gcaaggaatt cattcaatac ccctccgtca acattcaaaa atacataagt
    12001 aatcgaaaca atgattaccc aagacatgaa gtgagggatg taaatgatcg tttgaatccc
    12061 gtttttcaat cgtttgttcg ttacttcatt taatagtaaa gacaaaatga ttggcatcgg
    12121 aaaaaacacg aagatattta atccgaacaa gatcaacgta tttcttaata atgtaaagaa
    12181 tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg acccaaggac ttcccattat
    12241 tcctaaaaat ggttgataat cttgaaaagc aataacgagt ccccccatag gaagatacct
    12301 aaaaatcaag aaataaagga ctcccggcag aatcatcaca tataaaaatt tattcgtttt
    12361 gattcctgca attatttttt ttcgtcttct gagcttttct tcttttttcg atagcgtcag
    12421 ttgaaatggt tcttgtttct ttacactttc cataagcccc tccttagttt tgtgaatgtt
    12481 tgctgcttat gacttcatga tagataacta tctgaattac gtatataatc atttttatcg
    12541 taagtaaaaa cgcttacata gcaatgattt ttgtgctata caaaacgata aagtgtattt
    12601 tttcacaaat aaactacgca tagcaaacct tctatttttt tctatctgac acttagcata
    12661 ggaaatgatt atatacgtaa tctttcaccg atgataaatt gaacatatag aaacagaaac
    12721 atttaattta ttttgaggag gagcctatct atatgaaaaa gaaagtctct ataggagaaa
    12781 gaagttttac acttttcaac aatacgtttc taattttgct agctttgatc tgtatcgttc
    12841 cttttttaaa tatcatcgct acatcttttg cttctacaca agaagtcgtc gcgaaaaagt
    12901 ttatcctttt tcctactacc ttttctttag acgcgtatcg ctacattctt tctacgccga
    12961 ccatttttag agcacttgct gtttcgattg gtgttacagg tgtcggaacg atcgtcagta
    13021 tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa gtatttgttt ggtcgaggtt
    13081 tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg ggggatgatt ccaacgttct
    13141 tagttgttcg ttctctaggg ctggtcaatt cttactgggc catgatcctg cctgtggctg
    13201 ttaatgcaat gaatatgatc attatgagaa actttttcca agccttgccg gatagtttgg
    13261 aagaatcagc aaaaatggat ggttgcacag attttggtgt atttttcaaa attatgctgc
    13321 cattagcttt accgtctatt gctactatct cgctttttta tgctgttact tactggaaca
    13381 cttatatgac tgctattttg tatatcaacg attcatctaa atggccgata cagattcttt
    13441 tacgccaaat cgtcattgta tcaagtggga tgcaagcaga aagtagtgcc gttgatgtga
    13501 ttccaccagc acaaacagtt aaaatggctg ttatcgttat tgcaacagtg cctatgctga
    13561 ttgtttatcc ttttgttcag aaatattttg tcaaaggagc actcgtaggt tctgtcaaag
    13621 ggtgattatg tactgaacgc aaaatggtag ttgtgtcaga agtggtcagc ttcaagaaat
    13681 aagaaggcat ttacgaaaat tgcttcttaa atttttgtga aatttcggct tatttttcga
    13741 agaatctact cctggaacac cgtgtatcag aggttgtgaa agagacgttc agccttgagc
    13801 gatgataaaa actctcacaa acattgcgac gagtaaccca caggagcaat aagaagcgat
    13861 tttgaaaaat agcttcccat atttcacaaa attatgcctt attgtcgata gcctgtctct
    13921 tgaacgccat ttattcgtaa aaaagaactg tgacaagact tttgtcacag ttctttttgc
    13981 gtgcttagcc ttgaaattta actatccata aatacaaata ctagggttat tctaacttct
    14041 tttattgctt gaatcagtcc acaagaaaaa cagcctcttg attaaacggc gtttatcttg
    14101 ttgattctgc ggcaataaaa tcagccagca gcaaaacatg aagtgctgtt ttttgctgtc
    14161 ctcttttatt gcttgaatca gatcacaaga aaaacagcct cttgattcac aaatattttt
    14221 ttcgatattc tcccggcgta atcgattcct tctttttaaa gaaacgaata aaattttgcg
    14281 gatttcgata ttgcaatctt tctgcaatct cttttacgga gaggttagtc tctttcaacc
    14341 atttttttgc tacctctaaa cgatgatttt gaacaaaatc accaaatttt tctcctgttt
    14401 cctttttaaa tacattactt aaatagttag ggttataatg caacctatca gcgatcacat
    14461 ctaaagacag atcacgatca tattctgtgt agacgatatg cataatattc tccgacaaat
    14521 ttttaaattc atgatcagtg attgcttgag agcattctgt gattggcaga actaattgtt
    14581 ctaaaacaag tttttctagt tctttgggat gataattgtt cagtgctttt acataaattt
    14641 gtttcatgtt atcaaagttt tttgaatcta ctcccaataa ctgtcctaat tgaacgagtt
    14701 cattgatcaa gcggaccgaa actacttccc gactcaaagg atttttattt tgtgcaaaca
    14761 atgcatcaat caattcatgt actaaattct tcacttcctg atttccagaa cgaatggctt
    14821 caaacaatcg attttgttct tctatcggat atctaataag tgttttttca tgagcagcag
    14881 gaacgatttc ctgataaaaa ataattgaac ttggaccagt atttacacga taatacaggg
    14941 cttcttttgc ccgatcaacc gattgcttgc tttcattcag cgtttcgaac cggttactga
    15001 tgcctatact gatagttaaa ttcaagtatt cttttacacg tttttgaatg agtcggcaat
    15061 actccatgac ttttttgctt gcttctgcat catctttatc cattcgataa atagtggctt
    15121 gcatttcttc attcaaaacg atcggcagca ttcgattttc ctttggcaca atttccgtga
    15181 tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc accgtgacta tcttctaaca
    15241 gatcgatttg aaccaaagct gtataatagc agtcattttg agaactataa ccaaattgct
    15301 gcaaccgctg atctagttct cgcctttcta ctcgatttct aaataaacta agtacaaata
    15361 gcgtttccag ctgtggcttt tgggtaacta agtgtgcact taacgcttct ttttctccta
    15421 tgatccgact gactgattga gcgaccaaat ctagttcttt cccactgaat ccttttgatt
    15481 taagattcag acgttcctgt atttgagaaa tcggacgggc aaatctgtcc gaaaataagt
    15541 aggaaaaaag accaatgagt cctaccagtg ttaaagacac tgcaactagc ccgattctca
    15601 gattgcggat cattgcgcca attgcactag agtcaacttc taatctgtaa atccaattat
    15661 tataatctga tttcagtact atatacgttt tatctttttc tcttatcact ttcagctgat
    15721 tgtaatctag atccacgtcc gttaatacag ttggttgctc tttatttttt actgtctgag
    15781 tagtaaaaag gttgccaact tcgttttcaa tagaaagtat gtcttctcca ctttcaccaa
    15841 caacttgttc tattgtatgt ttctctatcg tagcaattcc caatgcatat ttttcttttt
    15901 caaacattgg caaagaaatc accatttcga ttcctttgtt tttcttttgc caaaataaat
    15961 tatttttgtt atttatgtac ttctgttgat acttttcaac ttcctcttcg gtcagttgac
    16021 tcaacgatcc attgattact ccccatttac cagccaaact gactaaatca taattattat
    16081 catccataac aatcgtttct ataaaattta gttctttcct gatttcagaa taagtggagt
    16141 aatctgtata tgtcagcgga tgcttgaata tgtccgagaa atttttggtc gaaccatacg
    16201 tagaaaacga atattcaatc gttctaattt tccattcaac gttagtttta acttgcctga
    16261 gcaaattctt tttgtctgtt aaataactcg attcgatcga ttcataggta ttctggtaaa
    16321 tgaataaact aaatcctatg acaagtacgg ctcctaataa aaacatcggt aaaaatattt
    16381 tataaaatac tgtacttttc ttcattttct tcctctcctc cttttaaaat aaaaggcttt
    16441 catttttctg taaaattgat actcatctaa ttaaatgagc attccccgct ttcaagaata
    16501 gcataattaa aaataaaaat ccaagtaatt tttaacgtgt gcaatctttt catatcaaag
    16561 atctattgac ttatcgtagt tatcgtaata tgatgaatgc gttaacaaaa agtgaaaaag
    16621 taaggaaaat gatggaatgg taacgatcaa agatatcgct aaagcagctg gcgtttctca
    16681 tacgacagtt tctcgtgctt tgaataacag cagtttaata aaaaatgaaa caaaacaaaa
    16741 aatacgacaa ctagctaagg aaatgaatta ttcgccaaac tatagtgcaa aaggtttagt
    16801 gaatcaaaaa aaatatctaa tcggactatt tttctcaagt ttgcataaag gaacttcatc
    16861 aaatttccta gcagattcca ttgcaggcat ccattcagta ttagacacaa attacagttt
    16921 atctgttgaa agtatcgatg ttgttgattt aacagaaatc aacctgcaac gctacgatgg
    16981 actgatcgtt atgagtcaaa gtgatgatga tcagcctttc atcgactatc tcaaagaaaa
    17041 agaatttcct ttcgttgttg tcaatcgtca catcaatgat ccagcggtta tcaacgtcac
    17101 tgccgatgat gcggccggtg ttatgcaggc agtagattac ggtattggat gcggacatac
    17161 gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg acaaaagaac gggaaaaagg
    17221 ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat ctagcctatt ttttaacagg
    17281 agattacagc ttagaaagcg gcctccaaaa catggaatac ctattatcac tttctgacct
    17341 gcctactctg gttttttgcg gcaacgacga catagcgatt ggtgcattga gagctgtaca
    17401 tttagctggc ctccgtgttc cccagaacat ttctctgatc gggttcgatg attctccagt
    17461 cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag cctttgaaag aaatgtgtca
    17521 agaaggaaca aagcttttac tagatttgat cgaaggcagg acgatcgaac aatctaaaat
    17581 tgagctctcc accttgttga tccttcgtga gtcgatcaat tacttgtaaa ctacaagtaa
    17641 ttttatataa aaatgttaac gtgtgcataa cacagaaagg aagtttatat gaaaaaactg
    17701 acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag ttcttcagtt tggtgaaggc
    17761 aattttatgc gtggatttat tgattggcag attcagcagc tgaataacca acaattgttt
    17821 aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc ttggaaatat gatgaaccag
    17881 caagataatt tatatacggt cattttacaa ggactcagca atggcgaaat cgttgatact
    17941 tccgagatta tcaccgtagt agaacaaatg atcaatcctt atgatgaatg ggatgcttat
    18001 ctatctctag cagaaaatga cgacttggcc tatatcgtct ccaatacaac agaggcagga
    18061 atccaattcg tggagactga taatttaaca gatactcccc cacagagctt cccgggcaaa
    18121 ctgacagcct ttttataccg acgtttcaaa ttaaacaaag ctggctttac catcattcct
    18181 tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa tcattttacg ctacgcagaa
    18241 aaatgggatt tagaacctgc ttttgttgag tggttagaaa aagaaaatgt tttctgttgt
    18301 agtttagttg atcgcatcgt cccaggttat cctcgcgaag atgcaggtgt tttcaatcaa
    18361 cggcataact atgaagatca attgatggtc aaagcagaac cattcatgct ttgggtgatc
    18421 gaagggccta aagaattgga gaatcaacta ccattcaaac aggctggctt gaatgtcatc
    18481 atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc atttgcttaa tggaccgcat
    18541 acagcgatgg tccctcttgc tttgttggcg gaattggaaa ctgtagaaga agtaatgaaa
    18601 gatcctttat tctccgcata tgttgatcag ttattcaatc tagaattgat tcctatgctt
    18661 tctttaccta aagatgaatt agccatttat gctgaccaaa tcaaagaacg tttcttgaat
    18721 ccttttgccc atcacaaatt agaagcgatt tcattaaata gtgtttcaaa gttcagcacc
    18781 cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa atcaggtacc accgttaatc
    18841 actgtttcac tagctgcgct acttcttatg tatcgaggcg atcaagtaaa gccacatgat
    18901 gatgaaaaga cgataagcga atttactgat gcttggtctg ataatggaac agctattcct
    18961 cgattattgc aaaacgctgc gctttgggga gaagatttgt ctcaaatacc gaatgtgaca
    19021 gatacagttc aagaaatagc tgcaaagatc gatcaaaaag gtgtccggtc agtattacaa
    19081 gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa ccaacaatga aattaaatcg
    19141 acgagacagt gtggtcgtcg cattaacccc tattccaaaa caaacacaac tgacgattga
    19201 tcagcaaacg atcaccactc ttgaagatat cccgcaaggt cataagattg cattgggtga
    19261 tttgaaagct ggagacaacg tgatcaaata tggctatcca atcggtcatg tgacgacaga
    19321 cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa accaatcttt ctggtgaatt
    19381 ggattatcga tatgaaaaag atgtccatcc aagtcattac ctttttgaga accgaacatt
    19441 tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt aatgatttgt ttttagtacc
    19501 taccgttggg tgtgtgaatg gcattgctga actgatcgtc aaacagttca aggaaaaaca
    19561 tccagatctg ggacagtttg accatattac gattttaaag catccttatg gctgttcgca
    19621 gcttggaaaa gatcatcaaa atactcgaga aattttagcg gacgcagtaa atcatcccaa
    19681 tgccggagga gttttagtat ttggattagg ttgtgagaac aatacagttc ctgaattcaa
    19741 aaaaatttta ggtgaatatg atgaagaacg agtgaaattt ctagtggctc aagatgttta
    19801 cgatgagatc gaacaaggtg tggctctatt agaagagctt ttaacagcgg cggaaaatga
    19861 ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt ttgaaatgcg gcggttctga
    19921 tggtctatcg ggaataactg ctaatccttt acttggtgca ttttctgact acttgattgc
    19981 tcaaggaggc agcacgatct taacagaagt gccagaaatg tttggcgcag aacaagtatt
    20041 gatggcacga gcggaaaatg aagaagtttt tgactcaatc gttgacttga tcaatgactt
    20101 caaacagtat ttcttgtctt acggggaacc agtttacgaa aacccatctc ctggtaataa
    20161 agcaggtggg atcactacat tagaagataa gtctttaggc tgcacgcaaa aatcaggaag
    20221 ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc agaaaaccag ggcttagttt
    20281 attgcaagct cccgggaatg atcttgtagc tgcctctgct cttgcttcat ccgattgcca
    20341 gttggtcttg tttaccacag gaagagggac tccgtttggc tcatatgtcc caacggtcaa
    20401 ggtatctacg aatacaactc tttttgaacg caaaggtcat tggatggatt ttaatgcagg
    20461 cgtcttatta gaacaaccaa tggaagtaat cttagaatcg tttgttcaaa aaatcatcgc
    20521 tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac gttcgagaaa ttgccatttt
    20581 taaaaacgga gtaacacttt gattttcata aaacagtata aaaagaaaga aggaagattt
    20641 atgtttttat ctgatgattt tttattaaaa gacgattggg cgaaaaagct ttaccattcc
    20701 tacgcaaaaa acatgcccat tattgattat cactgtcact tagatccaaa agaaatttac
    20761 gaaaacaaca atttcagcaa tctgacagaa gcttggttag gaggcgatca ttacaaatgg
    20821 cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca caggaaatgc ttctgatttt
    20881 gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta tcggaaatcc gctttacagt
    20941 tggtcacatc ttgaattgaa gcgtttcttc ggtattgact tgttgataaa tgaagaaaat
    21001 gcagagaaaa tttggacaca agcaaatgaa attttggcag ctcctgattt tcgccgtcgg
    21061 gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa cagatgatcc cgcagacgac
    21121 ttgcactatc atcagttgct tgctcatgaa gaaccagatt tgaaggttct accttcattc
    21181 cgtccagata aagcactcaa tattgaaaaa gaaggattta atgcatgggt cacactactg
    21241 gaagaagcag ctgaccaaac gatcaccacc tatcaagaac tgatagaagt cttagggcag
    21301 cggatagaat acttccatca acatggatgt cgtgtttccg accacgcttt agacatctta
    21361 cgctataaag aagcggatga aacagtatta gaagagattt ttcaaaaagc acgagctaac
    21421 aaactattat cttcagatga aatcgatgct tatcttacag aaacactgat acatttgacc
    21481 cacttctatc attctcataa ttggacgatg cagctgcaca tccatgctta tcgtaactgt
    21541 aatacacaaa tgttggaaaa attagggccc gataccggct atgatggaat gaatgatctt
    21601 tctttgacta ttcctctgca aaaattattg aaccgtgcag aagaaacgga tcagttgcca
    21661 aagacgatcc tttattcatt gaatccgaat gactatcctg cgattttagc tttgatgggc
    21721 tgtttccaaa aagaacagaa tggaaaactg cagttaggtt ctggatggtg gtacaacgat
    21781 actcgagctg gtatgcgcga tcaattgaca caatttgctg atggtagtgc tttggggaat
    21841 tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct acactagaca tgaatatttc
    21901 cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc gaggagaagc acctgaagat
    21961 gaacaacttt taggaagtct agttcaagcg attagttata caaatgctaa aaattacttt
    22021 ggcttttttg cctaattgat taatagtaac agtaacgaat caaaaacagg atagtgaaat
    22081 aacggaatcc gtttatccac gttttagaga agaagacgga ccaa
//
LOCUS       DARBER010000032        21912 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARBER010000032 DARBER010000000
VERSION     DARBER010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.032"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCK06_001200"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCK06_001200"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG1151217.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="SCK06_001201"
     CDS             complement(1172..1867)
                     /locus_tag="SCK06_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG1151218.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="SCK06_001202"
     CDS             complement(1880..2596)
                     /locus_tag="SCK06_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG1151219.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="SCK06_001203"
     CDS             2926..3534
                     /locus_tag="SCK06_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG1151220.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="SCK06_001204"
     CDS             3859..4182
                     /locus_tag="SCK06_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG1151221.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="SCK06_001205"
     CDS             4364..5734
                     /locus_tag="SCK06_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG1151222.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="SCK06_001206"
     CDS             complement(5864..7165)
                     /locus_tag="SCK06_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1151223.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="SCK06_001207"
     CDS             complement(7519..9477)
                     /locus_tag="SCK06_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG1151224.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="SCK06_001208"
     CDS             complement(9654..9920)
                     /locus_tag="SCK06_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1151225.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCK06_001209"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCK06_001209"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG1151226.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCK06_001210"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCK06_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG1151227.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="SCK06_001211"
     CDS             13264..13827
                     /locus_tag="SCK06_001211"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1151228.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="SCK06_001212"
     CDS             complement(14080..14601)
                     /locus_tag="SCK06_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG1151229.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="SCK06_001213"
     CDS             complement(14604..15446)
                     /locus_tag="SCK06_001213"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG1151230.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="SCK06_001214"
     CDS             complement(15439..16164)
                     /locus_tag="SCK06_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG1151231.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="SCK06_001215"
     CDS             16340..16636
                     /locus_tag="SCK06_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151232.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="SCK06_001216"
     CDS             17204..18796
                     /locus_tag="SCK06_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1151233.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="SCK06_001217"
     CDS             18809..19549
                     /locus_tag="SCK06_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1151234.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="SCK06_001218"
     CDS             19717..21066
                     /locus_tag="SCK06_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1151235.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="SCK06_001219"
     CDS             complement(21194..21784)
                     /locus_tag="SCK06_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1151236.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DARBER010000033        21624 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARBER010000033 DARBER010000000
VERSION     DARBER010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.033"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(205..1104)
                     /locus_tag="SCK06_001220"
     CDS             complement(205..1104)
                     /locus_tag="SCK06_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG1151237.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="SCK06_001221"
     CDS             complement(1114..1470)
                     /locus_tag="SCK06_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1151238.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="SCK06_001222"
     CDS             1825..2121
                     /locus_tag="SCK06_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEG1151239.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2644..3597)
                     /locus_tag="SCK06_001223"
     CDS             complement(2644..3597)
                     /locus_tag="SCK06_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1151240.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3620..4375)
                     /locus_tag="SCK06_001224"
     CDS             complement(3620..4375)
                     /locus_tag="SCK06_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1151241.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4372..5331)
                     /locus_tag="SCK06_001225"
     CDS             complement(4372..5331)
                     /locus_tag="SCK06_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1151242.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5324..6274)
                     /locus_tag="SCK06_001226"
     CDS             complement(5324..6274)
                     /locus_tag="SCK06_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1151243.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6424..6876
                     /locus_tag="SCK06_001227"
     CDS             6424..6876
                     /locus_tag="SCK06_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG1151244.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(6965..9232)
                     /gene="gshAB"
                     /locus_tag="SCK06_001228"
     CDS             complement(6965..9232)
                     /gene="gshAB"
                     /locus_tag="SCK06_001228"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG1151245.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9419..10417)
                     /gene="ftsY"
                     /locus_tag="SCK06_001229"
     CDS             complement(9419..10417)
                     /gene="ftsY"
                     /locus_tag="SCK06_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG1151246.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10428..11237)
                     /locus_tag="SCK06_001230"
     CDS             complement(10428..11237)
                     /locus_tag="SCK06_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1151247.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11246..14827)
                     /gene="smc"
                     /locus_tag="SCK06_001231"
     CDS             complement(11246..14827)
                     /gene="smc"
                     /locus_tag="SCK06_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG1151248.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14847..15533)
                     /locus_tag="SCK06_001232"
     CDS             complement(14847..15533)
                     /locus_tag="SCK06_001232"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG1151249.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15881..17662)
                     /locus_tag="SCK06_001233"
     CDS             complement(15881..17662)
                     /locus_tag="SCK06_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1151250.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17688..18602)
                     /locus_tag="SCK06_001234"
     CDS             complement(17688..18602)
                     /locus_tag="SCK06_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151251.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18629..19591)
                     /locus_tag="SCK06_001235"
     CDS             complement(18629..19591)
                     /locus_tag="SCK06_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151252.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19591..20535)
                     /locus_tag="SCK06_001236"
     CDS             complement(19591..20535)
                     /locus_tag="SCK06_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151253.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20536..21540)
                     /locus_tag="SCK06_001237"
     CDS             complement(20536..21540)
                     /locus_tag="SCK06_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151254.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6041 a         4621 c         3818 g         7144 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtggac ttattgccga
     2281 gaagcagcag atggaacatc gtttaaagaa gagatcggta caaaataaaa atatctaaat
     2341 ttaagtttta tctaagtttc gctttgtacc tttgattcaa atcaaaggag gcgatcttga
     2401 tgctgaaaaa atggatcaca ctattttttg ccactcccca ttctagaatc aacccgtatg
     2461 atgtagataa tgaattcacg ttgaaaaaag atttctcaaa taaataaaaa ccatccgtaa
     2521 aatcatcaat acgattttac ggatggtttt tgataacttc tttgaggata ggctcttgct
     2581 atttaagaat gtaagaaaca ttattgttac atagtatgag agtttaatcc tctgttattt
     2641 atgctattta aatgcttgat tcacatcttc gatcatcagt ttcattgatt ctaaaccgcc
     2701 cccgcttaaa taccagacat ctggttcaag ggagatgatc tgctgatttt taccagcatt
     2761 cgtttgtgct accagttcat tggcagaaat atcatctttg ctgtcgtctc caccaatagc
     2821 ttttgttctg tctactacaa ataagatatc cggatttttt tccagcacat attcataaga
     2881 aacactttga ccgtgagtgg atgcttcgat ttgatcatct gcttgttcaa agccaaataa
     2941 atcatggatg aaaccgaatc tagaaccaga tccgtaggct gataattgcc cttcattcac
     3001 aagtgtgact agtgcttttt tgtcagtagc ttctgctttc tttttcgtct gatcgattgc
     3061 actagataat tcctctaatt gagttttagc ttcttcttgc ttaccaaaga tctcccctaa
     3121 agcagtgaca ttttgctgga aagattccca tggatttttc gcatctaaag aaaggaaaat
     3181 ggtcggagca atttgactta gatctttttg aaaatctctt tgtcggccag aaatgatgat
     3241 tagatctggc tgaagctgat taattttttc taagtctggt tcttttattc cacctgctga
     3301 ttctactttt ttataggatg aaagatactc aggaaggctg cttgtagctg ctccaactac
     3361 cttatctcca acaccaagag catccatcgt atctaaagaa ccattgtcga atacgacgac
     3421 tttttctgga ttatttggca cctcgatttg gtctccatta ctatctgtta tcgtcaatgt
     3481 agtttcagtt tgtgtgctgc ttttttttgt gtctgcctga ctggtgttgt tgctatttcc
     3541 acatgctgct aaaccaaatg atgctagaat caagatacct actactaatt ttttcatctc
     3601 ttctctccta atgttagttt tagaaataaa gacagaatct gcggccttca atctcacaaa
     3661 tccgtatgtc catttcgtat aattcatcca atacatcttt ttttatcatt tcttgggtcg
     3721 ttccttgcgt aaataactgt ccatctttca ttgcaacaat ttcgtctgca taacttgcag
     3781 caaagttgat atcatgaaga acaataacaa ttgttttccc gatttcttgt actaaatttt
     3841 ttaaaatctg catgatttga accgcaaaat tcatatctag attgttcaaa ggttcatcca
     3901 gtaaaatata atccgtgtct tgcgctagca ccattgcaat atacgcacgt tggagttgac
     3961 cgcctgaaag agtgtcaatc gattgatccg ctatttcttc taacccaagg tgttccagtg
     4021 attcctgaac gatttcctcg tcttttgtcg tcaattttcc tttcgtataa ggaaatcgtc
     4081 caaattctac taattctttc acggtgattt ggacatttat ttcattcgat tgtcgtagga
     4141 tcgataattt tttggctaat tcattggatt tccaagcctt tacttcgtta tggtctaaat
     4201 agatttcacc tgtatctttt ggtatcaaac gactgatcat cgacaacacc gttgactttc
     4261 cagcaccatt tggaccgata aaagcagtca atttcttttc aggaatcgtt aactgtacat
     4321 ctttaaccac tgattttcca tggtacagtt tattgacctt ttgtaaatca atcataggat
     4381 actcttcctt tcgcgaatca attttgtgac aaaataaatt cctccgacaa attcaacgac
     4441 gacactcact gttgtattcc agccgaaaat ctgctctact gcgagctgtc ctcctgtcag
     4501 aaacagcata gctaccagaa agcttccgat aaatagctga ccatgacgat aagtagaaaa
     4561 gacacgataa ctgatatttg cgacaataaa ccctaaaaaa gtaactggac ccacaagagc
     4621 cgttgatgtc cctgtcaaaa gactgacaca acataaaatc aaaaattcaa aacgatctgt
     4681 gtctatcccg aggtttactg cttgttcatt tccgagatgg aaaacgtcta gcacatgtcg
     4741 atatcgaaat aacaggtacc agcagccgat aatgatcaaa gaagcaggca aaagatactg
     4801 actctggata ttgccaaagc ttgcaaataa tctgccttga agcaaatcat attcattcgg
     4861 atccatgatg acttgtaaaa aggtactgac actaccaaaa agcgtaccta aaatcatccc
     4921 tgacatcagt aataaaaaaa gattcgctct catttttttc aaaagaaaga aaaataacaa
     4981 tagactcagc ccgaccatca gcccaacgtt caataagaaa aaaactgtac ctgtctgctg
     5041 aagcatcgtc actcctccga taaagaagaa aaggaccgtt tggacaagga catataacga
     5101 ctcaagtccc aatactgaag gagtcaaaaa ctggttatgc gtaattgtct gaaagctgat
     5161 tgtggcactg ctgacagcta gcgagacaag gataaatgca aaaattttct tactcctcaa
     5221 tgcacatgca aaatgccagt ttccatatgt atggatcgtc aagtacattc ctgtaacaaa
     5281 taaaacaaga acaagcatac caataaaaat agatggcttt tttttaagca tatcgtcgtc
     5341 cccttatcag aagataaagg aagatagccc caccgatgat tccaacgatc acactgactg
     5401 gtatttcata aggaaaaatc aacacgcgag cgacaagatc gcaaaatact aagaacaaac
     5461 tacctgctaa agcagttacg ccaattgttt tgtgcacttg atctccatat attctagaga
     5521 caagattcgg aatgatcact ccgataaaag gaatgcttcc taccatgatc aacatgactg
     5581 cactagccaa tgatactaag ccaatgccta aaaactgcat cttctggtaa tctattccca
     5641 gatttactgc aaacgattcg cccagaccaa tgatcgtaaa tcggtaagca aatagccaaa
     5701 ccaagagcaa caatggaaca acagcataca ataactcata atttcctcgt atcacagtcg
     5761 caaaattccc ttgaagccat gaagtcatat tttgtaccaa ctggaattga taagcaaaaa
     5821 aagtagcaac tgaaccaatg atattcccta acatgactcc aatcaacgga agcatcaatt
     5881 gatttttctg gaggaacaat cgcgtaaggg ataaaaaaag caatgttcct gcaaaagcaa
     5941 agcaaaatgc tgtcaaggat cggacaatca aggatgcacc aggtaggaaa atcatagcca
     6001 ctaaaatacc taaacgcgca ctatccatcg tgcccgcagt cgttggtgaa acaaatttat
     6061 tttgggtcaa atgttgcatg atcaacccac aaatactact ggtcgctccc acaaggatca
     6121 aacaaatcgt tcgtggcaaa cgagtcacta gcaagatttg taagctttgc ccatctaact
     6181 ggagcagttc tgacaattgt acattcgata ctccgatgaa caatgaaata ctacttaaaa
     6241 taactaataa tgctactagt cgtccgtagc gcatccaatc cacctttcta attgagaatg
     6301 attttcattc ttattttaag tgaatcgttg aacaaaagca accgcttttt catatgtttt
     6361 aggtattatt gggaatatgc tataatcgta agaaagacaa agagaagaag gagggtattt
     6421 tttatggcac aagatgatgt aaaaagccat ttggataagg gactttatgg tacaccttta
     6481 gtgaaccctg aagaacagca taaatattta gggacattca gggaacgctg ctatcttagt
     6541 atgactgttg ctgaaatgat ccaaccaaaa aacaaagaaa actttttaaa agaactcaaa
     6601 aaacatccag atgcttccat actcttgaat ggggctctac ccctttctgt ccaaaaccaa
     6661 tatatccagc tagcaacgaa aaacaatagc cgtttcactg tagtcaacga ttacgtcact
     6721 gcaaatcctg aggaattcgg attgctctta acagcaaaag aagcagtaaa tgaacctgtg
     6781 attgatatcg aaaaaaaata cccagaagaa aactcttcgc cagctgaaaa agaaaaacca
     6841 aaaaagaaaa aaagcttctg ggatcgttta ttttaaaacg aaaaaacaca taaattttta
     6901 catgcaaaaa aagcttttga tcagattgac atcaaatagg acaatctgtc aaaagctttt
     6961 tcttttaaaa tacttcagga tataacaatt tcaatacatt catcgtcaat cttctgcctt
     7021 ttcctgcaaa aggataaaca tgcatatgca tggctggatt gaaatttgct tcgatgatcc
     7081 catatgcttt tttatcagtt gtaggatcga tttctttatc tggtataatc aaatcgatac
     7141 cactaatctt agctcctagt gcttctaccg cagaaacggc gatttttttg taagcttcag
     7201 aaaattcatc agtcatatcg atcgaatcac cacctgtact gatatttgag ttctccctta
     7261 gataaacaat ttgattggct tggggaactg attcgatcgt caacccttgt tctttcaaca
     7321 tcaattgttc taattcacct aattggatca attctaaagg tgcacgatga ttcgttccac
     7381 gcaaagggtc actatttttt tcttcaacaa gttctttcac tgttcggata ctgtcaccaa
     7441 taacattagc agggactcgc aacatgatgg cttggacttc tccgtcgatc acaaagaaac
     7501 gatattccgt tcccggcata aattcttcta ctaaaaccga actgtcttca cgaaaagcaa
     7561 tggctaaacc tgcttgataa tcttccagac tagctccttc cttgaaaatc gtgataccta
     7621 acccgtaatt ggttgattta ggtttgatca caaatgcctg ctcggcaaat ctcggataag
     7681 cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc tggtacccgg aacccagctt
     7741 cttttaagac ttttttcgtt actgttttgt tttccatgat aagtgggacg atatagctgt
     7801 ccttacttgt catgtttgca tttttcacat attctacatg gtcttggaat tgcagacgca
     7861 aaaattgatc cgactcatct aatactttga cttggacacc tttttggatg gcatcgaaca
     7921 taaatatctg agtagaaagt tccatttcgc gaaaaccggc caactgatag ggacgttcat
     7981 ggctttcttt ataatattga tgtcccaatg ccacagcaac ttcttgctga ctcgatagct
     8041 gtgcttcttg atagagtttc gctgctaacg tttgttcagg atgatccatt tgagtcaatg
     8101 cttcctctac taaataaaga gaatccaacc aatccaattc aaccagcatt tgcttcattc
     8161 cttcaagaat ttctctacct tcttgatgaa attctgtttg atctgatggt tgttctagtg
     8221 ctactttgtt atttcgagtc gtaccttctg ctacccattg gtcacagtct tctttttctt
     8281 ctgtccataa catgtacatc aggaataatt gtaagaaccg aacttgctct ggattgatac
     8341 ccaatcttgc ataaggattc aaatcgatat tccgcaattc aatataacga acacctgttg
     8401 ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag cggtgtataa aattctttct
     8461 cctccgaaag tttcccttct tcgatcattc gctcgatatc tgctaaatac tgtttcatag
     8521 aagcatagga aactttcaca tttggatgat tcgtatagcc cagcgcactg ttgcgaatac
     8581 tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta gttcttctca ctcattggtg
     8641 aagcaccgaa taaataagtg atcatccagc gatagcgcat aaagtttctc gctgttttca
     8701 aataaagttc tgttttgaat tcggaaaaat cttgaaattc ttcctgatgg ctgaacaatg
     8761 ttcttagcaa ctcatcgcca aattcaaaat tgaaatgaat accgcttacc atctgttttc
     8821 gcttcccgta ttcttttgct aaataacgtc gatacaagac atcttcgaaa ttttttaatt
     8881 ttgcaataat gatctcttcg tctttttccg gtaaggcagg tggcatactt aatggccaga
     8941 tcatctcttc tttcggtata gaacgagcag tcacatcata aacagcagcg agatactgaa
     9001 ataattcggg aatagaatcg gtaacagggg tgatcatctc tgtttgtgtt tcgctaaaat
     9061 ctgtttggat ataggggtga taggatcgat cgccaaagat tgctggatga tcggtttttg
     9121 ctagatttcc tgcaagatca acccgctgtc cttcccgttc tataccaaat cgagcttggt
     9181 cgataaaagg gcgcgcattg acatgcaata ataattgctt aaaattcatc attaagtgac
     9241 ctccaattca tatgaatcta attataatca aaaagtctaa gaaaaagaac gaaattgttt
     9301 gagaaccgct tacaaataag tataaaaatg aaggcatcaa aaacacagct atgttaaaaa
     9361 caatcattca aacaaggagc tgcaactttt gtcacagctc ctgcttttta gacatgggtt
     9421 attcttcttt tagtaatcct ttgaacaatc caacgacaaa atcatttgga tcaaacggct
     9481 ccaagtcatc gattccttca ccaagaccga ccaatttcac tggtaaatgc aattcattac
     9541 ggatagcaag tacgataccg ccctttgctg taccatctaa ttttgtcaaa acaagaccgg
     9601 tcacatctgt tgtttctttg aattgttttg cttgaaccat cgcgttttga ccagtggttg
     9661 catctaatac aagaagtgtt tcttggggtg cttctggtaa ttcccgctgg attactcgtt
     9721 ttattttttc taattcgttc atcaagttca ctttattttg cagtcggcct gcagtatcaa
     9781 ctagtaaaac atctgcctgc tctgtctttg ctctttccaa agcatcgaat acgacagctg
     9841 ctggatcgcc tcctgcattg ccacgaacaa cttcaacacc agcgcgttcg ccccagacaa
     9901 ctaactggtc gattgctcca gcgcggaagg tatctgctgc tgccatcaat acttttttac
     9961 cttcgttttt gaactgatgg gcaagtttac caatacttgt tgttttccct acaccattaa
    10021 caccgacaaa caaaataact gttagtccgt cttcttggat atttagttca tttacttcat
    10081 tgattccttc cgcttcatag agatccacaa gtttctcgat gatcgcattt tggacttctg
    10141 ccgatttttt cgcatttctg agtttcactt cttgacgtaa tgcttcggta attcgtatcg
    10201 ctgtttcaaa acctacatct gcaccgataa gtgtttcttc taattcttca aaaaagtctt
    10261 catcgactgt tcgaaaattg gcaaacaact cattcatccg ttcaccaaat gttttacgcg
    10321 tcttttctaa acccttgtcg tacttctcct gtacttcaac ttcttctgtt ttctcttcgc
    10381 cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg actcccccta ctctaataca
    10441 aatttttgga tcactttggc tacgccattt tcttcattgc tggctgtcac gaaatctgct
    10501 gcttctttga cgaaaggcac agcattctcc atcgctacgc ctagtccagc atattggatc
    10561 atcggtaaat cattttcctc atcaccgatc gccatgattt cttcttgttt caaaccaaga
    10621 tcttttgcca ataaagaaat accataagct tttgtaacac cttttggcat aaattccaaa
    10681 agcatacttc ttgttttgat gatctcatac atttcatgga attctgctgg tatttctttg
    10741 attttaggat ctaattcttc ttggtcataa gcgattactg ccttattaag cacaaattca
    10801 tctgtaatat cgcttatatc cgctggttgg aattgcagca gcttattcaa cacattgtac
    10861 aaggattgct tccttggggc actaggcagc tgtaaaacca cattgtcaga caaaacatct
    10921 agtggcagat ccaattgctg agccagtttg tataagcgtt ggatatctga tacttccatc
    10981 actttctttt ccatgatttc accagtatca tttttttgta ccaaaccgcc gttgaaagtg
    11041 atgctgtagt caccttcatc taccagtcct aattcttgca gataatgcgc catcgcagct
    11101 aaggggcgtc ctgtacatag aacgatcttg accccttgct gttttgcttg tgctaatgct
    11161 tgtttattct catctgaaat cagcttttgt tcatttaata atgtaccgtc caaatcgatt
    11221 gctactaatt tgatcatttt cgttcctaca ctttcgtttc gttgattttc gcctcttcat
    11281 tgatatcttc catccgtaca gaaacgattt ttgatacacc ggattcctgc atcgtcactc
    11341 cgtacagtac atctgctgcg accatcgttc ctttgcggtg tgtgactacg ataaattgtg
    11401 tgtcatcttg gaaatcgctc aagtaacgac caaaacgttt cacgttggct tcatctaacg
    11461 ctgcttctac ctcatctaag atacaaaacg gaacaggaca gacacggata atggaaaaga
    11521 gcaacgcgat ggctgtaagt gctcgttctc ctccagaaag caaacttaga ctttgcagtt
    11581 ttttgccagg aggttgtact tcaatctcaa tacctgtttt cagcaaatca gttggttccg
    11641 tcagtactaa ttcagcttta ccgccgccaa acatatttgg aaagacaatt ttgaattctt
    11701 gacggatagc ttcaaatact tctttgaatc ttgtccgcac ttcatcatcc atctcatcca
    11761 tcgtttcaaa caattggttt ttcgctgcta acagatcatc tcgctgtgtt gctaagaatg
    11821 tatgtctttc actgacttgt tcgtattgtt cgattgaatt taagtttact ggtcccagtt
    11881 tttcaatctg ttcttttaag gatgaaacct ttgttctact gtcttcgata tcggttgttt
    11941 cttgatagtc ttgggaagct ttctcaaatg tcagctgata ttctgactgc agataggtca
    12001 aatgattatc taaaatcatt tcagctcggt ctttttggac ttctaatttg gattgttccg
    12061 acagtcgtgc tttttgctca cgattacgct ctgccaagac tgcttcaagc ttatcgatct
    12121 ccttttgcct tctatctcgc tgctctttgg cttttgccaa ctcggctttt aatgtttctc
    12181 gctgagcaga taattcgtta atttgttttt ccaaactttc ttctgtgact tcatgatcag
    12241 aaaagtctgc tgtcaatgtc gccaactgct tttctatcgc ttcttgacgt tcggttgcct
    12301 ctgctttttg gactcgggca ccgcggagct ggatctgcag atgattaaac tgctctttca
    12361 agacagcaag atctgcttgt tcttgtgctt tttgactttg tacttgtgct cttctggctt
    12421 ccatctggtc gctttcttga cttaacgatt tgatttcttc gtcgattttt tgtcgttgcg
    12481 attcaagatc tgtttgtttg tctttcaatt cgttcgtttg cttttcgtaa tcttcgataa
    12541 actgctgcag ttcacgtgtc tcaaaagttg agatttgctt ttctttttca aaacgttcaa
    12601 gttcatttat gatgttttgt agctgatttg tcgcttcttg ttcttcaaaa cgtaattgtt
    12661 cgcctcgtgt acgcaaaact tcttgttctt cggctaaacg agctgtttct tgttgcagct
    12721 cctgaacttt tttttcttgt gcttggagtt gtttatctgc ttcttcaaat tcactagtca
    12781 actgtttcaa ttcttgattt tgtacaaaca aactgccttg gtttcctcgt ttattcgctc
    12841 cacctgtcat ggatcctccg gcattcatca catcaccttc taaggaaacc actcgatatt
    12901 gatagcggat cgtttgcgcg attgcattgg cactagttaa gtctttggct aaaaggatcg
    12961 ttcctagcag gttttgaacc acagtttgga tttgatcggg ataactgact tgttcactgg
    13021 caatccctat aaacccttct acagctgctg cttgtgtcaa tatatgagcc ggtaattgtc
    13081 ttggtttgat tgtcgtcagt ggaaggaatg ttgctcttcc tcctctttgc tgtttcaaat
    13141 acgtgatggc ttgccgagca tctttttcat tttcaacgat gacatgctgg gctgccccac
    13201 ctaaagctgt ttcgatagct aacgtgaaat ctgcaggaac atcgatcagt tcagccactg
    13261 ctccgacaat acccgaaagc tgctgtttat gctgcaagac caatcgaacc ccttggtaaa
    13321 aaccaaaata gttttcttgg atttcttgaa ggcttttctt acgagcacgt agctgctgga
    13381 cttgattcat taattgatac attttaggct gttcttttgc taatgcttct tggaactttt
    13441 tcccgttttg ttggatcatt tctaacttct cttttgtttc agttaaagat gattttagct
    13501 gcgcctgctt ttgtgtcaat tcttcttttt gtaagatcaa tcggtctact gatgcttcta
    13561 cttccgattg cttggctaac gtctgcttgc tttttgctgt ttcttgtata tattgacgtt
    13621 ctagatactt cagttcgttt cctacagctg cttgttcttg catcaaatca acatattgat
    13681 ctcttaattc tgccaataat tctttcgttg atttttggta tttttctaat tcttgctgtg
    13741 tttttatgag atttgcttca gctttttgga tctctgtttc tttttctgct gctgctttca
    13801 tcaagctttc ttgaagcttt tcaaaatgtt taaccttttt ttgtgcttct gctaatgaag
    13861 tttgatattc ttgcgagctt ttttgtgtat gctttgtccg ctcctgcaaa acatcttttt
    13921 gtccttctgt ctgcttcagt ttttccgaaa gatctaataa tacttgctga tttttttcga
    13981 tcagacgatc ggcttgcgca ttttctttac gctgcttcgc caaaatggat tcttgttctt
    14041 ggatcgactc agatagtttg cctaattcta gattgaattt cgcaagctgt gcttgtttgt
    14101 tgtcccaatc tttttttgct gtcttgattt cagcaaccat caaagagaca tcggtttgag
    14161 tcagtgtttc ttttagccgt aaaaactcct ttgctgcctc actctgggcg gccaaaggag
    14221 ttaactgttc ttccaattcg tggatgatgt cttggacgcg gctgaggttg tcttctgttt
    14281 caaaaagctt ctgttctgct ttctttttcc gctgcttgta tttcaagaca ccagctgctt
    14341 cttcaaagat cccacgacgg tcttccggtt tactactgaa aatcgcttct acttttcctt
    14401 gagaaatgat tgagaacgat tcttttccta atccagaatc taagaacaat tcttggatat
    14461 cttttaatct gcagctttgt ttattgatga aaaactcgct ttccccagtc cgtcgataac
    14521 ggcgggttac actgatctca ttgtactcca atggcagata atgatcacta ttgtctaaaa
    14581 tcactgtgac ttctgcaata ttcagctgct tacgggtatc ggaaccggca aagatgatat
    14641 ccggcatttt tccgcctcgc agacttttag cagattgttc tcctaatacc cagcggatag
    14701 cctctgtaat attgcttttt ccgctgccat ttggtccaac cactgctgtg acgctatttt
    14761 caaaatcaat gactgtcttg tctgcaaatg atttaaaacc ggctatttca attcgtttca
    14821 aatacaaagc tttccctcac ttacttttac agtgctgcca atgcattttc cgccgcagct
    14881 tgttctgcta attttttcga tttcccttgt ccagtgccaa tcaattgatc atctacataa
    14941 acttcgatcc aaaatactcg ttcatgagct ggaccttctt cattgatcaa tcggtattcg
    15001 attgatacat cacctgattt ttgcaagact tcttgtagtt ttgttttatg atccatctca
    15061 tgtgaaaaag cacctgcttt gactttagga aaaatcactt tttcgatgaa tgcatgggtc
    15121 gtgtcgaatc cctggtcaag atataacgcg cctaaaaaag cttcgaatag gtcgcaaagc
    15181 aatgctggtc gcgtacgtcc tcctgaattc tcttctccct tgcccaacaa aacatattga
    15241 tcaaagtgac attctttggc aaatttcgac aagctgtctt cgcgaacgat cgcagctcgt
    15301 gtttttgtca attttccttc tggcaaatga ggatataaac ggaataaata ctgagaaacc
    15361 atcagttcta aaacggcatc tcctaaaaat tcaagtcgct cattatccga taattgcaga
    15421 ttgcgatgct cattcacata ggatgaatgg gtaaaagctt gttcaagcaa atttagatcg
    15481 tgaaaaacga tgttatagtt ttcttttagt tcttttgtca gctggttgtc cattttccac
    15541 attcctttta tggatatttt ttcgatactt ccttattata ctggtttttt gtcaaatttg
    15601 aaatggttcg gtcaaaaggt aagtaaagct ttaataatcc tagtgattga cgagaagttg
    15661 tgaaaaattc gtttagtttc gagtatgaag aaaaaatttt gtgaaaatgg ctttccatat
    15721 ttttgcataa atttgactta ataccgagaa actggacttt gaacaccgtt tattggagaa
    15781 gtcatgaaat cagcgccttg acctgaatat tagagaaata aacagaaaaa tatcaaaaaa
    15841 ccgaaaaagg acatcgccct ttttcggtca tttttagtga ttatgaacgt gaatctgctg
    15901 taacagaaac atttgcccat gggtttggta catcataaga ccaatcccag tctttgacac
    15961 ggtcgttgac tggcaatact tcattacgga acaacgttgg aatcgcaaat gcttcttcaa
    16021 atgcatattg ttgccatttt tgatatgctt ctttttggta ctcttcatca aatgctttgc
    16081 ttgaactgat atcgttgatc aattttgtat tttcttctga ttcataacga gtgtagttga
    16141 acgcagcatt tggtccccat agtccacttg gatttggatc agttcctgta ttccaagcag
    16201 cttggtaaac atcaatttca ggatcatcgt tttctaattt atcatagaaa gaattgaatt
    16261 cgatcagacg acctgttgtg tattggacat ccaaaccaat ttctttccat tgttggatat
    16321 agtaatctgc caatggttgc gctgtttccc caccggacat cgaagcaaag ttgattgtta
    16381 gtttatttcc atctttgtct tctcttaaac catcgtcatc cacatctttg tagccagcat
    16441 catctaatag ttttttcgct ttgtctagat cttgtgtgaa tcctttcatc tcatcatcat
    16501 ggtatgactg gaatactgga ggaatcaatg aagtcgcatt gcttctcaaa cctgcataga
    16561 aacgttcacc tactgcatta ttatctaatg catagcccat tgcttgacgt aatgatttat
    16621 cagccatttt tgaatctgga ttgtattcca cactctttgt atctgcattc catttaccta
    16681 acttgaatcc tagataagta taagaagttt gttcacggcc taagtttgta tagccagcaa
    16741 tttctttata ggtatcgtaa gtatctgttg gcatttggaa aatcatatca tataatttag
    16801 agttcaatgc ttcagttgca cttgccatcg gtacagattt catcgtgatc tttttcaatt
    16861 taggcgtacc attccagtag tgctcgtttg gcaagtattc tactgattct cctgcaacga
    16921 tattactcat gtaataaggg ccaaaagtca ctggattttt acgtacttga tcgcttgatt
    16981 ccatatcttt gattgctaca ccttctaacg tatgttttgg tgctgcatat gaccagatac
    17041 caccacccga ttgaagcatg cttgggttta cttctttata ttcgatttca actgttttgt
    17101 cgtctatttt tttgattccg gagattgtgt cggcacttcc atcatgatat tcttccatac
    17161 ctacgatgtt acggaaagtg tcgtcataac ggataccagt gtaatcttta ttcccaatga
    17221 tttcgtaagg gaaaatcaca tcgtccgccg ttacatcttc accatctgac catttgacgt
    17281 tgtcttttac tttgattgtc gcttttttcg tatcttcatc tagatcaagc gttgcagctc
    17341 cgtcgtctgt gattctgaaa tcgtcatcgt ttgcaaatag cggttcatga gaaggagcca
    17401 taaaagctgc atcatatgaa tcttggtaaa actcccactg gaacaatcct ttaaactgac
    17461 tgtccgttgc gaccgcagct tctaattcgc cgtcttcgat tgcatcatca ttattttttg
    17521 ttgcaattgg aaaagttaat tcctctttgt tattgccgtt gcctccagag ttgtttccag
    17581 aggtatctgt acctccgccc ccgcatgctg caagaacgac agcagacatc agcgctgcaa
    17641 atccaaaaat cgttttcttc attcctattt cccccttcgc ttctatacta gcctaaccgt
    17701 tggcgcgcat ctgcagaacg cttgaatgct tgtccaacat aattgatact caacatcaaa
    17761 actaaaataa ggattgatgc gggtaaccag atccattgcc gattcactaa gacttctccg
    17821 ttacttgcat aaccgatcaa tgtacctaaa ctaggtacat ttgccggcaa gccgaaacct
    17881 aagaaagtca aagttgtctc gataccgatg tttgccgcaa agttcatcgt caaattagta
    17941 atgattaatg aacttagatt cggcatgatt tcgcggaaca tgattttaaa atcattggtt
    18001 cccattgttt ttgaggcact gacataatct cttcttgctt cagataatgt cttgcttcgg
    18061 aataatcggg ctttagctac ccaataaaat gcactcatga tgaatataaa ggaccagaca
    18121 ttgtatctgg aaataatcga tacgaagacg ataatgatca gcatgatcgg tagaatcatg
    18181 ataaaatcaa cgatccgcat caagatgttg tcaatgatcc ctccgtagta gccagagatg
    18241 atccctaatc caaccccgat gatcgaagta aggatcgtga tcgcaaatcc gatcgtaaca
    18301 gagtttctag cacctatgat caattgtcct aatacatcac gtccgccttc atctgctccc
    18361 agaatgaact tttgtccttg tgtcgaaact gttcctggtg cagcgtattt gtcgaagata
    18421 ctaacataca tcactttgtc ttgatcggtc agcatcgcac cgataaagat cactaataat
    18481 aaaaccacta gcaaacctaa tgaaaaaata gcaattttat cttttttaaa ttctcgagca
    18541 atcattcgta tccccatagg aggaatactt tcttgaactg ctgtattgtt tttttcactc
    18601 atcgatccat tctcctttct gattggtttt attgaatccg aatacgcggg tcaacgatac
    18661 tcatgatgat atctgaaagt aatgtcccca ctagcgtcgc gattcctaaa atcaatacta
    18721 aggatgtgat cacggtatag tcccgttggc cgatggaatc aataaatagt tttccgactc
    18781 ccggataacc aaaaatcttt tcgataacta cagaaccagc aatcaacgaa gtgatttcat
    18841 accccatttg agaagccatc ggcaacgctg catttcggaa aatatgtcga ctgaatactt
    18901 tattcgtcgg tacccctttg gaacgggctg ttctgacaaa atcgagcgat ttcgaatcga
    18961 ttacttcact tcgcagatat tggattgtga cggcagtcgc caaaaatgct tgggtaattg
    19021 atggtaacag aatatatttc aaacggtcta tccattgagt gataaatcca ccttgcaaac
    19081 ctagtgtttg tgatccactt gttgggaacc agcctaaacg ataaccaaaa acaaatagca
    19141 tgatcaagcc gaagataaat aaaggaacag caaaactaaa gaagttgtag attacgacaa
    19201 tcttatccaa gattgaattt tgataccggc cagcaagcat ccccagaggc acagcaatca
    19261 aataagtaag gacaactgtc aagatcgata gatataaagt gttgatgatc cgttcgccga
    19321 tcaagtcagc aacagagcgt ttgtataaga aactttgacc aaaatcgcct tgcaaagcat
    19381 ttccgatcca acggaagtac tggacatacc atgggtcatt caagccagct gcttgtcgca
    19441 tctgttcgat cactgccgga tcctgatttg gattgatcaa accggtaaat ggatcccccg
    19501 gcatcgcttg ggctaataag aagatcagaa cacttaaaat aataacttgc gggatcatca
    19561 ataaaacacg tcgcagaatc gttttccaca ttagcttgca cctccatctt tcaacgccac
    19621 tttgtgtgta gttgtgatcg tacgcaaatc gtagactcga ccagtcgcat catagtattc
    19681 tttttggttt tggatatact cttgttctac tcttcgtcga ttttctttgt ggctttccct
    19741 attttctaca tcgatttgcg gaatagctga aagcagacgt ttcgtataga tatgtcgcgg
    19801 gtcattataa atgtcttctc ttgtaccgat ttctacaaat cgacctttgt acataattgc
    19861 gatattgtcg cacatgtgtt ttactacacc taaatcatgt gaaatgaata aatagctgag
    19921 gccgtattct tgctggattc gtttcatgaa attcaatact tgcgcttgaa cagatagatc
    19981 cagagcagat acaggttcat cggctacgat cagttttgga ttggtagcaa cagcacgggc
    20041 tactcctaga cgttgacgtt gtccgcctga aaattcatga ggatatttat atagcgcatc
    20101 ttccggcatc ccaacgatat ctagcagtcc ttttaccttt ttcttttctt cttgatcgct
    20161 taaccgttca aaatttcgga tgggttcagc aatgatatcc aacactcgct ttttaggatt
    20221 caaactggac attgaatctt ggaagatcat ctgcacatcc ttgttataac ccattttttt
    20281 gcggttgctt cgcttcgtta catcttttcc ttcatacatg atttgtccgg ctgttacctt
    20341 ttctaatccc acaacggctt ttcccgtagt agattttcca gagccagact ctcccactaa
    20401 accatatgtc tttccttttt cgataataaa atccacacca tctacagcat atacatgatc
    20461 cgttactcga ttgaagaatc cactgcgaat agggtaatga actttcaggt ctttgattgt
    20521 gattaattct gtcatctaca cctctccttt ttcgtctcta aagtggaaat gcttatagca
    20581 ggagcaacgt accaagtggt tcggtgcgac ttcatgaagg ataggctctt cttcatgatc
    20641 gcttgctggt atccatggaa tacgtggtgc aaaccgacaa cctttacgag gcatgttttt
    20701 cagagaagga acgacacctt cgatcacatg gagttctgca tcatgatttc cttcttgtgg
    20761 tatagagttc agtaaagagc gtgtataagg atgcttagga tgatcaaaca gttcttttac
    20821 acctgctact tcaacgaact gtccgccata catcactgcc actcgatcag ccatttctgc
    20881 tacgactcct aagtcatgag tgatcaagat gatccctgtt tttgtttctt cctgaaggtc
    20941 tttcagtaaa tcaagaatct gtgcttggat ggttacatct agggctgtcg tcggttcatc
    21001 tgcaatgacg attggaggtt tgcaggcgat tgcgattgcg atgatgacac gttgtctcat
    21061 accgcctgat agttcatggg gatattggcg tccaactctc gcaggattag gaatccctac
    21121 ttgttctagc aattctagtg cacgagcttg acgctgttct gcattcatct ttgtgtgata
    21181 ggaaagtcct tctttgatct ggtcttcgat tctcatgaga ggatttaatg cagacaacgg
    21241 atcttggaaa atcattccga tatcatttcc gcggatttta ttatagagcg tttcgttcag
    21301 gtcgattaaa ttcagatcgt tatataaaat ctcacctgta atttttgtgt tattaggatc
    21361 aagtaatccc ataatcgtag tagccaaggt acttttcccg cacccagatt ccccaacgat
    21421 tgctaaaatc tcgtttttgt ctaaagtaaa tgaaacgtca tctactgcat cgtaatagtc
    21481 atctttgagt cggaatcctg tatgtaggtt ctgaacttct aataattggt tatcggacaa
    21541 tcgaataccc ctctctgctt cctaaatgga agtttttctt aataaaaaag gacattacac
    21601 agaataaatc gttttataac aaag
//
LOCUS       DARBER010000034        21293 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARBER010000034 DARBER010000000
VERSION     DARBER010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.034"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(30..743)
                     /locus_tag="SCK06_001238"
     CDS             complement(30..743)
                     /locus_tag="SCK06_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1151255.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            918..2342
                     /locus_tag="SCK06_001239"
     CDS             918..2342
                     /locus_tag="SCK06_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1151256.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2641..3348
                     /locus_tag="SCK06_001240"
     CDS             2641..3348
                     /locus_tag="SCK06_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1151257.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3335..3658
                     /locus_tag="SCK06_001241"
     CDS             3335..3658
                     /locus_tag="SCK06_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1151258.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3747..4784)
                     /locus_tag="SCK06_001242"
     CDS             complement(3747..4784)
                     /locus_tag="SCK06_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1151259.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5105..6088)
                     /locus_tag="SCK06_001243"
     CDS             complement(5105..6088)
                     /locus_tag="SCK06_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG1151260.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6503..7273)
                     /locus_tag="SCK06_001244"
     CDS             complement(6503..7273)
                     /locus_tag="SCK06_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151261.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7266..8201)
                     /locus_tag="SCK06_001245"
     CDS             complement(7266..8201)
                     /locus_tag="SCK06_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151262.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8517..10166)
                     /locus_tag="SCK06_001246"
     CDS             complement(8517..10166)
                     /locus_tag="SCK06_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1151263.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10283..12886)
                     /locus_tag="SCK06_001247"
     CDS             complement(10283..12886)
                     /locus_tag="SCK06_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1151264.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12898..13947)
                     /locus_tag="SCK06_001248"
     CDS             complement(12898..13947)
                     /locus_tag="SCK06_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG1151265.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13973..14287)
                     /locus_tag="SCK06_001249"
     CDS             complement(13973..14287)
                     /locus_tag="SCK06_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG1151266.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14303..14749)
                     /locus_tag="SCK06_001250"
     CDS             complement(14303..14749)
                     /locus_tag="SCK06_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1151267.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14750..16672)
                     /locus_tag="SCK06_001251"
     CDS             complement(14750..16672)
                     /locus_tag="SCK06_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEG1151268.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(16884..17780)
                     /locus_tag="SCK06_001252"
     CDS             complement(16884..17780)
                     /locus_tag="SCK06_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1151269.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(17773..18453)
                     /locus_tag="SCK06_001253"
     CDS             complement(17773..18453)
                     /locus_tag="SCK06_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1151270.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(18454..19236)
                     /locus_tag="SCK06_001254"
     CDS             complement(18454..19236)
                     /locus_tag="SCK06_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151271.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19229..20146)
                     /locus_tag="SCK06_001255"
     CDS             complement(19229..20146)
                     /locus_tag="SCK06_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1151272.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(20310..20633)
                     /locus_tag="SCK06_001256"
     CDS             complement(20310..20633)
                     /locus_tag="SCK06_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151273.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         6311 a         4375 c         3539 g         7068 t
ORIGIN      
        1 taagctctga aaaaataatt ttcccgagct tatttttcaa caaaaacagc ttcaaaaaca
       61 aagtcttgat ataaatgtct cgataccgaa tactcgaaag gtttaccaga cgatagagca
      121 acaactttgt ctatctcaat cataaattca cctggatcta tttttaatat ttttgcgtca
      181 aattcattcg cagtatctcc tttgatacgt atgtgcgaag tctttatttc cagacccagt
      241 tcatttgtga tataagcata tacagatttc tttaaatgtt ttggttccaa gcctggtatg
      301 actgctaatg gcatgaatgt ttgctcaatc gagtatggtt gacaatcaat aaaacggctt
      361 cgtacaatat tatatacata gtcttttaca ctgatactta atttatcagc tacttttttc
      421 gatggaagtt caattgagaa agtatggatt tctgaatcta cgtgttcttc accaaccacg
      481 ctaaacaaac cttcgtttgg accaagaact cgtgagttat caattggtgt tctgacgact
      541 tcagtcccat acccacttct acttcgcaaa actccttcgg aaacaagtaa gtctacgcct
      601 ttctttactg ttaacttgct tgtatgataa ataactgcca attcatctcc tgtaggaagt
      661 ttctcaccaa ttttatactc tccatttaca atttttttct ttatatctaa gtaaatttcc
      721 atatacttcg caattttttt caatggtgac cctccaatca aaaacatatc ttcatctata
      781 ctttaacata aataaaccga taaaatccac atcaaaatta tacacacaat aaaaaagata
      841 tatatattta aacaaaacta tttactttaa gttgtaagcg gtgttatcat taagtggaaa
      901 caggaggtaa aaaaattatg acaactatga aaaagctaac ttttccagat gatttttggt
      961 ggggttccgc ttggtcagct gagcaagcag aaggaaaagg agaaacagga aaagcagaga
     1021 ctatctggga tcgctggttt aaagaacaac cttaccggtt ttatcaacga atcgattcct
     1081 ctattacaac cgatcacatc catcaatata aagaagatat agaactaatg aaaaaaactg
     1141 ggcataactc ctttcgcgtt tctatttctt gggcacgaat gtttccaaat ggcggtaccg
     1201 gaaaagtaaa tcccaaagcc atttctttct atcgaaaatt attttccgaa atgaataata
     1261 acggaatcaa agtatttgcc aacttatatc attttgacat gcctgctact cttcaagata
     1321 aaggaggatg ggaatcacgt gaagtagttg atgcttatgt tcattacgct gaaacttgtt
     1381 ttaaagaatt tggtgactta gtttgtcatt ggtttacctt caatgaacca cttgggccta
     1441 ttttaggtac ttatcttgaa gattttcatt atccaaacct cattgacttt aaacgcggag
     1501 cgcaagcagc attcttcact atacttgcac acgcaaaagc aatacaagta tttaaagaac
     1561 tagctcttcc tagtaaaata ggcgttattc taaatttgag tcctactttt cctcgtagtc
     1621 aaaatcctgc tgatgtgaaa gcagcagaaa tcgctgattt attttatacg cggagctttt
     1681 tagatcctat ggttaaaggg aatttttcta aaaagcttat cctgttatta caagaatata
     1741 atcagcttcc tgaagattat acgaaagaag atcttgtatg tatctacgaa aataccgctc
     1801 aaattcttgg actaaactat tacgagccac gtcgcgtcaa atctcgttct tctatggtgg
     1861 atacatctag tccattttta ccagagtggt ttttcgaacc acatatcatg cctggacgac
     1921 gaatgaacga ataccgggga tgggaaattt acgaaaaagg aatttatgat ttgtgcatgg
     1981 atatcaagaa ccattatgga aacatcgaag catttatctc ggagaacgga atgggcgttg
     2041 cggatgaaga acgtttcatg gatgatacag ggcaaataat tgatgattat cggattgagt
     2101 atatcaaaga tcatcttgct tacttatgga aggcaataaa tgaaggatgt aacattaagg
     2161 ggtatcatct ttggacattt attgattgct ggtcatggat caacgcctat aaaaaccgat
     2221 atgggctggt ttcactcgac cttactactc aaaaaagaac aattaaaaaa agtggcgaat
     2281 tttacaagca attaagtgat acaaatggat ttagatacga tgtagacttg ttagttgaat
     2341 aatattctaa aattccactt gaagtgaaca cattagaaaa caagaataat gagaaatacc
     2401 aataacaaat actttttctt catacttcat ttatacttgt cagtataaga gactgtgaca
     2461 aatgtagtga tcctcagaag ttaggtctgg aaatctaact tgagaagatt tttttaaact
     2521 acgtccagtc ttttttattt gtttaaaatt gaagttgaca agtcttcatt aaaaatttag
     2581 aatattcaca agtttgacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
     2641 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgtttttggt
     2701 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccgggtt tcatccgctg
     2761 gtcgtcacgt tgatgtccct gctgatctat gctggttctg cccaatttat cacggtcagc
     2821 atgcttgcta gccatagtcc actgctctcc atcgttctct cgacctttct agtcaattcc
     2881 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
     2941 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
     3001 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
     3061 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
     3121 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
     3181 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
     3241 attctggtat atgtcggact gatttttatc ccaagtaact tagtgatttt agttgtaaca
     3301 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
     3361 gactatttta ggttgtactt tagcaacttg gctttctagg atcctaccat ttgtcttact
     3421 gaaaaaattc gatttgccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
     3481 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
     3541 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
     3601 cttattggtg atcgtcttgg taggaattct ttccctttcg ttgattcgct tattataaat
     3661 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
     3721 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
     3781 cctaatatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
     3841 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
     3901 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
     3961 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
     4021 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgttt gatttgatca
     4081 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
     4141 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
     4201 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
     4261 agttgattca aaatcgaacc gacgccccca gctccgttga tcactaagat ttttttcccg
     4321 cggttggcat tttcttctgg agtcagacca aatttttcaa ataacaattc ataagcggtc
     4381 aatgaggtca acggcaaggc tgcggcttct tcatctgaaa gattttttgg tgcaagagcg
     4441 acaatacgtt cgtcaaccag ttggtattct tgattgcttc ctgctcttgt cgttgtacct
     4501 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt catcacctac agcaacaact
     4561 ttcccgactc catcaaaccc taagatacgt aaagcatttc ttatcccgtt atcttggcga
     4621 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
     4681 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcgacaac
     4741 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
     4801 cttcatcaag ttggagcatc catacagttt tcatttcaat atatatagta tgctatttgt
     4861 tccactttta ttcatcataa ttattctctt acttaaagta aactatttgc acttttttgg
     4921 ttaaaaaaga ggttgcgaca agacttttgt cacaacctct tttaaacggt gttcaatcaa
     4981 ctgaccctcg gtattcgctc aaaaaacgac aaaatatgag gagctatttt tgtttggctg
     5041 ttcttattac ttgatgcagg acagcttttt cacgacctct aattcacggt gttcaagcaa
     5101 gaaatcattg ttcactgaaa agcgttggat gttctttttc aaaggtgtct aagatctctc
     5161 gtacttcatc agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa
     5221 atcctttgat atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt
     5281 ctgaataatg atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg
     5341 tatgttccct cggttctttt tcaaatgcat aaggattctt gaaaatcccc cggccaatca
     5401 tgatgccatc tacgccatat ttttcggcca attcttctcc tgtctggcga tctgggatat
     5461 ctccgttgat cgtgatcagt gtttgcggag cgatttggtc acgtaaagca atgattttag
     5521 ggatgacttc ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag
     5581 aaagattggc tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt
     5641 ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc gccagcttta gccgcttcaa
     5701 tcaattgtgc cgctacttct gggcgaagga tcaagccact gccttttcca cgttctgcta
     5761 cattagggac tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag
     5821 caatgctcat ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct
     5881 gttcatcttc tgtaaaggtc aatcttcctc gtacactgtc ttttccatct ggatgacaga
     5941 aactgtctga gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga
     6001 catggcgaaa tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg
     6061 gcagctcttt ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg
     6121 aagtcgcaat ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag
     6181 tgaacagctt aaaagatgtt tctgattgag caaaaaaaga ctgtgacaaa aatcttatca
     6241 caattccttt tccggaataa atggtgttca aaagcttgca tctgcggtaa taagtccaac
     6301 caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca
     6361 gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca
     6421 acaaacgaca aaatatgagg aactattttc ccgtttgttt ctctaatact caggtcaaaa
     6481 cgctgatttc acaacctctt ttctaacttc tcaatcctct tcctttattg atcaaatacc
     6541 aagtaacggc gtacaaaaca acgatgaata gcacaagtac gatcatcgat gttgctactg
     6601 gcacatcgat tgttccaagg aatccgtaac ggaaaccgga gatcatataa acgatcggat
     6661 tgactttcga aacagcttgc caaaatggcg gcaacataga gatggcatag aaaacgccac
     6721 ctagataagt taacggctgc aagacaaatg taggcacgat cgatacatcg tcataagact
     6781 gagcaaaaat cccgttgagc agtcctgcta acgaaaacaa gattgccgtc atcaataatg
     6841 taatgatgac catgaaccat gaatacacat gaagtggtac aaagaacaag gaaatgatcg
     6901 ttacgagaga gccgacgaga atactgcgac ccactccgcc aatcacaaag ccccaaatga
     6961 tcacatgcgt aggaacaggc gctaccaaga tttcttcgat gtttttctgg aatttttgag
     7021 agaagaagga cgaagaaacg ttggcatacg aactagtgat agcagacatc ataatcaaac
     7081 caggcacgat gaattccata tatgaaaaac cacccatgtc accgatccga ccaccgatca
     7141 tttttccgaa aataacaaag tacaaggatg tcgtgatcac aggcggaacc agtgtttgga
     7201 cccagatacg caaatagcgg ttcgtttcct tgatagctaa actttttaat gcagtaaaat
     7261 aaagattaaa catttttctc ctccgtatga ttattttctt ctgtgatttt caaaaacagt
     7321 tcttctaaac gattcgactt attcctcata gaaagaactt tgattccttg tcttgtcagc
     7381 tgatcgaaga ttccgttcac cccttgattt cgttcaactt cgacagcgat tgtctgttca
     7441 tcttcaaatg tacaagcgta tccttcaatc acaggtttgg tctcatatgg cgctaaatca
     7501 aagataaatg tttcaaattg cagtttggat agaagctctt tcatactagt attctcgatc
     7561 agttcacctg attggatgat cccgatgttg cgacaaagca tttcagcttc ttctaaataa
     7621 tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt ctcggagaaa ctcccacatc
     7681 tcgcgtctca attcaatatc gactcctgct gttggttcat ccaagatcag caaacgtggt
     7741 tcatgcatca atgccctagc aatcatcagt cttcgcttca ttcccccgga aagcatacgt
     7801 gcacggacat ttctcttttc ccaaagattg gactgcttca aatatttttc gctgcgtttc
     7861 atcgcttctt tgcgagagac accgtagtag cctgcttgat tcacgacgat ttgctggacc
     7921 gtttcaaagg gattgaaatt gaactcttgc gggactaatc caatctgttg ctttgctcgg
     7981 actagatccg tatccagatc ataaccgaag acattcactt ttccagatgt tttattgact
     8041 aaagaagtaa tgattccgat cgttgtagac ttcccagctc catttggtcc taatagcgca
     8101 tagaaatctc cttctttcac ggaaagatcg attcctcgca atgcttcgac cccacttcca
     8161 taaacctttt tcaaattatt tatttcaagc gcatttgcca tttatttcct atctccttat
     8221 agacatgatt ctttattgta tcatggaata cagcagatga aaaaacaact tggttccagc
     8281 gaaaaatcag gttgctgggc tcaggaaaat ctcctaatga aagacatcaa tcggacgaag
     8341 tcagacaacc ttttcagaaa aaacaaatcg aagataaagg attactattc atatgtgcaa
     8401 caaaaggagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     8461 acggcggaag caaaagccaa gatttttgat ccaataaata ctttcgatca cttcgcctat
     8521 cccaatacat gccactgccg cttttcatca aataacgcgg cttccgtttt tgcggtttct
     8581 actaaagtct ctgggtaatc caaaactttc agcaaggcaa tcgcatttcg ggtccttgaa
     8641 ggtccttctt ttaatttgaa atcaaaactg attccttttc cttctgttac ttgttcttca
     8701 aagtggacat tctcacaata attctttaaa atctctgtca attcgatatc atgtgtggcg
     8761 acaaatgcta aagatggata ctccgcaagc caccgaacga ctgaagcgga agaagcaatc
     8821 cgttcgatcg tatttgtccc ttttaaaatc tcatctacga agcagtagca gcgttctttg
     8881 gaagccactt ggtccaacaa acgtttgatc gatttgattt ctgaaacaaa ataactgtcg
     8941 ccttcaaata aatcatcttc gattgccatg gaagtcaaca catgtccagg ttgcagggta
     9001 aactgttctg caatcgctgt ttggatcgtc tgtgctagaa tacagctgat ggctatactt
     9061 ttgacatacg tagattttcc tgatgcattg gaacccgtga ctaatgtatt cttctgccag
     9121 ttgaccggat tcactaccgc ttcttttaac aatggatgat agatatcttt cgctgtcaca
     9181 ccgccctcag taaactctgg tcggcaagtg atgggcatat aagtacggta attcaatatt
     9241 gcagcggcta cttctaattc tcccagcaat tcccacagct tgatggcttc agttgaatgc
     9301 ttttctaatt tagtgagaac gaaatgatag gagataaatg gcagcatgaa catgatgttg
     9361 acatattcaa aaatcaactc tgcttcactc ccactcttca tacgaaatga aaaacctaat
     9421 ttagggatgt ctcttaatgg tttagtttgc tgtttcagct cttcttgaag tggtgtcggc
     9481 agttttgcta gctgattggc ggctgaaatc gttgaaacca aataactcat gctattcagc
     9541 tctgtttcta gtgtttgttt cttcactaga taatagaccg tattgaatac taacccaccg
     9601 attgccaaat agattcctag tacttgtcct aaaagaagca aacccaaacc caccagcgga
     9661 agcaagccaa gaaaaataaa taaacctacg cgtccaatct ttttactttt tccatccgct
     9721 agatattgtt ttgcaaaatt atgatctttt tttcctaaac gagcgaattg atattggatt
     9781 ttttctctca gttgaggatt ttcctgataa aaagcaatca gtttttctaa ctgctgatct
     9841 tcaccaaaat caaagctgcg taaacgttga tacaacgctt ctgaacctac actagaatag
     9901 gtactgttga ttccttcgaa tacttcgaac atatccaggt cataccaagt aagatcatcg
     9961 atttcgctat cccatgaacg gaacttcttc tccgtgagcc atgcttcctt caagctttct
    10021 tctttatcaa atctcggttg ataaggaatt ttttcccatt tgctacgaac tgtttccttt
    10081 aatttcaaac gtgaaaaaat gtctaaagca actaataaaa taacagctgc aataataatt
    10141 aaaacgataa attctctttg acccatatcc ctctccgttc ctttatttgt tagctttagt
    10201 atagcactgt tgcgctattt gaaaagaggg taaaaaaaca cctttcaaaa taaaaggtgt
    10261 ttttttcttg tatattacat atttatctct catttatcaa aattgacaaa taatcatagg
    10321 gaggaattgt tgttaattct tgtttattct ccagtgcatt gacaactgtc ccaagggaaa
    10381 ggatagcctc aggtactatt ttttcttgtt ctgttggatt agctaagcga agaacaaatc
    10441 cttgttcatg aaaataagaa ggataaatcg atgatacaat taagtcttca ggtaattcaa
    10501 tcaatgaaaa tttacgagga acatcaagtt cattttgtaa tggccaaatc ttgttatcta
    10561 acctgtgaat aaatacattc agtttctgct tttgataaga aatagattgt tctaaccgtt
    10621 tttctgcctg ttttgccact ttgaactcat taaatcttcc ttcttctaca ttaaatccaa
    10681 aagtgacttt atactcacct atctcttgag ctagcggtgt ttccatcatg acatgccctt
    10741 catttgtcgt atctcctgaa gctcgcccag gtcgccaagc aagattaggt tttccaagtt
    10801 cccctgttgt tgaaaataag gttatgatta attgattttc tatacgttca tactctttta
    10861 aaccatctgc ataaaacgta aaataatgat cattttctgc cacagagaca ctcttatcga
    10921 atatttctaa attaacaggt ttctctacca tagtttctga ccaattcgga ggaatgacat
    10981 caggggtatt ttcaataaat ccgttttgaa tttgtgcaat tgatttttca gatttatcta
    11041 aaacattaat tgctaagcgc atacgatgcg agtaaacagt attatgaatt ttgagtgtgc
    11101 cttcaacgat ttgactttct cccagactga acgatatttc ataagatatt tcttccattt
    11161 cgggtttttc agataaacga tcttttaaat ctttaggaag ttgagctttt ccgtaaacaa
    11221 ctaatgtttc ttgtaaagaa tctttatata ctttagctgt ttctaatttt agagatagtt
    11281 ctgtatctcc ttctaatggt gaataatcat acgtatcgcc atcatttgct gaatcgatca
    11341 aatgaacgaa atcaagatat tgtctatctc caactatcaa attcaattgc ccatctttaa
    11401 aagaaagttt atatttattg ttacttatct cagttttgtc tatctcgatt gctgtttcta
    11461 atttttcatt atttttctca aattcaacaa cagtgtatcc tagtgctggc acatttacat
    11521 ttatttttat gtcaagttca taataagctg gttcatcaaa atattcgaaa cctgtagcaa
    11581 tcatccgata agcatttttc cgttctggaa aataaacttc tttttcaatg atattatagg
    11641 gactaccttt gaagtgtatt ttctttgaat cagctactac atgcacgatt ttcgatccac
    11701 tataattctc agtcgttgtg ttgaaaacaa ggacttcatt cgattttagc tttaatgcat
    11761 cagccatccg ttttacaata aggttttcga ttccgtcagc tatctcatta gcttctttga
    11821 ttcgatggaa aatatcttcc gcaacattat ctgaaacaca gccaccaata ctatcatgag
    11881 cctggttttg caaaatcttt ttccataacc gacgaagtag gcctttactg atatggatac
    11941 catttctttc tgcaatcagc atgagcggct caactcgttt tagaattttt tgttccaatt
    12001 taaaattttc tattttcatt tttgttctta ctgagccaat agaacgatga acacgagagt
    12061 aaaccggctg acgaagttca cctttatatt caggtaattc ttttttgttt ctgataactt
    12121 ctacataaga aggataatct gaaataatat tgtggaattt actgtattta ttcaacttct
    12181 ctacgacttg atcaaaattt aaaatcattg acttttgatc tacacccgaa ggtactaaaa
    12241 tatctttgtt ttttccttgc atatgaagca tttcaactgc ttcatccaac ttttcttttg
    12301 caaattcagg aatatcttta attgcgtcta aggtcatcat tccagtcata taaccaaatg
    12361 gaaaattcat ggcataaaca aatttatctc ccaatccttt ccagataaaa taaggagatg
    12421 gaaccatcgt ttcaaagttt aatcctcgcc aacacatgaa attatcgatt tctacttgat
    12481 gcaaaagtgt aggcaaattc gcattgaacc caaacgtgtc aggcaaatac cctaccatca
    12541 ttggatctcc atatttaagg atcgtatcac tgattccaat cattaagttt cttaaaattg
    12601 attcagcatc cactaataag gcatctgttt gtgcatacca cggtcccacg aatagttgtc
    12661 ctgttttcat cagtcgttgg ataaccttaa gcttttgcgg attgatctcc aaatactcat
    12721 cgacgattga agtttgtcca tctaaacaaa agtttacttt agggttcctt tccaattcat
    12781 ctagaacttc agtaaagacc tgatcagaca atacgatggc atcttcattt gtaaaatacc
    12841 attctctgtc ccagtgcgtg tgttgaatta tatgtgcatt taccatatca gattctccta
    12901 ctttccttct tctactgaat ttacttgttg ttctttttta tacttgatgg attttagtgc
    12961 attgaccatt aacgctgtaa ccactccccc aataacaatt gatactaaat ataaaggtat
    13021 attcgtaaca aaaggaattg ccataaaggt tgaaagcatt gtttcttgag taacaccaaa
    13081 aaatccattt agaccacatg ctactgcagt gcccaccacg atgcttggta ttactcgtaa
    13141 aggatctttc acggcaaacg ggatcgtccc ttcagtaatc cccacaaggc ccataatcat
    13201 agctgtcttt gcattatctc gttccgattc atcataaagt tttttgttta aaattgttgc
    13261 taaaaacatt ccaaatggcg gaatagcaat tcctaataac atggctgtac taggtccaaa
    13321 gcttccgctt gaaaaagaag caaccccaaa agcatatgcg attttattga taggtcctcc
    13381 catatcaaac gccatcattc ctcctaaaat agccgacatc aaagcagcat ttgctcctga
    13441 catgccttct agccaattag tcagtcctgt tgtcattgct tgaataggcg tagctagtac
    13501 ataccataat atgattccaa caactaatgt agtaacaaaa ggaatcagca taattgattt
    13561 tagactaata acttgcatcg gtacttttac ttttttcaat gcattagcta aatagccaca
    13621 agctaatcca gcaaccattc ctccaataaa tcccatacca agattttgac acatcatccc
    13681 agtgataaaa gctggtgcta gaccgggacg gtcagcaatt gaatatgcga tataggctga
    13741 aatcatcggt acgataaact gtaatcccgt ctgaccaatc attcgcagga ctccccagaa
    13801 tgtatccgtc tggtcccaaa cacctgtgcc accaaaaatg actgcaatag aagttagtaa
    13861 ccctgctgct acgacaagcg ggatcatata ggaaacccca ctttggaaat gatttagaat
    13921 ttctcttcct tgtttcttga attccattag taactccccc acgatatatt ttttatttgt
    13981 taattaatgc aattgcttca tcaatcgtct cttcaccatc ttttactacc tctgaggtcc
    14041 ctactgttaa aatcggttta ccgtcaaagc gacctcgttc tgaaacattt gtatctactg
    14101 caaatatgac aacatctgcc tcggaaacat ccgaaattga aagtttgttt tctatcccca
    14161 tagccccctg tgtttctact ttaatatcta accctttctt cttcgcaaac ttttctaaat
    14221 tagcttttgc catataagta tgggcaatcc ctgcgataca tgctgttact gctactattt
    14281 ttgtcatatg aaattcctcc ttttaaaatg atagagcttt taccaattct tttttatttc
    14341 tcgaatcttt tattctttta acaaactcgt catccatcaa tcttccagat aattctgcta
    14401 aaatacgtaa atgattagta cctttatcag tgtcttttat tgctaacaaa aagacgagat
    14461 caactggttg atcatctaat gcgccccatt caacagaccg ctttaacttg gctacagcca
    14521 ctgttgattc aattactgct aggcttttgc catgaggaat ggcgatattt ttcccaattc
    14581 ctgtggtcat atggttttct ctgtcaaata cgtctttttg aaattgtttg tggtcagaca
    14641 caaacccatt agtctctaac aatttcgata atttttcgac tacacttttt ttatcagatg
    14701 catttaaatc tatcactata ttatttatat tgattttttc actgatcatt tataagcctt
    14761 ctttcaatat ataaactaat ttttcaaacg acgaactcgt ttttaaattt gctaataact
    14821 catgattcga agcgatttca ttgaatgttt cataaatttt attcaattca ggttttactt
    14881 tttcattaag tgcaaaaaag aaaacagaat gaacaagtgt cccatcccaa tcaattcctt
    14941 ctggattaac gtagacacag attactggct tttttataaa atctacttcg atatgcggca
    15001 tagcaaaatc attcatcgct gttgaagata gtttttctct tttgattgct ccctctagta
    15061 tcccttcttt cgcgtaattt tcttttacca attttttggt aattatattt aaaacgttca
    15121 cttgattatc ctttatttgg tgggtaaaaa agcaaaatcg tttatctaat aaatttagaa
    15181 aagcatcttc tgtttgttca tttaactgta gatactgttt tacttttctg acatctgccc
    15241 ctaacatcaa aggactaaca acaataacag gaaaatttgt attttcaatc ggaatcgtgc
    15301 ttataacaag ttcatcagtg acttttctat cttgtagttc attgatgctt agtacatctt
    15361 ctatctcaac catatctgca aaaccgttat ttattctttg ctctaataat tttgatgttc
    15421 catatccgct actacaaact aatatcactt tcgttttgtc aggtttgtaa cgatccaaaa
    15481 gtgattgaat atggagtgtt acgtaagcga tttcatcttc attaaaatgt atacagaatc
    15541 tctcttcgat ttcctctaat aaatctacac ttatcataaa tgacctttta aaagaatatt
    15601 tgattttgtc cgtataaggg ttatagatat ttactcccaa tttgagtctt ttaactgctg
    15661 cattgagatg taatactaga ttattgatca attcttcatc aggataaaat tcatttaatt
    15721 taattcttac taactcttgt agctcaacac ccatatctaa ataggaaagc ttcgtttgat
    15781 ctaagtcgtg attcatagca tttttttcaa tcgaagtaat gtgaatgtca acataagctt
    15841 gttcatctat tggcacaata attgaaaatg ctttttcaat cattttgatt aaaataagtg
    15901 tattctttga gatttcttct tcagaattat tactacgtga taagaaattg cttgtttcaa
    15961 tcctttctat cgcaatagca aggtggatag ctaaagtttg aaattcataa tctgtaaatt
    16021 tcaattgtgt cttattcatg tatcgattaa gcaattcggc tacttttttc aatatgtcct
    16081 tatttaagaa attattcgct tcaccaatga aattgatatt caagtctaag agatctgttt
    16141 tactgttaga tgctgtaaag ccaccccaat aatagttcac aacttctgac atcaaccttc
    16201 ttttactttt ttcatcagct ttgatgcgca cgccatttcg atctgaaact aaggacatcc
    16261 catgcttgtt tagtcttttt ctaacatctt tcattgtgct ttctaccgtc gacctgctca
    16321 cgtataattc gtcagcaaga ttttgaatct ttagagaatc attagtattc aataatttag
    16381 caaacacata agttagacgg tcctccggag agtctgcgat attgatttta tgactggata
    16441 ataaatttcg aagtgtttct ttttttccaa taagcgaaac gcctttttta ggttgaacca
    16501 acatttgtac atctggcatt ttttggatta tttctttcaa aattaaaata tctttgctaa
    16561 ttgttttcgt agatacatat aattgttctg ataattcttt gtaagttgtg tgctcatttt
    16621 caatcaaaaa gtatagtatt ttcatttgcc gttcgctaaa ttcgatgccc attatcttca
    16681 cctcctgaat acagcttaag tgtacaatta ttatctctat tttcatacag aagaaagttc
    16741 ttgattaatg tagaagtttc taatattctt gattatttca aatcaataaa aaagcctgca
    16801 atatttatat cacaggcttt tttctctatt aaatatatat aagataatac atttttctac
    16861 tagttctcac taatcttgta taattaaacc ctccgcaact tgacggagat ggagaagatg
    16921 cccccgtcta atctagcggt catctctcct cccattcctt ccaccaattt ttttgacagg
    16981 taaagcccga ttccgctaga ttccgtattt gtgcggctgg tattttcggt ataaaaacgc
    17041 tggaagactt tttcaacaga ggtttgcgtt cctggtttga taggattggc cactgttaat
    17101 cgaatgaatg gatcctcttt tgaaaatgtc acctttgctt gtccatctgc ataacgcaat
    17161 aaattctgta tgatattttg cagcactcgc agcaacatga gcggatctgc taatacagtt
    17221 gtatttatcg agtgttcagc gaacgctacc tcgatatttt tctcagaaaa ttgttcataa
    17281 aaagagaaaa atacttcttc cacctcttta gaaaaagaaa gcgtatggag atccatttct
    17341 ttttgcttcg tctctattcg agcaagatca taaaactgat ccgtcatact gagtaatctt
    17401 gcaacagatt gtttgatgat cgttaaatac ttctcccgtt tctccgtatc ctctacctgt
    17461 tccagtaatt gtacataacc gttaatagca gtcaaaggag ttctgagatc atgagaaagg
    17521 ccgacgatcg atagtctgat gttttcttcc atttcttgaa atgcttgtcc tttttgttca
    17581 ttttcatcca accatgtatt cagtgcatct actaggtcca ttagattctt ttctcgaaat
    17641 tcgactgaaa gacgaccacc atattcttgt cgttcagaaa gagtggcgat tttcttagtt
    17701 aatctcttga tttccctttg caggaagaaa ataaaacttc ccataaagat aacaacgaga
    17761 agcaacagcc atttaatcaa atctaaaccc cactccccac accgtatgaa tatacacagc
    17821 atgtgccttt gctagcttcc ctcgaagatt ggaaatatgc atattcactg ttttctcact
    17881 atcaaaatat ggttcttgcc aaactgtttc ataaagattt gctcgcgaaa atactttttt
    17941 cggatgttct aaaaaaagct tcagcagatc gaattctctt cctgtcaaat ggacagccac
    18001 accattcaca tttacatccc tcgactcttt gtccagtaag atttccttgt aatagatgcg
    18061 ttgcgacaga tcttttactt ggacagaagt ctgatgacga agttggatgc cgattcgtaa
    18121 caaaagttct tcgatatcaa atggtttcgt cacatagtca tccgctcctg ctgccaacaa
    18181 ggaaacttta tctctttgat cgctttttgc tgaaagaacg atgatcggaa cattcgatgt
    18241 ctgtcggatt tctgctaaca atgcttcacc tgaaagccct ggaagcatga gatctagaag
    18301 gatcaaatcg acaggatatg tgcttaacaa taattttcct tccgtacctg aataagcatc
    18361 aagaagtttg tatctctgtt caagaacctc ctccagtaag gaatgaacag cttcatcgtc
    18421 ttcgataatc aaaatagttt ccagcaggac cacctactta atgtcagatt tttggaaaat
    18481 aaacattcct agagctgatg ataccacgat tgttactaga ctgaccagaa tcattttcgg
    18541 tgcttgcgaa taagttgctt gttgcgcaaa gtccgtatac atgagccacg tttccgtaaa
    18601 ccactccgtt agaaattcca agcctttgat ttttgcgaaa acaccgggaa tgaaggccgt
    18661 tcccatgata taaagaaaag aaagaagcaa actgacactg gtacttctag tgatgaatgt
    18721 caaagccact ggaacagata atgtccctaa tgtcagcaga agcaatacag gaaatacttt
    18781 tagaacataa tttccatagt ttttgattcc ctcaacttca ccaaatccga aagcaagtgt
    18841 gtaagcaagc atggctagtc ccgtgatcaa aaccacgcca agtgttgtga tcaaaagcgt
    18901 aacgagaagt ttcgacacat aataagtcat tcgattccgt cctaaagata acgtattgcg
    18961 aatcgtaccg ttttgaaatt cttccccaat ataaaagcac gcaaatgctg cgaaagccag
    19021 aagaatccat gcagccattt tttctaccat taaaatccct gttgcaccgt tgatgactgt
    19081 atccgttgct tggtttaatt cattgccgta ttttgtatgc atataccatt cgttccaaga
    19141 ataaaaaagc ggaagtacga ccatcagtcc tagtgagacc cacattttac ggctcttcca
    19201 tacttttatt ttttcagcag ttaatagatt aaacatgtgg attgcctcca gttaattgta
    19261 agaaataatc ttccagtttt tgcccaacta ggtgaatacc atccacagga acttttgctt
    19321 ctaccaatgc ttgattgatg gctgctactt ggtcaattcc ttcaaaaagc tggataacac
    19381 ggctagattg tacaaaataa tccctataac caagagagtc gagaaccgtt actgcttgtt
    19441 ccgtcgccga agtgtcaatt tttataaatt gacgagattc ttgtgccaat tcttctttcg
    19501 ataattcttt gatcaagtgt ccttcatgca aaataccata gtgagtggcg atctgtgaca
    19561 attcatctag caagtggcta gaaagcaaga cggtgatgcc ccgttctttt accaaacgtt
    19621 ggatgatttc tcgcatttct gccatccctg ctggatccat cccattcgtc ggttcatcca
    19681 aaatcaaaaa ttctggattc gtgatcagtg catttgcaat agctaaacgc tggcgcatac
    19741 ctaaagagaa attctttgct ttctttttcc ctgtattttc caatcgcatc attttcagca
    19801 ggtcttcgat ttctctatcc gacacaccgc cgttcgctgc ttggatacgt aggttatctc
    19861 ttgcagttaa ttctggataa agagcagggg tttcaatcga ttgacccata cgtcgtcttg
    19921 ctgcctgcaa atccgtcgtt ccaaataatt cgatcgaacc ttcatcaatc gagatcaaac
    19981 ccataatagt tctcataaag gtagacttac ccgctccatt ttccccaatc aatccataga
    20041 tcattccacg tttgatttcg attgatactt tatctaatgc tttggcagca ccgtattttt
    20101 tggttatacc agttgctttc aagaccgttt catttccaaa atccatactt atccttcttt
    20161 cttattgatg agtgaagtat agttgacggg tataaagaaa gcagccagaa atgtataaag
    20221 aaagtgtaaa ttttccaatt gaatgtcaaa aaaaacaatc ggacaatacg tgtctttcct
    20281 attatcaggc agtttgttat acgcttgtat cagtttcctg attctttctt ttcgaccaaa
    20341 gtcgtttctc tgtattcctc ttcactgaaa atttttgctt ctttcagatc aagcttgagt
    20401 aattttacca gaccggttat aaggataact atagcaagac tccctcccag aaaaattaga
    20461 ctgtctcgca gacgtggttg cgttgtcttc caatcaaaga gcgcaccaaa cagtgctaat
    20521 ccaagtggac tggccaccat ggtaatcgtt gttatcgttg acataagtcg tccttgtata
    20581 tgcgttggaa cttctcgctg aagccagacc atataaaaag caaaagaaaa cattaatcct
    20641 taaaaacgga cttatgaaaa ataatacatg ccgtttggga tttacaatta gctaccaaat
    20701 aagaggttat gacaaaagac ttttcacaat cccttcttcc gaataaacgg tgttctcctg
    20761 ccaactcctc ggtcacaagt cacaatattt atcaaacatg agaagcaatt tttgtaaatt
    20821 ccttcttatt tcttgaagct gaccacttct gtcacaacct cagaagcgga taaataatat
    20881 tgtttgatga tactttatct ttttcccgtt tgtttataga tcgaatgata ttatagacca
    20941 agaatcctac ctattaacat agctaaaaac acaaaaaagg cactttccaa attgtggaaa
    21001 gtgccagaaa agttaggatt aaggtaactg cgctcagcat tgcttcgccc agtacgattc
    21061 tcgtaactcg tgaactcgaa cgataatcgc aattaacgtt ttgagaactg tgaagcttta
    21121 cgggctttct taagacctgg ttttttcttc gtatcattga ataagtttga aaaaccttga
    21181 tttataaagg ttttcccgta aatatatttt attatatttg attaaatttg aaatgtttgt
    21241 gttctaaaat gtgttctaat ttaaaggcag tatatttcgt attttagatt aaa
//
LOCUS       DARBER010000035        20485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARBER010000035 DARBER010000000
VERSION     DARBER010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.035"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="SCK06_001257"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="SCK06_001257"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG1151274.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="SCK06_001258"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="SCK06_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG1151275.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="SCK06_001259"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="SCK06_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG1151276.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="SCK06_001260"
     CDS             2857..3732
                     /locus_tag="SCK06_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1151277.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCK06_001261"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCK06_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG1151278.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="SCK06_001262"
     CDS             4629..5063
                     /locus_tag="SCK06_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG1151279.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="SCK06_001263"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="SCK06_001263"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG1151280.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="SCK06_001264"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="SCK06_001264"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG1151281.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="SCK06_001265"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="SCK06_001265"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG1151282.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="SCK06_001266"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="SCK06_001266"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG1151283.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="SCK06_001267"
     CDS             13334..14260
                     /locus_tag="SCK06_001267"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG1151284.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="SCK06_001268"
     CDS             14442..15335
                     /locus_tag="SCK06_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG1151285.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="SCK06_001269"
     CDS             15469..15594
                     /locus_tag="SCK06_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151286.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="SCK06_001270"
     CDS             complement(15728..16360)
                     /locus_tag="SCK06_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151287.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="SCK06_001271"
     CDS             complement(16508..17050)
                     /locus_tag="SCK06_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG1151288.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="SCK06_001272"
     CDS             17185..17574
                     /locus_tag="SCK06_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151289.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="SCK06_001273"
     CDS             17578..18342
                     /locus_tag="SCK06_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1151290.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="SCK06_001274"
     CDS             18471..19418
                     /locus_tag="SCK06_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1151291.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="SCK06_001275"
     CDS             19548..20288
                     /locus_tag="SCK06_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151292.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DARBER010000036        20011 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARBER010000036 DARBER010000000
VERSION     DARBER010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.036"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..152
                     /locus_tag="SCK06_001276"
     CDS             <1..152
                     /locus_tag="SCK06_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151293.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="SCK06_001277"
     CDS             complement(230..763)
                     /locus_tag="SCK06_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG1151294.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCK06_001278"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCK06_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG1151295.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCK06_001279"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCK06_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG1151296.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="SCK06_001280"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="SCK06_001280"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1151297.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="SCK06_001281"
     CDS             complement(4488..5066)
                     /locus_tag="SCK06_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG1151298.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="SCK06_001282"
     CDS             5388..5858
                     /locus_tag="SCK06_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG1151299.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="SCK06_001283"
     CDS             complement(5966..7192)
                     /locus_tag="SCK06_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1151300.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="SCK06_001284"
     CDS             complement(7946..8146)
                     /locus_tag="SCK06_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1151301.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="SCK06_001285"
     CDS             complement(8280..8888)
                     /locus_tag="SCK06_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG1151302.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="SCK06_001286"
     CDS             complement(8973..9443)
                     /locus_tag="SCK06_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG1151303.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="SCK06_001287"
     CDS             complement(9491..10405)
                     /locus_tag="SCK06_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1151304.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="SCK06_001288"
     CDS             10551..11354
                     /locus_tag="SCK06_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG1151305.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="SCK06_001289"
     CDS             11532..12479
                     /locus_tag="SCK06_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG1151306.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="SCK06_001290"
     CDS             complement(12547..12840)
                     /locus_tag="SCK06_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG1151307.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="SCK06_001291"
     CDS             complement(12868..13533)
                     /locus_tag="SCK06_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG1151308.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="SCK06_001292"
     CDS             complement(13673..14305)
                     /locus_tag="SCK06_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1151309.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="SCK06_001293"
     CDS             complement(14312..15379)
                     /locus_tag="SCK06_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1151310.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="SCK06_001294"
     CDS             complement(15376..16107)
                     /locus_tag="SCK06_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG1151311.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCK06_001295"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCK06_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG1151312.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCK06_001296"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCK06_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG1151313.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="SCK06_001297"
     CDS             18270..19439
                     /locus_tag="SCK06_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1151314.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            19764..>20011
                     /locus_tag="SCK06_001298"
     CDS             19764..>20011
                     /locus_tag="SCK06_001298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151315.1"
                     /translation="MIIEINNEILEKNVYEYLIVTLFIPLSLFTVTKGPPCFSTVAFF
                     ELTVTVNPLYRNTGKTIVLPFEDLNVNVISSLFGEKLL"
BASE COUNT         6053 a         3996 c         3193 g         6769 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat a
//
LOCUS       DARBER010000037        19821 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARBER010000037 DARBER010000000
VERSION     DARBER010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.037"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SCK06_001299"
     CDS             6..305
                     /locus_tag="SCK06_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1151316.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="SCK06_001300"
     CDS             378..1739
                     /locus_tag="SCK06_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG1151317.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="SCK06_001301"
     CDS             1761..2021
                     /locus_tag="SCK06_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151318.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="SCK06_001302"
     CDS             2035..2787
                     /locus_tag="SCK06_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG1151319.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="SCK06_001303"
     CDS             complement(2881..3486)
                     /locus_tag="SCK06_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1151320.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="SCK06_001304"
     CDS             complement(3539..4513)
                     /locus_tag="SCK06_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG1151321.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="SCK06_001305"
     CDS             complement(4891..5610)
                     /locus_tag="SCK06_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1151322.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCK06_001306"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCK06_001306"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1151323.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="SCK06_001307"
     CDS             7161..8558
                     /locus_tag="SCK06_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1151324.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="SCK06_001308"
     CDS             8639..9079
                     /locus_tag="SCK06_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1151325.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="SCK06_001309"
     CDS             9227..9553
                     /locus_tag="SCK06_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1151326.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="SCK06_001310"
     CDS             9554..10303
                     /locus_tag="SCK06_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151327.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="SCK06_001311"
     CDS             complement(10383..10838)
                     /locus_tag="SCK06_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1151328.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="SCK06_001312"
     CDS             complement(11265..11540)
                     /locus_tag="SCK06_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151329.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="SCK06_001313"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="SCK06_001313"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG1151330.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="SCK06_001314"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="SCK06_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1151331.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="SCK06_001315"
     CDS             14770..17436
                     /locus_tag="SCK06_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1151332.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="SCK06_001316"
     CDS             17800..18552
                     /locus_tag="SCK06_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1151333.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="SCK06_001317"
     CDS             complement(18574..19728)
                     /locus_tag="SCK06_001317"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG1151334.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6329 a         3472 c         4060 g         5960 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa atggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DARBER010000038        19549 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARBER010000038 DARBER010000000
VERSION     DARBER010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.038"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            29..1168
                     /locus_tag="SCK06_001318"
     CDS             29..1168
                     /locus_tag="SCK06_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG1151335.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1161..1961
                     /locus_tag="SCK06_001319"
     CDS             1161..1961
                     /locus_tag="SCK06_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1151336.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1961..2788
                     /locus_tag="SCK06_001320"
     CDS             1961..2788
                     /locus_tag="SCK06_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1151337.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2766..3500
                     /locus_tag="SCK06_001321"
     CDS             2766..3500
                     /locus_tag="SCK06_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1151338.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3600..4466
                     /gene="rfbA"
                     /locus_tag="SCK06_001322"
     CDS             3600..4466
                     /gene="rfbA"
                     /locus_tag="SCK06_001322"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG1151339.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4480..5052
                     /gene="rfbC"
                     /locus_tag="SCK06_001323"
     CDS             4480..5052
                     /gene="rfbC"
                     /locus_tag="SCK06_001323"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG1151340.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5074..6102
                     /gene="rfbB"
                     /locus_tag="SCK06_001324"
     CDS             5074..6102
                     /gene="rfbB"
                     /locus_tag="SCK06_001324"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG1151341.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6200..7051
                     /gene="rfbD"
                     /locus_tag="SCK06_001325"
     CDS             6200..7051
                     /gene="rfbD"
                     /locus_tag="SCK06_001325"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG1151342.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7086..9119
                     /locus_tag="SCK06_001326"
     CDS             7086..9119
                     /locus_tag="SCK06_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151343.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9163..10443
                     /locus_tag="SCK06_001327"
     CDS             9163..10443
                     /locus_tag="SCK06_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG1151344.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10653..11459
                     /locus_tag="SCK06_001328"
     CDS             10653..11459
                     /locus_tag="SCK06_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151345.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11471..12691
                     /locus_tag="SCK06_001329"
     CDS             11471..12691
                     /locus_tag="SCK06_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151346.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12681..14267
                     /locus_tag="SCK06_001330"
     CDS             12681..14267
                     /locus_tag="SCK06_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1151347.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14306..16444
                     /locus_tag="SCK06_001331"
     CDS             14306..16444
                     /locus_tag="SCK06_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1151348.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(16812..17795)
                     /locus_tag="SCK06_001332"
     CDS             complement(16812..17795)
                     /locus_tag="SCK06_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG1151349.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18173..>19549
                     /locus_tag="SCK06_001333"
     CDS             18173..>19549
                     /locus_tag="SCK06_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1151350.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNE"
BASE COUNT         6196 a         3273 c         4034 g         6046 t
ORIGIN      
        1 atgactctta attaaagtag gtgcccatat gcttgtttct tttagtatgg ttataagatt
       61 ttttctgaca attttattat cactgatatt aacacctgta ttcaaaatca tttcagtaca
      121 aacaggtatg gtcgacaaac caaatgaaag aagaataaat aaagttccaa tgccttcagc
      181 gggcggactg cctatcttta tttcatttgt tatatcgact ttattcttat tccgtaaaat
      241 tatccctcaa tcgtatattg taccgatctt gattgcttct ttagtaatca tagtcacagg
      301 agtgctcgat gataaatatg agctgacacc taaacaaaaa agtataggga tcttgattgc
      361 ttctcttatt atttattttg tagcagatat acgtattgat tccttcacgc ttccttttat
      421 tggatatatc caactaggct ggcttagttt tcccgtaacg attttttgga tcttcggtat
      481 cacgaatgcg gtcaatctga ttgacggttt ggatgggctg gcggcaggga tttcattgat
      541 cggtttaatt acgataggaa tcattggata ttttttcctc catgcttcta cagtgtatat
      601 tccagttgtt attttctgtc ttgtagcaag tattatcggt ttttttcctt acaattttta
      661 tccggcaaag atttatttag gcgatacagg agctctattc ctagggttta tgatggccgt
      721 cctttcacta caagggctga agaacgtgac ttttgtttca tcgatttctt tgctgatcgt
      781 catgggagtg ccggtaacag acacgttttt tgctatcatt aggagaaaag caaaccgtgt
      841 ttctttttct acagcagata aaaaacatct tcatcaccga ctaatcagtt taggctttac
      901 tcataaaggt gctgtcctga cgatttatgc gatggctttg atgttttctt tcacagctat
      961 ggtcatgaat tatactggaa gaatcggaac gatcgtattg attattgcca tgttgtttgc
     1021 tgcaatttta ttatttgaat tgattggtct gatcaatgag aaacatcagt ttattttaaa
     1081 aacattgcga tttttaggga ataaagagta ccgtacgcaa gtaaaacaga aatatttgaa
     1141 gaaatggaac cgtcatatag atgaataaca aattaaacca gccgatcctt attagtattg
     1201 tgacggcaaa cagtgataaa atttttcgga cactagataa ttttatttca actgtaaaag
     1261 atgaaacagc agtaaaaatc cgaatttttg acaacaattc acttcccgag tacaaaaaaa
     1321 gactaacaga gtataggaaa tatccctaca tcgatatcta ttttcatgat gaaaattccg
     1381 gatttggtta tggacataat cacaatctga tagctagcga ttctcgctat ggcattattt
     1441 gtaacccaga tattttagtg gatgaagaga cagttacagc attggtcttg ttattgaaag
     1501 aacatccgga atgtgcgatg ttagcaccta agattcttaa tgaagatgga acaactcagc
     1561 acctcatccg tgagcagctg gctgtttttg attacgtttt gcgctttgtg cctttcaaat
     1621 gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga atgccgagga ttgcctactg
     1681 atgctaatag ttatgtacgg atgatctcag gtagttttat ggtagctgat attgataaat
     1741 ttaaagagat aaaggggttt gatgagcgtt tcttcatgta ctttgaagac aacgacttgt
     1801 gtttgagttt tgaaaaagca ggttacaagt tattgtacac acccttttat gcagttatcc
     1861 atttttacgg gaagggtgcg catcgaaatg caaaactgtt tcgcgtgttt ttgacttcta
     1921 tgatgaaatt tttcaataag tgggggtggc gttttttctg atgaaaccag aaatattagt
     1981 agtcgttgtg ctttatcagc aactattcag tcaatctcca gcttatggac cattaagcaa
     2041 agcacttagc gaaaagcaaa ttcagcttat tatttacgat aatagtcctc aaaggcagca
     2101 aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat gatccgcaaa atcctggttt
     2161 agcggtggct tataattttg cgttgtctca agcagatgaa ggaacacgtt gtctcgtcac
     2221 tctggatcag gatacgaggc tagtcgacga ttattttgaa ctattgcgta aagtaacctt
     2281 tacagatgaa tgtgttgctg cagttccgat gattttttct gggagccgac aagtttctcc
     2341 cctatattca gatcgttata tcaatcgtac agctcaagca gtagaagttg aaggcccaac
     2401 agctgaacga atcatggcaa tcaattcagg tgttgcctgg tctgtcaaat ttctaaaaaa
     2461 gattggcggg tttaatcctg ctttttcttt agattttttg gatcattggc tgttttggaa
     2521 agtggatcag ttggaaaaaa ctgtggagat ccttcctgta cgaatggaac atgatctttc
     2581 ggttttagac tatgaaaaag tttcaagtaa tcgataccgt tctattttac aagcagaaaa
     2641 tcgattttat catgaatatg atcaaaggca tttatccagt caccgacgac aattattgtt
     2701 aagaacagct aaacagttct tgaccgtcaa aaaccggcat atctggcgga tgactttgcg
     2761 ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc ggtttgtata gctaccttta
     2821 atggaggcag atatttaaga gagcagttag atagtatttt atctcagctt tcagcagaag
     2881 atgaagtagt catttctgat gatgggtcag tagatgatac atgggatata ttaactgaat
     2941 acgcagaaaa agataagcga atccagcttt tttcggggcc tcgtcaagga ttgatcgcta
     3001 attttggtta tgcgatccag cagacgaaag gagatctaat ctttttggca gaccaagatg
     3061 atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta ttttgagcag aatccggatc
     3121 agctagtagt agtttctgat ttaatcattg tggacagcga gctgcaagag atccatgatt
     3181 cgtatttttc gtttcgaaat tccaaagaag gatggcttaa taatgtgata agaagtcact
     3241 atattggtgc tggtatggct ttccggtctt ctttaaaacc cgatattttg ccgatcccgt
     3301 cagaagtgcc gatgcatgat atgtggattg gtttgctggg aggaaagaag actgggttcc
     3361 taagagaacc gttaactctg taccgaagac atgatttaaa cgcgagtgag attgaaacac
     3421 attctaaatt acggcaaaaa atttcttggc gtgttcatct gattacggcg ttgatcaaac
     3481 gaaaatggtt taaacgttga taaaagatgt ttaaatcctt aatatttcca tgacaaatat
     3541 aacaattgat gagataataa caaagtgaaa tttgaaatca aaaaggagag cagatatcca
     3601 tgaagggaat cattcttgct gggggcagcg gaacacgtct gtacccttta acaaaggcaa
     3661 catcgaaaca attgatgccg atttatgaca aaccaatgat ttattatcca atgtctactc
     3721 tgatgttggc gggaatcaaa gaaattctga ttatttcaac gccacaagat acaccacgtt
     3781 tcaaggaatt atttggaaac ggaaatgatt taggactgca gatcgaatat gcagtacaag
     3841 aaagtccaga tggattggca caagcgttta tcattggaga agaatttatt ggagatgata
     3901 gtgtctgctt ggtattaggg gacaatattt actatggtgg cggtctgtct aaaatgttgc
     3961 aacgagcagc ttcaaaagat acaggcgcga cagtatttgg ctatcatgtc aatgatccag
     4021 aacgctttgg tgtagtagaa tttgatgaga atatgcaagc tttgtcgatt gaagaaaagc
     4081 ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta tttttatgac aatgaggtaa
     4141 tttctatcgc taaaggtatc aaaccttcag aacgtggaga actagagatt acggatgtca
     4201 ataaagcata tttggataaa ggaaaactat ccgtagaact tatgggacgc ggatttgctt
     4261 ggcttgatac aggaacacat gaatcattgc ttgaagcatc tacctttatc gaaacaattg
     4321 aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat tgcttaccgt atgggctaca
     4381 tcgataagga acaattgctg gcccttgcac agccattgaa aaaaaatcaa tacggtcaat
     4441 atttattgaa tttggccgct gaataaggag tccttacaga tgaaagtaac agatacaaaa
     4501 ttaacagatg tcaaaatcgt agaaatggac gtctttggcg atcatcgcgg tttcttcaca
     4561 gaaagttatt caaaagcaaa atttgcagaa catggattga aatatgactt tgttcaagac
     4621 aaccattcac tatcagctga agctggtgtc cttcgggggc ttcatttcca aaaaggagaa
     4681 gctgcacaga caaaattgat tcgggtagtc actggagcag ttttagatgt agtagtggat
     4741 atccgcaaag gtagcccgac ttatggtcaa tgggacggat ttatcttatc tgaacataat
     4801 catcgacagt tgcttgtacc taaaggatat gcccatggat tcgttacttt gacaccaaat
     4861 gtgaacttcc tttataagtg tgataattac tataatgcag aagcagatgg gggcatcgca
     4921 tttgatgatc cagatttagc gattgattgg ccaattgatc catcaagagc gattatgtcg
     4981 gaaaaagatc accgtcatcc gacattaaaa gaatttgaag cagaaaatcc atttgtttac
     5041 ggagaaatct aagaattaag gagaaatttt cttatgaaaa acatcattgt taccggaggt
     5101 gccggattca tcggctctaa tttcgttcat tacgtggtaa acaatcatcc agaagtgcat
     5161 gtcactgttt tggataaact gacttatgca ggaaacaaag agaacttggc cggacttcct
     5221 agcgatcgcg tagaattggt cgtaggagat gttgcagatg cagaattagt ggaccgtcta
     5281 gtaaaagaag cggacgcagt tgttcattac gcggcagaat cccataacga taattcatta
     5341 aaagatcctt tcccatttgt acaaacgaat atcattggta cgtatacact tttagaagct
     5401 tgccgcaagt atgatgtacg ctatcatcac gtatctactg acgaggttta cggcgatctt
     5461 ccattacgtg aagacctgcc aggacatggg gaaggcgaag gagaaaaatt cactgcggaa
     5521 acaccatata atccttcaag tccatattct tctacaaaag ctggttcaga tctgttagta
     5581 aaagcatggg tgcgttcttt tggcttacgt gcaaccattt caaactgttc aaacaactat
     5641 ggtccttatc agcatattga aaaatttatt cctcgccaag taacgagtat cttaagcggt
     5701 attcgtccaa aattatatgg cgaaggaaag aatgttcgtg actggatcca tacgaatgat
     5761 cattcttcag cagtgtggct gattttgacg aaaggcagaa ttggcgaaac ctatctgatc
     5821 ggtgcagacg gtgaagaaga taataagacc gtgatggaat tgatcttaga aatgatgggc
     5881 caaccaaaag acgcatacga ccatgtaaac gaccgcgcag ggcacgattt gcgttatgca
     5941 atcgattcga caaaattgcg agaagagcta ggttggcagc ctgagtttac gaacttccgt
     6001 gatgggttgg ctgacacgat caaatggtac gaagaacatg aagactggtg gaaaaaagaa
     6061 aaagaggccg ttgaagcagc atatgccaaa aatggacaat aatacagagg aaatttcact
     6121 gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg tctctttttt aaaataaaaa
     6181 tcaaggagcg gataaccata tgtttttatt aactggagga aatggacaat taggtacaga
     6241 attgcgccat ttattagatg aaaaaaatct gccatatgtt tcaacagatg caaaagactt
     6301 ggatatcaca gatgctgaga aaacaatggc ttatattaca gaattaaaac ccgatgttat
     6361 tttccattgc gcagcttaca cagcagtaga taaagcagaa gaagaagcaa aagaattaga
     6421 tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt gcagcaaaag ctgtgaatgc
     6481 acaattcgtc tatatcagta cagactatgt tttcgatgga aaaaacaaaa atgtagaata
     6541 cgaagtagac gaccaaacga atccactaaa tgaatatggt cgtacgaaat tattaggcga
     6601 aaaagcagta gaagagattt tagaagacta ttatatcata cgaacttctt gggttttcgg
     6661 gatctatgga cacaatttcg tcttcacgat gcaacgccta gcggaaaccc gcgacaaact
     6721 gactgtagta aatgaccagt ttgggcgacc aacttggaca cgtactttgg cagaatttat
     6781 ggtatatgtc atcgaagaaa aagcaccatt tggtatttat catttgtcga acgaaaatag
     6841 ctgttcttgg tatgagttcg caaaagaaat attgaaagat acagatgtag aagttgcacc
     6901 tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg cagtattcag tcatgagcct
     6961 gaaaaagaca gaagcgttag gttttgtcat accaacttgg caagaagcac ttgcgcaaat
     7021 gctggaagct gccaaaaaaa tgaataagta atttcctttt tatcaaatag aatttggagc
     7081 atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa gcgaatcagg
     7141 cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt ggcgttttag
     7201 tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt tgggataaat
     7261 atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag aatcgggaag
     7321 tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct gaagaggact
     7381 tttcgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt gcgtataatt
     7441 ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc tttttcctcg
     7501 gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg
     7561 gattcgaagt actgatgata ttaacgaaga ggaaaaaagc tctatcgcta atcggtgcat
     7621 ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg ggcgatttga
     7681 ttttctttac tttgggactg atgattgctt tttatcatta tttctacaat catgataaaa
     7741 aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg tttattttag
     7801 tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat tttgttggcc
     7861 tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg ttgattggag
     7921 gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca aaagacgcat
     7981 tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga ggaaagtgga
     8041 cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa gatatcaact
     8101 attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta ttcctagcaa
     8161 gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga cgtattttga
     8221 tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa tggctagcag
     8281 aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt agttttgcgg
     8341 cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt attcctattt
     8401 ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg attacgcatt
     8461 cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg ttgggaacag
     8521 tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct tttgtcgcct
     8581 tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg
     8641 aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt aaagtttggt
     8701 tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc ttcaatacag
     8761 tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa gaatacgaga
     8821 agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat acaaagttca
     8881 ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca gaaaaattag
     8941 ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta tatgaagcgc
     9001 agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa gtagaatatc
     9061 cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat
     9121 aggctagctg agaataatcc tgaaatatta gtagaaagga gagtgaagat aattcaagca
     9181 atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt gctgacgcta
     9241 gtcttttttg tttggacagc aggggtaggt tttccagcaa ctttgtttat atataacaat
     9301 agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt ttataaagca
     9361 aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt cttttcttac
     9421 tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt ggttatttta
     9481 ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat ttatctgatt
     9541 gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat ccaagtacca
     9601 gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat caatacgaat
     9661 acaatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc cagttctttt
     9721 gaacgatggt atgttcgaat catcggtggg atagcagtcg ttgcagcctt tttaggtatt
     9781 tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat attggatctt
     9841 ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcctacac ggttacgatt
     9901 cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc
     9961 gatcgagaag atatttggca taaattctat cagacgctag gagaaaaatc tgaacaagtt
    10021 ttactaggaa tgaaaacatt tatcttccag cgaggaaacc aatttttagg aaaccacaat
    10081 agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc tgctttgctg
    10141 cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg gcagatgact
    10201 tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac attgacttca
    10261 tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg atatgatgaa
    10321 ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat gacttatgat
    10381 gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca acgctatcat
    10441 tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt tggtttattg
    10501 caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta tcatagaaaa
    10561 ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg cacttgactg
    10621 taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat ttatttatga
    10681 aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac ttcaaatcaa
    10741 gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta tttactgtat
    10801 tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc
    10861 catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca gatgcatttg
    10921 catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc gtattcaata
    10981 ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccattta ttcttcattc
    11041 tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg tctttacagc
    11101 tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg ctgacggcat
    11161 cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg cagttgatca
    11221 tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt gtaagcatat
    11281 tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt ttaagtgaag
    11341 cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg ttagtaatgt
    11401 tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat gtgctgtaag
    11461 gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat cttataatat
    11521 gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga aatcctatca
    11581 tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg
    11641 tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta cgggagtact
    11701 gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc tagaactagg
    11761 atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg gaatggttct
    11821 aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat ttgctgacat
    11881 cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg ttcgtctagc
    11941 ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag cattggcagt
    12001 aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca agaactcggg
    12061 aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt gtgatcgttc
    12121 ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag taacgacgaa
    12181 ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata aaaaccaagt
    12241 agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt tggataaaga
    12301 caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag taacttatga
    12361 agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa cagtcgacaa
    12421 tttttatgta tgcggactta atactctact agatggtatc aagattcctt ggaaaaaagg
    12481 agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg aatattattt
    12541 tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga caaagtacat
    12601 gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag accataaatg
    12661 ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat gaacaaagca
    12721 gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag tttggtcccg
    12781 gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg agcattgttg
    12841 agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga ttttatggtg
    12901 atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat tacgtcttgt
    12961 ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa gtcagagaat
    13021 ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat aattctgtca
    13081 tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta gatgaaaccc
    13141 ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg ggattattca
    13201 ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa tcaacttatg
    13261 aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt gaagtctacc
    13321 aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca gagccacaaa
    13381 attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat gaaacaatcc
    13441 aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc cccgtatcag
    13501 aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc gaattcagcg
    13561 cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc aaaacagtaa
    13621 atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg gatatttctg
    13681 ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca ccaacaatga
    13741 aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt tctgagaaaa
    13801 atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga ctagaccata
    13861 cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa tggacaataa
    13921 aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa aaagaacttt
    13981 ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa atcatcggat
    14041 ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct gaccaatcgc
    14101 catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa cagctcgcaa
    14161 aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt tcattcttag
    14221 tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata atgattaact
    14281 tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca
    14341 aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt
    14401 gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg aagcaacaca
    14461 tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc aaagcggttt
    14521 caaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc aaacagcgag
    14581 acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg acggcattga
    14641 atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg caaggactca
    14701 tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat cgatcgacta
    14761 tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga aagaagcaca
    14821 aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg ttgaaatcaa
    14881 atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt gggaactatg
    14941 tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat cctatcaagc
    15001 aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca gtctggcaac
    15061 taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata atgatgatga
    15121 attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc cagaactgga
    15181 tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg atccgcattt
    15241 caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc acttaggcgt
    15301 atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg aaggttctca
    15361 agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata tttaccacat
    15421 tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta atggcgaagc
    15481 caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt ctagaagaaa
    15541 catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg cttatgacgt
    15601 cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg aagatctaaa
    15661 aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa tcattattgc
    15721 ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca agcatcagct
    15781 gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc gaattaacaa
    15841 tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg atacagaagt
    15901 gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc gaatcggctg
    15961 tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag tattattagg
    16021 aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt gtggttattt
    16081 cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta tgatggtcaa
    16141 ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag cattcaacga
    16201 tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg cacatcaagc
    16261 agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag aaaaacaaag
    16321 acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata ttgcgcatga
    16381 tccattttac aatgataatt taacaaaaga cggaatcggc gacttttcgt tgagaccaga
    16441 ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg ctttttatag
    16501 ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag gttgggacag
    16561 aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg
    16621 aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg tttttaaaga
    16681 atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca gagcgccaat
    16741 agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc gatattttat
    16801 tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa agccactcta
    16861 tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt cttagcgtca
    16921 atataaagaa catttccact attaccaatt gctgaatata tttcgttctc agaatctaat
    16981 acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact ggttctaggt
    17041 ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata catcccttta
    17101 tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa accatatcct
    17161 gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga cttatccatt
    17221 gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt tggtatccct
    17281 ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact tgtctgatac
    17341 aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc ggttatacct
    17401 tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga acagactttt
    17461 gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg cttgattgta
    17521 atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata atctaatact
    17581 ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag caaagataca
    17641 atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc attccattca
    17701 tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt tggttgtcga
    17761 aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac tttcttctat
    17821 aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta atacaggtat
    17881 tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata aaggttcact
    17941 ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc aacattagtg
    18001 aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag aggtgtataa
    18061 agctttttat tagacatttt taaattccat tacaatttgc ttataaacta ggtttttaaa
    18121 ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat atatgaataa
    18181 aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg ttattgtata
    18241 caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc cattacgaaa
    18301 tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg cgttgaacat
    18361 tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt ttgtcacagt
    18421 catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg gccgctggtt
    18481 tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt tcttaagtat
    18541 ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa aggtcgtcat
    18601 tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa agaaaaacaa
    18661 caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca aaaatatgat
    18721 taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa aaatcaaaat
    18781 ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat ttgaaaactt
    18841 gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg aagcatctgg
    18901 atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca tcgtttcact
    18961 tgttttatta gttattgctt caccatttat gttattcaca gcaattttag tcaaagtgac
    19021 atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa aggaattttc
    19081 aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac cagttttagc
    19141 taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg tccgctttga
    19201 tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag gaccacgtcc
    19261 tgaacgttct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact acttacggca
    19321 taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg tctctgatta
    19381 caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga tgcttgattt
    19441 aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc aaggacttga
    19501 agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaa
//
LOCUS       DARBER010000039        18511 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARBER010000039 DARBER010000000
VERSION     DARBER010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.039"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(120..1886)
                     /locus_tag="SCK06_001334"
     CDS             complement(120..1886)
                     /locus_tag="SCK06_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151351.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1846..3600)
                     /locus_tag="SCK06_001335"
     CDS             complement(1846..3600)
                     /locus_tag="SCK06_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151352.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3710..4279)
                     /locus_tag="SCK06_001336"
     CDS             complement(3710..4279)
                     /locus_tag="SCK06_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG1151353.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4297..5712)
                     /locus_tag="SCK06_001337"
     CDS             complement(4297..5712)
                     /locus_tag="SCK06_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1151354.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5723..6391)
                     /locus_tag="SCK06_001338"
     CDS             complement(5723..6391)
                     /locus_tag="SCK06_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1151355.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6522..7106)
                     /locus_tag="SCK06_001339"
     CDS             complement(6522..7106)
                     /locus_tag="SCK06_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1151356.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7441..7671)
                     /locus_tag="SCK06_001340"
     CDS             complement(7441..7671)
                     /locus_tag="SCK06_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG1151357.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(7686..8633)
                     /locus_tag="SCK06_001341"
     CDS             complement(7686..8633)
                     /locus_tag="SCK06_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1151358.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8633..9475)
                     /locus_tag="SCK06_001342"
     CDS             complement(8633..9475)
                     /locus_tag="SCK06_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1151359.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(9608..10333)
                     /locus_tag="SCK06_001343"
     CDS             complement(9608..10333)
                     /locus_tag="SCK06_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1151360.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(10794..11108)
                     /locus_tag="SCK06_001344"
     CDS             complement(10794..11108)
                     /locus_tag="SCK06_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1151361.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(11124..12719)
                     /locus_tag="SCK06_001345"
     CDS             complement(11124..12719)
                     /locus_tag="SCK06_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HEG1151362.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(12742..13632)
                     /gene="iolE"
                     /locus_tag="SCK06_001346"
     CDS             complement(12742..13632)
                     /gene="iolE"
                     /locus_tag="SCK06_001346"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEG1151363.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(13649..14674)
                     /gene="iolG"
                     /locus_tag="SCK06_001347"
     CDS             complement(13649..14674)
                     /gene="iolG"
                     /locus_tag="SCK06_001347"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEG1151364.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(14698..15705)
                     /locus_tag="SCK06_001348"
     CDS             complement(14698..15705)
                     /locus_tag="SCK06_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1151365.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(15818..16978)
                     /locus_tag="SCK06_001349"
     CDS             complement(15818..16978)
                     /locus_tag="SCK06_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEG1151366.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(17054..18508)
                     /locus_tag="SCK06_001350"
     CDS             complement(17054..18508)
                     /locus_tag="SCK06_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEG1151367.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         5710 a         3853 c         2881 g         6067 t
ORIGIN      
        1 tcaatcatac tgattctttc gtgtattcaa aattagatgc tgtttaataa aattattctt
       61 tttgaaaaag aattataact atatcccaaa aattgtaggt taatttaaag cctatttcat
      121 catcgtgatt gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg
      181 agatttttgt atatttctga ccttcgatac aactcactat gagtaccttg atcaactatt
      241 tttccttgat ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat
      301 ataattagta ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca
      361 ttttccgcat ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa
      421 aaagcccgtg ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct
      481 ccgaccaatg tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa
      541 cgagctgctt ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc
      601 aaaatacttg tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa
      661 gaagctggag atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa
      721 tcatacagtc tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact
      781 aacgcagtca cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg
      841 ttatcatatg agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct
      901 gatccctctt ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa
      961 taaaatattt caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg
     1021 gctagtaagt aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca
     1081 cctactagaa tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact
     1141 gaaaaagtac ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa
     1201 agtctgtctt gaactttttt agatagatta aagctattaa tctcttgctg caattctata
     1261 gtttcttgaa attcttccga gttttctctc aaagtatcgt aatatttagt attggctttc
     1321 gtttgggatt tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca
     1381 gctaatccca ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga
     1441 gccataccgc cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca
     1501 gacatgatag tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa
     1561 tttgacaaat cgtctgcaat ttgcattctt aaatgcgcac tttctttata agtactatta
     1621 tataagtttt catactccct atctaacaac caatacatga aaagcaaagt aacgaacgat
     1681 actgtaaaat aaagccaagg cggctttaca ttttctaaaa ctaattcgtc tatcagcatc
     1741 attataatga atgctggtac catattaatg cagtatacaa aaaagctaga aatagatgct
     1801 ttaaccaaat cttctgcgcc ctgattagtt aaagcgaatt tgtttttata aaaacttttc
     1861 attgtcaagc ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg
     1921 atgtttagcc attaattcat tatgatttcc acgttctact attttccctt ctgacaagac
     1981 aagaatttca tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt
     2041 ttctttcatc agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga
     2101 tgcactggct tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc
     2161 tatacgttgt ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc
     2221 tctttcggga aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac
     2281 tttacttgct ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa
     2341 tagtttcgta tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg
     2401 atttaaaggc ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat
     2461 tagtttagct atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt
     2521 ttgttctaaa actaagttga aattttctaa aacacactct tctttgtatg aaaagctgac
     2581 attttcaaat gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt ttagttggtc
     2641 ttgcttcatt tctaaataaa gggagtctag tgtttcgact gcatatcttg cttcaaacaa
     2701 gtgcatactt acatacatta ttctcatgaa agaaacaaaa ataattccac ttaaaaaaaa
     2761 caacatcaac aagtcaacag ctaatatctt gggagtggta agagaagtta taaaaaaggt
     2821 tataggaact atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa gaacaaaagg
     2881 tgttttccca ctcaaagaat acttatatgc aaacttagca taatcgttaa ttgctttatt
     2941 caaggcttta aatgatgtta ctttagcacc aaatatcttt ataacttgaa ttcctcggat
     3001 atattctacc gtttcagagc ttaacacatc taacgattct tgatattttt gaataaaatt
     3061 tgtattctct gtcattttct ttaacaagaa tacaccaatt actaacatgg cgattagaac
     3121 caatccaatt ttaaaattaa tgaaaaagcc taaggcaact gataaaacag gtgttactat
     3181 ggcttgagca ctatctggaa tcaagtgagc aattgccata tgtgttttag ccgcgttgtc
     3241 atcaattgtc tttcgaatat atcccgaaga atttaaatca aaaaaacgaa aacttgcttc
     3301 tgttaaaccg ctaattccct tttttctcaa attagtctct aagcgaaacc caaggatatg
     3361 agaaaataag ccagaaaaaa tataaaataa agctcctaaa gttaaccaag caacagtttg
     3421 aaccgcataa ctcttagcta aatattcatc tcctacaata attagagact taagaaattt
     3481 gtaaatactg taatatccat agactgttaa tacgacagaa aaatcagaaa ataaagtggc
     3541 taaccatccg tagtatttca ttttagggac ataactaaat aattttttgt atactttcat
     3601 tttttcctcc cccgcattca atttcttctc ttattcgttt acaactgtta atgctttccg
     3661 taatagcgta aattagagat gtacaaacag atagaaggta aactacgttc tattttttgt
     3721 atttttttaa gacttttctt ccaatcaata caccaatcat agataaactt gctacaacta
     3781 acactgaaat gattccccat atacctaccg catagtggat cattgcatcc attgttgcag
     3841 cagattgtcc tttatctatc caatcttgac gaaaacgatc tgtaaagaac catataggaa
     3901 tccactgtcc ggcagcactg cctaattgca ttagagccca agacgcaact tgaggaattg
     3961 ctcgatcata tccaaataca gatagaataa tatcagctaa gatcgccata ctaatcgaac
     4021 tgataaacca aggtaagtac gccccgccca ttaacgtaaa aaataacaat aaaacaattg
     4081 tgtaaatagt aaaaattcct tttctagggt agttatagct aatataaaca aatatgattc
     4141 cagataataa tccaaaaatc cctggggata tgctgtgacc aaacgcccca ccaatactac
     4201 taaccattat tgaaaaaaag taaattacta gggaaataac tgtagctaaa ccaataaaaa
     4261 tataatcttt tgttttcata tatattctcc tcaattctat ttcgttatgt cattaaaaat
     4321 ctgttctatc aaagttgaat cttctactct aagttttata tcttttttta ttccaccatt
     4381 ttttaaataa ataattctat tgcatgtttt ataaagaaat tccggatcat gagtggctat
     4441 aactactgca cccttttctt ttaagtcttt aataaggtca gcaatccttt ccatattaat
     4501 ataatccaac ccacttgtag gttcatcaaa tagaaataaa ttagtttttg acaatgaagc
     4561 tatagaaacc aataatcttt gtttttctcc accggataaa tcaaacggta attgatcttt
     4621 cattttataa agttccattt tttttaaaat tttttcttgc tgatctttac taactatttc
     4681 attccccagg gctaactcgc tcctcactga ttctgtgaaa aattggtaat ccatttcttg
     4741 cattactaaa aacggcgatg acaaattttt tttattttcg attatctttc ctttggtagg
     4801 gcgaataatc ttcatgagta ttcttagtaa ggttgttttc cccacaccgt ttttccctac
     4861 caaaccaata acatctccaa ctttgacatc aaaatttatg tcttttagaa tattttcata
     4921 agtaatttct tcaactttta atacatctgc acttaccttt tcgatatagg gaacttcaat
     4981 cgaactatta aaaatgtcaa atttccttaa tgcgttattg ttttcagttt ttaacaactg
     5041 aatagttgta gtttttacag ttctattttt aacaaataga acttgatcaa ttaattcttt
     5101 caaatagtaa aaacgatgtt cagctataac aatagtaaat cctttatttt ttaaatcaca
     5161 aagtaagtca gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg gttcatccaa
     5221 tataattaat ttagagttta gcgataaact acatccaata gaaattttct gccgttcacc
     5281 acttgatagc gaaaaaatgt ttttatctaa tatatttgaa agatctaata attcagctaa
     5341 ttcattgatt cttttatcca tatgctcttt tgtaaatcca aagttttcca tcgaaaatac
     5401 taactcactc gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac ttacgtagcc
     5461 aatatttttt gatatacttt cttgtgttaa ggattgtagc gagttatttt caaataaaat
     5521 ttccccaacc aattttccgt cataaagtct tggaataagt ccattcatta actttaatag
     5581 tgtggatttc ccactcccag aagaaccagt aatcaatgta agactacctt tttttattga
     5641 aaaattaagg tcaacaagcg cttgttcctt aacatcctta tatgaaaaag aaacattgtt
     5701 aaattcaaac aattctttca cctcaaatca aaatacttaa gaaaaaaata gtaacggaaa
     5761 acaggatgat atagtctaat aagctcatgt ttgttttata gtaagaactt cttttcacag
     5821 gagattctat cccctttgtt attgcattag cggtcatttc ttcagcaatc atagttgcac
     5881 gtataagaac gggaactaac agaaattcca aatattgcag aggctgaaaa aaagacaagc
     5941 gaatgtttct catttttaaa gattctttaa tcattcttat ttcagaacga taagtcggaa
     6001 agaatctgat cactactgta atacttaaca ttattttttt cggtaaatgt attttttcta
     6061 aagaggataa taattcattg ctagtataga cactaaccaa caaccatgag atcattacga
     6121 ccggcaacat tcgaaataca atatataagg atgtcccaaa aaatatagta aaaaactcta
     6181 tcttgatagc atcaataatt ttcattacta aaaacagaac tgaataaatc aagatgaaat
     6241 aaatacatgt ctttatttta cgaaacatca atactaaaac tgtagcaata atgaaacacc
     6301 ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag
     6361 taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac
     6421 tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa
     6481 agcataactg agaacaattc tcaatgcaag ctttttttta attaactcag taattcaatc
     6541 cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt ttcttttgaa
     6601 tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta gaagtggatt
     6661 tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa cttatctaga
     6721 caacagactg tcttttttgt cacaaatgat gtaatttttt caacaatatc ttgatatttg
     6781 gttccagctg attggaataa aaccaaatcg aatatacgtt tattctcaaa aatataatta
     6841 ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc tatattttct
     6901 aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc attaatatgc
     6961 ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata aaaagcaccc
     7021 gtagttacgc ctgcggtcaa gcacagttgc cttatcttag tttgctgaaa tcctactttt
     7081 tcaaaatggc ttagtccact ttctattaat ttttggtgag ttgtaatata atctctggtt
     7141 tctatgggta cttcctccta ataagatgaa ttaactatat tatcttacac aggacttgaa
     7201 gtttcaatcg ttaacgtcta ttactgaact cattccttag agctaaacta atgagaaaga
     7261 gcaaaataac tgatacaaaa agcaatccta caagaattaa tagactcatc taaatcattc
     7321 accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta ttaggataag
     7381 tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa agccttttac
     7441 ttaaaacctt tttttgtaat cgtcaataaa ttttttccct ttatcagtta attgaaatcc
     7501 tatatctaaa tagtcctcat atctaagttt agtcagcatc tcacttacag atgctggact
     7561 aacacttaga acttttgcta aatcattatt ttttaatttc ttttttcttt ttgataattc
     7621 ctgaattgct agtaaataaa ttagtatagt agttgtttta atttgtacca tacctaccct
     7681 ccaaattatt tcgaaaggcc ttcagcaatt ttatccaggt tccatttcat catcgcatag
     7741 taactatcac catcttgtcc tttttctgca attgaatcag taaaaatcgt tgaatagata
     7801 ggaatattgg tatcttttga tactgttttc attggtctat cgtccacact gctttctacg
     7861 aataaggagg gaacttttgt tgtgcgtaat ttttcaacta agtgttttat ttgatctggt
     7921 gttccttctt cttcagtgtt gatttcccaa atgtaagcag aaggcacatt atacgctttc
     7981 gagaaatatt taaagcatcc ttcacttgta acaatcattt ttttatcatt cggaattgaa
     8041 gcaaatttag atttagcttc tttgtctaga gaatccaatt tttcaatata cttatctaga
     8101 ttttctttat agaatttttt attatcagga tctttttccg ctaactgttt ttcaatattt
     8161 ttagcgtaaa taataccgtt ttctaaattt aaccaagcat ggggatcttc cttccctttc
     8221 tcactctgac cctctaagta aataacatct atgccatcac ttgctgcaaa atagtctttg
     8281 ttttcctctt tgttcgcatt ttttactaat tttgtaaacc aagcatttcc tccagtttcc
     8341 aagttaacac cgttataaaa aatcaaatct gcttttgaag ttttttgaac atcttcaggc
     8401 aaaggttcat attcgtgggg atctttccca ataggtacga tactgtgtaa atcaatttta
     8461 tcttttgcta tattttcagt aatatctgct aaaatcgaat tagtaactac tacctttaat
     8521 ttttcgttcg aagccactgt atctttgtct ttactatttg ttgaacatgc cccgaatata
     8581 aaaagagcaa tcaaaatccc cagacttccc agtactgctt tccattttct catcataact
     8641 ttctctcctt ctttcttaaa aatttttgtt ttggtgagat tagaaaacta attacaaata
     8701 agaatgccgc tgttaaaaca atacttgatc ctgctgctat gttaaaacta taaccaatga
     8761 acaaacctag caatgatgag cacgctccga ctgtcgccga aataaatatc atagttttta
     8821 aactgtttgc atataaatat gctgtagcag ctggtgtaat taacattgca acaattaata
     8881 tagttcccac actttgcata gctgttactg ctactaaagt taatagaatc attaatagat
     8941 aatgataaaa actgacattc atccccattg cttttgccat taatggatca aaagaagtga
     9001 ttaataattc tttaaaaaat agaacaactg taaccaacac taaaacagaa acgccaattg
     9061 taatccactt atcaatatct tgtacagcta aaatatttcc aaataagata tgaaataaat
     9121 ccgttgagct atttgcaact cctataagga taactcctaa tgctaaaaaa gaactgaaag
     9181 taataccaat tgctgtatct ccttttatta cactattgct ttttataaaa gtaataatga
     9241 tagaagctaa tagaccaaat acaatcgcac caataaaaaa attaatacct aaaatataag
     9301 aaattgctac tcctggcaaa acagcatgag aaatagcatc acccataaga gacattcctc
     9361 ttaaaataat aaaacaacct actgttccag aaacaatccc tataactaca gatgtaatta
     9421 gagcattctg taaaaagtga aagtcataca gaccattaat aaaattttga agcatattat
     9481 tccccttgta tattagagtt agggttttaa ccttccggtt ttgattggat aaccctatga
     9541 accatcgtta ttttaatttg gtattaactc atttcttttg agataatata tttattggat
     9601 tgagaggcta agccgttccc ccctctccaa taaatatagt atctccatag gcttttttta
     9661 aattctcttc attaaataca gattctacac ttccgtgagc aattagattt cgattcaaaa
     9721 gaataatatt atcaaaatac ttctttacct tgcttaaatc atgatgcaca attagcactg
     9781 tttttccctg atgtttcaat tcttttaacg tatccataat tattcgttca ctaactaagt
     9841 ctatccctac aaatggttca tctaaaaata tgaaatccgc attttgagca agacatcttg
     9901 ctaaaagtac acgttgaaat tgtcctccag ataattctcc tatttgatga ttagaatatt
     9961 ttcccatatc gaccttttcc aaagcctttg aaactctttg ccattctgca tttttaattc
    10021 tttgaaaaac tttcattccg gcatatgttc ccatagatac acattctttt acttttattg
    10081 gaaaagtaaa atcaatatca gctttttgct ctacatatgc aatcttcacc agttttttat
    10141 tgatatcttc attattaatg gagacctctc cctgatgagg gataatattt aacattgctt
    10201 taagcaatgt agattttcca gcaccatttg gaccaataat acctgttaaa gcaccttctt
    10261 gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt
    10321 tcacttctaa cataactttc tccttctaaa tgataatgat tatcacgatc atatggtata
    10381 caaaaattat attttagtca accctaaatt ttataattta gatttttttc tgttgttgaa
    10441 gattaggatg tcaaaattat ttgaaactac tcgtatagta aatagtaagt aattttcaaa
    10501 acacgaagaa aaacaaaaag aaaaaaggaa acgccgaagc atttcctttc agtgatttaa
    10561 tgagaacaac acgatttgac ttagagtctc aaaaagtcga ataggtacac tcatttaagt
    10621 gtctccattc gactttttgg ggatgagctg gctattgagg tccgcttacc ctattgaata
    10681 aatacaagat tgggtatttc taataagtcc gcccttcata ttggggtgat cggggcgaac
    10741 atgccatctt ttcatatacc acctcctcga cactcaataa tcaaagattc tcgttaatct
    10801 tcttcgatgc ttgcaatgat gttttcaata ttcatataac gaaaatcttc ttgtttgata
    10861 taaacgatct gagcggtaca tttttccttt ttgatgattt cttttacttt ttcctcagaa
    10921 cgtgcgacct gcggcgttaa aagtaaaacg tcatagtctt tataattcag tttttcttca
    10981 acgtttcgga tcgttgcata atctatctca tacgatagat ttttggctct gatcgtttca
    11041 agcatttttg atacgagcag cgacgcactg attccggtct cgcaaataat caatagatcc
    11101 ttatgcataa taccctcctg tttttagcct gcaaagccaa ttggtgaaaa tgccacataa
    11161 gttaaaataa caatgactac caatgctata gcagtcacct tggtgtattt ccaaggctcg
    11221 atatcgacct ttgtatcgaa tgagttcaac acaaattctc catcaggttt caatttggaa
    11281 accaccatca aaatgataat gtccagaacg aataatgccg tcaaaacata aaggaaatgg
    11341 atattttgga aaagcatttt tgatagtgca tacaagacga tatgcacggc aatcgtcgtt
    11401 tttgccccaa aagcagttgc tttttttgaa taaaaggcaa agagaatgat cacgagcagc
    11461 ggcatgttgt atagtccatt gaattcttga acgaactgat acaatccagc cggtgcaaaa
    11521 gaaatgaatg gcgctacgat aatggagata atccccacaa cgaccgttac tgttttacca
    11581 gcacgagcga tttgcttatc atttgctttc ttattgataa gcggtttata aaaatccagt
    11641 gaaaacaagg tcgatgtcgc gttcaaagct cccgcaaatg atgataagat cgcgccaaag
    11701 ataacagcgg cgaaaatacc aaagatcatt ttaggcaata cttcactgac taaaaaagga
    11761 tacgcgttat ctggatttga cattaaacta tcgccaaaaa tattgcgagc gacgatccct
    11821 ggaaagacaa ggaataacgc acctaagatt ttgaagacgc cgactaggaa agcgcccttt
    11881 tgtgcctcag ctaaattctt gccggacaat gctttttgga cgatcatttg attggtacac
    11941 cagaaataca gattgttaaa cagcatccct gtaaatagtg tcggccatgg aacaacagcc
    12001 gagtcgatcg atcccacaga gttaagcagc catggcgtgt ttaccacaac gtgttcgatc
    12061 ccgccaacaa agctgccgtc accaagcttc attaagccta aaattgggac cagcagacca
    12121 caagccagca gaccgactcc ataaatggag tcactattgg cacttaacga caagccgccg
    12181 atcagcagat agaggatgcc gacaatacca attgccatcg ctacaattat gatcgcgacg
    12241 atcggctgga cgcccagcag ctcatcaata tggaagatct tattgaacac cagcgatcca
    12301 gaataaagaa ccacgggtaa gaaagaagtc atataggtca caataaataa gatcgaaata
    12361 attcgtttgg taaccgtatc ataacgaatc tcgatgaaat ctgagattgt attgatgccg
    12421 tatttaaaat attttggcaa gaatatcaca gccaaagcta cgatcgcaat cgtagcagtt
    12481 acttcccagg ccattacttc catccctgca tgataacttt gaccattttg tccgactatt
    12541 tgttcggtgg acaggttcgt catgatgatc gtgccggcga ttggcaaagc agttaagctc
    12601 cgccctccca tgaagaaccc ttctgatcca gaagtatcga cacttctgga tctagaatag
    12661 gcaaataacc atacacagat gacaatcact aggaaactga ttatagaaaa aagattcatc
    12721 gtctagctcc ttttcaatag attaaagacc ggtgacctct cgaatgtagc ttctggcttt
    12781 gactgcatat tcaaatggat tggctttagc cgggtcctgt tctgcttcaa ctacgatcca
    12841 gccttcatag tcgctggcat ggattgctgt ccagattgga gtgaaatcaa tcatcccatc
    12901 accaggaacc gtgaagacgc ctttcttcac gccgtttaag aagctgtctt tttccttttc
    12961 tacttcttca gccgtttctt gacgaatatc tttgaaatgg atgtgtttga tacgatcttg
    13021 atgacggttg taaaccgcga ttggattttc accagaaaag accaaatgtc ccgtatcgaa
    13081 caataattta actttgtctg gatcagtcat ttccattaaa cgatcgattt cttccgtcgt
    13141 ttgaacgccg gtacccatgt gatgatggta aacgatctcc atatcctttt cgtgggccaa
    13201 atcccctaat ttttctaatc cttccgccag ttttttccac tcttcatcag tgaaaactgg
    13261 tttatcatca aagactggcg ctgtcataat cccttggata ctatgaccct gctcagctac
    13321 tacgatgact ttagcgccca tcgcatgtaa gaaatcccga tgtgctttga aggcttcggc
    13381 agtttcttca aaaggtttag ttgtcagaaa cgcactgaac catgcactgg caacctttaa
    13441 gccgcgaata ttcaaatatt gttccaacac ctctggatct tttggatatt tgttgccaat
    13501 ctctgttccg gtgaacccag caagggccat ttcactgata cattgttcaa aggtattttc
    13561 actaccaagc tcgggcatat catcattggt ccacgcgatt ggtgcgatgg ctaattgaat
    13621 ttgtttggtc atattctcac actcctgctt atttttttag ttgtactaaa ctcttcgtat
    13681 cgaatgattc ttgacaagcc aacgcgatct tagttgattg caatccatct tctgcattaa
    13741 ccgctggttg acgtttttct tgaatacatg agacaaattc ttgggcttca cgaatgaagg
    13801 cttctctgaa tcgttctggg aagttttgtg aacatggacg gatgatccct tgatcgttca
    13861 tgatcgtcac aagatttttc tcaggcgctt gcgctacccg cagcattcct ttggttccca
    13921 tgatctctgt ttcgacatga tagccatggg ctgcgtttct gccggcaact agtaaagcta
    13981 ttgttttatc gctcaggctc atcattgcgg ctccagtttc aagctcacca gcctgatcca
    14041 attctggata cgccgcattt ttcccaatcg cccacacttg atcgacttct tggccggtaa
    14101 accagcgaac caaatcaata tcatggatcg ccatatctaa gaacaagccg ccgctgtcgt
    14161 tattcatggc aaatttgaca aagctttcca agccttcgct cggatcgatc ccataacaac
    14221 gaatcagtgt gatttcgccg atttcgccat cttctaccat tttcttcgca tactgatacg
    14281 actcatcata gcggcgcatg aagcctaaca tgaagacctg ttcaggatgc tcttcgatga
    14341 ctttttgggt ctttttaata tcttcaactt caaggcctaa tggtttttca gtaaagacat
    14401 gcttgccatt ttccatcgcc aagcggactt ggtcacagtg aaagcctgat ggtgaaacaa
    14461 taaagactgc atcgatcacg gaacttttga ccatttcttc atagctggaa aaagtatgct
    14521 tgacgcctaa tttcttctgt gcgaatgcta attctgtttc aactaaactg cacgcagccg
    14581 taagctcaca acaactgaca cctgttgcta aattttgtgc atgtactttt cctaatcttc
    14641 ctaaaccgac gatgcctact ctaatgttgt tcatgaaata cctccgtttt ctgagaatta
    14701 ctcattgtat agtgctggtc gttcatccat ttgaatcatt acttttgttt ttgtttcacg
    14761 agactcatga caagccatca tggtgatcgc tgctacatac ccatcccaag cggtaggacc
    14821 gacaaccgca tcatttttga tcgaatcgat ccacagcttg aattcataat cataggcatc
    14881 caaaaagcgg gtctgccaat ctggtgaaac aggtgtgtaa tttttttctt ccgatttaat
    14941 tcgtgtatag gctgggtccg tcattcctac ttctgccgtt tcgaaaacca catcacattt
    15001 aatatcgtag ccaaagcggc aattcacaaa gatttccaaa tcaatcgtta caccggattt
    15061 tgtttgaaag atcagcagct gcggatcatg caaatcggca tgggagttgc tggttttttt
    15121 cggcaagatc atttgaacag attcataatc ttcatttagc aaccagcgaa gcacatcaat
    15181 ctcatgaatc gcggtgttgc tgatcgccat cggtgttgtg tagttttcat ctgcgctagg
    15241 atttctatgg gcacaatgca tcattaatgc ttctccgtat tgcttcgatt cgattgcttc
    15301 tttcagttca atgtagccgc ggtcatagcg gcgcatgaag ccaacctgaa ccaatttctt
    15361 cccataagca acttccgcat caacgatccg gcggcagcct gctgcatcca tcgctaatgg
    15421 cttttcgcag aaaacatatt tcccatgctt gattgcttct aacacatact gctcatgggt
    15481 cgggtcccaa gaagtcacaa caacgacatc cacatcttca gaagcgatca atgcttctcc
    15541 ggtttcaaag aatttagcat cgacacgttt ggcaacctct ttagcagctt ttacattgat
    15601 atctgagact gctgtcacat aagccccttg tgttttctga ttgatcctgt cgatatgctc
    15661 gatgccgatt gcacctgttc cgataacgcc tacttttact ctcaattact tatccccctg
    15721 ttctgttgtt taagcacaca tagtatgcgc ttacaaaatt attcctgtgt agcttttatt
    15781 cttaaaaggt agagccctct ctttgctccg ccttttacta cccataagct tgtacgtata
    15841 attccttaat ttgactatga ctaggcacct ttttattatt ttgcggactt ccgctggtca
    15901 acgcatccgc ggccattttg tctaaggctg ccataaacgc ttgctgatca ataccataat
    15961 ctcgaatcgt tgggatcgac agctgctcac agagctcttg gattttttta atcagataat
    16021 ccgcagcctc ttccagcgat aaatcttcgg ctgccggata aataaatctg gcgatcgtcg
    16081 ctaactcagt ctgaatagca tcctttgaga attcaaaaaa gaccggcaat aacatcgcat
    16141 ttgatatccc atgaggaatg tgaaaaagtg cccccaccgg tcgactcatt ccatgaatca
    16201 atgtgactga cgcattcgaa aaggcaatcc ccgcctccgt tgaagctaag ctcatttgtt
    16261 ctctggcttc agtgtcgtcg ctattttcat atgcctgaga aagcgtgttc aaaattttct
    16321 tcatcgcttc cagtgaaaat agttgcgtta cttcattggc tttgactgaa acataagatt
    16381 ccaaagcgtg acatagcgca tcgactcccg ttgccgcggt tacttttggt ggtacagttt
    16441 tcgtaaaatc agagtcaacg attgagatct gtggcatgaa aacgagatcc ttcatcatca
    16501 ttttgactgc tgtttttgta ttggtaataa ctgccacatc ggttacttcc gaaccagttc
    16561 cagcagttgt tgacactgca atcagcggaa tgcccgcttg cagctgatca aaatttcttg
    16621 catgaatcgc ttcgagactt aattgattag cagccaaaac ggctaccacc ttggctgtat
    16681 ccagacagct gccgccacca aaagcaatga tatgatcaca ttgatgcttc tgaaaaagct
    16741 tcagcgcatc ataaacattt tcttgcgtcg gttcggcttg aatgtctgta tactccgcta
    16801 attgatcatc tgtttttccc aacagctcct tgatcttttg aagatgcccg gtctgttcca
    16861 taaccgtatc actaatgatt aacggcttgt tgcctagttg ttctgattct tccataagaa
    16921 attggattgt tccgccgcca attcttaaat tggcaggcat tttcagttga atattcacag
    16981 aaaaaacctc tctctggctt atagcctata acagaatcct cgaagctctt taacagagct
    17041 tcgaagcaaa tctttaaatc tcactttgcg cataacgtgc tgtaacaact ttccgatggg
    17101 tataaaactc aacactgtct ttgccattgg catgcaaatc accaaagaaa gagtgcttcc
    17161 agcctgaaaa ggcaaaccaa gccatcggtg caggcacccc aagattgacc cccagcatcc
    17221 cagcatcaac attttctctg aagtagcgaa tcgctgatgc gttgttggta tacagacacg
    17281 aaccattcgc aaattcagat tcattcgtca gctcaacggc tgccatcaaa tcttttaccc
    17341 gcacgattga tgcgaccggg gcaaaaatct catcctgcca gatcttcata tccatcgtga
    17401 cattatcaaa aatcgttggt ccaacaaaat agcctttttc tggaatgttt tcgcgaccat
    17461 ctagtaccag tttcgctcct tcatcgaccc ctttttggat atagtcaaag gtccgctgct
    17521 gtgcatcttt tcgaatcact ggtcctaaaa agaccccttc gtccaagcca ttgccgatct
    17581 tgatcgcttt tgccgcagcc ttaaattttt caacaaactg atccgcgatt tcctcttcca
    17641 aaacaatgac ggaggctgcc atgcagcgtt caccagcaga accaaaagct ccgccaataa
    17701 ggtccttgac tgcccgatca atattggcat ctttcagcac aacaacatga tttttcgctc
    17761 cagtcaacgc ttgtacccgt tttagatttt ttgttccttc acggtaaacg tattcaccga
    17821 cattttttga tcctacaaag ctgatgcctt ttaccagtgg atcttgcagc aatgtgttga
    17881 caacatcgtg ggcaccatag acaacattaa aaacaccgct aggaaagcct gctttttcaa
    17941 ccagctcaac aattttctcc atcaacaatg gtgttttttc tgaaggcttc agcaccaccg
    18001 tatttcctgc cgccagagcc atcgggaaga tccagaaggg caccatcatt ggaaaattaa
    18061 acggtgtgat ccctccgatt actccgattg ggtaacgata attcgtgatc tcaatatcag
    18121 tcgccgctgt tgataaagaa tctcccatca ttaaattggt gattcccgct gcgtgctcca
    18181 cattttcgat tcctcgttga acttctccca gtgactcgga caatgatttc ccattttcca
    18241 gcgtgatcaa acgcgccaat tcacttttat gatctgttaa aagctgatgc agcttgaaca
    18301 gataacgtcc gcgaacagga acaggaactt ttttccaagt ttgaaacgca gcatcggcag
    18361 ctttaactgc ttcggctgtc tctgctttgc tagacaacgg tacattcgcc agcgcttctt
    18421 ctgtagccgg attcattact tcttcaaact ctgttgagcg actagcgacc caacgaccat
    18481 caatataatt tcttaaaaat cttgtcattt a
//
LOCUS       DARBER010000040        18300 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARBER010000040 DARBER010000000
VERSION     DARBER010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.040"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            408..965
                     /locus_tag="SCK06_001351"
                     /pseudo
     CDS             408..965
                     /locus_tag="SCK06_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1023..3062
                     /locus_tag="SCK06_001352"
     CDS             1023..3062
                     /locus_tag="SCK06_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG1151368.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3171..4322)
                     /locus_tag="SCK06_001353"
     CDS             complement(3171..4322)
                     /locus_tag="SCK06_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1151369.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4476..5006)
                     /locus_tag="SCK06_001354"
     CDS             complement(4476..5006)
                     /locus_tag="SCK06_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1151370.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5105..5455)
                     /locus_tag="SCK06_001355"
     CDS             complement(5105..5455)
                     /locus_tag="SCK06_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG1151371.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5612..7141)
                     /gene="ahpF"
                     /locus_tag="SCK06_001356"
     CDS             complement(5612..7141)
                     /gene="ahpF"
                     /locus_tag="SCK06_001356"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG1151372.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7151..7714)
                     /gene="ahpC"
                     /locus_tag="SCK06_001357"
     CDS             complement(7151..7714)
                     /gene="ahpC"
                     /locus_tag="SCK06_001357"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG1151373.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7858..8520)
                     /locus_tag="SCK06_001358"
     CDS             complement(7858..8520)
                     /locus_tag="SCK06_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1151374.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8704..10350
                     /locus_tag="SCK06_001359"
     CDS             8704..10350
                     /locus_tag="SCK06_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1151375.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10341..11423
                     /gene="uxuA"
                     /locus_tag="SCK06_001360"
     CDS             10341..11423
                     /gene="uxuA"
                     /locus_tag="SCK06_001360"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1151376.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11531..13084)
                     /locus_tag="SCK06_001361"
                     /pseudo
     CDS             complement(11531..13084)
                     /locus_tag="SCK06_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13232..14373
                     /locus_tag="SCK06_001362"
                     /pseudo
     CDS             13232..14373
                     /locus_tag="SCK06_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14499..15578)
                     /locus_tag="SCK06_001363"
     CDS             complement(14499..15578)
                     /locus_tag="SCK06_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1151377.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15687..16127)
                     /locus_tag="SCK06_001364"
     CDS             complement(15687..16127)
                     /locus_tag="SCK06_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1151378.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16184..16594)
                     /locus_tag="SCK06_001365"
     CDS             complement(16184..16594)
                     /locus_tag="SCK06_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1151379.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16775..17209)
                     /locus_tag="SCK06_001366"
     CDS             complement(16775..17209)
                     /locus_tag="SCK06_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1151380.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17309..18157)
                     /locus_tag="SCK06_001367"
     CDS             complement(17309..18157)
                     /locus_tag="SCK06_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1151381.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         6282 a         3386 c         3291 g         5341 t
ORIGIN      
        1 atattaaaga ttgttaaatt tcactatttc atttcaaatt ccattgccta atcattagga
       61 aaagtctatc atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta
      121 tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg
      181 ttggattaag gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa
      241 ctcggacgat gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc
      301 tttatatttt tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg
      361 attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat tggtggaggt cgctcggatt
      421 cacttctggt tgcacaatac aaccagaaaa cacaacaagc aaaactgatc tctatcatga
      481 gagattcata tgtcgatatt ccaggttacg gaatgaataa gataaatgca gcttactctt
      541 atgggggagt tgatttatta aatcaaacat taaaggaaaa tttcaaattt gaagctccct
      601 attatgcaag tatcacattt caagatttta tcgattgcgt taatgaactg tttcctggtg
      661 gagtcaagat cgatgcagaa aaatctctag atttagatgg cgtatatata aacaaaggag
      721 agcaagtgat ggatggtaat actttactcc agtatgctcg atttcgggaa gacaaagaag
      781 gagacttcgg gaggatcaga aggcagcagc aagtgatcaa agctatctct cagcagctga
      841 aagacgtaac ttcaatattc aagctaccca aagcggttgg gaaattactt ggtagtatcc
      901 aaacgaacct tccagaaagt gtgcttctgg attgtggtat ggatttcctc aaagacatat
      961 gttagaataa acaggtataa atagtgaaat aaaggaatga caagcaagag aaggaggaaa
     1021 aaatgaaaag aagtgacaag cacggcaaaa atcgaacagg cgcttatatt gccggcgcag
     1081 tgatcttaat agcagctgcg ggtggcggtt atttttacta ccagcactat caagaaaccc
     1141 aagcagtaga agctggagaa aagacagttg agcaatttgt ccaagctttg aacaagggag
     1201 attataacaa agctgcagaa atgacatcaa aaaaggcggc aaataaaagt gcattatctg
     1261 aaaaagagat cttagataaa taccagaata tatacggtgc tgctgatgtc aaaggacttc
     1321 agatatcgaa tctaaaagta gataaaaaag acgattctac ttacagtttt tcatataaag
     1381 caaagatgaa tacctcatta ggtgaattga aagatctttc ttataaagga acattagaca
     1441 gaaatgatgg tcaaaccacg atcaactggc aacccaactt ggtttttcca gaaatggaag
     1501 gaaatgacaa agtaagcctg accacacaag aagcagcaag agggaatatt atagatcgaa
     1561 atggtgaacc attagcaaca acaggcaaac taaaacaatt aggagtcgtt ccaagtaaac
     1621 ttggagatgg aggcgaaaaa acagccaata tcaaagcgat tgcttcctcc ttcgacttaa
     1681 cagaagatgc tatcaatcag gcgatttcac aaagctgggt acaacccgat tactttgtcc
     1741 cattgaaaat cattgatgga gcaacgccag aacttccagc tggagctacc attcaagaag
     1801 tagacggcag atattatcct ttgggtgaag cagctgctca gctgattggt tacgtgggag
     1861 atatcacagc agaagatatt gataagaatc cagaattaag cagtaatgga aaaatcggtc
     1921 gctcaggttt ggagatggct tttgataagg atcttcgtgg gacaacaggt gggaaattaa
     1981 gcattacaga tgcagacggt gtagagaaaa aagttctgat cgaacatgaa gttcaaaatg
     2041 gcaaagatat caaattgaca atcgatgcga aggcacaaaa aatagctttt gacagtctag
     2101 gaggaaaagc tgcatcaact gttgcgacaa cgccaaaaac cggtgatctt cttgcgcttg
     2161 ctagctctcc aagctatgat ccaaacaaaa tgacaaacgg gatctcacaa gaagattaca
     2221 aagcttatga agaaaatcct gaacatccat tcatcagccg atttgcgaca ggttatgctc
     2281 ctggatctac gtttaaaatg attacagcag caatcggtct cgacaacggc actatcgatc
     2341 caaatgaagt gttgacgatc aacgggctta aatggcaaaa agacagttct tggggatctt
     2401 ttaaaacaac gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact gctttgatct
     2461 attccgataa tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa aaatttcgta
     2521 caggcttaga taaattcatt tttggtgaag accttgattt gccaattagt atgaatccag
     2581 cacaaatttc taatgaagat agctttaact cagatatctt gctagctgat actggatatg
     2641 gacagggcga acttctaatt actcctatcc agcaagcagc aatgtattct gtttttacca
     2701 acaatggcac acttgtctat cctaaattga ttgcagataa agagacaaaa gataagaaga
     2761 atgtcatcag cgaaacagca gtacaaacga tcgtgccaga tctgagagaa gttgtgcaag
     2821 atgtaaatgg tacagcacat tctctttctg ctttagggat tccgttggca gcgaaaactg
     2881 gtacagcgga aatcaaagaa aaacaggatg taaaggggca agagaacagt ttcttgtttg
     2941 ctttcaatct tgataaccaa ggttatatga tggtcagcat gttggaaaat aaagaagatg
     3001 atgattcagc aaccaaacga gcatcggaac tattgcaata cctcaaccaa aattatcaat
     3061 aaaaaattgc tttttaagta cagtaaaagg actgtggcgt tatcgtcaca gtcctttttt
     3121 caaataaacg gttttcacga tgtctctttc acaacttctt tttgatttac tcacacttca
     3181 taaacttttt ttgtaaaata tttgagaaca agtggagaaa taagtgtact taataaaatc
     3241 acgataacta aaggtgaata gtactgtggt tcgatcaatt gactttgctg tccaatttgt
     3301 aaaatgatca aagccatttc tccacgcgaa atcataccag caccgaccat caatgaacta
     3361 tgttgagaaa aaccggcaat ctgagcacca aaataacctc caagcaattt ggtcaaaatc
     3421 gctacaagcg tcaatataga aataaagagt agttgttcac taaagtcaga aaaatcaact
     3481 tctaatccta cactgacaaa aaatactggt ataaatactg catatcctaa agcctctaca
     3541 ttgtggtata cttcttcttt aacctttgtc tgactgacag ctatcccagc aaaaaaagca
     3601 ccaatgacag agctaagccc aacaagatcc gctacgtaag acattcctaa acaaataact
     3661 agcgatacga tgataataga agaatttgcg tacacttttt ctgcaaatga aagtaggaaa
     3721 ggcgcaatcc atttgactaa taagaaaata aaaacaaaat aaattagctg ttcaatcaaa
     3781 acaagcggta aagaagcatt gctagaagct tcccccgtta gaaaagatag gctgaaactt
     3841 agaactaaaa cgactaaaat gtcatctacc acagaagcac ccaaaattgt tgatccttct
     3901 tttgtattca ctacgtttag ttcttttaat acttctactg aaatactgac agaagttgcc
     3961 gcaagaataa tcccaaagaa aatcgcttca tttcccgaaa cttggaatgc tttgcctgtc
     4021 aaccagccaa aaacaaccgg gaagatgatc ccaagcaaag caacgaacat accaggtcgg
     4081 aaatattttt tcaaaagaga taaatcactt tccagaccag ctaaaaacat taataagatc
     4141 acaccaatct ccgaaaagtc atggactaga atatcaggtt tgacccaatt caatccagct
     4201 tggcctagca aaacaccaac aagtaattgt ccaatcactg ctgggatacc taatctccgc
     4261 gagatatgtg agccaatcgt tgttgaaatc aatatcaggc ataaaatacc aataaattcc
     4321 attctcatca ttcctttgta taaaatatag cccgaactta gcataacata cattattgaa
     4381 aaaataggaa aatatactca caaaaaaacc aacaatccaa aagatttttt tggattgttg
     4441 gtttttatac ggtctatttt aataactaaa aatgattaac gtatagtcat ttttcttatt
     4501 ctgatgaaat tgataatctc ctggatgccc atgaaaatca aaataaagcc gaatatttgg
     4561 aatagaagca tcaaactttt gaatggattg aacaataaga cgaatccgcc aatcaacaac
     4621 agaatactac tataaacact ccaactttta gcttttgtat tcatgctgaa gaacaactgg
     4681 aacaagctgt tgatgataac agctaatcca agaagaatcg gcaggataga aacgattgca
     4741 ggtgcaaaag catagacgat cagagctaag atcaggaaaa aaagaccact gaacagaccg
     4801 atactccaag aacctgtttt ccttttaaaa cggtaagcct caaaaagatt caacaaccca
     4861 agcaaaacaa gataggcggt gatcagataa ccgataaaat gaaaaacagc agaagggttt
     4921 agcacaatcg caatcccaga aataatataa acagcagctc ttaataaggc gtatttttga
     4981 atattttgtt taatcgtctc aaacatataa gaaaacctcc tttgacttat tttaacgtga
     5041 actagtcatt ttaccaaatt aaatgttaca aaatgagcga aggagaagaa tatctctgga
     5101 aaaatcaact tctgaggatc ttttccattt ttttgccttt tgctagctca tcgatcattt
     5161 tatctaaata acgaatttct ttcatcaagt tatcctcgat gtcttctact cgtacaccac
     5221 aaacaacacc tgtaatcaag gaacgtgaag gattggggac aggggcttcc gcaaaaaatt
     5281 gttcgaaagt cacttgtttt tgtagttgat attctagttc tgtttgggaa taacccgtca
     5341 gccaacaaat gatttcatct acttcctctt ttatacgatc ttttctctcc gcttttgcta
     5401 catacatagg ataaacgctt gcaaaacttg tcgtaaaaat acgattttgc gccataatca
     5461 gtcacctttc tttttttcaa attatttact acttatagta tataagaaaa agagaagaaa
     5521 aagcgattat tcaaaaaaac aaaaaggatt gtgacaaaat tcttgtcacc atcctttttt
     5581 ccgctctctg actttttaca atcttcatag ctcaagtgcg catcagataa tcgaatgctc
     5641 ctaaagctgc agtagctcct gaacccattg aaacaatgat ttgtttgtat ggactatctg
     5701 tacaatcacc agctgcgaaa acgccgggga tattcgttgc gccatttttc tcggtcacta
     5761 tttcatttct tggattcatt tcgatcgttc tatctagcca atccgtatta gggagtaagc
     5821 cgactagaat gaatactcct gaaacagcta gtgtatgcat ctccatcgta cggcgatcga
     5881 tataggagag agattctacc tgattcgtac cgctgatttc tttcgtcgct gcatgtttga
     5941 taacagtgac attcgcaaga gagactagtt tatcctgtaa gatttgatct gcttttaatt
     6001 ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc agccaaatcg attgctgctt
     6061 caacaccaga attaccacca ccgataacag caatctcttt tcctttaaat aaagggccgt
     6121 cacagtgtgg gcaataagca attcctttat ttttaaactc tttttcgcct ggtacattga
     6181 tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt tgctttaagt gatataccgt
     6241 tagctaagcc aatattaata aaatgttgct ctttatttaa ggaaacggcc tgttgattca
     6301 tcataatgtc gatagggtaa ctacgcacat gactttctac ttgctgcatc aattttggac
     6361 cttctgtgta agaggtacca atcatatttt caatccctag tgtttcaacg acttgtccgc
     6421 cgaaagaatc agcgacaagt cctgtacgga ttcctttacg tgctgcatag atagctgcac
     6481 tactagctgc tggtccaccg ccaatcacta atacatcata aggttctttt tcaaacaact
     6541 cttcttcaat caaagggcca gtgatttttt caacaatttg ttcgatcgtc atgcgtccgc
     6601 tactaaattc ttctccattt aaaaacacag ttggcactgc catgattttt ttatcatcga
     6661 tttctttttg gaacatccca ccttcaatca tcgtatgagt aatatttgga tttaatacag
     6721 ctaatatatt caatgcctgt acaacgtcgg ggcaattatg acaagtcaag ctaacataac
     6781 tgtcaaaatg caatgtttgt gttatttttt tgattttaag actcaaagat tcactgattt
     6841 tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa agaggaaaat tcatgaccga
     6901 gggggatacc agcaaaaacg attccacttt ctttgccaac tggattgata gaaaaactag
     6961 gtgtgcgcat cgaatctctt ttttcccaag agattttagg agacatttct gctatttcct
     7021 tcgtaaattc agctagtttt tgactatttt cactattatc caaactaaca gaaaaaacaa
     7081 taggagattc aatcagtgct aaatattgtt cgagattttg tttagtttct ttgtctaaca
     7141 tatccaccac ctaaatcttt ccaacaagat caaaacttgg tttcaatgtt tcgccacttt
     7201 ctttccattt tgccggacaa acttcacctg ggtgggtacg tacatattga gcagcgcgaa
     7261 tcttatcaat cagtgcacta gcgtcacgac cgattccatc tgcattgatt tccatagctt
     7321 gaacgattcc gtctggatca ataataaatg ttcctcgctg agctaatcct tgctcttcat
     7381 ccaaaacatc aaaaatacga gaaatctgat gagacggatc accgatcatc acgtattcga
     7441 ttttaccgat agcatctgat gtatcatgcc atgctttgtg agtaaagtgg gtatcggtag
     7501 agcaagaaaa gacttcaaca cctaaagatt tcaatgtact gtattgttct tgtaagtctt
     7561 ctaattcagt aggacaaaca aaagtaaaat cagctggata gaagcaaaga atactccagt
     7621 tccccataag atctttttca ctgacttcta aaaattctcc ttgatgataa gcatgtgcat
     7681 taaattcttc gatttttttc ccaatcaatg acatagtaaa acctccaata ataattattt
     7741 atttataatt attgtaattt agttggagtt gcaaagtcaa tggatatttt gttatttaat
     7801 aaagattact caaaaacgaa ggttaattat aaaaatagaa gaggtcgcaa gaaaatgcta
     7861 ctgctggaaa tactcgggat aacgttgagt aacagtttcc tgttctttca gcatcatgtg
     7921 taagtgcaat cccgtttgaa atcgagcttc ttctggtttt ttatcaacta aagccttgag
     7981 taaatcataa tgttgttcca tgatcgtttc ccatggaagc ccctgaatgg tcactcgtaa
     8041 ccagcggaaa cgttctaaat gggtattatg gtcatctaac cagttccaaa cttcttttct
     8101 tcctgcaatt tcgaaacaag cttcatgaaa taaattatct gcacggaaaa aagcgcgttc
     8161 gtcatttatt ttgactgctt tttcttgttc ttctaaaatc gtttctagca ctattcgtgt
     8221 ttgtgtgtcc aatttggaac aacattcaat catgatttgc tcttccaact gttctcggac
     8281 aaagcgagca ttttctgcag aaacaagatt aattttggaa acatacgtac cactttgagg
     8341 tatcgtatac acgagttctt gctgacgtag ttgaatcaat gcttcgcgaa tgggagtccg
     8401 gccgatagca agatgttcct ccagtgcctt ttcagatatt ttccttccag gttctaagtc
     8461 agcatagata atttgtttac gaatcgcttg ataagcttgt gattgcaagc tgcttttcat
     8521 ctttattcct ccaaaatcat ttctatgaac gtagtataac agaagaggtc tgaaaaacaa
     8581 tctggtatgt cacattaact agtaaatttg tataaatagt aaaattttgg ttgacatagc
     8641 gatttttatt gcataaaatt cacttgaata caaaaactaa tatgttagtt taggagggga
     8701 aaaatgattg atttaaacaa ctggaggaac tttatccctg aattatctca aaaagaagta
     8761 gaaatacctc attttgaccg tgcatcatta agagaaaatg gacaaaaaaa tcctgtctgg
     8821 atccatttcg gtggcggaaa tctttatcga ggatttcatg cagaaatcgc tcaacgttta
     8881 gcggatctag gcgaattgtc cactggtgtc attgtttgtg aaacattcga tgaagaaata
     8941 attgaaaggg cttaccaaga ttttgatgat ttgattcttg aagtggtcat gccaagcagt
     9001 ggagagttcg tcaaacgtat attagctgcg acagctgaca gtctgtattg tcatccagat
     9061 tgttctgaac actttgataa gatgattcga cactttgaaa atcctagttt acaattagtt
     9121 acttttgcta ttactgaaaa aggttatcga ttgaaagata caaataataa atatatagga
     9181 ccgattgcag tcgatataaa aaatggtcca aagaagccat cacatacaat gagtattctt
     9241 gctgtgctac tttataaacg ttttttatct ggagcttggc cgattgcact tgtctctaca
     9301 gataattttt cacaaaacgg tagtcaatta cgtacttctt tgctaacgat cgcaaaagaa
     9361 tggcaaaagg aaggttatgt acaaaataca tttatccaat acttatcgaa tgaagaaatt
     9421 gttgcgtttc catggacgat gattgatcgt atcaccccga atccttcctt gtcagttagc
     9481 aaaatgttgg caaaagaggg cttttcaaat atgtcgattt tccaaaccgc aaaaggaaat
     9541 aatattgctc catttgtcaa tacagaggac atccattatt taatcatgga agatcatttt
     9601 cccaatggac gtccgaaatt cgaaaaagct ggggtattct tgactgatcg tgcaacggta
     9661 gataaagcag atacgatgaa ggtgacgact tgccttaatc ctttgcatac gtctttagct
     9721 attatgggtt gtttactggg gtataggagc atagctgagg agatgaaaga tccagattta
     9781 tcgctgctag tttctgaaat cgggtacaat gaaggcctcc cagttgtgga gtctccaggt
     9841 atcattgatc caaagatctt tctggaagaa gtaattactg tgcgcttttc taatccaaat
     9901 attcctaata tgcctcagcg aatcgccacg gatacctctc aaaaggtggc gattcgttat
     9961 ggtgagacga ttaaaaaata tatggaaata gaagttaaag acccgcagac acttgtattc
    10021 ataccattga ctctcgctgc ttggcttcga taccttattg gcattgatga tcaaggaaaa
    10081 ccattcatcc cgagtccaga cccactattg acagaccttc agcataagct ttccgatatt
    10141 caactgggta cgcaagatac atcacatatt catgagaaaa tggctcctat tttgtcaaat
    10201 aaagaaattt ttggtgtaga tctttaccaa gcagagcttg gcagaaaaat agaagaaatg
    10261 ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa cattacagga ttacttacaa
    10321 gaacatggag gaaatttatc atggaaatga cttttagatg gtacggatca caggaagaaa
    10381 agattacctt agatgaaatc agacagattc ctgcggtcaa agggattgta ggcacattga
    10441 tggatatacc agtaggagag gtatggccta gggagaggat caaagaacta aaagaagaaa
    10501 ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat gatgccatta
    10561 aaattggatt accttcccgt gagcagtata tcgaaaacta taagcagaca atccgaaact
    10621 tggcagaata tggaatagaa gtcatctgct ataattttat gcctgtattt gactgggtga
    10681 aatctgacct tgactaccgc ttagaagacg gctcatccac gttagctttt atatctgatg
    10741 atattccaga tgatccaaga gaaatcgtcg cgcgcatcga acattccagc aatgaatttg
    10801 gattgccggg atgggaacct gagcgcttag ctcctatcaa atccttgctt gaagcctatg
    10861 catcagtaga cgaagaaaaa cttcgagaaa attttgccta ttttctccaa tcaatcatcc
    10921 caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc agatgatccg ccatactcga
    10981 tttttggatt agctcgaatc gtaaagaatc gagaagattt agattggttg tgtcgcatag
    11041 tagacagccc ttataacgga atcactcttt gtacagggag tattgcagaa gatccagaaa
    11101 atgatgtata tgggatttta gcagaatttt gccaaagaga tcgaatccca tttgcacatg
    11161 ttcgaaatat taaattttta gctaatcgag atttctatga ggcaccacat aaatccgaat
    11221 acggttcatt agacatgtac cgcatactaa aaacaatgta tgacaacgga tttaacggat
    11281 acatccgtcc tgatcatgga agaatgatct ggaatgagac aggaagacca ggatacggct
    11341 tatatgatcg agcattaggt gctgtttatc taaatggatt atgggaagca atcgaaaaag
    11401 gaaacaaaag taaagataat tagtgaaaaa aactgtaatt ttattatgta aataagaagg
    11461 atgttcaaat tcttgataca taaatccgga ggttgttaat aagcattcgg atttatgtat
    11521 cttatttggt ctaggaagta acttgatttt cttttttcga ttgatccatg agtgcttctt
    11581 cttttaattc aaagatatat tctgagatcc tcataatcac ttttttccag tcttctggtg
    11641 tgatgattcc ttttaaaata tagatttttt tgatatgaga aggcaattgt ttcaaagggc
    11701 agtcgctgat aacgatgttg cagttctcca taaaatcatt ttgtattttg atcgattcat
    11761 ttgaaaaaat ttgttctatc ttaacgatca gatctttact gatataaaaa tcacgcgtat
    11821 agttcacacc aatcacaatt cgcggctgtt ttttgtagcg atcatacaaa tgtactaggt
    11881 cttcaaccat ccattgcaca ttttcctttt ctaaaataac tggtaacaag tgttgatttt
    11941 gttctctgaa atccatataa aattgttcaa tggcggtttg gacttctttc aatacgctat
    12001 gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg ttgattgaag ttaagaaatc
    12061 cattataaag tttatttcgt gcgaatgcca atgagtaaag aatcttttct ttttcaggta
    12121 taaacaaatc aaatacagta caaagggact ccaccatttc tcttgaataa agaacatgag
    12181 gaacgtttgc tttttttaaa agtttgtatt gctccaagct tgtttcttcc gattcgattt
    12241 tcgtgataga aagtcgagct aaaatattca ctaagatgcg ttgttcgcga caaattggca
    12301 gattaggtcg caaaatatct acagagagat caataaaaaa gtcaatatat tcatactctg
    12361 tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa attctcttta catattttta
    12421 atagaaaata taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa
    12481 aaaaagtatt catcataaaa gccactactt gcggatcttc atcataaaat tctgtagcag
    12541 tacttgaaaa gatgttccaa taaatatcta atgctgcata ttgaatttgt acctcttttc
    12601 cagaaaaaga ctttctgcca ttattagaaa aggagataga caaaccgtac tatgctaaaa
    12661 atgcattaat ctttttcatt ttcgtataca aataagaggc actatagttt gtttgctgac
    12721 tgaaactgat aatcgaattt gtctgacagc tgaacaaaga agtcaagatc tgataactga
    12781 ctgattcgtt gcagtatctt ccaaatagac gataatagca gtaagccgga tcaattcctt
    12841 caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt agattgagac
    12901 gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa atttgattca gacatatgga
    12961 aatgatctgc taaatattgt gtagtaataa agaaacgatg ttcagatagg aaaagaaaaa
    13021 tatctaattt tgtattgctt tttttcttca agataaacgt tttaatcgct tcttgagttt
    13081 gcatgaaaga ggctcctttt tacttaaatt gttaaaactt gaaaatatta ctgttttttt
    13141 cataaattta gacaaaaaaa cagtaaacat ataggtagaa tctctactat cttatttctt
    13201 gcatacaaaa aaatcaagga gtgagagagt tatgagatat tcaaccatct taatatcaac
    13261 atttgcagcc ttaagcttat cgcttactat aaatcagaca gtccacacag aggagacaga
    13321 aacagccaaa tggacagaaa ccaagtgggc caacgtcaat cgaattacct ttcaggatga
    13381 attttcatcc ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag cgaccatcaa
    13441 agcaaaaatt gactattcgg gagacttagc acctatacta caatcagctg ttacatttga
    13501 gaatgttgat cccactttgt cgattggcta tgacgagaac actttgacta tccaacacaa
    13561 gcaagcgata tttaccttca ccgttacatt aaatcaggac ttgttaaaac cagcgctttt
    13621 tactgtcaag gtatcagatg gactaccgaa cgatcatcat ttaacgccgt atagtcagcg
    13681 gcagacaatt gagcaagatt cagccatcga ctcaggagga gatcttgttg aagaaccaac
    13741 agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc cagacggaga
    13801 acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac cagagccaga
    13861 caataaatca aaacctgaaa taacccctgg agaaaaacca gatgtaactc cagaaccaga
    13921 tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata atgaaactga
    13981 aaatccagaa aaaccagagg tagatccaga agaaaaacca gatgtgactc cagaaccaga
    14041 tacggacgca agagaccaag gaataccaga aaaaataaac aaaaaaacaa ttcaagagga
    14101 tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta aaaataaatg aagagcagct
    14161 gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa
    14221 ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc cggagagtca
    14281 caaaacaaag ttatactgtg gagcggaatc attctattaa gtatagcaac aatgctttct
    14341 gcaaaaagat ttaaacaaaa cagatctctc taacatttga ataatgtctg tgaacgcatt
    14401 tttcaaataa aaagaaatat ccagaaaatg accccaaaat accagattga aatctgatat
    14461 ttggggtcgt tcatacttta tctttcttca tatttatttt aagggatatc tgtttctttt
    14521 ggaagatggt tcgggatctt tttcactttc aatacatcta aaaagaaggt aaagatcgga
    14581 atgccgacaa tcaagcccca aacgccaaag aaccgttcgc taacaagtaa gaccacaaat
    14641 gtataaaaaa tcggcagttc tgttttgctg gacatcaact tcggattcag cacataagac
    14701 tcaattagat ggacaatcaa aatcgtaatc aggatataaa caacatctcg gatgcctcct
    14761 tgggaataag ctatgaaaga tagaggaata caagaaatga taacacctgc tactgggatc
    14821 agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc
    14881 aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa caaattgagc ttctaatacc
    14941 aaaccaaatg tattgacaaa cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa
    15001 aattcacttt ttaaaaacaa tcttgagaat acaattgttt ttttcttttc aatcataaaa
    15061 aagaaactta agatgaatga catagcaaac gccactgcca tttttcctat atcctgtaaa
    15121 tagccaagcg caatcgaagc gccattttga agctgttcaa aaaaatcgtt tcgctcaata
    15181 taagtatgga tatattgtaa aacaggatct gcatcttttg gctgatgctc gtaaaaatca
    15241 ataacggact ggatcatatc aaatgtttga tgaataagca tcgggatata ctttgtgaat
    15301 gccagataga taaggtaaac gatcagtgta taaatcatga tagctaaaac acctgtaggg
    15361 attcggacaa atcgctgaat gatttgaaca agatgaatca tcaaataagt aaagataaat
    15421 gtgagcaaaa tggttgtaat catacttcta actaagaaaa gaacaaaaac gacaaataat
    15481 aaaacagtga gacggcgtat tttttcattc gccaaaaaac gctgataaac ggtcaaatcg
    15541 acaacctcca aataacaaaa aataaacacg ttattcatta tatgatggaa tatgaaaata
    15601 agaaagagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga gttggttttc
    15661 ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg aaaattatct
    15721 agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag tggaagataa
    15781 gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc ttgaggagtc
    15841 agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat ataatttttt
    15901 tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa atcatttcct
    15961 agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag atactgggta
    16021 taggtaagat tcagcggctt gaggagggaa gtgtagtgtc taatgatttc cttagaggcg
    16081 gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt tccacctccg
    16141 ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca ctaactgctt
    16201 tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact ttatgcgctg
    16261 cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct tcaatctcta
    16321 cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca ctgacagtag
    16381 aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta aaacatgcac
    16441 tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag
    16501 ctgcaatatt caattcgaat gtacgatcgg gattatgaac ttctccagag cgtccgcctg
    16561 tgttgatcat tttcgtagaa tacatttttt tcataaaatt accaccttta attatatttt
    16621 atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt ggcataagat
    16681 aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa
    16741 atatcagaga caaaaactaa tctcatcata aacattacat atatttctca aaaagttgtt
    16801 ttctttcttc aaaacttact tcacgatgga cctgatgaag catccataaa taaccaaaag
    16861 gatctctgaa cattgagtta gtcacaccaa agtccatcat ttctgtcatt tcttgaatct
    16921 ctacacagcc atttcgtaat gctttattat gtgtgtcctc gatatcagag acgatgatat
    16981 tgaaccagat cgtatttgga tgttcagcag taggagcagt caaaccaaga gacgtgtttt
    17041 catccagcat atggaatctc gtgccataca aggtaaaaat gacttcatta ttgcctctag
    17101 ggtaatctgt tgcctcaatc agttgtattt catcaaagat tgtttgatac agttctagtg
    17161 ctttcaaact atcatttaat acaaaatcta tttccgttcc tttatgcata aagatctcct
    17221 ctccttttca gtcgaacagt tactagattc ttttattata ccagttaaag aaaacgcatt
    17281 caaaaaaata gcggatttac ttaccaactc attcgattgg atgataagta aatccgcgtc
    17341 ccattacttc tgctacgctg ctgacaatga caaaggcact tggatcgatt tcttctatca
    17401 atggtttcat tcgggaaaat tcatgatcag tcacaacgat caataaaacc tcttttgact
    17461 ttcgattata cccgcctttt gcctctagca gggtaaggcc acgtccaatc tcagcactta
    17521 agcgaaggcg gatgtcttcc aaataatgtt cactcatgac cataacagat ttctttcgat
    17581 tgatacctgt ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat
    17641 aaagaaactc ttctattcca aaaacaaaca gattaagaga aacaacgaat gcgtctgtaa
    17701 aaaataagcc aatcgaagta ttcaagtgga aatatttttt aaaaatgagg ggagggatcg
    17761 tcgtaccgcc gctagaagag ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg
    17821 cacttccaaa aatgacagac aacaaccgat ctgaagtaat cattgtttct ggaactattg
    17881 caatgattaa aggaaaaaac aagcttccga aaagtacttt aaaaaagggt tttttaccta
    17941 aaaatagaag agctagtacc aacataacga tattcaacac taaaataact atagctcggt
    18001 caaatcctaa aacagcttcc agtaatattc ctagaccgct gacacctcca gcagcaatat
    18061 gatgaggccc taaaaacata ttgatagcga ctcctaatat gaagagggca aaaataatct
    18121 taatgtatgt ccatataaat tgtctcgttt ctttcattta tccacttcct taattcatta
    18181 ataatactct tatcttatta taacttactt ttcaccttaa gttaacttta attaataaaa
    18241 aaattaggct agtctaaatt attatgtttc attcttctag aatatacttc tacgttaaaa
//
LOCUS       DARBER010000041        17971 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARBER010000041 DARBER010000000
VERSION     DARBER010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.041"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            2..826
                     /locus_tag="SCK06_001368"
     CDS             2..826
                     /locus_tag="SCK06_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1151382.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            858..2168
                     /locus_tag="SCK06_001369"
     CDS             858..2168
                     /locus_tag="SCK06_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG1151383.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            2316..3575
                     /locus_tag="SCK06_001370"
     CDS             2316..3575
                     /locus_tag="SCK06_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1151384.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            3585..4457
                     /locus_tag="SCK06_001371"
     CDS             3585..4457
                     /locus_tag="SCK06_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1151385.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMILFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            4469..5299
                     /locus_tag="SCK06_001372"
     CDS             4469..5299
                     /locus_tag="SCK06_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1151386.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            5339..7522
                     /locus_tag="SCK06_001373"
     CDS             5339..7522
                     /locus_tag="SCK06_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1151387.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            7583..8803
                     /locus_tag="SCK06_001374"
     CDS             7583..8803
                     /locus_tag="SCK06_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG1151388.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            8800..10260
                     /gene="gtfA"
                     /locus_tag="SCK06_001375"
     CDS             8800..10260
                     /gene="gtfA"
                     /locus_tag="SCK06_001375"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG1151389.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            10337..11443
                     /locus_tag="SCK06_001376"
                     /pseudo
     CDS             10337..11443
                     /locus_tag="SCK06_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            11765..11974
                     /locus_tag="SCK06_001377"
     CDS             11765..11974
                     /locus_tag="SCK06_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1151390.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            11967..12116
                     /locus_tag="SCK06_001378"
     CDS             11967..12116
                     /locus_tag="SCK06_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG1151391.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            12270..12935
                     /locus_tag="SCK06_001379"
     CDS             12270..12935
                     /locus_tag="SCK06_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1151392.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(13219..14304)
                     /gene="xerS"
                     /locus_tag="SCK06_001380"
     CDS             complement(13219..14304)
                     /gene="xerS"
                     /locus_tag="SCK06_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG1151393.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            14638..14871
                     /locus_tag="SCK06_001381"
     CDS             14638..14871
                     /locus_tag="SCK06_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG1151394.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            14868..15275
                     /locus_tag="SCK06_001382"
     CDS             14868..15275
                     /locus_tag="SCK06_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1151395.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(16412..16930)
                     /locus_tag="SCK06_001383"
     CDS             complement(16412..16930)
                     /locus_tag="SCK06_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG1151396.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         6081 a         2791 c         3296 g         5803 t
ORIGIN      
        1 tatgaaaaat aaattttttt caaatgcgcc aatccatatc atgcttattt taggtggtat
       61 tgcaatggca ttacctttta tttggatgat tttaacttca gggaaaacgt tggttgaatc
      121 tacccaaatt ccacctacaa ttttccctga gaaatttcag ctaagtaact atcaacaagt
      181 acttgattta ttaccattca tggcttttta tatcaataca tttttaatgg ttgttggacg
      241 agtgattggc tcggttttat tttcagcaat ggctgcatat gcttgtgcgc gtctaagttt
      301 tccagggaaa aatatccttt tcagcttagt acttattcaa atgatgattc ccggttccat
      361 ttttattact ccacagtatt tattagcaca aaaattgggt attttaaata caattccagc
      421 attaatatta ccaggggtag ttagtgcgtt tggtacgttt ttattaagac aatttttctt
      481 aggtttacct gatgaattgg aggaagcggc ctatttagaa ggtgccaata tttggacgat
      541 ctttttcaaa attatgttgc cgcttgctaa atcaggattg gttgcacttg gaatattcac
      601 agcgttgttt gcatttaaag atttaatgtg gccattgatt gtcaatactt caattgataa
      661 gatgccttta gctccgggat tagcaaactt acaaggacaa tatgccacga acttcccaca
      721 attaatggca ggttcgttat tagcaatttg gccaatagtt gtgatattca tcgttttcca
      781 aagaaaattc attgaaggaa ttgctacaac aggtagtaaa ttataaaact attaattagt
      841 gattggagag taagtacatg acaaaaatta catttattgg tgcaggaagt actatttttg
      901 caaaaaacgt attaggcgat gcgatgttaa ctccaagtct acaagatgca catattgcat
      961 tgtacgatat tgatgaaaaa cgactaaaag aatcaaattt aatgcttaaa aatattaata
     1021 aaaattcgaa tgaaaatcgt gctgatattg tttcttatta tgatcgcaaa gaagcgttaa
     1081 gagaggctga ttttgttatc aatgctattc aagtgggtgg ttataaacct tcaacagtga
     1141 ttgattttga tattcctaaa aaatatgggt tgcgacaaac tattggtgac accatgggga
     1201 ttggtgggat ttttagaacc ctacggacac taccagtaat gttagacttt gctaaagaaa
     1261 tggaagaagt ttgtccaaat gcttggttat tgaactatac aaatccgatg gcgatgttaa
     1321 ccatgggtgt tttaaaagcg acgagtatta agactgttgg tttatgtcat agcgttcaag
     1381 tatgcgtacc agaattgttt gagcatttag gaatagccga tcaatatgac ttaaatgact
     1441 ttcaatggaa aattgcaggc attaatcata tggcattctt gttggaaatt acaaaaaatg
     1501 gggaagattt ctatccaaca attcgtcagt tagctgcaga aaaaccaaat ccacatccag
     1561 attctgtacg ttttgaattg ttgaaacgtt ttggttacta tattacagaa tcaagtgaac
     1621 acaatgcaga ataccatcca tactttatta aatcaaaata cccagaattg attgacgaat
     1681 tagggattcc gttggatgaa tacttacgtc gttgtgaaca acaaattaat gattgggaaa
     1741 gcatgcgaga ggacattgta aataatgggg agttaacaca tacgagaagc cgtgaatatg
     1801 cttcatatat catggattct attacgacag gaactccgtc agtgattgca ggaaatgtct
     1861 taaataaagg cttaatcaca aacttaccga cagatgtttg tgtggaagta ccttgtttag
     1921 tcgataaaaa tggcgtgcaa ccaacctatg tcggtgaatt gccaacacaa ttagctgctt
     1981 taaatcggac gaatatcaat gttcaagaac tgacagtaga agccgcttta acactagata
     2041 aatcaaaaat ttatcaagca gcgtatttag atccacatct atcatctgaa ttgtctttaa
     2101 cagatatcaa aaatttagtg gatgatttaa tcgaagcaca tggggactat ttacctgaat
     2161 ataattaaaa agaagctgaa agagtaagga attttcaatt ccttactctt ttcttagtta
     2221 aatttagtaa aaatattgac aaaacactta gtaaacagtt acattagtgt taacaaaaag
     2281 aaaacgcatt caaattgtat aaggaggagc ataaaatgaa gtggtctaaa aaaattattt
     2341 tagcttgtat agcactagga gcaacgatct ctttgactgc ttgtggaaaa aatcaggaag
     2401 atgcaagcgg taaggtagaa atagagtttt ttaatcaaaa aaaagaaatg actcaaacga
     2461 ttcaggagat tgctaaagat tttgaagcaa aaaaccctga tattcatgta aaagttgtgg
     2521 atgtaccgaa tgctggtgaa gtaattaaaa cacgtatgct tgcaggcgat gtaccagatg
     2581 tcattaatct ttatcctcaa agtatagaat tgaaagaatg ggcgaaagct ggttatttag
     2641 aggacttaac cgaagaaccg tatttagaga atatcaaaaa tggatatgca caacgttttg
     2701 ctattgagga tcgtgtatat agcataccat taaccgctaa tgtctatggt ttttactata
     2761 acaaaactgc ttttgaggaa atggggattc aagcaccaga aacttgggca gaatttgaaa
     2821 aaatagtagc tgagattaaa gatcaaaaca aagtaccgtt tgcgattgca ggagcagaag
     2881 gctggacttt aaatggttat caccaactag ctatagcgac tgctgctggt ggtgaagagg
     2941 aagcgaacaa tgtttggcgt ttctcagaag ttaatggaat taatgctgaa agcaaagaaa
     3001 tgcaaaaaga ttttaatcga ttagatttgt taagagaacc aaaagctttg caaaacaact
     3061 ggcaaggtgc cggttataac gatacagtag ttacttttac aaaaggtgat gcgttgatta
     3121 tgccgaatgg ttcatgggca atgccgatga ttcattcaca aaatccagat tttgaagtag
     3181 gaacttttcc ttttccagca gatgaagcag gtcaaagtct aacgattggc gcaggagacc
     3241 tagccttatc agtttctgca acttctcaac ataaagaaga agccaaacgt tttgtagaat
     3301 atatgacaac acctgaagcg atgcagaaat attatgatgt agatggtgca ccgtgtgctg
     3361 ttgagggcgt tatcgagaat actcaggatt caccattaag tggtttaact gaacttgctt
     3421 ttactgatcg tcatttagtt tggttagcca aagattggaa tagtgaaaat gatttttata
     3481 ccttaacaac aaattattta cacaatggca atcaaaaagc catgattaac gcattaaatg
     3541 ctttctttaa tccaatgaaa gctgacggaa ggtaggaaaa taaaatgaaa ataaaagaaa
     3601 ttttaaatcg ttactgggca atcatttttg tagcaattcc aattattttg cagatgattt
     3661 tgttctactt tccaatggtt caaggtgctt tttacagttt aactaattgg actggtctga
     3721 cttataattt tgattttgtg ggtttgaaca attataagat tcttttttct gatccgaagt
     3781 ttatcaaatc gattggcttc actttaattg tgacgctttg catgattgct ggacaaattg
     3841 gttttggtat tttgattgcg cgggcgttga acgcaaatat taaagggaaa acattcttta
     3901 gagcatggtt cttttttcca gcggtcttat cgggcgtggt tgtttcttta gtatttaaac
     3961 aaatttttaa ctatggttta ccaaaaattg gtagtatgtt aggcattgaa tttttacaaa
     4021 ccagtctttt aggaacaaca tggggagcga tttttgcagt aatttttgtt ttactttggc
     4081 aaggaattgc catgccagtc atcattttct tagcgggttt tcaaagtatt ccacaagaaa
     4141 ttattgaagc tgctagaatg gatggggcga atgataagca aattttttgg aatattgaaa
     4201 tcacctactt attaccaagt atttcaatgg tatttattat ggcattaaaa ggtggcttaa
     4261 cagcatttga tcaaattttt gctttaactg gtggtggacc aagcgatgcc acaacatctt
     4321 tggggttact tgtttataat tatgctttta aaaacaatca gtttggttat gcgaatgcta
     4381 ttgctttagt cttatttatc ttaattggga ttgtttcact ggcacaatta aaattatcga
     4441 aaaaatttga aatctaggag gtttcccaat gaaaacatca aaagaaaaaa atatttctaa
     4501 atacttgctt ttattattag ggggcttcat gattttagtt cccttagttt caacgctatt
     4561 cggttcattt aaaacaacac ccgatattat gaataatttc tttcgttttc ctaatccagc
     4621 gactttggaa aatttccgaa gattgttagc ggatggaatt ggtcattact tttggaattc
     4681 ggcaatcatc acaatcattg cagtagttgc tgtgacagca attattccta tggctgctta
     4741 ttcactagca agaaaaatga ctcgtgggaa agcatacgga ttgatgtatt tatttttaat
     4801 tttaggaatt tttgttcctt tccaagtaat tatgattccg attaccgtta tgatgagtaa
     4861 aattggttta gcaaatatgt ggggattaat cttactctat ctcacttatg ccgtaccaca
     4921 gacactattt ttatatgttg gttttattaa actaaatgtg cctatcagtc tggatgaggc
     4981 tgctgaaatt gatggggcta gtcactttac aacttatttc aaaattgttt tcccaatgtt
     5041 aaaaccaatg catgcgacga ctattattct taatgctcta tggttttgga atgatttcat
     5101 gctaccatta ttaattttaa atcgtgattc taatatgtgg acactaccat tgtttcaata
     5161 taattatacg ggacaatatt ttaacgacta tggtccaagt tttgcttcct atacagtagg
     5221 aattgtcacg attacactag tttatcttgc tttccaaaga agtattatag ctggaatgag
     5281 caatggagca gtgaaataac caataaatta ttctcattat aaaggagttc ataaacttat
     5341 gattatattt gacaatcaca cacaaacatt tcatttacaa aataaaaaaa ttagctatct
     5401 attaggaatc gaagaaaata attatttaac gcatctttat tttggaaaaa agattacgca
     5461 ctattctggt gggtatcgct acccccgaac agatcgttcc ttctcaccca acaatgcaaa
     5521 tgcgcaagat cgtttattat ctttagacac attaccatta gaatttccag gatatggaca
     5581 tggtgatttt agagaaccgg cgcataatat taaattagga aacggtagtc gtgtaaatga
     5641 ttttcgttat gatagctacg aaattcaaaa aggaaagccc agcttagcgg gattgccagc
     5701 cttatatacg gaagatgatg atgaagctga aacgctgatt attcaattga aagacgcgat
     5761 tagtggattg actttacagt tgttctacac aatttatcaa aatcacagtg taattattcg
     5821 ttctacgcaa ttagtaaata atagtgatga aacagtggag attaaccgct tagctagtca
     5881 atcaattgat tttccaaatc gtccactcga gttaattcat ttaaatggta cctgggcaag
     5941 agaacgtcaa atgactagag aaaaaattca tacagggata aaagtattag atagtaaacg
     6001 cggatcaagc agccatcaac aaaatccgtt tgttgcttta gtagatgcca aaacagatga
     6061 atttcaagga gaggcatatg gatttaattt agtttatagc ggtaatcacg aaacggtgat
     6121 tcaaaaagat ccatttgacc aaacacgggt cattatggga attaattcat ttaattttgg
     6181 ttggcaacta aaaactggcg actcatttca aacgccagaa gtcgtgatgg tttatagtaa
     6241 tgaaggtctg aatacgttat cacagacgta tcatgaattg tataatcacc gtttaattcg
     6301 tggaaactat cgtttaaaag aacgtcccac gttaatcaac aactgggaag caacgtattt
     6361 tgattttaac gaagaaaaaa tcatgaatat tgtcgatgat gcgaagaact tgggcattga
     6421 aatgtttgtt ttagatgatg gttggtttgg cggtcgtgaa aatgacttca cttcattagg
     6481 tgattgggtt gaaacagaag gcaaacttgc ttgtggtttg gaaaatttag ccaagcaagt
     6541 gcatgataac gggatgaaat ttgggatttg gtttgaaccg gaaatgattt ctgaagatag
     6601 tgaactattt cgagcacatc cagattgggc attgggtatt ccaggtcgtg accgctcaat
     6661 tagtcgaaat caatatgttc ttgatttttc aagacaagat gttcgtgata atatctatca
     6721 acaaatgacg gcgatttttg atcgtgtacc gattgattat gtgaagtggg atatgaatcg
     6781 aaatatgaca gaagtctatt ctgtcttgtt ggactcaacg atgcaaggtg aagtggctca
     6841 tcgttatatg ttagggttat atgaattttt agaaaagtta acaacgaatt atccaactat
     6901 tttatttgaa agttgttcgg gtggaggcgg tcgttttgat gcaggtttct tgtattacat
     6961 gccacaaact tggacgagcg acaataccga tgccgtggct cgtctgaaaa ttcaatatgg
     7021 aaccagctta gtgtatccaa tttcaagtat gggtgcgcat gtttcagcag tcccaaatca
     7081 tcaaacagga agagaaacga gtttaactat tcgtggcaat gctgcgatgg ctggtgtttt
     7141 tggttatgag ctagatctaa cgcaattaac ttctgaagaa aaagcagaaa ttaaagaaca
     7201 agttgccttt tataaaaagc atcgccaact tttgcaatat ggaacttttt atcgcttgga
     7261 aagtccgttt gaaggcaatg atactgcatg gatgtttgtc tcaaaagacc aaacagaagc
     7321 catggtcttt tatttccgag tattagcaga agctgcgcat ccattagtca cattgaaatt
     7381 agcaggatta gcagaagaag caacttatca aatggaagat gtaacatttg ctggcgatga
     7441 attgatgaat ctcggttttt atattgactc agaactacat ggtgattatg cgacacaacg
     7501 cttttatttg aaacagatat agtagcttga atttacaata taagtgcaac accaaaagag
     7561 actcattcca aacgaagaaa atatgtttta tttacacgca tttactaaaa agcagcctga
     7621 tttgaattgg gaaaatgaag aagtacgtga agaaatctat gcaatgatta actattggtt
     7681 agataaagga ctaggcggat ttagaatcga tgcgatatta aacatcaaaa aaaaactaga
     7741 gtatggcatc tttgaatctg atggagaaga tgggttagcc ttcattgggc cctggattct
     7801 taatcagtcg ggcattgaag tgtggttaaa agaaatgaga gagcggacat ttaaaccaca
     7861 taatagtatg acggtagcag aagccgatgt tcctaatgaa cgtttagatg aatatattgg
     7921 tgaagatggc tattattcga tggtttttga ttttagttat acggatattg atgtacctga
     7981 aactggtgaa tggtttaaag atagccaatg gacatggacg gatatgcgta ccaatatttt
     8041 tacgaatcaa ttagttacgc aagataaagg ttggggcgcc ttatatttag aaaaccacga
     8101 tcaaccacgt tcaattaata aatatattcc agaagaatgg atcaatgatt atagtaaaaa
     8161 aatgttggcg acgcttttta tgttactaag aggtacccct tttatttatc aaggacaaga
     8221 actaggaatg accaacatag aaatggcatc attagaagat tttgatgatg tagctacgca
     8281 ttctcagtat aaacgggcat tagaatatgg attggcacca gagcaagcat tgaaagctgt
     8341 caataaacga agtcgggata attctcgcac acctatgcaa tggaatacgc aaaaaaatgc
     8401 cagattctca acttctgata atgtatggtt aaaagtaaat cctaattatc cagaactcaa
     8461 tgcagaagct cagttaacca atcaacagtc ggtattcaat tattatcatc aattaattaa
     8521 attaagaaaa aatagtgtat acaaagacgt gctaatctac ggacaatttc taccagtgaa
     8581 agataaaaat gaacatattt tgctttatga acgccaatta gaagataaaa tcgtattagt
     8641 tatcttaaac gttacagcaa cagaacaaga atattatgta gatgaacagt accaacaagt
     8701 attaatcaat aattatgaag atgttttatt agatcaaagt cgaaaattgc gtttacgacc
     8761 gtttgaaagt attgtattag ctaattatgg aggacaatta tgaaaattaa aaacgaagca
     8821 atgctcatca cctatgcaga tagtttagga aaaaacatgg gcgaattaaa acaagtctta
     8881 gacaaacatt tacaaggtgt tattggtggt gtacatctgt tgcctttttt tccatcgaca
     8941 ggtgatcgag gctttgcgcc gagtgattat acgacagttg atccagctct tggaacatgg
     9001 gaagatgtgg aagcattagg tgaagattat tatttgatgt ttgattttat gattaatcac
     9061 atttctcgtg aatcaaaatt tttccaagat ttcaaaaaaa accatgagca gtcaccatac
     9121 aaagaaatgt ttatccgtat tcatgaattt ttcccagaaa atcgaccaac acaagaagat
     9181 attgatttaa tctacaagcg taaagacaaa gcgccgtttc aaacggtaga atttgcggat
     9241 ggtacgacag aagaggtttg gaataccttt ggggaagaac aaattgattt agatgtgacc
     9301 aaagaagttg tgaaagaatt tattcgagaa accatcaaag atatggcgaa ccatggctgt
     9361 tctttaattc gattagatgc gtttgcttat gcgattaaaa aattagatac caatgatttc
     9421 ttcgtggaac cagatatttg ggaactttta gacgaagtgc gtcaagaagc agcgaaatat
     9481 gacgtcgaat tattaccaga aatccatgag cattattcca ttcaaatgaa aattgccaat
     9541 catgattatt atatctatga cttcgccttg ccaatggtca ctctgtattc actttatagt
     9601 ggaaaatcag aacgtttagc caattggttg aaaatgagtc cgatgaagca gtttacaaca
     9661 ttggatacgc atgatggaat tggtgttgtg gacgctcgtg atttattaac agatgaagaa
     9721 ttggattaca cctctgagga attgtataaa gtcggggcga atgtgaagaa ggtctattca
     9781 agtgccaatt acaataattt agatatttat caaattaaca gcacctatta tagtgcatta
     9841 ggcgataatg accgtagcta tctgttagca cgtgcgattc aagtatttgc gccagggatt
     9901 ccacaaattt attacgttgg gttattagca ggaaaaaatg atattgaact gttggaagca
     9961 acgaaagaag ggcgtaatat taatcgtcat tactatgatt tagccgaaat tgaagaagaa
    10021 gtgcaacggc cagtgattca acaattattt gcacttttaa aatttagaaa tgaatctgcg
    10081 gcgtttgatt tggatggaac gattgaagta gcaacgccat cagaatcaga aatagtgatt
    10141 acacgtaaaa ataaaaacgg tagtgatagt gcaacattaa cggctaactt ggatacgaaa
    10201 gaatttatca ttgagtctaa agatataaaa attttcacta gtgttgcatt aaaattttaa
    10261 cctattccat agaacaaaaa atcctttata ctagcacttg gatgacttat ccaagaccaa
    10321 taaaaaggac tcatacatgg ataagtataa aacaaataca gcatttaata aatggttttc
    10381 tgccataaaa ttaggaaaac tttctgtcaa ctcccaaaaa tgtatttttg attacaattc
    10441 gtatcgtaaa aaactgtctt ttgaagctgt gctcaagctc tttctctacg cagttaatga
    10501 agaaaaagaa agtttgcgtg atttgagtac agctcttatc gaccgatcgc tccaactaga
    10561 aaccgatgtg tcatcgatta gtcataccca attgtctcga gcatttaatg cacttgaacc
    10621 taaagtgtta gaagaaattt ttcaacaact gttagaaaaa gttcaacatc aaatcaaacc
    10681 aaccaagcga aatcatctgt atctgattga ttccagtacg ttttcgctta gtttaaaacg
    10741 tcataagtgg gcgactttcc gtcaaacaaa atcaggtgtc aagttacact taaactattg
    10801 ttatatggac gatactacga tgtatccaac tgattttacc ttaacaaatg cccatgaaca
    10861 tgatctcaat caactaccag tattggtgaa tcaaccagag gcaacttacg tgtttgatcg
    10921 tgggtattag gactttgaaa agatggatca gatgcattgg gatggctatt tcttcgtcac
    10981 tcggattaaa aaaaacacca aagttcacgt gatagatact ctggaaacat cgccagaaac
    11041 agaaattcta cgagacgagc tggttcgttt aggatctaaa acctatctaa ccgccaattt
    11101 ccgactagtc actgttcaag ataagaacgg acgtgtgttt caatttatca ccaatcgaat
    11161 ggacgtctct tcaaaagaaa tctctgatat gtatcacgct cgttggcaaa tcgaattgtt
    11221 tttcaagcac atcaaacaac acatgaccat taaaaccttc ttttctcaaa gtgaaaaagg
    11281 cgtgcagaat caattgattt taacgatgat ttccgctctc ctcacgtttc tcatcaaatt
    11341 ggagacgaaa acggaaaaat cagtgttcca aatcaaacga ttctttcgtt atttactgtt
    11401 tcagcccttt gaatgttgtc tagaaaaatt aattcccaca tgagaaaaaa gaaatttggc
    11461 tcgttacttt tggttttcag ttgaataaca gataaaaaat tttctggaaa aagtcatcct
    11521 ctgcgtcagc acagtttttt ttgacttatt ttcagaggaa atagttttca gaatattcta
    11581 aaatgtttta tgcaacacta gtgatatttt ttagaattag atttctaatg tgcatttttt
    11641 gagtattaag gagcaagtat acaaagattt ataaagctga ttaaatttga ataaaaatat
    11701 gcaggaacgc tactattttt agtagtgttc ttttttatat ttgaataaaa aatgtggtgg
    11761 ttaaatggtg gttataacaa aaaatgaatt aatgaaatta ggttatggga agtatcagtc
    11821 tgagagaatc atacgacagg caaaagcaat tatggtttca aaaagctata cctattacga
    11881 aaatagaaga ttaggactag tacctgttga atctgtagaa gaagttttag gtatgaagat
    11941 agaggtagag gaggattttc agaatcatgg ttaattttaa aaaagataaa aatgggaaat
    12001 atcattttac tgctagtttg ggattcgaca aacgaacagg gaaaagagta caaaaaaacg
    12061 tagaggggtt aaaacagtta aagaagaaaa aaagcgtata ccgaacttgt ttctaattat
    12121 ggaaaagaag cagaaacttc tcaaagtact atgttgtttg aggagttttt ttataaatta
    12181 ttcctacctt attatcaagg gaaagtagaa gaaagaacat ataaaaatcg gttatctaca
    12241 atcaaaaaaa cttttcttat ttctttaaaa tggagttaag agaaatacgt ccaattgaca
    12301 ttcaaaagtg gcaaaataat ttattgagca aatataaaaa ttcttatgta agaaacatgt
    12361 atgggatttt tcagatgtca ttagatcgag cagttgtttt gggactactt gaaaaaaatc
    12421 ctgcaaaagt tattggaaat gtgaagaaaa ggaaaactac agttgatttt tggacaaaag
    12481 aagaatttac aaaagttatt tcaacttttt atattgatga tttgtaccaa cattttggtt
    12541 ttatttgtat gtggttactt tttatgaccg ttatgcgttt gggagaagcc actgcattaa
    12601 cttggaaaga tattgatttt gaaaataaaa acttgcgtgt gaataaaaca ctctattata
    12661 agagagccaa aacctatgag tttgtagaac ccgaaacggc ttctagtatt cgtacaatcg
    12721 aactagacga caatacaatt acctttttaa aagcttggaa ggagcgtcaa gtttcaatag
    12781 gaggcatgtc gtttgtactt tcttataatg gaacacctac tcagaaatat acaatccgtc
    12841 atattatgag aagacatgca acgatcgttg gtgttcatat aatccgcata cacggattaa
    12901 ggcatagtca tgcttctttg cttatatcac aataaaaaga tgcacttcga attaaagaaa
    12961 ggttaaaaca tgctgatgta caaacgactt taggaactta tggtcatttg tatctaaatt
    13021 cattgagaga gataacaaat gatcccacag attatatcca aactaatttt tctaaaatca
    13081 aaccgacacc tatttacgat aataaaaaat aaatgataaa caaaagtagg atcacaccaa
    13141 aagaaacaca ctagaaaaac tataatacag gcttttctag cgtgttgttt cttatttccg
    13201 cctatttata cattatactc ataaagcatt taatgcatcg atcgtagccg cattgtctat
    13261 atgggtatat aaagttgttg tattggtact tgaatgtccc aattgttggg cagttaatac
    13321 caatgaattt gtctgttggt agagtctagt agctaatgta tgacgcagtt tgtgagggct
    13381 gacttgaatt ctaaagcctt ttgaatattt tgctaccatc cgttcgattg caataccgga
    13441 tatacgcttg gcttctcctt ggtaaattgc tagaaataaa gctttttcgt tttctgttac
    13501 tttatatcgt tctttccgaa tagctagata attagctaaa tattccattg cgaatgctgc
    13561 tatattgaca gcatctcttt tatttccttt tctagttact accacattat tcttatctaa
    13621 agataggtca tctaaattaa tatttataac ctcggagagt ctcaatccag atcctaaaat
    13681 cagtgcattg atcgccaggt ctctttctac atctcgttga taatagatca aagctcgttt
    13741 actcaaagta tttttatatt cattggcaat ataatctaga taattttgtg tatccttatc
    13801 tagaaataat tttttctcaa tggcattggc acgtgcagct aacgtagttt tatctgtgat
    13861 ggttgctact tgatccatag gattattgtc taagtatggt tttcctagag gattattgga
    13921 acttttcgaa agatatttaa atagaacctt taatgccgtg atatttctat tgatagtaga
    13981 agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct ctttttttac
    14041 attttctttt gtgcgacgtt tcaaatgaga tttgtacaag tttaggtcat aggcagttaa
    14101 ctgtcttatc gaggtgatag aagtatcagg aacgatagac tcactcagaa gccattcata
    14161 aaagagctta taatttttta gatattctaa agtggttcga ggtgattgat ctttatcctc
    14221 acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg tatctatttt
    14281 ttgatatatt tctgtatttt tcatgtcatt catcctttca ataagtaggg gagaagagta
    14341 tctctataat tggactatac caattataga atgttttgtc aattggtatg ctctaaaatt
    14401 tattaatttg tttcaaaaaa agcatataaa caggtatacg tttaataatt taaaataaat
    14461 ggtatacata aatagggaag gaaatagaag ggagcaatct aaattttttg aattttctta
    14521 attaattata tagaaagaac aatctttcta tagagttaga catgagaggg actatgcttg
    14581 atttaaaatc aacaagcgta tatactgtat atacaaagaa aaaggaggga gaagactatg
    14641 gcaattaaag aaagaaaatt tagggaagtg gggaactcag tagtattaac tatttcaaag
    14701 gagtttctta aagaaaatgg gttaaatccc ggagatacgg tttttttaga tgaagaaaaa
    14761 cttcgagcgg caattacaaa aaaacaagcc ttatctgatg aaattgattt agcaatatct
    14821 caagcattaa ctgaatatga tactactttt agagagcttg tcgataaatg agtattcatt
    14881 acctagaagc caaagatatt ataaaaatga atgtaatcca aataaaaaaa tactctccta
    14941 atgaacccct tggtgtaaag gatgcgaatg ctttagaaat gtgtgtaggg caacttcgag
    15001 cctcagcttt tggtgaagag gtttatcctt cgatttatga aaaagcagct atcctactta
    15061 tacaattgat aaagaaacat ccttttcaca atgcaaataa acgtactgct ttcttagcta
    15121 cttttgtctt tttgaaaatc aatggacagc ttttagctat tgatcaaaaa gatgctgtaa
    15181 atttagcggt atatattgct acgtatgata aggatttcga tcaactaaaa tatgaagtga
    15241 tcaacactat aaagacacat acgcagccca tttaaggagt tactgtatgt gtctttatag
    15301 ggttattagc tgctatagcg aaataattga agtaaattac aaatagctat aattcaaatc
    15361 caactatata gaaaacgtaa attttctata tagttggatt aagataacga ctatttattt
    15421 tatcatattt ttactatacc ctatactttt acttggtata tttatgaata atcgcaaatt
    15481 tacccctata ctactagttt gtaagttaga ttaataaaaa tactaaagtt atttgttctt
    15541 ttattaaata cacctacaac ttataaattg atggtattta gataacacga aagaataaaa
    15601 ataacagctg atagtaaagt ttgttgacga ttgctactca atcgaaagtg agttagtgcg
    15661 tatgaacttg cttctgaaaa cttatgaaat gaaagacctt ttacatcttg cagaaatcgg
    15721 tactgggatt aaactaagca ataaaaacta atgttcccct cattctttga acttctacta
    15781 tttgatttat ttattctgat aaatgacgat ttcaccttca ggatcttcaa atcccgtata
    15841 gaatttattt aagatctctt cagtatcagt aataggatag gcgataggcc acataccctt
    15901 tgatggttag aataaactgc tcaaaatctg tatcgtatgt tggctttagc atattaacaa
    15961 aggctatctc caaattataa accataagcg ttgaatcgtt cttaacaatt tcaattcttc
    16021 ctaacccaaa gtcagaagat ataattaccg gacttgcctt acccttcatg tgggttgttg
    16081 agattccata tttaaaggga attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa
    16141 taaaaaaatc tttcccctgc tagtctccta agtccctttg tagcggatat ggcaccataa
    16201 tctcatttta tcgaagtgag tcatggttta tctgttggcc aaccaaaatt gatacaatct
    16261 aacgatgcac cctctacggc attctagggg agtgcacttg cattcaccca ataaaaaccg
    16321 gcagttagca aagaacttat gtagtcgtat gttcttactg ttaacataca agaggtctcc
    16381 tttccacaat tgcttagcag taaacgggaa tttacaaagc taatctcttc cattttctta
    16441 tctttgttct aaaagtccca aatggagcaa ctgtatttac atgaataaat ttatatactt
    16501 cccatacagc cgttttggtt gcctcgtctg cccattttct tttgtgtgca gtgaatagat
    16561 cttcatcact cattgaatca atcatcatgt taatatcatc aatattacga ttgagaattt
    16621 cctctaattc tttcaatggt agatgtgcat aggtatttgt aaaccactta tataactctc
    16681 ctaactgatt ccatttaaat tgatcagaag gcgttttaac agataatcca tttctttcat
    16741 cattttccca ttttagaatt aaattcgtcc aaccaacttg atatgctaaa ttttcagcag
    16801 gtgttctatc cacttcctca actcttttat ccttcaattc ttcaggaata gtttcaaatt
    16861 ctaaaatata tttagtataa gtcctattta tttctttttt taattcttct ttatttttat
    16921 atgttctcat tatattacct ccataaattc ctaattgtct cctttaattc atctataatt
    16981 tcatccacga tattttaatc cgttctacaa tgcatatctg atatgcattg actgtctaac
    17041 tcatatgtga atgatttata aaatatccca acgtctcatc taaagaaatg ttgatcgtca
    17101 gcaaggagct aaaaaatacg aatatatttc ttttgtagta aaatcggact ggtttgcata
    17161 tcattttctt cgtaaatatc aaaatcatgt taactactat caattactaa taaaaactac
    17221 cactttaagc gttaagaact gttaagttta gttaagtgag gatagttcac aaaatccatt
    17281 gttaaacttc acgaaagtgg taaaactcaa aatgctttat ataggattac agcatcgctc
    17341 tttcttcgat tgcgcgttgg gtaaaaaata ttatgaagta aaaattgacg atgacacgtt
    17401 ccttacggct catcagatga aacagctaca aaaacgaaat gatcaactcg aagaaaattt
    17461 aattctaaaa aagcgattgc catattcacg cctcgctcga cgaactatta aaggtcaaga
    17521 tttacctgaa gatcatcctt tttaggaaat tgaagctttt tatgttgcac aatgcattta
    17581 taatactaca atgatgtttt caccagatat tcttctaggg tgcggtgtaa tgaaacagat
    17641 acacttgcaa aagaaaattc accgcgaatt tactaaaatt atgaataatt atgttaatct
    17701 tccgaaattt ttttccaaaa ttaagggatc aagctggtgt tatagattgt ttaaggttgg
    17761 caaaaaaata acttcttttt aaacaattat agaaaaccga tactctgtta agttggttga
    17821 cacggtgtcg atttttatga ctttcttatt ctgaaacttc actaacggac agtacctaaa
    17881 aagtctatag tatggacgtt tacgagtact attttatcaa aaatgctgct catttcaact
    17941 tatatgtttt agtgaactac ccccacttag c
//
LOCUS       DARBER010000042        17594 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARBER010000042 DARBER010000000
VERSION     DARBER010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.042"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..392
                     /locus_tag="SCK06_001384"
     CDS             162..392
                     /locus_tag="SCK06_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG1151397.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="SCK06_001385"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="SCK06_001385"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1151398.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="SCK06_001386"
     CDS             1803..1979
                     /locus_tag="SCK06_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1151399.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="SCK06_001387"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="SCK06_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG1151400.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="SCK06_001388"
     CDS             2474..2941
                     /locus_tag="SCK06_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151401.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="SCK06_001389"
     CDS             3026..3910
                     /locus_tag="SCK06_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1151402.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="SCK06_001390"
     CDS             4024..4260
                     /locus_tag="SCK06_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG1151403.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="SCK06_001391"
     tRNA            4348..4428
                     /locus_tag="SCK06_001391"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="SCK06_001392"
     tRNA            4435..4506
                     /locus_tag="SCK06_001392"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="SCK06_001393"
     CDS             4631..4801
                     /locus_tag="SCK06_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151404.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="SCK06_001394"
     CDS             5021..5728
                     /locus_tag="SCK06_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1151405.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="SCK06_001395"
     CDS             complement(5788..6432)
                     /locus_tag="SCK06_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG1151406.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="SCK06_001396"
     CDS             6559..6846
                     /locus_tag="SCK06_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151407.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="SCK06_001397"
     CDS             6954..7274
                     /locus_tag="SCK06_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1151408.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="SCK06_001398"
     CDS             7355..8668
                     /locus_tag="SCK06_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1151409.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="SCK06_001399"
     CDS             8665..9384
                     /locus_tag="SCK06_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG1151410.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="SCK06_001400"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="SCK06_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG1151411.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10285..12819
                     /gene="secA"
                     /locus_tag="SCK06_001401"
     CDS             10285..12819
                     /gene="secA"
                     /locus_tag="SCK06_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG1151412.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12945..14046
                     /gene="prfB"
                     /locus_tag="SCK06_001402"
     CDS             join(12945..13016,13018..14046)
                     /gene="prfB"
                     /locus_tag="SCK06_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG1151413.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14151..14837
                     /gene="ftsE"
                     /locus_tag="SCK06_001403"
     CDS             14151..14837
                     /gene="ftsE"
                     /locus_tag="SCK06_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG1151414.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14821..15714
                     /locus_tag="SCK06_001404"
     CDS             14821..15714
                     /locus_tag="SCK06_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151415.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15866..17314
                     /gene="cls"
                     /locus_tag="SCK06_001405"
     CDS             15866..17314
                     /gene="cls"
                     /locus_tag="SCK06_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1151416.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5878 a         2967 c         3810 g         4939 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10141 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10201 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10261 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10321 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10381 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10441 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10501 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10561 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10621 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10681 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10741 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10801 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10861 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    10921 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    10981 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11041 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11101 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11161 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11221 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11281 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11341 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11401 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11461 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11521 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11581 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11641 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11701 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11761 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11821 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    11881 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    11941 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12001 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12061 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12121 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12181 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12241 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12301 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12361 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12421 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12481 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12541 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12601 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12661 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12721 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12781 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12841 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    12901 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    12961 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13021 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13081 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13141 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13201 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13261 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13321 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13381 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13441 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13501 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13561 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13621 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13681 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13741 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13801 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13861 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    13921 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    13981 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14041 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14101 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14161 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14221 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14281 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14341 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14401 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14461 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14521 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14581 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14641 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14701 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14761 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14821 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    14881 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    14941 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15001 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15061 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15121 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15181 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15241 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15301 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15361 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15421 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15481 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15541 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15601 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15661 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15721 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15781 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15841 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    15901 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    15961 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16021 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16081 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16141 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16201 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16261 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16321 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16381 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16441 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16501 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16561 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16621 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16681 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16741 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16801 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16861 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    16921 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    16981 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17041 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17101 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17161 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17221 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17281 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17341 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17401 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17461 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17521 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17581 ttttgattcg tttt
//
LOCUS       DARBER010000043        16953 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARBER010000043 DARBER010000000
VERSION     DARBER010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.043"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(388..1854)
                     /locus_tag="SCK06_001406"
     CDS             complement(388..1854)
                     /locus_tag="SCK06_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1151417.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2075..3154)
                     /locus_tag="SCK06_001407"
     CDS             complement(2075..3154)
                     /locus_tag="SCK06_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1151418.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            3262..4665
                     /locus_tag="SCK06_001408"
     CDS             3262..4665
                     /locus_tag="SCK06_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1151419.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            5014..5646
                     /locus_tag="SCK06_001409"
     CDS             5014..5646
                     /locus_tag="SCK06_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG1151420.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            6234..7673
                     /locus_tag="SCK06_001410"
     CDS             6234..7673
                     /locus_tag="SCK06_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1151421.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7968..8186
                     /locus_tag="SCK06_001411"
                     /pseudo
     CDS             7968..8186
                     /locus_tag="SCK06_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8435..8650
                     /locus_tag="SCK06_001412"
     CDS             8435..8650
                     /locus_tag="SCK06_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151422.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8867..9123)
                     /locus_tag="SCK06_001413"
                     /pseudo
     CDS             complement(8867..9123)
                     /locus_tag="SCK06_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9310..10221)
                     /locus_tag="SCK06_001414"
     CDS             complement(9310..10221)
                     /locus_tag="SCK06_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1151423.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(10240..11244)
                     /locus_tag="SCK06_001415"
     CDS             complement(10240..11244)
                     /locus_tag="SCK06_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1151424.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(11241..13364)
                     /locus_tag="SCK06_001416"
     CDS             complement(11241..13364)
                     /locus_tag="SCK06_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1151425.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13369..15816)
                     /locus_tag="SCK06_001417"
     CDS             complement(13369..15816)
                     /locus_tag="SCK06_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1151426.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15803..16192)
                     /locus_tag="SCK06_001418"
     CDS             complement(15803..16192)
                     /locus_tag="SCK06_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1151427.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(16252..16755)
                     /locus_tag="SCK06_001419"
     CDS             complement(16252..16755)
                     /locus_tag="SCK06_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1151428.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16768..>16953)
                     /locus_tag="SCK06_001420"
     CDS             complement(16768..>16953)
                     /locus_tag="SCK06_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151429.1"
                     /translation="TNAQSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNA
                     SGVKDVLLSLFNRIVGA"
BASE COUNT         5110 a         3415 c         3152 g         5276 t
ORIGIN      
        1 gaataaatgg tgttcaatca gctgaccttc agcattagct caaaaagcgg caaaatatga
       61 ggagctattt ttccgtttgt ttctctaata ctcaggtcaa atcgctgatt ttacaacctc
      121 ttccttaaac ggtgttccaa agcttgcatc tgcggtaata agcccagcca aacgaaaaat
      181 atgaggggct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
      241 cctctgattc acggtgttcc agaagtagct tcttcgaaaa acaagccgaa acttcacaaa
      301 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
      361 caacctcttg taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat
      421 ttttttgttt ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac
      481 gattggtgtg atgatattcg taaataattg gaaccaccat gctttaggat cagcagtata
      541 atctactttt ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc
      601 accaaagata aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt
      661 ataagcttta ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac
      721 aactgagttc aagttcgtca accattttac taattcattc atatctttga taccaaaaat
      781 cggcaacaaa ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt
      841 acctttacga ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa
      901 caagattttt agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt
      961 tgtcaatgcg taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg
     1021 aaaagcactg tatgccccat ttgccatcaa gtctttagga atattatcac tagcaaacag
     1081 catacccatt gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat
     1141 catcccttta ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt
     1201 ttccgcccct ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa
     1261 tgttggaata tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt
     1321 gatgaacggt gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt
     1381 tccggccagt ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa
     1441 ggcaagaaat ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat
     1501 atttccattt ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc
     1561 caaataagga atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt
     1621 agatgtcgta ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc
     1681 taattgtcca acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga
     1741 ggtgacaact gatactcctt gctgagcata gttattaact acattcccca tgccccaaac
     1801 catgttaaat gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc
     1861 tctcctttat gataaaaatt cgtaaatttc aagtttaagt tagccactac aggtaactca
     1921 tttgtttgat accgtaactg cctcgttatt ctaaatcagt gaggccttga taatgaactg
     1981 aggtatgata gtcttcagac gattctggcc ccaggccagc tttccagaca tgaagccatg
     2041 tcccgagtga ggatcgttat caaggaataa tgggtcaagc caccaaccgt tcttgcttga
     2101 atgcctggac gaatgtatca tgtggtgtcg cataacccaa aattttccgc gggtaatcgt
     2161 tcatccaacg ctggatccgc aaaatctggt tctcgctcac ctcactaatc gggcttccct
     2221 tggggacgaa acggcgaatt aatttgtgct ggttttcgct cgtcccacgc tcccaagaag
     2281 cataggggtg gctgaaatag acgtcagcta catcttgcag agcatcgtgt agcccagaaa
     2341 actccgagcc attgtcggat gtaacggtct taaataattg atcgaagtta gcgccagccc
     2401 gttctcttag ctgttgaacc gcttggtcca ccgattcggt gtccttcccg ttcaacttta
     2461 ggatgacttc aaagcgtgtc tgccgttcag ccagcgtcag cagcacggca tccgacttgg
     2521 tcttgtttcc cactaccgtg tcgatttccc aatgaccgaa cgtttcccgt gtttcaactt
     2581 ctttagggcg ttcttcaatt gacgtcccga gaacacgctt gttgggacgg tgtcctgggc
     2641 ggaccgcttt gattttacgg gacagttttt caagcaagtc catgttcttt gttcgcatga
     2701 ttccgcggtc aatccattga taaagagcgg ttgtgccagg aatgatggca caatcgaaca
     2761 gctcgtgttt tctggcaaat ccgaccacga cgtccggcga ccatttttca tttaacattt
     2821 tgtcatctgc ccaatcaatg aaggcatttg tgttcgccca tttggggcgt ctgccacagt
     2881 tcagtcgttg cttttcatag aaggcctgtc ctgcatcagc gtcataaaca gaatagtagg
     2941 tgtcatacac ttttccattt tgcgtttggc gttttaactg ggtgatggtt ccgcgatcaa
     3001 tctcattgtt tatggtttgc ggaacacgtc ccagcacttt ggcaacctga cggttggaat
     3061 agccttcctt tttcaggata gcgatttggc aacgttctga gtaggaaaga tgttttccct
     3121 tgcgtgaaac tgtgttatca tgggtttgcg tcatgtgaat tcatccttgt ctattgagag
     3181 ttgtggtaac ttcaatataa catggaattc acgtggcgtt ttttttattt taaatggcta
     3241 agttcattat aaaatccgcc aatgaaaaaa tctttaaaac ttgcatcagc agttatatta
     3301 atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa ttcaacatac
     3361 aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt aacagcagag
     3421 agtagtgaac ttttgacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
     3481 tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttcg
     3541 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
     3601 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
     3661 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
     3721 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
     3781 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
     3841 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
     3901 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
     3961 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
     4021 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
     4081 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
     4141 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
     4201 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
     4261 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
     4321 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
     4381 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
     4441 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
     4501 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     4561 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     4621 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     4681 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     4741 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     4801 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     4861 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     4921 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     4981 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     5041 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     5101 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     5161 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     5221 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     5281 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     5341 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     5401 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     5461 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     5521 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     5581 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     5641 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     5701 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     5761 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     5821 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     5881 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     5941 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     6001 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     6061 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     6121 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     6181 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     6241 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     6301 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     6361 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     6421 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     6481 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     6541 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     6601 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     6661 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     6721 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     6781 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     6841 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     6901 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     6961 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     7021 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     7081 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     7141 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     7201 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     7261 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     7321 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     7381 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     7441 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     7501 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     7561 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     7621 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     7681 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     7741 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     7801 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     7861 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     7921 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     7981 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     8041 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     8101 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     8161 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     8221 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     8281 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     8341 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     8401 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     8461 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     8521 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     8581 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     8641 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     8701 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     8761 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     8821 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     8881 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     8941 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     9001 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     9061 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     9121 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     9181 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     9241 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     9301 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     9361 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     9421 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     9481 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     9541 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     9601 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     9661 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     9721 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     9781 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     9841 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     9901 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     9961 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
    10021 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
    10081 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
    10141 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
    10201 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
    10261 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
    10321 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
    10381 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
    10441 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
    10501 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
    10561 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
    10621 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
    10681 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
    10741 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
    10801 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
    10861 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
    10921 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
    10981 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
    11041 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
    11101 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
    11161 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
    11221 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
    11281 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
    11341 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
    11401 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
    11461 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
    11521 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
    11581 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
    11641 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
    11701 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
    11761 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
    11821 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
    11881 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
    11941 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
    12001 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
    12061 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
    12121 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
    12181 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
    12241 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
    12301 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
    12361 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
    12421 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
    12481 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
    12541 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
    12601 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
    12661 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
    12721 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
    12781 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
    12841 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
    12901 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
    12961 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
    13021 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
    13081 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
    13141 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
    13201 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
    13261 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
    13321 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
    13381 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
    13441 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
    13501 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    13561 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    13621 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    13681 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    13741 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    13801 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    13861 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    13921 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    13981 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    14041 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    14101 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    14161 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    14221 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    14281 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    14341 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    14401 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    14461 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    14521 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    14581 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    14641 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    14701 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    14761 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    14821 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    14881 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    14941 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    15001 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    15061 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    15121 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    15181 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    15241 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    15301 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    15361 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    15421 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    15481 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    15541 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    15601 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    15661 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    15721 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    15781 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    15841 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    15901 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    15961 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    16021 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    16081 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    16141 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    16201 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    16261 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    16321 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    16381 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    16441 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    16501 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    16561 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    16621 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    16681 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    16741 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    16801 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    16861 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    16921 aattgccagt aagactaagg attgggcatt ggt
//
LOCUS       DARBER010000044        16568 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARBER010000044 DARBER010000000
VERSION     DARBER010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.044"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(29..727)
                     /locus_tag="SCK06_001421"
     CDS             complement(29..727)
                     /locus_tag="SCK06_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG1151430.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="SCK06_001422"
     CDS             complement(720..2072)
                     /locus_tag="SCK06_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG1151431.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="SCK06_001423"
     CDS             complement(2295..3695)
                     /locus_tag="SCK06_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG1151432.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="SCK06_001424"
     CDS             3856..4593
                     /locus_tag="SCK06_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG1151433.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="SCK06_001425"
     CDS             4718..5197
                     /locus_tag="SCK06_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG1151434.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="SCK06_001426"
     CDS             5194..5961
                     /locus_tag="SCK06_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151435.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="SCK06_001427"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="SCK06_001427"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1151436.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="SCK06_001428"
     CDS             complement(6864..7466)
                     /locus_tag="SCK06_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1151437.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="SCK06_001429"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="SCK06_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="SCK06_001430"
     CDS             8147..8731
                     /locus_tag="SCK06_001430"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1151438.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="SCK06_001431"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="SCK06_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG1151439.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="SCK06_001432"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="SCK06_001432"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG1151440.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="SCK06_001433"
     CDS             10632..10811
                     /locus_tag="SCK06_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151441.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="SCK06_001434"
     CDS             10833..11858
                     /locus_tag="SCK06_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG1151442.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="SCK06_001435"
     CDS             12033..13277
                     /locus_tag="SCK06_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG1151443.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="SCK06_001436"
     CDS             13430..14377
                     /locus_tag="SCK06_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1151444.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="SCK06_001437"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="SCK06_001437"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG1151445.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="SCK06_001438"
     CDS             complement(15279..15521)
                     /locus_tag="SCK06_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151446.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="SCK06_001439"
     tRNA            15642..15714
                     /locus_tag="SCK06_001439"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="SCK06_001440"
     CDS             complement(15833..16471)
                     /locus_tag="SCK06_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1151447.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5648 a         2951 c         3331 g         4638 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaa
//
LOCUS       DARBER010000045        16189 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARBER010000045 DARBER010000000
VERSION     DARBER010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.045"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(29..526)
                     /locus_tag="SCK06_001441"
     CDS             complement(29..526)
                     /locus_tag="SCK06_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG1151448.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(656..1366)
                     /gene="budA"
                     /locus_tag="SCK06_001442"
     CDS             complement(656..1366)
                     /gene="budA"
                     /locus_tag="SCK06_001442"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG1151449.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1379..3043)
                     /gene="alsS"
                     /locus_tag="SCK06_001443"
     CDS             complement(1379..3043)
                     /gene="alsS"
                     /locus_tag="SCK06_001443"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG1151450.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3251..3913)
                     /locus_tag="SCK06_001444"
     CDS             complement(3251..3913)
                     /locus_tag="SCK06_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1151451.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3923..4738)
                     /locus_tag="SCK06_001445"
     CDS             complement(3923..4738)
                     /locus_tag="SCK06_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1151452.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4999..5442)
                     /locus_tag="SCK06_001446"
     CDS             complement(4999..5442)
                     /locus_tag="SCK06_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG1151453.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5576..5914)
                     /locus_tag="SCK06_001447"
                     /pseudo
     CDS             complement(5576..5914)
                     /locus_tag="SCK06_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5902..6279)
                     /locus_tag="SCK06_001448"
     CDS             complement(5902..6279)
                     /locus_tag="SCK06_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1151454.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6503..7696
                     /locus_tag="SCK06_001449"
     CDS             6503..7696
                     /locus_tag="SCK06_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1151455.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7862..8284)
                     /locus_tag="SCK06_001450"
     CDS             complement(7862..8284)
                     /locus_tag="SCK06_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1151456.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8867..9361
                     /locus_tag="SCK06_001451"
     CDS             8867..9361
                     /locus_tag="SCK06_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151457.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9523..10695)
                     /locus_tag="SCK06_001452"
     CDS             complement(9523..10695)
                     /locus_tag="SCK06_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1151458.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10901..11716)
                     /locus_tag="SCK06_001453"
     CDS             complement(10901..11716)
                     /locus_tag="SCK06_001453"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1151459.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11866..12312)
                     /locus_tag="SCK06_001454"
     CDS             complement(11866..12312)
                     /locus_tag="SCK06_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1151460.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12309..13313)
                     /locus_tag="SCK06_001455"
     CDS             complement(12309..13313)
                     /locus_tag="SCK06_001455"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG1151461.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13353..13682)
                     /locus_tag="SCK06_001456"
     CDS             complement(13353..13682)
                     /locus_tag="SCK06_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1151462.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13808..16135)
                     /locus_tag="SCK06_001457"
     CDS             complement(13808..16135)
                     /locus_tag="SCK06_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1151463.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4624 a         3236 c         2876 g         5453 t
ORIGIN      
        1 ctcttctttt ttgtttcaat agacgatttt agttattaat aaatgaaaaa gcatttatat
       61 taaaatttgc taataaaaga tctacatatt tttctagaaa tgcttgatcg atttcatcat
      121 accctttcgt tactccgcaa tcgatatcta aaacaccgat taaattacct tcttttacca
      181 ttggcactac gatttctgat tgtgcggctg aatcgcagga aatgtaattt gcatgggttt
      241 tgacatcatc aacgatcaat gtttcttgtt tttcagctga ttctccacaa actccttttc
      301 ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg tcctaaaatc aattcattac
      361 catcgaataa ataatatcct gcaaagacag tctcaggcaa agtctcattc aacaatgcag
      421 atgaatttgc taaatttgct aaccagttat cttctgcttc taataatcct tgttgctgta
      481 ataataaaat ttcgtatgca tcttttttct gtttttctgt ccacatgata aaaaccgctc
      541 ctttttgatc tatggagaag attatagctc agtttttttg taaacgctag gtttcctcct
      601 caaaaaaacg agatacgtca ggttaatcac cttgaacgca tctcgctgtc tttttctatt
      661 ctgcttgttg gatttctgct gctaaatcag cataatcaat cgtttcgtga acaaaagtcg
      721 gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg gctaatctca acttttcctt
      781 cagccacttc aaaatccatt acatgtccgc caaatgtatg tgtatcatcg ataaaatgca
      841 ggtgaaatcc cgctgcagca actccttgga ataattcagg cgtaaagaac ccgactaatg
      901 ttccttggat attgctttgt tggaattctg gttgagactc tgaaattttt gctagtcttc
      961 tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat atttttgaat aatccagtta
     1021 tcttcactgc caaaaatagg ttttccccgg cttctcttac taatatgtct tttttcaatt
     1081 gttctccatc tgcagttttt gagacagaaa aactagtatc tggcgtaaaa tctgtaattg
     1141 ctgcataggg ggtaagttca cttccatcta attgggcaac tgagccatct gatcttcctt
     1201 gataagctac gccatctaaa aagatgactt caccatcaag cccatgcaat gttcccaatc
     1261 ccagagttcc tttttctaat atatgagcaa tcgtttctgt tccatccatc aagccggcca
     1321 tcaaagctcc taatgtcccg tgctggtata agattttttc agtcatgtcg acacttcctt
     1381 aatagaattg atctggtaag attgtttctc ccaatttctc attatcacgg taatcaatcg
     1441 gaatatcaat aattacgggt ccttctgtcg caaatccttc ttctaaagct ttttctaatt
     1501 cctctacact tgtagctcgg attcctttag cgccaaaagc ttctgcatat ttcacaaaat
     1561 caactggacc aaagtctaca cctgacgccc gctgatattt cattttttct tggaactcta
     1621 ccatattgta gtgtccatca ttccaaatca aatgaacgat attcaatttt ttacgtaccg
     1681 ccgtttctaa atcctgggca gagaataaaa atcccccatc gccggaaact gatacgattt
     1741 gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt
     1801 gcataccatt actgaataac aaatgacgag gttcataact tctaaagtgg cgtgccatcc
     1861 aaatataatg actacctaca tctacggtaa cagtcatatc atctgttact tttgactgaa
     1921 gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc tccttttgat tgaacaatgt
     1981 cccgctccgt taattttgct tgaagagatg ctaaatattc ttttgcatct tctgacactt
     2041 gttgaggctc taatgaacct gtcaataaat caagagtggc tgagatatca ccgatcaatt
     2101 ctctttcagg ctgcataaaa ggatcgattt cagctggcgc ttcatcgatc acaatgatcc
     2161 gcgcatcttt ttccgcattc cagtttcttg cttcatattc gattggatca tagccaattg
     2221 caataactag atcacttcgt ttcaaaagca tatctcctgg ttggtttcta aataaaccca
     2281 cacgaccaaa gaaatgcgct tctaattcac gtgagatcac tccagctgct tggaatgtct
     2341 ctacaacagg caatgctgtc ttttcaacta attgcctgat ggctaacgtt tctttttcac
     2401 ttgatcctcg catacctact aatagtaccg gtagttttgc ttctctaatg cgttcagcta
     2461 ggtaacggat atcatctgca gaagcagaac ctaatttagg atcgctcaat ggcttgatga
     2521 cattaccttg gacaggtgca tccactacat cttgcgggat actgataaag ctcgcgcctt
     2581 tttttgaact cttagccata cgataagcat tggcaatcac ttctgaaatt gtttctggat
     2641 cttggatttc tgcactgtat tttgtgattg gctggaacag tgctgcatta tccatgcttt
     2701 gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt
     2761 caccttctgc tgtcgctgtt accaaacctg tagctaagtt actagcacca ggaccacttg
     2821 ttgcgataac tactcctggc tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag
     2881 cattttgttc gtgacgagtc acgattaatt caggaccttg atcctctaat tcattaaata
     2941 caccatcgat ttttgcacct ggtataccga atacataatc aacatgatga ttaatcaaac
     3001 tttctactac agatttcgaa ccttgtttca ttttttcagc cattgaacat cgtccttttt
     3061 atgctttatt tttaattatt agttagaaat aatcacaaat aagtgataca gtttctaaac
     3121 ctgtttcata caaaagtata gcacgtttta tttcatttgt ttagccatat gtttcaaaaa
     3181 atgaaaaaga atgacagttt ttacataaaa aaaactgtca ttcttaattg aaagtttcaa
     3241 tttataaagt ctacagatcc atagttctca tcaatttacg attgttgaag taaatcaaac
     3301 caaatacgat agaaggacct agtagaatac accattgggg ctttacgaaa aagaagagaa
     3361 acaaacttgt caatagtaaa aaatctagta aactcacata ccattttttc aacgtatagt
     3421 agaacgctat ccgtagaact tgatttggtt tcgacgctgg atttctcacc tgaaaatacc
     3481 agcagttggt gctaaaggaa actgataaac aagcaagtaa aacaaacaac ggaatcaacc
     3541 attttccata agaaaaacgt ataaaaaaga aacaatcaaa aatcattatc acactaacta
     3601 tccaagcaaa tagccaataa aaaaatccct ttagccaaaa ttggcgaaac atctttatga
     3661 acaaacgtac tggtgaaaaa ctattttctt ttgctaattc ttctgtcata cctatcatca
     3721 cgatgcaacc tggaccaata aaaagtaaag aaaaaataaa ggaaaagaca ttccttccat
     3781 ctatcgacaa aaggaacgca gctgctatat acaagatatt gacacaccaa aaacaaaggc
     3841 tacttgccaa tgattgatac aaccaataaa agaattccat ataaacattt ttgtcaaaac
     3901 gctgctcttt cataatcacc tcctattcct tcattcctga tgaagcgatc ccttctacaa
     3961 aatatttttg catcgctaaa aatataaaag caacaggaag aacagaaaca actgaggcag
     4021 ccatgattaa tgcgtactct gcatcaaata gcccaataaa cattttcaac cctaactgga
     4081 ttgttttatt ttccgtagaa gacaaataaa tgaatggccc catataatcg ttccatgtat
     4141 taacgaatgt gatgatcgtc aaactggcaa tcgccggttt tgtaagaggt aaaatgatct
     4201 tgcgatagat tccccactct gataagccat cgatccgcgc actctcacat aaagaatctg
     4261 ggatactact gtaatattgc ttcaacaaga acacaccaaa agcattgaac gcttgtaata
     4321 gcaccaacga ccacaacgta tctgttaaac ccacttttct catcataatg aactgcggga
     4381 tcatataaga ctgccaaggg acagcaatcg tgccgatata tgctaaaaat aaaatatcgc
     4441 gccctttaaa atttagttta gaaaacccgt aagcagcaaa actagaagta aacaactgaa
     4501 tcaacgtgat cacaacgctt aaaaaagctg tattcttaaa aaatgtcact aaaggaatac
     4561 gagaccaaat aaccagataa ttctctaaat gccaagtttt agggatccat tgaatcggaa
     4621 tcgtgaacac ttcattattt gctttgaatg aagcagaaac catccataaa aaaggaatga
     4681 tcgtcaataa agctaataca atcagcagaa tcatacccaa ccattgaata aagggcatgt
     4741 tttttgaacg aatttttgct ttttttcttt catttcgaac acctccaaag ttataatctt
     4801 catttctttg taatgattac aatccagaac aacggttatt atagctgatt catttccgtt
     4861 cttcttattt ttgtaagcgc ttatttaaca agttatgtaa atctctttat ttttttgatt
     4921 taaaaatgaa aaaatacaaa agcaagactg tcatacgtct cgcttttgta ttgaaatttc
     4981 ttcgattaga gatacttatt actgttcaaa agcttctttg aaacggtgaa tcaattcatt
     5041 gactacaggt gataaaatcc gattcttttt ccagactagc atgcagttcg tttcaatcat
     5101 tgggtcaagc ggaacgaatg tcactcgctt tgaatgatga cttacagcac cttctatgac
     5161 taaagcagca cctacctgat tctcaattag cgcaaataca ttgaaaatca aattgtacgt
     5221 tcctgtgatc agcaactgct ccttttttat gcctaaccag tcagcaaaca ttcgctgtat
     5281 ttctggacgc tttgagatca aaagaggtac attcgcaaag tcttcttttg ttatcacgtc
     5341 tttttttgct aaaacagaat tttctaaaat gagcaatccc catttttcct ttcttggtaa
     5401 caaaatcgtt tcgtatttct ctgtatctac tggatctagc aaaatcgcta aatctaacag
     5461 gcttttttct agtcactccg tgatatcatc acttgttccg ctgtggatat gaaattgaac
     5521 gctgggataa tcacggatca acttttctag taatagtgcc aatgtattcg aactatcagc
     5581 ttccacacag ccgatcgtaa atcggccgct gaataattgt ttttttctgt tctcgaattc
     5641 ttgagtggtt tgattcgtta aatctaaaat ttcttctgct cggctttttt agaaaataac
     5701 ctgcttctgt caagatcatt ttgcggtttt cgcgtacaaa cagttctgtt cctagttact
     5761 cttctaactc cttcagttgt cggcttaagg ttggttgtgt aagatgtaga acttctgcgg
     5821 cttgagagat tgttccttct tctgcaattg tccaaaaata ttttagtaag cgtaactcca
     5881 tagcgatgct ccttgatttt tttacttgat acattccatt ttatttctag ataacacacg
     5941 atcatatttc ttgatttttc cttgtaaacg ttccaaacat tcttgcattt cagcaatttt
     6001 tttcgtcaaa agttcacttt cttcaatcaa aagcttacgc cgctcaaata cagttgcttc
     6061 tccttcgcta gataggcgaa tatattcaat caaagactcg atcgataatc cggcatttcg
     6121 catacagatc gtaaagtcga cccattttag gtcttcttct gtataatcac ggattccgcc
     6181 ttttgtccga ttaactggtg gaatcaaccc gattcgttca taatagcgca atgtatcggc
     6241 cgaaatgccc ttttcttcac tgacttgttt gatattcatc tgatcactct ttctcttaat
     6301 ttcttgccac tttacttatg tattttattt attctacaac tttaagtgta ctccaagtca
     6361 atactcattt ctattaaaac taagctggat tatctatatc ggttgtttgt gtattatttc
     6421 aaataaaaaa gtcttgtttt tttattcttt tttggatata cttttaacaa tttagggaaa
     6481 ttttaggaaa aggggtataa aaatgacaac gtatcagcga aaggtattag tatcgacatc
     6541 ggcgggaatt gctttagaga atatggatat tatgttttta gctttttcat tatcttcaat
     6601 gattacttct tttcatatta gtggtacgca agcgggtttc atttcaacga tcactaattt
     6661 ggggatgctc attggaggta ttttttttgg catcatagct gataaatatg gacgtgtgaa
     6721 agtattttct caaacggtcc ttctgttttc gattgcttct ttattgatgt attttgcttc
     6781 aaacatctac ttggtttatc tctttcgctt tatcgcaggt gtaggagcgg gaggagaata
     6841 tggggcgtgt atgtccttga tttccgaaag tttttcaaaa aaacaaattg gacgagcttc
     6901 ttcggttgct ggtattggtg cgcaggtcgg tgcggctttg gctgcgattt tagctgctgt
     6961 gatcattcca acgtttggtt ggaagatgtt gtatgtcgta ggtgtactgc cggtgttgat
     7021 ggtcttgttc attcgtcgcg gcttgactga accaaaggaa ttcaaatctg tacaatctgc
     7081 cgaaaagaaa accaaactta gcagactgtt tgaatcaaaa catcttgctt ggcaaacatg
     7141 tggccttagt atcatggtaa ctgtccaaat tgccggctat tttgggttaa tgaattggct
     7201 accttcaatt atgcaaaatc gattagggct ttcggtttct gggtcttcat tgtggatggt
     7261 cagtactatt ttaggcatgt ctttggggat gatgactttt ggcataatca tggataaatt
     7321 ggggcctaaa ctagcctttt ctttgttttt aatttgttca tccttatctg tctatctact
     7381 tgtgttagcc catagccagt ggagtttggt agcagccgct gtgatagtcg gctactttat
     7441 caatggcatg tatggtggat atggcgcagt gattagtagt ttgtatccaa cagaaatccg
     7501 cgcgacggcg aataatttcg ttatgaacct tggacgagct gttggtggtt tttcttctat
     7561 cgtcatcggt ttcttgatgg atcattatag tttaactgcc gtaatcttgt ttttgagcgg
     7621 tatttaccta gtcagtctgc tggtcttgac aactcttact ggtgttacag cattaaggga
     7681 aagcttgcag cattgatagt tgagtctgta aatgacaaga aagaggtgtg gaaaaagctt
     7741 gcatctgcgg taataagacc agccaaatca aaaacatgag acactgtttc cgtttggctg
     7801 gtcgtattac ttgatgcagg gcagcttttt caactgctaa tttccttcta tctggcggcg
     7861 actattcttt gacgtccgtc atttcaaagt actttactaa taacttagca taacgaaaaa
     7921 ctgtggatac actcgtatat tgagcgtctg cataatcttc caaatttttc tcaggatgtt
     7981 gatacatgaa ttcaatcaaa tgataattat atgattttct gtagaaatga agataaataa
     8041 tgtagattgg gaagttctcc tgcttttcca tagaaaagcg ccgattattc tcattcttca
     8101 aaatgacctt atcaggatat tgttggaaca attcttctaa atctgttaga taattcaaga
     8161 tagacttact tgataccttc aattcgtttt gtagttcttc tattgaacac caacgttctg
     8221 tttcaagcag taaatgaatc attttaattt tcttttgaga aacttttgtt aaaaaagtct
     8281 gcatattttc tcctcctgta tctactttac tatactacat acaagaaaaa aacggataca
     8341 aatatcatat tgtcacaccg tcaatagtgc ataaacctgt tattatttct ctttttttcc
     8401 taagtattct attgatatga atcatttaat cgtttattat ataaatgtca ataatctaat
     8461 aataaaataa cgtacatatt ttattattgc atacttaata acagaactat agcaaaaaat
     8521 cccgtgtatc aacacgggat tagattaagt ttcctcaatc actattcata acacaattat
     8581 tataacaagt aagtaaacaa ttgaaaagga catttattga aaacggttgt tttttactta
     8641 aggaggaaat ttagtgaaaa aattagttgc gttgtttttg gtcgcaggaa gtttgttgag
     8701 tgggtgtggt gctactacgt ctactactca aagcacaagt tcagctcagg cttccagcag
     8761 cagtaccacg atctccgaaa gtacggcaga ttcatcgaaa gaacaaattg aaaagaaaaa
     8821 agaagaagag aaacaaaaag cggaaaaagc tgcactagaa aaaaaagtga cagaagcgga
     8881 atctgctttg tcacaagctg aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc
     8941 agctatccaa gcgctacctg aaaacaaaca agagttgaca agccgtcttg ctgcaattga
     9001 ttccacaatc aaagcaaaag aagcagaaga agaacggaaa aagcaagaag aaattgcacg
     9061 acaacaagca gaacaagcgc aacaagaagc aatcgcacag caacaagcag aacaggcgag
     9121 gcaagaagca atcgcacagc aacaagcaga acaggcgcga cagcaagcag cagctgaaca
     9181 ggcttctcag caggcacaag ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt
     9241 cacaccgacc ggttcaaaat accatacaca taaatgtgga aatggtacgt atacccctgc
     9301 cactttaagc gaagctcaag ctcgtggttt aaccccttgc tccaaatgtt atggaaacta
     9361 attcaaaagt tatactataa taataaaatt cttctgaaac ataaaaaaat agaagctggc
     9421 gttccagata gtaaaccaaa aatagcagtt tacacgtcca tttcgtcagc ttctctcttt
     9481 tttcatttag tctgttccct ctgctttttt ggacatcttc attcattctt tctcaatcaa
     9541 atagtacacc tctttttctt ttcgcagttt atatcttcct tttttcaatt tcattgaaaa
     9601 ctgtttcgaa tgtttatgtt ttacatggat tttaagcagg agctcctcat gttgcaagcg
     9661 atagttcctg ccttttaaat cagaaaatct cactagtccg tattcatcag aagctttcac
     9721 aaatggaact ttttctctag taatatagtg tttgccgttg agcgttttta cagaaaagac
     9781 cattccttcg attggtcgtc cgtttgtgtt ctttacataa aaagataaag gatagtttcg
     9841 cttagcgatg gccaaagaat ctaaataagc gatgagtagc caaactaagc cgattgatcc
     9901 aagtagtata ccgataaacc aaataaaaaa tttccatttt ttccattgat caattccttg
     9961 gatcgctttt tttgcctctt ttgcttgata aggaatacga tgccccgtca caagcagtcg
    10021 atgtgtattg accatataag gagtgcaggt aagcaaagtc acgagatctc tgcctttttg
    10081 gatacccaaa gaatctactt cgtcaggtaa gaccacttgg attttttcta cttgatacgc
    10141 caatgtcttt ccattgatct ggatgaaaaa ctggtctcct tttttcaact taggcaaatc
    10201 tgtaaataat tttgcttctg ggagtccacg atgcccagaa agaacggcat gggtgttttt
    10261 cccgccagtc ggataagaac ttccttccaa taatgacgtt cctttttgca aaaatatttc
    10321 cgttgtctga tcgaatatag gtaagctgac attgattttt gggatttcga tgactccaag
    10381 catatgttcc ataaaaaagg cattactagg taaatctttt aatgcttttg catcaactgc
    10441 ttggttgaaa ctgcctatcc ctggtgatac gttttgctct gccaactgtt ggtttttctt
    10501 tgtcattttt tcctgctgtt tttttatttc ctctgaattc ttattcgatg cttgcttttg
    10561 ataatggatg atcatttgct gggcaagaaa atcattcagt gaatcttcta caaaaggata
    10621 agaaaagatc ccgatcccaa cgactaaaat gattgccatg aagatgtcca gtattcttat
    10681 tctgctttta ttcatcatgt ccacctattt tctaatatgt atatgaagag gttgtgaaat
    10741 cagcgccttg acctgagtat tagcgaagca aacggcaaaa tagttcctcc tattttgccg
    10801 tttgttgagc gaataccaag agaccgacca cttgtcacaa cctttctccc catttgaatc
    10861 actatcgttt atttatcgta tgatttttgt ttccaatagt ttaagcttct gtttcttgat
    10921 ggcgtttttt gaaatagatc actgctcctc caactaaagc agtacctacc aagatgaata
    10981 cgatgatccc cataccacca gttgaaggca agattccttt aggttggtta tcgatcgttg
    11041 tttcatcaga agttgttgga tcatcatcta ctgtaaattc agtgtacgca gcttcattct
    11101 taggtaatgc gtagccttct ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca
    11161 gcccttcgat tgaaaatttc ccttctgaat cagaaatcaa tttcacttct tcccagtcag
    11221 aacctgtgac ttcatctgac caagcatatg ggtattctcc tgaggcaagg tttgctattt
    11281 ttttcattaa tttaacagtt ggatcttttg ctcttagaat agaaaattct gcatcggcta
    11341 atttttcttg cgtgttcaca tcttgtttga tgaagtggaa atcaccgtag ttttggacat
    11401 tttctacagt aatcgtgtta tccgtagttg tattttcccg attgactaca ccgtttggat
    11461 tcccaagatt ggcttcatct gcgccatgga tgataccggt tgtttgttgc aattttccgt
    11521 tttttactat aaaagtagtt tctttttgtg gattggttaa ttgataacca tctggtgctt
    11581 ttgtttcaac taaagtataa ttgccgtcta ttaatccatt gacactgatg gatccttcag
    11641 tatcagttgt gaatgtcaac gctgactcag caggatcata gagagcgaca ccagtgtttt
    11701 catcaaaacc ggtcacgaat tgattctctg aatttttgat ctggaattgc gcccctgcta
    11761 acttatctac ccctgaaaag ttcccagctt tctggatttt gatattgctt tctttgatga
    11821 catttttagc atataggtga atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa
    11881 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
    11941 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
    12001 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
    12061 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
    12121 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
    12181 attaaccgta acattttctg gtgtaggtga tacggctgca gaaacctctg cagttcctag
    12241 gccgagaacg aaaggaagca gcatgatagc agttcctaaa aacgtccata tttttgtttt
    12301 gatgtttttc attttatttc cctcctattg tttccttttt gttgtttttt ataaaaccaa
    12361 gctgtccctg ttgaaaggac tagcaataac cctaaaattg agaaacctcc aagcatctct
    12421 tcgcttgttt ttggaaagct tcctccattt ttttcatttg atggaccatt tgtctgcatc
    12481 tgaactggcg tttttccatt attcgtcgga tttgcttttg gttcgttgat gatttccaat
    12541 ggtataggat gaagcggatc ataactgcct ttttcaactt caaagagaat ggttgattga
    12601 ctcaatatat atcctttagg cggtttaatc tccactagtt cgtaacttcc aaaagacaat
    12661 ccatggacag aaaatgttcc atctttactg gaagtaaaaa tcgttgctct tgaatcatct
    12721 ttggctaccc aatgatttat cccgttttct tgcgctaagt attggtcatt ttttttgact
    12781 agaaattgag catctgctag tttttgtttc gtttgccgat ccactttgac aaattgtttg
    12841 cctcctgttt ggatggcaag atgtgtttct actgtttgat tgtcagtcgt caaacgtaca
    12901 tcgttatcaa atgtcgtttt ttcactgttt tctttcaatg tcatttggta acggatcgtt
    12961 aatttttttc cagcaaacga actaagatct tttccagaat ctgataagac aaaaccatgt
    13021 tttatttttt gtgtgtgaaa tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt
    13081 tctggttcta gccataactg aggatcagct gtatctttga tcgtataggt tcctatctcg
    13141 ttgatgttct caggaattgt tacggtgatg agataaggga ctttatcccc atctgcaaaa
    13201 tctgtctgct tattctctac tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg
    13261 ctctttggat acaagtggat ggtcgtcaat ttatgattag atgaatctgt cacaggaagc
    13321 acaacgacaa atggtgctga tttttctttt acttaatctg aagtctttat ttccgtaaat
    13381 cggtaaacag cgtcacgacc ttgctcatct ttgtccgatg cactaaaaga agcaatgccc
    13441 tcttcctcgt ctactgtttt tgtcacattt tctgccagta ttttttcact atcacttttt
    13501 tgtgctagtt ttctttgtgc gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat
    13561 tcatccgaga catcatatat agcgaaagta acgccgttca acccttttac tccttgaaag
    13621 ttatccggga tatttattcc gtcgttttga ctgatacttg gcatcttatc gctttcgaac
    13681 attcgattat gcaaagtaaa tgtaataggt gattctctag ctgacacagt cggagaacct
    13741 ataaaaaaga ccagtaggat aaaaaagaga acagcaaata tatccacacg tacatttttt
    13801 gtttttttca tcgctgttca cctgcttttt tcttacgatg gataaaataa aagaagccag
    13861 aaaaaataaa gatcagtcct aaaaagacaa agggcagtag acctatccca cctgtcgaag
    13921 gaagcaagag agtcggattg atcgtcaact tagcggtatc tgttgcgata atcgtataat
    13981 tattgtttac acttccttcg cctaaactaa tcgttactat ttgtgatcca atatctgtcc
    14041 ggctgttaac tgttagggta taatccaatg attcgcctaa gacaacaccg gtaacgtgga
    14101 gatttccaac cgaaatcgag tgactttggg catcataatt aaaggcagcg tcatcgacag
    14161 taatcactgc gttagcttta tagatggtat aagttcctga tgtgttggct atatcagtaa
    14221 atgacaactt gggatttgct gctttgactt tgtctattcc gctattcgtt agtttaattg
    14281 tatagctgcc agcattaacc atattttttg ctgattgtgc atctgctgtg taatccatac
    14341 tgctaagtgc cacttgtact ggagtaagcg aactagtgac accctctgct agcttgacat
    14401 ggattgacgg gacacctgct tttccatcat atgtcttact gccactgagc tctaccgcac
    14461 cgctgatttg cttaatcgta tagacatcag atgtctgcac gatagtaaat aaatagtttg
    14521 ttctggcagc tagtttagct agtccgcttt ttgtcaattg ccaagacgta taggttccaa
    14581 tttcagtagg cgcagttact tcaactccct tttcattcat cgctttgaaa tcagttggat
    14641 tccatgtgat ttgatcaata ctgagatcat tgtttaaaag ggatgttgat tctgttttat
    14701 cattaaaggc atatttaacg atcggtacaa cagaaagacc atcatagtac ttactgccat
    14761 ttgcattgat tgatactgtt gcatttgtct tataatatag gatgacttca gaaatctgtt
    14821 ttccatttgc gtcctttacg attacatttc catttgtatc taaactagct aaaacactca
    14881 ttgctgattg gttctttgtt tctatcgctg aaacagctgt aaaggtatat ccgttgatag
    14941 taggcgtatt agtcgtcagg tcttgtgcag cacttccgta agtaagattc attattttag
    15001 aaggagcaat catctgtcca aaatagtctt gatacttaat agtgattggt gtttgtttgc
    15061 ctgtataggt caccgtatcg acgttacttt ctagggctgt gaaagaaccg tttgtttgtg
    15121 caaaagaata gcccgttact gctgttggag tgataaccac tttgtcaccc acatggattc
    15181 cagataatgt agtcccgtac gttccgattt ttgctgctcc accattgact gtataagaaa
    15241 aagttcctgt ccgattcgca ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg
    15301 aaacatttcc cttaccatca acgatctgcc catttttgat cgagtacccc gtgaatggaa
    15361 tatcatttgc taactgtata ccgattgaaa aagtactcat acctcctccg gcaggaagcc
    15421 ctccccatgt tgagggtgta aaagaataac ttttatccgc attgggtgta actgaatcga
    15481 tcgtatttcc agcggtagcc acccccatat agatactttt tgggaatgtg atagtagtac
    15541 catttactgt ggagccaata aacagattat ggatatctgg acggttatca ataaaagcga
    15601 ttgctttggt tacgccttgt aacgcagttt tatccgcact catcgatccg ttaaccgcaa
    15661 caatgactcc atctttggca tatatcactg tatatccatc tggtatggta tgtccgttga
    15721 aattcgcagt ggccgcaaca gtactaaatg tctggcttct acctgtttct gaataagctt
    15781 tctcccctgc tgcttttgct gctgtgattt ctttttctgt cggatcaatc agttgagtcg
    15841 aggatgtggt tgaggttgtc tcgttgttgc tagactgatt ggtgctttct atggactgta
    15901 tttcagaata gggtggacta tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt
    15961 ctgtataaac gtttacataa atacttgata agctccctaa gaaagtaaaa atcaagatga
    16021 tcgaaatcat aatgggatgt aagtattttc ttttagatac agctttgagt ctagaaacaa
    16081 aatgtttttg acatatacgt tggattttat tttgagtttt cttaatcatt ttcatccctc
    16141 ctctcaataa aaaatcatca aataatcctt tacacgttat tctaaattt
//
LOCUS       DARBER010000046        16163 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARBER010000046 DARBER010000000
VERSION     DARBER010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.046"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="SCK06_001458"
     CDS             complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="SCK06_001458"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG1151464.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(1390..3468)
                     /gene="topA"
                     /locus_tag="SCK06_001459"
     CDS             complement(1390..3468)
                     /gene="topA"
                     /locus_tag="SCK06_001459"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG1151465.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="SCK06_001460"
     CDS             complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="SCK06_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG1151466.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(4503..5270)
                     /locus_tag="SCK06_001461"
     CDS             complement(4503..5270)
                     /locus_tag="SCK06_001461"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG1151467.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="SCK06_001462"
     CDS             complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="SCK06_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG1151468.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            6383..6781
                     /locus_tag="SCK06_001463"
     CDS             6383..6781
                     /locus_tag="SCK06_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1151469.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            7060..7560
                     /locus_tag="SCK06_001464"
     CDS             7060..7560
                     /locus_tag="SCK06_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1151470.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="SCK06_001465"
     CDS             complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="SCK06_001465"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1151471.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8346..8573)
                     /locus_tag="SCK06_001466"
     CDS             complement(8346..8573)
                     /locus_tag="SCK06_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG1151472.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="SCK06_001467"
     CDS             complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="SCK06_001467"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1151473.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(9108..9821)
                     /locus_tag="SCK06_001468"
     CDS             complement(9108..9821)
                     /locus_tag="SCK06_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG1151474.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(9824..10720)
                     /locus_tag="SCK06_001469"
     CDS             complement(9824..10720)
                     /locus_tag="SCK06_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1151475.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(10878..11720)
                     /locus_tag="SCK06_001470"
     CDS             complement(10878..11720)
                     /locus_tag="SCK06_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1151476.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11852..12505
                     /locus_tag="SCK06_001471"
     CDS             11852..12505
                     /locus_tag="SCK06_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG1151477.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(12568..13095)
                     /locus_tag="SCK06_001472"
     CDS             complement(12568..13095)
                     /locus_tag="SCK06_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1151478.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(13115..14062)
                     /locus_tag="SCK06_001473"
     CDS             complement(13115..14062)
                     /locus_tag="SCK06_001473"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG1151479.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(14074..15960)
                     /locus_tag="SCK06_001474"
     CDS             complement(14074..15960)
                     /locus_tag="SCK06_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1151480.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         4256 a         3340 c         2826 g         5741 t
ORIGIN      
        1 aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt
       61 gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt
      121 gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag
      181 ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt
      241 ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg
      301 ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc
      361 tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct
      421 ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct
      481 aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag
      541 cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa
      601 ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc
      661 gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct
      721 ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg
      781 aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac
      841 ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca
      901 tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa
      961 gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt
     1021 atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg
     1081 cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga agtgattact
     1141 accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt
     1201 cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc
     1261 gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa
     1321 gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc
     1381 tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt
     1441 accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac
     1501 tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct
     1561 gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc
     1621 gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa
     1681 cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc
     1741 tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt
     1801 ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat
     1861 tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa
     1921 ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt
     1981 caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg
     2041 ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg
     2101 ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag
     2161 ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg
     2221 catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc
     2281 taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga
     2341 ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg
     2401 tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt
     2461 tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc
     2521 tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat
     2581 caagccgact gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat
     2641 catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt
     2701 agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa
     2761 gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt
     2821 tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt
     2881 accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat
     2941 aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg
     3001 ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg
     3061 tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc
     3121 ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta
     3181 cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt
     3241 ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga
     3301 aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc
     3361 tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt
     3421 cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac
     3481 tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca
     3541 tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa
     3601 attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat
     3661 gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa
     3721 gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac
     3781 atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg
     3841 ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca
     3901 agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc
     3961 gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac
     4021 tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg
     4081 ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata
     4141 gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat
     4201 atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt
     4261 gaatttcctg ccaacttttg cgaaacagct cccgatgttt ggttatttta cctatcttaa
     4321 tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc
     4381 attttccttg actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt
     4441 gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt
     4501 ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct
     4561 tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca
     4621 tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg
     4681 atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga
     4741 ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt
     4801 tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc
     4861 tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga
     4921 agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt
     4981 ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca
     5041 tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt
     5101 tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt
     5161 gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt
     5221 ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg
     5281 ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg
     5341 cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc
     5401 catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct
     5461 gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa
     5521 atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc
     5581 ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg
     5641 aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc
     5701 tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg
     5761 aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc
     5821 tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc
     5881 aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag
     5941 atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg
     6001 attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt
     6061 cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt
     6121 cattgttctc tccattcctt tcataccgga atattccccg gctttacttg tttttttctt
     6181 tttcaatata aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt
     6241 gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt
     6301 ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca
     6361 aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga
     6421 tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc
     6481 tactgaagaa gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca
     6541 tgatttttat aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg
     6601 tgctttgatt tcgtttagtg ttgaaaacgc ggatgaagta aaaaaatttg ctcaaattgc
     6661 aaaagaaaat ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt
     6721 ttcattagaa gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta
     6781 agcagataac taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag
     6841 tattctccgc ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag
     6901 tcttctcacc ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg
     6961 acacattggt gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg
     7021 aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg
     7081 acctctgctc ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct
     7141 tattttcaaa tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg
     7201 atctgggctg gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca
     7261 ggaacgtttc aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta
     7321 tttttatatc ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg
     7381 atcccagtca gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc
     7441 aaagtaattt ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg
     7501 gttgtttatt catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga
     7561 ttttacgtac taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac
     7621 agactgcacg ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga
     7681 caaatcttgc tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt
     7741 ttgaaactct tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc
     7801 ccgttagctc ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca
     7861 ggtttttagt tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt
     7921 ggtagctgac attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg
     7981 ctaattgaaa aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca
     8041 tatcaccttg cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata
     8101 aaaatccccg caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc
     8161 agaagcgatt aatatagcgt tgtttttttg tcaatctcta tcaatccatc cctctttttt
     8221 tcattctttc tttattataa gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa
     8281 aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact
     8341 atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc
     8401 atggacggca aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag
     8461 ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca
     8521 tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt
     8581 tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag
     8641 gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt
     8701 cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt
     8761 tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac
     8821 cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta
     8881 ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc
     8941 cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc
     9001 ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaagc
     9061 agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat
     9121 gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt
     9181 gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca
     9241 tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc
     9301 aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag
     9361 tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga
     9421 cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag
     9481 aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa
     9541 taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt
     9601 tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta
     9661 agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat
     9721 acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt
     9781 ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct
     9841 tctctaacca ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt
     9901 gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc
     9961 catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt
    10021 cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt
    10081 tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga
    10141 tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt
    10201 tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg
    10261 gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat
    10321 cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt
    10381 cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt
    10441 ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga
    10501 atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt
    10561 agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg
    10621 ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg
    10681 ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct cttgtattcg
    10741 attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac
    10801 aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg
    10861 gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta
    10921 tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct
    10981 tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt
    11041 tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac
    11101 gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc
    11161 acgcgtatgt tcaaaacact agacagcaaa ccagttgttc ggctgattct tccacctttt
    11221 accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg
    11281 acttcttcaa ggattttctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt
    11341 acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt
    11401 gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg
    11461 atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat gaattctcca
    11521 attggtggtt ggcttgtttt gggcagattt tttgctttcg ccatcatttc cataaatctt
    11581 tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc
    11641 atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt
    11701 acgattttta catttgtcat tattttctct ctctctttct ttaacaagat gccttattca
    11761 tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc
    11821 attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta
    11881 tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat
    11941 cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt
    12001 ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca
    12061 ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt
    12121 tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg
    12181 ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt
    12241 gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg
    12301 gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat
    12361 tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc
    12421 tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta
    12481 cttctctgtt ttattcttta cttaataaaa aaaataaagc tatggatggg acagctctaa
    12541 ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc cgctttttac
    12601 aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt tttcatccat
    12661 ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg gaaaataagc
    12721 atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa atggcagaaa
    12781 ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt ctttttccag
    12841 agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat aggaagaatc
    12901 tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc cttcgaatgt
    12961 ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt
    13021 tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat cctgtgccca
    13081 aattgcagct aacattttct catcctttct gattctaaac agcgatagga gctttgatcg
    13141 ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca aatactgact
    13201 gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc aattgttctt
    13261 tcatttgttc gatatgattg atataaagat gagcatctcc taatgtgtga acaaattcgc
    13321 caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt
    13381 tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag cttagccgtc
    13441 cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg ctaggaacat
    13501 cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt ttgattgctt
    13561 cgatcacttg tttgatttga tcgataaagc caccgtcttt tgtttcccag tgacgccatt
    13621 gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca tcggttagga
    13681 tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca ggatctgtca
    13741 aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt ttgatgtagc
    13801 gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag cggatattcg
    13861 tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg
    13921 ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg aataagctta
    13981 atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat tttcttccta
    14041 agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt cactcaagta
    14101 ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa
    14161 ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga gcaatgagat
    14221 tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt gttccatgta
    14281 agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg ctttttcagc
    14341 aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt aataaagttc
    14401 ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca agaaataacg
    14461 gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt ccaaaatcgt
    14521 caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat taggttgctg
    14581 aatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt tgcctatcag
    14641 tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta ctccgatttg
    14701 actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa tcatccgttt
    14761 atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg tttcaccgat
    14821 agacattgta ccgctatcca ggggaattct gccagctaga atattaagca atgtcgattt
    14881 tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca ataaatctaa
    14941 atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt ccagtacttt
    15001 tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt ttacttggta
    15061 caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc
    15121 ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc gcttttcttc
    15181 ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt cataatttcc
    15241 tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta ctctatctag
    15301 aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca aatagttttc
    15361 caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta atagtaaatc
    15421 gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac ctgataaagt
    15481 actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct ttgcgttggt
    15541 atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat aacgtttttg
    15601 cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat atgctcgaac
    15661 agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta attcatcgga
    15721 aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa acactgtttg
    15781 ccgatcaccg tcgctgctat cgataccggc aataatattt aagagacttg ttttacccgt
    15841 cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag aaatctggtc
    15901 aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt ctttcaacat
    15961 gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga aaattttttt
    16021 ggtatttgtt ctttttgtgt tctcaaaaaa agaacaggac cgattcgatc ctgttctgat
    16081 atgttttgtt ttttataaaa cttgaaaatt tttacctttt tattttattg gactcgtttt
    16141 tttgactttg ctcgaaacgc aaa
//
LOCUS       DARBER010000047        16159 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARBER010000047 DARBER010000000
VERSION     DARBER010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.047"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            21..1448
                     /locus_tag="SCK06_001475"
     CDS             21..1448
                     /locus_tag="SCK06_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1151481.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="SCK06_001476"
     CDS             complement(1540..1905)
                     /locus_tag="SCK06_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1151482.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="SCK06_001477"
     CDS             complement(1902..2267)
                     /locus_tag="SCK06_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1151483.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="SCK06_001478"
     CDS             complement(2459..2707)
                     /locus_tag="SCK06_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151484.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="SCK06_001479"
     CDS             2706..2834
                     /locus_tag="SCK06_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG1151485.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="SCK06_001480"
     CDS             2891..4585
                     /locus_tag="SCK06_001480"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG1151486.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4589..4708
                     /locus_tag="SCK06_001481"
                     /pseudo
     CDS             4589..4708
                     /locus_tag="SCK06_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4685..4858
                     /locus_tag="SCK06_001482"
     CDS             4685..4858
                     /locus_tag="SCK06_001482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151487.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="SCK06_001483"
     CDS             5236..6369
                     /locus_tag="SCK06_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG1151488.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="SCK06_001484"
     CDS             6400..6720
                     /locus_tag="SCK06_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG1151489.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="SCK06_001485"
     CDS             complement(6749..7213)
                     /locus_tag="SCK06_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151490.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7345..8598)
                     /locus_tag="SCK06_001486"
     CDS             complement(7345..8598)
                     /locus_tag="SCK06_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1151491.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8627..8944)
                     /locus_tag="SCK06_001487"
     CDS             complement(8627..8944)
                     /locus_tag="SCK06_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1151492.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8970..9290)
                     /locus_tag="SCK06_001488"
     CDS             complement(8970..9290)
                     /locus_tag="SCK06_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1151493.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9754..11169)
                     /locus_tag="SCK06_001489"
     CDS             complement(9754..11169)
                     /locus_tag="SCK06_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1151494.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11186..12502)
                     /locus_tag="SCK06_001490"
     CDS             complement(11186..12502)
                     /locus_tag="SCK06_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1151495.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12652..13350)
                     /locus_tag="SCK06_001491"
     CDS             complement(12652..13350)
                     /locus_tag="SCK06_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG1151496.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13639..14595)
                     /locus_tag="SCK06_001492"
     CDS             complement(13639..14595)
                     /locus_tag="SCK06_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1151497.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="SCK06_001493"
     CDS             complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="SCK06_001493"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1151498.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15716..>16159)
                     /locus_tag="SCK06_001494"
     CDS             complement(15716..>16159)
                     /locus_tag="SCK06_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151499.1"
                     /translation="QPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEA
                     KIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGL
                     VQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5019 a         3266 c         2752 g         5122 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gcttactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
     7321 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
     7381 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
     7441 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
     7501 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
     7561 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
     7621 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
     7681 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     7741 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     7801 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     7861 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     7921 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     7981 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     8041 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     8101 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     8161 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     8221 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     8281 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     8341 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     8401 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     8461 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     8521 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     8581 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     8641 gacgatacag attaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     8701 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     8761 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     8821 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     8881 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     8941 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     9001 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     9061 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     9121 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     9181 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     9241 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     9301 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     9361 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     9421 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     9481 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
     9541 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
     9601 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
     9661 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
     9721 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
     9781 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
     9841 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
     9901 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
     9961 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    10021 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    10081 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    10141 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    10201 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    10261 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    10321 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    10381 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    10441 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    10501 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    10561 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    10621 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    10681 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    10741 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    10801 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    10861 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    10921 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    10981 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    11041 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    11101 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    11161 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    11221 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    11281 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    11341 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    11401 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    11461 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    11521 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    11581 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    11641 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    11701 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    11761 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    11821 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    11881 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    11941 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    12001 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    12061 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    12121 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    12181 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    12241 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    12301 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    12361 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    12421 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    12481 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    12541 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    12601 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    12661 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    12721 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    12781 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    12841 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    12901 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    12961 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    13021 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    13081 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    13141 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    13201 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    13261 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    13321 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    13381 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    13441 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    13501 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    13561 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    13621 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    13681 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    13741 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    13801 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    13861 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    13921 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    13981 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    14041 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    14101 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    14161 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    14221 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    14281 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    14341 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    14401 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    14461 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    14521 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    14581 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    14641 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    14701 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    14761 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    14821 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    14881 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    14941 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    15001 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    15061 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    15121 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    15181 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    15241 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    15301 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    15361 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    15421 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    15481 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    15541 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    15601 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    15661 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    15721 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    15781 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    15841 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    15901 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
    15961 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
    16021 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
    16081 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
    16141 caacaatatg tcccggctg
//
LOCUS       DARBER010000048        16148 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DARBER010000048 DARBER010000000
VERSION     DARBER010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.048"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            226..894
                     /locus_tag="SCK06_001495"
     CDS             226..894
                     /locus_tag="SCK06_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG1151500.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="SCK06_001496"
     CDS             complement(1094..1480)
                     /locus_tag="SCK06_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151501.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="SCK06_001497"
     CDS             complement(1452..2834)
                     /locus_tag="SCK06_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG1151502.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="SCK06_001498"
     CDS             complement(2990..4177)
                     /locus_tag="SCK06_001498"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG1151503.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="SCK06_001499"
     CDS             complement(4184..5215)
                     /locus_tag="SCK06_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1151504.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="SCK06_001500"
     CDS             complement(5231..6685)
                     /locus_tag="SCK06_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1151505.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="SCK06_001501"
     CDS             6860..7765
                     /locus_tag="SCK06_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1151506.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="SCK06_001502"
     CDS             7856..8533
                     /locus_tag="SCK06_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1151507.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="SCK06_001503"
     CDS             8530..8700
                     /locus_tag="SCK06_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151508.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="SCK06_001504"
     CDS             8814..9710
                     /locus_tag="SCK06_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1151509.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="SCK06_001505"
     CDS             complement(9765..11135)
                     /locus_tag="SCK06_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1151510.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="SCK06_001506"
     CDS             11373..12119
                     /locus_tag="SCK06_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1151511.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="SCK06_001507"
     CDS             12116..12955
                     /locus_tag="SCK06_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1151512.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="SCK06_001508"
     CDS             12956..13906
                     /locus_tag="SCK06_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1151513.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="SCK06_001509"
     CDS             14047..14799
                     /locus_tag="SCK06_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151514.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="SCK06_001510"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="SCK06_001510"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG1151515.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5239 a         3015 c         3241 g         4653 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaa
//
LOCUS       DARBER010000049        16090 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARBER010000049 DARBER010000000
VERSION     DARBER010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.049"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            10..867
                     /locus_tag="SCK06_001511"
     CDS             10..867
                     /locus_tag="SCK06_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG1151516.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            982..1902
                     /gene="pstC"
                     /locus_tag="SCK06_001512"
     CDS             982..1902
                     /gene="pstC"
                     /locus_tag="SCK06_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG1151517.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1902..2786
                     /gene="pstA"
                     /locus_tag="SCK06_001513"
     CDS             1902..2786
                     /gene="pstA"
                     /locus_tag="SCK06_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG1151518.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2801..3607
                     /gene="pstB"
                     /locus_tag="SCK06_001514"
     CDS             2801..3607
                     /gene="pstB"
                     /locus_tag="SCK06_001514"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1151519.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3623..4381
                     /locus_tag="SCK06_001515"
     CDS             3623..4381
                     /locus_tag="SCK06_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1151520.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4394..5071
                     /gene="phoU"
                     /locus_tag="SCK06_001516"
     CDS             4394..5071
                     /gene="phoU"
                     /locus_tag="SCK06_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG1151521.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5309..6340
                     /locus_tag="SCK06_001517"
     CDS             5309..6340
                     /locus_tag="SCK06_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1151522.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6337..7104
                     /locus_tag="SCK06_001518"
     CDS             6337..7104
                     /locus_tag="SCK06_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1151523.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7204..8772
                     /gene="liaX"
                     /locus_tag="SCK06_001519"
     CDS             7204..8772
                     /gene="liaX"
                     /locus_tag="SCK06_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG1151524.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9069..9389
                     /locus_tag="SCK06_001520"
     CDS             9069..9389
                     /locus_tag="SCK06_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG1151525.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9389..9739
                     /locus_tag="SCK06_001521"
     CDS             9389..9739
                     /locus_tag="SCK06_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG1151526.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9906..10844
                     /locus_tag="SCK06_001522"
     CDS             9906..10844
                     /locus_tag="SCK06_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG1151527.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10859..11689
                     /locus_tag="SCK06_001523"
     CDS             10859..11689
                     /locus_tag="SCK06_001523"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG1151528.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11733..12758
                     /locus_tag="SCK06_001524"
     CDS             11733..12758
                     /locus_tag="SCK06_001524"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1151529.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12773..13711
                     /gene="galU"
                     /locus_tag="SCK06_001525"
     CDS             12773..13711
                     /gene="galU"
                     /locus_tag="SCK06_001525"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1151530.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13836..14276
                     /locus_tag="SCK06_001526"
     CDS             13836..14276
                     /locus_tag="SCK06_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG1151531.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14279..14881
                     /locus_tag="SCK06_001527"
     CDS             14279..14881
                     /locus_tag="SCK06_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1151532.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14940..16046)
                     /locus_tag="SCK06_001528"
     CDS             complement(14940..16046)
                     /locus_tag="SCK06_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1151533.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT         5205 a         2811 c         3486 g         4588 t
ORIGIN      
        1 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
       61 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
      121 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
      181 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
      241 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
      301 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
      361 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
      421 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
      481 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
      541 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
      601 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
      661 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
      721 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
      781 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
      841 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
      901 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
      961 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
     1021 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
     1081 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
     1141 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
     1201 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
     1261 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
     1321 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
     1381 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
     1441 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
     1501 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
     1561 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
     1621 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
     1681 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
     1741 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
     1801 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
     1861 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
     1921 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
     1981 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
     2041 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
     2101 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
     2161 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
     2221 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
     2281 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
     2341 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
     2401 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
     2461 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
     2521 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
     2581 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
     2641 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
     2701 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
     2761 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
     2821 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
     2881 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
     2941 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
     3001 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
     3061 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
     3121 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
     3181 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
     3241 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
     3301 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
     3361 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
     3421 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
     3481 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
     3541 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
     3601 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
     3661 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
     3721 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
     3781 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
     3841 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
     3901 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
     3961 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
     4021 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
     4081 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
     4141 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
     4201 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
     4261 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
     4321 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
     4381 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
     4441 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
     4501 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
     4561 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
     4621 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
     4681 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
     4741 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
     4801 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
     4861 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
     4921 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
     4981 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
     5041 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
     5101 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
     5161 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
     5221 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
     5281 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
     5341 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
     5401 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
     5461 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
     5521 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
     5581 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
     5641 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
     5701 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
     5761 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
     5821 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
     5881 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
     5941 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
     6001 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
     6061 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
     6121 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
     6181 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
     6241 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
     6301 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
     6361 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
     6421 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
     6481 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
     6541 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
     6601 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
     6661 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
     6721 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
     6781 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
     6841 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
     6901 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
     6961 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
     7021 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
     7081 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
     7141 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
     7201 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
     7261 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
     7321 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
     7381 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
     7441 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
     7501 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
     7561 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
     7621 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
     7681 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
     7741 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
     7801 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
     7861 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
     7921 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
     7981 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
     8041 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
     8101 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
     8161 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
     8221 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
     8281 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
     8341 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
     8401 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
     8461 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
     8521 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
     8581 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
     8641 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
     8701 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
     8761 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
     8821 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
     8881 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
     8941 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
     9001 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
     9061 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
     9121 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
     9181 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
     9241 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
     9301 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
     9361 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
     9421 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
     9481 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
     9541 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
     9601 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
     9661 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
     9721 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
     9781 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
     9841 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
     9901 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
     9961 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    10021 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    10081 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    10141 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    10201 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    10261 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    10321 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    10381 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    10441 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    10501 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    10561 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    10621 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    10681 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    10741 tagccgcgat gaatttccgc gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    10801 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    10861 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    10921 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    10981 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    11041 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    11101 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    11161 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    11221 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    11281 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    11341 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    11401 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    11461 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    11521 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    11581 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    11641 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    11701 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    11761 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    11821 tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    11881 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    11941 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    12001 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    12061 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    12121 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    12181 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    12241 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    12301 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    12361 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    12421 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    12481 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    12541 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    12601 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    12661 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    12721 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    12781 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    12841 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    12901 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    12961 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    13021 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    13081 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    13141 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    13201 aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga
    13261 tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa
    13321 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    13381 tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc
    13441 cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt
    13501 tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac
    13561 aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct
    13621 gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat
    13681 aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta
    13741 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    13801 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    13861 tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag
    13921 tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa
    13981 tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta
    14041 atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    14101 ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga
    14161 aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga
    14221 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat
    14281 ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc
    14341 cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc
    14401 ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga
    14461 gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc
    14521 aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga
    14581 tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc
    14641 agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga
    14701 tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat
    14761 gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga
    14821 tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta
    14881 aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct
    14941 tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt
    15001 gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt
    15061 tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg
    15121 aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag
    15181 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga
    15241 cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat tttttcttgg
    15301 aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa
    15361 acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta
    15421 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    15481 acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc agtcacgcct
    15541 tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc
    15601 agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat ggaagttaga
    15661 tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc
    15721 aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt
    15781 ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta
    15841 ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga
    15901 gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg
    15961 atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt
    16021 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    16081 aataattgta
//
LOCUS       DARBER010000050        15874 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARBER010000050 DARBER010000000
VERSION     DARBER010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.050"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(310..732)
                     /locus_tag="SCK06_001529"
     CDS             complement(310..732)
                     /locus_tag="SCK06_001529"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG1151534.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(746..2152)
                     /gene="atpD"
                     /locus_tag="SCK06_001530"
     CDS             complement(746..2152)
                     /gene="atpD"
                     /locus_tag="SCK06_001530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG1151535.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2175..3077)
                     /locus_tag="SCK06_001531"
     CDS             complement(2175..3077)
                     /locus_tag="SCK06_001531"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG1151536.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3091..4647)
                     /locus_tag="SCK06_001532"
     CDS             complement(3091..4647)
                     /locus_tag="SCK06_001532"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG1151537.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4670..5212)
                     /locus_tag="SCK06_001533"
     CDS             complement(4670..5212)
                     /locus_tag="SCK06_001533"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG1151538.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5199..5723)
                     /gene="atpF"
                     /locus_tag="SCK06_001534"
     CDS             complement(5199..5723)
                     /gene="atpF"
                     /locus_tag="SCK06_001534"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG1151539.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5814..6029)
                     /gene="atpE"
                     /locus_tag="SCK06_001535"
     CDS             complement(5814..6029)
                     /gene="atpE"
                     /locus_tag="SCK06_001535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG1151540.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6082..6801)
                     /gene="atpB"
                     /locus_tag="SCK06_001536"
     CDS             complement(6082..6801)
                     /gene="atpB"
                     /locus_tag="SCK06_001536"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG1151541.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7103..9946)
                     /gene="rnr"
                     /locus_tag="SCK06_001537"
                     /pseudo
     CDS             complement(7103..9946)
                     /gene="rnr"
                     /locus_tag="SCK06_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(9949..10704)
                     /locus_tag="SCK06_001538"
     CDS             complement(9949..10704)
                     /locus_tag="SCK06_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1151542.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10807..11043)
                     /gene="secG"
                     /locus_tag="SCK06_001539"
     CDS             complement(10807..11043)
                     /gene="secG"
                     /locus_tag="SCK06_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG1151543.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11161..11439)
                     /locus_tag="SCK06_001540"
     CDS             complement(11161..11439)
                     /locus_tag="SCK06_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1151544.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11552..12520)
                     /locus_tag="SCK06_001541"
     CDS             complement(11552..12520)
                     /locus_tag="SCK06_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1151545.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12735..12779
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12803..13777
                     /locus_tag="SCK06_001542"
     CDS             12803..13777
                     /locus_tag="SCK06_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG1151546.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13782..14357
                     /locus_tag="SCK06_001543"
     CDS             13782..14357
                     /locus_tag="SCK06_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1151547.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14424..15563)
                     /locus_tag="SCK06_001544"
     CDS             complement(14424..15563)
                     /locus_tag="SCK06_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG1151548.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4335 a         3425 c         2842 g         5272 t
ORIGIN      
        1 aagtcttgtc gcagtccctt attccaaata aacggtgttc aatcatctga ccctcggtat
       61 tagctcaaaa aacggcaaaa tatgaggaac tattttccat ttgtttctct aatactcagg
      121 acaaaacgct gatttcacaa cctccgatat acggtgttcc agaagtagct tcttcgaaaa
      181 ataagctgag atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatgtctt
      241 gaagctgacc acttctgtca taacctcttt ttactgttct taaacacgga taaacatccg
      301 catctcattt tatgagtgct tagatacttt gatacgattg attgctcggt gcaacgcaac
      361 agttgcccgt ttcaattcgt tcgtatcttg ttttgctttt gcttcttcga tttggcgttc
      421 tgcgcgttgt tttgcacgtt ccgcacgaga tacatcgata tcacgttcac gttccgcact
      481 atcagcaacg attgaaacaa cattatcacg aacttccatg attccgccat taacagcgat
      541 ccaatcgacg tgggtatctg aatctgttcg ttttacacga acttcatcaa tcgtcaatgg
      601 aacgatgatt ggtgcatgtt taggcaaaat cccaagctca ccagctgtcg tacgtgcaac
      661 gacgatagct gcatggtgat catagaccaa accggcagga gttaccacat ttactgttaa
      721 atattgatcc atggggcacc cctttctagt agcctagtgt tttcgctttt tctacgacgt
      781 cttcgattcg gccaacactt cggaaagctt cttcaggaag atcatcgtat ttgccttcca
      841 agatttcgcg gaatcctttc actgtttctt ctacagggac atatgaacct ggaagaccag
      901 taaattgttc agccacgtta aagttttgtg acaagaagaa ttggactctg cgggcacgag
      961 aaactaacac tttttcttga tcggataatt catccatacc taagatagca atgatatctt
     1021 gtaattcacg atatcgttgc aatacgtgtt ggacttctgt tgctacttta taatgttctt
     1081 ctcccacgat ttcaggtgct aaagcacttg aagaagaagc caatggatct accgctggat
     1141 aaatcccttg ttctgttaat ttacgttcca agttggttgt cgcatccaaa tgggcgaatg
     1201 ctgttgccgg cgccgggtca gtgtagtcat cggctggcac atagattgct tggatcgatg
     1261 tgatcgaacc ttttttcgta gatgtgatac gttcttgcaa ttgacccatt tctgttgcca
     1321 atgttggttg ataaccaacc gcagaaggca tccgtcctaa caaggctgat acttcagatc
     1381 cagcttgagt gaaacggaaa atgttgtcga taaacaacag tacatcttga ccttctacat
     1441 cacggaaata ttccgcaatc gtcaaaccag tcaaagcaac acgcatacgt gcacctggtg
     1501 gttcgttcat ttgtccgaac accatggctg ttttttcgat aactcctgaa tctttcattt
     1561 cataatacaa gtcattacct tcacgagtac gttcacctac accggtaaat acagaaatac
     1621 caccatgttc ttgggcgata ttatggatca gttcttggat cagtacggtt ttaccaacac
     1681 cggcaccacc gaatagtccg actttcccac cttttaaata tggggcaagc aaatcgataa
     1741 ctttgatccc tgtttccaaa atttctgtac ttgtacttaa ttcgtcaaag gctggtgctt
     1801 ttttatgaat ttcgcttctt tccgcatctt ctgggaaagg tgtttctaaa tcgatcgtat
     1861 ctcctagtac gttgaatacg cgacctaatg tttctttacc tacaggaaca gagattgctt
     1921 tgcctgtatc aatgacttcc attcctcttt gtaaaccatc tgttgattcc atcgcaatgg
     1981 tccggatcac tccgtcccct aattctaacg ctgcttcaag aacaactttt gatttatttt
     2041 cgtcattttt ataaacaact aatgcgttgt tgatatctgg taaggattgg tctaaagaaa
     2101 attccacgtc gacaacggga ccgattactt gaacaatctt gcctgaactc atcttttttc
     2161 ctccaatctc aatactattc tagtgccgaa gctccggcaa caatttcggt aatttcttgg
     2221 gtaatagccc cttgtcttgc acggttgtaa gaaaccgtca gctcatcaat gatattagca
     2281 gcattatcag ttgctgtttt cattgctgtc ataccagcag catgttctgc tgttttggcg
     2341 tcaacgatcg caccgtagat caaactttca gcatactgag gcaataactg agacaagatt
     2401 tcttcttttg aaggttcaaa aatatattct tgttcgaaag tagttgcttc ttctggatct
     2461 aagtctgaga ttggcagcat tttttctaca cgaaactgac tagttagcga attgatatga
     2521 tggttataac atacgtataa ttcatcgaat acttcgtttt ggtacatagt tgttgccata
     2581 tttacgattt tgcggacttc atcaaagctt ggctgatcag atagattgcg cagctcatat
     2641 gctagattga taccgcgtgc tttaaagaag tctgcaccag ttccgccaat tgcgatcatc
     2701 acgtaatcat cgggtgattg gtggtcttct tctaaaatag acatcgtttg tttcaatatc
     2761 gagctgttat atccaccgac taaaccgcca tctgcagtga tgacgatata acccgttttc
     2821 ttaacaggac ggctgagcaa catgctgttg taattgatat cccctctagg attttctgag
     2881 gcaatatcat ttaactgagt agcagtcaaa tgcgtaacga tttctcttac ttttgttgca
     2941 taaacctgaa acttctgtga agaagcttca gattttgtca acttagaagc agacaccatc
     3001 tgcattgccc gcgtgatttg gctcgttttt ttcgttgaag cgatacgtgt cttgatttca
     3061 tttaatgaag cacccattca tctcacctca ctatgcattt tgaatggatt tcaatttatc
     3121 ttctgtagag gaattatttc cttttgtagc taagaacatg tctttatatt cctgaatcgc
     3181 actgttcaac gcttcttctt ctggaagatc ttttgttgta cggattgttt caaagatatc
     3241 tgaatgattg gtatccagat attcgaataa ttcatgttcg aagtctaaaa tactgtctac
     3301 agggatacta tctaagaatc catgtgtcaa agcatacaaa atcactactt gtttttcaac
     3361 agctagcggt gcatgcaatt tttgtttcaa gatttctact gtacggcgtc cacgatttag
     3421 ttttgcttgt gttgctgcat ccaagtcaga accgaattgt gtaaatgctt ctaattcacg
     3481 gtaacttgct aagtctagac gtaaagtccc agcaactttt ttcattgctt tgatttgcgc
     3541 agaaccccct acacgagata cagatagtcc ggcatcaacc gctggacgag tacctgcgta
     3601 gaacaagtcg ctttctaaga agatttgtcc atcagtgatc gaaataacgt ttgttgggat
     3661 ataagcagag atatctccag cttgtgtttc aacaaatggt aaggcagtca ttgaaccgcc
     3721 acctaaatca tcacttaatt ttgctgcacg ttccaataag cgtgagtgta agtagaaaac
     3781 atcccctgga taagcttcac gacctggtgg acggcgaagc agcaaggaaa gttcacgata
     3841 agcaacagct tgttttgaaa gatcatcaaa aatgatcaag acatgtttcc cgttgtacat
     3901 gaactcttca cccattgcag taccagcata tggtgcgata taaagcaacg gcgctggttg
     3961 agacgcaccg gcattcacaa cgattgtgta atccattgcg ccatattttt tcaatgtttc
     4021 aacttgtgta cgaactgtag aatctttttg tccgattgct acatagatac agatcatatc
     4081 ttgacctttt tggttgatga tcgtatcgat cgcaatggaa gttttccctg ttttacggtc
     4141 accgataact aattcacgtt ggccgcgtcc gattggtaca agagcgtcga ttgcttttag
     4201 acctgtttgc attggttcgt taacagattt acgttgcata acgcctggtg ccattgcttc
     4261 tactggacga gctttgtcag taacgatttc acctagtccg tcaattggct gacctagcgg
     4321 attaactacc cgtccaatca aggcctctcc aactggaact tccatgatct ttcctgttcg
     4381 ttttacttta tctccttcac gaatggactc gaaatcgcca aggataatga tacctacatc
     4441 atttgattct aagttttgcg ccattccgta tgagccattt gaaaattcaa gcaactcacc
     4501 actcatcgcg ttttctaaac catgtgcacg agcgatcccg tcacctacat aagttacggt
     4561 accgatttct tcgactgaaa gcacgttttg ataattttca atttgttctt taatcaaggc
     4621 actaatttct tctgctttga tggccattcg attcacctac ctctttgtac tatctattta
     4681 attgattacg catattttct aattgcgtgc gaatgcttcc gtcgattact cgatggttag
     4741 cttcaacaat cgcgccacca ataatagaag aatcaacgat ttgttttaat tcgactgatt
     4801 gatagttcat tgtttttgca acattctttt ctaattgtat tcgttgttcg tctgaaagtg
     4861 gaactgctgt tgtgacactt cccaacagca aaccttgatg ttcatcatat cgacgttcat
     4921 attcttcgat catgaacaaa agatcatcca ttcggttata cgcataaaca acttctaaaa
     4981 agtttttaac gattccatca tagccactaa caagcttgtc catgatttct cgtttttcat
     5041 gaggttctag acgaacatca cttaacatat tacctaatcc aggtacttgt tcatagattt
     5101 gacgtaagct tacgagatct tgataaattt cttctgcttt atttgaatcg atcgccagtt
     5161 caaataaagc tttcccgtaa cgtcttccta ctgtatattt atctagtttc atttgaagaa
     5221 cctagacctt cgatatattg attgattaat gattcgtgca tttctggaga taattctttg
     5281 ttcaagattt tttccgcgat ttgaagagat agttctgcaa catcgtcttt tacagagttc
     5341 agcgctgaat cacgttctac agtgatatca gcttgggctt tactttttag acgagctgct
     5401 tcttcttgag catccttcaa aatattttgg cggcttaatt ctccactttc tttcgcattt
     5461 ttgatgatat cagctgcatc agaacgagag gctaacaatt gttgttcgcg ttcttgttcc
     5521 attttcgctg agttgatgcg agattgttct gcagaatcca aatcattggc gatcttgtct
     5581 tcacgttttt tcaaaatatc gctgattggt ccccaagcaa aatgcttcaa gagagccagt
     5641 agtatcaaaa atgagccgct gacaacgatg acatttccta acatcgcatt gccaacttct
     5701 gcaattgcca attgattcag cataggcgag ccttcctttc tttgaagtac ttctagtttt
     5761 catgtaaaaa agcagtaaca aaccaaattg tttctgcctt aatactgatt caattaaaca
     5821 ccaaaaacta gaatcaaggc gacaactacc cctaagatag gaacagcttc gactagagct
     5881 accccgataa acattgttgt acggaattga ccagacattt caggttggcg agccattgat
     5941 tcgattgttt tagagataac ttgaccgtta ccataaccgg ctccgattgc tgctcccatg
     6001 attgcgattg ctgctgcgat ataattcata attgttttcc tcctaaatta gataaatagt
     6061 agtttgtttt taaaacgaat attattcttc cacttcaatc ttatggctca tataaaccat
     6121 agataaagta acgaagacaa atgcttggat gcttccgata aacaaggaga atgcgatcca
     6181 gaccatctct aatggaattg ctaacggcaa tgagaaccag cctaagctta ttaccatttt
     6241 tgcaatcaag gttagcaaaa cttcccccgc ataaatattc ccataaagac gtaaagcaag
     6301 tgttaataag tttgtaaatt cttcaatcag tttcatcgga aacataaaac tatatggttt
     6361 caagaacgaa tttacgaaat atcctttgaa gcctaacttt ttcaccccaa ataagtgagt
     6421 aagtaagatc attataaatg ccaaagttaa agtaacaaac gggtctgctg tcgggctttt
     6481 ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact aaaccgatgt tgtttgcaac
     6541 aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa ttgctcaatt cttttctagg
     6601 cagattatct gttacgattc ctcgactaaa atcaatgacc cattctatcg cattttgttt
     6661 gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa gctaagaaga aaacaatcaa
     6721 acaggtcagt aacaccatta tacaaactgt accatcaaac caaattggtc caatgtggaa
     6781 cgttagcgag cgttcatcca atccatttca cctcttttcc ccgaaattca cataaaatca
     6841 attcatgtgc gtatctggtt ttttccaatc ggtttctaac cacacggtat cataccacca
     6901 tgaaacagaa aattcaaaac attttcatga aaattagaca cttttttatt tcagtcaaaa
     6961 acgaagcgaa tttttcttta tacacggtgc tcctgatcta agaaaaaacc tatgataatc
     7021 atgttttatc agattaaatg ctaacaaatt ttatcattaa gtaaaagcaa aagcaccgaa
     7081 tatgcgaaaa aggataatga aattatctag agaaattttt taatgttcga tcaatttgtc
     7141 gatcgatatc ttttcgtttc aaatcttccc gtttatcata agattttttc cctttcgcca
     7201 gaccaatcaa taccttggca tagccgttac gaatatagac ttttaaagga acaacagtga
     7261 ttccagtatt tttcaattca ttttcaagcc ggatgatttg ttttttgtgt aataataact
     7321 ttctcgtgcg caacggatca tgattaaaaa tatttccttg ttcataagga ctgatatgaa
     7381 cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa tccatctttt agattgatcc
     7441 ggctgtttcg tatcgacttg atctcggttc cttgcaagac catccctgct tccatcgtgt
     7501 cgatgatcga ataatcatgg cgagctttct tgttttgtgc aattaatttt ccctcgcctt
     7561 ttggcatagc tgtttcctcc aactcatttt attttttctt tttcttcttt ttaacacctt
     7621 tgtaaaatgg cttcttccct tttttcttac cgaacgtttg cttactatcg ctatatcgtt
     7681 tgttcttctt gccatttctt tctgggcgtt ttcctgcttg ttttccttcg cgttttcctg
     7741 attttccatt tcgtgttttt ggccgttcca acgctgtaac ctcttctgcg gaaacaagtt
     7801 caaaatctac tgctcgttca tctggatcag ctttgactac ttttactcgc actttctgtc
     7861 cgattttaaa agtcagacca gttctttcac ctacaagagc taaatgattt tcaacaaaat
     7921 ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc ctctactgta ttcggcagtt
     7981 cgataaacag gccgaatttc acaacagagc tgatgatccc atcgaattct tcccccactt
     8041 tgtccatcat gaattctgct ttcttcatgg aatctacttc acgttctgtt tctactgcac
     8101 gacgttccat cttagaacta tgatctgcaa tttcaggaag ttccgtagcc cacgaacttt
     8161 gattgctttc acttttatct tcactgtaag aacggatcag acgatggacg attagatcgg
     8221 gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc tgccgctaag ccataatgcc
     8281 caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct tagcaacata gtattgataa
     8341 ctgcagcttc tggtttgtct tctactcctt cgatcacatg ctgtaaatct tttggtgtga
     8401 tcgttccttt cgttcctcgg accagaatgc ccaaagctga agcaaaatca aagaagcgct
     8461 gcattttttc ttcttttggc tgttcatgga ttcggtaaat aaacggcaga tttaatttat
     8521 tataatgttc agctaccgtt tcattggccg caagcatgaa agattcgatc aagcgttcac
     8581 caattcctct gcttcttagc tggatatcat ttgggtgtcc ttcagcatca acaaggattt
     8641 ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat tcgcatcgtt tcaaggattc
     8701 gatgcaattc acccatttct tggaacatag gtacgagctg agcatagcgt tgcatcgttt
     8761 cttcgtcttg atcttctaag atttgattga cagctgtgta ggtcatccgt tctgttgttt
     8821 ggatcacact tgggaaaata tcatgagaga ctacttgtcc atttggatca atctccattt
     8881 cgcaactcat ggtcagcctt ggaacatggg gattcagtga acagatacca ttcgataaac
     8941 gttgcgggat catcggaatc actcgatcag tcaaatatac acttgtccct ctttcatatg
     9001 cttctctgtc caattgactg ccttgtgtca catagtaaga aacatctgca atgtgtactc
     9061 caagaaaata attaccgtta tctaattttt taaccgtaac tgcatcatct agatcttttg
     9121 catcttctcc atcaatcgta acaatgagct gatttcttaa atctctacgt ccagttatat
     9181 ccttttcgct gatttgatct ggaacttcat ctgcttcaga caatacatca tcaggaaatt
     9241 tcgtagggat accattagcc acgacaattg agaggatatc catgccaggg tcattcttat
     9301 gaccaatcac ttgcttgatt atcccttcta aactagtcgc ataccctttt tcagggtaat
     9361 gggtcagttc gatcagcaca atacttccgt caactggacg aactccctct gctgctgcat
     9421 atactttaaa ttggttgagc tttctatctt tcgggataac aacgccgtaa agatccgttt
     9481 cttgtatttc ttcttctgaa taagcaacga attcccctgc taactgagta gtcgcccggt
     9541 ctcgaatctc gactactttc ccctctgctc cgcgatccat atctgtattt gctcgcttca
     9601 tgatatcgat tgctacggtg tctccatcca ttgcaaaacc agtcgcttct tttgggatat
     9661 agatgtcatc ttcttcggga tcaatcgtta caaatccaaa tcctcgttca tttgctcgaa
     9721 atgttccttc gatcaagatt ggttttaaag gaagtttcac ttttcctttt ttattgaaaa
     9781 ctatactctg ttctctttcc atttgagcaa tcgtttgtac tagtaattta aaatcatcgc
     9841 ttttttggaa ccctaaacct tcagcgattt cttccataga aaaactgcgt ttgcgactgc
     9901 tttccatgaa aaagagaatt ttctctttta tcgtttcttt tgtcatattt attcctcatt
     9961 ccaaggtaat ttttctaaaa attccaagac gtctttttct aattcgcgtc ttgctgtacc
    10021 aactgtaatc acatgaccac tttcaggata ccatcgaagt gtcactcgct gacggctcaa
    10081 cttgcgtgca gtttcaaata cgccattagg gtcgatcatt tcatctttcc cagcttgagc
    10141 taaaaatatc ggctgttgga tatctgacaa cctactttct acgattgctg cctgatcttg
    10201 tatatccatt aattgttgtt cagctaacga gcggtaagca actgcttttt cattgatttc
    10261 tttctctgat ttacctgctg tcttcagcac ttgacgtacg tatttttcaa agttttcagg
    10321 cacgtttgtt tttacaggag acaaaggcga acagaaaaaa ccgccgccaa tcactgattc
    10381 ttcttctagt gctcgtacag caaagatgcc gcccattgat aatcctaata cggcaatatc
    10441 agaaaactgc tcagactgta aaaaatggat ggctttttta gtgtctgccc accagctttc
    10501 tgctttttct tgcaaaatat catatggctc cattgttccg tgtcctttaa aaagcggcgc
    10561 ataaactgta taatctgctt tttccaaaaa tcgtgcgagc attcgaacat cgtttggact
    10621 tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca tgttttgcat aaaggggttt
    10681 tggtaattgt ctcactcgtc tcacctcttt cgcctcattt taccatagtt tctttcataa
    10741 cttcttctta agcgttctct ttgcaataaa aaaagccgcc cgccgttttg cgagaggctt
    10801 attcaactac ttcgaagata aatatgctag gacaaataat aagatcatcc agatagctcc
    10861 cataactgca gttgatcgct gcatgacagc ttcaaagccg cgagcttttt gtttgccgaa
    10921 tagttgatcc gcaccgccgg taaatgcact cgctgcacta ttttgcttgc ttggctgcat
    10981 cataatcgca atgaccataa caatcgataa aacgatcacg attcccaaaa ttaaattata
    11041 catatcgcag cctccttccc aaaagatatc gtctctttaa ctttatcata gataaaagtc
    11101 cgtgacaact ctatataaat ggaataacag ctgaaagttc aaaactttca gctgttattc
    11161 ttatgactga cgtaagacgt cttgatattc tggtttcttt tcaaactctt tttttgcata
    11221 agggcataaa ggaatgatct ttttcccgtc tcgtcttgct aattctactc cagtatacac
    11281 tagcttttca gctaactttt gaccacgata tgctggatct acaaatgtat ggtcaatgat
    11341 catgatgtct gggcccgcat ctgaccatgt catttctccg atctcttgtt gttcatcatt
    11401 gaataaagca agacgatttt tttcttcttt gatttccata tgccgcactc ctcaaataac
    11461 tgatattttt ttattcctac cttattttag cagtctcctg atctataaac aattatttat
    11521 cctcgaatta cttacaaaaa agaagagaaa attaattcat cgttattctt tgttcaataa
    11581 atgaaaaaat cactgtccaa taggttttcg gatctttcat cgatgcttcc aagtgttctg
    11641 cttctggaac aagcagcagt gctttatccc ctgccgtagc attttgaatc gtgtacgctg
    11701 cttctgtagg gacaaaatga tctctttcac catgaatgac caataaaggc agatggttgc
    11761 tgccaagctg tctcgtagct gatgcttgct ttaaggagta accggcgtat ttctgggcat
    11821 aatgattcgc atatctcatg accatttttt taggaaagac agtgatttta cttaacatat
    11881 atcggaattc tgcataaaca gaaacatatc cgctgtctaa aataaatcct ttgaccgctg
    11941 atggaagttt ttcaccactt gccatcatga tcgtagaagc tcccattgat ccgccgtgta
    12001 aaataatgga cgcattcggc tgttcatcta agatgagttg gatccaggca attaaatcta
    12061 gccgatctag ccatcccatt ccgatgattt ctccttcact ttctccgtgc gcacgtaaat
    12121 caggtacaag aacgttgaat cctttctctg catatctttt acctatatga gacatgtctc
    12181 ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca tttattctgc ttatttggat
    12241 ggatgaacat ctgaccagct agtttcaacc cgtcataact tttgattgtt ttgggtgttc
    12301 cctgctccca aaagctttca cactcttctt ctaatttttg tcgttttttt tctactgacg
    12361 tgactgcagc agggtccatc aaacgatgac cagtgtcttc ataccagtca tatcctctct
    12421 gtactcccgc tcttactaat ttctttgcat aaaaataaga aagaaaatgg taactgatcc
    12481 ctataattat cagaataaga atgaaataaa aaataaccat tgtacacccg ccgctttctg
    12541 ccaaactata atctaactat tatccaatgt accgatccat aggattcttt attttcggca
    12601 cacgtaaatt ccatttcttt ttcattgtat catttactta aagttctcct caatagattt
    12661 acttgtaaga gagcatttac attcgcaata aaagccagta tactaatttc gtgcattata
    12721 atcaagacgc tcgtcttgga ctggttcgga aacttcccag aataaaaaac taagtatctc
    12781 taaagaaagg agattggaaa taatgcgaaa agtaattatt gattgcgatc cagggatcga
    12841 cgacacttta gctttatcat tagctgtcaa atcccctgat atcgaagtag ttgctattac
    12901 cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag aatgtgctga aatgcctgga
    12961 acgctgtgat cgtttagaca ttcctgttta tcaaggaatg gaaaaacctt tgaagcaacc
    13021 tttcatcagt gcacaagaca ctcatggtat ggatggttta ggggaaacaa atttcccgat
    13081 gatcttgaga aaacaggcag aacctctcca tgccgttgat tttttggctg attatttcaa
    13141 agaaaagaca aatacatcag tcattgcttt gggtcctttg accaatatcg cttttgcttt
    13201 gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt gtctcgatgg gtggaactta
    13261 caaaagccat ggtaactgtt cgccagttgc tgaatacaat tactggtgtg atcctgaagc
    13321 agcttcttat gtattcgaga atctcaaaca aacgatagaa atggttgggt tagatgtgac
    13381 aagagaaatc gtcttgacac cgactatcct cgaatactgc tgtcaaatga atcctgaaga
    13441 gggagagtat ctaaaagcga tcactcgttt ttattttgac ttccactgga aacaagaaag
    13501 aattttagga tgtgtcatca atgatccgtt agctgttgct tattttatcg agggggccat
    13561 ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa ggaataagtc gtggacaaac
    13621 attggttgat cgttatgaat tttggcagaa acctgctaac agtaaaatta tgacaaaagt
    13681 agatactcgt ctttttttcc gaaagttttt aaccgttctt ttaaatgcac aagaagaaac
    13741 gattatgaaa gatttggaaa ggttaaagat gggctaacaa aatgaataaa aacaaaatgg
    13801 caactcgcgt acttacagtg atggctttgt gtattgggat caattatatt ggaggaacga
    13861 ttgctttatg gcttcgtttg ccaatctatc tcgattctat cggaacgatt tttgctggtg
    13921 cattgcttgg gcctgtacct ggaatgctga caggattgtc cagcggtatt ttaagtggag
    13981 tgactacaga tattttttct ctgtattatt cgccaatcca aattctcaca ggcttgcttg
    14041 ctggattgat tttgccaaga tatctcggca aagataaaaa cagcttgaaa ctgttattac
    14101 tcgctttgat tttgtcggta cctggaacga tcgtttcttc gatcattacc gttcagttat
    14161 ttggcgggat cacctcctca ggatccagta tgatcgttca gctattacat gggttaggtt
    14221 taagtcagac aacaagtgtg atcttggtac aggcaggtac agactacctt gatcgtttgt
    14281 tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc acaacgcttg atatattcaa
    14341 aaagaaaaat aggctgaata aacagagctg gattagcaaa ataatgctaa tctggctctg
    14401 ttttttcatt tattaaaaaa atcttacgct aagtaggata aaataattcc ttgatatttt
    14461 tcaatcatat ccaaataatt atcgatttca acatactcat ctacttgatg aggcaaagta
    14521 acgacaccag gcccaaagac gacaaagtcg aaggaatggt tagctttcgt aaattcggct
    14581 gcatccgttg ttgctgccgc tccaactaaa ggtaatggct ggctgaattg ttgctgaatg
    14641 caatgaatca aaggagaatc tggatcagct ttgacaggta ttttgttgta atcgatcatc
    14701 aattccaaat gatagtctgt ctcttgattc aattcattga cgattgattg cagcaatgca
    14761 atgattttat catttgggta ttctggaatc gatcgaatgt tcccttggag ctgcgcatgg
    14821 cttgggatgc tgttgacttg attcccgcca ctgatcaatg taacattatg gattgttttt
    14881 cctaggacag gattctcgat tgtttcagcg aggtgattca tttcagcatt tgctttcgta
    14941 atgaattcat ttaggtgatt gattgcatta taaccttggt ctggcataga gctatgcgct
    15001 tcttttccgt gcgaggtgac tgtatagttg attgatccca tgtgcgtata catcaaactg
    15061 taattagttg gctctccgat gatcaaggca tctaagtcat ctacatagcc tgctttcgtg
    15121 agctgttccc cacctagttc acccacttct tctccgacag tggctaataa tttgactgtt
    15181 ccgttaaatg gttttcctga ctctttcagt tcgatcatgg caatcaccat tgctgccaag
    15241 ccactcttca tatctgtagc accacgtccg tacaaacggt tcccatggat ttctgctgca
    15301 aagggtgcat aggtccaaga cgattcatca ccagccgcca cgacatccat atgcccagat
    15361 aggcctaaca cttttcctga ttggcccttt tggtaacgag caatcaaatt atctctacca
    15421 tctctgtaag aaacaatctc tcctgttatg tcatgctttg ccaaaagctt attcaaatag
    15481 gctgctactt caccttcgtt tccattcact gatttaattc ggatgatctc ttgtaaaatc
    15541 gcgatttttt cttctttttt catctaatcc tctcccttgc catttctttt tattgtattt
    15601 gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa aatgaagaaa gagagccact
    15661 ttatgaaaaa tatgctgcta cttccctcct attttattta tacgagagga cttgctcata
    15721 gattcctatt ctactgttct atccttaaaa gctcttatat gatgctagtt attgttagct
    15781 ttcctttttc aaggatgatc ttttcttgta ccattctttg taaacatcat atacagaagc
    15841 tagttgcaaa aaaaactctc tacctgctga tatt
//
LOCUS       DARBER010000051        15510 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARBER010000051 DARBER010000000
VERSION     DARBER010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.051"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..1475
                     /locus_tag="SCK06_001545"
     CDS             138..1475
                     /locus_tag="SCK06_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG1151549.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="SCK06_001546"
     CDS             1523..3652
                     /locus_tag="SCK06_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG1151550.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="SCK06_001547"
     CDS             3627..4316
                     /locus_tag="SCK06_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151551.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="SCK06_001548"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="SCK06_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="SCK06_001549"
     CDS             complement(5546..5740)
                     /locus_tag="SCK06_001549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151552.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="SCK06_001550"
     CDS             complement(5790..6230)
                     /locus_tag="SCK06_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG1151553.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="SCK06_001551"
     CDS             complement(6234..7079)
                     /locus_tag="SCK06_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG1151554.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="SCK06_001552"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="SCK06_001552"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG1151555.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="SCK06_001553"
     CDS             complement(8703..9650)
                     /locus_tag="SCK06_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1151556.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="SCK06_001554"
     CDS             complement(9873..10223)
                     /locus_tag="SCK06_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1151557.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="SCK06_001555"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="SCK06_001555"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG1151558.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="SCK06_001556"
     CDS             11646..11966
                     /locus_tag="SCK06_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1151559.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="SCK06_001557"
     CDS             11988..12452
                     /locus_tag="SCK06_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1151560.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="SCK06_001558"
     CDS             12657..13262
                     /locus_tag="SCK06_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG1151561.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="SCK06_001559"
     CDS             complement(13301..14590)
                     /locus_tag="SCK06_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG1151562.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="SCK06_001560"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="SCK06_001560"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG1151563.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4840 a         2899 c         3180 g         4591 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa
//
LOCUS       DARBER010000052        14877 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARBER010000052 DARBER010000000
VERSION     DARBER010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.052"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(163..1242)
                     /locus_tag="SCK06_001561"
     CDS             complement(163..1242)
                     /locus_tag="SCK06_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1151564.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1524..2264)
                     /locus_tag="SCK06_001562"
     CDS             complement(1524..2264)
                     /locus_tag="SCK06_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1151565.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(2268..2591)
                     /gene="rsfS"
                     /locus_tag="SCK06_001563"
     CDS             complement(2268..2591)
                     /gene="rsfS"
                     /locus_tag="SCK06_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG1151566.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(2648..3241)
                     /gene="yqeK"
                     /locus_tag="SCK06_001564"
     CDS             complement(2648..3241)
                     /gene="yqeK"
                     /locus_tag="SCK06_001564"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG1151567.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3225..3875)
                     /locus_tag="SCK06_001565"
     CDS             complement(3225..3875)
                     /locus_tag="SCK06_001565"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG1151568.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(3885..4196)
                     /gene="yhbY"
                     /locus_tag="SCK06_001566"
     CDS             complement(3885..4196)
                     /gene="yhbY"
                     /locus_tag="SCK06_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG1151569.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4212..5321)
                     /gene="yqeH"
                     /locus_tag="SCK06_001567"
     CDS             complement(4212..5321)
                     /gene="yqeH"
                     /locus_tag="SCK06_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG1151570.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(5318..5845)
                     /locus_tag="SCK06_001568"
     CDS             complement(5318..5845)
                     /locus_tag="SCK06_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG1151571.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6025..6810)
                     /locus_tag="SCK06_001569"
     CDS             complement(6025..6810)
                     /locus_tag="SCK06_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG1151572.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(6803..7672)
                     /locus_tag="SCK06_001570"
     CDS             complement(6803..7672)
                     /locus_tag="SCK06_001570"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG1151573.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(7673..9052)
                     /locus_tag="SCK06_001571"
     CDS             complement(7673..9052)
                     /locus_tag="SCK06_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG1151574.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9060..9479)
                     /gene="fabZ"
                     /locus_tag="SCK06_001572"
     CDS             complement(9060..9479)
                     /gene="fabZ"
                     /locus_tag="SCK06_001572"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1151575.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(9504..9980)
                     /gene="accB"
                     /locus_tag="SCK06_001573"
     CDS             complement(9504..9980)
                     /gene="accB"
                     /locus_tag="SCK06_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG1151576.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(9983..11218)
                     /gene="fabF"
                     /locus_tag="SCK06_001574"
     CDS             complement(9983..11218)
                     /gene="fabF"
                     /locus_tag="SCK06_001574"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG1151577.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(11237..11974)
                     /gene="fabG"
                     /locus_tag="SCK06_001575"
     CDS             complement(11237..11974)
                     /gene="fabG"
                     /locus_tag="SCK06_001575"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG1151578.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(11986..12903)
                     /gene="fabD"
                     /locus_tag="SCK06_001576"
     CDS             complement(11986..12903)
                     /gene="fabD"
                     /locus_tag="SCK06_001576"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1151579.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13010..13234)
                     /locus_tag="SCK06_001577"
     CDS             complement(13010..13234)
                     /locus_tag="SCK06_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1151580.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(13287..14249)
                     /locus_tag="SCK06_001578"
     CDS             complement(13287..14249)
                     /locus_tag="SCK06_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG1151581.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(14259..14699)
                     /locus_tag="SCK06_001579"
     CDS             complement(14259..14699)
                     /locus_tag="SCK06_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1151582.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         3819 a         3456 c         2676 g         4926 t
ORIGIN      
        1 catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg agtgggccct
       61 tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag ctttccagac
      121 acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg tcaaccgttc
      181 ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa tctttcgcgg
      241 gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt tgccgattgg
      301 gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg tcccgcgctc
      361 ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg catcgtgcag
      421 tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc cgaagtacgc
      481 accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat ccttcccggc
      541 cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca gcacggcatc
      601 cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg ttttccgggt
      661 accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat tcgggcggtt
      721 gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca tattcttcgt
      781 tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga taatggccgg
      841 gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc atttttccaa
      901 cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata agggccgtct
      961 gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt cgtagacgtc
     1021 atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag taacggtccc
     1081 gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg caacctgacg
     1141 gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt aggttaggtg
     1201 ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc atccttgtct
     1261 attgagagtt gtggtaactt caatataaca tggaattcac gtggcgtttt tttatttcca
     1321 aatggctaag ttaattataa aatccgccac aagtaaaaaa aggcaaaaat cggaaaaaat
     1381 acccgaacta tataaactat tctcgcaaac acaatacttc tgcgacgaaa agctgcactg
     1441 caagagcaat gcctatatcg ttcggatata taatttatgt tgaagattta ttcttttctt
     1501 gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca gcgctttgat
     1561 ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga aaagccgctg
     1621 ttttccagca ttcttaaata actttccaat ggataggtac gctcttgatg tagttcatct
     1681 tcacgaataa atcgaccaga cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg
     1741 atgctgtgtt tctcgcttcc tggataacta tcccacaaaa aagcaaaatt atccccttga
     1801 taatgatagc tatattctgg aaaaacctca tctacttgat aggtggagtg tacgtcaaat
     1861 aaaaatcggc cattttcttc taacgcttga tagacttcat caaacacttg ctgtacctct
     1921 tgtctatttt tcatatagca cagcgaatca gaaaaacagg taattgcctg atattgtccg
     1981 atttctgata aatcgagcat attgccttcc acaaattgga tcgatgcgtc ttcttcatat
     2041 gctcgctggc tggcaatcat caacatctct tctgaaaggt ctaaccctgt cactttatat
     2101 ccatcttttg caaaggcaac cgctaatgct ccagtaccgc atgccagttc caaaatattt
     2161 tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc attgctgata aagtgtttcg
     2221 tccatcactt catcgtaaat gaaagcaaat gtttcataag ccatatgtta atccaaccat
     2281 tcactaagat cgactagcgg agcatctgac catagttttt ccagattata gaaatttcgt
     2341 tctggcgcat ggaaaacatg tacgatcaca tctccaagat cgatcaatac ccattttccg
     2401 ccttcttttc cttcaatacg ttttacttca tatttgtttt cttcttcttt atcaatgatt
     2461 tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg aacagatcat gaaataatct
     2521 gccaacaagg aaacatttcg aacgtctaaa gcgataatgt cttctgcccg ttttgaatct
     2581 gctgcttcaa tcgcaatttt taattgttct ttactatcta tgatgatttc ctcctaatat
     2641 gagcagctta tttttttgct acccagtaat tatatgtttc gattgttttt ggataaatct
     2701 gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct acagcttcgt
     2761 ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct cgacctggtt
     2821 caatatagtc tgctacataa atgattttgt caaggagact catcttagca gccccagtcg
     2881 tatgcacccg gattgcatga agaatctctg catcggtgat tcctaattcc cgctctacaa
     2941 acgaagcgcc aacaagacca tgccagattg cattattata attcaataac tccggatcaa
     3001 aaccgtctct tacgattacc attttgaact cctcatctgg tcgttctttt gcataatcat
     3061 gtgtcaatgc agcaatacta gctttttcag gagaagcacc gtattttttg gcaagagcca
     3121 cggctgtttc ttctactcca agcacatgct tgaagcgttg ttcgctcatt ctcatctgta
     3181 ttttttgcat caggttttct ctagaataag cagtatattc attactatag tccatattca
     3241 tacaaccctt cattttgaat ataatcaatt actttatctg gaacaaggta gcggatagaa
     3301 cagccttctt tgatcttttg acgtattttc gtggaactga tgtcgatctc aggtacatct
     3361 acccaaatta caggatatgg agtatccgtt gtgtatcctg gtcgacgtat acccacaaag
     3421 ttgaccatag aagtcagctc atcgatttta taccattttg gcaaatattc taccatatcc
     3481 ccaccgataa taaagtaata atctgtatcc ggattgtttt gcgtcaattc cttcatagta
     3541 tcataggtgt aacttttccc accacgagct aattcaatcg tttcaatctg caaaaaagga
     3601 ttgtcttcca ccgctaattc caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc
     3661 tcatcaacat gaggtggctg ataagaaggc atcaaaaata cgcggtcaag acctaaattc
     3721 cggccagctt gttcagccat cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc
     3781 aagatcccta cttgcttttt cttctctaag aattgaggca tttcttcagg aaataattgt
     3841 gaacgaacaa atgtcttagc ttgcgtattc atcatggtca ctccttatat tgcttttact
     3901 tcttttgata gcctttgata cttttctttt gtggaaggtt tgtataatac aataacacgt
     3961 ccaatgattt ggacgatttc gcaatctatt tctttttcta atacttgagc tgcttcttct
     4021 gcaatctcat ctgtattttg aagtaacgat actttgatca gttctctttt ttctaaagct
     4081 tcttcaattt gaacgatcat tgctgaattg atcccacctt ttccgatttg aaaaatcggc
     4141 tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt
     4201 ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt tggcgcccag
     4261 cccgcaatta ctactggcgc tgttacagtg atccagccta gaccggcaaa aacgatgtct
     4321 gttttttctt tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc gacttcatca
     4381 ggacgaggag gctgtaataa tccacctaca tgtttttgat aaaattcgtc tgctttttcc
     4441 agtttcgttc gatgaatatt tacatcattt gacacataag caataaatga agcacgattg
     4501 ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc
     4561 tgataaactt taggtttgat ttcttttaac ggtgcaatga tccgcaaatc ttttttacct
     4621 aagtagtgcg ccatttgatg gcgatggata attcccggtg tatcaatcaa aaaatgcccg
     4681 tcatctaaag gaatctcgat tttatccaag gtcgttcctg gaaactgcga agttgtgatt
     4741 acatcttgta ctcccgctgt atttttgatg atttgattga taagcgtcga ttttccgaca
     4801 tttgttactc cgacgatata gacatcccgt ccatcgcggt attcttcgat tttttctaat
     4861 agttccgtca tctcatgaga tttcttagca ctagttaata atacatctac tggacgaatt
     4921 cctgcttcgt aagcacgttc cttcatccat tgtgttaatt tagttttttt caatgattta
     4981 ggtaatatat ccactttgtt cccaactaaa agtactggat tgtcaccgac aaaacggtgt
     5041 agtcctggga ttagtgaacc attgaagtca aaaatatcca ccacattgac gatcaatgca
     5101 tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct
     5161 tgggtctcat tataatggcg taatctgaaa caacgctgac aatagacttc cccggaagct
     5221 aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt
     5281 ataacggcac cacagccgat acattgtaat tcttctgtca ttcgattcca cctcgccagc
     5341 ccatatcagg gtgttttgct aatagatagg ccataattct tttttcccaa aaacgattaa
     5401 attgtgtctt ccaactatct gttgcgataa tgggttttac aaggatgcta cgtatccctg
     5461 cacgattggc tccacgtata tcagtcataa tctgatcgcc taccattact atctcatcat
     5521 ctgctaaatc caattgtttc tttgctatgt tgatccctct tgctaaaggt ttcattgctc
     5581 gtgcaacata caaaagatca aatttttcaa tcgctcgcgc gacacgtttt gaattattgt
     5641 ttgaaacaac aacaacaggg attcctgcat ttttcatttc taaaatccaa gtaagcaact
     5701 cttctgttcc atctggatta ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg
     5761 ctttgatgcc tagattcttt aattgggcag gtgtaatctg atagatggct tctaccatcc
     5821 atgttggttt gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca
     5881 taaagtactc ctcaaccccg tattatactg catatcagca aaaaaaacta cctctatact
     5941 cgttcgtctt tcagaaaaag catttcttta caaaatgacc aaaaataaag attgatcaca
     6001 gaaaaattta gaaacaaaaa acaactaata ttggcggaac ctcttgtatc gtttttccag
     6061 taatttatgc tgatctaatc gaacaagttc cgtcaatctt tcaatcaacg cttttttcat
     6121 aatgtgtgcg actttatatc gtggcaactg ctcgccgttg aacgtttcag gaataactcg
     6181 atcaacgatt cctaactcgc gtagttctgg cgcagtaatt ttcatcaact cagctgcttc
     6241 tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat ccttctggag aaagcacagc
     6301 ataaatcgtt tcttccagca tccagacttc atccgctaaa cctagagcaa gggcaccgcc
     6361 acttccacct tcaccaatga tgatggcgat gatcggtacc tttaaatcag acatttcgag
     6421 cagatttctg gcaatagcct ctccttcgcc gcgctcttct gctcctacac cgcaatatgc
     6481 acctgctgta ttgatcaagg taatcaccgg tctattgaac ttttccgctt gtttcatcag
     6541 tcgtaaagct tttcgatagc cttcaggatg gggagaacca aaatttctgc ggatattttc
     6601 ctgcagatcc cgtccttttt gaataccgat cactgttacc ggtttgccat ctaatcgagc
     6661 gattccacca acaatcgctt cgtcatctgc aaataaacga tcaccatggc attcctcgaa
     6721 agaatcgaag atctcttcaa aaaaatccaa gcttgtccat cgttctttag ctcttgctaa
     6781 ctgaacgaca tcatgtgctg tcttatccac gatctatcca tcctttctgt gtatgcaatt
     6841 caagaagcgt atggattttc tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac
     6901 catgagataa taagaattca gccttttgga aatcttctgg aagttcctgc ttgatcgtct
     6961 gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg gggttctgct agaataatat
     7021 cgccttccat tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt aaaacagtta
     7081 gataaaaaag accttcattg ctatgccgtt tgacagcagc agagattttt gccatctgca
     7141 ttagagaaaa gattccttct tgcatccgtg cgccacctga agctgtgaac aagacgactg
     7201 gaagactttc ctttgttgct cgttcgaata atctggtgat cttttctccg acaatcgttc
     7261 ccatacttcc catgataaaa ttaggatcca tcactccaat aatgaaggga atcccctgaa
     7321 tggttgcttt accagtgagg actgcttcat gaagtcctgt tttatcttgc atttttttaa
     7381 ttttttccgt atattcggga aatgctagcg gatctttggt tacaagatcc gtatcccatt
     7441 cttcaaaact tttttcatca ataatcaaag ctagacgctg ccatgcaccg attcgaaaag
     7501 cgtatccgca atgagggcat actttttctt caccgatgtc tttcgtataa aggatatgtt
     7561 tgcaatttgg acactttgcc cacatgttat caggaacaga tggcgagtta gcaggtttgc
     7621 tctctctatt aggattgatc cgaatatatt tctttttctt aaacagagcc atctatgcgc
     7681 ctcctatctc cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc
     7741 tgtaatcacc tgctatgact gcaggatgac taatcaaatc catctgaaat tctgcgttcg
     7801 tgatgattcc ttctgtgata aattcactta aggctcgctg catcttcatt aatgcttcaa
     7861 aacgcgtatt tccatggacg attatttttg caatcattga atcataatat ggcgggatcg
     7921 tatatcccgg gtaaactgca ctgtctactc gcagtcccat cccacctgct ggaagcagta
     7981 agttttggat cttccctgga gaaggagcaa aatggaaagc aggattttct gcattaatcc
     8041 ggcattcgat tgcgtgtcct tggaatacta cttcttcttg acacacattc aaaggctcac
     8101 cggctgctat ctccacctgt ttttttacta aatcgattcc tgtgaccatt tctgttactg
     8161 gatgttccac ttggattcga gtattcattt ccatgaaata aaaatcgcct tcttcatcca
     8221 tcagaaactc gatcgttccg gcattttcat aattgactgc tttagctgct cgaacagcag
     8281 cgtctcctag tgcttctctt tttgtttgtg agatcactac agaaggagat tcttccagta
     8341 ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa
     8401 atcgatcacc taagatctga acttctatat ggcgagcagg ataaatgatt ttttccaaat
     8461 acatatcatc atttccaaat gccgctgctg cttcttgctg agctgaagaa aaatgctgtg
     8521 ggagctcttc ttttgattga actttccgaa tgcctttccc gccaccacct gcagctgctt
     8581 tcagcatcac tggataacca attctgtctg ctatttccaa tgcttcttct actgtagata
     8641 atacaccagt acttcctgga atgactggga catttgcttc ttgcatcaat tgacgcgcat
     8701 tgatcttatt tcccattgca tcgatcgttg cacttttagg accaataaat gtgatattgc
     8761 actcttcaca catggaggca aatcggctat tttcagataa aaaaccaaat cctggatgaa
     8821 ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc ctgaacattc agatatgaat
     8881 caacggcttt tgccgggcca atgcagattg cttcatctgc taactgtgta tgaagtgctt
     8941 cggcatctgc ttctgaatat atcgctacgg tttggatccc tagttcgcgg catgcacgaa
     9001 tgatacgtac agcgatttca ccgcggttag ctatcaatac tttagaaaac atatttcacc
     9061 tatccaatca taaaggttaa ttccgcttcg gctacttttt taccgtctac tttagcgatt
     9121 cctttgccta ttccggctga agctttcact ttcaatatct caacttcaag gtataaggta
     9181 tctcccggtg taactttctt acggaatttt gctttgtcca atccgccgaa ataagccgtc
     9241 tttcccttga attgttcaag agaaagtaat gcaactgctc ctgcttgagc cattgcttca
     9301 acaatcaata cccctggcat aacaggttct tctggaaaat gtccttgaaa aaagggctca
     9361 ttgatcgtta catttttctt agcaacgatc cgttctcctt caaccatttc ttccactcga
     9421 tcaatcaaaa gcattggata acgatgtgga atgatttctt ttatttcttg tatgttcatt
     9481 gttttccctc tttcctgctt tgcttatttt tgctgaatcc taaagagtgg ctgtccatat
     9541 tcgacaactg actcgttttc tacaaggatt tctgtgatca ctccgtcggc ctcactagtt
     9601 atctcattca tcagtttcat cgcttcaata atgcagataa cttctccttt ggcaacttga
     9661 tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt gcagataaac gattcccacg
     9721 attggcgaaa caacctcgat tccatcaaat actgccgatt ctttttcttt cattatttct
     9781 ggagcagttg ttgcttgttc aggtatgaca ttgactggct ccgaaactgg caacgtttcg
     9841 tttgtttgaa ccatattcat gcctcgactt gttttatttt tattcatata caattcaaat
     9901 tgaccttcac gcaaatcgaa ttccgttagc gaggattgat caaattgact gactaattct
     9961 ctgatttcat tgatatccaa aattattcct cccagcgttt catgcacaga accgcattat
    10021 gtccgccaaa ccctaatgaa ttactcattg cataagtaaa acttgcaggt ttgctttcgt
    10081 ttttgacaac tgtcaagtca atctcaggat cttgtttttc tacattgatc gtaggtggga
    10141 taaattgatg ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg gctgctccca
    10201 ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta
    10261 atgcgatttg gattgctttt gcttctgcct ggtcattggc tggtgtactt gttccgtgag
    10321 cattgatata tccgacttgt tccggctgta caccagcttc ttccatcgca agcttcattg
    10381 ctttagatgc accagaacca tctggagtag gagctgtcat atggtaagcg tcacagtttg
    10441 caccgtatcc aacgatttct ccatagattt tggcaccgcg agcttgtgca tgttctaaag
    10501 attcaagaac tagaacgcct gctccttcac ccatcacaaa accattccga ttttcgtcaa
    10561 acggaatcga accacgatta ggatcagttg attctgagag tgctgttaaa gctgcaaatc
    10621 cagcaatccc catctcacaa atcgttgctt ctgttcctcc agcaagaatg acatcagaat
    10681 agccatgttt gatgttacgg aatgcttccc caattgaatt cgttccgcta gcacaagctg
    10741 ttacgatagc tgtacaaata ccttttgcac ctacacgcaa agcaatattt ccagctgcca
    10801 tgttggcaat cgccattggt acgaacattg gagaaactcg ttttgcacct ttttcatgta
    10861 gacgaataac ttggttttcg attgttggaa gaccaccgat cccagatccg atgatgacgc
    10921 caaaccggtc ttgattcatt ttttctttat ctaaaccgct catctccatt gcttctaaag
    10981 cagcgtagat tccatagatc gaaaacatat ccattcgttt gctatctttt ttaataaaat
    11041 atttatcgta agggaaatct ttgacttcac ccgctaaggt aatccctgtt tctgaagcat
    11101 caaatttagt gatcggtgcg atgccattcg tcccctcttt taagctattc aaaaagctgt
    11161 ctgcgtcatt tccaataggt gaagtgactc catagccagt aattaccact cgattcattt
    11221 gtttgctctc cttttcttat ccgtgcatga ccaatccgcc atcgacattg atgacttgtc
    11281 cggtgatata tggattttgt gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc
    11341 cgaaacgctg catcggaatt tgcttttccg cttgttcctt gactttgtct gctaaaactt
    11401 ccgtcatatc agttgtgatg aatcctggcg caatggcatt acatgtaatg cctctagttg
    11461 cagcttctcg tgccacagat tttgttaatc ctactacgcc agctttacta gctgcataat
    11521 ttgcttgacc aatatttcca atcaacccag atacactaga gagattaatg atggcccctt
    11581 ctcgctgctt catcattttt ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat
    11641 tgatatccag acattttttg aaatcttctg catccattcg catcacaagt ttgtcatttg
    11701 tgattccggc attgtttact aacacatgga tcgatccaag cttttcttct gcttctttga
    11761 tcatttgtcc cgctttctca taatcagaga tgtcaccgga aactcctacg cattttacac
    11821 cgaatgcttc gatttcttct atcttttctt ttggaatctc tccgcgacca ttcaaaatga
    11881 tatttgctcc agcttttgca aaagctaagg ccatcgcttt gccgattcct cttgtactac
    11941 ctgtgataaa tacatttttt ccttttacat ccataacgtt cctccttata gtattgctat
    12001 tgtttcatca aatgtctgct tattctcaac acgcagaact ggtattgttt tatcgatttt
    12061 tttcataaat ccgcttaata cttttccggg gccgacttcg atgacttgtt cgatgccgat
    12121 tgtttttaac ttatggatac tctcatagaa acgaacaggt tgcatgactt gctgttcaag
    12181 tagtcccgcg attgtttctt tcttcattat ttccgttgtt gtgttgctta tgataggaaa
    12241 agaaggttca gagaattgaa tctgacttag gtccttcgcc agtttttttg ctgccggttc
    12301 taaaatagct gtatgaaaag gcccgctgac atttagaggg atcattcttt tgaacccttt
    12361 ttcttttaat agtaaaacag cttcgtctac tgctttttct tcgccaccaa tcacgatctg
    12421 ctgaggtgta ttataatttg caggagatac gatgccgtac ttgctagctt catgacagct
    12481 ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc attttcccgc ttccagctgg
    12541 ggcagcttct gtcatataag cgcctctttt tgctactaac gcaactgctt cagtgaagga
    12601 tagcgcatta ctagctacta atgccgaata ttctcctaaa ctcagtccta aagctgcatc
    12661 tgggatgatt ccatgctcct tcaataagcg ataatacgcg atgctgactg ttagaatagc
    12721 tggctgtgta tattgcgtct gatccaaacg ttcattttct gtaaagcaaa gttcagccat
    12781 atcgtagccg agaatctcac ttgcttcatc aaatgtctgc tttacaacgg cttcttcata
    12841 taaatctttt cccatacctt gatattgtgc accttggcca ctaaataaga atgctgtttt
    12901 catgtttttc tccaatatat ccagaatata tgtttgattc ttcctattca aaaaggccga
    12961 gacattccta tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat tatgcagttt
    13021 gtttttctac gtaagtcact aaatcttgaa ccgttttgat tccgtcttct gtttcgattt
    13081 tcacgtcaaa ttcatcttca atttcattga tgatttggaa taaatccaag ctgtcagctt
    13141 ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc agtttctttt cctaattctt
    13201 ctgcgatgat ttcttgaatt ttttcgaata ccataataat atattcctcc gatattgttt
    13261 gctttttatt tttataattt aacagtctac aactccatga gaatacttcc ccatgttaaa
    13321 cctccgccat agcctgtaaa tattactttc tgctgttttc ctaaggtgat ctttccacta
    13381 gaaactgctt catccaataa gatagggatg cttgcagcag atgtattccc atatttatcc
    13441 atattggtca ggaacttttc ttgcggcatc tttacttttc gggcaatttt ctcaataatt
    13501 ctgaggtttg cctgatgcaa aagcagataa tcaactggtt tgtctttcat gacttcttgg
    13561 atattgtttg caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa
    13621 taatacgaac cttctgaatc ttctgaatag aacggatttt tattttgctg gtagccagcg
    13681 gtcaatgagc tacttctggt accgtcagct gctaattttt ctttcagcaa ttttttctgt
    13741 ggattcgctt ctaagagaat cccacctgct ccatcgccaa acaacacagc agtcgtacga
    13801 tcagaccaat cgattagctt agatagtgtt tcaccaccaa tcaccagtcc cttagttgag
    13861 ccgcagcgga tcattttttc agccatgctt aacgcataaa caaatcctga gcacgcagca
    13921 ctgatatcaa aagcgatagc atgagttgct cccaatttcc cttgtacttg tgctgctaca
    13981 gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa taaaatccaa atctttaggg
    14041 tttagatttt tctgttttat caatttttcc gccactaaaa tgcataaatc agaagtctcc
    14101 tgatttgtta cacatcttcg ttcacgaata cccgtccttg acgaaatcca ttcatcagag
    14161 gtatccatca tcttggaaag atcattatta gtaataatct tttcaggaag gacgctggct
    14221 gttgcagtaa tcattccaaa ttgttccatt aaatatcctc acttatagtc ttgcaagaag
    14281 tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt cctccgtagc cattccgtct
    14341 aagatgttct tgaccatttc acgatgaaaa tgctgatgga cccgaaatag taactttcct
    14401 tttttcgtta gtcccaactt tacaacgcgg cggtcatctt cactgcggat acgttcgaca
    14461 tagccttttt ttactaactt gttaatagca gtggtcaagg tcccaacagt aattgatagt
    14521 tctttggcta cttctgaagt tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc
    14581 atctctgtga ttgacagatc tttgaattga gattttttca gctcagactc ttcgattgtc
    14641 aaaatatcat tgaagacact aacaagataa tcatttactg tttttaaatc tggttccatg
    14701 ttccaccgtc cactaattag tttgattatc aaatcttttg atgttcaaac tatatcttaa
    14761 cttttttcaa aatgcaacct taaaagatca tttttttact atatgagctt tttttaagat
    14821 tttactcatt taaaacaact gataaatcag catttataat ttcttttatt agaaaaa
//
LOCUS       DARBER010000053        14815 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARBER010000053 DARBER010000000
VERSION     DARBER010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.053"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(296..1024)
                     /locus_tag="SCK06_001580"
     CDS             complement(296..1024)
                     /locus_tag="SCK06_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG1151583.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="SCK06_001581"
     CDS             complement(1002..2576)
                     /locus_tag="SCK06_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1151584.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="SCK06_001582"
     CDS             2805..3743
                     /locus_tag="SCK06_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1151585.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="SCK06_001583"
     CDS             3755..4666
                     /locus_tag="SCK06_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1151586.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="SCK06_001584"
     CDS             4690..6162
                     /locus_tag="SCK06_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1151587.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="SCK06_001585"
     CDS             6213..6824
                     /locus_tag="SCK06_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1151588.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="SCK06_001586"
     CDS             6854..8767
                     /locus_tag="SCK06_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG1151589.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="SCK06_001587"
     CDS             8895..11876
                     /locus_tag="SCK06_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG1151590.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="SCK06_001588"
     CDS             11873..13534
                     /locus_tag="SCK06_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG1151591.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="SCK06_001589"
     CDS             13574..14506
                     /locus_tag="SCK06_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG1151592.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DARBER010000054        14599 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DARBER010000054 DARBER010000000
VERSION     DARBER010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.055"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(156..722)
                     /locus_tag="SCK06_001590"
     CDS             complement(156..722)
                     /locus_tag="SCK06_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1151593.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(722..1678)
                     /locus_tag="SCK06_001591"
     CDS             complement(722..1678)
                     /locus_tag="SCK06_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG1151594.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1678..2334)
                     /locus_tag="SCK06_001592"
     CDS             complement(1678..2334)
                     /locus_tag="SCK06_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG1151595.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2704..4722
                     /locus_tag="SCK06_001593"
     CDS             2704..4722
                     /locus_tag="SCK06_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1151596.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4792..4998
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5149..7563
                     /locus_tag="SCK06_001594"
     CDS             5149..7563
                     /locus_tag="SCK06_001594"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG1151597.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7911..9464
                     /locus_tag="SCK06_001595"
     CDS             7911..9464
                     /locus_tag="SCK06_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1151598.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9845..11629)
                     /locus_tag="SCK06_001596"
     CDS             complement(9845..11629)
                     /locus_tag="SCK06_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151599.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11644..13365)
                     /locus_tag="SCK06_001597"
     CDS             complement(11644..13365)
                     /locus_tag="SCK06_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151600.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13465..14226
                     /locus_tag="SCK06_001598"
     CDS             13465..14226
                     /locus_tag="SCK06_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1151601.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4738 a         2697 c         2908 g         4256 t
ORIGIN      
        1 aaaaacagct tctcatgttt ttgatttggt tggacttatt accgcagata taagcttttg
       61 agcaccgtga ataaagaggt tatgaaatca acgctctaac ctgagtatta gagaaaaaaa
      121 cgcaaaacat atcatttatg ttttgcgttt tttatttatc tttttttctc aatacaatat
      181 aaaataggcg gctggttctt ttggttgata aaccgataat tcagcacatt atatgtttcc
      241 tgtggcagtt tggaacaaaa agaatcaacc atatccagtt ctttttctcc accttcatga
      301 ccatagtaga cgacaaggat caatcgtccg cgaggcacaa gtcgtttcaa tatttcttcc
      361 atagctgtgc gtgtagtctc tggcatagtg atgattgctt tatcactttt tgggagatac
      421 cctagattga aaatccctgc tttgagattt tgttgttcag ctatcacttc tcctaacgtt
      481 tcgtggcctt gtagaaacaa tgagacacgt tcttctaact gacgctgttc tagcctttca
      541 cgagtagcat cgattgcttg ttgctggata tcaaaactat agacatgacc agtttttccg
      601 atgtgttctg ctagaaaaac cgtatcgtgg ccattgccca tcgttgcatc tacgacgtga
      661 tcccctggtt gcaagatttc ttgcagtaat gtatggctga aatgcaaagc agtttgcaac
      721 atcagacgct cacctttttc cgtacatcat attttccttg gaagctatct ctgcgaagaa
      781 gttcttcatc aattgcattc aagacttccc attttttcaa gctccacatt ggtccaatca
      841 aggaatccca cggcgcatcc cctgtcaaac gatggatgat gatctctttt ggtatcattt
      901 ccagctggtc gcaaatgaca ttgacatagt caggacggct cattaattga aggcgacctt
      961 catgatagtc ccgcaacatt ctcgttttgc gcatcagatg catcaaatgg agcttgattc
     1021 cttggatatc tgaatctaaa atggtgcgtc tgacattttc tttcatcatt tctaaacttt
     1081 cgcctggtaa tccgttgatc aaatgcgtac aaacacggat attatgtttg cgtaatttag
     1141 ccacgccatc taaataagtt tgataatcat gtgctcggtt gatcagtttt gaagtttctt
     1201 caaaagttgt ttgcaatcca agttctaccc ataaatacat ccgctcgttc agttcagcaa
     1261 ggtaatcaac tacttcatct ggcagacaat ccggtcgtgt accgactgat aatcctacga
     1321 cacctggtaa attgactact tgctcaaatc gttctttgat tatttcaact ggcgcatggg
     1381 tgtttgtaaa attttggaaa taaacgatgt attgttggac atgaggccat ttttgatgca
     1441 tcatgtcgat ttctttctgg aattggattg gaagagggtc ttcaggtgca acgatcatgt
     1501 ctcccgaacc agatacactg caaaaagtac aaccgccatg ggcaacagtc ccatctcgat
     1561 tcggacagtc aaatcctcca tcgatcggca ccttgaaaat tttcccgcca aactcatttc
     1621 gtaaagcgta attccaagaa tgatatcttt tatttccttc tgaataagga aaagtcatta
     1681 aaagtccttc tttctgtaag agttatctat ttgttttctt ttttctctat acactggata
     1741 agtcaggcac agccaagaaa gactgagtaa gtaaccgccg atgatatcag taggaaaatg
     1801 aacgcccaga taaatgcggc ttatcccgac acttactata ataaacatca tcagcaattg
     1861 aagcaatgtg cgtaaggttc ttttttgaac aaaagctgga agcagaaata gtaaagaacc
     1921 gtaaagaatc atactgcctg ctgcatgtcc actagggaaa ctgtaacttg tttcaactac
     1981 taagtgcgcc aatgaaggac gctctcgcat gaaaaatagt ttgatcaccg ggttgatgat
     2041 tccagcaatt acaataaatc cagaaacgaa ccaaaatgtt tctgcccttt ttttcttata
     2101 ccacatccat aaagccgcac aagcaaataa aatgatgacg gtaagaggat taccgaattt
     2161 agtcataaag agttggtagc tgttcagatc aggataagaa gctctgacaa tgcctgtaaa
     2221 ccactgatcg aaaccagaaa gccacgacag atggaacttt acgatccagc ccaaaatgaa
     2281 aaacaggaca agaaagaagc taccagccac ctgaaatgat gttttatatt tcatacaatt
     2341 cctcctttga agcaatgact tattatagca tttttggtcc agcgaaaaaa acggtaaaga
     2401 gataaaacca atgtatgatt cctctttgaa taaccaatat tcttataaaa aaaataactg
     2461 taatcggaaa gacatttttt ggtaatgcaa atgaaatgtt agggaaataa aatcagtgtt
     2521 tttcgaaatc cttttaaaca agaacgtaga cgtaggaaga acttgaaaat gaacagaagt
     2581 tgttcattac ggtaaattac aatatgatta caaaaaacaa caaagctctt aaataacttt
     2641 taataaatat taagagaagt tataattatt aataatagat aaattgaagt tggaggatag
     2701 aagatggaaa acattgcaag aaaagaacgg agacgtttag aacagaccaa acgttttcga
     2761 aaagcaaaac gaggggcagc aatcgtggga actgcgatgg taggctgttc tgtcgctgcc
     2821 ccattagtac agccggttcc tgttcaagcg gatgaaacac ctgttcagtt cggcgcaaga
     2881 atcaatacag cggcatttat cacagaaatt gctacttatg ctcaaccaat cgctcaagcc
     2941 aacgatctat atgcatcagt gatgatcgca caagcagttg tagaaagcgg ttggggcagt
     3001 agtgcgttat ctcaagcacc ttattataat ctttttggga taaaaggcag ctatcaagga
     3061 caaaccgtct acatggatac gcttgaattt ttaaataatc aatgggtaaa taaaaaggag
     3121 cctttccgac aatatccttc ttttgctgaa tctttcaatg acaatgccta tgtacttaga
     3181 aatacttcat ttggcaacgg ttattactat gccggtactt ggaagagcaa cacgaaatcc
     3241 tatacagatg cgacagcttg tttgacaggg cgttatgcga ctgatcctgg ttacgcaggc
     3301 aaattaaata atatcatcgc aacttacgga ttgacccaat atgatacacc agctagcgga
     3361 aacgcaggcg gtggagcgac tacaggtggc aataatactg gaagtgcagg tagtactggc
     3421 ggttcaagca attctggtaa tacaaactca ggttcagcaa cttcgaccac ttatactgtc
     3481 caatctggtg attctgtttg gggaatctca aataaattcg gtatcacaat ggatcaattg
     3541 atccaatgga acaacatcca aaacaatttc atttatccag gacaaaaact gacaatcaaa
     3601 ggtagccagg aaaatggctc ttcgacgaat aattccggta acaataccaa ttcttcagga
     3661 aatgctggta catcaaacgg aggacaaaca actggtgcga aatacactgt tcaatctggt
     3721 gattctgttt ggaagatctc aaatgatcac ggaattacga tggctcaatt gattgaatgg
     3781 aacaatatca agaacaattt cgtttatcca ggacaacagc taatcgtatc aaatggcggt
     3841 tcttctaatg ctggagcaaa tactggctca aatactggga atacaagtgg ctctagttcg
     3901 tccagctcaa gttcttctaa tacagcttca ggaacgaaat atacagttaa agccggagac
     3961 tctgtttgga gcgtctcaaa taaatacgga attacgatgg ctcagctaat ccaatggaac
     4021 aatatccaaa ataactttat ctacccagga caacaactga ctgtttcaaa cagtggatca
     4081 acaaacagca cgtcaggtaa tacaggttcg tcaaccaata caaataacag cagttcagtt
     4141 aatactggca gcagttcgtc tcaaggcact tataccgtga aagcaggtga atctgtttgg
     4201 agcgtggcaa ataaaaacgg catcacgatg gatcagttaa tcgaatggaa taatatcaaa
     4261 aacaacttca tttatccagg acagcaattg atcgttaaag gtggaacttc ggcaaataca
     4321 aatacaggtt caactacttc tgcagcgaaa ccaaatacac cgaatacgtc tgctacgaca
     4381 agtacatcat ccactggtaa tacgatgtat accgtgaaag cgggcgagtc agtttggagc
     4441 gtagcgaaca aacatcatat cacgatggat cagttaatcg aatggaataa tatcaaaaat
     4501 aatttcatct atccaggaca gcaagtgatt gtgaagaaag gttcatctca atcaacaaac
     4561 caacaatcag ttactggttc gaaaacctat actgtgaaat ctggagaatc tgtttggagt
     4621 gttgctgaca gtcatggaat tacaatggct caactaatcg aatggaacaa tattaagaac
     4681 aactttattt atccaggtca aacgttgatt gtagctaaat aaaatagctg ttcgaagaaa
     4741 taacgattga attccacaaa aatatttgtt actataaggc tacttaaata tgacaacact
     4801 tgaaaaggaa gtagtaaaaa gactgaagtt tcagagagcg catggctggt gagaatgtgc
     4861 acggatgctt ttgaactcgc ctttgagttg cccttgctga atacccgagt aagcaagcgg
     4921 cggtgacggc cgttacaacg ttcgaggcct tttcactaag gtaaaattag gtggtaccgc
     4981 gtagattacg tcctataaaa agagcaatct ttttatagga tttttttatt tcttttgtga
     5041 agtttgagaa aacaaagcaa aaggaaataa aaataaacag attctgccgg ttttttcgtt
     5101 atatttataa tagaaacaag tgttttcaat agaacaggag gacagcaaat gagttacaat
     5161 cataaagaaa ttgagaaaaa atggcaaaaa tactgggcaa aaacgaatac tttcaacacc
     5221 catgatgatc ctgaaaagcc aaaattttac gcactggata tgtttcccta tccatccggt
     5281 caagggctac atgtagggca tcctgaaggt tatacggcaa cggatatcct ttcacgtgtg
     5341 aaaagaagtc aaggatacaa cgtgctgcat ccaatggggt gggatgcatt cggtctgcct
     5401 gctgaacaat atgcgctaga tacaggaaac gatccagctg agtttaccca aaaaaatatc
     5461 gaaacattcc gacgccaaat caattcttta ggatttagtt atgactggaa tcgcgaagtg
     5521 aatacaactg atccggagta ttataaatgg acacaatgga tctttacgaa gctttatgaa
     5581 aaaggtctag cttatgaagc agaagtagcg gtaaactggg tacctgaatt aggaacagtt
     5641 atttcaaatg aagaagtgat cgatgggaaa agtgaacgtg gtggctatga tgtagtcaga
     5701 aaaccaatgc gccaatggat gctgaaaatc actgcttatg ctgatcgctt attggatgat
     5761 ctagaattag ttgattggcc agaaagcatt aaagaaatgc aaagaaattg gatcggtcgt
     5821 tcagtaggag caaatattga atttaaagtg gctggtacgg ataaatccta taccgtattt
     5881 acgacacgac cagatactct attcggtgca acttactcgg ttcttgcacc tgaactagat
     5941 cttgttagag aaattacgac ccctgaacaa aaagctgctg tagaagctta tatcgaagag
     6001 acagctaaaa aatcagatct aaagagaacg gatcttgcaa aagaaaaaac aggagtattt
     6061 actggtgctt atgcaatcaa tccagttaat ggcaaggaaa tcccaatctg gatcgccgat
     6121 tatgtattat cttcatacgg cacaggagcc atcatggctg ttccagcaca tgatgaacgt
     6181 gactttgaat tcgctcaaac tttcggacta gaaattcttc cagttatcga aggtggagat
     6241 gttgaaaaag ctgcttacac agaagatggt acgcacatca actctgaatt tctagacggc
     6301 atgaacaaac aagaagcaat cgataaaatg aatgaatggc tagaagaaaa tggcgtaggg
     6361 aaaaaagaag tcagctatcg cttacgtgac tggctattct cccgtcaacg ttactggggt
     6421 gaaccaatcc caatcatcca ttgggaggac ggaacagtca cagctgtccc agaagaagag
     6481 ctaccattac ggttgcctaa gacaaaaaac atcaagccaa gcggtactgg agaatctcca
     6541 ttagcaaata tcgaggagtg ggtcaatgtc gttgatcctg taacaggtaa aaaaggccgt
     6601 cgcgaaacga atacaatgcc acaatgggca ggaagctcat ggtattacct gcgttatatc
     6661 gaccctcata acaaaaaaga actagcagat tatgaaaagt tgaagcgttg gctaccagtt
     6721 gacatctaca ttggtggagc agaacatgcg gtacttcatt tactatatgc acgattctgg
     6781 cataaattct tatacgatat cggtgttgtt cctacaaaag aaccattcca aaaactatac
     6841 aatcaaggaa tgatcttagg cgaaaacaac gaaaaaatgt caaaatctcg tggtaatgta
     6901 gtcaatcctg atgatgtggt cgaaacttac ggtgcagata cactgcgcat gtacgaaatg
     6961 ttcatggggc cattagatgc ttcgattgct tggagcgaaa atggcttaga aggaagccgt
     7021 aaattcttgg atcgtgtatg gcgtttgatc gttgatgaga acaataaaat gcgtgaccgt
     7081 atcactacat tgaatgatgg caagcttgat aaagtgtatc atcaaacagt gaaaaaagtg
     7141 acagaagact atgaaaatct tcatttcaat acagcaatct cccaattgat ggtctttatc
     7201 aatgaagcct ataaagtgga cgctctgcct tatgaatata tcgaaggatt tgttcaacta
     7261 ctcgcaccga ttgctcctca catgggtgaa gaactatggg caatccttgg aaatgatggt
     7321 ggtatttctt acgcaccatg gccaacatac gatgaagcag cacttgttga agacgaagta
     7381 gaagttgttt tccaagtaaa cggtaaagtt cgtgcgaaaa gcaatgttcc ccgagactta
     7441 ggaaaagatg agctagaaaa agcagcatta gccaatgaaa tagtgcaaga atatattgaa
     7501 ggcaaaacag tcagaaaagt tattgcggta ccaaacaagt tagtcaatat cgttgctaat
     7561 taattggctc tttgtcaata aggactgatg agttgtgcaa aatcaaatct gggccagaat
     7621 gaacttcatt ctgtctcaga ttttttgtta tttcaaattg attaattgat tgatcaaaaa
     7681 ggttttgatt ttcatgttct caaacacgtt caatgtcttt ttcttctgaa tggtcttgta
     7741 agagatctct gtaatcatca aatttttgtc tattaatctt agtatttttt gatgtataaa
     7801 ctacctgaaa ttgagagtac ataccaaatt cataaatttt ttattagtgg gactagtgat
     7861 tgtatggatt ttgacgcata atgaatgaag tatgagaaaa ggagagacaa atggatcaag
     7921 aactatttat tatcgaagta ttatcagcta aacaaaaaat tatcgagaaa gcgaaaaaaa
     7981 gagcgaaaag aataatgttc aagcttttaa gcttagtatt tttctctggt ttgctttttt
     8041 tcggagctgt atcgttccta tatcgccata tgagtgcagt agtccgttca gctgaaggga
     8101 tgcaaaaatt aaataatttg tatcactttt cgtggggagt tggcggaata acaattgttg
     8161 tgcttattgt ttttcttcta cttatttggc tccgttacca tcaattgtcg cgtcatttcg
     8221 atcaatggct tagtcaaaga gcacaacagt ttttacataa taaaccaact tctcaagatg
     8281 atgactatct ttattttgag aagacttcga cgagtcaagc ttttcggata caaaaaagtg
     8341 attgccagtt attgctgaga tcacttgatg atttacctat ctacttaggc gaaagcaaat
     8401 caaaattcgg aatgaacaca ttccatattt ttttactaga aaacgaaccc agatgggaaa
     8461 aaagccagtc ttcgcctggt ggatacctga caactaagat gagtacatta gtcactggtt
     8521 ttttcctcat tcttttagct ggaacaatct attatgggag atacactcag ccaaaaatgt
     8581 cttctcatat aaatgctgct tctcacatga caatgaactc aactaacgat acgcatatac
     8641 aaaagacaaa tgaagggttg ttagctgatc ctaagcaaaa ggtaaatttg acaacacaaa
     8701 agataaatga tctcaaactt gatacagaga cgcactctct ctatatgaca acagatagtg
     8761 gtgctaattg ggtatttgtg ccgattgatt cgcaatggtt gcgttttggc gaatacaccc
     8821 tgacaacggg gctgattcct gaaggttatt ggatggacaa cacatatgat atttccgccg
     8881 atttctcatg gtttatctat tcttctgggg atgatgtacg tctgctgact tcaagagata
     8941 atggtaaaac atggcaaacg tctattatca cccaatcagt tcagccgata cgttatcgaa
     9001 aagcggtatt ttatggcaac gatggtctat ttatctgtac atcagccagt gggatgtctg
     9061 cagaaacttt gtacatttac actactatgg atggcggaat cacttggata aaaagtggaa
     9121 gtacaatgat caatcagcca gtacaaaatg tcagcttcgt tacaacttct ttaggttttg
     9181 tttcgacacg tgaaagtttg ttttatacaa acaacggagg acgctcattc aaagaatcag
     9241 ttctgtcgat tccggaaaac tatcagacag gggggataga tctttttcaa tcaccaaatg
     9301 aagttactca aataagcgcg aatacgttag agacaaaatt ttatttgttg aaaaaaggac
     9361 caatcgatgc agggaaaatg tttgcctgcc tcttccgttc aactgataac ggggaaacat
     9421 ggcaattcgt tcagcaacta tcgcaagtag aacaaggaga ttaaagaaga gagattgtga
     9481 gcctgacgtt cagcaccgag tcacaagaaa caaattcaaa aaacagcttc tcatatttgt
     9541 tgaatagtgt aactcgtgat tcggagttga caagagcaac aagaagcgat tttgaagtat
     9601 agcttctcgt attttacaag atcgtgtctt attgccgata ggttgtctct tgaacaccgt
     9661 ttattcgtaa aaagggattg tgacaagtct tttgtcacta cttaccgaac gaaactgaag
     9721 gagctatcca aaaagtatca ttagtattat tctttgtgtt tctagtggca ccactagtac
     9781 atttaaaaaa gctatcgatc atggactaat catacatgat cgatagcttt ttagataatg
     9841 tttgttacgc tatctgaaat tgactattat aaagactggc ataaagcgtt ggtgattgga
     9901 gtagttcaga atgtgttcct ttttcgacga tatcgccatt cttcattaca aggatcatgt
     9961 ctgcattttc aatcgttgac aaacggtgtg caatcacaaa acttgtccta tcttttgtca
    10021 cagcttccat tgcttgctga atcaatgctt ctgttcgtgt atctacgcta gaagttgctt
    10081 catctaaaat aacgacagga ggattcgcta gaatgactcg agcaattgtc aataattgtt
    10141 gctgtccttg agataacgtg ccgttttcgc tagaaatgat cgtatcatat ccgtctggca
    10201 gtgttcgaat aaagtgatca cattgtgcga tttcagccgc ttcgataatt tgttctcttg
    10261 tggcatccat tttcccgtag gcgatattat cagctaccgt tccttcaaac agccaggtat
    10321 tttgtaaaac catcccaaaa gaacgacgta gttctccacg tgaaagcttg gtaatatcaa
    10381 gaccgtcgaa ttcgatcttt cctttattca gttcataaaa acgcatcagc agattgacta
    10441 aagtcgtttt tcctgcacca gttggaccaa cgatcgctac cgtttgtttt ggctttactg
    10501 aaaaatcgac ttgattcatc aatattttat ctgatgtgta accaaactgt acatttttga
    10561 aagctatttt tcctttcggt tgttctaaaa gtcttgcatc tggaaggtct ggaatttcat
    10621 ctgtttcatc taatatctca aatatcctat cgattgctgc cattgcggat tggatcgagt
    10681 tgatcacata agaagcggta gaaattggtt cagaaatctg attgatgtac tgaagataag
    10741 cttgtaagaa gccgatcgtc acaccacctg atagaacaag catagcaccg aataccgcac
    10801 ttatgatgaa ggcgagttga ttaatgaaac gaatggccgg atagatagca cagttcataa
    10861 attgagcacg tttaaaagcg ttgtattgct ttttatttac ttcgtgtata gcagcttctg
    10921 catgagcttg ctgattgaat gttttgacca ctagattacc agctaagatt tcttccattc
    10981 gattatttaa aacacccagt tcatactgat tctgttcaga aatctgtttg tttttgttcg
    11041 caatcttagt tgtaacaatt gaactgccaa cgatcagcaa taagacgagc aaagtaagct
    11101 tgctttcgat ataaaacagc atcagaccag cagcagctat tgttacgaca gaagtaaaaa
    11161 attgattgat acctgtcaat aacacttgag aaagttgatt gagaccggta gttgtccgac
    11221 taatgatgtc acctacttga tgattgtcaa aaaacgacat cggtaatgtt ttgaattttt
    11281 ttgtgatttc ttttcgcacc ctcaatgtga tcttttcgct taacgctgcc attgtccgtt
    11341 cctgaataaa tgaagtgatg ctgcttaaga ctgaacagcc gatcagcaat aagacaggga
    11401 acaacaatgc ttgtttgata tcactgaaag aaagctgtcc aatacctgtt tttcttattt
    11461 ctgataagag atcatcgatc ccgattccca tgatcaaggg catggctacg actaataggt
    11521 tcccgaataa actgcataga aacaatccgt aaaaatattt atgttcaggt ttgatcattt
    11581 tccaaaaacg tatcaaagca cgcgtgttca acgttttttt cttttccatc gtatcccttc
    11641 tttctatatt ccttgtgatt ttgcaaactc ctgataagtt tggcaattag acagtagttc
    11701 ttcatgagta ccttgtccaa cgatttttcc ttcatctaaa acaatgatgt tatctgcttg
    11761 ttggattgta ctgaggcgtt gagcaacaat cacgagagtt ttctccgaca tgtgtttatg
    11821 aagggctgct cttaatgctg catctgtttt atagtctaaa gcggagaaac tatcatcgaa
    11881 tacatagatc tctgctggtt tgatcaacgc tcgagcaata catatccgct gtttctggcc
    11941 ccctgaataa ttagaaccgc cttgagcaac aaagctgtcc aatccgtcag gaagggtgtc
    12001 cactagttct tctaattgag cgatcttgat cgctttagac aactcttctg ttgaggcata
    12061 ttcatttccc atctgcaagt tgcttaaaat tgtcccgctg aataagaaag ccttctgagg
    12121 gacataactg atgtgggagc ggatctgttc ttgagtcatt tgagtaagag gaataccacc
    12181 aagggttact tttcctatcg tagggtcact aagtctcaag agaagtttcg ccaaactact
    12241 ttttccagat ccggtcccgc caacgatcgc tgttgttttt cctttaggta ttgtgaaatc
    12301 aatatcttct agaactggat cagagatttc agtatattta aatgtgacat gttcaaaagc
    12361 agctgcattt gtctgatcaa aggttggttc agctactgtt ttgatcgtct cttcctcaat
    12421 ttcgatctct gcttgcagta tttcttctat ccgtcgtaat gaggcaaacg atttagggat
    12481 aatgaccaat accatagctg cgatcatcaa gtaagcaagt gtcagcatag agtattcgac
    12541 tacagcagtc acgacaccga tttgtaatgc tccttctgct acaagatttc cgccaaacca
    12601 caaaatagaa gcataaacga ttcccatgag caaaaaagca attggagtga cccaagcaaa
    12661 tattcggttc actttgatca tttgattagc atagtcagca aagctcttat tcgttcgctt
    12721 ttcttcatat gattgattat caaaggcacg gatcatatta atcccagtaa agaattctct
    12781 caatgtgatg gtgatccgat ccatttttgg ctggatactc aaagataaag gtgcccctcg
    12841 tttcatcagt cgataaacaa tccatacata aaaaacaaca gagatcaatg taacccatgt
    12901 tagtaataga gaataacgaa acatcatgag aatggtgaaa acgcaaataa ccggtgctgg
    12961 taagatcatt tggcaaaata aaacaatcat ttgctggaca ttatcgacat cattcgtcat
    13021 tcttgtcaaa agagatccag aactaaaacg actgatatcc ttcattgaca gctgttggaa
    13081 tttacggaaa tatttctccc gcgtttggaa tccaaaccta gcggaagcca atgctgaaaa
    13141 gtaactagag gcaactgcag cgattgttcc tagaaacgct acaagcagca tttcaactcc
    13201 aatttttttt ataactgagg cttcgccact tgctatacct gtgtcgatca gctttgctac
    13261 taattttggt acacctaatg taccggcgat ttctaaacag atcatcacaa aggtaaggag
    13321 agcgagtcct ttgtttttct gtaagaatgt ttttaagata gacatattat tcctcctttt
    13381 ttcctgatac ttttcgattc tataccttca agtaacttga aggtcaatag aaaaaagaat
    13441 aaactgaaaa agaggtgaga aaaaatgaaa aaaatgtata cgattggcca agtatcggaa
    13501 ctatttgatt tgccaaaatc aacgatccgc tattgggatg aacaaggatt aatccgttca
    13561 agtagacaag aagagaatga ttatcgattg tttgatatcg atgatatttt tatgctttat
    13621 gatatcgttt tttatagaaa attagatata ccgatcaaac agatgaagaa tttatatgga
    13681 aaaacattga cggagttata tgagacactt gatgaaacag aaagaagaat ccataaagaa
    13741 ctggtggtga tgaaacaaaa acaaaaagag ataagggagc aaaaaaaaca gttgaaattg
    13801 atgatcgata caaacgagga agaatttcct gtagaagaga tcccttttga ctgtatgatc
    13861 tcaacggagt ttgaagatat tgtggaaatc aaaaaatttc tacctaacta ttcaagcttt
    13921 gggatgatga gtatgccagc ttccagttca cagacgatgt atggattttt tatcgaccct
    13981 agtgaagtac atttatttac tcaagacgtg atttgggaga aaaaagatac ggctgtttat
    14041 cgtcgattct tattgaaatc cgagatgaac catgcggaac gtaataatat tttagaaatc
    14101 agagaaagaa tgtatgaaaa aggatggaaa acaggagaag tgatcggcca gtatctattg
    14161 acgaatacgg atgaaaacaa tatcagaact gaatattatc atgcatggat agaaatgaaa
    14221 aaataggaaa tatgtgttta agataactcg aaaatgatgg ttgttttagt tataaagaaa
    14281 aattttctgg ttcgattacg ggtttaatgt gggggattat tagtcgtgga tagatagaat
    14341 tttagttttt tagaggaaat atgtctttaa aaatggaatg gttgcaattc ttagcgatat
    14401 atacgatgta attgtcctct taaaaaagta tggaaaaata attgtattgg gtgttatgta
    14461 taattcatgg tgaactgaga ctaatgagaa gaaaaattca tattgttatg agtgagattg
    14521 tatcagatga gaatgataaa ttaaataggt gcataaaagc attacactta aaatacattg
    14581 ttgcttacat tattacaat
//
LOCUS       DARBER010000055        14497 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DARBER010000055 DARBER010000000
VERSION     DARBER010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.056"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(130..825)
                     /locus_tag="SCK06_001599"
     CDS             complement(130..825)
                     /locus_tag="SCK06_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG1151602.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="SCK06_001600"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="SCK06_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG1151603.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="SCK06_001601"
     CDS             complement(2024..2215)
                     /locus_tag="SCK06_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG1151604.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="SCK06_001602"
     CDS             complement(2236..3126)
                     /locus_tag="SCK06_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG1151605.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="SCK06_001603"
     CDS             complement(3113..3880)
                     /locus_tag="SCK06_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1151606.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="SCK06_001604"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="SCK06_001604"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG1151607.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="SCK06_001605"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="SCK06_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG1151608.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="SCK06_001606"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="SCK06_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG1151609.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8451..8834
                     /locus_tag="SCK06_001607"
     CDS             8451..8834
                     /locus_tag="SCK06_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151610.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="SCK06_001608"
     CDS             complement(9002..9760)
                     /locus_tag="SCK06_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG1151611.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="SCK06_001609"
     CDS             complement(9778..10596)
                     /locus_tag="SCK06_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1151612.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="SCK06_001610"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="SCK06_001610"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG1151613.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="SCK06_001611"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="SCK06_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG1151614.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="SCK06_001612"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="SCK06_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG1151615.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="SCK06_001613"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="SCK06_001613"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG1151616.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         4023 a         3053 c         2593 g         4828 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaa
//
LOCUS       DARBER010000056        14426 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARBER010000056 DARBER010000000
VERSION     DARBER010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.057"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..335)
                     /locus_tag="SCK06_001614"
     CDS             complement(6..335)
                     /locus_tag="SCK06_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG1151617.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            512..1240
                     /gene="sfsA"
                     /locus_tag="SCK06_001615"
     CDS             512..1240
                     /gene="sfsA"
                     /locus_tag="SCK06_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG1151618.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1256..2269
                     /locus_tag="SCK06_001616"
     CDS             1256..2269
                     /locus_tag="SCK06_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1151619.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2269..3546
                     /locus_tag="SCK06_001617"
     CDS             2269..3546
                     /locus_tag="SCK06_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1151620.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3609..6311
                     /locus_tag="SCK06_001618"
     CDS             3609..6311
                     /locus_tag="SCK06_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1151621.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6463..6780
                     /locus_tag="SCK06_001619"
     CDS             6463..6780
                     /locus_tag="SCK06_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1151622.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6810..7130
                     /locus_tag="SCK06_001620"
     CDS             6810..7130
                     /locus_tag="SCK06_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1151623.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7238..8698
                     /locus_tag="SCK06_001621"
     CDS             7238..8698
                     /locus_tag="SCK06_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1151624.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8766..8987
                     /locus_tag="SCK06_001622"
     CDS             8766..8987
                     /locus_tag="SCK06_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151625.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9018..9200
                     /locus_tag="SCK06_001623"
     CDS             9018..9200
                     /locus_tag="SCK06_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG1151626.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9200..9613
                     /locus_tag="SCK06_001624"
     CDS             9200..9613
                     /locus_tag="SCK06_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG1151627.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9736..10917
                     /locus_tag="SCK06_001625"
     CDS             9736..10917
                     /locus_tag="SCK06_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151628.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10988..11152)
                     /locus_tag="SCK06_001626"
     CDS             complement(10988..11152)
                     /locus_tag="SCK06_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151629.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11306..11379
                     /locus_tag="SCK06_001627"
     tRNA            11306..11379
                     /locus_tag="SCK06_001627"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11340..11342,aa:Arg,seq:cct)
     gene            complement(11448..12587)
                     /locus_tag="SCK06_001628"
     CDS             complement(11448..12587)
                     /locus_tag="SCK06_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1151630.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(12886..13521)
                     /locus_tag="SCK06_001629"
     CDS             complement(12886..13521)
                     /locus_tag="SCK06_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151631.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13634..14269)
                     /locus_tag="SCK06_001630"
     CDS             complement(13634..14269)
                     /locus_tag="SCK06_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEG1151632.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(14303..>14426)
                     /locus_tag="SCK06_001631"
     CDS             complement(14303..>14426)
                     /locus_tag="SCK06_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEG1151633.1"
                     /translation="NSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT         4621 a         2436 c         2860 g         4509 t
ORIGIN      
        1 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
       61 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
      121 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
      181 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
      241 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
      301 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
      361 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
      421 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
      481 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
      541 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
      601 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
      661 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
      721 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
      781 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
      841 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
      901 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
      961 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
     1021 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
     1081 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
     1141 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
     1201 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
     1261 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
     1321 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
     1381 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
     1441 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
     1501 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
     1561 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
     1621 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
     1681 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
     1741 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg
     1801 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
     1861 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
     1921 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
     1981 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
     2041 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
     2101 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
     2161 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
     2221 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
     2281 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
     2341 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
     2401 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
     2461 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
     2521 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
     2581 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
     2641 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
     2701 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
     2761 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
     2821 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
     2881 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
     2941 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
     3001 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
     3061 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
     3121 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
     3181 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
     3241 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
     3301 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
     3361 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
     3421 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
     3481 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
     3541 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
     3601 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
     3661 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
     3721 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
     3781 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
     3841 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
     3901 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
     3961 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
     4021 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc
     4081 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
     4141 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
     4201 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
     4261 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
     4321 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
     4381 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
     4441 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
     4501 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
     4561 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
     4621 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
     4681 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
     4741 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
     4801 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
     4861 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
     4921 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
     4981 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
     5041 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
     5101 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
     5161 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
     5221 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
     5281 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
     5341 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
     5401 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
     5461 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
     5521 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
     5581 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
     5641 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
     5701 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
     5761 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
     5821 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
     5881 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
     5941 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
     6001 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
     6061 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
     6121 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
     6181 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
     6241 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
     6301 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
     6361 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
     6421 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
     6481 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
     6541 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
     6601 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
     6661 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
     6721 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
     6781 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
     6841 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
     6901 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
     6961 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
     7021 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
     7081 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
     7141 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
     7201 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
     7261 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
     7321 gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
     7381 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
     7441 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
     7501 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
     7561 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
     7621 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
     7681 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
     7741 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
     7801 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
     7861 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
     7921 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
     7981 gcaactgatc attgatttgg tgcaaaaata tattgcagag ccattcctag gattatctca
     8041 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
     8101 tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
     8161 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
     8221 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
     8281 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
     8341 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
     8401 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
     8461 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
     8521 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
     8581 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
     8641 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
     8701 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
     8761 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
     8821 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
     8881 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
     8941 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
     9001 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
     9061 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
     9121 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
     9181 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
     9241 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
     9301 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
     9361 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
     9421 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
     9481 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
     9541 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
     9601 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
     9661 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
     9721 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
     9781 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
     9841 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
     9901 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
     9961 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    10021 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    10081 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    10141 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    10201 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    10261 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    10321 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    10381 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
    10441 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
    10501 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
    10561 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
    10621 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
    10681 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
    10741 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
    10801 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
    10861 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
    10921 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
    10981 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
    11041 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
    11101 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
    11161 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
    11221 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
    11281 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
    11341 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct
    11401 ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt
    11461 ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga
    11521 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
    11581 acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca
    11641 tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa atatttgctg
    11701 acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt
    11761 ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt taagagatca
    11821 atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt ttttccccat
    11881 tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc gaaatcgata
    11941 tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc agcaactaac
    12001 acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt cattaaagat
    12061 ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc ttttcctttg
    12121 attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc atctttcaac
    12181 gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc tttagcatac
    12241 ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac cgctggcata
    12301 tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg agatactgtt
    12361 ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag atttttgcga
    12421 tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc ttttgcttct
    12481 ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat
    12541 gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat tgaatcagct
    12601 ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt tattattttt
    12661 tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta atggtataat
    12721 tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg atttttaaaa
    12781 gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc tggtttttta
    12841 ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag taagagttga
    12901 gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt tgccgacatc
    12961 acctaatact atggagctaa tcgttttttc taacagacca aaatcagata atctatttaa
    13021 gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag tgaaattttc
    13081 gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta attgattcag
    13141 tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa gtaaacaatt
    13201 cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt ctacctcgtc
    13261 atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga aaacatcttc
    13321 tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga atttctcgat
    13381 gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa cgccaccaat
    13441 ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt ttttggttag
    13501 tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta caagcattgt
    13561 gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta actcttagtg
    13621 gggcgtggtt catttatttt ttacttactt tactattaat catatcccat tgagcatagg
    13681 atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact ttatcgccct
    13741 ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa actttaggtg
    13801 ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata atggctattc
    13861 tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca ctaatatatt
    13921 tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta tctaagaaag
    13981 caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg cttttattgc
    14041 tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg ttattgtcat
    14101 ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc ccgataataa
    14161 ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt aacatgatta
    14221 aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt aatagatcct
    14281 ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta aaatcgaatt
    14341 cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt tcatctgcac
    14401 ttgaaatttg tgtttctttg gaatta
//
LOCUS       DARBER010000057        14344 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARBER010000057 DARBER010000000
VERSION     DARBER010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.058"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            110..491
                     /locus_tag="SCK06_001632"
                     /pseudo
     CDS             110..491
                     /locus_tag="SCK06_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            622..1572
                     /locus_tag="SCK06_001633"
     CDS             622..1572
                     /locus_tag="SCK06_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1151634.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2545
                     /locus_tag="SCK06_001634"
     CDS             2090..2545
                     /locus_tag="SCK06_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1151635.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2886..3488
                     /locus_tag="SCK06_001635"
     CDS             2886..3488
                     /locus_tag="SCK06_001635"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151636.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3582..3914
                     /locus_tag="SCK06_001636"
     CDS             3582..3914
                     /locus_tag="SCK06_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEG1151637.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4065..5044)
                     /locus_tag="SCK06_001637"
                     /pseudo
     CDS             complement(4065..5044)
                     /locus_tag="SCK06_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5049..5816)
                     /locus_tag="SCK06_001638"
     CDS             complement(5049..5816)
                     /locus_tag="SCK06_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1151638.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="SCK06_001639"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="SCK06_001639"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEG1151639.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..7263
                     /gene="lacB"
                     /locus_tag="SCK06_001640"
     CDS             6748..7263
                     /gene="lacB"
                     /locus_tag="SCK06_001640"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEG1151640.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7274..8206
                     /gene="lacC"
                     /locus_tag="SCK06_001641"
     CDS             7274..8206
                     /gene="lacC"
                     /locus_tag="SCK06_001641"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEG1151641.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8209..9189
                     /gene="lacD"
                     /locus_tag="SCK06_001642"
     CDS             8209..9189
                     /gene="lacD"
                     /locus_tag="SCK06_001642"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1151642.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9217..9534
                     /locus_tag="SCK06_001643"
     CDS             9217..9534
                     /locus_tag="SCK06_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1151643.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9534..11240
                     /locus_tag="SCK06_001644"
     CDS             9534..11240
                     /locus_tag="SCK06_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1151644.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11382..12788
                     /gene="lacG"
                     /locus_tag="SCK06_001645"
     CDS             11382..12788
                     /gene="lacG"
                     /locus_tag="SCK06_001645"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1151645.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12811..13083
                     /locus_tag="SCK06_001646"
     CDS             12811..13083
                     /locus_tag="SCK06_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEG1151646.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13104..14003
                     /locus_tag="SCK06_001647"
     CDS             13104..14003
                     /locus_tag="SCK06_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1151647.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4686 a         2363 c         2765 g         4530 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt
     6961 aacaatgcag tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca
     7021 gctatatatg caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact
     7081 ggtggtcttc ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca
     7141 gaagaaaata aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa
     7201 gcagatgaag atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat
     7261 taaggtataa caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc
     7321 tctagaaaca ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg
     7381 tggaaaaggc ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac
     7441 aggtttctta ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt
     7501 tagtccagct ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga
     7561 gggtaaccaa acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg
     7621 ctttcttgac cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag
     7681 tttaccttca ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga
     7741 aggagtagca gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc
     7801 ggctaaacct actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga
     7861 agtaacaaaa gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat
     7921 cgaatggata gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt
     7981 ctataaggta gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac
     8041 ggtagctggt attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca
     8101 tgcgatgaca ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac
     8161 taattacgaa acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca
     8221 gaacagaaac gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg
     8281 gcatttgacc aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca
     8341 actgtagctc aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct
     8401 tcttcaatgt tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa
     8461 gcaggtcttc ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg
     8521 cctgactgct tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt
     8581 aagttcttgc tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct
     8641 tatattgaac gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc
     8701 cttgcttatg atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct
     8761 cgcaaagtca ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt
     8821 aaagtagaag taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt
     8881 tacagtaaag ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca
     8941 tacatctacc taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct
     9001 cacgactcag gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct
     9061 gtagaacctt acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga
     9121 ttcgaaaata ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat
     9181 aaagtatagt aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc
     9241 ttagggtttg aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt
     9301 aaagcggctg aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc
     9361 tgtattgcag aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag
     9421 gaacttccat acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta
     9481 ttaaaagatg tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata
     9541 aactcattga acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct
     9601 atcttcgtgc tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta
     9661 tctttatcct tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg
     9721 agaccttctt gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg
     9781 gtactaccgc taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga
     9841 ttaacttctt atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc
     9901 ctgcaaaaga aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat
     9961 ttattgcagc ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta
    10021 ttcgtatgcc tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat
    10081 tcactgtagc ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg
    10141 taaccgttgc agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt
    10201 accttggaat tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg
    10261 gtccttcaat tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact
    10321 tgcatcttat ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt
    10381 ttattgttac catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga
    10441 tttgtaaatc agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg
    10501 gggttaatga gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac
    10561 cgttcatttt cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga
    10621 acatgaactc attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac
    10681 tcggtactaa cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata
    10741 ctatcattta ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt
    10801 ctggtaaaac aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag
    10861 cagatgccgt tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata
    10921 tcacaaaaga aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg
    10981 ccaatgcctt gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta
    11041 gttacggtgc tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg
    11101 tcgcttcaaa cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa
    11161 aaactgaagg agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg
    11221 ttcaacaaca gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca
    11281 attccttata atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca
    11341 gaatatcgtc tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta
    11401 aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg
    11461 atggtaaagg cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag
    11521 agcctgcaag tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg
    11581 gcgttaatgg tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg
    11641 aagtaaatga aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc
    11701 atgttgagcc attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg
    11761 gagatttctt aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg
    11821 aagagttccc agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg
    11881 gtcaatactt ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc
    11941 aatcacacca caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag
    12001 gttataaagg cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag
    12061 aaaatccagc agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct
    12121 tggatgcaac ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc
    12181 tagctgaaaa tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag
    12241 ctaaagattt gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct
    12301 ttgatggtga gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc
    12361 agattaaagg tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact
    12421 ggattattta tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa
    12481 actacaagaa gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata
    12541 ataccgttta tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag
    12601 acgcaattgc ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct
    12661 tctcatggtc aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc
    12721 aagaacgcta tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga
    12781 tagagtaata ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa
    12841 cacttattta gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg
    12901 attcctgagc ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga
    12961 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
    13021 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
    13081 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
    13141 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
    13201 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
    13261 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc
    13321 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
    13381 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
    13441 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
    13501 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata
    13561 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
    13621 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
    13681 cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca
    13741 ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
    13801 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
    13861 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
    13921 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
    13981 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat
    14041 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
    14101 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac
    14161 agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc
    14221 attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga
    14281 actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata
    14341 gcgt
//
LOCUS       DARBER010000058        14246 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARBER010000058 DARBER010000000
VERSION     DARBER010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.059"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(50..412)
                     /locus_tag="SCK06_001648"
     CDS             complement(50..412)
                     /locus_tag="SCK06_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151648.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(486..866)
                     /locus_tag="SCK06_001649"
     CDS             complement(486..866)
                     /locus_tag="SCK06_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG1151649.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            930..2123
                     /locus_tag="SCK06_001650"
     CDS             930..2123
                     /locus_tag="SCK06_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1151650.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2200..2784)
                     /locus_tag="SCK06_001651"
     CDS             complement(2200..2784)
                     /locus_tag="SCK06_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1151651.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2985..3983
                     /locus_tag="SCK06_001652"
     CDS             2985..3983
                     /locus_tag="SCK06_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1151652.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4095..4604
                     /locus_tag="SCK06_001653"
     CDS             4095..4604
                     /locus_tag="SCK06_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG1151653.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4634..5293)
                     /locus_tag="SCK06_001654"
     CDS             complement(4634..5293)
                     /locus_tag="SCK06_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG1151654.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5295..6059)
                     /locus_tag="SCK06_001655"
     CDS             complement(5295..6059)
                     /locus_tag="SCK06_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG1151655.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6067..6744)
                     /locus_tag="SCK06_001656"
     CDS             complement(6067..6744)
                     /locus_tag="SCK06_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG1151656.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6781..8166)
                     /locus_tag="SCK06_001657"
     CDS             complement(6781..8166)
                     /locus_tag="SCK06_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1151657.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8376..8460)
                     /gene="ffs"
                     /locus_tag="SCK06_001658"
     ncRNA           complement(8376..8460)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SCK06_001658"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8483..8998)
                     /gene="tadA"
                     /locus_tag="SCK06_001659"
     CDS             complement(8483..8998)
                     /gene="tadA"
                     /locus_tag="SCK06_001659"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG1151658.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9105..9962
                     /locus_tag="SCK06_001660"
     CDS             9105..9962
                     /locus_tag="SCK06_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1151659.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10043..10378)
                     /locus_tag="SCK06_001661"
     CDS             complement(10043..10378)
                     /locus_tag="SCK06_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151660.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10445..11815)
                     /locus_tag="SCK06_001662"
     CDS             complement(10445..11815)
                     /locus_tag="SCK06_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG1151661.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11893..12429)
                     /locus_tag="SCK06_001663"
     CDS             complement(11893..12429)
                     /locus_tag="SCK06_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1151662.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12738..14219
                     /locus_tag="SCK06_001664"
     CDS             12738..14219
                     /locus_tag="SCK06_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1151663.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4376 a         2850 c         2330 g         4690 t
ORIGIN      
        1 aaagaagttt gaaaaaaatt caaacttctt tgttcgctta atctgtattt tagaagaaag
       61 gcactccctc tacttcgtaa gttccaagaa cagagcgcat ttgacctggt tcttcccaaa
      121 tatatccttc gcctaattct tctctttgat tcttttgttc agtttcaaag acaaacaacg
      181 gaacattttc ctctttaata tgtgtatttg tcaattccat caaactaatc ttgctaacat
      241 cgatatgaac tatttctttt aagaaattaa gtatattcgc taaaccagtc tttccttcag
      301 aaagttccgc taacgcaaat tccaacttgt catcaattga atggaccaaa aattttttag
      361 taccatcctc caaatgcaac ataattgtac cagcaactct tttgcttgcc aaaatttcca
      421 cctcgatcaa cttgttctat ggcttacata aaaattctag cacattttca gaaaaaatac
      481 actttttatt tcttattttc tttccgttct agttcagata atgcttcttc tatccaaata
      541 ggtaactgct ccgcaatagt ggtaaatccg atcttacgat ttttattcgt gaaatacctt
      601 tttctgcgat atggaacagc tggatttgtt ttttctaacg ttcttaaaga taaactgatt
      661 tttttggaat attcatcaat gtcgatgatc tgaactgtta ctggctgtcc gacttttaac
      721 aatgaatgga tatttttcgt ataaccggat tgtacttctg aaacatgaat aagtccttgc
      781 gtctcgttat caagagaaac gaatgcgcca taaggctgta tccctgtcac ttttccttca
      841 attatttgtc caattttata agtcatctac ttccctctct tcgcatttat cattatactg
      901 taaatagctc aaaaaaagaa gcgacgaaaa tgactggagg tcttaagatg atcgaatttg
      961 ataaacagat ttcaagaaaa gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat
     1021 atcaaatgga tgacttactt cctttgtggg tagcagatat ggacttcttg tctcccggtg
     1081 gggtagctaa agcgtttagt gaatacatca aacatggaat ctttggttat gccactctgt
     1141 ctgataaact atatcaggct atcattcatt gggaacgcca acaccatgct gtggagttga
     1201 cgaaagaaaa tatcgttttt acaagtggtg tgttaaccag tttagctgca gcagtacaaa
     1261 cttttacaaa accaggcgat tctattttga ttcatgaccc agtttatcct ccattttcct
     1321 ctattataga aacaaatcaa cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc
     1381 atttcatcat ggacttgtct gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa
     1441 tgattttatg caatcctcac aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac
     1501 agttaagcga attgtgttta aaacatcaag tgattctgtt tagtgacgag atccatcaag
     1561 atttaacttt attcgatcat acatttactt ccatgctaac gattgatcct aatttagatg
     1621 atctactagt tgcttttact tccgcaacca agactttcaa tctagctgca atcaaaaatt
     1681 caatggtatt tatcaaaaat ccagagatga aagctgattt tgaaaaacat ttagtgatga
     1741 accaacagca cgaaatcaac acttttggac tgattggaac ccaagcggct tatgagacgg
     1801 gtggagaatg gttaagccaa ctgattcctt atctagaaaa aaatgttcaa acagttaatc
     1861 agttttttga acagtatctt ccgaaagtga gaataatgaa ccctgaagga acttacttaa
     1921 tgtggttaga ttttaccgcc tatatttcag acgaccaaga attggagaac actcttgttc
     1981 ggaaaggaaa agtcgtatta aatcctggca tcacgtttgg tccttccggt catgggcata
     2041 tgcgcttgaa tattgcttgt ccagaagaaa ctttactgga aggacttaat cgaatcaagt
     2101 atgctttaga gacaatcgat taaatcattt attcaaaata aaaggacgag accttactag
     2161 gtttcgtcct ttttcataat tactccgtac cataccaaac tattctgaaa tttcaattgt
     2221 ttcaatcact acatcatgta caggtcggtc ttgtggacca cgttgtacat ttgcgatttc
     2281 atctactgtg tccatccctt ctagtacatg tccaaagact gtatgtcgga aatctagcca
     2341 aggagttccg ccacctttat acgcttcaat gatttctgat gggaatccag caccttctag
     2401 ttgtccaagc atattagctg gcacatttgt gttatttaca ataaagaatt ggcttccgtt
     2461 tgtgtttgga cctgaatttg ccatagataa agctccgcga aggttgaaca gttcacgact
     2521 gaattcatct tcaaaacttt cgccataaat actttcgcca cccattcctg ttccagttgg
     2581 atcgccgcct tggatcatga aatcaggaat aacgcggtga aaaatcacgc catcataata
     2641 accttttttg gaaagttcaa tgaaattttg aaccgtcttc ggtgctagtt cagggaataa
     2701 ttgaacagta atgtcaccac ggtttgtttt gataacagct tttggtcctt tttcactttt
     2761 taaatctagt tgtgggaatg ccatattgtt tcctcctaat atttgcatac ttcataataa
     2821 taataacata atacctaacc tctcgtaaaa atactagatt tgttttcttt tatttttcat
     2881 cactagatac attctataac ttaccgtttc gatttcgaat tcaaacattt ttgagaagca
     2941 ttctcaatta aattctgcta tagtgtgaaa agggaggaat ttttatggaa atctatgaca
     3001 ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc
     3061 aagctaaaac aaaaatcatt gacagtctac ctcaacttgg tggtcaatta gcaactttgt
     3121 atcccgaaaa atatatttac gatattcctg ggtatccagc aataaaagca agtgaattaa
     3181 tcgatcaatt agaaaaacaa ttaactactt ttaaccatac attccattta aaagaagaag
     3241 ttctctcgct tactcgtgaa gacgaagtta tcgagattac tacaaataaa ggcatccatt
     3301 attccaaagc agtcatttta gctttaggaa atggttcatt ccagccaaga aaattgaatt
     3361 tagataatgc tgaatcattt gaaaaccacg gattggatta tttcgtgaat gatttgatga
     3421 agtatgctgg aaaaaaagtc gctattgctg gaggtggtga ttctgctatc gattggacgt
     3481 tgatgttaga gcctattgca agcgaagttt acttgattca tcgtcgcccg gaatttcgtg
     3541 cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt caacttgctt actccctatc
     3601 tcatcgatgg attatcaggc aataatgggg aactgacaga tatccggtta aaaaaagtga
     3661 aaagtgacga gacgattgat ttgatgattg attctctgat cgtcaattat ggattctctt
     3721 ctaatttaga acatctttct tcttggggac ttgatagtac cagaaatgcc attactgtaa
     3781 aatcagatat gtctacttct attccaggcg tttatgcagc tggtgacatc tgtacctatg
     3841 aaggaaaagt gaaactgatt gctactgggc tcggtgaagc acctactgca gtgaacaacg
     3901 ctttacacta tattaatcca aaggaacgaa ctcagcctgg tcacagcaca agcttatacg
     3961 ataaaaatct tcgtcctagt tgatttttta tatctctttg catttcaagt taaacccgaa
     4021 agcagtttct tgcatttttt tcatgttccg ttaacatgaa atagagaaag aaaaaataaa
     4081 gggggagcaa taatatggtc gtcaaaactg agactttaga agaaaaggca agagaactat
     4141 tgaaggaacg tggcgtaact gtagaagata tcgcacaact tgtcatgttt ttacaaaaag
     4201 actatatttc tgatttgact ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa
     4261 agcgtgaagt acataacgcc attatcactg gaatccaatt agatatatta gcagaaaaga
     4321 aaaaattgat gagtccgttg caacatatta tcgaagatga cgaaggcctt tacggtatcg
     4381 atgagatcct agccttgtca atcgtgaatg tttacggtac gatcggcttt acgaattatg
     4441 gttatatcga caaggtgaaa cctggtattt taaaagaact aaacagccac gatggcgttc
     4501 atgtccatac cttcttagat gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat
     4561 tagcacacaa agaaccagaa aaaagaagtc gccttactca ataattcaac aaaaaaagaa
     4621 agaaaacgtc tatttagacg agttctttct tttttgtttg tttcaagcta ttttttccta
     4681 agaaaagaaa aacagcaaaa aataattcta ataatacaaa aaataagata aagctatgca
     4741 tgaaaaattc ttctggaagt acattgatga ttggatcagt aacgggatcg aataaccagt
     4801 catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca
     4861 tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg aaacggacga atcaaccgcc
     4921 aaagccgatc attcttttgc aaatatttga taaacaaaat actcgggatc aaagtgatga
     4981 aaaacacagc ataatccact aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc
     5041 tagcagaaac tgggaaatct ggcaagctga ggatggtttt gaagggatta ttgagataat
     5101 tcattaggtg atcaaaattt ttcagtaacg tctcttgatc taaagaagta taatccaaaa
     5161 tttgcaaatg tccgatatca aatacgtata gtggtcgaaa gttgatcgtc acaccaactg
     5221 ccagactgat cagtgtcaga aataggctga acaatccagc atattccatc cactgccatc
     5281 tcgaattttt catattagaa gctccattca tccagtgatt gtttcacata ggtagctggg
     5341 ataggcaacc caggtacatc ttcttctttt gtaaaacctg ataaaacgag caaagtatct
     5401 atattattgc gtatcccagc ttggatatct gtttcatagt tatctccaac catgattact
     5461 tcttcttttt gcagccctaa tacttcaacg gctttatcca taatgatggc ttctggtttt
     5521 ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa aagagatgac tgaccctgct
     5581 ccaggaagaa ggccgcgttc agtaggtata ttcttatctg ggttcgttcc aatgaaagta
     5641 gcaccttttt gaatagcaag tgttgcttta actacttttt cataggtcag ataattatct
     5701 aagcctacca ctacatagtc aggagcctct tcttcccatg taaatccggc agctaaaatc
     5761 aagtcaatca atcctgcttc tccgataaca tagacttttt tcccttttcc atctgctttc
     5821 ataaaatcga ttgttgccaa agtagctgtg tagactgtct ctggagctac atgaatgtca
     5881 aactcatctg ctagacgatt agctactgtt tctggtgact tagtcgtgtt attggtaaca
     5941 aacaagaaag gtaattttct tttttgcaat tcttccacaa atcgtttacc ggccggtatg
     6001 ggttgtgtcc cacgataaat cgttccgtct aaatcaatta aatatccttt atagtccatt
     6061 catccactac tcttcccttt ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt
     6121 aggttgttct tgttttttta ctttttcaga ggtttttgct gactctgttt ttcgattttt
     6181 aataaccggt tttttgcgat tagatgcgac tggtcctttt ttctcatcga tatacgcacg
     6241 attatggttt tttttctttt ttcggcgctg agttgtcttt tcttttttac cgccgattct
     6301 ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg taaagatagt cttccaagga
     6361 atcgattcgc tgatctggat gcgcccgtcg attatccgct tggaaaaatc cttttaggcg
     6421 tagttgttca taaccccaat ctcctacgat ataatcatat cgggctagta catcactgta
     6481 acgttctcca agcttttctg cattaaatcc ttcacgataa tcggacacaa gccgataatg
     6541 tctttcacct atcaaaaaat tactgtcatc taacagtgtc actagctcgc ctttgacttt
     6601 ttctgcttta ggctcctcaa cgatttcttc caaagcagct gtcagttctt cggtgactgt
     6661 tgtttcctgt tcagctgatg attttttctt agcaatttct gttttatgct caacgttctg
     6721 ctgttcattt tgttttgcag acatgcgttc accacctatc ttttttatta taccttgttt
     6781 ttatttgatt gggtagtgag ctttcaagta atctcctacc acacttcgga taaattcagg
     6841 gaacaaaaat tcattttttc cagcaatctc aatcgttgga aaaaatggaa caaacatcaa
     6901 atgatctacg gtagtaaaag tataattttt attcagatcg attggcttgt tcttccaaaa
     6961 gacttgatgg ttaatagaat catatgtgat tccattatag accatctgcc caaaaatctt
     7021 accgcgaaat cccattccct tcataggata gttacgtaaa aagttacgat ttttttctat
     7081 ttctaaaact aagcggatca gatcatttcc acccaatgtg acatttaaca agtgcatcgg
     7141 atgcggcaac gcagtgtgaa gctgatcctg attgacccgc ccttttggaa tttcagttaa
     7201 aaacagacca ctgtttaata ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa
     7261 ggctacttgg ataaacgaat gctcattgaa gatatctaga ctcagagaaa atggcaaatc
     7321 tgctacttct ttttctttta ataattcatg cccacgttct agataactat ttatttcttg
     7381 ttcatcttct gtaaaagctg tcattgtttc ggtagggatg gctttggctg aatgcttaag
     7441 gatttttttc tcttcatcga tggttaaatg gacttcaccg acatactgcc cgtactttcc
     7501 agcggctgct atttggacat cattgattac gtgaccttct ctgaataaat gatgggtgtg
     7561 cgatcctaaa ataacatcaa ttgctggtat ttcttttgca atttggaaat catcctgaat
     7621 cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc
     7681 tggcaaaata tcataaggat tccgtatgtc ccagccattc ggattataag taagcggaaa
     7741 tggagcagta aatgcaatta gtccgatttt cgtctggtgt ttcgttgtaa tgattttata
     7801 tggtttcgtc catgccggtg gctggagcgt atttttatcg aataagttat ccaggatgat
     7861 atcaaactca gcatgatcgt ataaatgatt caactcttct ttggaatttc ctacgccttc
     7921 attgtttcct atcgtcgctg catcataacc aacttcattc atcagcaaga tattttcctg
     7981 tccattcgtt gcttctgaca atggatgcca acgatccaca aaatcaccta gatctactgc
     8041 aatcgttgtc gttttttctt tcttttcgtt ttctcgttta cgttgatcca aaaaccttct
     8101 gatccttggc caattttcta aatgagaatg cagatcattc gtatgtaaaa tcactatttt
     8161 ttccatgtac cctctcgcct ctctttatct ttatcatacc ataggcagct ttttcttcca
     8221 ttgaaacaag ctctatttca gagtttttcc tgccttgaag caaattgtat aagtacaata
     8281 ttcgataaat cagtctggct aaaaaatcag gaacattcat ttttacagta ctataaaaat
     8341 aaaaaaaaca gacccgaaag tctgttgtcg tttcattata atcagacaca tggcggaacc
     8401 tgcttagtgc tgcttccttc cagacctgac acgcttcaca agcccaccat tgtcctagcc
     8461 tttcggcaaa gaatagtata ccttattttt cattcgttga ccagtctttt tttaatttct
     8521 tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat catcccacat tcgtcttcta
     8581 aaatgccacc ttctacatat gcttggtggt taaaccgctc gtcctccaaa agattcatca
     8641 aggtaccggc tgttccgcct tttggatcat acgcgccgaa ataaacttct gctacacgag
     8701 acaaaatcat cgcgccgctg cacataggac aaggctctaa ggtaacaaaa agtgcagcat
     8761 cttccaagcg ccagcttccc actttttcac aggcttcctt gattgcaagc atttctgcat
     8821 gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc tcttccaatc acttttccat
     8881 ccaatacaac cactgctcca atcggaactt catgaagcat ttctgctttc tttgcttctg
     8941 ccaatgcaag gcgcatccat ttttcttttt ctactgcatc aagatttgta tttaccaatt
     9001 acccttccct gcctttgttt tttcttgttt tactttagca tatgataaaa aggctgtaac
     9061 taactttttt ttctatggta aaataaatac aagaggtgaa caagatgaaa atggataaat
     9121 tacttgctct ttacccacaa gcacagaaaa aaaactctcc agcatcagaa gcggatatcc
     9181 tttcttttgc aattgatgat gcttttatct ggatcaaaca agattcatta tctcaacaag
     9241 aaaccagcct tttgaaagcg ttattccctg taatcaacga tcaaaaaaaa cacccatggt
     9301 atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc
     9361 ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg gcaagatact attttggaat
     9421 tatttcgcta ttctgctgat ttcttttttt atacagacac agatgcgctc ttagtcgaac
     9481 aaataaaccg tgattatctt gatgctactg aaatcaacgg tatttttcta tctttagatg
     9541 ctgattttga tacgacaacg tctgtattcg ttggttcttt ccattcacct ggcaccgacc
     9601 tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac agagcaaaac aatctttata
     9661 ctcacagccg tactttttct atgcaagatg tggctttaca ttactataca aaagatactg
     9721 taaaagatag taagttgatc aaaagttaca tagaactgat caaacaaaat gaaatggaag
     9781 tgatcattca agaattatgg aatgaattag gcaatgtcag ctctgctgca aaaacactgt
     9841 ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca
     9901 atctaaaaaa agccaacgat ttactgtttt gctatctttt attgcttcat gaacataatt
     9961 aaagatagat taaaggggtt gtgacaataa ttttgtcaca accccttatt tcgaatgaat
    10021 agtgttcaaa agctagtttc tcttacatca ttttttcaac aaaatgcatt agccggtctt
    10081 ttacatcttc ggttgaatct ttgattgaat caccataaac agagattttc ttttttcctg
    10141 atttgatttt tgctaacatt cccatcaaag aatccaagtc actatctgaa agaccatcta
    10201 cgatttctag taatttttca tttccatcaa atttgtcata gacgaatttt tttactttcg
    10261 tccgagtagt catatgatgg atcttttcca ttacttttcc cgatacaacg actgctgtcg
    10321 ctacactggc agcggctgct acgcttaatc cgattgttac ttttgttgat gttttcataa
    10381 gtatcagctc ctttttatct atctccatca tagcatagac aaaaaaataa acgaactaat
    10441 ccactcagtg aaatctcaat cctttaggga aaaagttctt tactgttcct tttacttgtt
    10501 tcccaaatcc gacaggtatc ttatctaata ctagcaaaac ccatccttga ttcccatcac
    10561 gttgaaatgt ttctcctgct gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag
    10621 gtatattctc tatctttttt gtagcaagcg ctaatgcata gctaggttca aatcgattct
    10681 ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt taaaccatcg agagaagggg
    10741 ctaactcagg aacttcccaa agatggtcgt tgaacactaa aagtcttcca gtagcttcat
    10801 aatgaaaatc atttgaaaac tccgtccaga gtttttcctg ctcttttgtc atttgcactt
    10861 tacttttttt tcgtgtcttc ttttctttat gcatctgatt ttgtccatga aaagtcagct
    10921 ttgcaacaaa atgtccttca ccctgatctt tatgaggcca tatacgtatt gttttttcca
    10981 aaccagctac gcttccccat tcagaacgtc ctgaactaac ggattgagtc aaaggaatct
    11041 cctcaatcgt tactggataa ttttctacaa gccagctaat gatctcttca ttttcttctg
    11101 gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt ttttagcatt tttattgctg
    11161 aagacaaaat ctcttgctga cgtttttgac agtatagcgg agattcttct gtccactctt
    11221 ttattgcatt tggatctttt cggaacattc cttctcctga acaaggagca tcaaccacga
    11281 tccgatcaaa aaagcccgaa aaatgaggga caagttctgc gggtgcatga tttgtcacta
    11341 ttgcgttcga aactccccat cgttctatat tttctgataa aatcttcgca cgtttcggaa
    11401 agatctcatt cgtcactaat aaccctttcc ccttcatttg agcagctaat tgggttgatt
    11461 taccaccggg agccgcacac aaatccaaaa ctttttcacc gggttttgct gcagcagctg
    11521 ttccaacgat cattgcactt ggttcttggc tatattcata cccagcttga tggaggaaag
    11581 actttccatt gacagtgcca agaaatccct cattgctgta gggagcgggt tgtaattctt
    11641 cgctatggta agtttggacc atgtccaatc ctgccggttt tagtggattc caccgaaatc
    11701 cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa
    11761 gaagacgata ttttttgata aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa
    11821 tgtttcgtta gacattttac aatctttttt acgaaaaata aaggtggctg atctactttt
    11881 tcatgttgct gcctaaggtg actgttcctt taagatattt tccaagtatt catccgtcac
    11941 ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa tcttgagaag aatcatctga
    12001 gcgcaaactt cccaatgcat taagccatac caattcaagc gacttttctt ctttgatctt
    12061 cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc
    12121 agattttgca atgatttcac gaatactttc atggatagaa aaaaaatctt tcaataagta
    12181 gacaatgctt aaaacatcat cggaactgca ttcagctaag tagttttcaa aataaacagt
    12241 gatttcttct aatatcttct cttttaaccc gctgattgat ttgaaatgtg tataaatcgg
    12301 gtatgtagaa caaccgcaga actctgctaa ctctcttgca gtaattgact tcattccata
    12361 ttcttccacg tataccatac ttttgaacag caaatattct ttactataat ttgttactct
    12421 ccccatcatt cttacctccg tctcttttca tctacccata aataaataac ataaataatt
    12481 ctaaaaatat tattgataat tatcaaaaca tatttagtta tatccattaa aaatcaattt
    12541 ttccgcctta aaaacaagaa tatcttgaca aaatacccta ggaaataaac aatagaataa
    12601 acaaggtaac aaaaaaatta aataacgtta tataaaaaag aaggcgacac tctgtgagaa
    12661 taacacagac tgaaatgact ttccactcac agtttttgtc taactatata caaaaacaga
    12721 ataaaaagga ggtatttatg ctaattgaag caagaaaaaa agagttactt gttctacaaa
    12781 tggtcattca aaaaaataat tgttctttac aagaattatc caatgactta catattccaa
    12841 aacggacaat caaagaactg atccgaaaaa taaatgtatc actcaagcaa tttttattga
    12901 ttgatgaatt tatttattct aatcataaag gagagatcca aaccaaagaa accgatcaaa
    12961 ctaaaaagtt agctgttttt tctaagttaa aattatgcta cctaaaagaa tccaatcgtt
    13021 ttaactattt gatgttacta atcaattatc cacaaactgc tgtaccaaaa aaatatttac
    13081 ttgaacaact ttatatatcg ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt
    13141 ccttaaaaaa attccattta cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa
    13201 atgagttatc tatccgtctc tatctttact tttttctttc agatacattt ggaggtaacg
    13261 attggccatt ttcctcactt gaattaaatg cactaaaaat gaacagtaca gatgagcaaa
    13321 tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct gttaacttta ttcatattac
    13381 gaaacaaaca acaggcatat tttccaaaaa cttcttctga aatcactgaa ttgatggact
    13441 tattgcagca tacacatgat tattcagaat actttacttc tttttcagtg agtcgccctt
    13501 gtgaagagac agaaacagag agagcctact ttaattattt tttctattgt ttttttccaa
    13561 atcaaatctc ttttcgacaa aaacaggaga ttggtttttt attttccaca agcaaaaacc
    13621 ttatttgtcg acaagtcggt caattgatga cttacttaac tcaacagttt cctctcaacc
    13681 atttagaaaa taaaatacca atttattttt attttcttgt gttggcagtt gtttttttcg
    13741 aactgttggg tgaatccagc agtagttttt ctacactttg ctttccagaa gccacgtcca
    13801 atcattctag ttcttttata caggaaaaga aattaagaga aaaaatcgat acaatttttt
    13861 caaaaaatgg ggaaagaaac caaatcagtc aataccttag tcaattgatc caacacttac
    13921 tacaaacaga aacgaaagag cagttactca tccatttgca gataaccaaa cagtttacag
    13981 gaaattatca tcttcaaagc catttgcagc agttatttcg caaagatgct gttaaaatta
    14041 ccgcatattc agaacatgct gatttgatca ttacagatca gcatgagaaa atttcagatg
    14101 aaacggagac tttctatctg gatacaacgc acaatacagt gttatttggc aagttaataa
    14161 aagcggttca tgaactgtat atgaaaaaag gttatgagaa caaaacaaag aggatgtgac
    14221 aaaattcttg tcacatcctc ttattc
//
LOCUS       DARBER010000059        13986 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DARBER010000059 DARBER010000000
VERSION     DARBER010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.060"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            257..2728
                     /locus_tag="SCK06_001665"
     CDS             257..2728
                     /locus_tag="SCK06_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1151664.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2831..4567)
                     /locus_tag="SCK06_001666"
     CDS             complement(2831..4567)
                     /locus_tag="SCK06_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG1151665.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4564..5268)
                     /locus_tag="SCK06_001667"
     CDS             complement(4564..5268)
                     /locus_tag="SCK06_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1151666.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5399..5902
                     /locus_tag="SCK06_001668"
     CDS             5399..5902
                     /locus_tag="SCK06_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG1151667.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5862..6200
                     /locus_tag="SCK06_001669"
     CDS             5862..6200
                     /locus_tag="SCK06_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG1151668.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6221..7639
                     /gene="ffh"
                     /locus_tag="SCK06_001670"
     CDS             6221..7639
                     /gene="ffh"
                     /locus_tag="SCK06_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG1151669.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7751..8329
                     /locus_tag="SCK06_001671"
     CDS             7751..8329
                     /locus_tag="SCK06_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG1151670.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8333..8854
                     /locus_tag="SCK06_001672"
     CDS             8333..8854
                     /locus_tag="SCK06_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1151671.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8933..9487)
                     /locus_tag="SCK06_001673"
     CDS             complement(8933..9487)
                     /locus_tag="SCK06_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1151672.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9740..10015
                     /gene="rpsP"
                     /locus_tag="SCK06_001674"
     CDS             9740..10015
                     /gene="rpsP"
                     /locus_tag="SCK06_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG1151673.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10027..10278
                     /locus_tag="SCK06_001675"
     CDS             10027..10278
                     /locus_tag="SCK06_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG1151674.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10403..11194
                     /locus_tag="SCK06_001676"
     CDS             10403..11194
                     /locus_tag="SCK06_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151675.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11364..11885
                     /gene="rimM"
                     /locus_tag="SCK06_001677"
     CDS             11364..11885
                     /gene="rimM"
                     /locus_tag="SCK06_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG1151676.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11875..12636
                     /gene="trmD"
                     /locus_tag="SCK06_001678"
     CDS             11875..12636
                     /gene="trmD"
                     /locus_tag="SCK06_001678"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG1151677.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12772..13119
                     /gene="rplS"
                     /locus_tag="SCK06_001679"
     CDS             12772..13119
                     /gene="rplS"
                     /locus_tag="SCK06_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG1151678.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13251..>13986)
                     /locus_tag="SCK06_001680"
     CDS             complement(13251..>13986)
                     /locus_tag="SCK06_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1151679.1"
                     /translation="KYVSVIKSKLNLLFSYAMKKGYVEKNPINEVVIDYKRESKTIKI
                     KDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASV
                     VINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTT
                     KGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHEN
                     SDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         4587 a         2542 c         2851 g         4006 t
ORIGIN      
        1 taggatgacg ttttggtcga ctaaatttta ccaaaagtga gtctcttttt gagttgaaac
       61 gaagaaaaag tgttagtaga aaatcgcctt cgattttcca ccaacacaaa atattataga
      121 accctttttt tttgctatat gacaggaatg aaaggtgttt gcaagcttaa tgtgaggaac
      181 actttccata tctgttctat actggatagg agaagtttgg aaatatccag aaaacgaaat
      241 caatcgaggt agccacatga agaataaatg gataaatcga agtaaaaaaa agaaagtaac
      301 acaaagatca tcagataaag tcatccgtct ttttccatcg tataaagaag gattaacggc
      361 agatcaagtc aaggaacgaa tagaaaaagg tgcagcaaat aattcagtcg atcccacatt
      421 caaaacaaat cagcaaatcg tattggaaaa catatttacg tatttcaatt tgatctttct
      481 tatcttagcc attttgcttt gtttagtaga atcctataag aacttgactt ttttaccagt
      541 tatcatagcc aatacaggaa tcgccatttt tcaggaaatt cgatccaaaa agatcttgga
      601 cgaactaaat gttttaaata cagcaaaagt caatgtcata cgagacggga tagaacaaac
      661 aatagatata gaagaccttg taatagatga cattgttatt ttagagactg gatatcaaat
      721 tccagcagat gcagaggtcg tggaagggaa actgcaagtt aatgaggctt tattgacagg
      781 cgaagcagat gaaataacca aagaaatagg tgatccatta atgtccggca gtttcattgt
      841 ttccggaaaa gcttacgccc gattagacaa agttggagca gattcgtata tttcccagct
      901 gacagtcaaa gccaagacaa tgggagaagg agaacagtcg gagatgattg cttctcttaa
      961 tcaattgatc aaatggattg ggatcatcat tattcctatc ggtatcctgc ttttttctca
     1021 aagttacttt tttaatggaa acacgatcaa agaaagtgtc gttgcaatgg aagcagcatt
     1081 gatcggtatg atacctgaag gactgtactt gctgacgacg attgcattag cattaagtgc
     1141 gactaaacta gcaaagcaaa gagttctttt acacaatatg aaaagcattg agacattggc
     1201 aagggtaaat gtcctttgtg tagataaaac aggaacgatc acagaaaata aaatgtctgt
     1261 tcaaaaaatg atcgtctcta aaaaacaaga aataatcagt cagtcagatc aattggaagc
     1321 atgcattgct gactatgcaa aagcgatggc aggagacaat gcaacaatgg aagctgttaa
     1381 agcatatttt acaaaaacca ctgatcaatc ctatcaccat gttattcctt tttcttctgt
     1441 ccaaaaattc agcagtgtag cgttggcaga caaggtgtat gttttaggag cgcccgaaat
     1501 ggttttgcgc gatcaattgc cggaatatgc ggaagagttt gtttctttcg ctgaaaaagg
     1561 ctatcgcgtg ttggtctttg gtgtgtacga tgggattctt gaagaaccag tattgacaga
     1621 agcagtcaca ccattaggct atattctgat tgctaatcct atcagaaaag aagcaagagc
     1681 cacatttgat tacttcaaaa aacagcaagt agccatcaaa gtgatttctg gagataatcc
     1741 gctcaccgta tccaatgtag ctgtccaagc agggatttca catgctgatc agtacgttga
     1801 tgcatctaaa ttatcagaag aagaatatga agcagctgtc gaaaaatata cagtttttgg
     1861 acgcgtcaag ccagaacaaa agaaaatctt cgttcaattg ataaagcgta acaatacagt
     1921 agctatgaca ggtgatggcg tgaatgatat tctagccatg aaagaagcag actgcagtat
     1981 agcgatggct tccggaaatg aagccgccat gcaggcctca caagtggtct tacttgattc
     2041 tgacttttca cgcatgccag aagttgttgc agaaggacgg cgcgttgtga acaatattga
     2101 gcgatcagcc agcttgtttt tagtgaagaa tattttctcc tttttgctct cactgttttc
     2161 agtgatcttt gctctgacct atcctttaga accatcacaa atcacattga ttagtttgtt
     2221 tactatcgga cttccatctt tcttgcttgc tctagaagaa aacaaaaagc gaatcagagg
     2281 gaaattcatc atgaatgtga tggaaaaagc tgtgccaggt ggactaacag atatgatcgt
     2341 agtcggtgcg ttggtgatct gcggggtgac tttgaatcta aataaaacag atgtttctac
     2401 agcatctacc atgcttttga ttgcagtagg gttcctcgtt ctctataaaa tctgttcacc
     2461 gctgaataaa ttcagaagtc agatcattct tttctgtgct agcggtaact ttttttcggt
     2521 catctttttg cataagctgt tttcaatcac cggtatctca gctgtatcac ttttgctgtt
     2581 gagcattcta tttttctcgg cagattcgat ttttaggcat ttgaccacat tggtagaata
     2641 tttgtttgaa cgtaaaaatg aaaaagagca aaagccgttt agtcgcgtag ctaaaaaatt
     2701 tctatcgata tttaaacgag aaaattgacg aaaagtatct caaaaaagag taaaaaagaa
     2761 caaccactcg tgtatctttc gaagcaggat gagtcatcca actttaaaac gcacgagcag
     2821 ttgctctttt ttacattttc ggaatggtaa cggtgaaagt cgaacctgtt ccaagatgac
     2881 tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc ttttacaatc gctaacccta
     2941 atccggtccc gcctgaatgt cggcttcgcg ccttgtctac gcggtaaaaa cgctcaaaga
     3001 tccgttcctg atcctcaatg tcgataccta taccaaagtc ttggacagaa aacgatagat
     3061 catctgttgc ctgatagcgg ataataatct cgctgtcgct ttttgaatat tggacagcat
     3121 tttcaattag atttttgata attggataaa ggatatctgt tttagtagta aatggttcgt
     3181 tctttccaat caatcgaatc gtcaaccgtt tcttttcgat gattggctga taattttgaa
     3241 cgatttgctg gaaataaggt tctgtataaa ttgtctggat ctcgtaagaa ttctcgccat
     3301 cttttgacag ctggatgatt tcccgaatca gtttatctaa ccggatcgca tctttttgca
     3361 tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt tgcaccatcc agtaaagtct
     3421 cggtaaaacc aatcagtgaa gtcacaggag tttttaattc gtgagagacg ttgccaacga
     3481 aatccttttg tagcttttct aaacgtcgca ctttcgttaa gtcatacgcc acccctagaa
     3541 tctgtccggt accttctgaa ttagtaaagt agcgcaaact caagtctaat gtctgccgac
     3601 cttctgtaag ggtgatctcc tgatggatca gcggatgctc aggagtcact tgatgaatca
     3661 gctgaatgag ttttggctct tgaatgactt ctgtgtaccg ctgttcaggt tgatagctta
     3721 ccacgtttaa atgtagctgc atctttggat taagcagtag taaacgagaa tcagcatcat
     3781 caatcaggaa tacgccaatc atcagttcgt tcagtaacgt atataattgc tcttctgtgg
     3841 tagtacacgc ccgatacatc ttactcattt gttcgctcaa cgcattaatc gtctgataca
     3901 attcttccca ttgatcgggt gtttgcatga ttacttccgc ctgattagga ttttgcacca
     3961 ttttttttag tacgggcagc acggttttca atggctggtt tttttgatag atcagataat
     4021 agatcattcc tgcaagaaca aggaagaaag acagaaagaa gaaaaagacc caacggcgaa
     4081 aactctcagt tctcggtaaa aaaccgcttg tcggttctgc gatacgtaaa atggcttcta
     4141 actggccact ttttttgact ggtaatgcga catataaaag atcctcattc aaagtagtac
     4201 ttttgcgcaa agcgctacct aggttcccac cattcaatac ggctttgatt tcgggacggg
     4261 agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa taagatcttt ccagtagcat
     4321 ccattaaagt aatccgttcg ttcgactgat ggacgaattc ttccaaagca gttttgtttt
     4381 gcgctgcttg gaggttttcg acatctaatt gccggatcag cagttgtgct ttttttgtca
     4441 ggtagctttc ttgctgttcc acgatctgct gttgaaaata atgggaaatc aattgccagc
     4501 caccaaaaaa caaactaact aaaagaataa gaaagaagag acgttcataa atttttttcc
     4561 atttcatcgt ttgggttcct gaaacttata gccaaatcct ctaatagtca ccaagtactt
     4621 tgggtgtttt ggatctcgct cgattttttc tcgcaaatga ctaacgtgta catctacgat
     4681 tcggctttgt cctgcgaagt caaaattcca aatgcggtct agcaatgtgt ctcggtcgat
     4741 cacacggtct tttcgtttca tgaaataaac aagtaattca aattctttcg gtgttagttc
     4801 aatcagatgg tcgtctacgg tcacttgata attggtcaaa tcagcagtga tttgtccaat
     4861 cgatagatat tcaggctcag cttcatcttt tgattgctta cgaggttcaa ttcgtctgaa
     4921 tattgctttc atacgggcta atacttcgcg aggactaaat ggtttggtca aatagtcgtc
     4981 agcacctatt tctaggccaa tgatccgatc cacttggtca tctttagccg tcagcatcaa
     5041 aatcggtgtt tcattttttt cttgacgcag ccgctgagtg atctccatgc catccatcgt
     5101 tggaagcatc acatctaaaa tgatgaaatc aaatgattgt tctaatgcta attctaatcc
     5161 ttcgcctcca tctgtggcac tggttacctt gtagccttct ttttccaaat tgaacgtcaa
     5221 gagcgttaca atagaaggtt catcatcaac aactagtatt tttttcattc aaatctcctt
     5281 taaataggtt tcattctgtt tatattttag catagaatca gtacaaaggg ctattttaca
     5341 aaagaaataa atggtataat aggaaggcac tttgctgaat agaaaggaag agcagtcaat
     5401 gattggaatc agtgcatgtt taggcggtct ggcctgtcgc tatgatggac agaaaaaaga
     5461 agtaccagaa ttgatggaac tagttgaaac tggagaagct gtcatggtct gtccagaagt
     5521 gatcggcgga ttaccaattc ctagagatcc cgctgaaatc attggtggaa atggattcga
     5581 tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc gaagatgtaa cggaagcgta
     5641 caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa aaacacattg atctagtcat
     5701 catgaaggaa aaaagccctt catgcggaac ccattcgatt tatgacggaa gtttttccgg
     5761 aataaaaaaa aatggtgccg gagtggctac tgcttatttt attcagcaaa aaatgactgt
     5821 gctctctgat gaagaatggc tgaaaactag aggtgagcag aatggaaatc gaaaagacca
     5881 atcgaatgaa cgcgctcttt gaattttatt cgaccttatt gacagaaaaa caaatgaact
     5941 acatggaatt atattatgca gatgattttt cattaggtga gatcgcggaa gaatacgaag
     6001 taagccgaca agcagtatat gacaatatca agcggacaag caagatcttg gaggattatg
     6061 aaaaaaaact ccatcttttt tctaattata ttgttcgaga acaggtattg gaaaatatga
     6121 ctgcatatat tacagataag tacccggaag ataaaaaact acttgaatat gtacagcaaa
     6181 ttcaagcaat cgaagaataa ataaaaggaa tgaagaaaat atggcatttg aaagcttaac
     6241 tgaacgtctc cagcaggcga tgggaaaaat aagaaaaaaa ggaaaagtat cagaagctga
     6301 cgtaaaagaa atgatgcggg aaatccgcct tgccttacta gaagccgatg ttaacttgca
     6361 agtagtcaaa gattttacta agcgcgtacg cgaacgtgcg gtaggggcag aagtgctgga
     6421 aagtttatca cctgcacaac aaatcgtaaa aatcgttgat gaagaattga cgatcacact
     6481 tggttctgaa acagcagagt taaataaatc accaaaaata ccaactgtta tcatgatggc
     6541 tggtcttcaa ggggcaggta aaacgacctt tactggtaaa ttagccaatt atttaaagaa
     6601 aaatgaaaat gctcgtccgc tcctaatcgc aggggacgta tatcgaccag ctgcgatcga
     6661 ccaattgaaa gtattaggtc aacagctaga cgttcctgta tttgatatgg gaactgaagt
     6721 cagcccggta gagatcgtcc gtcaagggat ggagttagca aaagaaaaga aaaacgacta
     6781 tgtattgatc gatacggcag gacgtctgca tatcgatgaa acattgatgg atgagttgaa
     6841 acaaatcaaa gaattgacgc aaccaaatga gattttgctt gttgtcgatg ccatgactgg
     6901 tcaagatgcc gtcaacgtag ctgatagctt caaccagcaa ttaggtatta caggggtcgt
     6961 catcactaaa cttgatgggg atactcgagg cggggcagca ctatcgatcc gctcagtgac
     7021 aggcgcacca atcaaattca tcggttctgg tgaaaaattg actgacttag aagtattcca
     7081 cccagatcgt atggctagcc gtatccttgg tatgggggat atgctgacat tgattgaaaa
     7141 agcgcagcaa gactacgacg agaaaaaagc ggaagaactt gcacagaaaa tgcgggaaaa
     7201 ttcattcgat ttcaatgact ttattgaaca gcttgatcaa gtgatgggta tgggcccact
     7261 ggaagatcta atcaaaatga tcccaggaat gaatcaagtc cctggaatcg aaaacgtcaa
     7321 ggtagatcca aaagatgttg aacgcaaaaa agcgatggtt tattctatga cgccagcaga
     7381 aagagcaaat cctgatctgt tgaacccaag ccgtcgtcgc cgaattgctg ccggttctgg
     7441 aaacagtgtt gttgaagtga atcgaatgat caaacaattc aaggaatcaa gaaaaatgat
     7501 gcagcaaatg tccaaagggg atatgaacat cccaggtatg gaccaaatgt ttggttcagg
     7561 cgtcaaagga aaactaggca aaatggcgat gaatcgaatg atcaagaaaa ataagaagaa
     7621 aaagaaaaaa cggaaataag atgaaaaaaa cagcttcggt acagactgaa aatgtctact
     7681 gccggagctt ttctatcagc aatttttatt ctttactaat acaattactc taaacgaaga
     7741 ggtggcacag atgagatgtc cgtggagtga acaaacagaa agtatgcggc aatatcatga
     7801 tactgtctgg ggtgttcctg aatatgatga ccaaaagctg ttccgaaaat tgatgcttga
     7861 tattaatcaa gccggattga gctggcagac gattttaaat aaaagtgaag cattcgatga
     7921 agcatatgat cgattcgata tcaaaaaagt cgcaaattat ggagaaacaa aaatcagtga
     7981 actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa atcgaagcag ccgtccaaaa
     8041 cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt ttttcagagt atctttggtc
     8101 gttttctaat ggaaaaatcg ttgattcttc ttatcattca caagaagaag tcccaacaac
     8161 aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa agaggattta aatttatcgg
     8221 aagcacaaca atctatgctt ttttagaagc tgtcgggatc atcaatgatc accttgacag
     8281 ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag ggcgaataaa gcatgcgaaa
     8341 aatcatattt tatggtgcta taagtttaga tggctatttg gcaacgaaaa cggatgatct
     8401 tcaatggctt tttgacactc caacaggtaa gcaaactact tatgaagctt tttatgaaac
     8461 aatcgatacg accatcatgg gcagaaaaac ttatcaggaa gcaaaaaaat atttggagac
     8521 cgaagcaatt tatccagaga aaaagaattt cgttttttcc acccacgcac aattacaatt
     8581 gccagatgct gaggtcgtaa acgaagatcc tgttgctttt ctaaaaaatc tacaaatgac
     8641 agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg atcaaaccgc tattggaaca
     8701 tcaaatgatt gatgaatggt atatccagat tacccctgta ttattagggg acgggattcc
     8761 tttattccaa aaaggaacat atgaagcgcg ttttgaaatg ctagacacga cacgattcgg
     8821 tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa ataaaataaa aaaggcaact
     8881 atatgcagaa atgtcctgta catagttgcc ttttttatta ccgttatgct ttttactctg
     8941 ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt ttggaagaaa cttaatgttc
     9001 cttcaacgat attaccattt tcatcaatta gattcgcaac gtttccgata tatgcttccg
     9061 gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg gcgtagatgg tggtttgcgc
     9121 caaagccgcc gatcccacct ggtgatgcag taacgactaa acctggttta tgatcccaaa
     9181 cactgtgtcc ataaggtctt gagcctacat ctaaagcatt tttcaatact gctggcacag
     9241 aacggttgta ttcaggagta acgaaaacga caccgtctag tcccctcact tcctcacgaa
     9301 aacgagtcca ttcagcagga gcctgttctg gagtttcgag atcctcattg taaaatggca
     9361 gatcatcgat ttttacaaat acactttcgt acccttcagg aagcaatttc gcaaaagctt
     9421 ctgctacttt tttgttgtaa gaatcttttc ttaaactacc aataaaaaat ccaatttttt
     9481 ttgtcatacg atttttcctc cttcaaacta tctataataa tcttacaaaa gataagtaac
     9541 tggagcaaat aaaaacactt actgacaaaa gctacataga agatagatta cttattttta
     9601 acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa tcgaatttaa tcaagctgta
     9661 aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac tacatcttgt gaataaaaca
     9721 atggaggtgg aaataaataa tggcagttaa aatccgttta aaacgtatgg gttctaaaaa
     9781 gagtccgttt taccgtatcg tcgtagctga ttcacgttct cctcgtgatg gacgtttcat
     9841 cgaaactgta ggtacttaca atcctttgaa agaccctgca gaagtagttt taaaagaaga
     9901 cttagttctt gactggttat caaaaggtgc acaaccttct gacactgtac gtaacatcct
     9961 ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa ttctcaaaga aataaggtga
    10021 ccggatatga aagatttaag cgacttagtc ttaacaatcg ttcgtccgct agtcacttat
    10081 cctgatcaag tcaagttaga cgtcgtagag tcgaaagact tttacgagta caatctaaca
    10141 gtggctcctg aagatattgg ccgtatcatt ggaaaacaag ggcgtgttgc taaagcaatc
    10201 cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa aagtgcgttt gaatatcatc
    10261 gatgataagg aaaagtaaat aaacgtgctg tactcgaatt ttcgagtgca gcttttttgt
    10321 tttttttatt aattgaagaa atgattattt ggcaaaacaa gcaagaaagc gtatccttat
    10381 aggaggaggg agtgaacagc caatgagaaa gaaaatgtta tttattccat ttgctctatt
    10441 gttaagcgct tgtagttcgg ttactcaaga atcagacaat acacatctat caagcagtat
    10501 atctgaaact gttaccagtc cagaaaaaag tagtaccaaa acggcttcca ccactgaaca
    10561 aacaatggct acctcaaata atgagaaaaa gactgcttta gaccagttga aagaacaaca
    10621 gccaaatgtt cccatgcctc tagatgtacc agtctcttca ggctatctca atatcgcagc
    10681 gacacataca aagcaaggtt attctatttt atattacaga acagatcgtc ccctcgggtt
    10741 gaatgctgac gaattgaatc aagaaacgcc tattgcaacc tatctatatc aatatgggtt
    10801 tgcttctagt caagaaacaa tccaagtttt gcagccattt gagatcgaca caaatggtca
    10861 gcaagtggac ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag gttcaagttt
    10921 tcttgaatgg caagaaggaa actggcgtat tcgaatccgt gggaataata tagaaggcca
    10981 agatccctta ctattagcta aagaaatcgt agcttatttg gaagaaaaca gtctaccagc
    11041 acctgaacaa tttggtaaaa ttactgttga tatgggtgat acgacgaatc gagccgtaga
    11101 agtaagctgg caagaaccga aaaatgccta tacgatcact catcaagatc ctatgtctgc
    11161 gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc tgagcccatc
    11221 atttaactcc aagtcacagg aagtaatcaa aaatagcacg ccgcattttt gcataatttt
    11281 ggcttaatac cttaaaagaa aactagaatt gcctcttcac acatgctaaa ataaaacaga
    11341 caaaaaagaa atggggatat aagttgactg aatacttgaa tgtaggaaaa atcgtaaaca
    11401 cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac ggatttccca gaagaacgtt
    11461 acaaaaaagg aacggtgcta acactattcc aagacggaaa agcacctgtg gaattaacag
    11521 tcaaaagtca tcgcaaacat aaaaattttg acttattgag ttttgaagga catccttcta
    11581 tcaatgatgt ggaaaaatat cgggatggca tattaaaagt gtcaaaagac aactctgtag
    11641 atctagctga aaatgaattc tattaccatg agatcatcgg tctgcatgta ttagaagatg
    11701 gaactgagtt aggtaaagta aaagaaatct tgtcaccagg tgccaatgat gtttgggtcg
    11761 ttcaacgtcc gaaaaaagct gatatattgc ttccatatat cgcttctgtc gtcaaagaag
    11821 tcgatttaga agctggagtg gtccgtgtag aaattccaga aggactgatt gacgatgaag
    11881 attgatgtat tgactttatt tccaagaatg tttgaaggtc ctatggggga atccatcatc
    11941 ggtaaggctg tagataaagg attattagat atcaatatct ctaacttccg cgattattca
    12001 gataacaaac atcagacagt agatgattat ccatacggcg gtggagcagg tatgctgctc
    12061 aaagtccagc ccatctatga taacatccaa gccattgaaa aagctgcacc ggatgtcaaa
    12121 aaaagagtaa ttcttcttga cccagctggt aaacgattcg atcagaaaat ggcagaagaa
    12181 ttttcacaag aagaacactt agtattcatt tgtggtcatt atgagggata tgatgaacgt
    12241 atccgcacat tagtgacaga tgaagtttcc ttaggagatt atgtccttac tgggggagaa
    12301 ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt tgccagatgt gttagggaat
    12361 cagacttcag ctcaaacgga ttcttattca accggtttat tggaacatcc ccaatatact
    12421 cgcccggcag aatataaagg gatgaaagta ccagaagtat tgacaaatgg aaatcataaa
    12481 ttgattgaag aatggcaatt aaaagaatca ctaagaagga cctatcaacg tcggcctgat
    12541 cttttggaaa cacttgaatg gacacctcaa atgacgaaat ttttagaaga aatcaagaaa
    12601 gaagaacaag aaaaaacagc agatgaagcc aagtgacttt ttctaaaatc ttctttacag
    12661 taggttgata atatgttagt attttatggt gagtgcaaaa gcacttaact attacgatat
    12721 tccgctgtga gaaaagctca taagaatatt tggaataagg agaagaaaac catgaatcca
    12781 ttaatcgaag aattaacaaa agaacaatta cgttctgaca tcccagcgtt ccgccctggt
    12841 gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat ccagttattt
    12901 gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac agtacgtaaa
    12961 gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc atacaccacg tgttgcaaaa
    13021 atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct acgtgcatta
    13081 cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat catcgttatc agactgagaa
    13141 agaacccttg gtattcaagg gttctttttt tgttctctgt ttttttatat taacgatttc
    13201 tatttgggac atttttgatt aataaaaagg ggcaaaaaag gggcaacaag ttacagtttt
    13261 tctatatctt ctttcaattt ttcttttact ccctttgtca catgcaaata aatcttttca
    13321 gtgatatcac tattttcgtg cccaacgcga tcctgaatag catacagagg agttcctagt
    13381 tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg aactaagttt tttatcaatc
    13441 ttcatgtcag ctttatgatt tctcaaatag gtgttaattg ctgttagttg gaaaggagtt
    13501 cctttcgttg tttgaaatag aaattgacca ttcggattta attctagaag ctcattgtaa
    13561 atagctatcg ctttttttgg taaatcaatt tctcgcattc cagcagcagt ttttgtagaa
    13621 tcagattttt tcatatcagc tattgaacgt tctctataca tcatcgtccc atttataact
    13681 actgatgcat tattattggt aatgtgtaca tcgtctttac ttaatgcgat tgcttctcca
    13741 ggcctcattc cagtcaaata tagccactgg aaaaggagag aatatctttt attgtgtgaa
    13801 gttgtgaaat ttactagtct tttatattca tcatcttcta aaaacttatc tttaattttt
    13861 atcgtttttg actctcgttt ataatcaata actacttcgt tgataggatt tttttctaca
    13921 tagccttttt tcatagcgta tgaaaaaaga agattcaatt tggatttaat tacactgacg
    13981 tactta
//
LOCUS       DARBER010000060        13900 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARBER010000060 DARBER010000000
VERSION     DARBER010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.061"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..179)
                     /locus_tag="SCK06_001681"
     CDS             complement(<1..179)
                     /locus_tag="SCK06_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1151680.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLAS"
     gene            complement(658..1377)
                     /locus_tag="SCK06_001682"
     CDS             complement(658..1377)
                     /locus_tag="SCK06_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1151681.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1426..3072)
                     /locus_tag="SCK06_001683"
     CDS             complement(1426..3072)
                     /locus_tag="SCK06_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1151682.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3251..3841
                     /locus_tag="SCK06_001684"
     CDS             3251..3841
                     /locus_tag="SCK06_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1151683.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3933..5438
                     /locus_tag="SCK06_001685"
     CDS             3933..5438
                     /locus_tag="SCK06_001685"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG1151684.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5522..6874)
                     /gene="celB"
                     /locus_tag="SCK06_001686"
     CDS             complement(5522..6874)
                     /gene="celB"
                     /locus_tag="SCK06_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1151685.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6874..7392)
                     /locus_tag="SCK06_001687"
     CDS             complement(6874..7392)
                     /locus_tag="SCK06_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151686.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7408..7734)
                     /locus_tag="SCK06_001688"
     CDS             complement(7408..7734)
                     /locus_tag="SCK06_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG1151687.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7747..9726)
                     /locus_tag="SCK06_001689"
     CDS             complement(7747..9726)
                     /locus_tag="SCK06_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1151688.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9747..10061)
                     /locus_tag="SCK06_001690"
     CDS             complement(9747..10061)
                     /locus_tag="SCK06_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG1151689.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10229..10522
                     /locus_tag="SCK06_001691"
     CDS             10229..10522
                     /locus_tag="SCK06_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG1151690.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10600..11712)
                     /locus_tag="SCK06_001692"
     CDS             complement(10600..11712)
                     /locus_tag="SCK06_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1151691.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12099..12278
                     /locus_tag="SCK06_001693"
     CDS             12099..12278
                     /locus_tag="SCK06_001693"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151692.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12472..12642
                     /locus_tag="SCK06_001694"
     CDS             12472..12642
                     /locus_tag="SCK06_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1151693.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13066..>13900)
                     /locus_tag="SCK06_001695"
     CDS             complement(13066..>13900)
                     /locus_tag="SCK06_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1151694.1"
                     /translation="QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVV
                     LTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPT
                     GYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDAN
                     GTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFL
                     TKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRN
                     I"
BASE COUNT         4337 a         2838 c         2381 g         4344 t
ORIGIN      
        1 aagctagcca agaacccatc tgctgaaata tgaaaatttt tgaatgctac gacattcgcc
       61 attacaggta tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc catcaggaca
      121 agtgctttat aacgccatag attcataaaa aaatgattga acccctttcg ttttttcatc
      181 taaaaacccc tttcgattca ttcgcaacgt aattatagcg ctttcaataa tgagaataaa
      241 taaacaaagt atagggaaaa tgaaacaaag tataggagag gttgtgaaat cagcgttttg
      301 gcctgagtat tagagaaaaa aacgggaaaa tagttcctca tattttgccg ttttttgagc
      361 taataccgag ggtcagctga ttgaacaccg tttatcagag gttgtgaatt cagcgttttg
      421 gcctgagtat tagggagatc gtgtccttac tatctaactc tgagtcacaa ggaacaatgg
      481 ggactgtaac aaaagtcttg tcacagtccc ttattccgaa caaacggtgt tccagaagta
      541 gcttctacgg aaataagccg aaatttcaca aaaaagccat ccgaaagtca gacggacaag
      601 tctgcttttc agatggcgct acaactactc tgcttattct tttagctatc cattcaatta
      661 tttttctctc acttgttcta gctcttcttt ggtcatgggt cgatattgcc ctttattcaa
      721 atctttaggc agccaaaatt ttcccatacg gattcttttt aaatataaga cttcttttcc
      781 tactgctttg accattcgct taacttggtg aaactttcct tcatgcaaaa tcagacggat
      841 aattgaagtt tctgtctctt gatctacttg atcaatttgg agttttgccg gcaatgtctg
      901 ctcatcgcca tcaataatca gtccttctgc aaataaacgg atatcttctt ctgtcattat
      961 accgctaacc tcagctagat attctttctc cacatgtttt ttaggtgata gtaactgatg
     1021 ggcaaacacc ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat ctaatcggcc
     1081 gactggaaaa agatcttccc gataatcctg gtcttccaat aaatcgatta cggtttgatc
     1141 atgattatcc tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt agtaaaaatc
     1201 tttttgatac gtaatgattt ctcctaaata agtaaccgca tcttcgaatt catttacttg
     1261 cgctttatcg cttttcacca cttgtccatt gacttcgatc gttccttttt tgattaatcc
     1321 tttgacttct ttgcggctgc caagtcccat atctgctaaa aacttatcta aacgcatgcg
     1381 tttcccccat ttcaaaagag catcaatcta gctcttctga ttcacttatt ttatttttaa
     1441 tttatgtcgt aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc ggattttcaa
     1501 agctaaataa ccatacacta aagcccctat acctgctact acaagaatca acaatagaga
     1561 ctggaatttg ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga tccacgaagc
     1621 caaaagcata atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga caaaacttgg
     1681 tttaaagcgg gctacttcat ggattctctt taagatcaag gtacaagaca cagcaaatcc
     1741 gatcattgtc gccaataaag ggccgtatac ttcaaataaa cgaatggctg ggtattgaag
     1801 aaggagtttc actagaaacc caataaccaa ataattaatt gccgccttgt tttcaaacat
     1861 tccttgcagc atgttggaaa ctagcatata tagtcctagg aagagtccta caaaacttgc
     1921 ttggatcagt acattacttc ctagagaatc cggtgtataa aataatgtat ttagcggata
     1981 ggcaagtaac ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca actgtaaatt
     2041 tccgcttgtc aattttgcca gacctactcg atctttcaac gtaacagctt ctgtaatcaa
     2101 cggtaaccca gtagacgcga tcgatgttgc caatgcaata actaccatcg tcaacttatc
     2161 tggattggca ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat actcggtgga
     2221 tgcagacatg atcttcataa aagtgaactg atcgactaat ttgaagatcg ttacccctga
     2281 acccacaata ataaacggaa tcgcttcttt aacggtatct aacaacaatt ccttcgttgc
     2341 aatatttatt ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt gatgtttctt
     2401 caaaaaatac aaaaggacaa gaatacttgc tagcataccg ataaatgccg cgaaagttga
     2461 ttgcgtgacc gccgtcacat agtcaccatc catgactttc atgatgatga atgtagccaa
     2521 tagcatataa aaaatacgtg ccacctgttc tacaatctgc gacagcgcat aaggcatcat
     2581 ttcttgattg ccttgcaaat accctcgtat cacactcata caaggaaaaa ccaatacagc
     2641 gatactcaaa gaacgcatcg ttggaatcag ttcttctccg ccaccagaag catgagctag
     2701 ccaaggagag gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc ccatcatctg
     2761 taatgcacgg ataaataatc gtcgtgaagt gccatattca ttcaatgaat tatagcgtgc
     2821 agtttgtttt gcaatcgcag caggtattcc tgctgtagag atcagtagaa acaatgcata
     2881 tatattgtat cccatattga ataatccatt ggcagcctta gcattttcgc ccatccaagc
     2941 ataccaggga atgatataga cagcgcctag caatcttgaa atgatattac tggcggtcat
     3001 ccaagcagag cctcgtgcca ttttttcttg cggtgttaat tgatctaatg ctggttttct
     3061 ttgattatcc atcggtcaga ttccaacttt ctacaaaaca ttactatcat tttaattggt
     3121 aaaacagaaa ctggcaatcg attttatata acttttataa gaacaattgt ttttttgaat
     3181 tcggtttcgt ttttttggta aagtaaagaa atatacagcc ttttatggct aacatttata
     3241 ggagatatca atgacacaag aaataaaaaa actacccgtt atatttttag gtcttttctt
     3301 tatcacagtt ggattgaatt ggttcttgct ccctcacgat atagcttccg ccggagtggg
     3361 cgccatcgga catttagttg aaacggcctt ttctatccaa cgctcagtta ctgtttggag
     3421 catcaatctt atgatgctgc ttctagccgc tgtcctatta ggaaaatcag tattttttaa
     3481 gactgtagcg ggaagtctgc tttttcctgt tgttttagag attgttccaa aagtggaaat
     3541 gatctcctca catttctttt cactgatttc aggaagtctt ttgttcagtc ttggggttta
     3601 cacattgtac acagtcgggg cctcaaatgg cggtataacg ataccgccga ttattttcca
     3661 caaatttttt cggcttccaa tgccgcaagg acttttactt accaatagtc ttatcgtctt
     3721 tttgaattat tatgtttttg gattgttgga gacactattc gttctcctat ctattacagt
     3781 cagttcattg tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa aatttgaata
     3841 aagctggatt ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg ctataataaa
     3901 ccaagaaatt gaataaagcg agggatcaat caatgagctt ctctttagaa gacatccgtc
     3961 atttactatt aaaagaaaat ctattaaaag aatttgtttc aacaagaggc tggcatcttg
     4021 atgtaccaga aaaagctgaa tttcttcagt taagttatga ttcaagaaaa gctgatgctt
     4081 ctactttgtt tttctgtaaa ggaatgaatt tcaaagaaga atatttggat tcagcaatcg
     4141 aacagggtat tcaatattat gtatcagaac aaccatacga aaattcagca gtaggaatca
     4201 tcgtaacgga tatccgtaaa gcaatggcgc ttcttgctat ggctttttac gactatccgc
     4261 aaaataaatt gaaagtgatc ggattcactg gaacaaaagg taaaacaact gctgcgtact
     4321 ttacaaaagc aatcttggat cacacaacaa ataaaaaaac agcattgctt tctactatga
     4381 atacaacgtt agatggcaaa acatatttca aatcccattt gacaacgcct gagtcattag
     4441 atttatatcg tatgatgtca gaagcagttg aaaatggtat gactcatcta gtgatggaag
     4501 tttcttcaca agcttacaaa acacaacgcg tatacggctt gactttagat gtcggaatct
     4561 tcttgaatat ctcgcctgac catatcagtc cgatcgaaca tccgacattt gatgactatt
     4621 tctattgcaa gcgacaattg atcttgaatt caaaaacagt tgttttaaat catgaaagtg
     4681 actattttga tttacttagg gaaacagccg atctattcca gattccaacg atcacttatg
     4741 gtcgttcgga agaaagcaat tatcaagtga tccgttcaga taaaggtact cacggtttta
     4801 ctttatcttc tcatgaagat cgcttagcta tctgcgatac tgcttatgat attttgctgg
     4861 ctggaggatt caaccaagaa aatgcagcca gtgcgatcat tgccgcagca ctaacaggtg
     4921 cctccagtca agatgcacaa gacggattaa aagaagctcg tgtaccaggc cgaatggatc
     4981 aattgatcca atccaatgga gcgcatgtct atgtggacta tgcacataat tatctaagct
     5041 tgaaaacatt gctgaaattt gccaaaaatg aacatccaga tggccgtgta attgttgtat
     5101 taggcagccc aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa gtgctatcag
     5161 aaacagctga catcgccttt ttgacggcag atgacccagc atttgaagat ccgaaaaaaa
     5221 tcgcggaaga aatcaatgaa gcaattacta atccagattt gatcgttcat tatgaaatgg
     5281 atcgtcctga agcgattcga cgcgctttag cagaaagcgc tccgcaagat tcagtcgtta
     5341 ttgcgggaaa aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa ccttatgaag
     5401 gggattatgc aatcgtgaag cggttgattg aagaataatg gtatttatat actagtggtt
     5461 tcccatttct aagaaaaaaa gggactgtga ttttttgtca cagttccttt tttcttagaa
     5521 atcattttaa cttttcacta gctaattctg ctgccttttc tctttcttca tttaataatt
     5581 tattatcata gaatttgata aatgggaacc agataatgaa tgcgatgatt gcacaaatga
     5641 ctgctaacac agcagctttc caatcaccac cactaccaac aaatgctccg atcccgactg
     5701 gtgaaggcca cggcatctgt gcaagcatcg gtttgacgat ttccagccga atggcaaaat
     5761 aagcgagtgt agcagaagcc atcggagcta agaaaaacgg aaccgctaaa tagggattgt
     5821 agacgattgg catcccgaaa atgattggtt cattgatatt gaataatcct ggtacaagtg
     5881 aggcttttcc taacactttc agctgatcag atttcgcgag gaatgcaata aagatgatca
     5941 atcctaaagt cgcacctgat ccaccaacgg ttacataaga attgttgaat tctcctgcca
     6001 acggataatt tgcgccttgt gcgttagctg ccatattagc taatacgatc ggggtcaaga
     6061 aagaagtgat gatattcgct ccatggatcc ctacgatcca caatgcatgg atcaagaaat
     6121 aaatcaccat gacaccaagc catgtattcg tcaactgcgt aacaaaccca aatgggatag
     6181 caatcatttt gaagatatcc gttcctagtg caactaataa tccattgatg atcatcacca
     6241 caaacgcaat gacaaatgtt gggatcagag cagtgaaaga acgagaaact cctggcggaa
     6301 caacatctgg catttttatg acccaatttc tcttgacaca catacaatac aattcgaccg
     6361 caataatcga cataataata gctgtaaaga ttcctgaagt tcctaaacgt tcgacaaaag
     6421 agcccatcct aataccattt actacagttt ctgtctcatt gatagaagca atcaagctga
     6481 ttttaccatc agtcatgatc aattcaggga tacacataaa gaatgcaaaa acagatagta
     6541 atgcccccgt gagcggatta acatttaatt tttgttcatc tcgttcaatt gacgttaatt
     6601 cataagcaaa cacaatggca aagtaaatag ataaaatccc catcgtcatt gtattgacga
     6661 tcatgtacaa gtcactaact ttgaaaaaag ttgcattaaa aaatccttct aaaaatggga
     6721 acggtaatgg caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg aaaggaatag
     6781 aagccatccc cgctgccatg acagcccgaa cgattttaag ttgcgcaatt ttccccatag
     6841 gtcccatcag gtatttctgc aaaaaatcaa acattagtta ttctcctttc ctaaatataa
     6901 cgctttttca tattgtttaa tacggatata ctgttggtct tggttgagtg aaattttctt
     6961 taaacgatac tgtataaatg cactggcaaa aataccgctt acaacaagaa tgaaaacaaa
     7021 taactgtcct ccattgttat ttgccataaa aggatacaag aatgtaaatc ctgaggtaaa
     7081 caacactgga atcactaata aattaaagac actctggaac aaaaaatacc actttgtcca
     7141 tggaacgttc gtttggtgtt ttcgataaag ctttacttgt tctcctactg caggcaaaat
     7201 agctaaaatc agaatcatcg gtaaactact tcctaaactt ttagctgata gaagcagaat
     7261 catccattgc atgttcatga aaaagaaaaa ggcagtcagg taacgtacga ttagataacg
     7321 attgaaatac atgtttttca aactaagttg ctttttttct aattcgattt tcctttttac
     7381 tgtatcctgc acaatgatcc ttcctttcta tttatttttt tgatacaaat aattcatttc
     7441 ctgtgcaact tccaataatg tcatcgtcgt catcagatga tcttgtgcat ggaccatgat
     7501 aatttccatt tctatttttg tcccgctggc atattctttc agaagttgag tttgtgactg
     7561 atgcgcatga agaatctctt catttgcttg tcgcaacttc tcttctactt cttcaaacat
     7621 ctctttacgc atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc cgctatggaa
     7681 aatgatatca aaagaagtca cttgtagtgt ttcactgttt attttttcag ccatctgttt
     7741 tcctccttat tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa taggttcagc
     7801 taagatttcc tgctgtactt ttagctggtc aactaaccta acgattgctt tagtcacgac
     7861 cgtaattcct tcattttcga cataagaagg cgaaactaaa aaaacaaaat gaatttcttg
     7921 atattgatcc cactgcatcc cagaaggaat caatgcaacg gctacctttg tttcgattcc
     7981 aactggaaca gcgggatgtg ggactgccac tgtatcactg aatatgattt gtcccatatt
     8041 ttcacgttgc tggatctgct ttcgcatttc tcttatatat ccttcttctt catttatgct
     8101 cagcttttgc agcaaatcat gaagaatttc ttcctttttc ggttcgtacg tataagtctt
     8161 gaaaaatttt tcagacaact gttcggtgta aatctgcttt ttttgctgta gagaaaatgc
     8221 tggtatcggt ctatctgagg aatgataggg tatagattct ccaactacct ttcgtatttt
     8281 acgaacatca tcttcattca aaaaaatact gacatgtaaa actggaattt tgaaaaacat
     8341 tgtcgaaaga tcaatagaag aaataatcag gtcgattcct tttaaagttg tttcattgat
     8401 ctcataatag ccttttacac ttaccactgt aatattttta ccgaattcac tgactacgcg
     8461 attctttaat agttgggcac tgccgtaacc cgttgcacag ataatcaatg catgtagttt
     8521 atgcgcagct ctttcttttt ccaatgccgc catgagatgc aaagccaaat aggcccattc
     8581 atcttcattg atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc tttttactgc
     8641 gtcaaagact ttgggatttt ctttctttat ctcctcagtc aatgggttct caatgcgaat
     8701 acctcggctc aatcgaatca tcatcggtga caaatgatcg agtagtccat tgaaaagctg
     8761 gagatcctct actaatgaaa aatgaaattc ctgagacaat cttttcagaa ctttcgctaa
     8821 ttcctttgat agttcctgat cattttgatt ttctttgtga ttcgacttag ccatgagatg
     8881 taatgtcaaa taagcaattt cttcttcggg aaacgataca cctgcgatta cctcaatacg
     8941 ttgaacgatc cgctgcgcca cttgatactc tactcgctta gaaacttctt gttcgatgcc
     9001 taattccttg atttcaaacc cttctcggag ccgtttgatc cccaaagcca agtgtaaagt
     9061 cagattctgt atgataaaat ccgaaacttt taacttagct tctcttgttt catcgagaat
     9121 gataatgact agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc ctaaatattc
     9181 tttcaatgaa tttccataat ggtttccaaa aaaagtatcc atgataaaat gccgtcgatt
     9241 tctttcttcc ccttcgatcc atgtcccttg accatgctta gaaacgattt ttagtgaata
     9301 tggaatcaat cgctccttga tttcttgcat gtcctttgtc aaactggaac gactgatata
     9361 aagctcttct gccaaatcat ccataaaaag aatggcatct tccaaaagca attggttcaa
     9421 tatatacttt tgtcgatctt cggactcata aaatccttgc ggttctctag catatccagg
     9481 tactatttct tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa tatgcagttg
     9541 gtagccataa cctggtttag caatgatctt acctccgttt ttttctacta actctttgat
     9601 ccgctgcaag taattacgga ttgtccgatc agataataac aattcagaag ctaaacgttg
     9661 actggtgaca tactccttct tgtgatcaat caaatatctc aacagcaatt tttccttctg
     9721 tttcattatc atccactcct attccatcat atgttctctt cgatcacctc tgctaacttc
     9781 tggatgcctg tagggattgg gacatacgct tggaaaggga tactaacaac aggctttcca
     9841 acagattctc ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg gctgatcaga
     9901 aataaatcaa aatcactatt cttaatcata ttatcgcctt ctgtagctga aactgcatct
     9961 acgagtacct cttctctttt ttgcttaaag aaatccgttg tctttgaggc catcatcgag
    10021 gatgacattc ctgccgcaca aataatcaat gcttttttca taaatcaatc actcctttta
    10081 tttttataaa ccaagcatat cttgtaagcg ttttctattc aaaatatata tttccgtagc
    10141 attgcggaaa aaatgtgtat catatcttga ataataattt ctggcaccgt gatatactag
    10201 tgatagaatt ttttcaggag gggatttagt gagtatcaca gtgaatattt tgtatacaag
    10261 tacaaatgga caagccagaa aatttgcaga agaaatggtc gaaagaaaca ttgtcaaagc
    10321 gattcgtgca gaagaaggaa acgagaaata tgactattac tttccactag aagatccaga
    10381 atctctttta ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc atcacaaatc
    10441 cgaaatgatg gctcaaataa acgaactccg taaaaagtat aaattaaaaa tgaaagtcga
    10501 acgtttcact gatttaccgt aaaaagatac acatggaatg atagcaattt atcatccatg
    10561 tgtatcttca ctttttcctt attttttatt cgatctcatc taatgattca aaccgtgttt
    10621 ttctcttatt tttcggataa gtatttcagt caccccaata tacatgattc ctaaacatac
    10681 cccaaggata ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga ataaactggc
    10741 agtgattgcc aaagcaccaa aagaattaaa aactgtttta tatctatagg tagaacaaac
    10801 acctaaagct agaccaccta atataccgac ccagttaaaa ggaataaatt ggctgatacc
    10861 aataaataaa acacatccaa tcaatgagcc aatgattcgg tctactgctc gttctttgaa
    10921 accaatcgac atcaatccat agctagatac aatagaagaa aatgcaaacc ctgcccacat
    10981 caatcgctga aaagggacat actctcctgc aacgatcaaa agactaatgc caaatgcata
    11041 gatgagcatc caaatttttt cctgcgaata catgcctttc aaaaaaatct ctttaaacaa
    11101 gcttttgtct ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa accaacaaaa
    11161 aaacaaaaat aataagctac ttgtttgtgt aaagaaattc ttgttcaacg aatctttagg
    11221 taacgaataa acaataaaga gatatgaaaa gccgtacaaa gaagcattcc ccattttggg
    11281 atcactagcg gttgccatta atatgctgct tagtaaaata aaatgaatca acatgcccat
    11341 aactgaccat ttgatcagct ggacatacgg tgctactaat agtgagaaca tgacgattgc
    11401 taaacctatg atcgaatggg aaatcttata accaaagtca acaaaacgaa tactcatcaa
    11461 catacagaat aaaaccacag cgtatggttt atgcttcgat tcaaataatg ttgtaattat
    11521 aatgataaaa caaatggcaa acaatacgat gaataatgat cgtaaaaaca atgaacaaat
    11581 atacatgcac ttttcttttt tagaagctgc ttctcgtatt ttttgtttta aaataaacgg
    11641 atctagttgt agagactgat aaaaatccac ctgttgtcgt cctttctttt tacttttatt
    11701 ttttacagtc atctatgaca ggttgtaaat actatggaac atcatatggc gttgtcattt
    11761 tcttgtcaac tatttttcct attcatgttt ttataaaaaa acatatcgtt ttcttgtatg
    11821 attaaatcac cacaaaataa acaactgaga gaacgatatg ctttatgaat ggttctatca
    11881 aaaaaattct gagatcaata gacaaaaatt tgatgattac agaggtctct tacgaaacct
    11941 tacagaagaa aaaaacattg atcgttgctg cagttctctt ccctgctcca tgtgtctgta
    12001 gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt aatggtggtg aaaaacaaga
    12061 agaaggaaac cattgtccac tttgaataat ataatcatat gccaatggtt atacgcctaa
    12121 aaaagcagcg ctatacctgt aaaaactgtc gaagccattg gaacgctcaa agttatttta
    12181 tccgtccaag gcactcgatt tcaaatcatg tcagacataa aatcacttct ttactgactg
    12241 aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt atctttgaca accgttattc
    12301 gcacattgaa agagttgaga agctatttac caaagtcttc caaaagaatt ctgtcttgag
    12361 tgttgagggt tgtgaatttc gttctcacgt ttccgttgag gataaactga gttttatttc
    12421 cgcggatggc gagacagaaa aattaatcga gaaaatttac ctacacaaaa aattcctcga
    12481 ttaacaaact atttccttag ctgcactaat cctaaagaaa tagaattctt agttacagac
    12541 atgaacgcca cctactttca gctcaccaaa catgttctac caaatgcgaa attggtgatt
    12601 gatcgattac ttggtttttc cacgcctttc aaggaggcgt aacctttatt cacgagttgg
    12661 ttgaagcttt ccgagacaaa agacccgact tgtttttctc cttattgaca aagagccatt
    12721 aaaactagag aaaaaattac aactgatcgc tgattatttg tagaaagaaa atcaaagtat
    12781 tacccaataa acttgagtca ttaggcaaaa aacactgcga aactcaagaa tgattttcgc
    12841 agtgtttttg tatatttttt attaactagt attactacaa aggtttattt ttcacttctc
    12901 aacgcagcac aaaattgtct ttacttattt actggttggg ttcaaccttc tctactccga
    12961 tactaatatt tgtttgtata ccccttctaa aatcggtagt ttccattcaa ttgatgggta
    13021 ctagttgatt ttaaaaacta tgcttattca attttttatt ttagctcata tatttcgttt
    13081 ctttctaaat tttatactga tgaaaacaag tagcacaccc aatattgaca atataaactg
    13141 gctaatgatg gtttctccag tttttggaag atgcttattt ccactgcttg ttggattgtt
    13201 tcctcttata tctgagctat cagcaacttt tctattctct tttgttaaaa agatcgccac
    13261 ttcttgttct ttttcgatct tgaaagttac tggtgtcgca tccaattcat accccgttgg
    13321 cgcttgagtt tctaccagct gatatgctcc tggtgctaag ccatctatcg ccagttttcc
    13381 atctgctcct gtggttagac ctgactgcag aacggttcca ttggcatctt gtaactcaaa
    13441 aacagctcct tgcaggactt ctccgctttg gtcatcgatc tttgtcaaca cgacaccgcc
    13501 tggtgtaagt cgattttctt tcgtcaattc taccaccgct gtctgacttc tttcgatctc
    13561 aaactcgatt ggtgtcgcat ccaattcata ccccgttggc gcttgggttt ctaccagctg
    13621 atacactcct ggtgctaagc cgtcaatcgc cagttttcca tctgctcctg tggttagacc
    13681 tgactgcaga acggttccat tggcatcttg taactcaaaa acagctcctt gcaggacttc
    13741 cccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc ggttttcttt
    13801 cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgatcg gtgtcgcatc
    13861 taattcatac cccgttggcg cttgagtttc taccagctga
//
LOCUS       DARBER010000061        13807 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DARBER010000061 DARBER010000000
VERSION     DARBER010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.062"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SCK06_001696"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SCK06_001696"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG1151695.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="SCK06_001697"
     CDS             complement(2260..2511)
                     /locus_tag="SCK06_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG1151696.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="SCK06_001698"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="SCK06_001698"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG1151697.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="SCK06_001699"
     CDS             3461..3679
                     /locus_tag="SCK06_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151698.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="SCK06_001700"
     CDS             complement(3968..4480)
                     /locus_tag="SCK06_001700"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG1151699.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="SCK06_001701"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="SCK06_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG1151700.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="SCK06_001702"
     CDS             complement(6933..7376)
                     /locus_tag="SCK06_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG1151701.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="SCK06_001703"
     CDS             complement(7377..8165)
                     /locus_tag="SCK06_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1151702.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="SCK06_001704"
     CDS             complement(8162..9103)
                     /locus_tag="SCK06_001704"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG1151703.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="SCK06_001705"
     CDS             complement(9280..9867)
                     /locus_tag="SCK06_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG1151704.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="SCK06_001706"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="SCK06_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG1151705.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="SCK06_001707"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="SCK06_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1151706.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="SCK06_001708"
     CDS             complement(12784..13719)
                     /locus_tag="SCK06_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1151707.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DARBER010000062        13682 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARBER010000062 DARBER010000000
VERSION     DARBER010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.063"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            137..475
                     /locus_tag="SCK06_001709"
     CDS             137..475
                     /locus_tag="SCK06_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151708.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="SCK06_001710"
     CDS             complement(680..1255)
                     /locus_tag="SCK06_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG1151709.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1987..2568)
                     /locus_tag="SCK06_001711"
     CDS             complement(1987..2568)
                     /locus_tag="SCK06_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1151710.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2751..3377)
                     /locus_tag="SCK06_001712"
     CDS             complement(2751..3377)
                     /locus_tag="SCK06_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1151711.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3399..4730)
                     /locus_tag="SCK06_001713"
     CDS             complement(3399..4730)
                     /locus_tag="SCK06_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG1151712.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4885..5286)
                     /locus_tag="SCK06_001714"
     CDS             complement(4885..5286)
                     /locus_tag="SCK06_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1151713.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5372..5578)
                     /locus_tag="SCK06_001715"
     CDS             complement(5372..5578)
                     /locus_tag="SCK06_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151714.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5673..7175)
                     /locus_tag="SCK06_001716"
     CDS             complement(5673..7175)
                     /locus_tag="SCK06_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG1151715.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7193..7648)
                     /gene="argR"
                     /locus_tag="SCK06_001717"
     CDS             complement(7193..7648)
                     /gene="argR"
                     /locus_tag="SCK06_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1151716.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7746..8363)
                     /locus_tag="SCK06_001718"
     CDS             complement(7746..8363)
                     /locus_tag="SCK06_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG1151717.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8584..9957
                     /locus_tag="SCK06_001719"
     CDS             8584..9957
                     /locus_tag="SCK06_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1151718.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10032..11144
                     /locus_tag="SCK06_001720"
     CDS             10032..11144
                     /locus_tag="SCK06_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG1151719.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11216..12853)
                     /locus_tag="SCK06_001721"
     CDS             complement(11216..12853)
                     /locus_tag="SCK06_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG1151720.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13021..13629)
                     /locus_tag="SCK06_001722"
     CDS             complement(13021..13629)
                     /locus_tag="SCK06_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1151721.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3687 a         2883 c         2452 g         4660 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     1681 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     1741 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     1801 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     1861 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     1921 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     1981 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2041 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2101 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     2161 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     2221 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     2281 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     2341 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     2401 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     2461 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     2521 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     2581 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     2641 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     2701 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     2761 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     2821 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     2881 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     2941 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3001 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3061 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3121 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     3181 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     3241 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     3301 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     3361 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     3421 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     3481 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     3541 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     3601 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     3661 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     3721 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     3781 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     3841 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     3901 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     3961 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4021 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4081 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4141 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     4201 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     4261 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     4321 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     4381 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     4441 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     4501 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     4561 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     4621 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     4681 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     4741 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     4801 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     4861 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     4921 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     4981 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5041 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5101 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     5161 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     5221 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     5281 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     5341 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     5401 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     5461 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     5521 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     5581 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     5641 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     5701 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     5761 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     5821 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     5881 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     5941 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6001 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6061 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6121 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     6181 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     6241 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     6301 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     6361 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     6421 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     6481 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     6541 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     6601 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     6661 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     6721 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     6781 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     6841 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     6901 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     6961 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7021 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7081 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7141 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     7201 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     7261 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     7321 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     7381 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     7441 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     7501 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     7561 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     7621 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     7681 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     7741 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     7801 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     7861 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     7921 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     7981 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8041 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8101 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     8161 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     8221 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     8281 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     8341 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     8401 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     8461 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     8521 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     8581 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     8641 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     8701 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     8761 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     8821 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     8881 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     8941 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9001 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9061 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9121 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
     9181 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
     9241 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
     9301 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
     9361 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
     9421 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
     9481 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
     9541 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
     9601 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
     9661 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
     9721 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
     9781 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
     9841 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
     9901 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
     9961 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10021 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10081 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10141 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    10201 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    10261 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    10321 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    10381 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    10441 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    10501 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    10561 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    10621 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    10681 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    10741 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    10801 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    10861 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    10921 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    10981 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11041 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11101 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    11161 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    11221 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    11281 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    11341 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    11401 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    11461 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    11521 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    11581 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    11641 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    11701 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    11761 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    11821 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    11881 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    11941 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12001 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12061 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12121 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    12181 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    12241 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    12301 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    12361 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    12421 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    12481 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    12541 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    12601 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    12661 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    12721 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    12781 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    12841 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    12901 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    12961 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13021 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13081 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13141 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    13201 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    13261 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    13321 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    13381 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    13441 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    13501 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    13561 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    13621 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    13681 at
//
LOCUS       DARBER010000063        13647 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARBER010000063 DARBER010000000
VERSION     DARBER010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.064"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            74..316
                     /gene="yaaA"
                     /locus_tag="SCK06_001723"
     CDS             74..316
                     /gene="yaaA"
                     /locus_tag="SCK06_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG1151722.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            303..1427
                     /gene="recF"
                     /locus_tag="SCK06_001724"
     CDS             303..1427
                     /gene="recF"
                     /locus_tag="SCK06_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG1151723.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1424..3370
                     /gene="gyrB"
                     /locus_tag="SCK06_001725"
     CDS             1424..3370
                     /gene="gyrB"
                     /locus_tag="SCK06_001725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG1151724.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3390..5861
                     /gene="gyrA"
                     /locus_tag="SCK06_001726"
     CDS             3390..5861
                     /gene="gyrA"
                     /locus_tag="SCK06_001726"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG1151725.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6097..6396
                     /locus_tag="SCK06_001727"
     CDS             6097..6396
                     /locus_tag="SCK06_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG1151726.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6446..6958
                     /gene="ssb"
                     /locus_tag="SCK06_001728"
     CDS             6446..6958
                     /gene="ssb"
                     /locus_tag="SCK06_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1151727.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6984..7220
                     /locus_tag="SCK06_001729"
     CDS             6984..7220
                     /locus_tag="SCK06_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG1151728.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7363..9336
                     /locus_tag="SCK06_001730"
     CDS             7363..9336
                     /locus_tag="SCK06_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG1151729.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9342..9794
                     /gene="rplI"
                     /locus_tag="SCK06_001731"
     CDS             9342..9794
                     /gene="rplI"
                     /locus_tag="SCK06_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG1151730.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10068..11435
                     /gene="dnaB"
                     /locus_tag="SCK06_001732"
     CDS             10068..11435
                     /gene="dnaB"
                     /locus_tag="SCK06_001732"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG1151731.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12215..13507
                     /locus_tag="SCK06_001733"
     CDS             12215..13507
                     /locus_tag="SCK06_001733"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG1151732.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4549 a         2467 c         3002 g         3629 t
ORIGIN      
        1 gataaatttg ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa
       61 tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca
      121 aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga
      181 aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg
      241 catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga
      301 taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg
      361 aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt
      421 tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat
      481 tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca
      541 ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg
      601 aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag
      661 atctttcttt agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag
      721 gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa
      781 gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata
      841 ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg
      901 tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc
      961 agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa
     1021 tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa
     1081 caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc
     1141 agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa
     1201 tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga
     1261 gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa
     1321 cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga
     1381 ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag
     1441 tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga
     1501 agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca
     1561 ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa
     1621 aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat
     1681 acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt
     1741 acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt
     1801 aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg
     1861 aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga
     1921 acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga
     1981 gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa
     2041 ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata
     2101 ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat
     2161 ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat
     2221 gcaatataca gacggctacc attccaatat catgagtttt gccaacaata tccatacgta
     2281 tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta
     2341 tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg
     2401 agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac
     2461 aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca
     2521 tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt
     2581 actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg
     2641 tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga
     2701 aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg
     2761 taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc
     2821 aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac
     2881 agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac
     2941 tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta
     3001 catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt
     3061 aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa
     3121 acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact
     3181 tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat
     3241 ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg
     3301 ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt
     3361 agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg
     3421 atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg
     3481 tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat
     3541 acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg
     3601 tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg
     3661 tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg
     3721 gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa
     3781 tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg
     3841 atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg
     3901 gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg
     3961 tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg
     4021 aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc
     4081 gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg
     4141 agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag
     4201 caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag
     4261 atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg
     4321 ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg
     4381 gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc
     4441 ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag
     4501 aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg
     4561 atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga
     4621 tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc
     4681 gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga
     4741 ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg
     4801 aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg
     4861 aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga
     4921 caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg
     4981 gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt
     5041 gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag
     5101 gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag
     5161 gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag
     5221 gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt
     5281 ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg
     5341 tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat aacggctact
     5401 ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag
     5461 gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag
     5521 aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag
     5581 tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac
     5641 tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg
     5701 tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac
     5761 gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag
     5821 ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt
     5881 aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt
     5941 tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag
     6001 tgtataatca gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa
     6061 cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc
     6121 atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc
     6181 gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc
     6241 cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct
     6301 tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat
     6361 atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag
     6421 aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac
     6481 gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc
     6541 tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt
     6601 gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg
     6661 tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt
     6721 gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg
     6781 ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa
     6841 taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc
     6901 tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac
     6961 gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta
     7021 aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac
     7081 taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg
     7141 ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac
     7201 ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct
     7261 attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa
     7321 agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat
     7381 catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc
     7441 ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt
     7501 gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag
     7561 caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga
     7621 gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa
     7681 atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa
     7741 gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca
     7801 gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag
     7861 acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga tgccattgat
     7921 aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac
     7981 tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta
     8041 gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt
     8101 cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc
     8161 tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc
     8221 cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat
     8281 ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc
     8341 gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg
     8401 gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa
     8461 aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa
     8521 gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag
     8581 ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc
     8641 tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg
     8701 gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca
     8761 gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt
     8821 gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca
     8881 actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta
     8941 gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct
     9001 ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat
     9061 aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca
     9121 gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc
     9181 acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca
     9241 gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc
     9301 aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac
     9361 aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac
     9421 aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt
     9481 tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa
     9541 agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg
     9601 aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac
     9661 aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag
     9721 gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg
     9781 tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa
     9841 aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa
     9901 atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac
     9961 gagtataata aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct
    10021 ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt
    10081 ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt
    10141 ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc
    10201 gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga
    10261 ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg
    10321 gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact
    10381 atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa
    10441 tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac
    10501 gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag
    10561 tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag
    10621 gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac
    10681 taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc
    10741 aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag
    10801 aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta
    10861 caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc
    10921 gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat
    10981 gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt
    11041 tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac
    11101 tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg
    11161 gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa
    11221 ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag
    11281 gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg
    11341 agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata
    11401 agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa
    11461 tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa
    11521 tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat
    11581 aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa
    11641 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt
    11701 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga
    11761 gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc
    11821 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat
    11881 atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
    11941 cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa
    12001 aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta
    12061 caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata
    12121 aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg
    12181 ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac
    12241 gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt
    12301 gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac
    12361 ttataaactg catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg
    12421 aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa
    12481 aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca
    12541 tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat
    12601 taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga
    12661 cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg
    12721 tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata
    12781 ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga
    12841 tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat
    12901 cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat
    12961 cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac
    13021 gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat
    13081 ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt
    13141 tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa
    13201 ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga
    13261 tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat
    13321 ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact
    13381 gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc
    13441 aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca
    13501 aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt
    13561 ttatctatac gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa
    13621 aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DARBER010000064        13429 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARBER010000064 DARBER010000000
VERSION     DARBER010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.065"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..914)
                     /locus_tag="SCK06_001734"
     CDS             complement(<1..914)
                     /locus_tag="SCK06_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG1151733.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="SCK06_001735"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="SCK06_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG1151734.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="SCK06_001736"
     CDS             2006..2176
                     /locus_tag="SCK06_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151735.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="SCK06_001737"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="SCK06_001737"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG1151736.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="SCK06_001738"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="SCK06_001738"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG1151737.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="SCK06_001739"
     CDS             4440..5321
                     /locus_tag="SCK06_001739"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG1151738.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="SCK06_001740"
     CDS             5318..6034
                     /locus_tag="SCK06_001740"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG1151739.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="SCK06_001741"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="SCK06_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG1151740.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="SCK06_001742"
     CDS             6751..7752
                     /locus_tag="SCK06_001742"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG1151741.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="SCK06_001743"
     CDS             7749..9110
                     /locus_tag="SCK06_001743"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG1151742.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="SCK06_001744"
     CDS             9070..10221
                     /locus_tag="SCK06_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG1151743.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="SCK06_001745"
     CDS             10196..11365
                     /locus_tag="SCK06_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1151744.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="SCK06_001746"
     CDS             11540..12919
                     /locus_tag="SCK06_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1151745.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="SCK06_001747"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="SCK06_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DARBER010000065        13342 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARBER010000065 DARBER010000000
VERSION     DARBER010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.066"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            13..159
                     /locus_tag="SCK06_001748"
     CDS             13..159
                     /locus_tag="SCK06_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG1151746.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(221..2032)
                     /locus_tag="SCK06_001749"
     CDS             complement(221..2032)
                     /locus_tag="SCK06_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG1151747.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2029..2394)
                     /locus_tag="SCK06_001750"
     CDS             complement(2029..2394)
                     /locus_tag="SCK06_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG1151748.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2557..2952)
                     /locus_tag="SCK06_001751"
     CDS             complement(2557..2952)
                     /locus_tag="SCK06_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1151749.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2970..3932)
                     /locus_tag="SCK06_001752"
     CDS             complement(2970..3932)
                     /locus_tag="SCK06_001752"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG1151750.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3932..4144)
                     /locus_tag="SCK06_001753"
     CDS             complement(3932..4144)
                     /locus_tag="SCK06_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG1151751.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4165..4863)
                     /locus_tag="SCK06_001754"
     CDS             complement(4165..4863)
                     /locus_tag="SCK06_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG1151752.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4883..5425)
                     /locus_tag="SCK06_001755"
     CDS             complement(4883..5425)
                     /locus_tag="SCK06_001755"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG1151753.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5557..6561)
                     /locus_tag="SCK06_001756"
     CDS             complement(5557..6561)
                     /locus_tag="SCK06_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1151754.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLTLIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6558..7547)
                     /locus_tag="SCK06_001757"
     CDS             complement(6558..7547)
                     /locus_tag="SCK06_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1151755.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7544..8350)
                     /locus_tag="SCK06_001758"
     CDS             complement(7544..8350)
                     /locus_tag="SCK06_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151756.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8516..9472
                     /locus_tag="SCK06_001759"
     CDS             8516..9472
                     /locus_tag="SCK06_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1151757.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9549..10067)
                     /locus_tag="SCK06_001760"
     CDS             complement(9549..10067)
                     /locus_tag="SCK06_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1151758.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10155..10304)
                     /gene="rpmG"
                     /locus_tag="SCK06_001761"
     CDS             complement(10155..10304)
                     /gene="rpmG"
                     /locus_tag="SCK06_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1151759.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10532..10978
                     /locus_tag="SCK06_001762"
     CDS             10532..10978
                     /locus_tag="SCK06_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG1151760.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11173..13068)
                     /gene="asnB"
                     /locus_tag="SCK06_001763"
     CDS             complement(11173..13068)
                     /gene="asnB"
                     /locus_tag="SCK06_001763"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG1151761.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4150 a         2831 c         2276 g         4085 t
ORIGIN      
        1 tttgtatctg gaatattagt aggaagtctg gcaacagccg ctgccgttgc tggtttggtt
       61 gcttctgtga aaaaaacagt gatcgatcca attgacgaaa aagaagcaat gatcgaagaa
      121 aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat aattatttaa aaatacgcat
      181 gaagactttc taaaaaacag ataagtcatc atgcgtattt ttatttgtca gtcaatataa
      241 gttgtgctat tttatctgcc tgtctcagat tccatggatc tgtataaggt ctagctactg
      301 ctgcagttag ccaatccaca ccttcaacag gtattccata gcaataaaag tcagactgga
      361 tttcccctga agcatcaatc acgcgattcg tacctcgctc aacatctata gcttctgaat
      421 gataacttcc tgtattatct gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat
      481 tttttatgac aggattactt gaacgagaaa agcttgtttg tggcaatcga gcttcaatca
      541 aatagcgact gctgatttct ttatcttgta gagaaataac atagcggttg tgcttttgct
      601 gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc atctatcagt gctgctaatt
      661 ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct atttagatga gtaaaccatc
      721 cccatagtat ttctttgtat tccttagcag aaaaaacgtg ccattcaata gcctgccgga
      781 ttggatcacg ccagtctttt aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc
      841 ctttttccgc ttcttttatc tgcccttcaa taaatattcg gctagcttct gaaaagggct
      901 cattctgata ggcaagagga gtttctaaat acgaccaatc ccatctataa gggattagtg
      961 ctggatgctt tccgagacac tcctctgaag agagggagag aaaatcatgc tcaaaatttt
     1021 tctttggaat cggcaagtga tattcttcga tgattttctt gtaataaaac aactcaacgt
     1081 cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga tgccattgct
     1141 ccaggttttc ttttgttagt aaccgtggcc atgccattgc tccgccttgc ttttgatttc
     1201 ttcctcgagg gtaataaggg agcccccttc ttgatccact gtatacaatc ggttccttgc
     1261 ctgatggatg atagaccagt tttccctttt cgttctcgaa gcgccctcct cttgatactg
     1321 taaacaatcc gacataatca aaaaaactta agcctagacc tcttaaaaag acattttctt
     1381 taggcggtat cttcgaaaga tctacatctg caggattaga aggaagctga tacagtaaaa
     1441 catttttctt tgcgtagtct acccacactc tttcttcttc tgtctcatca ttttcccagt
     1501 gaccgcttgc taaaacaagc tgatcgatag aatagctttg ctgatctgtc tgtaaaagat
     1561 aaccatcttt tcttttgctt acttttttta ctaaagaata aacaaactga atagaagctg
     1621 gaaattcttc ttgcaacttt gagaaaaacc agcgttgata gagtccgtat aaacatcgag
     1681 tagattgctg atctttttcc agacgattga tttcttctat aattagttgc ttatttttgg
     1741 ttaccgtctg ttcgatataa tctgtagcag gtcctttact ccattgaaac aagttgggtc
     1801 cctgtgttac caatcctcca ctggacaatg tttcatctgt aaaaagcgtc acttgttgag
     1861 aaacagaatt cattaataac tccggtggct gattcaaccg ccaaactcga cctccaggac
     1921 cattgggatc gaaaagaaaa agttcaagag aatgtctgtc ctgattatta tggagaatcc
     1981 tttctgctac acttaatcct ctaggtccgg caccaatgat ccctattttc atttgcatgc
     2041 ctccatttcg ataaattttt tcaataaaac agcatggttc tgatgttcat tttttgcacc
     2101 ataaacaaaa gtaatgactg gatgttcttt cataagttga ctgagtttaa aaatagcttg
     2161 cttcttttct tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg
     2221 ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca
     2281 cagatctaac tgcgcttttt cttttgaaat tcctcgtggc cagaatctat ccacgagtat
     2341 tctatagcca tcctcttttt cagcatcaag gtaaacacgt tttaatttga tcatagttgt
     2401 ttccttcttt ctaccttttc gcttagctta tatatgtaga taatagcaca aaataaaaaa
     2461 aatgaacggc acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt
     2521 gattcggttt ttaaagctgt catacaatca tctgttttat acttttttac ttttgatttt
     2581 tccagaccat ccgtcatatc cacccttaag taaataaatc tcttgataac ctgctttacg
     2641 taatttattt gctgtacgga tgctcaaact tttcttttga tcgtaaatat agacaggctg
     2701 gtcttttcga atagatccta atgaatcttt caaaacagtg taagggatgt ttcttgcacc
     2761 taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca tcgattactt gtgcttttct
     2821 cattccttct cgaaattctt cttctgtcaa agtcgttgct gaacgtttgg ccattactcg
     2881 tagatagact tcccaaagga tgattgcaag cacaatcaat agcaaaatga tattaattac
     2941 ccataaaacc atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa tgaggcagca
     3001 ccaatgacac cagcttcatt accaagctct gctaatttga tttttgttga ttctcttact
     3061 tgcgggaaag taaattcttt aaaatatttc tctacacggc tacgtaaaaa ttctcctgct
     3121 gccgatactc ctccacctaa cacgatactt gacggattta atgtattccc caagtttccg
     3181 caagctaaac ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct
     3241 tcttgagcca tttcaaagat atctttactt gttacttctt ctccgttgtc tagcattgct
     3301 ttcagttttg aatcccctgc gtattcttct gccaagaaac gacctaggcg taccacacct
     3361 gtagcgctag atacagtttc taaacatccg cgtttaccgc aagtacattc gaacccttca
     3421 ggatccacag tgatatgtcc gatttctcct ccagctccgc tcacgccgtg aatcaaattt
     3481 ccgccagcaa cgatacctcc gccaacacct gttcctagag taacgaatac aacgtctgga
     3541 tcgttttctc cagcaccttt ccatcgttca cctaaagcag caacattcgc gtcattatcg
     3601 atagcgaatt tgattcctgt tcctgcctcg atcagttttt tcacaaattg aacttctgtc
     3661 cagtttaagt tatacgcacc aataaccgtt ccagcttcgc tgtccacgct tcctggggta
     3721 cccatgccga ttccgataaa gtcttcggct ttcatattat ataaattcaa acgatgattg
     3781 attgattcaa tgatttcagg aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg
     3841 ctccattttt gctgaatctc tccttctggt gttaaaattg caaattttgc tgttgttcca
     3901 cctaaatcaa tcccaataat cttcttgtcc attacaaatt tcctttcttt cggctttctt
     3961 caattcggtg ctctcttttt aatatgcgcc acgctgtcat gtatgtatca tgatcaatca
     4021 aacggctatc atacagatta cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg
     4081 cacccacata gataaagatg ccaaattttt tgaacagctg ctgtacatca tataagctct
     4141 ccatctttaa gcacttcctt catattatac aagtaaacca tattttttta aacccagtaa
     4201 aatacatatt acaaaaagaa atacaaaaac tagtgtagaa agaatccttt ggtgtatcga
     4261 atagcttgtc tgttgtttcg gtagacccaa aacattccct aacactaatc cgccaattag
     4321 cccaccgaca tgtccccaaa catctacaga ggtatccaga agaccaaaaa caaatgtcaa
     4381 cacaataaat aaaagaaaat gtctgactag ctgctggatc gctgggttat gtttaaaatg
     4441 aaaccctaag ataaacaacg caccgaacaa accgaatatc gaagtacttg caccaccaga
     4501 cagaacattc gcttcgttaa aagcaaagct tgctgtattt cccattatcc cgctgaacat
     4561 ataaatcaag aaaaaacgcc aatggccata gattgcctcg atttgctgcc ccataaaata
     4621 cagtatcagt gagttcatta cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac
     4681 taaacgccac cattcattga agtgaacaag ataaggacca tacattccgg cataataagg
     4741 gatctcaagt cgcggaaata attccatcat gaggaagacc gctgtctgta tccctaaaaa
     4801 agcataggtc cataacggtc gtcttaacca tgcacgcaat tttatttgct gttgataatt
     4861 catgaaaacc tcccatttca ttttagtcaa taaacaattg ttggatcttc tgatcaaagc
     4921 tctccggttg ccattgttca ttcaattgtt cacgaaaaac aaggctacat gtttttttat
     4981 gaaaattatt taaataccgg tcgtaataac caccgccaaa accgattcga taaccggctc
     5041 tattgtagat aatacctggt acaatgagga gatcgatcat ctccggcaaa tagagcttct
     5101 ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag aacggactgt tcatccattt
     5161 cataaaacgc catctgacgt ttcggcaaag ttttaggtac aactattttc ttttgttctt
     5221 tttctgctcg ttggataacc ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc
     5281 ctatcacttt tgctgacttc cagtactcgc tggcgaatag ctgtttatag attcgctgtt
     5341 ctttttcctt tttcttgcca cttttggcca gatttttcag cttttcgatt cctttccggc
     5401 gtaatcttac tttcaattgg tccaaaaact cacccttcct tctctcataa aatggtatag
     5461 gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc ataaatagaa taaaaagaag
     5521 acctagaaat gattggcctt ccttttttaa tgaatttcaa ctgttcagcg cgtgtttttg
     5581 tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt
     5641 aggtacttcc ccacttggta gaatcactct agcaaccata tctgcactaa gcagaaaaat
     5701 ggaaccgaac aaaccgctca gaacaaaggt ccagttattt tctttacgta cgactagctt
     5761 cgcagcatga ggagctagta gtcctacaaa agataaacta cctgtcaccg ctacactacc
     5821 gcttgccaaa gcaacagcta acaacaaaag gattttttga cttttagcca cgttaaggcc
     5881 taatccttgc gcgcgttctt gaccaaatga tagcaataca atcctctgtg cataaagtcc
     5941 tcctagcgga attaagataa ccgcccatgg aagcaatgcc caaacataag gccagctcgc
     6001 accccagatt gatcccgcta accatgcatt cacaaatcca taattttcct tagataattg
     6061 gatcgtgcat agcaatgtta atgcagaaag gccggcatta acggcaatac cggacaataa
     6121 gattcgattc atacccaaaa agccatttcg atcataggcc gcaagataga cacagcaagc
     6181 agctaaaaca ccacctagaa aggctacaac tagcaataga aacggcagcg aatagtgcga
     6241 aaagaaacct aaataaagca tcactaaaaa tcctgctccg gcattaatgc ccaatatact
     6301 agaatctgcc aattcatttc ttgttactcc ttgcaagata taaccagaaa cgccaagaca
     6361 agctcctgct aataaactga tcagtaatcg aggaactcga aattctctaa caatcagcgt
     6421 caaagaagat gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg
     6481 cgtaccaatc attaaactac ctatagacag gatgacaagt acaaaaagca agaaatagaa
     6541 cgtccaatat atttttttca ccgttgttcc ctccttactt gtatcagtaa aaacggaacg
     6601 ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag ggggattgat catcctcgct
     6661 gtaaaatccg ccaacataac caatccaccg ccaactaaaa aagagacagg tagaacataa
     6721 cgatagtccg ttccaaccat tccgcgaaca actggcggaa ccaacagccc aataaatgaa
     6781 acaggtccga ctaacgaaac ggcaatggct gctaacactg caactagaca gaaaacaatc
     6841 aaccgcgttc gtttaggatg acttccaaga gctaatgcat gtgtttcact caaatgaatc
     6901 aaagatattg cttttcctaa tgaaaaagaa accaaaacag caagtatata taaaggaaac
     6961 aatactgcta attgtttcca tgtgatattg gcagctccgc ctacaaacca aaaagcaaga
     7021 tcctgctgca gattaaagaa ttgggtaagt gcttggctga tagcagtaaa aaaagaacta
     7081 atagctacgc cagctaaaat cagctgtaaa ggattcatgc ccattttcga aaactgcaaa
     7141 aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca atgacattag aaacacagaa
     7201 aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg
     7261 ttgattccta gtaagcctga atctgctaaa ggatttcttg tcaccccttg catcaatgca
     7321 ccgctcaatg caaaacaact ccctacaaga aatgctcccg ccacacgagg caaacgaata
     7381 tttcgtatga tttggtgact ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa
     7441 tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga
     7501 acaaaacaga ccaaaagcag gagtctattt ttcttcatag tcctcataat tgatcctcct
     7561 gctcttttgg caacaaatca taggttagaa aaactggttt atttgttctt ggaagcgtga
     7621 caatcgttgc atcaatctca aatatttttt tcatacactc ttttgtgatc acttcttcag
     7681 gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa taaataatct gaaaaacgtg
     7741 aagcatgatt aatgtcatga atcgacatca agatcgttgt ttgtttcatt tgattgattt
     7801 cagtcaataa ctgtaggatc tctaattggt gtgccggatc taaaaatgtt gtcggttcat
     7861 ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc gatccagaca cgctgggttt
     7921 gtccaccaga cagcgttgcg atatcccgca tacgaaatcc agctagacct gtagcatcca
     7981 acgcccactc aattgcttca tgatcttgcg tgtccatgcc actgaacagt ttttgataag
     8041 ggtatcgtcc gtaagatact acttcttcta ctgtcatacc tgtagggtgt tcactagttt
     8101 gtgctaataa tgcaattttt tttgcaagtt cttttgaact gtatgtagag atatcagagt
     8161 tattgacaat gatctttcct gatttcacag gtagtatacg ggtaaaactt ttcaataaag
     8221 tagattttcc acttccattt ggcccaatca aactagtgat tttcccttca ggaatcgtaa
     8281 cagacatgtt ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca gctgctctta
     8341 atctcgtcat ttcacccatc ctttcgctga aaatcattct caattatgac gctgtttcaa
     8401 catttcgtca agcgctactt tcatatctat agccaaatga agcggttgac agcgttcgaa
     8461 atatcaggaa taataaccaa tgagaaagat tctcaattat gttaggagga aagaaatgaa
     8521 attaaaaaca aaagtattca cattatctag catattgctg ctggctggag tactgagcgc
     8581 ttgctccccc tcttcagata aagaaacagc tacaactgcc aattcgacga cttcatccaa
     8641 tagtactcat acagtcacag atacacttgg acataaagta gagattccaa acgcgcccaa
     8701 acggatcatc ggcagttact tagaagacta tctgattgca ctaggggaaa aacctatcgc
     8761 ccagtggact gttggcagtg gttccattca agattatctg caagatgatt taaaggatgt
     8821 cccaacgatt tcttatgacc ttccttatga gaaagtgtta agctttgagc ctgatttgct
     8881 gttgatttct tcttccgcta ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc
     8941 gccaacttat gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac taaaagatgt
     9001 cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc
     9061 catcaagaaa actcgagaag aactagcaga taaaatcgag aacaagacag ctgcagtgtt
     9121 gtgggtgacc aacaactcag cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt
     9181 atacgacgat ctggaatttg gtgttcctga tctagtggaa gaggtctcca aaaaagccac
     9241 ttctgactgg tctgctgttt cctctgaaaa gctagctgaa ttagatgctg actacatcat
     9301 ccttgtgaac agtgacaaag atgctgccat gttcaatgaa ccaagctgga agaatctaaa
     9361 agcagtgaca gacaatcatg tactgcaatt cggacctgaa tctagttggc tatataacgg
     9421 acctattgcc agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt gattttaact
     9481 aaaaaaagca tcttcacgat tagtcgtgag atgctttttt tcttattaac aaactagacg
     9541 catagatttt attcttcttt tgaagcaacc ctatttttta ctctttccca tttttgaaaa
     9601 cgatgggcat atagattttc ttgatccgtt atcccctcat actcctctat aagggcgaag
     9661 tctgaaaaat caatattccc aatatacgta tcgccttcaa acgtgctatc gatcaatgtt
     9721 cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa tccgactacc acccgaaaca
     9781 tagatatctt ctgagatcgt ttgactatac gacaaaagtt catcgatcga atgcattact
     9841 tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat atggcgataa
     9901 ggaagtgaaa gatcccccat tccttcatag gtctttcttc ccatcactaa aattcgatcc
     9961 attgtatgtt cgcgaaagaa acgcatatca tttggcaatc gccatggtaa caaaccattt
    10021 ttgccgatca gaccattttt atcttgtgcc cacatggaaa taaacatatt atccgctcct
    10081 tatgatttta gatgttttga gataaaaaaa gtctcaattc tttgaccagc aaggaattga
    10141 gaactttaaa aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa
    10201 tatttttgtt tttccaaacg atcaggattg ttacgtttgt ttttgcttgt tagatagtta
    10261 cgttctttac aagaagtaca ctctaaagtg atgtttacgc gcatgttttt ccctcccatt
    10321 tttacaagaa tcctctaaga tgactctcag acttgattat cttaccatga ttttttctag
    10381 attgctagtg tttttcacga ttttgttaag taaaactact tgacgataaa tacaacggtt
    10441 ttacttctca aacaaaatct ttttcttttt gtataaatac ccttacactt gtttatatag
    10501 attatgtttt ttaataaagg gggatactaa tatgccatgg aacatgaagg actttcctgc
    10561 atcaatgaaa aacttagata aattgacaag aaaaaaagca atcgacatcg caaatgcatt
    10621 attagatgaa gggtaccctg ataaccgtgc gatcccaatt gccattgatc aagcaaaaaa
    10681 atggtatgaa gatgctagtg aatcagaaaa acgaactttc gaaaaagaaa aaaatccaag
    10741 caagacggat gaacacgata cgaatcctcg tgctggaaaa ctgcttgatt cagatgtgat
    10801 cgtagaatat gaggaagaac aatggatcgt gaaatctaaa ggggcaaaaa aggcaagcaa
    10861 ccattttgat acaaaaaaag aagcaatcga aaaaggaaag caagtagcac aaaacaaaga
    10921 atcaactttg ttcatttata aaaaagatgg gacaaaagaa aaagaaatcg attattgaac
    10981 atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc cttatgttcg aaacaatggt
    11041 gttcctctgc caactccgaa ccataagtca taatatctat aaaacgggaa gagctgtttt
    11101 ttgaaattgt ttcttatcac tcagtgttaa acggtaggag aaaacctctt ttttattgat
    11161 atttgatttt catcaaacag tcacatagtc gattgcttct gcttgcggaa tcgtttcatc
    11221 tacaaagtag actttatacc atgtcaagaa actgaagatt gtccagattt ttctttgctc
    11281 ttctttacgt ccttcgtgat ggtcatctag caacttcatg attttttctt gatcaaaaaa
    11341 ttcttttgca aaatctgctt cgaataatgc tttgacttgt tcgtagccgt gttcttcttt
    11401 caaccaagct ttgattggca cagggaatcc taatttcaca cgattcgacc attcttccgg
    11461 aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg ttgttttgat tcaataaaaa
    11521 cttacttggt atttgttgag ccaatttcat gacttcgata tccaataaag gtactcggac
    11581 ttccaaagaa cttgccatag aaagtttatc tgcctttaac aagatatctt ttggcatcca
    11641 ttgatgaaga tcgacatatt gcatcttatt gacttcatct ttgattcctt ctgttttctc
    11701 gtaatgaaca gtcatgattt catccacaga tggtgctgtt tgatattttg gtgttaacac
    11761 ttctgatgct tctgattctt cgaacactcg agcatgaccg ataaaatatt cttttgcagg
    11821 agcaagtgat tcatataaat ggatccgtcc atggaagttt ttcatttttc cgattttacg
    11881 tcctaaatta tagcgtgttc cttttggtaa ttttttcaat ccttgagcaa tcacacggat
    11941 gaattttgaa ttcgtatgga atccatacgt ttggtatccc gcaaacagtt catccgcccc
    12001 ttctccagat tggactaccc gcacactttg tgaagcaaga tttgctaaaa agtacaaagg
    12061 tacgcaagaa ggatttgaat caggttcatc taagtgatac tggatcaaag ggaatgcttt
    12121 gaacgacatg tctccgtcga tcactgctgc tgtattatct aagtccaata gttcggttag
    12181 ttttctggct tcaatcgctt cattataggt tttatcatca aacccgattg agaaagaatg
    12241 atctggtttc aatacagagg ttacataact ggaatcaacc ccgctggata agaaagaacc
    12301 aacttctaca tcactgaccg tatgtgcttc aatagaagct tgaacggttt cgtcgatttt
    12361 ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca aaatctgcat cccaatattg
    12421 ctgaatgtct atttttccat ctttatacgt ataataatgg ccttcaggca aacgatagac
    12481 atctttgaag aatgtttcgc cgtttaatgg agaatattgg aaagtcatat aaggtttcaa
    12541 cgcttctttg ttcaattctt tgttaaaatc aggatgaggc agaaaacttt tgatttctga
    12601 accaaacata aatgttccgt tcatttctgc ataataatat ggtttgatcc caaagtggtc
    12661 tcttgcaccg aataactcat tcttttcatt atcccaaata gcaaaagcaa acattccgcg
    12721 gatcttttgc aataattctg ttccccattc ttcgtaacca tgcaaaagga cttctgtatc
    12781 tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct tctctcaaag gctggtaatt
    12841 atagatttcg ccattaaaaa taatgatctt tgtccgatct tcattgtaaa taggctgagt
    12901 accgccctct aagtcaataa tacttaatct tctgaatcct aaagcaacat gtttatcgat
    12961 atattctcct gaactattag gtccacggtg aacgattcga tccatcattg tattaataat
    13021 tgtttttttg ttgtccttgt cgttaacaaa acctacaata ccgcacattt aactttcaca
    13081 tcctaatata aaattactgt ttatttcatt catatgcatc ttatcataga ataaatagcg
    13141 gacgaatatt ttctctctta caatttcgtc acgtgaaaac aaaagatgaa agtttctgtt
    13201 tttacagaaa ataaagtccc aatatctaat ttaccatgtt ttcttttttg aggctacctt
    13261 attctcttcg tgttaaaaaa atattagtgg atgctatttc caaaaaaacc agcaaaaaaa
    13321 gacacgacct aaagatcgtg tc
//
LOCUS       DARBER010000066        13203 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARBER010000066 DARBER010000000
VERSION     DARBER010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.067"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..299
                     /locus_tag="SCK06_001764"
     CDS             <1..299
                     /locus_tag="SCK06_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151762.1"
                     /translation="DYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPV
                     RTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(396..830)
                     /locus_tag="SCK06_001765"
     CDS             complement(396..830)
                     /locus_tag="SCK06_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151763.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1062..1772)
                     /locus_tag="SCK06_001766"
     CDS             complement(1062..1772)
                     /locus_tag="SCK06_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1151764.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1762..2622)
                     /locus_tag="SCK06_001767"
     CDS             complement(1762..2622)
                     /locus_tag="SCK06_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG1151765.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2627..3265)
                     /locus_tag="SCK06_001768"
     CDS             complement(2627..3265)
                     /locus_tag="SCK06_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG1151766.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3280..3579)
                     /locus_tag="SCK06_001769"
     CDS             complement(3280..3579)
                     /locus_tag="SCK06_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1151767.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3622..5106)
                     /locus_tag="SCK06_001770"
     CDS             complement(3622..5106)
                     /locus_tag="SCK06_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1151768.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5126..5587)
                     /locus_tag="SCK06_001771"
     CDS             complement(5126..5587)
                     /locus_tag="SCK06_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1151769.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="SCK06_001772"
     CDS             complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="SCK06_001772"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG1151770.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6890..7660
                     /locus_tag="SCK06_001773"
     CDS             6890..7660
                     /locus_tag="SCK06_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1151771.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7933..8760)
                     /locus_tag="SCK06_001774"
     CDS             complement(7933..8760)
                     /locus_tag="SCK06_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1151772.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9328..10563
                     /locus_tag="SCK06_001775"
     CDS             9328..10563
                     /locus_tag="SCK06_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1151773.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11562..11822)
                     /locus_tag="SCK06_001776"
     CDS             complement(11562..11822)
                     /locus_tag="SCK06_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1151774.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11849..13150)
                     /locus_tag="SCK06_001777"
     CDS             complement(11849..13150)
                     /locus_tag="SCK06_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1151775.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4182 a         2756 c         2079 g         4186 t
ORIGIN      
        1 tggattatac tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt
       61 tcatgtataa agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc
      121 gtataaagcc agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta
      181 ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata
      241 ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc
      301 tgagcattgg aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact
      361 aagctagaat tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc
      421 ttttttcttc tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat
      481 cctactgatt cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg
      541 actggtccat aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct
      601 tggcgctgat acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca
      661 gtgatccaac gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca
      721 gattgttcaa atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg
      781 ctcttatata attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc
      841 ctttcttata caattctccc tctaataata cagttctttg actatttaat ctatcacata
      901 taactctcct gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat
      961 actttttgat aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat
     1021 caagtgttat cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca
     1081 tactttccat gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata
     1141 ttggggttga tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg
     1201 ttttctactg ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt
     1261 actccaggga cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc
     1321 cctgtatgaa cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga
     1381 accttaccat aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct
     1441 tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa
     1501 gtttgataca aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct
     1561 attatatttc ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg
     1621 acaccgcttg gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc
     1681 caagtcaatt tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc
     1741 ttcattgctg caattttttc ttcacaagac atcatagcca gcctccttca atttagaact
     1801 taagtagtct tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat
     1861 ttcaatcaga aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa
     1921 atcaacacaa ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac
     1981 agggtaagta tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg
     2041 agccggatta ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa
     2101 aggtgaatga atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc
     2161 atccatgatt tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt
     2221 tattccttcg ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta
     2281 tccagctatt tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat
     2341 tagttgtttc gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg
     2401 cgcacttaaa caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc
     2461 agctatcttt tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga
     2521 catctcgata aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat
     2581 atctttgggt aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac
     2641 gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac
     2701 cacgaccagt gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat
     2761 ttaatccacc agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata
     2821 aatctttttc accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta
     2881 cctgccggat accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga
     2941 gttctacttg caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac
     3001 aacaaatgac tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc
     3061 cagcgtctgc acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac
     3121 agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta
     3181 ctacatcacc aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg
     3241 caatttgcaa attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca
     3301 ctttgtttca gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat
     3361 aactgtaact ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa
     3421 aactacatcg tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt
     3481 ggtaattggt acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct
     3541 tgttcctgat ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt
     3601 gttgtctaat cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc
     3661 tacaatcaaa aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa
     3721 accaatgaca actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa
     3781 tggccaagct gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat
     3841 ccatgtagcg aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag
     3901 gcaagcggct ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa
     3961 gaacaacgga atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc
     4021 aatcatcaaa aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga
     4081 accaaagcca aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt
     4141 tgagattcct tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa
     4201 gatcgccaga tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg
     4261 cccttcttgc atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat
     4321 cccaaagaaa aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa
     4381 gaatcctggt aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt
     4441 ttcatcccgc tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg
     4501 ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa
     4561 gttagaagct actgtccaat aacaacctaa aatcagtccc ataaaaatca atgcttgtat
     4621 cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac
     4681 ctgaacgttt ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa
     4741 taagatattt aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc
     4801 aaatgtctcc attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat
     4861 ggcatccaaa ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa
     4921 accaccagag ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat
     4981 gcattcatga agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag
     5041 gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc
     5101 ccccatcttt tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat
     5161 cgatggagtt cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata
     5221 atttttggat attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac
     5281 tagccgtttt ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg
     5341 taaatgaaat agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta
     5401 cttgaggtgc gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca
     5461 catacgtttc atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt
     5521 cacgccaatc cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt
     5581 tcagcaagaa actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat
     5641 cgagaagaca tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg
     5701 atagatgaat ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc
     5761 ctttttgaat ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat
     5821 atcgtagtgg acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc
     5881 aacagaagta actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac
     5941 atcgattttg tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc
     6001 atgataaaga ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc
     6061 ctgaggcatt tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg
     6121 atcaaacgct tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt
     6181 tactgtaatc gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt
     6241 tggcccaatg aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc
     6301 tagatgatcg gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg
     6361 atcgattacg aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg
     6421 catcatctga tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca
     6481 ccatagatca cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc
     6541 cagccaccac atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa
     6601 gtatgtgccc cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac
     6661 agttttactc atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa
     6721 tgtgtatcag catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct
     6781 ttcagtaatc aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa
     6841 ctagtcaaat aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc
     6901 acttgtcaat attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca
     6961 actatcaact ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga
     7021 tttaaattta ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa
     7081 ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc
     7141 cagagaagct agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac
     7201 tgcgttgaaa acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt
     7261 gaaaatcaat tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat
     7321 gcgctttcca aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa
     7381 tgcagacatt tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa
     7441 gatattaaat gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat
     7501 tttagtcgct gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc
     7561 agtctttgat tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag
     7621 aaatacgggg ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt
     7681 aaataggatt atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc
     7741 tcaaattgat aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg
     7801 cttcttttat aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag
     7861 gtatgacaaa agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat
     7921 ttcacaacca ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt
     7981 gaaacgcgct attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg
     8041 cttatggcaa gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt
     8101 cttattatac tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt
     8161 acggtaatag gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt
     8221 ctcactcaat attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt
     8281 tgacaagtag atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt
     8341 cgtttgttcc aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag
     8401 atgatcaagc agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt
     8461 ttcctgatcg atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa
     8521 tgattgaatc tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc
     8581 ggagatctcg acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat
     8641 attcttgttt tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac
     8701 ttgtgttaac ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa
     8761 aacaatttcc ttccctttaa aaaattattt ctcctttata tcatacacaa aaaaaagttc
     8821 taaacaacca aaaatgttta gaacagaata aaaattttaa ttttaaaata atattcaaat
     8881 taaacaactg gcgtatctgc tccttgattt ctttttgtgt tatctgaata attatcgttt
     8941 acagttataa cacatagtga aaattgtcat ggcgagaaag tctaagaata tttttaaaca
     9001 taactatttc tttcttacgt gtgtataatg acgacagcac acctatcaat taatataatt
     9061 attttatttt tttatgttct catgtttatt tttaatgaca caacttctcg tatttatctg
     9121 gcacaacata taaatcgatc aaaaaattgt tgcgtgtttt tttgaaaatt ggagtttcaa
     9181 cttgattaca aaatttaaat ttagaagttg tgaaaacagc gagagacagt ttaatattca
     9241 aaatatacat aggctactcg aacgctgtgt tttttagtat actagtaaga aacgaaggga
     9301 tagactcatt ccaataagga ggatttcatg aagacaattc tttttgctcc tgcagtattc
     9361 aatttagctg agacaacacg aatgattgag gttgccaaag ctatgaaaga ccgttattac
     9421 tgtgtattct ttggcttttc taaaactttt gctcctttga tcacagaaag tggctttgag
     9481 tatcatttac tagcgccgat ccttacgaaa aaacaagaag aacaaatcat aaaatttgac
     9541 cagttgcgta cacttaaaaa cccttttacc aaagaaatgg ttgcctctcg tatcactagt
     9601 gaacagagtc tgattgctaa gtttgagcct cgactgattg tgacaggttc gaatgtgaca
     9661 atttttttat cggcccgcaa taaaaagatt cctttagtat atgttaaacc ctatgctttg
     9721 agtgaacctt tttttcaatc agagcatgcg ctgatgcccc acgagttacg tttcttcggc
     9781 cctttaagca atcccctttg gcactggaca aaaaagcgta tagtaaatac ccgatggatc
     9841 cccaatgcct tcaaacacgc tgcacaaaaa gaaatgcttc ctttaccaaa aacaagttta
     9901 caaatgatgg atgctgatct aaatctcatc acaacgccta aactattttt ggaagaagaa
     9961 cagctgccgg aaaattatcg aatcgttggt ccaatttttg ctaagctcag tacttccttg
    10021 cctgaagaag tgctaacttt tatacagcga aagcaacaag aaagaaaacg tattatctat
    10081 ttcgcaatgg gtagttcagg caatccgcgt atgttacgca aaattttgtt cttcttacaa
    10141 aaacaaccgg atctagcggt tattgcacct attgactatc tgcttgatga cattcaatct
    10201 gactcagaag atcttttaat aacagaatat ctgtctagtc atcttttgca tgaattcatt
    10261 gatttttctt ttatccacgg cggggaaggg accgtccaaa ctgcctgtga atccggcaaa
    10321 ccgtttatcg gtgtggggat gcaatttgag caaagctgta atattgacta ttgtgtaaaa
    10381 tacggaaacg caatcgccct cacaaaacca gtgactgaaa gaaaactgca gattgctctg
    10441 cgcgaagttt cttcacccaa gattcgtcag caggcacgca cgctgcaagc cgtttttcca
    10501 ataaatggtc cacaacaagc aattcaagaa atcgaaacct tcctgagcaa ccacccaata
    10561 tagtttcaag tagaaaaagc ttttttaata gagactcatc agtaatttcg ctattcgaga
    10621 cccagacagt gaaaatacaa aaaaagtaaa tagtggattg aaaagttaga gttacttaaa
    10681 ttacggtgtc taaaaaacac aaaatcatcc ttaaaataaa agatctgacg ctatccaaat
    10741 gagcataggt agcgtctgcc atttattctt agtcaaattc tttgacacct ttctctggtt
    10801 ggatcatctc aacgtttctt caaaagaaag accataataa ttaggcaaat acattttctt
    10861 aaaatgtgtc atatagatct agaagcatcg ctttgtagag atttgtgccg ctagtcccct
    10921 caacaattac taaatcaaat gtaggattta ccactttatc tttgataatc aacctccctc
    10981 ttctgttgag acaatgatct cgcaatccga tgcatactcc tatataaact gccagcaatc
    11041 cccacatatt gagtagatca tgtcagaatg ttctatgtcg tcagcaacga tacactaaaa
    11101 gtcacgttca ccatgactga ttgctattcc cagcacaatt atttctatac acgtttcatg
    11161 atttgaataa acactcacat catacatatt accattcata caccatactg cagcccctac
    11221 tatatggttg taagctccca aatctatatt tgacttgatc actttggtag tagatctact
    11281 aattctaaat ccattgctga taaaatttcc attttttcct ccttctttct gccaaaacag
    11341 ttaaaacaat aataatcttt atgtaaaaaa gggctgtgac aaaagccttg tcacagtccc
    11401 atattccgaa taaacggtgg tcaaaagctt gcatctgcgg taataagttc aaccaaacga
    11461 aaaataagcc aacatttcac aaaaatttaa gaagcaattt tcgtaaattt cttcttattt
    11521 cttgaagcta accacttctg ttacaaccac ttttagaaca tttaaagctt cgatgtgaca
    11581 aaccatctat gctgctcgat cataaaattg atagcaaaaa ttccacaaag tacatatgtc
    11641 ccccatgaaa acaaaacatt catatgcggt acaatgatct ttggaagaat cacgatcaaa
    11701 aatgatgcca gtgcccaaat ccatcgattg atttgaatat gatatgtagc tagcatattc
    11761 aatataaggt gactagccgt taataaaaag ataaaaacaa aaaataaaat cagccaatac
    11821 attgtttccc accttctaaa aaccaaactt agacagtctg gacttcgttt tctccacgac
    11881 tttgttcatc tgacagcatc tttttatcat aagcttttac aaatggataa tagataataa
    11941 atgctatcaa tgtgttgatc atgatcaaga taacaacacg ccagtcattt cctgctgaaa
    12001 gataaccgcc gattggagct ggtaatgtcc atgttggcaa aacagtaaat ctattgacca
    12061 tttctaattt gactgcaaaa taactgataa tagtcaaaac taaaggaact aagttgaacg
    12121 ggatgaacat gtaaggattc aacatcatag gagctccaaa aattatgggc tcattgatat
    12181 tgaagatacc aggaataata gaaaaacgac caacctcttt caagaatttt gatttacaga
    12241 agaatataaa caaaacagat accacgatcg ttgctcctgc accaccaatt gttaccgtcc
    12301 attgataaaa ctgttcagga gcgatatagg gtaattgggt ggcagctgta ccatctgcca
    12361 atgcctttgc attctcatcc aacatgatca gccacattgg acgaacaaca gcccctacaa
    12421 tactcatgcc atggatacca aatgaccaca acaaatggat caaaaacatt ggtagtaaaa
    12481 caccaagcaa attattccct aaaatattgg ttaaaggctc gaataacgat aataaggcag
    12541 cgtttacatc aaagcccgcg acgcttcgaa tcagccaagt aattgttaaa atgactgcac
    12601 ctggaatcaa tgcctcaaaa ctacgggcaa cagcaggtgg cacttgttct ggcattttga
    12661 tagtaatatt tcgattttta aagaaacgat atacttccac tgcaaaaatc atagataaga
    12721 tccccgcaaa catcccagat gctcccaaag aactcatagg taagacccag ccaagaggtg
    12781 cctcttcagg agttaaaacg tttacaggaa cttgtaacat aaagaacgtt cctaaagata
    12841 atataccacc tgtcacacta tctaatccat atgatttagc tagatccgaa cccataacaa
    12901 aagttgcata aatagccatc aatcccatcg tcatcctaaa gggtaaaaca atcgttgccg
    12961 aataaggggc aataaattca ttccatccag gaaacggtaa attgataaag accataaaaa
    13021 atgtcccaat caaaatcaat gacaatgttg aaacaacccc attacgaatt gctaataaat
    13081 gacgctgtgt accaattttt gccattggtg ggacaagttt ctcttcgacc catttcatca
    13141 gcttttccac taaaaccact ccttttcaat aaaatatgaa ccattcattg catgacaacg
    13201 caa
//
LOCUS       DARBER010000067        13149 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARBER010000067 DARBER010000000
VERSION     DARBER010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.068"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(79..151)
                     /locus_tag="SCK06_001778"
     tRNA            complement(79..151)
                     /locus_tag="SCK06_001778"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="SCK06_001779"
     CDS             532..1101
                     /locus_tag="SCK06_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG1151776.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="SCK06_001780"
     CDS             1088..1948
                     /locus_tag="SCK06_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1151777.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /locus_tag="SCK06_001781"
     CDS             2138..2842
                     /locus_tag="SCK06_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1151778.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="SCK06_001782"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="SCK06_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG1151779.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /locus_tag="SCK06_001783"
     CDS             4679..5995
                     /locus_tag="SCK06_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151780.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="SCK06_001784"
     CDS             5996..6865
                     /locus_tag="SCK06_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG1151781.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="SCK06_001785"
     CDS             6920..7729
                     /locus_tag="SCK06_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1151782.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="SCK06_001786"
     CDS             complement(7832..8461)
                     /locus_tag="SCK06_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG1151783.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="SCK06_001787"
     CDS             8631..9923
                     /locus_tag="SCK06_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1151784.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="SCK06_001788"
     CDS             complement(9999..10661)
                     /locus_tag="SCK06_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1151785.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /gene="groES"
                     /locus_tag="SCK06_001789"
     CDS             10886..11170
                     /gene="groES"
                     /locus_tag="SCK06_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG1151786.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="SCK06_001790"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="SCK06_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG1151787.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4372 a         2265 c         2833 g         3679 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    12841 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
    12901 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
    12961 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
    13021 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttgtgtg
    13081 taattataat gaaaatagtt atttttaata gttataatat tttatctatt aaaagtaaat
    13141 atccattca
//
LOCUS       DARBER010000068        13149 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARBER010000068 DARBER010000000
VERSION     DARBER010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.069"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            38..1777
                     /gene="yfcC"
                     /locus_tag="SCK06_001791"
     CDS             38..1777
                     /gene="yfcC"
                     /locus_tag="SCK06_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1151788.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1934..3118
                     /gene="iadA"
                     /locus_tag="SCK06_001792"
     CDS             1934..3118
                     /gene="iadA"
                     /locus_tag="SCK06_001792"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG1151789.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3229..3432
                     /locus_tag="SCK06_001793"
     CDS             3229..3432
                     /locus_tag="SCK06_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1151790.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3533..4195)
                     /locus_tag="SCK06_001794"
     CDS             complement(3533..4195)
                     /locus_tag="SCK06_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151791.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4208..5710)
                     /locus_tag="SCK06_001795"
     CDS             complement(4208..5710)
                     /locus_tag="SCK06_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151792.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6258..7937
                     /locus_tag="SCK06_001796"
     CDS             6258..7937
                     /locus_tag="SCK06_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1151793.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8064..8543
                     /locus_tag="SCK06_001797"
     CDS             8064..8543
                     /locus_tag="SCK06_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG1151794.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8762..9442
                     /locus_tag="SCK06_001798"
     CDS             8762..9442
                     /locus_tag="SCK06_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG1151795.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9450..10781
                     /locus_tag="SCK06_001799"
     CDS             9450..10781
                     /locus_tag="SCK06_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1151796.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10796..11758
                     /locus_tag="SCK06_001800"
     CDS             10796..11758
                     /locus_tag="SCK06_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG1151797.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11869..12207
                     /locus_tag="SCK06_001801"
     CDS             11869..12207
                     /locus_tag="SCK06_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG1151798.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12286..12993
                     /locus_tag="SCK06_001802"
     CDS             12286..12993
                     /locus_tag="SCK06_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG1151799.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4176 a         2328 c         2895 g         3750 t
ORIGIN      
        1 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
       61 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      121 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      181 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      241 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      301 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      361 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      421 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      481 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      541 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      601 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      661 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      721 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      781 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      841 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      901 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      961 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1021 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1081 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1141 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1201 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1261 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1321 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1381 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1441 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1501 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1561 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1621 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1681 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1741 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1801 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1861 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1921 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1981 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2041 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2101 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2161 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2221 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2281 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2341 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2401 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2461 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2521 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2581 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2641 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2701 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2761 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2821 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2881 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2941 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3001 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3061 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3121 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3181 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3241 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3301 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3361 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3421 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3481 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3541 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3601 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3661 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3721 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3781 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3841 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     3901 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     3961 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4021 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4081 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4141 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4201 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4261 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4321 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4381 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4441 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4501 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4561 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4621 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4681 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4741 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4801 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4861 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     4921 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     4981 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5041 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5101 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5161 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5221 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5281 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5341 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5401 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5461 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5521 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5581 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5641 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5701 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5761 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5821 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     5881 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     5941 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6001 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6061 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6121 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6181 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6241 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6301 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6361 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6421 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6481 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6541 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6601 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6661 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6721 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6781 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6841 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     6901 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     6961 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7021 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7081 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7141 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7201 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7261 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7321 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7381 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7441 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7501 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7561 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7621 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7681 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7741 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7801 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7861 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     7921 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     7981 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8041 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8101 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8161 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8221 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8281 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8341 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8401 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8461 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8521 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8581 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8641 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8701 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8761 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8821 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     8881 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     8941 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9001 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9061 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9121 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9181 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9241 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9301 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9361 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9421 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9481 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9541 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9601 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9661 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9721 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9781 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9841 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
     9901 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
     9961 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10021 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10081 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10141 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10201 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10261 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10321 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10381 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10441 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10501 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10561 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10621 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10681 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10741 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10801 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10861 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    10921 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    10981 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11041 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11101 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11161 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11221 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11281 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11341 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11401 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11461 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11521 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11581 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11641 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11701 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11761 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11821 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    11881 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    11941 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12001 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12061 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12121 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12181 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12241 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12301 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12361 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12421 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12481 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12541 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12601 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12661 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12721 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12781 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12841 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    12901 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    12961 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13021 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagggctc
    13081 tttgtcaata aggactgatg ggttgtacaa aatcaaatct gggtcagaat gaacctcatt
    13141 ctggctcag
//
LOCUS       DARBER010000069        13137 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARBER010000069 DARBER010000000
VERSION     DARBER010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.070"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..712
                     /locus_tag="SCK06_001803"
     CDS             <1..712
                     /locus_tag="SCK06_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1151800.1"
                     /translation="KSSSNYYEGGNEIVSKMKISEVTLDETDDFTYYKGYKKGWNTLC
                     TYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQL
                     KISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLY
                     SRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKL
                     IIGISSTVTDYISGYLDK"
     gene            779..1273
                     /locus_tag="SCK06_001804"
     CDS             779..1273
                     /locus_tag="SCK06_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1151801.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1329..1967
                     /locus_tag="SCK06_001805"
     CDS             1329..1967
                     /locus_tag="SCK06_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151802.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2039..2434
                     /locus_tag="SCK06_001806"
     CDS             2039..2434
                     /locus_tag="SCK06_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1151803.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2418..4871
                     /locus_tag="SCK06_001807"
     CDS             2418..4871
                     /locus_tag="SCK06_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1151804.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4868..6895
                     /locus_tag="SCK06_001808"
     CDS             4868..6895
                     /locus_tag="SCK06_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151805.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6892..7914
                     /locus_tag="SCK06_001809"
     CDS             6892..7914
                     /locus_tag="SCK06_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1151806.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7931..8860
                     /locus_tag="SCK06_001810"
     CDS             7931..8860
                     /locus_tag="SCK06_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1151807.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            9105..9221
                     /locus_tag="SCK06_001811"
     CDS             9105..9221
                     /locus_tag="SCK06_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1151808.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9237..10040
                     /locus_tag="SCK06_001812"
     CDS             9237..10040
                     /locus_tag="SCK06_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1151809.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            10275..10511
                     /locus_tag="SCK06_001813"
     CDS             10275..10511
                     /locus_tag="SCK06_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151810.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10518..12470
                     /locus_tag="SCK06_001814"
     CDS             10518..12470
                     /locus_tag="SCK06_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1151811.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12542..13039)
                     /gene="dfrG"
                     /locus_tag="SCK06_001815"
     CDS             complement(12542..13039)
                     /gene="dfrG"
                     /locus_tag="SCK06_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1151812.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         4523 a         2172 c         2546 g         3896 t
ORIGIN      
        1 aaagagctct tcaaattatt atgaaggagg taacgagatt gtttctaaaa tgaaaatatc
       61 agaggtaact ttagatgaga ctgacgattt tacttattat aaaggatata aaaagggctg
      121 gaatacgctt tgcacatatc ttaaaataag agttcctttt gatgatttag acttttttga
      181 aagtcataaa gatttgatta cccagacagc aagctccata tatgataaac aaggtgataa
      241 tgtattagtg gatactattt tagtaccact tccagagaat tacgaagtaa ttaactttag
      301 tcagttgaaa attagtgatg tggtttcaca agctattgaa gacgctgagt cttttatgag
      361 taatggtgaa tatcaacgtg cctttgacag agttcacact gcttttcatg gatatttaat
      421 tgaaatacta aaaaaatatg aaattacagt tcctagagac gaaaatctat ctaaattata
      481 tagtaggata caacaattaa ttgaaaaaga aattcaacct actgaacttg ctgatatagt
      541 gaagacaacg ataaggtcat caaatggaat gattagctct cttaatgaag caagaaatag
      601 gcattcactt gcacacccta atacaaatat tattggaaag agagaagcaa aactaataat
      661 aggtatttca agtacagtta cagattatat tagcggatac cttgataagt agcgtcatta
      721 actgattgct acttttttta tgactatttt tataaaaagg atgtgataag aattggaaat
      781 gaaagtctat gtggcaaatt taggacggta taatgaaggt gaattagtcg gagcatggtt
      841 tactccacca attgatgagg aagaaatggc agaaagaatc ggtttaaatg aggactacga
      901 agaatatgca atacatgatt ttgagcttcc ttttgatgtg gacgaataca cgccgattag
      961 tgaaatcaac cgtctatgtg aagccattca agaaatcgaa ggtacaccta tttataatga
     1021 actcaaagaa attcaaggca tgtggtttag tagcttggaa gaattactgg aaaacaaaga
     1081 ggatattcat tgttatagtg attgtgattc gatggaagat gtcgcccgtt attacgtgga
     1141 agaaacagga cagcttggtg aagtcccttc caatttacaa aattatattg actaccaatc
     1201 tttgggacgt gatatggaaa tagagggtaa ttacctagtt acctctcatg gggtatttga
     1261 atattgtcag taagtttaac aatccgtgtt ataattaaat taataaatag cacggagagg
     1321 tataaattat ggtaaatgta tttgaaaatc ttaaactatt aaaactagac atgaaagata
     1381 aaggctgggt gatagattcc ttttactttc gttacaaaca gcaaaattat attgtacttg
     1441 ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc tctgttaaaa ctagaatttt
     1501 taaaggaaaa cgatttttca gatatgctag ctgtctatgc taattcggta aaattatata
     1561 cagacacgaa aacaataaga gaatattttg gtatcgaata cagtagtaac ttgggagatg
     1621 ttttatttca atttagccaa actcttgctc gatttattcc gacagaagtt agtgagaaga
     1681 aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag tcaaagtgat tcagaagatc
     1741 ccagaaaaaa atattgtttt tcagttagaa gaaatccttt aaagggaaat ggtgagctgg
     1801 gaaaacgaag tccttttaat gacaataaaa cgagattgta tagacccagc ttatatgaac
     1861 gattagggtc agataccaat ttaagtttca gatattcaat gaatccagat gatgaagaaa
     1921 ctgatgaagg tattattgca aaatggacaa aaaataaaat tgaatagtta aagcattgaa
     1981 agagcaatca cctattttga ttgttctttt tctattttta cggaaaggac gtgaagacat
     2041 gaaaaaaata agaagttaca ccagtatttg gtcagtagaa aaagtgattt atgcgattaa
     2101 tgatttacaa ctccctttcc caattacctt tacgcaaatg acatggttta tcgtttcttt
     2161 gtttgtcgtc atactctttg gcagtttacc accactttct ctgattgacg gagcactact
     2221 caaatactta gggattccca ttgttctcac tgggttcatg tgccaaaaga cctttgacgg
     2281 aaagaaaccc tatggatttc taaagtctat cattacctat gctctacgcc ctaaagtaac
     2341 ctacgctggc aaacaaatac gattaaaaaa agaaacaatg aatgaatcga ttacgattgt
     2401 aaggagtgaa caactagatg gcatatccga ttaaatatat ggaagataat ctcgtattta
     2461 atcatgatgg cgaagtattc gcttattatg aattaattcc ctataattac agttttttaa
     2521 gttccgatga aaaaattcaa gttcatgata atttctgtca attgattgcc caaaatcgtg
     2581 atggcaagat tcatgctctg caactagcaa ccgaatcaag tattcgagcc acacaggaac
     2641 aatcgaaaaa agaaatcact ggacgattaa aagagatagc ttacgaaaaa atagatgaac
     2701 agaccgaagc gttaatttct atgattggtg acaatcaagt agactatcgc ttttttattg
     2761 gttttaaact ctacctcaat gaacaggaag tatccgtaaa aaatgttggg aaagaaattc
     2821 aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa attaatgggc gattttgttt
     2881 ctatgcctat ggcggaaatc gaacgctttt ctaagatgga acagttgtta gaaagtaaat
     2941 tatccagacg ctttcagatt cgtaaattgg agaaagatga ttttggttat attattgaac
     3001 atctttacgg gcaaacaggt gtcccttttg atgactacga ctaccattta ccgaaacgat
     3061 acgaagataa aaaagtgttg gtaaagaaat acgatattct caaacccact cgctgtttaa
     3121 tcgaagaaaa tcaacgctat ttacggattg aacaggaaga taccactact tatgtggcgt
     3181 attttacaat taatagtatt gttggtgaat tggatttccc taatagtgaa attttttatt
     3241 accagcaaca gcagtttact ttccccgttg ataccagtat gaatgttgaa attgtcgcca
     3301 ataaaaaagc cttatctacc gttcgcaata aaaagaaaga actgaaagat ttagatagcc
     3361 acgcatggga aagcgacaac gaaaccagta ctaatgtggt ggacgcttta gaatctgtcg
     3421 atgaattaga aagtaattta gaccaaacca aagaatctat gtacaaacta agctatgtgg
     3481 tacgggtatc agcaaagaat ttggacgagc tgaaacaacg ttgtgatgaa gtaaaagatt
     3541 tttatgatga tctgtctatc aaattagtac gaccttttgg cgatatgcta ggcttgcata
     3601 atgaatttat tccctcaagc aagcgatata tcaatgacta tattcaatat gtcacgtcgg
     3661 attttttggc tggtcttggt ttcggtgcta cacaaatgat tggtgaaaat tctggtattt
     3721 acgtcggtta caatctcgat acaggcagaa atatttatct ccaacctagc cttgcaagtc
     3781 aaggaattaa aggctctgta acaaatgcat tagcgtccgc ttttattggt tcattaggtg
     3841 gcggaaaatc attctctaac aatatgctgg tctattattc agtactcttt ggcggacagg
     3901 caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa agaaacctta cctgatattg
     3961 cccatgaaat caatattgtt aatctcacga gtgagaacga caataaaggg ttacttgacc
     4021 cttatgtgat tttaaaactc ccgaaagatt cagaaagcct tgccattgat attctcacgt
     4081 tcttaacagg tatttccagc cgagatagcg agaagtttcc tgtcctgcgt aaagctattc
     4141 ggagtgtaac tcaaagcaaa caacgagggt tgttattggt gattgatgaa ttgcgaaaag
     4201 aagatacgcc catcagcaat agtattgctg accatatcga aagctttgtc gattatgatt
     4261 ttgcccatct gttattcagt gatggcagtg tcacccaatc cattagcttg gaaaaacaac
     4321 tcaatattat tcaagtggca gatttggtct tacccgattc caatagctct tttgaagaat
     4381 acactaccat ggaattatta agtgtggcaa tgctgattgt cattagtacc tttgctttag
     4441 actttatcca ctctgaccgt tccattttta aaattgttga ccttgatgag gcttggagtt
     4501 ttttacaagt ggcacaagga aaagctctat ctatgcgatt ggttcgagca ggacgtgcca
     4561 tgaatgcagg ggtctatttc gttactcaaa acgcggacga tttactagat gaaaagatga
     4621 agaataatat tggcttgaaa tttgcctttc gttccactga cctagtggaa atcaaaaaga
     4681 ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa tcagaaacgc ttacgtgaat
     4741 tagaaaatgg acaatgttta gtttcagaca tctatggtcg tgtaggggtt atgcagtttc
     4801 acccgatttt tgaagagttg cttcatgcct ttgataccag accgcctgtg cgaaatgagg
     4861 tggaagaatg acacaaaaac aaaagaaatt tcttcactat agttggataa cgctcctttg
     4921 tgtgggcgtt attttacttt tattaggcac gctgggcaat gcagtacaag cgacaggctt
     4981 agttgatgaa accattgata caagcaatga atactccaag tatggtttga atcaatatca
     5041 gcttgattat tatgtggaca atagctgggg ctggcttcca tggaattgga gtgatggcat
     5101 aggacaatcg gtgatgtatg gattgtatgc cattactaat tttatttgga caatttctct
     5161 ttatctatcg aacgccacag gttatttggt gaaagaagcc tatgccttag atttcattag
     5221 tcaaacatcc caagcaattg gcgaaaacat gcagacgatt gcaggcatta caccgaatgg
     5281 cttttccagt tcagggtttt atgtcggttt cctccttctt tttattctca ttgttggaat
     5341 ctatgttgcc tatacaggat tggttaagcg agaaacgacg aaagtgatac gagctgtttt
     5401 aaactttgtc gtggtgttta ttctatcggc ttcctttatt gcgtatgcac ccgattatat
     5461 tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc agtctgaata ttgggacaaa
     5521 aattgttctt cctaacacgg atagtcaagg gaaagacagt gtggacatga tacgtgacag
     5581 cttattctct gttcaagtaa agcaaccatg gctattgctt caatatggct ccactgatgt
     5641 agaagcgtta ggggaagaac gtgtggaaag tttattgtct accagtccag atacaaacaa
     5701 tggtgaagat agagaaaatg tagtgattga agaaattgaa gatcatgaca atacctattt
     5761 aaccctaccg aagacgattt ccagattagg aacagtcttc tttttgtttg tattcaatat
     5821 tggtatctcg atttttgttt tccttctaac aggaatcatg attttttcgc aagtgctatt
     5881 tattattttc gctatgtttt tgccaatcag tttcctatta agtatgttgc cgagttttga
     5941 tggcatgggc aggcatgcca ttaccaagtt atttaatgtg attatgacaa gggctggtat
     6001 taccttgatt attaccgttg cctttagtat ttccactatg ctttattcgc tgtcagcaag
     6061 ttctcccttt ttcatgatta tgtttttaca aattgtgacc tttgcaggga tttattttaa
     6121 actgggcgat ttaatgagcc tattcacttt acaaagcaac gactctcaaa acatgggcag
     6181 acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa accagaagat ttcaacgcag
     6241 tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag caaaagacaa tgacaacaaa
     6301 gcagaatcaa accaataaag ctaaccatac tcgtcaaaat gaacggacta caccaaagaa
     6361 agaaacagca ctttccaatg tgaaaaagca acaaggcggt atcgggcaac gattaggtga
     6421 aaagacagcc catgtattgg acacgaaaaa tcgtctagtc aataaaagaa agcaggcaaa
     6481 acaacagata caggcgacac caacccatgt caaatatgcc cttcatcaag gaaaagagaa
     6541 tgtcactcat aatgtaacag actttaaaca atctatggca gataccaaag ctcaacgcaa
     6601 acaacagcgt atgaaccagc aggagcaaaa acgaaagaac attgctcaaa agcgtttgga
     6661 gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca tcatctcctg ttcagaataa
     6721 ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct gttttgtcac aaaacactaa
     6781 acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa caaaagacac caccgaagca
     6841 attcaatcag aggaagctaa ataaaagaac ggataagcga ggacaacaaa catgaagcta
     6901 aaaataacag tgatttgtag tgtggtcttc tctctcttct tttttctctt attattccta
     6961 gtcattttct ttgcagatga tacggacagt ggcgaaaaca acaaggattc ttctattcca
     7021 caaggcggtg tgaccgtttc accagaagtg ttggcacacc gtccgttgat tgaaaaatat
     7081 ggtaaggaat atgggattga agactatgtt tcttatatct tagccattat gcaagtggaa
     7141 tcgggcggta cagcagaaga tgttatgcaa agttccgaat ctttgggttt accgcccaac
     7201 agtttgagta ccgaagagtc catcaaacaa ggggttaaat atttcagtga attattaacc
     7261 agtgcagaac aacaaggagt ggatatagat tctgttatcc aatcttataa ttatggcggt
     7321 ggctttttaa attatgtgag gagtcatggg aaaaaatata cctatgaatt agccgaacag
     7381 ttttctaaag agaagtcggg tggtcaaaaa gcagactatc ccaatcctat agccatacct
     7441 gtgaatggtg gttggcgata taactatgga aatcagtttt atgttcagtt ggtttcacaa
     7501 tacctaaccg acacttcccc aacagaattt gatgatgaaa ccgtgcaagt cattatggac
     7561 gaagccttga aatacgaagg gtttccctat gtctttggag gggcgtcgcc taccacctct
     7621 tttgattgta gtggcttaat acaatgggtg tatgacaaag ctggcatttc tctccctcgt
     7681 gtcgcccaag atcagtatga cgctacgcaa gaaatctcta tggaagaagc ccaagcaggc
     7741 gaccttatct tctttcattc aacctataat gcaggcacat atgtgactca tgtggcgata
     7801 tatttagagg gaaatcgctt ttatcatgca ggcgacccaa tcggttatgg cgacttaagc
     7861 agtcgttatt ggcaagacca tctgattggg gctagacgtg ttattcataa ctaagaaagg
     7921 aatgaatcaa atgattataa aaatcgagcg aaaagaaaag aaggagaaac caaagaaatc
     7981 taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa gttggcacgc ataagaaatt
     8041 aacctttgtt ttatgggttt tattgattgg tagtgtcgga tttggaatct ataaaaattt
     8101 tactgccatt gacactcata ctgtccgtga aacagaaatc attaaacagc agattgtcga
     8161 taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa gactactttt cgtggcaaca
     8221 atcccaagaa gccattgata aacgaaatga aaaattgacc cattatttga cggaagaatt
     8281 gcaggtatta aatgaagaaa tgattcgtaa ggatattcct actagttcta gtgtgaacga
     8341 tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc tttgaggtac tgttttctgt
     8401 tgaacaagtt attaccgaag acaaagacaa ggaaactata tcgtctagct ttcatgtggt
     8461 tgtccatata gatgaatcag ataatatggt tattatcaaa aatccgacaa tgagtaagaa
     8521 acctcaaaaa tcggactacc aacccaaaca gctagaaagt gaccatacag tagatacaga
     8581 aacgatggac gaaatcacta gctttttaga aacgttcttc cagctctatc ctaccgccac
     8641 agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta cctatgatta acaaggaata
     8701 tgttttcgag gaattggtga atcctatctt taccagaaaa gataaccaag taatcgtgaa
     8761 tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc caaatctcgc aatttgagtt
     8821 gatattagaa aaacaagata attggaagat tgtaaaataa caaatattgg tacatgatta
     8881 cagatacttt gtaatcatgt actctttttg ataaaaaatt ggagattcct ttacaaatat
     8941 gctcttacgt gctattattt aagtgactat ttaaaaggag ttaataaata tgcggcaagg
     9001 tattcttaaa taaactgtca atttgatagc gggaacaaat aattagatgt ccttttttag
     9061 gagggcttag ttttttgtac ccagtttaag aataccttta tcatgtgatt ctaaagtatc
     9121 cagagaatat ctgtatgctt tgtataccta tggttatgca taaaaatccc agtgataaaa
     9181 gtatttatca ctgggatttt tatgcccttt tgggtttttg aatggaggaa aatcacatga
     9241 aaattattaa tattggagtt ttagctcatg ttgatgcagg aaaaactacc ttaacagaaa
     9301 gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa ggtacaacga
     9361 ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca ggaataacct
     9421 cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat atggatttct
     9481 tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg atttctgcaa
     9541 aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa atggggattc
     9601 ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca acggtttatc
     9661 aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta gaactgtatc
     9721 ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta atagagggaa
     9781 acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg gaactcgaac
     9841 aagaggaaag cataagattt cagaattgtt ctctgttccc tctttatcat ggaagtgcaa
     9901 aaagtaatat agggattgat aaccttatag aagttattac taataaattt tattcatcaa
     9961 cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa aattgaatat acaaaaaaaa
    10021 gacaacgtct tgcatattaa gtagttcagt tttggagtac aaaaagtgaa aatgtaaacc
    10081 taaatgagta tcaaattgca actgaatttg atactcgttt tttattgtta aatcaacgtt
    10141 tgttgttaat ttacaccaca aaatgtaagt taaaatgtat ataaaaatgc gaacgtgaaa
    10201 taattattgt aggaatgcaa attggtgtgt taaaatggtt acaatttact aagcaaaaag
    10261 gggtgcattt tcctatgcaa tttcttgagc caaaaaataa gaacgctaag tctgtggatt
    10321 gggaaatatc agagcaagta agggtgatag ttaagcaata cgctgaatat gcagagcgta
    10381 ctgaaagcga agcagtggat gaattcttat tgaacatttt agacgataag aagtttattg
    10441 aatggattgc caacaagcga agtaataagc ggattgttga aaaaatgggt atcaaagata
    10501 gagtgggttg atagacaatg accaagctta aaagattagc cacgaaggaa gatgaaattg
    10561 tagatgttaa aattccgatt tcaaacgaag aattaaaaga tagggctaaa caatatgatt
    10621 tattaactcc aaaacgattt gcaacaaggt ataacaaaat gttgttcttg ccaacgtctt
    10681 ttaaatggaa cggttctgaa tatcccattc aatataatta ctgcatcaat ccgttttgtt
    10741 gtaattttgg gaaagaacag cacaaattca aagatgtaaa agggaaacca agtcgatata
    10801 aaatgactgg tagttccaaa gataaaggtc ataaaggtat gtactgcaat gacaatccta
    10861 ttggcagagg tgtatcgcaa aactgtacag taaccccttt atctaattgg tctgttgttg
    10921 aagaaattaa acgccttata gaaatcaata gcatccaaga tgttgagcca gattatcaat
    10981 ttcataaaga aggctgctcg gaagaagaat caaccccatt caatgagccg aagcaatttt
    11041 acaaaagggg gaaaagtagg ggcaaatctc aacgctatca atgtaaggcg tgtaagaagt
    11101 tcactaatgt tctaccaaaa agagaagaaa ccactactta tcaccaacaa aagaacacca
    11161 ttcttccaat gttcgctaag atggttgttg gaagggtttc agtcagtcgt acttgtgata
    11221 ttttaggaat tggagttgga acctactatc ataagctgga gtggctgtat aggcgttgtt
    11281 tggagttttt agaacgatat gagacacaac cccttcaaac aaagaaattt aacgagatgt
    11341 ggcttaatac ggacaagatg cactactacc taaataacgt ccgtaagaaa ggacaaggtt
    11401 caaagaaata tacggggttt gaagacctta atatgcaaac gtatatagtt gtatctgctg
    11461 aggtcttatc aagatatgtg ttccgttctg atgttgccta tgattggaat atttcaatgg
    11521 atgaattaaa tgaagataca agaaaattca aagaagacca tctcaatact tttagtcgta
    11581 aaaatgatag gttagattgg tcttattatc cacaagaacc atccgctaac gatagcgaaa
    11641 ataggaatgc ttatcttcac gaattaggga aaattactaa taggagtaga tttgtcgatg
    11701 gtttaaatgt tgatgctcct tatactacaa ctgctcacta ttggttaatt aaacaaatgg
    11761 ttaatgccga cgaatggcga atgattagtg atgatgactt ctctattaga aacgcctttt
    11821 atagagtatt cacgaaagaa ttaaggttga gcgatgcaca tcattttatt tgccaagtta
    11881 acaagacgaa aagcagaaaa caatgcttaa aagaattcgg acaagcaaaa gcagaactgc
    11941 tcgattgggg agatatcaga ggattcaaaa caaagtttct acggactttg gcatctcatt
    12001 atctaactga attactaaca agtcatcaat tccacgaaga ggctattagt aaagatggag
    12061 aaagatatcg caaatatgcc gataacccta tcaaacatcc attagccact aaggataaag
    12121 gattttattc agttgattgt agaacggatt tatctgctct ggaaccaaac gaaatcgcaa
    12181 agatgctatt aaatgttaat gaccattcga ccaatagctt tattcaacag ataagacgtt
    12241 atatatcatc tcttgaaaga ccattaacaa cagctcgtgg tgataagaag agctatattt
    12301 acgctaactt caatccgaag tatgctcaat ttgcgattac aattctaaga acttactaca
    12361 acttttgtcg cccttttaaa tcagcggata aaaaggtttt gacacctgct caaagattag
    12421 ggattacaga taaacaattt gattggaaag atataattta tttcaaatga caaaaagagc
    12481 ttggagtaat cccaagctct tgtgctttat ctttactgtt cttcttcaat taacgcaccc
    12541 gttaactcaa taagtttttt ctttcatata catgaaaata gtagttatac ggatttttat
    12601 cattctttat ccctttttgg gcaaatacct cattccattc ctcataattc acttctggaa
    12661 aaaaagtatc tccttcgaat tcatgatgta tttttgtgat gtacattttc tcaacataag
    12721 ggaaaaacaa attataaatc tgttctcctc cgaaaataaa aatttcttct tcgtttttac
    12781 ataactcaaa aacatcttct attgaatgaa caatttcaca accattaaag gtaaacccct
    12841 tatctctcgt cagaataata tttcttctgt caggtaaggc tcttccgatt gattcaaggt
    12901 tcttcctacc taatattatc ggatgtccct ttgtagtatt tttaacatat tcccagtcct
    12961 tgggaatcct ccaaggaatg tcattctctt tgccaatcac tctattctta tccatcgcag
    13021 caatcaaaga aactttcatt tataacacag cctttttaac taatttacca tctaatttct
    13081 tatttctatc aaaaaacctt ttctcattat cctgcccgtt agttgaataa gcaattt
//
LOCUS       DARBER010000070        12935 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARBER010000070 DARBER010000000
VERSION     DARBER010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.071"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            414..947
                     /locus_tag="SCK06_001816"
     CDS             414..947
                     /locus_tag="SCK06_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG1151813.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="SCK06_001817"
     CDS             complement(1437..1961)
                     /locus_tag="SCK06_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151814.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="SCK06_001818"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="SCK06_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG1151815.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="SCK06_001819"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="SCK06_001819"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG1151816.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="SCK06_001820"
     CDS             complement(3683..4363)
                     /locus_tag="SCK06_001820"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1151817.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="SCK06_001821"
     CDS             4489..5358
                     /locus_tag="SCK06_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1151818.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="SCK06_001822"
     CDS             5384..5899
                     /locus_tag="SCK06_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151819.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6539)
                     /locus_tag="SCK06_001823"
     CDS             complement(5946..6539)
                     /locus_tag="SCK06_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151820.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="SCK06_001824"
     CDS             complement(6564..6743)
                     /locus_tag="SCK06_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151821.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="SCK06_001825"
     CDS             complement(6820..7329)
                     /locus_tag="SCK06_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151822.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="SCK06_001826"
     CDS             complement(7425..7805)
                     /locus_tag="SCK06_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1151823.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /locus_tag="SCK06_001827"
     CDS             8135..8884
                     /locus_tag="SCK06_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG1151824.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9068..10345)
                     /locus_tag="SCK06_001828"
     CDS             complement(9068..10345)
                     /locus_tag="SCK06_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151825.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="SCK06_001829"
     CDS             complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="SCK06_001829"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG1151826.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11267..12211)
                     /locus_tag="SCK06_001830"
     CDS             complement(11267..12211)
                     /locus_tag="SCK06_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG1151827.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12213..12899)
                     /locus_tag="SCK06_001831"
     CDS             complement(12213..12899)
                     /locus_tag="SCK06_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG1151828.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3728 a         2707 c         2295 g         4205 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     9121 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     9181 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     9241 gttgccattt ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     9301 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     9361 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     9421 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     9481 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     9541 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     9601 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     9661 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     9721 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     9781 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     9841 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     9901 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     9961 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
    10021 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
    10081 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
    10141 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
    10201 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
    10261 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
    10321 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
    10381 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
    10441 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
    10501 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
    10561 gtgagaatgg cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
    10621 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
    10681 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
    10741 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
    10801 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
    10861 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
    10921 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
    10981 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
    11041 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
    11101 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
    11161 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
    11221 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
    11281 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    11341 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    11401 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    11461 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    11521 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    11581 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    11641 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    11701 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    11761 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    11821 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    11881 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    11941 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    12001 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    12061 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    12121 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    12181 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    12241 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    12301 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    12361 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    12421 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    12481 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    12541 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    12601 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    12661 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    12721 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    12781 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    12841 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    12901 tattttcctc cataaaatga caatattttt ttaat
//
LOCUS       DARBER010000071        12886 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARBER010000071 DARBER010000000
VERSION     DARBER010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.072"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(113..757)
                     /locus_tag="SCK06_001832"
     CDS             complement(113..757)
                     /locus_tag="SCK06_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1151829.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(851..985)
                     /locus_tag="SCK06_001833"
     CDS             complement(851..985)
                     /locus_tag="SCK06_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG1151830.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(987..3119)
                     /gene="feoB"
                     /locus_tag="SCK06_001834"
     CDS             complement(987..3119)
                     /gene="feoB"
                     /locus_tag="SCK06_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG1151831.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(3116..3589)
                     /locus_tag="SCK06_001835"
     CDS             complement(3116..3589)
                     /locus_tag="SCK06_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG1151832.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            3990..4214
                     /gene="nrdH"
                     /locus_tag="SCK06_001836"
     CDS             3990..4214
                     /gene="nrdH"
                     /locus_tag="SCK06_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG1151833.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            4373..6532
                     /gene="nrdE"
                     /locus_tag="SCK06_001837"
     CDS             4373..6532
                     /gene="nrdE"
                     /locus_tag="SCK06_001837"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG1151834.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            6582..7547
                     /gene="nrdF"
                     /locus_tag="SCK06_001838"
     CDS             6582..7547
                     /gene="nrdF"
                     /locus_tag="SCK06_001838"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG1151835.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(7636..8775)
                     /locus_tag="SCK06_001839"
     CDS             complement(7636..8775)
                     /locus_tag="SCK06_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1151836.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(9084..9797)
                     /locus_tag="SCK06_001840"
     CDS             complement(9084..9797)
                     /locus_tag="SCK06_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG1151837.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(10051..10752)
                     /gene="nagB"
                     /locus_tag="SCK06_001841"
     CDS             complement(10051..10752)
                     /gene="nagB"
                     /locus_tag="SCK06_001841"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG1151838.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            10986..12518
                     /locus_tag="SCK06_001842"
     CDS             10986..12518
                     /locus_tag="SCK06_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1151839.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(12709..>12886)
                     /locus_tag="SCK06_001843"
     CDS             complement(12709..>12886)
                     /locus_tag="SCK06_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1151840.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4126 a         2607 c         2272 g         3881 t
ORIGIN      
        1 tcactttgtc aaaaatgaaa gaaatttgct tgtccaaact gaaaaaaact tggctaaatc
       61 tttgtaaata aaaggtttag ccaagttaat atttttttaa tatttcacac aatcattctg
      121 caagctgatg ctccgggtaa aaaaagtttg tcatacgctg tagtgtatat tcatttccaa
      181 taaaatgaac gatgtcccct gccgtaaatt tagcataggg gccaggtgaa acaagcagtt
      241 cttcattatg agaaattgcg ataactgtgg cgccagtcgc ctgccagata ttcaactcgc
      301 taattgaacg gtccagatgc attgcttttt ctgataaaga taattcaaac ggaagtaaag
      361 gattcatctt atgtattcgc ttcgtttgat tgaccagctg atccaataat tgagaaaaat
      421 gattcagctc atcttgttgc ttctccacac ttgccatgat ttctgctttt gtatcttgga
      481 tggattgtac atcttgatat tgcaatagga aatcttttgc tttttccttc gaagcaacaa
      541 atgccccact tccatgccgt acttccatga ttccaagatc taccagcaca ttgatagcct
      601 tgcgggtagt ctcaggagaa gcattgtacg tactggcaag tgttgagcgg gcatggatct
      661 tctcacccac tttatatcgg ttttcaacga tacgttcggc taaatcaaca gcaatctgct
      721 ggtagcgcgg gcggacgatt tttttctttc cagacatttt ctttcctctt tcttttttta
      781 ctttccctca tcttactatg ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg
      841 caacgactga tcatcgcttg atctgttcct tttttactgg gcaagctgtt tgacagtcat
      901 cacaattctt accggatttt aaacgagtat agacaattgc gcctgcactt ccaaagatta
      961 acacacttaa taagaatgtc gccatcctat tcctctcctt ctaatgacaa tatagtataa
     1021 aactggttct tggattttgg ctgacggaac aaaacataaa cgagtcctat catcagtaaa
     1081 atagcaagat acgtccctaa cgctacctgt ccaccctcca ataagtgtcc caactgataa
     1141 atcacaaaac taactaaata agctaaaccg cattgatagc ctatggcgat ccaagtccat
     1201 ttcttggcat tcatttcccg atggatagcc ccaattgccg caaaacaagg cgcacaaagt
     1261 aaattgaagg ctaacaatga ataagctgct gcgggagtat atgtgtgttg tagcacttgc
     1321 cagatttctc tgccattttc ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca
     1381 ataatatttt ctttagcgac caatcctgta atcgctgcga ctgttccttg ccagtttccc
     1441 caaccaagcg gctgaaaaac aactgccagg attcgcccga aaaaggctaa gatactttgg
     1501 tcttcagaaa caggttgagc ataaaaatta taagaagagc tgaaccagat cacgatacta
     1561 gaaacaaaga taatcgttcc tgctttttta acaaaagact tgctgcggtc ataggtttgt
     1621 cttaggaccg ctgaccattg aggaaaatga tatgcgggca attccatgat aaaaggagct
     1681 gtatctccag caaacaaacg tgtttttttc aagctgattc cagagaaaat aattgcagtc
     1741 ataccaagaa aataggcaga aggagctacc caagattgat gaggaaaaaa ggcccctgct
     1801 actaaggcaa taatcggcaa ctttgcagaa caaggcatga aggttgtgac catgatcgtc
     1861 aaacgacgat cttgctcgtt ttcaatcgtg cgactggcca tgacgcctgg cactccacat
     1921 cctgtagcaa tcagcatagg aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt
     1981 cgatccatga caaaagcgat cctcgccata tatccgcagt cttccagaaa gctcaagcat
     2041 aaaaacaata taatcaattg cggtagaaat cctaacactg ctcccacgcc tgcgatgatg
     2101 ccgttcaaga tcagctcctg catccatccc gctacttgcc attcagctaa caatctgcca
     2161 acatgttctg gtaaccatgt accaaacaat gtgtcattga tccaatctgt ccccattgtt
     2221 cctactgttt gaatagccag ataatagatc agccacatca caaatgcgaa gatcggcaag
     2281 gctaaccaac gattcgttgc aatctgatcg attttgtcac tcatcgataa ttggaaagaa
     2341 tcatttgaca caacacagag tgtacatagt tgggtaataa attcataacg ttcattgacc
     2401 agaatcgtct cactatcgtc gtccaatagt ttctctgtta actggatcag ttgctcgatc
     2461 tctttttgct gagaaactgc tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa
     2521 tatttcattg cataccagcg tttcttgccg ctttgttctc ctagtacatc ttcaatttca
     2581 tgtaaagctg cttctaaacg tggatcatat gtacagggaa caggaataga aggccgcttt
     2641 tttgcagact cctgaatctt ttcgattagt ttttcgattc cttgcttttt caatacgctg
     2701 attggtaaaa ctggtacacc aagaccatag gaaagctttt ctagattgat tttttttcct
     2761 tcttttttga tgacatccat catattcaga ccaatgatca taggaatgtc taaatccaat
     2821 aactgcgtag ttaaataaag atttcgttct aaattggtag catcaataat gttgatgatc
     2881 agttcaggct gttcctgaac caaataatct cttgcaatca cttcttctgg tgtataagga
     2941 gacaaagaat agatgccggg taggtcctga atatgaatgt tcggattttt tttcaacgat
     3001 ccttcttttt tttctactgt tacgcccggc caattcccta catattgatt cgaaccagtc
     3061 aagagattga acaagctagt tttcccgctg tttggatttc cgactaacgc gatattcatt
     3121 ctttttcctc cctttgtatc aagattagtt cggcctctga ctttcgtaac gtcagttcat
     3181 accctcgcac tgtcagttcg attggatctc ccagaggggc tagttttcgg ataactattt
     3241 ttacatttcg cgtcaatccc atatccatta accgtcgctt cactgcgcct tgtccgtgga
     3301 ttgctacgac gatcccagtc tctcctgctt ttaattgatc aagagaagtc cagctttcag
     3361 aggaagcagt ctttttattg acaaagattt gacctagtat ttcccgactt actgccactc
     3421 gatgactttt taccagtaaa atggcattgt tgcctgataa attgacgacg acgattttac
     3481 ttccggcaac gatcccgata ttttgcaaat ggcgttttgt tttttcttct gtttcgattc
     3541 gttcgacttg ataaacgaaa ttctgctttg cctgcgctaa ggtctgcata ttgattcctc
     3601 tcttgtgaaa taaatcatgt attaaaatca ttcattatat gttctaattt tcttacgtgt
     3661 tacgcaaata aatgagaatc attctcacat agaatactct cggtaattga gaatgtcaat
     3721 tatacttgtg atattatgag cgaatcttta gtattgatag ataaaaatga gaaaaaaata
     3781 tttcttcata gttttccaca attttttctc tattaaattt gctattttcc cacacgatac
     3841 ttgttttcag ctcttttttc aaaaacacaa aacttagtat tccacagttt ccacaacaca
     3901 atatgtagtg tttgttgttt gactttttta ctaagagttt tatactatct attatagata
     3961 ataagaatgt gttagaggag gcactcacca tgaacgtaaa aatcttttct aaaaataatt
     4021 gtatgcaatg taagatggtc aaacggtttt taacagaaaa tcaaattgct tttgaagaag
     4081 tcaatatcga tgaacagcct gaagcagtag actggttgaa agcacaagga ttccaaagtg
     4141 ttcctgtcat tacttctgat gcagaaacag ttatcggatt ccgtcctgat cagttgcgta
     4201 aacttgccag ctaacatgac taaaagaatc aaaacgtgat acatggattt gaagggcttg
     4261 agacagcacc agtttttgtc gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc
     4321 aatgatttgc aatcaagcat catcaaatac gaaatgaagg ttgggcgtac gtatgagtct
     4381 taaaaattta caagatgtca gttatttcaa gctgaataat gaaatcaatc ggccagtcaa
     4441 tggtcaaatc cctttgaata aggataaaga agctttagtt gccttttttg aggaaaatgt
     4501 caaaccaaat acgatgactt ttccatctat tatggaaaaa atcaattact tgatcgatca
     4561 agaatatata gaagcagaat tcattcaatt gtattcacca tcatttattg aaaagctata
     4621 tcaatttttg gacgagcaac atttttcttt caaatctttt atggctgctt acaaatttta
     4681 ttctcaatat gcgttgaaaa ccaacgacgg ttccgcttat ctggaaactt atgaagatcg
     4741 agtagccttc aatgcgcttt actttgcgaa cggggatgaa gaattagcac ttgctcttgc
     4801 agacgaaatg atccatcaac gatatcagcc agctacacct tctttcttga atgctggacg
     4861 taaacgtcgg ggcgaattgg tgtcttgttt cttgactcaa gtcactgacg atatgaactc
     4921 tatcgggcgc tcaatcaact ctgctttgca attgtcccgt attggtggcg gcgtagggat
     4981 tactttatca aatcttcgtg aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag
     5041 tggtgtcgtt ccagtcatga aattattcga agatagtttc agctattcta atcaattagg
     5101 tcagcgtcaa ggagctggcg tggtttattt gaacgttttc catccagaca ttattgcttt
     5161 cctttcagca aaaaaagaaa atgcagatga aaagattcgc gtaaaaactc tttctcttgg
     5221 cgtgatcgta cctgataaat tttatgaatt gacacgtaaa aacgaagata tgtatttatt
     5281 tagtccatat agtgtcgaaa aagaatatgg tgtcccattc tcttatgtaa atattacagc
     5341 tgagtatgat catctagttg caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga
     5401 tcttgaaaat gaaatctcaa aattacaaca agaatctggc tatccttacg taatcaatat
     5461 tgatacagcg aaccgcgaga atccaatcga tggcaagatc atcatgagta atttatgttc
     5521 agagatttta caagttcaaa cgccttctgt gattaacggc aaacaggaat atgaagtatt
     5581 aggaacagat atctcatgta acttaggctc gacaaatatc gtgaatttga tgcaaagccc
     5641 taactttggt caatctgtgc gtacgatgac tcgtgcattg acatttatta cagatgcatc
     5701 tgatatcgat gtcgtaccac cgatccaaaa cggaaataag ctgaatcata cgattggatt
     5761 aggtgccatg ggcttacaca cttatcttgc aaaagaacaa atggaatatg gctcagagga
     5821 ttcattagac tttacaaata tttacttcat gctgttgaat tattggacac ttgttgaaag
     5881 caacaatatc gctcgtgaaa gaaaacaagt tttccataat tttgaaaaat cagcttatgc
     5941 tgacggcact tatttcgaaa aatacgtaac tggagaattc cagccaaaat cagaaaaagt
     6001 caaagcattg tttgaaggca tttttatccc aactgctgaa gattggaatg ctttgaaaca
     6061 agcagtaatg aaagatggac tgtatcatca aaatcgcttg gcagtagcac caaatggctc
     6121 aatttcatat atcaacgata cgagtgcaag tattcatcct atcactcgca tgatcgaaga
     6181 acgccaagag aagaaaatcg gtaaaattta ttacccagcg ccttatctat ccaatgagac
     6241 gattccgtat tacacttctg cttatgatat ggacatgcgt aaagtaatcg atgtttatgc
     6301 aacggcacaa caacacgtcg atcaaggcat gagtttgact ttattcatgc gttctgaaat
     6361 cccagaaggc atttatgaat ggaagacaac aacgaagcaa acgacacgtg atttgaatat
     6421 tttaagacat tacgctttcc acaaaggaat caaatccatt tattacgtac gtacctttac
     6481 tgatgatgca gaagaaatcg gaagcaatca atgtgaaagc tgcgtgatct aatatcatag
     6541 atagattcgc ttattcatat tttaattaaa ggagcattct catgtcagaa acatattatg
     6601 aagcaataaa ctggaatgaa attgaagata tcattgataa atccacttgg gagaagctga
     6661 cagagcaatt ttggctagat acacgtatcc cactttcaaa tgacctagat gattggcgta
     6721 cattgtctca attggaaaaa gatacagtag gacatgtatt tggcggtttg acactgctag
     6781 atactgtcca atctgaaagt ggcatggatc agttgcgtaa agatgtccgc actccccatg
     6841 aagaagccgt tttgaacaat attcaattca tggaatccgt tcatgccaaa agttattctt
     6901 ctatttttag tacactgaat acgaaaaaag aaatcgaaga aatttttgac tggacaaata
     6961 cgaatcctta tctgcaaaaa aaagctgagc gtatcaatga aatctacaaa cacggtacac
     7021 cgctagaaaa gaaaattgcc agcgtctttt tagaaacatt cttgttctat tcaggttttt
     7081 atactcctct ttattattta ggaaacaaca aattggctaa cgtagcagag atcatcaaac
     7141 taatcatccg agatgaatct gtacatggaa cctacatcgg ttacaaattc caattaggat
     7201 tcaacgagtt acctgaagca gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt
     7261 atgagcttta cgaaaacgaa gaacgttata cagaagaatt atacgatcca atcggctgga
     7321 cagaagaagt caagacattc ctacgctata atgccaacaa agcattgatg aacctaggga
     7381 tggatccact ctttccagat actgccaatg atgtcaaccc aatcgtaatg aacgggattt
     7441 caacaggcac aagcaaccat gacttcttct cacaagtcgg aaacggctat ttgttaggtc
     7501 atgtagaagc gatgaaagac gacgattatt tgattggatt ggaataagga tttattttaa
     7561 ttcaaaaatt tttcgaaaaa taaaaagagt ttagcgtaca gatattttaa atgtacactg
     7621 aactcttttt tatgcttatt cacttgtttc ttccagcact tttggactgt ctaacttttc
     7681 cgaaacatgt tccattcttc tcaataaata gattttccgg atatttaata ttatcgtctt
     7741 aatcactgca tagacaggta tgcaaatcag catgcctaat attcctgcga cacttcctgc
     7801 tccaatcaat accaagatga tcgtcaaagg atggatattc attgatttcc caaataacaa
     7861 cggcttgatc aaatttccat ctaattgttg gatcactaaa atcgacaacg ccatatacaa
     7921 agcttgaaat ggagagataa acaaaccgac aatgattgct ggtaccgctc caataaaagg
     7981 gccgacatac gggataatat tcgtgattcc gcaaatgacc gcaagcaaca agctataagg
     8041 ctgatcgaat attctcattg ccaaaaagct cattacacca ataatcaaag catctaagat
     8101 cgtactgctg atataggcag ataatgtttc gtttaattct tttaccgttt ggcgtaactc
     8161 tttacgaatc gctttcggaa agaagtgaga caacgcatca ataaatttgt gcccatcctt
     8221 gaacataaat agcaaaataa atggaacggt aaataacaaa ataaaaaatt gcatcataaa
     8281 tgaaatgatt tttgagacac ttgcagataa actgactaat atgatattga acaaattact
     8341 aagagataaa tttgctgatg ctaattgttc ttccaaattg aaatttttaa actcttcccg
     8401 ctgcgccaat tcatttaacc atttgcttgt ttcatctgca tacattggca agctctgggt
     8461 caattgaatt gtctgttcca ttaactgagg cactacattc atgacagtta aaacaactaa
     8521 aatgattacg accaaaaacg tcaataaaaa accgaacact ctcgggatcc gatgcttttc
     8581 gagaaaaaca acgattggat cgaacatata atagataaaa ccagcaacaa gcaatggtaa
     8641 aaaaattgcc gaaagcattt tcaatattgg actaaaaata ttcggcattt gtaaaataaa
     8701 gtacacgccg atgatcgtga gtactagctc cactgtccag aaaaataatt ttgattgttt
     8761 aaaacgtgat tgcatacatc tcctccaagg tcctttgata cttctattct atcatttaac
     8821 aaagagttac gcgctgagat tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt
     8881 taatgagtag acgagtactt aaacaaaaac acggtttgga gtagtgacaa aaattatact
     8941 gcagtccctt tttccaaata aacggtgttc aaaagcttgc atcttcgtaa taagcccagc
     9001 caaacgaaaa atataaggaa ctatttttat ttggctgggc ttattacttg atgtaggaca
     9061 tctattttac aacctcagat catttattgc gcaatactga ctaaatgcaa ctttgtgata
     9121 tttttgtgta cggacaactc ctcgatcaca tcggcataag tgagcccttt tggaagatca
     9181 atcgtataga tatttttgta cactcggtag tcatcaacga attttacgtc aaaattcaca
     9241 tcttcgatta cgatattttt ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt
     9301 ctatggataa attggatttc taacttttta gtcgtaggta catggatcac gcgtttcact
     9361 actgtgagtg caatagcaat cccgataaaa ctgatgatcg caatggaata gtaacccatg
     9421 ccgatggcga tccctaatcc agcaattgcc cataaagaag cggcagtcgt aagtcccgtc
     9481 accgattgtt tcgtgacgat gatcgttccc gcgcctaaga aaccgacacc actcaccact
     9541 tgagcaatca gccttgcttc atctgaacga atcgttccag caagctgtgg atactctaaa
     9601 gcatctctta atgcaccact agcgatctca acttggatca atgcaatgat acatgctccc
     9661 atacaaacta aaatatgcgt acgcatacct gctggacgat ttttatattg tcgttcaaaa
     9721 ccgattgctc cgccaaatag catagcgatc cctaaacgta aaataatctc tggaattgtc
     9781 aataccatat cattcatcta aacaccctcc agtccaatat atcatgtctg aaaggagcag
     9841 acaaaaaaag tgcaattttt acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat
     9901 agtccttttt atgaataaac ggtgttcaat cagctgaccc tcggtattac tcaacaaacg
     9961 gcaaaatatg gggagctatt tttcctttgt cttctctaat actcaggtca aaacgctgat
    10021 ttcacaacct ctttagccta ctattgaaaa ttacaactta cttgctgctt cttcatcgat
    10081 gatcacaaca acattgtcgt gattttgcaa tacacttgct ggcaaatgat tcgttacagg
    10141 tccttcaatc atccctttga ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat
    10201 ttctttactt ttcataattg aaccgattcc cattgaaaca gcttttgttg gtacatcttc
    10261 tgctttatcg aagaatcgtt tgtttgcatt gattgttgat tctgttaagt caactactga
    10321 agtcgttccg tcaaatgaag caccaggttc attgaaaccg atatgtccat tttgcccgat
    10381 tcctaagatt tggatatcga ttggatgtgc atcgatgatt tgatcatact ttttgcatgc
    10441 tgcttccaga tcttctgctt ttccatctgg tacaaacgtt tctttgaatg gttttttatc
    10501 aaataattgg acatccatga aatgacggta gctttgttca tctgaaccgc ctagtccaac
    10561 gtactcatct aagtttacag aagtcatttc actgaaatcc aaatcgcttg aagtcatttc
    10621 tttatataaa gtaactggtg tgcttcctgt tgccaatcct aatacttttg cacctttgtt
    10681 cataccttct ttgatgattt caaatgcttt ttttccgcct tcttcagcgt tttttacacg
    10741 aatgatttcc atagtgattg ctcctttata ttttggtata gcccaattat agcattcttt
    10801 ttttcttttt acaataatta gtatagacca aattgcattt ttttcatttt cttgacaata
    10861 aataggaatc cgtgttaggt ttcataagat ggttcaatca aacaatccat gttgcatgaa
    10921 aaccaagcaa gtgattcact gtggacgctt tatgtctgca gtttttttat gaaaaaggag
    10981 ttattatgag tatactgaca ttaaacaaca ttacccagca atttggcgac aaaatcctct
    11041 atgaaggtgt cgatctgcaa ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg
    11101 gagccggtaa aagtacattg attaaaatca tcactggtga gatcttacca gatgacggac
    11161 agatccattg gcaaaaaaat attcacaaag gttacctcga tcaatatgtg gaagtcgatg
    11221 aaacattaac aatcgaagaa tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag
    11281 aaaaaattgc ttttctttac caaacatata gcgagacctt cgatgattcc ttgctggaaa
    11341 aagctgggaa actccaaacg gaattagacc aaggagtgtt ttatcaaatc gacaccttag
    11401 ttgctgagat gagcagcggg ttggggatcg atgtcttagg tttagattca ctgttgaaaa
    11461 atctaagtgg tggacaacgt tcaaaggtga ttttagcaaa actactttta gaaaacccag
    11521 atgttctgat cttagatgaa ccaaccaatc acttagatga tcagcatatc caatggctga
    11581 cgcagtttct tcaggatttt gacggtgcgt atatcgtgat ttcccatgac caagaatttc
    11641 ttgatcagat caccacccat atcgcagaca tcgagtttgg aaagattact aagtatactg
    11701 gccatctaaa acaagcattg aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat
    11761 atcatgctca acaaaaacaa atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg
    11821 gttcccgatc aacgatggca aaaagccgac aaaaacaatt agataaaatc gaacggctga
    11881 cgcctccagc ttcagcaagt aaagctgtct ttgattttcc attctcgcct attgtcacga
    11941 cattagcagt ggaaacaact gatttagtca tcggatatga tcgaccgcta cttgcaccca
    12001 tcaatttgac catccgcttt ggtgaaaata ttgccattcg aggcttcaac gggattggaa
    12061 aatctacttt attaaaaaca ctgatcggtg agattgaaaa aataagtggc gatttccact
    12121 tcccagataa cacaaaaatc aattactttt cacaagatct gtactgggaa aatccattag
    12181 aaacaccact acaatattta agcagtcaat ttccaaaagc gactatcaaa gaattacgtc
    12241 gccaactagc taaagcaggg ttagttaatc aattagcaag tgaacctctc actacattaa
    12301 gtggtgggga acaaactaag gtgaaacttg cacagttgac aatgaaccaa ggtaatttac
    12361 tgattctcga tgaaccgacg aaccatatcg atcaagaaac gaaagaaagc ttacaagacg
    12421 ggatacagaa atttccagga acagtaatca tcgtctctca tgaacaagaa ttttatcaag
    12481 atcttgtgca acgagtcatt gagattgaac caaaataaac gtttgattga atagttgaaa
    12541 aaaaggagct gtgacaaaag ttttgtcaca actccttctt ctgaataaat ggtggccaat
    12601 tagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg
    12661 ctattcttat tacttgatgc agaacagctt ttcacaacct cttttcgtct attttttatt
    12721 ttcgagattt ctctttcctt taaaaacaac tgtttgaaag aagaacgcac atccttgaaa
    12781 aacaagcccg ctaaccgtta cacctaacca gccatactgc tgccacattg tcgtccctag
    12841 caatgaacca gccgcacctc caataaagta cataaacata aataca
//
LOCUS       DARBER010000072        12823 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DARBER010000072 DARBER010000000
VERSION     DARBER010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.073"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            43..3570
                     /locus_tag="SCK06_001844"
     CDS             43..3570
                     /locus_tag="SCK06_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG1151841.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3567..7289
                     /gene="addA"
                     /locus_tag="SCK06_001845"
     CDS             3567..7289
                     /gene="addA"
                     /locus_tag="SCK06_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG1151842.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7358..7699
                     /locus_tag="SCK06_001846"
     CDS             7358..7699
                     /locus_tag="SCK06_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1151843.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8008..9051
                     /gene="pheS"
                     /locus_tag="SCK06_001847"
     CDS             8008..9051
                     /gene="pheS"
                     /locus_tag="SCK06_001847"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG1151844.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9056..11476
                     /locus_tag="SCK06_001848"
     CDS             9056..11476
                     /locus_tag="SCK06_001848"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG1151845.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11646..12656)
                     /gene="trpS"
                     /locus_tag="SCK06_001849"
     CDS             complement(11646..12656)
                     /gene="trpS"
                     /locus_tag="SCK06_001849"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG1151846.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4347 a         2236 c         2726 g         3514 t
ORIGIN      
        1 ataataaagg agcaccgtat catgtgttga aggaggaaaa gcatgtcttt acaatttatt
       61 cttggaaacg ggaaaaaaga tcaccgtcaa gtcttgatcg acgaagcatc cgcttggctc
      121 gaccaggatg caagtaacca agtatttttc cttgttccca actacagcaa attcgaacag
      181 gaacaagaaa ttttagcgga gatgcgaaga agaagcggaa aaaaaacttt cagcacaaca
      241 aacgttcaag tatttagttt ttatcgtctg gcttggtatt tgctgcaaca gacaactctt
      301 cttacaggaa atgaattatc agaatcagga tctgcaatga tcttgcgaca gattttagaa
      361 aaaaatgagg aagaattaac aatctttcgc ggagaaatca ataaaagagg gtttattcgt
      421 caattacaag aactctacag tgaattgcgt acaggaaata ttcaaccaga agacctgatg
      481 cttttatttg gtgattcacc taatgcagaa gaccagcaac ttaaaatgaa agacttgaag
      541 ctgatttttt cagcttatga cagcgaattg actcaacgtg cactaacaaa tgaagatgca
      601 ctatctatac tagcaaatta tatgcagaca caagatttca acaacgtcaa atttattgtc
      661 agtggttttt ctcgaatcaa tggtcaagag tatcaattac tgcaggcaat gatggaaaag
      721 gggcaattgg ttgtcgattt attgctggat cgtccttata cagcggatct tccagaagta
      781 cttacgctct ttcatgagac tggaaagctt tatttccgtc tttttcaaag tgcaagagaa
      841 cggaaagtcc ctatattagt ggaccgattt gcaccagagc agactcgtcc gacagagctt
      901 caactgctgc aaacaatgtg ggaagagaca tctagccaaa agaaagcggc agtacacaaa
      961 atagggggac aagagcatct ccatttgtgg gaagctgaaa atccaactga agaagtccga
     1021 caattagctg ttgaaatcag acgactagtc agtgaagaaa actaccgtta tcgtgatatc
     1081 caagtgctga caaaaaatat gtctttatat ggaaatatcc ttcggccgat ctttcaagaa
     1141 atggcaattc ctttttatat cgatgaagaa caaatgatgg aaaatcatcc gctgatcgaa
     1201 tggatcaaca gtctgttcgc tcttgaccgc tattcttatc ggctaagtga tattatgcgt
     1261 tttttcaaaa ctgaattatt tctgtcgacg gaagcagaaa ccgaacttga tgaatggaaa
     1321 caagagcgag atgaattcag gaaaaaactg gacatcactg aaaatgtagc acttgcttac
     1381 aattatcaag gaacttattg gactcgtgaa aaagactggc agtttgtggc gtatgatttt
     1441 gaagcagaac agattgaaga cgtacagtat ttggaagaac agtcaaatac gattagacga
     1501 ttatttcaga aagctgtacc agtatttttg aaaaaaataa agaaaagcaa aacaggacaa
     1561 gaggccgcaa atgtttttta tcaatttttg atccaaagtg gcgtacagca acaattattg
     1621 ttttggcgta atcaggaagt agacagagga aacttagaag cggcaagaaa ccatgagcag
     1681 acatggggag cgctgattga tctgttggat gaatatgtag taatttatgg tgaatcggct
     1741 tttgactggt cactgttcca agaaatcatt acaagtggtc tagaaaatct aagttatggt
     1801 aaaatcccta ccgcaatcga ccaagtgcga atcaatcgtt tggaattagt tcgaccaaat
     1861 caagctgccg tcacttttgc attagggttg aatgatcaag tgtttccaga ccgaaaagag
     1921 acaaaaggat tgttgtccag tgaagaacga gaagtattaa atcaggcctt gccagatgga
     1981 cagtttttat tcgacccatc aaaggaaagt atctcttttg agcctttcca agcctatctt
     2041 gtttttcttt ccggaacaga tcgattatat cttagttatg cacaaagcta tgacacagat
     2101 ggttcgctga agatgtctcc ttatttgcgt agaatcacag aatatttatc tgttcccgtc
     2161 gagagaaaag agcatttgac gatcgaaagc gaccctaatt gttatgtagg gacttacaga
     2221 agcggaatca atacagtcaa taggatttat cgattagctt tagatgaaaa aaaacctgta
     2281 ccaaagcact ggcaaaagct caaagaatta gtactgaatt cgtcatggcg aaattttgct
     2341 ttacagattt ttgaaagtca ggaacattta aatgttcctg tttcgatgcc agaaaaaatg
     2401 gcggaagagc tttatggaaa agagatttat acttcaattt cacgtatgga gaatttttat
     2461 aattgtgaat acaaatactt catgcaattt ggtttgaaat tgaaagaacg aactgtttat
     2521 ggtctaactc cagcagcaac tggtgatttt tatcatgaat cattggatcg tttcttcaag
     2581 ctactgtttt caaaccaatt atccttagtg aatatgtccg atgcgcagcg tagagacttt
     2641 actgaaaaag tgctgcagga agtattcgga gaacttcggt ttgagatatt ggatagttct
     2701 gcaagaatga actatatccg ctaccagtta ggacagacga ttcaaaaagt gacatgggct
     2761 ttgcaaaaac agagcaaaaa tacaggcatg catcctcttc agacagagat tctatttggt
     2821 cagattgctg gagcaaaagg tatcacaggt ctggaattac cgttgagtaa tggtggaaaa
     2881 ctccatgttc gagggaaaat cgatcgaatc gatgtagctt ctgaacaaga agatacttgg
     2941 ctaagcgtcg ttgattacaa atctagcgga cgttcattcg atgtcacaga agcttattat
     3001 ggcatggcta tgcaattatt gacgtatctg gacgtagcat tgatggatgc ggtacgtttg
     3061 actggaaagg cggctgttaa accagcagga tcatattatt tacatgttca taatcctgta
     3121 ttgacggaga cagatgatgt agagaagaat acattgaaaa aatttagtta tgatggcgtt
     3181 tttgtcgatg atcccgctct tttagaagta ctagatcatt cactggaagc aaaagaacaa
     3241 tcactgatct atccagtgaa aaaaaatgcg aaagatgaat accaaaaagg tagtttcagt
     3301 aaagacaaat ttttcacgga agaagaactg caactattca tggcgcataa tcgggaaaat
     3361 atgcggttag ctggagatgc gattacttcc ggagaaatca aactaaatcc agcttataaa
     3421 gataaacagc ggattgcctg cgaattttgt ccattcagaa gcatctgtac gtttgatgtg
     3481 atgctgaaag aaaataatta ccaccgtctg gagaaaatag ataaggaaga agcactgcgt
     3541 agaatattga aaaggagtga agaagagtga atcaaattcc tttaaaaccg caaaatgaac
     3601 gattcacaga tgatcagtgg caagcgattt ttgatcaagg agataatcta ttagtctctg
     3661 cgtctgccgg atcaggtaaa actacagtgc ttgttcgaag agtgattgaa aaactaaaaa
     3721 tgggttttga tatcgatgaa ttattgattg tgacatttac tgaagctgcc gcaagagaaa
     3781 tgaaagaaag aattcaagag gcattacaag aatccgtcaa tagcgaaagt gaccctgtac
     3841 gtagacagca ttttactaag cagttagttt tattgcccac agcgaatatc tcaaccttac
     3901 atgctttttg cctgacagtt attcgacgtt attattactt aattgatatt gatccagtct
     3961 ttcgcatgtt aacagatgaa acagaaacga tcttgatgaa ggaggatgtg tgggacgagc
     4021 tgagagaagc actttatgca gaaaatgatg aacgattttt ccaattaaca atgaattttt
     4081 ctaatgatcg ttcagatgac ggcttgacga atctggtgtt ttctctttat gagtttgccc
     4141 gtgccaaccc tgacccccaa aaatggctag aacagttaag cgataattat cgtttgcctg
     4201 aggggctagc gaaatctcga ttatatcaaa aacaaatccg tcctttagtt ttggcagata
     4261 tataccaatg tgtacagtta tatgaacaga tgactcagtt agcacaaggt gaaggattag
     4321 aaaaaatgca tgagcaagta gctggagaac aacagcagat caagaatatc tatgaagcgt
     4381 tctcgcaaga tcggttagaa gaagcttatg ctggtttgga acaactaact ttttcaactt
     4441 ttaagagcag tcggaaagca gaattgaaag aaatttccaa tgaagtcaag ggcatgaggg
     4501 ataaagcaaa aaaactgatt cagcagataa gtaaaagtta ttttcctgtt tccccaagcc
     4561 aaatggaaga attaacggac aaagcactac cgttagtcga agaaatgaca aaagtcacgc
     4621 aaagcttcat ggacggtttc agtatgcgta aacgggaaaa aggtgtgctg gatttcaatg
     4681 atctggaaca tttagcgttg cagattctca cagaaaaaac gaaagatgta tggctgccaa
     4741 gtgaagcttc aaaacattat cgaaaaaaat tcaaagaagt aatggtagat gaatatcaag
     4801 acgtgaacca attacaagaa gccattttat actggctaag agaacccgat gatacaaaag
     4861 gaaatatgtt catggttggg gatgtcaaac aatccatcta ttctttccgt ttagcagatc
     4921 ctagtttatt tatcgggaaa tacgagaatt tctcaaagaa agaaggcgga cgtcgaatcg
     4981 ttttggctga gaacttccgc tctagaaaag aagtcctttc ttttacgaat cttatttttg
     5041 aacaactaat ggatccagca gtgggtcaaa tcaattatga cgaagcagcc aaactgatcc
     5101 aaggattttc agactttcca gagaatgaac agtttgagcc ggaaatcatg atctatgaaa
     5161 aagaacagga agaatcagag attgagattc cgacggatga tatactggaa gacaaaacag
     5221 aaggcgaact ttttatgaca ggactaaaaa ttcgtcaatt aattgattcc tcctttatga
     5281 tttatgataa aaaatcaaaa aaaagtcgtc cgatagagta caaagacatt gttctcttga
     5341 cacctacaaa gaagaacaac ttaacgattt tagagatttt caaaacattg gatattccat
     5401 tagagatgaa tgatgctcaa aactattttc aagcgacaga aattcgcacg atgatctcgc
     5461 tgctacaatt aatcgataat ccctatcaag atattccgct tgcggctgta ttgcgctcac
     5521 cgatcgtggg tctgattgaa ccagaattag ccagcattcg attagctaac cgtgcacata
     5581 cttactatga tgctgtgctt gcttatcaag catcaaacga agatgaatta gcagctaagc
     5641 tggaacattt tggcaagcaa ctagagcatt ggcgagaact tgctcgccgt tcttcgatca
     5701 ctgacttgtt atgggatatc tattatgaga cgggatatct agagtatgtg gtcggtctgc
     5761 cagcaggggc acagagacaa gccaatcttt atgcattggt tgatcgtgcc aaagcttacg
     5821 agcaaagttc tttcagagga ttataccagt tcgtgcgttt cattgaaaaa atgcaagaaa
     5881 aagataaaga cttagcagaa ccagtcattt caatagaaga taatgctgtt cgcgttatga
     5941 cgatccatgc aagtaaaggc ttggaattcc cagttgtatt tttgttagat atgacaaagg
     6001 agttcaactt acaagacttg cgcaatcggt atgcttttga agaaaaactt ggagcaggaa
     6061 ttcgctatat ggatcctgaa acacgagtgt tatatgatac gttaccgttt caagccatca
     6121 aattggcaaa gcaaaacaaa ttgttgtcgg aagaaatgcg taaattgtat gtaggattga
     6181 ctcgggcaga acaaaagctt ttcattgtcg gttcctataa aaataaagag caaatgatcc
     6241 aaacgtggtc tgaagcagct gatcatgagg agttagtttt tgatcctgcg ctacgtttga
     6301 agggcagaag cagcttgatg aattggattg gttacggatt gatccgtcat cctgaaatgc
     6361 agaaatactt agaggaagaa atttcgactt ctcttcttca acattcaaat gcacagtttt
     6421 ctatttcatg gatgaatcag caatcgatca ttgaacaaag acagttgctt gctgaaaaag
     6481 aactggttaa cttagatcag cagatgaaag aagatgaaac tttattagca gattcgttac
     6541 aaaaacgtct ggcatatgaa tatccttatc aagcttcttc acaaaccaca agttatcaat
     6601 ctgtatctga aatcaaacga ttattcgaag atccagatga tacacaagaa tcacgtttga
     6661 ctctggaaag cagtcagaac aaagcagcat cccgtcaatt tcgttatacg caagaacaac
     6721 tagcagaacc aaaatttttg caaaaagatc ggcaagtgtc agcagctgct gttggaactg
     6781 ctacgcatgc tttgttacaa ttactgccat tagagatgcc aactacagag agtatccatc
     6841 aaaaattaca ggaattggta aggaaacggt tagtagacga aaaagtcgcg aaaaaagtag
     6901 atgtttcttc tattatatgg ttctttcaaa cagaacttgg tcaacagctg attgccaaca
     6961 aagaaaacgt aaaacgagaa caaccttttt ccatgctgct tcctgctgac gaagtatttc
     7021 aagactatcc aaatcaagaa gatgaactat taatccacgg aatcgtcgat ggatacctag
     7081 aagaaaaaga tcatctcaat atctatgatt ttaaaacaga ttttatttta catccggatg
     7141 atccagcaga aatagatgca atcgtccaaa aatatcaagg acagctaaga ctttaccaac
     7201 aagcaatgtc agaagctttg aataagccag tagaaaatgt tttcctaatt ttgcttcgag
     7261 tgaaacaaat tataaacatt aacaaataat tataattgag caaacttgac ttatgttttg
     7321 taaggattat gctatactct gggaagaggt gagtatgatg gagctaacac agacagaatt
     7381 cgttttatgc cctaaatttg aaaaaacatt ctctatttta ggaaaaaaat ggaacggatt
     7441 gattatcgat gtactactcg aaaacggtcc gcaacgcttt gttgagcttg cggcaaaaat
     7501 cccgatggtc agtgatcgag tattagtcga acgcttgaag gaattagaaa aagaaggaat
     7561 agtcactcga aactttgcct gtagcgaaaa gagccgttca gaatatctct tgactgaaaa
     7621 aggagcagac ttgcaaaaag cgatgcgtca aatacaagtt tggagcgaaa aatggatcac
     7681 agaggaagaa tgcggttgat tttgtaaaaa aaatcttgta aactatagtc catacgaata
     7741 agtaaagaac gatgacgaaa aagagtagtt tggctaaacc agttcaggga gatctgtcgc
     7801 agactgaaag cagatcctgt gtttgaaaaa tgaagttcac tttccgagtt cgtctgataa
     7861 aatcagaccg gcaattgtcg ttaacgaagt aagagtgcgg tgtaagtcat ttatccgcaa
     7921 attaggatgg taacgcgaag acctcgttcc ttaacaggaa tgaggtcttt ttgttttgta
     7981 tttggaattt aaaggaggaa caagcacatg tctctacaag cacaattaga gagcttgaaa
     8041 gaagcaacgc tcgtcaaaat cacagcggca caagatttaa atgaattaaa tcaaatacgt
     8101 gttgaaacgt taggaaaaaa aggaccgatc acagaagtat tacgtgggat gaaagatcta
     8161 agtccagaag agcgaccaaa agtcggaagt ttcgcaaatg agatccgaga tcttttgaca
     8221 gaagagatcg agcgaaaaaa acaagtatta gaagaagaag cgttaaatgc agctttggca
     8281 aaagaaacaa tagacgtgac actacctggt agaaaagtaa aacatggtac acgtcatatc
     8341 gtgacacaaa tcatggaaga aattgaagat atttttgtgg gtatgggcta tcagatcgta
     8401 gaaggatatg aagtagaatc tgatcattac aatttcgaac ggatgaatct gccaaaagat
     8461 catccagcaa gggacatgca agatactttc tatatttcag acgagatatt gattcggaca
     8521 catacttcac cagtccaagc acggacaatg gaaaaacatg atttctctaa gggtgctcta
     8581 cgaatgatct cgccagggaa agtcttccgc agagatacag atgatgcgac ccacagtcat
     8641 cagttccatc aaattgaagg gctagttgtt gataaaaaca tcacaatggg cgaccttaaa
     8701 ggaacattag aagtcgtaat gaaaaaaatg tttggggaag atcgtgaaat tcgtttgcgt
     8761 ccaagttatt tcccatttac ggaaccatca gtagaagtag atgtcagctg tttcaaatgt
     8821 ggtggtgccg gttgtaacgt atgtaaatac actggatgga tcgaaatttt aggagctggc
     8881 atggtacacc cgaatgtgtt gaagatgtca ggaatcgatc cagaagaata ttcaggcttt
     8941 gctttcggcc ttggaccaga ccgcgtagct atgttgcggt atggagtgaa tgatatccgt
     9001 aatttctatc aaaatgactt acgtttctta agtcagttca agggggagta agaaaatgtt
     9061 agtttcatat aaatggctca atcgttatgt agatttatcg aatgtaacac caaaagaatt
     9121 agcggataag atgtctgtga caggaatcga agtagaggga gtaacggttc cagaagaagg
     9181 cttgaaaaaa atcgttgtcg gtgaagtcaa ggaatgcgta ccacatccag attctgatca
     9241 cctttctatt tgtcaagtcg atattggcga agacgaactg tctcaaatcg tttgtggagc
     9301 gcctaatgtg aaggttggaa tcaaagtgat tgttgcactt cctaactcac gtatcgctgg
     9361 taatgtaaaa atcaaaaaag gaaaaatgcg tggccaagta tcaaatggca tgatttgctc
     9421 attacaagaa attggctatt cagatagtgt tataccgaaa gaatacgctg aaggtatcta
     9481 ttatatgcca caagaagctg taaatggcga tccagttttt ccttatttgg atatggatga
     9541 ttcgattatt gaattatcta tcacaccgaa tcgtgcggat gcattgtcaa tgcgtggcgt
     9601 agcttatgaa gttggggcaa tttatcgtca gactcctaca ttcgatgacg aaaagctggt
     9661 agaatcctca cgtcctgcta gcgataaaat tcaagtggaa gttgaagata aagaagcagt
     9721 tcctgcttat caaatcagaa tcattgaagg agtaagaatc cagccaagtc ctcaatggct
     9781 gcaaaatctt ttgatgaacg aaggcattcg tccaatcaac aatgtagtag atgtgacgaa
     9841 ctatattttg cttttattcg gacaaccact acatgcattt gactacgaca aattgaaatc
     9901 aaaagagatc tttgtccgcc gtgcaaaaga aaatgaaaca ttagtaacac tagatggaga
     9961 atctcgggaa ttgacaactg acaatctcgt cattactaac ggcaaagaac cagttgcttt
    10021 agctggtgtc atgggagggt tagactcaga gatcacgaat gaaacaacaa cagttgcact
    10081 agaatctgct ttatttgatc caatttcgat tcgtcgtaca gcaaaacaat ttaatttgcg
    10141 tagtgaatcc tctgcacgtt ttgaaaaagg aatcaaccat gcgactgttg gagaagccag
    10201 tgaggttgct gcagcaatga tcgtttctct tgcgggtggg gaagttctac aaggcgcggt
    10261 caaaggctca gagatcaaag cagaagacgt atcagtgaag atttcacttg aacgcatcaa
    10321 tcaatattta ggaacatctc ttgtcgttaa agaagtaaat gagatttttg aagcacttgg
    10381 atttagctat gaagaaaagg agtatgttta ttccgtagta gtaccaccaa gacgctggga
    10441 tatccaaatc gaagcagata ttatcgaaga agttgctcgt atttatggat acgaccagtt
    10501 gccatctaca cttccaagtg gagaaacggt tgctggaagc ttgtcaaaag aacaagaaac
    10561 tactcgtaaa attcgtacaa tctttgaagg aagcggattg agcgaagcaa tcagttatgc
    10621 attgacaaca gaagaaaaat caagacagtt tgttataaat gaaagtaata ttacacgttt
    10681 ggactggcca atgagcgaag aacgttctgt attacgaatg aatttgatca gcggactatt
    10741 agatgatatc agttataaca ctgcccgaaa aaatacagag attgctttat atgaaattgg
    10801 tcgggtgttc ttccaagaaa atgatccacg tacacatctg ccgcaagaag taaaacatgc
    10861 agcaatcgct atttctggta tttgggaaga aaaagattgg cagacgaaag ctcaaaatgt
    10921 agactttttc accatcaagg gtcttttaga aaacttattt gaacaactag gcatcactga
    10981 agatgtccat tatcaaacag tgacagaaat gaaggaaatg catcctggaa gaactgctgc
    11041 gatttacctt ggcgaaaaat ttattggttt tgtcggtcaa gtgcatccaa cgattacaaa
    11101 aagttatgat attccagaaa catatgttgc tgagtttgat ttatctgcta tagtggaagc
    11161 tgctcaaaaa gggatcgttt tcgaagcagt gacaaaattc ccagcagtca gccgggacat
    11221 cgctttgctt gtaaaagaaa ctgtgacaaa tcaggaatta acagaagtta tccgttctgc
    11281 tgccggaaga tttttaacag atatccaatt atttgatgtc tatcaaggag aaaacattga
    11341 aaaaggacat aaatccatgg cgtatagcct gacattcgtc aacccagaag caacattgac
    11401 agatgaagaa atcaataaag caatggaaaa agtcgaaaaa gcgttggtag aaaacctaga
    11461 ggcaagtatt cgctaagaag gaaaatatag taagtaacga tcaagtagct tttgaagacc
    11521 atacatttga aaaaaagatg tgacacaact tcgtcacatc tctttttttg attttaaaaa
    11581 aggaatagca cagagacgag ataaaaaaac aggcctttac agcctgcttt tttagagtaa
    11641 atgaattaat ggaagtaatt gattcccatt gctgatttta cttcagaaag tgtttgtgca
    11701 gctactttag cagcttcttc actaccttta cgtaagattt ccataacagc tgaaggatct
    11761 ttttccaatt cttctcggcg cgcacggatc ggtgcaaatt cagcttccaa tacatcaatg
    11821 aggtatcgtt tgatctttac atcaccaagt ccgccgcggc ggtaatgagc tttcatttct
    11881 tcaattgctt ctttatctgt tccgaataca tccaagtagg tgaagaccat attcccttct
    11941 acttgtccag gatcttgtac atggatgtgg tttggatcag tatacatact catgactttt
    12001 ttctgtaaga catcggcagg atcggagata taaataccgt ttcctaacga tttactcatt
    12061 ttgccatttc catcgattcc aggtaatcta cccattcctt ttggtgggaa tacgcctttt
    12121 ggttccacca agacttcacc atatgtatga ttgaagcttt gtacgatttc ctgtgtctgc
    12181 tctagcattg gtttttgatc ctcaccaaca ggtacaagat tcgctttgaa tgcagtgata
    12241 tctgctgctt gagaaacagg atagataaag aatcctgtag gcacgctttc accgaatttt
    12301 ttttgttcga tttctgtttt gacagtcgga ttgcgccgaa cgcgtcctac gctgactaag
    12361 ttcaaataat acatggttaa ttcagctaat tccggaattt gtgattggat aaataaagta
    12421 gattttgcag gatctaatcc cacggctaaa tagtctaaag caacttgcaa gacatttgat
    12481 gaaaccttct caggattttt agcattatct gtcagtgctt gcatgtcagc aatcatgaca
    12541 aacagttggt tgtcttcatc tgcctgcata gccactcggg tttttagaga accgacgtaa
    12601 tgccctaaat gcagcttccc agttggtcga tctcccgtta aaataatatt tttcaaaaaa
    12661 gacacctctt tacgatttgt aagtctgagc acttaatatc ttaccgtttt tcctgaaaag
    12721 aaacaacagt tcctaaggaa aaaacaagga aagaggataa aataactaat agattagaaa
    12781 tggaagaaat atttatggga gaataaacgt tctaagaaac ttt
//
LOCUS       DARBER010000073        12817 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DARBER010000073 DARBER010000000
VERSION     DARBER010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.074"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            81..2246
                     /gene="acm"
                     /locus_tag="SCK06_001850"
     CDS             81..2246
                     /gene="acm"
                     /locus_tag="SCK06_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG1151847.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2518..3003)
                     /locus_tag="SCK06_001851"
     CDS             complement(2518..3003)
                     /locus_tag="SCK06_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG1151848.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3093..3438
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3483..4124
                     /locus_tag="SCK06_001852"
     CDS             3483..4124
                     /locus_tag="SCK06_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1151849.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4143..4778
                     /locus_tag="SCK06_001853"
     CDS             4143..4778
                     /locus_tag="SCK06_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1151850.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4796..5611
                     /locus_tag="SCK06_001854"
     CDS             4796..5611
                     /locus_tag="SCK06_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1151851.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5930..7366
                     /locus_tag="SCK06_001855"
     CDS             5930..7366
                     /locus_tag="SCK06_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1151852.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7555..10002
                     /locus_tag="SCK06_001856"
     CDS             7555..10002
                     /locus_tag="SCK06_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1151853.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10232..11299
                     /gene="rlmN"
                     /locus_tag="SCK06_001857"
     CDS             10232..11299
                     /gene="rlmN"
                     /locus_tag="SCK06_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1151854.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11492..12595)
                     /locus_tag="SCK06_001858"
     CDS             complement(11492..12595)
                     /locus_tag="SCK06_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1151855.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4380 a         2151 c         2733 g         3553 t
ORIGIN      
        1 gggggaggaa ttgaagaaag gatttttgag agatgatata gtaggtttta tttttagtta
       61 aaatagggga gatagaaaaa atgaaaaagt gtagaaatat tattttatca atgttattta
      121 ttattactaa tataacatca ctgattcctg ttcatgtcta tgcggatgca ggcagagata
      181 tcagcagtaa tgtcacttcg ttgaccgtgg acccaacgaa tattacagat ggaggaaaca
      241 taaaagtaaa attctcgttt gacgaaaaaa aacaaaatat ccaaccaggt gattatcttt
      301 ggatcaattg gccaagtgaa ggaaatattc gcggtgaagg attccaaaaa gaaatcccat
      361 taatgattga aaataaaaat gttggaactt taacggtcag aaaagattct gcgcaggttg
      421 tgtttaatga aaatattaaa aacctagact cagtagaagg ttgggggcaa tttgagatac
      481 aggccagaaa cgtaaccgat accggtgaag aaaatacggg accattcacc gtgacgagcg
      541 gtgataaaac agctactgta aatgtgacaa agccagcttc tggttcttct tctagcgttt
      601 tttattataa aacaggagac atgctacctg aagatactaa gcacattcga tggtttttga
      661 atattaacaa taacggcact tatgttgaac aaccagttaa aatcagtgat gaaatccaaa
      721 gtggacaaag attagatcca agtacgtttg aaattaatca aatacactta ggtgaacaga
      781 aggtttatag aggagaagag ggaatccaac aatttttaca agatttcccc agcgctacat
      841 ttaatttttc agtaactgat aattacattg aaataactat accaaaaaac tttgtcaatc
      901 ttaggaaaat aatggtgagt tataagacga tcatagaaaa cccagaacaa ataaattttg
      961 agaatcattc ggaagcttgg tttaaagaat ttaataaacc tgctgttgat ggagaaagtt
     1021 tcaatcatac agttaaaaat atttcagctt ctggtggagt caacggtaca gttagaggcg
     1081 aattgaagat tttcaaatat atcaatgaca cagagattgg tatcccaaat gtgactttcg
     1141 aattaagacg agcagatgaa cagcctattc aaggacagtc tagtatcctg ttgacttcga
     1201 atgaacaggg agaagctagt atcaaaggcc tgcaagtagg agactatgtt gtaaaagaaa
     1261 aagaagcacc caattggatt gattttgatc cactaagttc aaatgaactc aaattctcca
     1321 taaacgaaaa tgatacagag ggagtcagtt tacctattta taacaagaaa aaggttacga
     1381 acatcacagc aactaaaatt tggaacggtg gaaccactcc aagaccgagt atttatttta
     1441 aattattcag agcaagtaca aacaattggg aaccagttcc tgatgcagaa acaaaacgat
     1501 tagacaatgg aataacttca gtgacttggg aagatatcca acagtatgat gatagtggaa
     1561 acgaatatac gtttaaagtg caagaagtag atcaaaacgg aaatgattat gtcccttcag
     1621 gttatcaaaa aattgaaaac gggctagtcg ttacaaatga gaataaagaa gtcatttcag
     1681 tctcaggaca aaaaacatgg gaagacaatg agaaccaaga cggcaaacga ccgacgacga
     1741 tcacagtaaa tctattagcc gacggtcaac cgatccaaca caaagaagta agtgaaaaag
     1801 atgattggcg ctatcgtttc acaaatctgc caaaatacag agacgggcaa gaaatcatct
     1861 atactgtaac ggaagacaac gtgcccgaat acagtacgac gattgacggg tataacatca
     1921 aaaacagtta tacaccggaa aaaacaaata tatctatacc aggtagccat ataacaccgt
     1981 ggaaaaattt tccagagaag acagagagag agaaaattag aaatctattc tctcaaaaat
     2041 tgcaaatatt tgctcataca ggagaatcca gcgaacaaag aagcaattat attagtaaga
     2101 gagctgtacc taaacagata acaaataaat caaaaagcat tcttcctaaa acaagtagta
     2161 aaaaatctag ttttgctgta atcattggtt tattgatagt aactatctgt gctgggatat
     2221 tcttacaaaa atataaaaaa gtttagttag aatataatat tttttgttaa atatgactga
     2281 aatcaaaaga ataaaattcc gatttataat cgaaaaaaga agcattttta tatatgcttc
     2341 ttttttcgta gaatcaataa atatggattt ggctaactat ggaagaatac tcgtattagt
     2401 tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt ctgatatatt
     2461 ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac tctatcatta
     2521 caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa
     2581 atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat cttgacgatc
     2641 aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca atgctaatgg
     2701 agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga
     2761 taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct tttcatcagg
     2821 caagatgaca agatagattt gttttccttt tcttgatttc aataatagat tttttacttg
     2881 cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat gatcgacttt
     2941 ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg ttgcacgact
     3001 catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg acaaatgaaa
     3061 aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga agagtagccg
     3121 ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac cgtgaaacac
     3181 atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc gttacagctg
     3241 cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg taaaaaaagg
     3301 tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg ttttcatctg
     3361 aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag gtggaaccac
     3421 gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag gaggaaaaga
     3481 agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc acaacattga
     3541 aattattttt atttaccttg attggctcat taccattagg gattgtttta gcattaggat
     3601 tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg gttatgcggg
     3661 gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg attgggatcg
     3721 tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca gcttattttg
     3781 cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag
     3841 ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa gtgacaaaga
     3901 ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct tccttgatct
     3961 atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga
     4021 ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt attctaacaa
     4081 tcataatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata ggaggaagaa
     4141 atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata atcaaagatt
     4201 tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc ggtggcggta
     4261 aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga gaattttatt
     4321 tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg
     4381 tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt accttagcac
     4441 caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa gaattagcgg
     4501 cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct ggcggacaaa
     4561 aacagcgggt agcgattgcc agagcattgg ctatgaatcc acgggtattg gcttatgatg
     4621 aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg atcctatcat
     4681 tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt gcagaaaatg
     4741 tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga tagagatgaa
     4801 aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa
     4861 caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg
     4921 tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc tcacaggatt
     4981 tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag actttcagcc
     5041 cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt tgatatggaa
     5101 cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac cttatatgga
     5161 aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg gacaaatgaa
     5221 taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta cgaaacaacc
     5281 agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata gttttaatga
     5341 agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata aagtatacgc
     5401 agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa aaggttctta
     5461 tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag tgaaaaaaat
     5521 caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat ccgataaatg
     5581 gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca aaagacgtgt
     5641 cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg
     5701 aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt cggtattaag
     5761 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac tactcgaaac
     5821 taagcgactt tttcacaacc tcctttacat aattttattt tgttgtggaa ggggtttcgt
     5881 gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa tggaacacaa
     5941 agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag cgtatcaagt
     6001 agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat ttgtacggat
     6061 acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact atcatcgtta
     6121 taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc
     6181 ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc tcgcttttta
     6241 tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga ctttatatca
     6301 ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac gtcagatcat
     6361 tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga aagtccatta
     6421 ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt tagcagcaca
     6481 tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg ctaatttagc
     6541 aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa tcggaccaag
     6601 ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc tggctgctga
     6661 aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg gaaaatatcc
     6721 gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg aagctggcga
     6781 tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact atcaaacggc
     6841 gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata cgacaggaaa
     6901 aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg agaatccatt
     6961 tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga tcggactgcg
     7021 tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct taggagagta
     7081 tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt acttacaaag
     7141 ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag gctattgcac
     7201 ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat atggatttgt
     7261 atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga aagacagtta
     7321 ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca catacttaaa
     7381 acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt tagaaattaa
     7441 gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc agttctttca aacaaaactt
     7501 tagttccctt actttttttt gtataatgaa acttgatgaa atgagggata ataaatggca
     7561 caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag
     7621 cagcaggaac aaatgctgac gattggtgtt gggtttgcct tgattttatt tgctgtcttc
     7681 ggctttttaa aattaggttt tttaggaata ttgattgcaa acggctttcg gatcattgca
     7741 ggaaatactt accaaatcct ctgtctttta ttagccgtat taggattttg gattgtcatt
     7801 aagaatacag agttttcaat tggtaaaaac cgacgctggt ttggcggcat actcttttat
     7861 ttcggtatct tattattgtt acatgcccat ttatttggca aactccatac tggggaacca
     7921 aatatcatgg ggacgacatg ggatttgtta gccagtgata taaaacaaag tcaagtagac
     7981 aataacgttg gtggtgggat gattggagca atcctttatc attttactta cttcttgatt
     8041 gcacagcctg gaagttattt agcagcagtg ctattactgg caggaggagc attcctgttc
     8101 agtaatctgg aaggctatca gctgttaaat ggtatccaat caattggaga acgagtacag
     8161 gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga
     8221 atgaaacagc gcgcagaagc gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa
     8281 aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc agcaaaggga aaatactgct
     8341 gatgaatggc aggaaccaga aaatacagag ccggagcagc taagttttgt tccaatcgac
     8401 agttttcaag aaaatatcca tcctgcgaat ctagagaaac cagtaccaga tacaccaaaa
     8461 cagaccaata cagcagaagg tttttcagat gagttgccag aggatgacgg tacctcatta
     8521 gaatttgaga ttgaagcaga acaggaaaat caagactatg aattaccgac agttgatttg
     8581 cttgattcta taccaactgt ggatcaaagt gatgagtata aaaaaattga aaaaaatatc
     8641 ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg ccaaggtcgt caaagccagc
     8701 ttaggaccat ccgtcacaaa attcgaagtc cagccggctg taggggtaaa agtaagtaaa
     8761 attgtgaact taacagatga tatcgcgctt gctttagcgg cgaaagatgt ccggatggaa
     8821 gcaccgattc caggtaaatc actgattggt attgaagtac caaatggaaa gatcagtatg
     8881 gtttccttta gagaaatcat cgaagcacaa ccaaaccacc cagataaatt actagaagta
     8941 cctttaggac gtgatgtttc tggacgggta caaacggctg atctttcgaa gatgcctcac
     9001 ttactcgtgg ctggttcgac tggtagtggg aagtcagtag caattaatgg gattatcaca
     9061 agtatattaa tgcgtgctaa gccccatgaa gtaaaactga tgatgatcga tccgaaaatg
     9121 gttgaattga atatgtacaa cggcatacct catctgttga cgccggtcgt gacgaaccct
     9181 agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa
     9241 ttcgcggcaa ccggagtaag aaatatttct ggttacaatg aatttgttca acagaaaaac
     9301 ttggaaaacg gaacgaaaca tccaacctta ccattcatcg tagtgatcgt agatgaatta
     9361 gctgatttga tgatggtagc cagcaatgaa gttgaagatg cgattattcg tttagcgcaa
     9421 atggctcggg cagcggggat tcatatgatc ctagctactc aacgtccaag tgtggatgtt
     9481 atcacaggaa tcatcaaagc aaatgtacct tccagaatgg catttgcggt atcaagcggt
     9541 acagattctc ggacgatcat tgattctaat ggggcagaaa agctcttagg gcgaggagat
     9601 atgctattct tgccaatggg tgagaataaa ccgatccgtg tgcaaggagc attcatttct
     9661 gaccacgaag tagaacgggt cgttcaattt gttacagatc agcaagaagc tcattatgaa
     9721 gagaaaatga tgccgacaga tgaagttgaa acgacaggtg ctccagaaca gccacaagat
     9781 gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc aaacggcaag tatttccctt
     9841 ctccaaagaa gatttcggat cggttacaac cgagcagcac gtttagtaga tgaactggaa
     9901 gctcatggag ttgttggccc gtcagaagga agcaagcctc ggaaagtatt cattgagcag
     9961 gaagaaacat cagaaacgtt gccggacgat aatgacatgt aaaacttgtg tgatgaaggg
    10021 ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt
    10081 tttttccatc ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa
    10141 gttctattcg atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta
    10201 gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac
    10261 gaatgaagaa ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca
    10321 agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc
    10381 gaaacaattg atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt
    10441 cgtccaagaa gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat
    10501 gattgagact gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt
    10561 aggatgtaat atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct
    10621 gactgctgga gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa
    10681 tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta
    10741 cgacaatgta atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc
    10801 tcggcacatt acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa
    10861 cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc
    10921 gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta
    10981 tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga
    11041 tagaccagag catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata
    11101 tgtcaacttg attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa
    11161 agctgatgta ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg
    11221 taaagaacat ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa
    11281 aaaaacagtt gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag
    11341 ttttcttaat taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta
    11401 taaagctttt tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga
    11461 cagcctgctt gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc
    11521 tttcttgtgt agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg
    11581 tgatttcata aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt
    11641 ctaaatcggt cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt
    11701 tcataaacac atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca
    11761 gtacaccata actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg
    11821 taccgtaata gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa
    11881 ccaatccggt agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat
    11941 cgtcggcact atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt
    12001 ttccgatttg gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag
    12061 tagattcgac ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg
    12121 cttcggcaat aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga
    12181 gtccgtccat tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt
    12241 tcgaaccaat agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta
    12301 cagtatcgca agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt
    12361 catgctggat caactgttca atatttccat aagttgaatt acccccgtac cagagactgg
    12421 cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg
    12481 cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt
    12541 tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca
    12601 tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg
    12661 ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac
    12721 accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac
    12781 aataatgaat ttttgttcct cttagtattt ctaccga
//
LOCUS       DARBER010000074        12734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARBER010000074 DARBER010000000
VERSION     DARBER010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.075"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCK06_001859"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCK06_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1151856.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="SCK06_001860"
     CDS             complement(322..891)
                     /locus_tag="SCK06_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG1151857.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="SCK06_001861"
     CDS             1077..1979
                     /locus_tag="SCK06_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1151858.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCK06_001862"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCK06_001862"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG1151859.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCK06_001863"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCK06_001863"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG1151860.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCK06_001864"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCK06_001864"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG1151861.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="SCK06_001865"
     CDS             8547..8732
                     /locus_tag="SCK06_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG1151862.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="SCK06_001866"
     CDS             complement(8794..9990)
                     /locus_tag="SCK06_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1151863.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="SCK06_001867"
     CDS             complement(10160..11596)
                     /locus_tag="SCK06_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1151864.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="SCK06_001868"
     CDS             11889..12698
                     /locus_tag="SCK06_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1151865.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DARBER010000075        12470 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DARBER010000075 DARBER010000000
VERSION     DARBER010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.076"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..478
                     /locus_tag="SCK06_001869"
     CDS             5..478
                     /locus_tag="SCK06_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG1151866.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            487..714
                     /locus_tag="SCK06_001870"
     CDS             487..714
                     /locus_tag="SCK06_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1151867.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1350..1721
                     /locus_tag="SCK06_001871"
     CDS             1350..1721
                     /locus_tag="SCK06_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151868.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1977..2219)
                     /locus_tag="SCK06_001872"
     CDS             complement(1977..2219)
                     /locus_tag="SCK06_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1151869.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2251..3153)
                     /locus_tag="SCK06_001873"
     CDS             complement(2251..3153)
                     /locus_tag="SCK06_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1151870.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3166..3354)
                     /locus_tag="SCK06_001874"
     CDS             complement(3166..3354)
                     /locus_tag="SCK06_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1151871.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3368..3931)
                     /gene="amaP"
                     /locus_tag="SCK06_001875"
     CDS             complement(3368..3931)
                     /gene="amaP"
                     /locus_tag="SCK06_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1151872.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3969..4862)
                     /locus_tag="SCK06_001876"
     CDS             complement(3969..4862)
                     /locus_tag="SCK06_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1151873.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4940..5863)
                     /locus_tag="SCK06_001877"
     CDS             complement(4940..5863)
                     /locus_tag="SCK06_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1151874.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5912..6262)
                     /locus_tag="SCK06_001878"
     CDS             complement(5912..6262)
                     /locus_tag="SCK06_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1151875.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6295..7197)
                     /locus_tag="SCK06_001879"
     CDS             complement(6295..7197)
                     /locus_tag="SCK06_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1151876.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7190..8047)
                     /locus_tag="SCK06_001880"
     CDS             complement(7190..8047)
                     /locus_tag="SCK06_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1151877.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8381..9190
                     /locus_tag="SCK06_001881"
     CDS             8381..9190
                     /locus_tag="SCK06_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1151878.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9230..9727
                     /locus_tag="SCK06_001882"
     CDS             9230..9727
                     /locus_tag="SCK06_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1151879.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10112..10185)
                     /locus_tag="SCK06_001883"
     tRNA            complement(10112..10185)
                     /locus_tag="SCK06_001883"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10149..10151),aa:Ile,seq:cat)
     gene            10372..10695
                     /locus_tag="SCK06_001884"
     CDS             10372..10695
                     /locus_tag="SCK06_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151880.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10859..11113
                     /locus_tag="SCK06_001885"
     CDS             10859..11113
                     /locus_tag="SCK06_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151881.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11183..11428
                     /locus_tag="SCK06_001886"
     CDS             11183..11428
                     /locus_tag="SCK06_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1151882.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11504..11869
                     /locus_tag="SCK06_001887"
     CDS             11504..11869
                     /locus_tag="SCK06_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151883.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3591 a         2328 c         2128 g         4423 t
ORIGIN      
        1 aaaaatgaaa tttaaaacat tgctattaaa atttgtcgtt ttaggtataa gtgccgtatt
       61 tattttgttc ggcgtacttt tttatactca gatcgctaca agcgaaaaag gattcaattt
      121 tgattggcag accacattat ttgtttctgt catttacttt gcccttctcc tagggatcgt
      181 tattgcttat ttcttattga aactattgaa aaaaatcgaa gataaccaag ctttttccac
      241 tcaaagctta aacatactaa aaaaaatcga acgaaccatt ttgtttattg gtttcgcctt
      301 ttttggctta cttcctaaat ttatgaaagt agcggattta gacgatgctc caggtttgat
      361 gttggtagga atcgctgtcg tattcattcc ttttgcagta tacacattaa caagcgtgct
      421 ggaaaaactg cttgaacatg cgattttact taagacagat catgatttga cagtgtgagg
      481 tgcgacatgg caattatcgt taatttagat gtagtattag ctcaaaaaaa aatgagtgtg
      541 actgaattga gtgagaaagt gggcattacg atggcaaaca tctctattct aaaaaatgga
      601 aaagcaaaag ccatccgttt ttccactctc gaaaaaatct gccaagtttt ggagtgtcaa
      661 ccaggtgatt tgttgaccta taaaaatgac tcgaatacta taccagaaga ataaaataaa
      721 gtagaacaaa cagtaaataa aaaataatct gaatgataga tgtgtgagac ttttagaagt
      781 tatttacgtc tattattcag attttttatt tcgattgaat accgttttat ctataaataa
      841 gatatgtact gactcataca aagggggcct tttcttctcc atgtactatc acttttcctg
      901 atacaactgg tttcctgtta cgttgtagtt ggatctgctt ttagtataat actttattca
      961 ttccaccaga ctgatagcag tttgagcagt ctaaataatc atgtagtatt acaggccatt
     1021 ctgtttcata ccttgaagtg cttaaaactt cagctaatgg cccattgtgt taactcttac
     1081 tccattcttt tttagtaagc tcccatatat actgtaaagc cttctatata ttatgtcatg
     1141 ttgcttttta acatttacta tgtagttttg ataaaatgat ttgaataaca gtgatggaca
     1201 attctttttt atttttaaca ttattttgat aataatgatt atttatttaa aaatacatta
     1261 gataacattt gttacgacac gaaaaagctt cacaaacaaa tatcgttatt ttaaaatgag
     1321 ggcgaactca cacaattagg aggaatagta tgccatttat tgtttacttt tttctttcat
     1381 tactcgtgat ctatattcca ttaccaacat ttttcctgct cgtcaatcgt ctagcagagc
     1441 aacatgatac gattgctgct cttacgaaac tttgtttact tctatttgtt tgtctagatt
     1501 actttacgag ctattattgg ctaaaacaga caagagtaaa aaagagatat tatttcttcc
     1561 ttgtcttttt caatctcttt gagtttctga tccattgctt tttacttcat cagtatcata
     1621 cgatccattt gtggatcatc tgggttttca aagtggtcgt attattttgt atgttcacct
     1681 ttcctatctc aagaaaattt agggaggcat tgttttatta aagaagcttc tctgtatcaa
     1741 taaagacaca tttaaggaaa aacttttaaa aatctaattg ggaatttgcc cgcataaata
     1801 agaggctgtg acaaaagtct tgtcacagcc tcttattccg tataaacagt gttcaaaagc
     1861 ttgcatcttc gataatatgc ccaaccaaat gaaaaatatg agaagctatt tttgtttggc
     1921 tgttcttatt acttgatgaa gaacagcttt ttcacgacct ctttttaaaa gtctttttac
     1981 agttttcgag caaagaatga tacgattagt actaaaatga tggcaccaat caatgatggc
     2041 acgattgcca ttcctgcaat acttggtccc caagaaccta ataatgcttg accaagtgct
     2101 gatccaacta aacctgctac gacatttgca ataatcccca tcgaagaacc tttattcgtg
     2161 attgctcctg caattgctcc gataattcct ccgacgataa gtgaccaaat aaatcccatg
     2221 tttcgttcct cctaatgttt tacttcctta ttactgaacc cgtggttctg tattttcttt
     2281 tactttttgt gcgccgcttc cagctgctga ttttacttta tctgtttgat tcgaagcaaa
     2341 ttcaccggtt tttttcgctg cgtcagttac tttgtcttgc accgtttctt gatctttttc
     2401 aaattcttct tttgttttga tgtccactac gttagcgttg acttcgatca cttctaaatg
     2461 tgtcatattt tttacttctt tgccgattac ttcttgcatt tcttcaaaga ttttttcgat
     2521 atctttgcca tattccacaa tgacatccat atcaactgct acttgttttt taccgacttc
     2581 tgtttcgatc ccagccgtta cattatctgt attcacaagt tttcctgcca cattagagaa
     2641 gaaaccaccg tttgcagata ataaaccgtc tacttgttca attgcgattc cgatgatttt
     2701 ttgcacaact ttatcttcat aattcagctc accgcgcatt gaatcttcct gttcagaatc
     2761 atgctttcct tggttatttg tatgatcaga accaagtttt ccttgtccgt ttgtgtggtc
     2821 agaactaagt ttttcttgtg ctttcgcatg gtcagaagga tgtttttgct cttccacagg
     2881 tttagaagga tcgattgcat catgttgttc tggaacttta gattctggag ttcctggatg
     2941 atgaggtgca tgcttttctg catcatgttt cttctcttct actccttgtt gaacgttagg
     3001 gactactgga tctggatgtg cagatactgg tgctgttttg ttcttttcat cgttcacacc
     3061 atttgattgg tttggtgtcg ttgggtctgg atgtgcagac actggttttt ggttgttcat
     3121 tttattgtta ttatttttgt tattgttatt catggatgtt tcctcctaag atttaaaata
     3181 gtgatctaag attttcgttt gtttcagata aaaaccgata gcgacgccga tcgctgccat
     3241 gatcaagatc aataaggtct tgaaaaagcc cacggtgatc cataaaacgg ctaatatcaa
     3301 tccaagtacc ccaaagatga tagggagctt gtatgtcgaa aatagttctt tcatttttgg
     3361 tcccccccta ttctactcgt ttgcggtcat tgcttcgttt agctggttct tcaaagcctg
     3421 tgtatttcac cgatactttc actggctctt ttgttcccaa tatgtgttgt acttcatttt
     3481 cgatttcttt catcaatgtt cctgttttac caatgagtgc tgaagttcgt ttcaactccc
     3541 cttttacatt gacggcgatc ttgtttttcg ttgctttgac atttactttt ggtgaatgga
     3601 tgaattcatc ttcatgcaag tgagaacgaa ccattccttc aattgctttt ttatccaacg
     3661 tcaactcccc atcaccatgt ttcagtagaa agtttccgat tgtttttgga taaaacagga
     3721 aaacaagtaa cgtgataatt gtcaaaatag caaatgcaac ggcaagccaa aatacatatt
     3781 gttgcacgta tgaattagtg atggaaaaga atcgaaactt ctctagctga aaaggcaatg
     3841 gataggttgc acttaatagt atcgcaaaca aaagcacaga tagcaaaagt aatgttccga
     3901 tcactccgat cgtttttaca cctttattca ttttcgcacc tcctcatagg ggtgaaacga
     3961 tcaacttctt ataagttgat cgcttctcca ccagttactc cgtaaatttg ggctgtaaca
     4021 taacttgctt tattcgatgc taagaaaaca taaactggtg ctaattctcc tggctgacct
     4081 gcacgttcta gtaaactttg ctgaccaaat tcagggatcg aatcagaagg ttgtccttcg
     4141 tctagttgta atggtgtcca gattggacca ggtgctacac catttacacg gattccttta
     4201 ggtgcaagct gttttgctaa accgatcgta aagttagcga ttgaagcttt tgttgctgcg
     4261 taatctaata aatgttcgct tggattgaat gcttgaacag aagttgttgt tacgattgct
     4321 cctcctgctg ggatatgcgg aacagcttct ttcactaaag caaacatagc gatgatgttt
     4381 atcataaatg tgtctttgac ttgctcaagt ggtaactcag caatagactg acaagaaatt
     4441 tgctgtcctg cgtttagtac catcgtatct agcccgccaa gttcactcac ggctttatct
     4501 atgatttctt taggtgcttg ttcgtcacga agatcaaaag gtagtaagac aatcttacgt
     4561 cctgcatctt tcacgtattt tgctacttcc tctgcatctt tttcttctcc tgggaagaat
     4621 tgaagagcga tatctgcccc ttctctagca aatgcaatcg ctgctgcacg accgatccct
     4681 gaatctccac cagtgattag tacacggcgg ttttctagtt gattattgcc ttcataggat
     4741 tcttctccac aatcaggaac aggaatcata ttctgctgta aagctggtgt atcctgatct
     4801 tgtttaggaa atttctccgt atggtacaag tttcgcgggt cgattaattt tggttctgtc
     4861 attgctgtat tcctcctagt ttttaaattg tattgccaaa acaaatttct ttgtttgata
     4921 cctgttttaa atagattctt taatttgtat aatctttccg gtataaatgg taacctaaaa
     4981 ctactgctgc tatcaaagag ccaaagacta cgagtataaa gcctagagca gaacttttac
     5041 ccggtaatcc tcctgtattc attccccaaa tccctgcgaa caacgccgga acggaaatga
     5101 ttaatgcagc tgaatctaga tatttcatga tatgatttag attattgtcc atcatcccgt
     5161 tgaatagatc accaatactc tctaataaat ctcgatagat ctcgatcatt ttttctgttt
     5221 gtcgctgtct caaatggaca tcatagagta gctgttcatc gtctaaacgt ttgatgaaag
     5281 tttcagaatt ccataattga tccattgttt cccgttgatc tcttaaggta tgatcgatat
     5341 atacgatgtc tctttcaaga tctgcctgtt tatacagttc ttcattttcc gttgttttac
     5401 gagctgcttt atccaaagcg tcaattcgct ttttcatctc tcttagttct ttcacgtaat
     5461 gggtgtaaat atctaataat gtatacgtga tgatcttttc aaacgagttt aaatgatttc
     5521 catgctttgt cagcagccga tccacaaatt cggtttttct atcgatacaa gtaatcaaga
     5581 gattatcgga aagtacaaaa gatattggcg ttaatttttt ttcgatacga ttatctgact
     5641 gatctagatt aatcactacc aaagaaaatg gatgctctag tttcgtctca taaaggcgct
     5701 ccatccgaga aacttcttcc ggatattcgg ttcctacgaa aatatcatca ggaagctcga
     5761 attcttcgat tgcttgctca atctcttcgg gctttgcact atcaagtacg agccaattat
     5821 attttttttc atctgtttct attacattct tttcttcatc cacaaagtag tatctcaatg
     5881 tatacctcct ttttggactt tctcgaaact attagctgaa gaagcagttc tttaatttga
     5941 tattataatc gagtactgcg atttcatttt ctaatgaaaa gctgtcaagc tgtttgttga
     6001 agtccatgtt caatcgttta ccgaaataat tttgctctaa tttagatgta tagatgtcca
     6061 ataatggatg ctcgatttta gtcgcatatt ctccaaattc cgaaacaaaa ggaatgattt
     6121 gaaaaaagct gccttttgca aaagcacctt tgtcccttag aaatggatcg gaatcattga
     6181 taagcgcgag tgcgattttt gtattttccc caaccttttc aattaacgtc tctactgcat
     6241 ttttagacat tgtaatttcc attcctactc cccctcttca gttgttgtct tctattattt
     6301 aacaactaca tctgtttttg gttgaacttt tgcatatttt ggatatttag ggaatttagc
     6361 tacacaacgg aaagccgtca tccctgcata tttttcgtaa tatttcttcg ttggcccttc
     6421 aaaatccttg ctccacggtt tcacatgtcc tgaaaaatgg ataatgtaag gattgttgcg
     6481 agtttcttcg tattcacgtt cacctgctgc tgttggatgt ttttttgctt ttgccatgat
     6541 atagccttgt gcattccaac gcggatgaag tggtaaccaa cggtcatgta agatagcatt
     6601 taatgcatct tggtcatgga atcgtaattt ttctggatta tgttcgatga aatctaatac
     6661 tttttgcgta atattttcat ctaaccattt tttcacattg atcagcatca aacctgagtt
     6721 gaaataacgc attgatttag ctgaaatttt cattttttcc agtcgttgat ggaacccagc
     6781 gtcttcaaca gctgctacga ttgaatcacc gaagtcaaga cgccataatt tagagatatc
     6841 ttgtaaagcg atcatgtcgc aatccatata taagatacgt tcaacttctg tgccgcggaa
     6901 caattccgga atcgcaatac gataataagc tgtttctgga atacgatcgc tgattaccac
     6961 atttgtaaag aattctttat tgattttcaa gaattccaca tctgagttca ttcgtgcgtt
     7021 tttcactgag aagcgtaaaa gctgtttact ttcaaaatcg atatcatcgt caatcacata
     7081 gaaccgaaca aatttagacg ggttgcaatt atctaatact gagacaaata atgcagctaa
     7141 atgtggtacg aattttgtgt tacaacttgc gactactgct atttcttttt tattcattga
     7201 tctcgcctac tttcgttaga gtactgtgtg ctaatttttt taagtataaa ttggtatacg
     7261 gatgatcttt caacgtattc caaggtttat catgtcctgt aaagtgaacg atcgaaggtt
     7321 tttcatttcc ttctttatac tgacgttcat atttttcatt tggtgctgga tgacgttcaa
     7381 aaatcagcga agtctgcatg ttccattttg gatgaagttg ttcccaatct tcataaagta
     7441 cagcattcaa agcatcttgg tcgtgataga tgattcgatc gccattttca ctgaggtaat
     7501 ggatagtttt ttctgtaatt tcttttttat tccattgatc aatgtcaatc accatcactc
     7561 ctgaattgaa gtacaagtca tcggaatcga ttcctaatct ttccaaagct ttcacttggc
     7621 ctggatcgat aatagcgccg attgtttttc catttaacgg ctcatcatat agttttacga
     7681 tatcgtctag tactaacaca tctgaatcta agtaaaggac tttcttataa tcctctttag
     7741 ctaataagtt tggtaaagaa attcttaagt aagcggtagt tgtgatatgg tcactgacta
     7801 ggaagtcttc gtagatatct ttttccactg tcaggaattg gatacttgca ttacttgaat
     7861 atttatttac cgtttcttcc aatccttctt tactatattc tgatagacca tcgtcaatca
     7921 cataaaattt tatgcgtctt gttttattgc aattttctaa tgcagttgcg atcatcacgc
     7981 tcaagtaagg agcataattt tcgtctgaag cagttaccac aggaactacg ccgtatctct
     8041 tctccatcaa aatcactcct ttttctaatc tttttctaac ctgttctaca tctagaatat
     8101 tcctcatttg gttttaaagc aaaatatatg ctttgttttc attgaatatt ttaaaatttt
     8161 tttatcacaa aacgcatcgg taagcgctaa ctattcgttt ttcatggctt tttattcaaa
     8221 ggtattgacc aaaaactact atatatagcc acctttttct taagaatgtt ttttgaaaaa
     8281 attcataatg acaaagcaag ttagtttcat ttttttaaat tctcatttaa tcttaaggta
     8341 acaggaaaat agatatgtaa aaagaaagta ggaagataaa atgaaactag cagcgattga
     8401 tttagacggt accttgcttg atcgaaatgg aaagatacca gaagaaaata tccaagcact
     8461 tcggaaattt gatgaaaatg gcggaatcgt aaccgtcgct accggaagga acagcatttc
     8521 tgctaaagat attttttccg aattaggaat cggtggctat ttgatttctt ccaatggttc
     8581 actgattgct gaaatgaagg atggaaaaat ggcgaaagtt ctcagacgtt caaaaataga
     8641 tagcgataca ctgagaaaag ccttcttcta tgctcaagaa gcgaaaatat ccatcattgc
     8701 tagccgagaa acccaagatg atcagctgac attcgatgaa tcaaatctag tcaaagatga
     8761 cccttattat gaacatttca atttacagaa ccattctttt gatcaaattt taaatatgtt
     8821 ggaagatcct tctcttagct atcttaaatt agcattaaca gataagagtg aagaaaaact
     8881 acagaagcta caaagagatc tgcaagcaga tggtatccaa tcagtattct ctgatccgca
     8941 tttcttagaa atcacgccta caggtatcac taaagctcat tcacttgttt ttctggcgga
     9001 ttaccttgga atcgaatcga gtgacatcat ggcttttggc gatcaagaaa acgacattgc
     9061 tatgttgaat tacagcgggt tgagtattgc gatggggaat gcacagcgac aagtaaaaga
     9121 aattgcaaac caagtaacac tgacgaatga agaagctggt gttgcttata ttttaaaaaa
     9181 cttctattaa aaagaataaa atgacgatat gaatgtagga ggaaagaaaa tgttagttat
     9241 tattgatatg caaaatcata ttcttgatcc aaacagcgaa ttttatattc cagagtcaga
     9301 tcaattggca gaacgtattg ctcaaagatt agctaaagca cgccaaaata atgaatatat
     9361 tctcttcaca cgggatatac cagttgaact gaaggacaaa gaagaagaac gagaagattt
     9421 gcaattgatc cctcttcttt ctccacaaga aaatgaacga gtgattaaaa agtattactt
     9481 tacgattcca cctgaaactt tagctgaaat ccaggaaact ttatttgaaa gcaaagaaga
     9541 acaaaaagag atcgaagtag tcgggattga aacaaacttg tgtgtactct ctaatctgat
     9601 aggcctgcag agtgcttttc ctgaagcaga cttttttgtt gatccaatgt tagtctccag
     9661 tagaaaacac ggagaatccg ctttagagct gttgaaagat ttcaacgtct ctatcgtcga
     9721 atcataaaaa tatctatctt catttacgat atttaacaca caataaaaaa agcacgcaga
     9781 actgttttag tcctacgtgc tttttctatc aaagtagatt ggctttttta tgacacagag
     9841 tgaaacggca ggctcacgac ctctttctta aatggtgttc aatcagctga ccctcggtat
     9901 tagcttaaca aacggcaaaa tatgaggaac tatttttccg tttgttaagc taatactcag
     9961 gtcaaaacgc tgatttcaca acctcttcct taaacggtat tcaaaagctt gcatctgcag
    10021 taataagaac agccaagtaa aaacataaga agctgttttt acttggctgt tcttattact
    10081 tgatgcaggg cagctttttc acaacctctt atgtaccttg taggactcga acctacgacc
    10141 ggacggttat gagccgtctg ctctaaccaa ctgagctaaa ggtacatcat aaataaaatt
    10201 tatttttgtt acttttcaac tatagcacga atgacaaact ctcacaagtt tcaaaatata
    10261 agtaaaataa tattgttatt cttatttctt ttacttgtgt cataactatt ttgttacttt
    10321 aatttttatt tgcttatttt agaaaaaatg ttattctttt atttattctc aatgttagga
    10381 gtgattattt tgccatttat tgcgatttta ttcgatttag ttgcggcagc agcttatttt
    10441 ctccaattaa attatcaaac ctcaggaatt ctgtttattg gaatgatttt tcaaggtgtg
    10501 attacccttt tgcttcttgt catgatgctc agctataaag gaaaaagata tgcccgtatt
    10561 caaacaaaag tatttgttaa atatgtttcc attcgctatg ggatcatcat tctttccttt
    10621 ttagtgaatg cgatcgtcct ctttttatat gtattgaatt acttagatat caatccattg
    10681 attttttctc gatagataag acacattcag cggaacagtt cctgcttatc agcagaaaac
    10741 tgttctgctt tttcattttt cattgatcta ttcttctttt attttcctag catttcattt
    10801 gtgatgtatg caataacatg ttatctttta ttatatgaat cgttgaggag tccgttacat
    10861 gattacgttg gtgagagttt tgttttgggt gcctgcggtc gcactggtcg caagtattgt
    10921 ttatcttatg aattggaaca aagaacggtt ttatttagct attttgactc ttccggctat
    10981 ctactttatg tggaaagttt ttaattacaa ctactttgaa cccgatagtg tttttataga
    11041 agaattatca ggactggttt tgtctttatt gatcgtcatt ctttatttga tacgtttaaa
    11101 taaaaaacat tgagtactat tgattgcatg tgtagctttc tatagtcatt ataactattc
    11161 tactataatg aaaggtgatt aaatgagtat cgaagaagct attttactgg atactttacg
    11221 ttgtcgtctg aatttcttaa gaactggccg atctttatct caagtagctg tttctagcgc
    11281 gttaagtatt cctttattca aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat
    11341 cattgaatta atctatatcg ctgactttta cgatatttcc gttgattacc taattggtcg
    11401 ctcagaaaca gcaagcaaac tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt
    11461 taacgtctaa ggttaacaat ctatatataa aggagacatt tctatgcgat tatggcatga
    11521 aaaattgatt tcaaagcttc ctcgtcaaca gcttttagga caacatcggg aatgctgtgc
    11581 attaagagga aatggctggg gaaagaaaca tgctactgtg gattatgttt ttcactattc
    11641 tccttataga ttattccaat atcatcagct tgtcatggat gaaatggaaa aacgtggtta
    11701 cagcccagca ccagagtgga aaaatcctat gtatcgtggc aaatcatgcg aagcattttt
    11761 tgatttacct actgtagagc gcaacgtgcc gatttatccg gaacacgatg aagtttatct
    11821 agcagaatgt ttagcaaatt tggagcaaaa aggaatttat cttacctaat ataactagaa
    11881 aagccttcta tctcctctta cttcattaaa aagctttaac catcttagat caagctaaaa
    11941 tgtcgtaagt tttagggtta tcttcatcct cacgtgcaaa actaaaagcg tactctagtt
    12001 ttggttcatc gccaacttcc ctagacataa atgaacatct cctgacgtgg tttaaaagta
    12061 ccgcatcttc gacgtctgta tacgatgcac agcacagatt gcttcttttg atctttcttt
    12121 tgatttttct gttgcttctt ctacaccttc ttcgattact acatcgatgc ttacaattgc
    12181 ttctgcctaa tattcccatg catcgtcgat ttaagcctct gtagtcactg tgatcgtttc
    12241 cacattttaa ctcctctgtc gtggcttcag ttgattttgt gatcagttca gttgctaagc
    12301 cagagtcaga acaacctgcc attgctagcg ttaatattga tagtaaaaat agttttttta
    12361 tcataaggtt ccccccctga aaatagacta aaataataac gctttctttt tttgttttca
    12421 gacgaaaatt aataatttct aaatattctt taaaaaagct tataactatt
//
LOCUS       DARBER010000076        12351 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARBER010000076 DARBER010000000
VERSION     DARBER010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.077"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCK06_001888"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCK06_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG1151884.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="SCK06_001889"
     CDS             complement(1195..3036)
                     /locus_tag="SCK06_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG1151885.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="SCK06_001890"
     CDS             complement(3218..5533)
                     /locus_tag="SCK06_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1151886.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="SCK06_001891"
     CDS             complement(5718..6578)
                     /locus_tag="SCK06_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG1151887.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="SCK06_001892"
     CDS             complement(6637..7158)
                     /locus_tag="SCK06_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG1151888.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="SCK06_001893"
     CDS             7436..8197
                     /locus_tag="SCK06_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1151889.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="SCK06_001894"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="SCK06_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1151890.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCK06_001895"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCK06_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1151891.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         3485 a         2619 c         2187 g         4060 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta a
//
LOCUS       DARBER010000077        12205 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARBER010000077 DARBER010000000
VERSION     DARBER010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.078"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..216)
                     /locus_tag="SCK06_001896"
     CDS             complement(37..216)
                     /locus_tag="SCK06_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151892.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="SCK06_001897"
     CDS             complement(597..1304)
                     /locus_tag="SCK06_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151893.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="SCK06_001898"
     CDS             complement(1297..1734)
                     /locus_tag="SCK06_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151894.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="SCK06_001899"
     CDS             complement(1749..2000)
                     /locus_tag="SCK06_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151895.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="SCK06_001900"
     CDS             complement(2000..2206)
                     /locus_tag="SCK06_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG1151896.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="SCK06_001901"
     CDS             complement(2643..2903)
                     /locus_tag="SCK06_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151897.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="SCK06_001902"
     CDS             2978..3181
                     /locus_tag="SCK06_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG1151898.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SCK06_001903"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SCK06_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1151899.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="SCK06_001904"
     CDS             complement(3810..4709)
                     /locus_tag="SCK06_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG1151900.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="SCK06_001905"
     CDS             complement(4722..5315)
                     /locus_tag="SCK06_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG1151901.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="SCK06_001906"
     CDS             complement(5391..5540)
                     /locus_tag="SCK06_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151902.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="SCK06_001907"
     CDS             complement(5556..7760)
                     /locus_tag="SCK06_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG1151903.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="SCK06_001908"
     CDS             complement(7771..9231)
                     /locus_tag="SCK06_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG1151904.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="SCK06_001909"
     CDS             complement(9292..11664)
                     /locus_tag="SCK06_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151905.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFSNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            12074..>12205
                     /locus_tag="SCK06_001910"
     CDS             12074..>12205
                     /locus_tag="SCK06_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1151906.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         3041 a         2531 c         2052 g         4581 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcgaaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa ggatccaacg
    11821 ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca
    11881 ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt
    11941 ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata
    12001 cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg
    12061 aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt
    12121 ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga
    12181 catcgaaggt gcttcattca tacga
//
LOCUS       DARBER010000078        12147 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DARBER010000078 DARBER010000000
VERSION     DARBER010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.079"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(29..586)
                     /gene="frr"
                     /locus_tag="SCK06_001911"
     CDS             complement(29..586)
                     /gene="frr"
                     /locus_tag="SCK06_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG1151907.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(589..1311)
                     /locus_tag="SCK06_001912"
     CDS             complement(589..1311)
                     /locus_tag="SCK06_001912"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG1151908.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1447..2328)
                     /locus_tag="SCK06_001913"
     CDS             complement(1447..2328)
                     /locus_tag="SCK06_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG1151909.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2427..3209)
                     /gene="rpsB"
                     /locus_tag="SCK06_001914"
     CDS             complement(2427..3209)
                     /gene="rpsB"
                     /locus_tag="SCK06_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG1151910.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3568..4047
                     /locus_tag="SCK06_001915"
     CDS             3568..4047
                     /locus_tag="SCK06_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG1151911.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4264..5355
                     /gene="phnW"
                     /locus_tag="SCK06_001916"
     CDS             4264..5355
                     /gene="phnW"
                     /locus_tag="SCK06_001916"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG1151912.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5348..5476
                     /locus_tag="SCK06_001917"
     CDS             5348..5476
                     /locus_tag="SCK06_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1151913.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5480..6025
                     /locus_tag="SCK06_001918"
     CDS             5480..6025
                     /locus_tag="SCK06_001918"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1151914.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6479..8170)
                     /locus_tag="SCK06_001919"
     CDS             complement(6479..8170)
                     /locus_tag="SCK06_001919"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG1151915.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8590..9537)
                     /gene="arcC"
                     /locus_tag="SCK06_001920"
     CDS             complement(8590..9537)
                     /gene="arcC"
                     /locus_tag="SCK06_001920"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG1151916.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9652..10671)
                     /gene="argF"
                     /locus_tag="SCK06_001921"
     CDS             complement(9652..10671)
                     /gene="argF"
                     /locus_tag="SCK06_001921"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG1151917.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10762..11991)
                     /gene="arcA"
                     /locus_tag="SCK06_001922"
     CDS             complement(10762..11991)
                     /gene="arcA"
                     /locus_tag="SCK06_001922"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG1151918.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3314 a         2526 c         2196 g         4111 t
ORIGIN      
        1 gatccgcccc tctgttttta tgattttatt aaacttccaa taattctttt tctttatcag
       61 cagcgatctt atctacttct ttgatgctgt tatctgtcaa tgtttgtacg tctttttcta
      121 atccgcgcaa atcatcttcg gtaatatcac catttttttg ttgtttttta tattcatcga
      181 tcgcatcacg acggatgtta cgaatcgcga tttttgcatt ttctgcttct tttttaacat
      241 ctttagctaa ttcttttctg cgttcttctg tcagttgcgg gataactaag cgaatgacat
      301 ttccatcatt ggttggactg attccgatat cacttgccat aatagctttt tcaacatctt
      361 gaagaatgct cttgtcaaaa ggtgtgatca ttaaaacacg tgcttctgga atttggattg
      421 atgccatttg gttcaatggt gttggcgcac cgtagtagtt cacagtaata cggtccaaaa
      481 ggctagcgtt cgcacggcct gcgcggattt gcccaagttc acgttgaaga ttttgtgctg
      541 cttttttcat tttttctttt gcttctgtca taattgcatc tgccattttt atttccccct
      601 tacagttgtt ccgatatttt cgcctaagat tgcacggcga atatttccgt gttcatttaa
      661 attgaagaca agcagtggaa tatcattatc catgcttagt gaacttgcag ttgaatccat
      721 gacttgtaat cctttggcaa taacttccag atgagtcagt tcttcaaatt ttactgcatt
      781 tgcatctact ttcggatcag cagaataaac accgtctaca ttgtttttag ccatcaggat
      841 aacatctgcg ccgatttctg ccgcgcgtaa tgctgctgtt gtgtctgtcg agaagtacgg
      901 gttgccggta ccgcctgcaa agataacgat ccgtcctttt tctaaatgac gttctgcttt
      961 acggcgaata tatggctctg cgatttgacg catttcaatc gaagtttgca cgcgtgtcgg
     1021 tacacccacg ttttccaacg tatcctgtaa agctaaggca ttcataactg tagctaacat
     1081 acccatgtaa tcagcttgag cacgttccat gcccatttgt gctccgattt gtccgcgcca
     1141 gatatttccg ccgcccacaa cgattgccat ttcaacgccc agctcgtgaa cttctttgat
     1201 ttcttggatg atttctttga ttactggtgg tttgattcca aatcctgcgt ccccagctaa
     1261 agcttctcca ctcaatttta agactacacg ctgatatttc ggtgtgacca tgttcattcc
     1321 tccaatattt tctattgcca ttttaccata cttagatgat ttcgacactc taagactggt
     1381 ttttcttgtt ttgcttaaaa aaagggagcg cacaaaccgt gcgttcccgt ctgttattca
     1441 acagaattat tttttcactt ggctcataac ttcttctaca aagttatctt cgcgtttttc
     1501 gataccttcg ccaacttcga aacgtacgaa tgttttaaca gttgcgcctt ttgatgcaac
     1561 gtatttttca acagtcatat caggatcttt aacgaatggt tggtccacca aagcgatttc
     1621 tgctttgaat ttcttcaagc gtccttctac cattttttca acgatattcg ctggtttgcc
     1681 ttcgttcaat gcttgttctg taagaacagt tttttcatgt tctaattcag cttctggaat
     1741 ttgtgattcg tttacataac gaggattgat agctgctacg tgcatagcta cgtctcttgc
     1801 aactgtttcg tcagttgttc catctagaac agtcaatacg gcaatacgtc cgcccatatg
     1861 caagtatcca ccaaatgcag cattgtcgtc tttttctaca acttcaaagc gacggaagct
     1921 gattttttct ccgataactt gagttgcttc gatcaagtca gattcaattg tacctttatc
     1981 tgttttgatt ttcattgcag cttccatgtc tgctggtttg ttttcagcaa caagttctgc
     2041 aatttctttt actaaatctt ggaacatttc gtttttagaa acaaagtctg tttctgagtt
     2101 tacttcaacg attgctgcag tgttaccttt cacagcaact gaagccaatc cttcagctgc
     2161 aatgcggtca tttttcttcg cagcctttgc cattcctttt tcacgtaaaa gatcaactgc
     2221 tttttccatg tcgccttcta cttcaactaa tgcttttttc gcatccatca ttcctacgcc
     2281 agtcatttcg cgtagttctt ttaccatttt agctgtaacg tctgccatgt ttaactcctc
     2341 ctgtgtgttt ttctttttaa aaaaagctgc ttcaaaggag agtgaggctt gtcgcccagc
     2401 ctttgaaaca gctccatcat tcaaaattat tctgctgaag aattgtcgcc ttcaacaaca
     2461 tctacgattt cttcgattga tgttgctgtg ttttcttctg tgaaatcttc ttcaactact
     2521 tgatcttcac cttggtttcc ttcgatgaaa gcatcagcca tttttgaagt aatcaattta
     2581 acggcacgaa ttgcatcgtc gtttgaaggg attactacgt cgatttcatc tggatcgcag
     2641 tttgtatcaa ccatagctac gattgggata tttaatttgt gtgcttcttg aacagcaata
     2701 cgttctttac gtgggtcaac gatgtacatt acatctggga ttcttggcat atcagcgata
     2761 ccgcctaaga atttttcaag acgttcacgt tctttgttca aaccagcaac ttctttttta
     2821 ggtagaactt caaaagttcc atcttcttcc attgcattga tttgtttcaa acggctgata
     2881 cgtttttgga tagtatccca gtttgtcaat gttccgccta accaacgatg gttaacgaag
     2941 tattgtcctg cacgagttgc ttcttctttg attgcttctt gtgcttgttt ttttgtacct
     3001 acgaataatg ctacgccgcc ttcttcagca acgctcttca tgtaatcata agctgcatct
     3061 actaatttaa ctgttttttg caagtcaatg atgtagattc cgtttctttc tgtgaagata
     3121 tatttcttca tttttggatt ccagcgacga gtttggtgac caaagtgtac gccggcttct
     3181 agtaattgtt tcatagaaat tactgccatg tttgtttcct ccgatttggt tttatttttc
     3241 ctcttcttat cttctacctt gccggcaaac tttttcaagc acctaccgac aatccaataa
     3301 gaatgtggat ttgctgatgc ataaagcacc ctcagctatt atacagacaa aagatccgta
     3361 tcgcaagagc aaatatactt ttctgctcaa cattcaatag tttacctgaa agataaaaag
     3421 aattcaagaa aaacagtaga tttctctttg tttctttatt atcctttttc cataaaataa
     3481 ttttaggtat aaaagacgaa tattattggg caatccttct tttttttgtt atgatacatg
     3541 tgaaatcttt cgtaaaggag aaagaaaatg cgcaaagctc aaagacattt gctgattaaa
     3601 caagtcattg aagaattccc gatccgtacg caagaagaac tattaaacaa acttgggcat
     3661 tatggcgtta ccgccacgca ggcaacgatt tctcgagata tccgtgatct aaaaatcgtg
     3721 aaggcacctg atgaaaatgg cgtttcccgc ttcgtattgt ttcgcgaaaa aggaactcct
     3781 gagactagga ctgaggaaga ggatcggctg gttcaaatga ttgaagacat tgtattgaaa
     3841 gtcgaacgag tccacttttt gacgatcgtc aataccttgc cagacaatgc tcatttattt
     3901 gcatctgttt tagatgagat caaacctccc tctattgtca gtacaattgc tggattcgat
     3961 acgatcatgg tcatctcgga aaatgaggag gacgcaaaac gagtagaaga atttcttcac
     4021 catccaactg aagtatcact tttttaatca gagcacgtat tcctaatatg gaatgcgtgt
     4081 ttttttattt tcaatatttt ccttattgaa taaaccttta cactctttac aaaaatacat
     4141 cattttttat tcaaattttt caacaccttg attgaatcac aataatcatc atttatattg
     4201 gatgttaaca agagcagctt cttgctcttt gtgtatttca aaaaagaaat gaaaggaaat
     4261 catatgtata aattattaac ttctggacca ttgacgactt ctgaaacagt gaaaaatgct
     4321 atgctgatcg atcattgtac ttgggatcaa gaatacaaag aaattacaca atggataaga
     4381 aaaaaattat tggatcttgc tcaagtatcg gaaggaatat acacaacggt cttaatgcaa
     4441 ggtagcagct ccttcggggt agaatcggta ttaagttctg tgatcaggcc agaagatact
     4501 gtactgattt gttcaaacgg cgcatataga caacgaatga tcaacatgtg cgaatgtctg
     4561 aaactgaact ataccgttta tgaggtatcg gaacatcaaa tcctccaagc tcatgtgatt
     4621 acacagctga ttgaagagaa tccgtctatc tccgcactag caatggtcca tagcgagact
     4681 acttccggta ttttgaataa tttggaagag attgctgctg tcacacaaga aagagatctt
     4741 ttgtttatag tggacgcgat gtcaagcttt ggtgggatac cgatttgtgt agccgaacta
     4801 gggattgatt ttttgatttc cagctcaaac aaatgtattc aaggtgttcc aggcttttct
     4861 tttgtacttg ctaataaagc ggaactggaa aaacgaaaaa atgttagccg aagtttgtca
     4921 cttgatctat atgaccagta ccgcgtgatg gaaaacgaga atggcaaatg gcgtttcact
     4981 tcccctaccc atacggtttt ggcttttcaa caagcgctga aggaactaga ggcagaagga
     5041 ggaatcgaaa ctcgatattc acgttatctc gccaataatc atgtgctacg acaaaaaatg
     5101 acagagatgg ggttccagcc tttcattcaa gaatatcagg ggccattcat cacctctttt
     5161 ctttacccta aggactggcg atttgatttt aatcattttt atcagtattt aaagcaaaat
     5221 ggatttgtga tctatcccgg gaaaatcagc gatcatgaat gttttcgtat cggaaatatt
     5281 ggcgagatct atccggaaga tattttggaa ctgacagcaa ttatcaaaga ctataaggag
     5341 aacgttaatg attaaaactg taatttttga ttgggcagga acaactgtgg actttggctg
     5401 tatggctcct gtccacgctt ttagaaatgc ttttctagaa aaaggaatcc aactgactga
     5461 caaagaaatc cgctaaccga tgggaaaatt aaaatgggat catatccaac agcttctttc
     5521 cctcccttct gtcaaaaaac aatggatagc tatttatgga caactacctc aggaaaaaga
     5581 ggtggaggaa ttgtatcaac aatttgaaca ttatctgttt aaagatttgg tcaagcattc
     5641 taccttgaaa cccgacacat tggaaacgat cgaacggttg agaaacgctg ggatcaatat
     5701 tggttccacg aatggattta atgctcctat gatgacaatc gtagcagaaa ccgcaaaaaa
     5761 agaaaggtat tcaccagact tcatggcaac ttttgaagac gtcgaagggt atggtcgacc
     5821 gtttccttat atgatccata aaaacatgca gtacttcaaa aacaagtcgg ttgatgaagt
     5881 aatcaaagtc ggagatacgg tctcagatat ccaggaaggg aaaaatgccg gagtgttgac
     5941 agtaggtgta attgaaggta gttcgctaat ggggatttct tatgatgagt accaaaaatt
     6001 agagatgggg aaaaagatcg tttaaaacag tctgttgctc ttagattcca acaagccggc
     6061 gctgatattt gtgtaaacaa tttaaaagag ttaacagaat acattttgta cgctgacatg
     6121 aatccagaat gacttttata caaaataaga ggttgtgaaa aagctgtcct gtatcaagta
     6181 ataaaaacag ccaagcaaaa atagctcctc atatttttcg tttggttagg cttattaccg
     6241 cagatgcaag cttttgaaca ccgtttattc gaaaaaaaaa agggagctgt gacacgactt
     6301 ttgtcacagt ccccttattt gcaaacaaac agtgtttaag agacaacctc tccactaaac
     6361 ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac gataaaattt gaaaagctat
     6421 tttctctttt gtttctctaa tactcggatc agaacgctga tttcacattc tctttttctt
     6481 acatcttatc tggtgcatga agtcctagca aacgaaggtc ttctttcaat aatacagtca
     6541 ctgcataaac tagagctaag cgtgcttctt tttgttcatc atcagctaaa atcttcgtat
     6601 gagcatagta tttgttgaat gcttgtgcta atttgattgc atgtttcgca atcactgacg
     6661 gttcgtattt ttccccagca gacaagaccg tttcagggta tttttgaact aatttaacca
     6721 cttcccagct ggtatcgtca ttcaatgcat agtctgctgt ttctgaaggg acaaatcctg
     6781 ctttttccaa caggctgact gcacgagcat gtgtatattg cacataaggt ccagtttctc
     6841 cttcaaatcg aacaacttct tccaggttaa agtcaaatgt attcagtcga tcgtttttca
     6901 aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc tacagcatct ttgttttcca
     6961 aatcagggtt tttctcgctg atttgttctt tagcagattg gattgcttca tttaatactt
     7021 cttctaataa gacgatcttt cctttacgag ttgataattt tttaccacct tgcgtgatca
     7081 aaccaaatgg aatatggtgc atgtcgtcag accagtcaaa gcccatttcc ttcaagacag
     7141 ctttcaactg tttaaaatga tagctttgtt cattccctac tacatataga gattgtttga
     7201 agtcgtaagt acgtttacgg tacaatgcag cagctaagtc acgtgtaatg tatagtgtcg
     7261 caccgtctga cttcttaatc aacgctggat tcaggtcata agcagatagg tcaacgatct
     7321 ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa aagtttgacg atttcatcca
     7381 tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa acgtacttcc agcagatcat
     7441 agattttatt gaattctttc atcgattcat cacggaacca ttgccataat tgaatagcct
     7501 ctttatcgcc ttcttccaga cgtttgaacc aagcacgagc ttcttcattt aattctggtt
     7561 ctgtttcagc tacttcatgg aattggacgt ataaacgaag caattcattg attggttctg
     7621 ctttaacagc ttcttctgta ccccattttt tgtaagcgac gatcagtttt ccaaactgtg
     7681 ttccccagtc acctaaatgg ttgatgcgga ttggctgata accgattttt tccataatga
     7741 aaccgatcga attaccgata actgtcgaac gcaaatgtcc cattgagatt ggtttggcaa
     7801 tattaggaga agacatgtcg attggtactg ttccttgatt cccgatgttg ctatccccat
     7861 aatgttcttt ttctttcact actgtttgca aaactttttt gctgataagt tctttattca
     7921 tgaaaaagtt caagtaaggt ccgacaactt cgattttttc aaagttggca gaatcgattt
     7981 tttctgctaa atctgcagcg atttgctgtg gcgcttttcg gtaaactttt gctaatgaaa
     8041 aggctggaaa tgcaacatct ccgtgttcag ctgattttgg attttctagt aattgctcga
     8101 cttgttccag tgttaaatca tctttgacag cgtcatagac agcttttgcg acaagatctt
     8161 tattattcat ctttattcct cctaaagtgt tcccttgatt gttaaacaaa aaaaatctct
     8221 agcgacggca tacttagtat gccatcacta gagacgagat ttcccgcggt accactccaa
     8281 ttgtcctaaa aaaggaccag ctccatctga tatcggtaga cgaccgtctg tttaaaacag
     8341 aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc ttccaccact tccgaattcg
     8401 ctagccaaga atgatacaag tactctcttg ttcaacaaat cttagtctta ttaaactttc
     8461 ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt cataaaaaaa caatccagat
     8521 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
     8581 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
     8641 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
     8701 tgcttctact ttcggaagca tgcttcctgg agcgaattgc ttttcatcaa tgtattgttt
     8761 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
     8821 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
     8881 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
     8941 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
     9001 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
     9061 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
     9121 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
     9181 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
     9241 cagtacttcg cccatggcgt tttgtaacca gtaaccgata gatccttctg tcattgcaac
     9301 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
     9361 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
     9421 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
     9481 atccgtagac aagatggcat tgccccctaa agcgacaact acttttcgtt tggccatttc
     9541 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
     9601 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
     9661 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
     9721 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
     9781 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
     9841 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
     9901 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
     9961 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    10021 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    10081 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    10141 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    10201 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    10261 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    10321 ctctaccatt ttttgactga atccacggaa ttcgattccg tcaaacatac gtcctagtac
    10381 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    10441 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    10501 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    10561 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    10621 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    10681 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    10741 agactgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    10801 gcgtggacca ccacgacctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    10861 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    10921 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    10981 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    11041 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    11101 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    11161 cattgtaaat acggtatcca aatgcatcat tgcacggttg ttcggaattt tgattgccaa
    11221 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    11281 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    11341 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    11401 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    11461 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    11521 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcata
    11581 tagactgcta gaacgtactt cgatatcttt tgtacgaacc ccagccatga ttttatctac
    11641 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    11701 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    11761 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    11821 cttcgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    11881 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc gtttcagtaa
    11941 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    12001 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    12061 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    12121 ttttctattt ttaagcgtaa tttattc
//
LOCUS       DARBER010000079        12052 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARBER010000079 DARBER010000000
VERSION     DARBER010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.080"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(139..726)
                     /locus_tag="SCK06_001923"
     CDS             complement(139..726)
                     /locus_tag="SCK06_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151919.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            901..2223
                     /locus_tag="SCK06_001924"
     CDS             901..2223
                     /locus_tag="SCK06_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1151920.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2378..3154
                     /locus_tag="SCK06_001925"
     CDS             2378..3154
                     /locus_tag="SCK06_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1151921.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3147..3716
                     /gene="rnmV"
                     /locus_tag="SCK06_001926"
     CDS             3147..3716
                     /gene="rnmV"
                     /locus_tag="SCK06_001926"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG1151922.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3709..4593
                     /gene="rsmA"
                     /locus_tag="SCK06_001927"
     CDS             3709..4593
                     /gene="rsmA"
                     /locus_tag="SCK06_001927"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG1151923.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5044..5673
                     /locus_tag="SCK06_001928"
     CDS             5044..5673
                     /locus_tag="SCK06_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151924.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5797..6906)
                     /gene="ugpC"
                     /locus_tag="SCK06_001929"
     CDS             complement(5797..6906)
                     /gene="ugpC"
                     /locus_tag="SCK06_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG1151925.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7115..7537
                     /gene="mgsA"
                     /locus_tag="SCK06_001930"
     CDS             7115..7537
                     /gene="mgsA"
                     /locus_tag="SCK06_001930"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG1151926.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7860..8396
                     /locus_tag="SCK06_001931"
     CDS             7860..8396
                     /locus_tag="SCK06_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1151927.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8436..10199)
                     /locus_tag="SCK06_001932"
     CDS             complement(8436..10199)
                     /locus_tag="SCK06_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151928.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10202..11917)
                     /locus_tag="SCK06_001933"
     CDS             complement(10202..11917)
                     /locus_tag="SCK06_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151929.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3755 a         2223 c         2460 g         3614 t
ORIGIN      
        1 ttgatgtgtt aattataagt tctatcactt tatgttcaat tagtttgtgc tcttatatgt
       61 gtgattgaaa atatagataa aaaaacaatc atcggctttg acttcgatga ttgttttctt
      121 tattttgtaa aaatcctatc acttgatttt atggtaacct tttatttttg tcttcaaacg
      181 gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt
      241 acgtccgata ccataatgtt gatagagctt tgtcaacatc acgtgtaaca agatcaaccc
      301 taaaagcaag gagccagcta taaaccaatg attatcccaa tgcatgactt cactaagtgc
      361 catcactcca aaaggataga aataaaaata gatcccgact aaggtcatcc gccagttctt
      421 tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg
      481 aaaaagccca accaaaaagg aatacaacat cccccacaag atcatcacag gacctttgat
      541 caatgcaacg attgcgatga acaaagtcag caatattagt ttttgtgata acgttgcaac
      601 agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt
      661 acgggaaaaa tcgacccggt tggataaata atctgctaat ttcggataag aacgcttaaa
      721 atccataaac tgttcctcct tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag
      781 aaatacatcc gtcttaagag ggagtttttc actgaaagtg gagaaaaaag aatggtaaat
      841 taaaatattc tcagttatac tgaaaattat accaaatttc aaagaagaaa gaggatggga
      901 atgaaaggca aactaaaacg agaaatcaat ctgtttggcg cattagctac cgttatggga
      961 accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg ttactgctag tactcagtct
     1021 acaagtttga cattgctagc ttggcttttg ggtggtttct taacgatctg tgctggattg
     1081 acagtggctg aattagctac agcaatcccg gaaactggtg gagctgtaaa atatattgaa
     1141 gctgcgtatg gaaaattgcc cagcttttta ttaggatggg cgcaaagctt gatttacttt
     1201 cctgctaata ttgctgcatt atcgattatc tttgcgacac aattgacgaa cttattacaa
     1261 ttatctaccg attatctttt gttgattgca gtgatcaccg ctgtttctgt gacagggttg
     1321 aatttgttag ggacaaaagt cggtgcttct gtacaatcag tgacgttgat tgttaaatta
     1381 ataccaattg ctgtgattgt catatgggga ttattgaccc caggacaagg aactgtccag
     1441 ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg aaggattgag cagtgcgttg
     1501 ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg
     1561 aaacggccgg aaaaagattt gcctaaagca attattcttg gtttgagttt tgtaacagtc
     1621 gtttatctat tgatcaattt tgtttttttg aaaactttgc cgattgatca tcttgccggt
     1681 aatttgaatg cagcttctga agcatcagac gtcatttttg gaggaatcgg tggaaagctg
     1741 gtcactattg ggatcttgat ctcggtatac ggtgcattga acggttatac actaactggg
     1801 atccgcgtgc cttatgcgat ggctttagaa gacgagctac ctttcagtaa acagctgaca
     1861 aagctttcaa agaagtttac ggttccttat gttcctgcgg ttttccagtt agcagtagct
     1921 tgtattatga tgagccttgg ttctttcgac tttttaacag acatgttgat ttttgtgatg
     1981 tggctattca gtttactgat ttttatcggt gtctttattt taagaaaaaa agcgccggaa
     2041 ttgccacgtc catataaagt accactttat ccaatcgttc cgattattgc gattctcggt
     2101 gctattttca ttttaggtat gacgatgatg acgcaaacga aattagcatt gatcggtata
     2161 ggtgttacct tgataggtat cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag
     2221 taaaatattt caacaacaga cagaaaaagt acgaactagg tcctctagat cttcgtgctt
     2281 tttctatttc aataaaacaa gttcttctat tttgattttt gctacttctg caagtagtgc
     2341 ttatgtgcta aagtaagaat gaaaaggaga gataaacatg atatttgatt ctcataccca
     2401 tttaaatgct gaacagttca atgaagatat tccagaaacg attcagcgag cacaagaaat
     2461 gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca acgattgaaa agtctttgca
     2521 attaagccat gactatccaa atatatacag tattatcggc tggcatccga cagaggctgg
     2581 cagttatacg aaagaaatcg aaaataaatt acaagaacaa ttgactttac caaaggttgt
     2641 agcattaggg gaaattggct tggattatca ttggatggaa gacccgaaag aagtccaaga
     2701 taaagtattt cgcagacaga ttgctatcgc aaaagaaatg aacctaccaa tcagtattca
     2761 tactagagaa gcacttgaag acacgtatca aatcctaaaa gaagaagatg ttcgagatat
     2821 cggtggaatc atgcatagtt tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt
     2881 agggatgcat atttcattta gcggtgtcgt cactttcaaa aaagcattag acgtccaaga
     2941 ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa acagatgccc cttatctggc
     3001 accagttcct tttagaggaa agcgaaatga accaggctat actcgttata cagtagaaaa
     3061 gattgctgaa cttcggaatc ttccgacaga agaagtggct gcccaaacat ggacaaatgc
     3121 gcaccgatta tttggtctaa gcgaacatgg ataagatgaa gatccaagag attatagtcg
     3181 ttgaaggaaa agacgataca agaaggctgc gggaagtagt tgatgcagat acgatagaaa
     3241 caatagggtc tgcaatcaat gaagagatcc tgatgcaaat tgaacatgcc caagagactc
     3301 gtggagtgat cgtttttacc gatcctgatt tttcaggaga aaaaataaga aaaatcatta
     3361 tggaagctgt tcctcaggca aaacatgctt ttttgccgcg cacacaagct aaaccaaaga
     3421 aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa
     3481 aggtcgtgac gccagacatt tcccaaacgg ctgtttctga aatcactaga caagaccttt
     3541 tagactatgg tttaatagct ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg
     3601 agttgcgcat cggttacacg aacggcaaac aattggaaaa acgtttgaag atgtttcgta
     3661 ttacgccgaa agaactcgcc caagcaatga aaatagtgga ggaacaacat gagtgaatat
     3721 cgtgaaattg cgacaccctc tagaacaaaa gagattttat tgaaacatgg tttttcattt
     3781 aaaaaaagtt taggacaaaa ttttctgact gaaccaaata ttttaaggaa aatcgtagaa
     3841 actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg gaccaggaat cggtgcattg
     3901 acggaacaat tagccaagca tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg
     3961 atccctgtat tggctgatac gatgcaaccc tatgacaatg tgactattgt acatcaagat
     4021 attttaaaga ccgatttgtc aacagctgtt agagaaacat ttcatgagga gcttccgtta
     4081 aaagtcgtag cgaatttacc ttactatatt acgacaccga tcatgatgca ttttcttgaa
     4141 tcagatttgg ttgtggatga actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc
     4201 tctgctgaac caggaacgaa agcttatggt tcattatcga ttgctgttca atattatatg
     4261 gaagccagtt tagcattcat cgtaccaaaa acagtatttg tccctcagcc aaatgtagat
     4321 tcagcgattt tgaaactgac aagaagagat ataccggcag tcgaagtgac agacgaaaaa
     4381 gcatttttcc gattgacaaa agcggcattt cagcaaagaa gaaaaacgct ttggaataac
     4441 ttgcagcatt cgtatggcaa agatgaccag acaaaagcat ggttagcaaa aagcttggaa
     4501 acagctggaa tcgatccaaa acgtcgtggg gagactttgt cattacaaga attcgccgca
     4561 ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga
     4621 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatgttttt
     4681 cgtttgtttg ggcttgttac cgcagatgca agcttttgaa caccgtttaa gaaagaggtt
     4741 gtgggcctgc cgtttaactc taagtcacaa ggaacaattt caaaaaacag cttctcatgt
     4801 ttttgagtta gttggtctta ttacttgatg caagcttttg accactatta tgccgaacaa
     4861 ggagatgtga cacaaatttt gtcacatctc ctatttttgt ttcttgatca aaaaattctt
     4921 atacttgata ctcacactca ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt
     4981 attagtaagt gagttatact aagtcgacat ttatgtgtca agacatatta ggagagagaa
     5041 aaaatgagaa aaaattcagt tcgaaaacta acgatttctg ctttattgat tgggatgggg
     5101 gtcattatcc caatggtcat gcctaaaatc atgattccac cagcttcctt tactttggcg
     5161 tctcatgtgc cattatttat tgcgatgttt tttacaccag gagttgcagt ggctgttgcg
     5221 ctaggtacga catttggttt ctttttaaca acgccggtta tcatcgcatt gcgagcattg
     5281 tcacatcttg tgtttgcgtt gattggggct tggtacttgc aaaagcatcc gagtatcgtt
     5341 ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc
     5401 ggtgtgattc attctttagc agaaatgcta gtagtcagca tcttttactt tattggtaat
     5461 atgcctgaaa catattattc tcaaggatat ttttatacag tattcatctt gatggggatt
     5521 ggtgggctta tccacagttt agttgattat aatattgctt attttatagc aggaacactc
     5581 agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg
     5641 attcaagggt acgcgaagaa aacgacatat tagacatgat ctctcaaaaa aaaaactctc
     5701 atctagtaat ggataataaa tcctctaaga tgagagtttt ttgtcctaaa aagagctgca
     5761 ccaacatctt ctggcacagc tctaacaggg ggcaatttat ggaagtttga tgacttgttc
     5821 tgtttcttta tcaaagaagt ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc
     5881 tggtttatgg aaatctcttg catctacttt tgagatgaac tcagtagaac cagtttttgt
     5941 gtaaagcatc gtttcagctc caagtaattc ggaaacgaca acttctgatt tcactacggc
     6001 ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg tcttctggac gaataccgaa
     6061 gataacttct tttccttcat aacctttttc aactaatact ttgttttttc cttcaggaat
     6121 acgaagctta agcccatgtc catctgtgat aacaccattt ttcaatgtca cattaaagaa
     6181 gttcatggca ggagatccaa taaagccggc aacgaacata ttgacgggtg tatcataaac
     6241 ttctttcggt gtaccgattt gttggataaa tccgtctttc atgataacga ttcggtcagc
     6301 catagtcatt gcttctgtct gatcgtgagt aacatagatg gtcgttgttt caagacgctg
     6361 gtgaagtttg gcaatctcag cacgcatggc tacacgtaat ttagcatcta aatttgataa
     6421 tggctcatcc attaagaaga cttttgcatc ccgaacgatt gcccgtccca atgccacacg
     6481 ttggcgttgt ccaccagata aagcagctgg ttttcgtttc aagtattctg tcagacctaa
     6541 gatctctgct gcattgtcca cacgtttttt gatgtctgct ttatcatatt tacgtaattt
     6601 caagccaaaa gccatattat caaaaacagt catatgagga tacaatgcgt agttttggaa
     6661 aaccatggca atatcacggt ctttaggtgc cacgtcattc atgacttttc cgccgatatt
     6721 tagttctcct tcagagatat cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc
     6781 acaaccggaa ggaccaacaa aaacgataaa ttcacggtct ttgattgata gattaaaatc
     6841 agttacagaa taattttctg cattgtcata ttttttatat acgtttttca gagccatttc
     6901 taccataata cgttccacac tttctttttt agttttatta actatttaaa gtataaatga
     6961 aagcgttaac taacttcaat gtcatgttga acaagaattt tttctttttt ttgtcaatgt
     7021 gctagtttta agctttaata aatatccata agaaataaaa actgatacac tggagtaaat
     7081 aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa atcgctttga tcgctcatga
     7141 ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat aaaacaatat tggaaaaaca
     7201 tgaacttttt gctaccggga cgactgggca gaggatcacg gaagcaaccg gcttgccgat
     7261 ccatcggttt aaatccggac cgttaggcgg ggatcagcaa attggagcca tgatttcgga
     7321 ggatcagtta gatttggtga tttttcttcg agacccgctg gctgcacagc cccatgaacc
     7381 agatgtaaca gcacttatcc gtttatctga tgtctatgag attccgttag ctacgaatat
     7441 cggaacagct gaggtgctac taagaggact ggaagccggc ttcctagatt ttcgaagaat
     7501 cgtccatgaa atagacaagc atccactttc attttgatgg ataagcgtat ggaaaaataa
     7561 cgtttaatgt tcgcctatat gtagctataa aagctaaaca aactgataat atgcgatata
     7621 atacgaacgt attggataat ataaacaata atattagttt ttcgtatttg cagtaaatgt
     7681 gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa taatacagag tagaaaataa
     7741 agcgttaagt gctaagagga tgggatgttg cctcttggac gaaggacgta atgttcgcgg
     7801 ttttattttc gcattcgctg catgatacga tgtagcagct cttaaaggag atgctaaaaa
     7861 tgaagaaaca ccggttagac gcacgtatga ttgctattat gggcttattg attgcattga
     7921 tggtcacttt gtcccgactg gtagcaatcg agaccccatt tattaaaatc agtgtcacat
     7981 ttattccaca agtgatcatg ggcattcttt ttggtccttt ttggtcaggg ataggcgcag
     8041 ttttagctga tttagtagga atggccctct tctcgaaatc tgcattcttt attggattta
     8101 cattgaatgc atttattgaa ggagcaatct acggattctt tttctatcga aaagagatca
     8161 cttggaaaaa tgccattctg gctacgctta gtgtcacgct gatcatcaac ttatttttga
     8221 caccattatg gcttgcgttg atgtaccatg tgccattgtt cagctgggtc gtttgggcgc
     8281 cccgcctgtt aaaaacagta atatggttac cgattcaatc aattgccatt tattacgtag
     8341 gacgtagtat tccttacaaa aaaattttaa gaagtttagc gatccacgct aaataaaaaa
     8401 caagcagtct tcccatccca gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga
     8461 tagagccgtc ggtaataacc atctttttga agcagctcat catgggtgcc catttcgaca
     8521 atagctccct gatccatcac taatatctgg tccgcatttt tgattgtgga caggcgatgg
     8581 gcaatcacaa aactggtttt tccttccatc attgctaaga aggcttgttg gatttttttc
     8641 tctgtcaatg tatcgactga gctggtagct tcatctaaga tcaacatagg gggattactg
     8701 atcatcgttc gagcaatcgt caacagttgg cgttgtccat ctgatatctt taacccttga
     8761 ccgccaatct tagtatctaa tttttccggc aaacgttcta caaattcata catataagag
     8821 gcttttaggg catcattgat ttctgcgtca cttgcttccg gattcccaaa acgaagattt
     8881 tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca ccattccaaa acttttacgt
     8941 aatgtatcac gttggataga gcggatgtca tggtcatcga ctgtaattgt tccttttgtt
     9001 gtgtcataaa aacgcatcag caaattaaca agtgtggatt ttccggcccc tgtttttcct
     9061 actatagcaa tggtttgacc tggataagca gtaaaattga aatcttcgat caaacgctgc
     9121 ttggggtcat aagaaaaaga aacatgatca aaagagactt ttcctttgat atttttgggt
     9181 tccaaggcat aggcatctgg caaatcaggc ttttcttctg gctgatcgat caaatcaaat
     9241 gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg tgattccaga aagttcgata
     9301 aatggtttag aaaactgact tgagtagatc gtaaagctgg aaatcacacc aacagtgatt
     9361 gacgaaccac tcttcaatgc caataggccg ccaactaaac caatcgataa ataagcaagg
     9421 tgatctacaa aacgagaaga cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct
     9481 ttttcatata gatcagcatt tagcagttca aattgctgtt gactagcttc ttcttgctga
     9541 aaagctttca cgattttctg gttaccgatt cgttcggtca caaagcccga gatatcccca
     9601 ataatccttt gctgtctatc gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca
     9661 aggaaaataa ttggtgtaga acaaagaacg accaatgtca gaagtggact tagacgcatc
     9721 ataaaaataa acgaaagaat gatgattgcc actccagaaa ataattgatt gaaggccgca
     9781 gaaattgcaa tggaaatatt gtccatatca tttgtaaatc gactgacgat atttccgtgg
     9841 gagttttgat cataataacg aagtggcaat cgattcagat gagcaaatgc atcttttctt
     9901 aattttgcaa cggataagta agctaaccga ttgcttaaca cttggatgag ccattgactg
     9961 actacgttga ttaaaacgat accaccgaat agaataagta aatggaacag tcgattgaag
    10021 cggacctgtc cttctccaat catttgatcg atactttgac caatttgtaa cgtcatcaca
    10081 actgttgcaa tcccattgat gattcctagt atcacagcta gccaaatgtc ttttgcatag
    10141 gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg ataatttttt tactttcata
    10201 actattcttc ctcctgtgat gcgacgattt cctgatattc tgctgattga gcaagtaatt
    10261 cttcatgggt accctgagca gcaatcttac cttgatccat cactaagata tgatgtgctt
    10321 cttgaataga gcggacacgt tgggagataa taatcactgt actgccaaga taagtctgta
    10381 gcgccttacg caaatccaag tctgtttgat aatccaacgc acttaaagag tcatctaaaa
    10441 tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc
    10501 cagagaaatt tttcccattt tccatgactt ttgtatctaa accatttgga agctgacgga
    10561 caaattcttc tgcctgagcg attttcagag cttcccaaca ttctttttct gtggcgtctt
    10621 tcttacccca ttgaaggttt tcacgaatag tcccggtaaa tagtacagaa gtttggggaa
    10681 caagagcaat ttgctgacgg agatgatcca gctgccaaga acgaacatcc attccgtcca
    10741 ttataactgt accggaactg acatcataga aacgcggaat caattgtgtc aaagtcgttt
    10801 tcccgcttcc ggttggacca atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa
    10861 tattttggag agaaagacca gaatcaggag tataacggaa gtcaacatgg tcaaaagcga
    10921 taattgcttc agaagaaaaa tcgggagtag tactgacctc agcttcaaga atcgaatttt
    10981 ctgtgtctag cacctctttc acccgatttc ctgaagcttc ggcacgtgtg aagatcacga
    11041 cgaggtttga aacaacgatc agtgccaaaa gcatttgatt catataattg atcaaagcaa
    11101 gaacttgtcc ttgttgcaag ctaccaatat ttactttgat cccgccaact gtcagcaaac
    11161 agatgatccc gacattcatg atcaaggttg tagcaggtga taatagggca gagatattgg
    11221 aaacacgtaa ataattgttc gctagatcat ttgtgctttt atcgaacttc tctgtttctt
    11281 tttttgtctg cgcaaatgcg cggatgacac gaacaccgct aagattctga ctaacgtgtt
    11341 cattgagctg atctagtttt tcttgcactt tttggtagag aggaacggtt tttttgatga
    11401 taaaaaataa aatgattgag aaaataggca ataatgctaa gaaaaacaga cctacttccc
    11461 agtcgatcac aaaagccatg accactgacc cgatacttaa aaacggtgct ctgatcaata
    11521 aacgaatgac cattgctaaa gctagctgaa gctgattgat gtcgtttgtc attcgggtga
    11581 tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga gaaagtattg atttttttga
    11641 ttagctgatt acgtaactct gtaccaaatc cttgtgaagc tactgaagca tagtattggc
    11701 aaatgatagc acaaatcaga ccaatcacag acatagcgac catccataag cccatttcaa
    11761 tgatttttcc tcggtcattc atttttagtc cattatcgat caaggaagcc atcattaaag
    11821 gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa cgggcccaaa atgatttgct
    11881 tacgatagtc ttttgcataa cgtaataact gaatcatatg attcctccta ttatttattt
    11941 gtccacaatt gaaaaaacaa gtactaccac attgtagctt aggaaattta gaatgaaaag
    12001 agaacaagcc ttttcataaa tgaaagtttt attatgaaga agctttagaa at
//
LOCUS       DARBER010000080        11989 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARBER010000080 DARBER010000000
VERSION     DARBER010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.081"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SCK06_001934"
     CDS             complement(221..1423)
                     /locus_tag="SCK06_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1151930.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SCK06_001935"
     CDS             complement(1426..1620)
                     /locus_tag="SCK06_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1151931.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SCK06_001936"
     CDS             complement(1667..2515)
                     /locus_tag="SCK06_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG1151932.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SCK06_001937"
     CDS             complement(2720..3070)
                     /locus_tag="SCK06_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151933.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SCK06_001938"
     CDS             3665..4111
                     /locus_tag="SCK06_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1151934.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="SCK06_001939"
     CDS             4735..5727
                     /locus_tag="SCK06_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1151935.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="SCK06_001940"
     CDS             5743..6627
                     /locus_tag="SCK06_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1151936.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="SCK06_001941"
     CDS             6676..8283
                     /locus_tag="SCK06_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1151937.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="SCK06_001942"
     CDS             8284..9768
                     /locus_tag="SCK06_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG1151938.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="SCK06_001943"
     CDS             9945..10937
                     /locus_tag="SCK06_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1151939.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="SCK06_001944"
     CDS             10989..11858
                     /locus_tag="SCK06_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1151940.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3714 a         1960 c         2484 g         3831 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatat
//
LOCUS       DARBER010000081        11973 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARBER010000081 DARBER010000000
VERSION     DARBER010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.082"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..324)
                     /locus_tag="SCK06_001945"
     CDS             complement(<1..324)
                     /locus_tag="SCK06_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1151941.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPET"
     gene            complement(440..1354)
                     /locus_tag="SCK06_001946"
     CDS             complement(440..1354)
                     /locus_tag="SCK06_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1151942.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(1445..1873)
                     /locus_tag="SCK06_001947"
     CDS             complement(1445..1873)
                     /locus_tag="SCK06_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1151943.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(1878..2252)
                     /locus_tag="SCK06_001948"
     CDS             complement(1878..2252)
                     /locus_tag="SCK06_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1151944.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            2538..2723
                     /locus_tag="SCK06_001949"
     CDS             2538..2723
                     /locus_tag="SCK06_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1151945.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            2735..2983
                     /locus_tag="SCK06_001950"
     CDS             2735..2983
                     /locus_tag="SCK06_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151946.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3014..3193
                     /locus_tag="SCK06_001951"
     CDS             3014..3193
                     /locus_tag="SCK06_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151947.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3236..3706)
                     /locus_tag="SCK06_001952"
     CDS             complement(3236..3706)
                     /locus_tag="SCK06_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151948.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3793..4491
                     /locus_tag="SCK06_001953"
     CDS             3793..4491
                     /locus_tag="SCK06_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1151949.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4669..5010
                     /locus_tag="SCK06_001954"
     CDS             4669..5010
                     /locus_tag="SCK06_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151950.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5003..5674
                     /locus_tag="SCK06_001955"
     CDS             5003..5674
                     /locus_tag="SCK06_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1151951.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5680..6366
                     /locus_tag="SCK06_001956"
     CDS             5680..6366
                     /locus_tag="SCK06_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151952.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLSH
                     ETIKKLRIGG"
     gene            6369..7199
                     /locus_tag="SCK06_001957"
     CDS             6369..7199
                     /locus_tag="SCK06_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG1151953.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            7215..8066
                     /locus_tag="SCK06_001958"
     CDS             7215..8066
                     /locus_tag="SCK06_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1151954.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            8063..8422
                     /locus_tag="SCK06_001959"
     CDS             8063..8422
                     /locus_tag="SCK06_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG1151955.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            8437..8598
                     /locus_tag="SCK06_001960"
                     /pseudo
     CDS             8437..8598
                     /locus_tag="SCK06_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            8595..8900
                     /locus_tag="SCK06_001961"
     CDS             8595..8900
                     /locus_tag="SCK06_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151956.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            8900..9211
                     /locus_tag="SCK06_001962"
     CDS             8900..9211
                     /locus_tag="SCK06_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG1151957.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            9208..9381
                     /locus_tag="SCK06_001963"
     CDS             9208..9381
                     /locus_tag="SCK06_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151958.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            9378..9794
                     /locus_tag="SCK06_001964"
     CDS             9378..9794
                     /locus_tag="SCK06_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1151959.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            9794..10462
                     /locus_tag="SCK06_001965"
     CDS             9794..10462
                     /locus_tag="SCK06_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1151960.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            10459..10836
                     /locus_tag="SCK06_001966"
     CDS             10459..10836
                     /locus_tag="SCK06_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151961.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            10833..11132
                     /locus_tag="SCK06_001967"
     CDS             10833..11132
                     /locus_tag="SCK06_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151962.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            11122..11280
                     /locus_tag="SCK06_001968"
     CDS             11122..11280
                     /locus_tag="SCK06_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151963.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            11374..11790
                     /locus_tag="SCK06_001969"
     CDS             11374..11790
                     /locus_tag="SCK06_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1151964.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         4224 a         1842 c         2490 g         3417 t
ORIGIN      
        1 agtctcaggt atctttttct ttatagtgtt taagatatta ttcgtaggat aatatgtaga
       61 ctccttaact tgtcttttgt aaataactaa ccattcttcg acaagctcat gaaatgttag
      121 atcaggcttc tgaagtgttt tttcgtttaa ttttatatca atttttttat ttaactctag
      181 caatgccact ttctgagttt ctcgagattt atttttataa gtaatgctca ctttttttct
      241 ttttctagtt tttggatcaa tatatcgctc attatattta tatacttttt ttccgttttt
      301 atcagttttg gtttcaatcc acattgtttt catctcctat ttgttgctat aataggaatg
      361 gataagtaag ccaattatag caggtttatt tttcatcacg tccacaaact ttggcgaggg
      421 agggggcgtg tttttttgtt tagtagctac ctttccatga agtatctgcg aagaacactt
      481 caaatgttgt tccatcattt ttcacttcaa aatatgctgt tccttgtgcg gatttacccg
      541 gttggatggt ttccgaaaag taatctctac tagctaaatc agatttaata tctgatgaat
      601 cataaaattc taaataactt gaacttgcat caaaaggtgt agtccctgtg ttttctacaa
      661 gaaaatctac ctttgcgtaa aataggcctt ctggtttata gaaatcatca ccgtaatctt
      721 ttgatacaga ctggacagtt acgttaatag agttaccatt ttcagaagta aaaggaactg
      781 cttctcctat actatgtgca actgatgccg tctggggttc tgaagacgaa gttgtagttg
      841 tagttgtagt agagcttgaa tctttggatt ttccatcttt atctaagaaa gttcgttgat
      901 tataatatac aacctgttca gtttttggac taacccaaat taacattgtt tcatcatcag
      961 aatcagttga ataagtatat tggagcatct ctaatttttt tatatctttt gcactatttc
     1021 catgttcaat taattcagag tgctctttat aataatctcc ttgtgcatcc tttgaaatag
     1081 atacaattaa ttcatcccgt gtgatagcat tgtctaattc tttgtaaaca aaatcatttg
     1141 tccatttacg taacggtgaa ccaagtgtct gataaacttg agttaaatca tcaccaactt
     1201 ttattttatg aacgtctgat gaagttagtt tttttgtttc atattcagat gttgatgaaa
     1261 cagtattttc tttagtgcta gcagaattag gactagtgga gcatccgctt aataccaagc
     1321 ctaaaaaaag caacccaaaa acaatttttt tcatttttaa ttcctcactt ctatgatatg
     1381 atttttttgt aggatcttag aaataaggtt tttagtccgt gttgcagcac ggactttttt
     1441 tacattatat aagaaagcga taagcgcttt ctggaagtcc gtaaagattc tttaattcct
     1501 caattcgttt aggatattga tcattgtctt ctttgtatag agaaacaatg agattagcag
     1561 caaagcaatt agcttcgctt tcagatttgc ttctagatgt tcttgttgat acatagtaac
     1621 tggataagcc acgatgaaaa atagcgtgac ctaattcgtg agcgcaaatg tagaatcttt
     1681 cctcagagtc tctcagttca tcatttaaga agattattgc acgacctcta atttcttgaa
     1741 actgtccttt gggattctcg ataaaaggaa cgtattgaat tttaatgccc atcttttcac
     1801 aaatataaaa aggattagcg gactggtatt tccgcttcaa ctcctcgact aaattaatcg
     1861 tatccatctc cataagctca catctttttg cctttttctt tgtcttcttt cacaatatcc
     1921 cagaaggttc caataaggat atcttttacg cgttggacct gttcaggcgt cagtgtttct
     1981 ccgccataag acatattgac atttgaatct agtaatttat caagttcaac tacttcatct
     2041 tttgttgccc agtcaggaac ttggtttcta ccgagtaaat aatcagtagt tacattaaaa
     2101 taatctgcta caagttgtag tcttttagta ctaggagttc tatttttcca ttgataaatc
     2161 gtgtttttag gtatatttaa ttcttcttcc aattgagtta tacttatgcc tcttttatga
     2221 gctaattcct ttattctatc tagtaaattc attttcgcac ctcacaagct acgaaaacac
     2281 taataaaaaa attagttaaa ggtgttgaca actaataaaa gtgttagtat actgttttcg
     2341 taagctaaaa tattagctaa aaagacttga taactaataa acacttcacg gtcggcaaac
     2401 tctgaaatgt aaattactag gcatttctgt gtcttattta gctatgtcta tatactaata
     2461 aaaatattag ttattgtcaa cgaatattag ctaatttttt agcttacaga ttattttttt
     2521 agaaaggagc tatttttatg tctgagaatt tagacttaaa aattcgagcg gagatgagaa
     2581 aaagaagaat gactttcaaa gaactagctg cgcttatcgg tatttcagga gcttatttat
     2641 cagatattct aaacggcaat cgtgatggaa agaaagcaca acagcatatc gaaacagtga
     2701 aaaaaatatt ggacatccga taggggggta ggcgatgaca aaactaaaaa aacaagattt
     2761 tgtaaaaaaa tacaattatt ctccatctac ttatcaacgt cgaatgtcgg aactaaaaaa
     2821 tacagcaatt ttctcagcgg cgtatgaacg ggtcacagga caagaagttt ggatcaatac
     2881 agaattatac gataaatttt tgtctttcaa atcctataac aggttacgca caagaaaggt
     2941 aacacctaaa gaatttatcg agaagcattt agttgattta taaaaaacag aacattttga
     3001 gaggtgaagg ttaatgggta aattcaacag agcattggta ttcagcgcac cgctaatcat
     3061 ctacgcttta ggactttggg gaagcaggca agcgttgata ggaacgattg tttacatggt
     3121 ttggattttt atggggcttg atgaagctga agctgagtac agagcgaaaa agccaaccga
     3181 gggggctgac taaaaatgaa tagaaaagag aaactagaat gtatattact attactcagt
     3241 ttaattcttt cactaatttc tctattggga agtttgtatt tttgatatca acaattttgt
     3301 ggatttgggt tctatttaga gtaaatatca tttttgtttg gtgattaagg agtttattaa
     3361 aattaatttt ccctacttca aatgcaaggt aacaagatat agcactttta ggaggaatat
     3421 ttagcggtac agcttcagag tatattctat tatcatcgac tgatcctcct ttagcaatta
     3481 atttcttaaa tggagtagct gaccatctgc ggtttaagtt agcggatgaa agttctaaat
     3541 taactaacat agctggttct gttgaatagt tagaaatgat tacttttgta taaatcatat
     3601 ctgcagcgaa gtaagaagca ttaagttcaa cttctagttg agggcgcatt ccttttattt
     3661 ttatccctgt taaaaacgta ctcaatataa aacttattat tgacatccat tgaaaaattg
     3721 ttaagtgaaa aaaattcaaa ataaacacca ccagttttta actaaattat accaaaaagg
     3781 agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc
     3841 ttacaagtgg cagaaagctt cgagaaaaaa catcaacatg ttttaagaga tattgatgca
     3901 ctaaaaaaag atgtgtccaa ttttggacag atgtttgtag aaggtaatga accagattca
     3961 tatggcagaa atcgacgagt tttcttcatt agtagagatg gatttttctt gctggctatg
     4021 gggtttacag gaaagaaagc tatctacttc aaacaaaaat acattgaagc attcaacgaa
     4081 atggaagatg ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat gatgatctat
     4141 caattagaag aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga
     4201 attagcggac aacaagagtt tgaaattaag caaaaaggaa atatgaaagt tatgggagtt
     4261 ctagggggaa aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt
     4321 tggtctgaat ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga
     4381 ttcgatgatg ctgtttcatt tattgaaatg tggttgccag aaactgcgat ccgtatggaa
     4441 attgatcaac tgaacagaca acagagactt tttggtgatg acaatgaata gagctgaagc
     4501 gctaagaata gggacggcaa ttgctaattg ctggtggaaa tactataaac caatcatcct
     4561 aagccaacaa catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg
     4621 gcaagcaaag agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa
     4681 ctttccactc tagatcaata tttgattgat cctgattggg gcaagccgaa aattgaggaa
     4741 acaagtggtc gaaaaatcag acgaaatctt ttgacgaatg aagaactagc ttgggatcaa
     4801 gatgatttag gcaaccatgt aactatttgg gatcatgttt atcttatcca tctatcgaag
     4861 cattcgaata aacctgaata tatttacgtc atcgaagatg gcttgattga tgcgctagaa
     4921 gagtacgaca gagataactt gattgatatc tcttattacg gaccaggtaa gaaatacatt
     4981 gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact
     5041 ttttagtcgt aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg
     5101 ggctcacgcg tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga
     5161 gtacaaacac tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa
     5221 accttttctt atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca
     5281 cacggaaacc gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa
     5341 tgcaatgcaa atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg
     5401 actgggatta tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacaaa
     5461 gaaattaaac atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat
     5521 gaccaaaacc ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga
     5581 taacgttgaa actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat
     5641 agcagctaag agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat
     5701 tcatattcag cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac catcaatgag
     5761 gaagtgaaca ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagtcatt
     5821 gaaattcgtt ttattgatcc tagacgtttc accgtacagc aacgaaactt catctatgcg
     5881 ctgataggcg atatttttat cgatacaggc atgccaacgg acttctggaa ggaattcttc
     5941 tacttccgtt ttgaaggtgt cacagggcgc aaaataagcc tcaaagatga atcgaatacg
     6001 actgtgagtg atgctaatgt cttagcaaat atcatcttag atttcatctt tgaacatcat
     6061 attcctttca aagaaggcta tgagatttta ccagcgaatc aagaatatta cttctacaaa
     6121 tgcattacaa aaagagtttg ttgcatctgt ggcaaaacag gagctgacat cgatcacttt
     6181 gacaaagcgc taggaagacg aaagcgaaaa gaagttgatc attcagagta cacatttgca
     6241 gcactctgca gaatccatca cacggagaag cacaaaatag gtgtgatcaa ttttaaaaat
     6301 aaatatcaaa tcaaaggaat caagttaagt catgaaacga ttaaaaagtt aaggatagga
     6361 ggataaattt ggctgagata agttggatca aacttaaaac tactatgttt gacgatgaaa
     6421 aaatacggtt aatccaagct gttcctgagt cggatgccat catcgttata tggattcgat
     6481 tactagtttt agcaggaaag actaacgacg atggtctgat atatatccag aggaacatgc
     6541 cttataccga agaaatgctt gctacattgt ttggcaaaaa cgtaaatacg gttcgcttag
     6601 cgctaactac attggcaaat ttcaacatga ttgatctaag cagtgatgga ctaattgcca
     6661 tcagtaattg ggaaaaacat caaaatatcg agggtatgga taaagtaagg ctaaaaaatg
     6721 ctgaaagaaa ccgtaaatac agggaaagaa agagacagga acgtctcaaa ttggaaaatg
     6781 acgttagcgt gacgtcacgt gacggtacag ataaagatat agaagaagat aaagatatag
     6841 ataaagaaga aaagaaaggt aagtattctg acgaacactt acgccttgct aaaaaaatgc
     6901 aaaggaattt aactgaagat tttccaaaag aaatgaacaa agtagatatc gaaaaatggg
     6961 cagacacaat caggttgatg gaagaaagag ataaagcatc tatagaagcg attgagtatg
     7021 tgatcaattg gctacctaca aatgaatttt ggtttggaaa tattagaagt gctaagaaat
     7081 tgagagaaaa atttgagaag ctcaaattcg aaatcaaagc agacaagaag aatcataaaa
     7141 agcaaagtca aaaactacag tacagcaatc ctagtgaata tgacgacttg ccaatttaaa
     7201 aggagatgca tcacatggaa agcctagcaa atgctatgga gaagctaata agaagagtat
     7261 tagtgcaaag cggaaaatgt ccagaatgta gcgaaccttt gtatagttgg cgagctaaaa
     7321 ataaggatgg ttcagaacgt tgtaaaccaa catgtatgag ttgtggttat aaagcgttac
     7381 gtgtgaaaga ggatatacag accgaacgga tatataacga cagcttaaaa gcacgagcat
     7441 tgagtttttt tcaaaatggt tcggtattaa cagataaaac tttgtttaaa tgcaaaatgg
     7501 agaattatca cgtagtggac caagaaacga aaattgcttt agaaagagct aaaagctatg
     7561 taaatgatgt cctactgaac catcctgcac atttcattct atcagggaaa tcaggaagcg
     7621 gaaaaagcca cttatcaatg gcgacagctt gggaaatact tgagcgctca aattatgaca
     7681 agaaaatact ttttataagc tatcaagagt tattagagca aataaagttt tcttataaca
     7741 atgctgaact gagaaaagaa attgaaggat cgcttatagc cgatatcaaa acaactgatt
     7801 tggtggtttt tgacgatatt ggagctgaat taggtagcgg ggtatcaaat agtaggcaat
     7861 ttacaaacaa cacgttaaac acgctcttag aagccagaca gaacaaggca acgatcatca
     7921 caacaaactt atctggtcct gaactaagag aagcctacgg tgaaagaatt gtttctagga
     7981 tatttaagaa ttcagaaggt tatgcgctga aattccaaca aacagcagac aagcgcataa
     8041 aaccagtgaa aggtagtatc gcatgaataa ataccgtaat aaaaaaactg ttcatcgagg
     8101 tatcaagttt gattctatcg cagaagcaga gtattacgat ctagccttgt ggcaagctga
     8161 agcgaacggc tggaaagtaa aacttcagga aagatttgag ctgatgccga aatttgaact
     8221 agacggaaag aagtatcgca agatcgagta tattcccgac ttcacatttt ataaaaacgg
     8281 caaactagtc aaagtcgtag atgttaaagg gatgcagaca aaagatttca aaatcaaagc
     8341 gaaactcttc tgcagtcaat atcgagtacc actgatctta gcgaagaaat atcggaatac
     8401 gttcaaggaa gaacggtttt aacgaggtga tccatcatga caacagaaga agtgattcaa
     8461 atgcgtattc gaagccttca gcgtgagatt gacgatctgt aacggacaaa ggcagtgatg
     8521 gtcaatgaaa cggcgaggaa ggcaatcgat ttgcacatag aaaatttaag aaggaaaatc
     8581 catcgattgg aggaatgagc gtggataaga aagcagcaat gaaacgaatc atcgaactga
     8641 cacattctga gaattggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta
     8701 aatcaatgtg ggctgaaaag cctaaacgga aaacgccgag aaaaattgca atctggcatg
     8761 gtgatcgaat tctagtaaca ggtactgctg aacagttatc tgaaattact ggattaagca
     8821 aaaacattat ctgggataga gctaggagct tatggattga ttcaaaagga cgacagttta
     8881 ggtatgtgga ggagaaataa tgaacgaact aatcacaaaa gtagagcagt gggctaaaga
     8941 caagggactg gatcaagcgg atccaaaagc acaatttttg aaagtagctg aggaattcgg
     9001 agaaattgca tcggcaatgg caagaagtaa tgatgagcta tttaaagata gcgtaggaga
     9061 cgttatcgtc acgctgatta ttctttccat gcaaaaaggg gcaaacatac aagagtgttt
     9121 agaaatggcg tataacgaaa tcaaaggacg cacaggaaaa atggtagatg gtgtattcgt
     9181 gaagtcgagt gatttggagg acagcgaatg atgatgccaa aagaaatcta ttcagatgca
     9241 ttaagaggtg ctagaaatca attgaaaatg gcaaaaagag catacgaaat tcggcccaca
     9301 atcgaaaatg aacgcagagt aaaagcaatt cgccgtagat gttcaattta cggcgagtta
     9361 cagaaggagg acagcaaatg ataccgagat ttcgagcttg ggataaacga aagaacgtaa
     9421 tgagagatgt agccgtcttg cattttacta aaaacggcaa aacaaacttt attgaatatt
     9481 ggataaatcc taccgaattg aaatcatatc acgtgcgaaa catcgagctc atgcaatcaa
     9541 cagggctgaa agataagaat ggcattgaga ttttcgagtg ggatattgta tcagtcagcg
     9601 tgcgaaatgg attcgattat ttagataata aagtttgtgt tgtcaaaaat tcaataggac
     9661 attcgggatt agtgtgtgcc actgttgatg aagatttaga gtatcaaatt tttaacacag
     9721 agctgtttga agaatacacg tatgaagtca tcggaaatat ttgggagaat agcgagttat
     9781 tggaggaaca gtgatgaata aacaggaatt gattgatgaa ttagctaaat atgtaaagcg
     9841 ttatgagaac gttatggatg agcatggcca aggaaggtac ggcgcttatg aagtatcttt
     9901 aaagttggtg aaaagactaa atgaatcaaa aattacggac gaacaagctt ggaataaggt
     9961 agctgaggct tatcctgaat cggcacaaag cttgagaaac actttagata atgctgtatt
    10021 tggtaagact ggtgaaccgc agaagcacgt tatgccgaag tttgtggcgg agtggataga
    10081 atacgctaaa aagaaaggtg atagtctagc tatttcattc aagccgtgga acctctatgg
    10141 tgttgagtat agcaaggctg atagatggat tggagataat caagaaacgt ttgctcgtgc
    10201 ttggctagac ggctacgagg tcgagaaaga gccgttgtat tatgtcccgc taccatatga
    10261 agtttgggat gaagaggcag cggagcttaa aacagagtat ttgtatctac attatgagat
    10321 cacaagtgat gaaactagaa tatttcctac aaaagaacct agaaaaggtt ttgtcgcaaa
    10381 attagatgaa cttacgatta aatcagcaga tgaaaactac tggccgtttg ctgtaccagt
    10441 ggaagaggtg gtagaaggat gaaaaaattg caccaactca tatctgagaa agaaagcgaa
    10501 ctccaaaatt tagaagatag tttgggattg gggtttccaa ttgttgagca agttaaaatg
    10561 gttcaaatca gtcatctaca acttgaattg gaagatttaa gacaaataga agatccgtat
    10621 caactcaacg ataaccagca aatcgtgttg gagtggttga aactaactgc gtcaacagga
    10681 aagccaatgc aagttgtatt ttggatgatg aataatgcag catgggggca tttagacgaa
    10741 ttgagggatc ctttgatgga attaactgac aaacagcaat tcgaagtcct aacagcattc
    10801 gctcaatggg gattagaaca ggaggaaaaa gaatgaaact aaaagacgga ttttacgcta
    10861 gtagccacgg tatcggcggt ttaatgctag acatgccgac aaagaaccct aaaacacgta
    10921 agaagccaaa agtcaaagtc ggtgacatgg ttcgctgtga agcagaagga ttcatctatc
    10981 catttcgtgg atatgtagaa cacgtctata atcactcagc gatcattcgc attgaaaaca
    11041 cgatggaatg cgacaagtta ttagcgaaaa gcaaagataa tttagcagtg gtgagattgg
    11101 tggatatgga ggttataaac aatgaaattt aaaatctttg aagaggacac tcgctataaa
    11161 ttagaaaaaa aattaaatga atttgcaaag aataatgaaa ttcagcatat atctttaacg
    11221 gcttctaagt caggttatac aacttactat gcagctgttg tgagctacgt aattcaataa
    11281 aaggaactcg gcaaataaaa aagccggatc actccgactg atgtaataaa tccgacaagt
    11341 ttattatatc acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc
    11401 aaacagacaa gatgtaatgc gagagatgtg ctgaagaact ttcggcgctt ggagcggatg
    11461 gcaggtcgct ctttgataga tattaagtcg ccgattatta cggatatgcc gaaggcaccg
    11521 aagcacggca ataaggcaga agacgcgatc attcagatga tggatataga agcggagaga
    11581 gacgcgattt tagcggcttt gatggcgctt agtctgatta gccgtcagat actctactac
    11641 agcttctgtg acgtaaacaa gcactctaat tatgaaatag ggcaattgat acgaggatac
    11701 ggagagaaga atgtagagaa gctgaaatcc atcgcattga tcgaatttgc tgaagcatac
    11761 aaaaaaggcg tgttagttca gtatcgttaa ttttgtaggg tttttgtagg gatagtgtag
    11821 ggtttttgag cggtttaacg tgatattatg atagtgtcga aagattagtg ataggtctaa
    11881 gacaaaataa taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag
    11941 acagcagcgg aaatattaag aataaggatg tga
//
LOCUS       DARBER010000082        11526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DARBER010000082 DARBER010000000
VERSION     DARBER010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.083"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            139..987
                     /locus_tag="SCK06_001970"
     CDS             139..987
                     /locus_tag="SCK06_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG1151965.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1132..2073
                     /locus_tag="SCK06_001971"
     CDS             1132..2073
                     /locus_tag="SCK06_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG1151966.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2063..4234
                     /locus_tag="SCK06_001972"
                     /pseudo
     CDS             2063..4234
                     /locus_tag="SCK06_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(4346..6517)
                     /gene="copB"
                     /locus_tag="SCK06_001973"
     CDS             complement(4346..6517)
                     /gene="copB"
                     /locus_tag="SCK06_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG1151967.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(6537..8723)
                     /locus_tag="SCK06_001974"
     CDS             complement(6537..8723)
                     /locus_tag="SCK06_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG1151968.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(8723..8932)
                     /gene="copZ"
                     /locus_tag="SCK06_001975"
     CDS             complement(8723..8932)
                     /gene="copZ"
                     /locus_tag="SCK06_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG1151969.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(8945..9385)
                     /locus_tag="SCK06_001976"
     CDS             complement(8945..9385)
                     /locus_tag="SCK06_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG1151970.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9459..9998)
                     /locus_tag="SCK06_001977"
     CDS             complement(9459..9998)
                     /locus_tag="SCK06_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1151971.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            10246..>11526
                     /locus_tag="SCK06_001978"
     CDS             10246..>11526
                     /locus_tag="SCK06_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEG1151972.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT         3323 a         2412 c         2089 g         3702 t
ORIGIN      
        1 acaatgaaat aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa
       61 gaatccaaac aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa
      121 cgttcaacta tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa
      181 gatattcccg gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga
      241 accttcagca gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc
      301 gtatacaatt cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc
      361 ttgttagcag atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca
      421 atggccgaga atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt
      481 gtcttagcta cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag
      541 ctcgatcgtt ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg
      601 ctacttttgg ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat
      661 gaaatagaag aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct
      721 tatgccttgc agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt
      781 ccacgaggaa gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga
      841 aattacgtca cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg
      901 attttttacg atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt
      961 gcacaaattc ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta
     1021 tttcattaat tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt
     1081 caacaattct ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt
     1141 gatttcatta agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt
     1201 ttatcgcaaa aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt
     1261 acctctccct cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt
     1321 ttgattctta caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga
     1381 tgccatgaaa cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa
     1441 agtgcaaaaa gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa
     1501 gcagaaaaat tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt
     1561 cttgattttc gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg
     1621 aaattgtctg ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa
     1681 tgttctcctc ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac
     1741 ttgtattttt ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc
     1801 aaccgcgcct ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc
     1861 gatgagaaag cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt
     1921 gttcctgaga attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg
     1981 gtttatcttg tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt
     2041 tcattgccag gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta
     2101 ctcctttgtg atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa
     2161 cagctacctt cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa
     2221 aaaatgggta cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt
     2281 tccttcgaag acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga
     2341 attcttccat ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct
     2401 cataatgttg ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc
     2461 accctttctt tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt
     2521 actgtacgaa atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt
     2581 tttctccatc cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct
     2641 tatttgtttc ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc
     2701 tctcgaggaa acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac
     2761 actccatgag tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca
     2821 gcttggtgga cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa
     2881 tggctattgg aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca
     2941 ggacgcaccc atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt
     3001 tatagaagac aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc
     3061 ttatggaaat actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt
     3121 gcctgaaagc cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca
     3181 aacagaaagg ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga
     3241 tttagaaaat cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga
     3301 cttggcggtc gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta
     3361 acagtattta aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc
     3421 aaaagaacac gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa
     3481 cttttcaact tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa
     3541 aggttttaca aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat
     3601 tctcaatagt catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc
     3661 atttgaacca accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac
     3721 cgaaaccgtc cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat
     3781 ttccagtgag agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa
     3841 aatcgtctta ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt
     3901 cagaaaatct ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa
     3961 tacgattgca tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc
     4021 agaatctttg caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt
     4081 cattcgatta actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa
     4141 attcgataaa aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa
     4201 aattctgaca aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc
     4261 tggaatgccg agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt
     4321 tttttgtcac aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta
     4381 caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa
     4441 ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc
     4501 agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat
     4561 cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg
     4621 caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac
     4681 ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat
     4741 ctggtaataa accgccataa tattcttcga tccctaaata atctgctact gcttgggcag
     4801 cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga
     4861 taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat
     4921 tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt
     4981 gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt
     5041 ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag
     5101 gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga tgattggcat
     5161 tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta
     5221 cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga
     5281 tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt
     5341 tagctgctat tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag
     5401 gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga
     5461 acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg
     5521 ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt
     5581 ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc
     5641 catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg
     5701 attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg
     5761 ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg
     5821 aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg
     5881 ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta
     5941 gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg
     6001 gattgagcag atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca
     6061 tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta
     6121 aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag
     6181 aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga
     6241 ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg
     6301 aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt
     6361 ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat
     6421 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     6481 tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     6541 tttttcatgt gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc
     6601 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     6661 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac
     6721 aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt
     6781 tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc
     6841 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     6901 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     6961 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     7021 tctaatcctt tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca
     7081 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     7141 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     7201 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     7261 cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     7321 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     7381 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca
     7441 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
     7501 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
     7561 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
     7621 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
     7681 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
     7741 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta
     7801 acttccatgt gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga
     7861 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
     7921 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
     7981 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
     8041 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
     8101 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
     8161 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
     8221 ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct
     8281 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
     8341 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
     8401 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
     8461 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
     8521 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
     8581 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
     8641 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
     8701 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
     8761 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
     8821 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
     8881 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
     8941 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
     9001 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
     9061 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
     9121 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga
     9181 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
     9241 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
     9301 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct
     9361 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
     9421 atcttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
     9481 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
     9541 cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc
     9601 cagataaaag ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc
     9661 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
     9721 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
     9781 tctaatgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat
     9841 tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa
     9901 aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat
     9961 tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg
    10021 gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag
    10081 tcgtgtcaaa gtcccttgtt tcgaataaag gctctataat ttatgggact gatgaatcag
    10141 aattgattct tcagccccat ttttatttta ggtattaaaa aacatatcgt tctctagtat
    10201 gattaaatca cgacaaaata aacaactgag aggacgatat gctatatgaa tgattctatc
    10261 aaaaaaatgc tgcgattaat agagaaagat ttgatgatta cagaggtctc ttacgagacc
    10321 tttcagaaga aaaagacgtt gatcgtcgat gctgttttct cacctgctcc ccatacctgt
    10381 agaaattgtg gttctactgt ggtagatgga aacgggaaag taattgtagt gaaaaatggg
    10441 aagaaggaaa cgattgtccg ctttgaacaa tacaatcaca tgcctttggt tatgcgctta
    10501 aaaaagcagc gctatacctg taaaaactgt cgaacccatt ggacggctca aagttatttt
    10561 gttcaaccca gacattcaat cgcgaatcat gtcagatata aaattgcttc tttactgact
    10621 gaaaaagtat ctttatcttt tattgcgaaa aattgtcagg tatctttgac caccgttatt
    10681 cgtacgttga aagagtttaa aagctactta ccaaagcaat ctaagaggat tctccccaga
    10741 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
    10801 tgcgcagatg gcgaaacagg acaattaatt gatgtcttgc ctacgcgtaa attgcctcga
    10861 ttaacaagct atttcttagg ctgcgctaat cctgaagaag taaaattttt agtcacagac
    10921 atgaacgccg cctacttcca gcttaccaaa cgtattctgc caaatgcgaa agtagtgatt
    10981 gatcggtttc atattatcaa acacatgaat caagccttta atgagttgcg tatccgtgaa
    11041 atgaatgaac ttcgtaaagt aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
    11101 cgctttttgc taaaaaatcg tgcaaacatc aaccattatg aatacaaaac atggaaaagt
    11161 tttcgagcac caaaataccc atttcttacg gaagcgatga tgattgatcg attgcttggc
    11221 ttttctacgt ccctgaagga ggcgtatccc tattttcatg aattagttga agcctttcga
    11281 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
    11341 ggctttcggg aaaagctcca aaaccttctg acctatgaag aaggcatcac caacgcgatg
    11401 atctatcctt acaccaatgg aaaaatagaa gcgaagaata cccatataaa gacaatgaaa
    11461 cgagtatcct acggatttaa atcatttgag aacatgagaa ttagaatctt tttgatcaat
    11521 caatta
//
LOCUS       DARBER010000083        11508 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARBER010000083 DARBER010000000
VERSION     DARBER010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.084"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(110..655)
                     /locus_tag="SCK06_001979"
     CDS             complement(110..655)
                     /locus_tag="SCK06_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1151973.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(667..1359)
                     /locus_tag="SCK06_001980"
     CDS             complement(667..1359)
                     /locus_tag="SCK06_001980"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG1151974.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1378..1662)
                     /locus_tag="SCK06_001981"
     CDS             complement(1378..1662)
                     /locus_tag="SCK06_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151975.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1675..2238)
                     /locus_tag="SCK06_001982"
     CDS             complement(1675..2238)
                     /locus_tag="SCK06_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1151976.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2466..3128
                     /locus_tag="SCK06_001983"
     CDS             2466..3128
                     /locus_tag="SCK06_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG1151977.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3252..4448
                     /locus_tag="SCK06_001984"
     CDS             3252..4448
                     /locus_tag="SCK06_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG1151978.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4549..5925)
                     /locus_tag="SCK06_001985"
     CDS             complement(4549..5925)
                     /locus_tag="SCK06_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1151979.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5962..6828)
                     /locus_tag="SCK06_001986"
     CDS             complement(5962..6828)
                     /locus_tag="SCK06_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG1151980.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6917..7942)
                     /locus_tag="SCK06_001987"
     CDS             complement(6917..7942)
                     /locus_tag="SCK06_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1151981.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7960..8634)
                     /locus_tag="SCK06_001988"
     CDS             complement(7960..8634)
                     /locus_tag="SCK06_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1151982.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8818..9711)
                     /locus_tag="SCK06_001989"
     CDS             complement(8818..9711)
                     /locus_tag="SCK06_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1151983.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9785..11482)
                     /locus_tag="SCK06_001990"
     CDS             complement(9785..11482)
                     /locus_tag="SCK06_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1151984.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3413 a         2522 c         1972 g         3601 t
ORIGIN      
        1 taagtttttt taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct
       61 cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt
      121 ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac
      181 ttgccacggc atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg
      241 tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt
      301 ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc
      361 aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt
      421 cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg
      481 aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact
      541 tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct
      601 ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag
      661 tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc
      721 gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc
      781 acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc
      841 catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga
      901 gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt
      961 tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc
     1021 tggtaattgt cctggtttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt
     1081 gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt
     1141 attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa
     1201 gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat
     1261 aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt
     1321 tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta
     1381 tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt
     1441 catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat
     1501 tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg
     1561 ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg
     1621 cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt
     1681 tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa
     1741 ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc
     1801 gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg
     1861 aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt
     1921 cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc
     1981 ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg
     2041 aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccacc
     2101 aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac
     2161 agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa
     2221 ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc
     2281 atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc
     2341 tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa
     2401 taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt
     2461 ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg
     2521 tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt
     2581 tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga
     2641 ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt
     2701 ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc
     2761 agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt
     2821 tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata
     2881 cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga
     2941 tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt
     3001 cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg
     3061 tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa
     3121 aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt
     3181 tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg
     3241 aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata
     3301 cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg accacttcac
     3361 aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac
     3421 taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg
     3481 cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat
     3541 cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac
     3601 tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct
     3661 tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa
     3721 agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc
     3781 taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac
     3841 tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc
     3901 cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg
     3961 attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac
     4021 agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg
     4081 ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga
     4141 tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa
     4201 caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag
     4261 cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt
     4321 tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc
     4381 atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa
     4441 cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac
     4501 tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt
     4561 catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat
     4621 accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat
     4681 ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc
     4741 aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat
     4801 gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc
     4861 cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat
     4921 aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat
     4981 catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac
     5041 tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt
     5101 atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag
     5161 tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc
     5221 cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac
     5281 accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc
     5341 tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg
     5401 taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc
     5461 aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag
     5521 ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga
     5581 ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc
     5641 accaaataca acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa
     5701 ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga
     5761 cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc
     5821 gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc
     5881 actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca
     5941 ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa
     6001 aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc
     6061 aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc
     6121 aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa
     6181 taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa
     6241 gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa
     6301 attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc
     6361 tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc
     6421 ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac
     6481 ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat
     6541 taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg
     6601 gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat
     6661 ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc
     6721 gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc
     6781 aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct
     6841 tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt
     6901 cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag
     6961 tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg
     7021 ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat
     7081 ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc
     7141 ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt
     7201 ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga
     7261 tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt
     7321 tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat
     7381 attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt
     7441 ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa
     7501 tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat
     7561 ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta
     7621 cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg
     7681 tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc
     7741 cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct
     7801 caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca
     7861 tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt
     7921 tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg
     7981 tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg
     8041 attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg
     8101 cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg
     8161 atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac
     8221 attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga
     8281 acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac
     8341 aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga
     8401 gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc
     8461 caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga
     8521 gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc
     8581 catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa
     8641 tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc
     8701 ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc
     8761 gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta
     8821 tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa
     8881 aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt
     8941 ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac
     9001 tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg
     9061 cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac
     9121 ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc
     9181 ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg
     9241 tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa
     9301 caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat
     9361 ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc
     9421 ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt
     9481 ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc
     9541 aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc
     9601 taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg
     9661 taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac
     9721 cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg
     9781 ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca
     9841 aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc
     9901 cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt
     9961 cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa
    10021 gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt
    10081 gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc
    10141 actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata
    10201 ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac
    10261 tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct
    10321 ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt
    10381 tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat
    10441 ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt
    10501 ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt
    10561 ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa
    10621 ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag
    10681 aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta
    10741 gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa
    10801 ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca
    10861 aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca
    10921 tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag
    10981 ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg
    11041 tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac
    11101 ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg
    11161 cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat
    11221 cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac
    11281 caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt
    11341 ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta
    11401 gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg
    11461 tgacaatcat ttttttattc atttttttct ccccgttctt tttttatt
//
LOCUS       DARBER010000084        11263 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARBER010000084 DARBER010000000
VERSION     DARBER010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.085"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(298..2307)
                     /gene="metG"
                     /locus_tag="SCK06_001991"
     CDS             complement(298..2307)
                     /gene="metG"
                     /locus_tag="SCK06_001991"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG1151985.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2575..2853
                     /locus_tag="SCK06_001992"
     CDS             2575..2853
                     /locus_tag="SCK06_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1151986.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2950..3771
                     /locus_tag="SCK06_001993"
     CDS             2950..3771
                     /locus_tag="SCK06_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1151987.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4056..5066)
                     /locus_tag="SCK06_001994"
     CDS             complement(4056..5066)
                     /locus_tag="SCK06_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1151988.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5078..6031)
                     /locus_tag="SCK06_001995"
     CDS             complement(5078..6031)
                     /locus_tag="SCK06_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1151989.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6021..7091)
                     /locus_tag="SCK06_001996"
     CDS             complement(6021..7091)
                     /locus_tag="SCK06_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1151990.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7097..8140)
                     /locus_tag="SCK06_001997"
     CDS             complement(7097..8140)
                     /locus_tag="SCK06_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151991.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8141..9082)
                     /locus_tag="SCK06_001998"
     CDS             complement(8141..9082)
                     /locus_tag="SCK06_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1151992.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9166..9513
                     /locus_tag="SCK06_001999"
     CDS             9166..9513
                     /locus_tag="SCK06_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG1151993.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9583..11244)
                     /locus_tag="SCK06_002000"
     CDS             complement(9583..11244)
                     /locus_tag="SCK06_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1151994.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3164 a         2300 c         2055 g         3744 t
ORIGIN      
        1 ttcaaacttt atgaatgtaa ggacaataaa aaagagtacc agatgaatca attaaaatct
       61 gcccgaccct ttatcattat gctttttttc tgtttttacg attcctatta gtattattct
      121 aaattaacaa aaatcagcga agctcgtact tctttttcta ggatttattt tggatagttc
      181 tctgtttctg tattatatga ttggcttcaa gtaataagaa ggaatttacg aaaattgctt
      241 ctcaaatttc ggaaaaaggg ggtatgacac aactagtgtc ataccccctt tgaatttcta
      301 tgcgatttct gagccatttg gcatggattt tggtgcttcg attacttgta aagagccatc
      361 tggtgcttct gcagaaagga tcattccttg actgatttgt ccacgcatct ttcgtggttt
      421 cagatttgct acaatgacga cttttttccc gatcaattcg cttggatcag gataaaattc
      481 agcgattcct gacaagattt gacggtcttg gctgtctcca gcatctagtc ggaattgcag
      541 caacttatct gccccttcta ctttttgaca gttgatgact tctgctactt ttagttctac
      601 tttttcaaat acatcaaatt tgatttgttt ttctttcgtt gaaacaagtt ctgtttcttc
      661 tggatcccat ttgactgtat cttcgtttgt ttgagtacct tcagacattt tttcttgaat
      721 aaaagcaact tcttcttcca tatctaaacg tgggaagatt ggtgtacctt ttgcaactac
      781 ttttgttcct gaaggaaatt cgccaaagtg taatccttca agattcattg tttcagagtc
      841 caatcctagt tgattgaaga tcttctctgg cgtttctgtc atgattggct gaagtaatat
      901 cgctgcgata cgcaaagatt cagctaaatg gatcatgaca ctattcaatt cagctttctt
      961 ttcttcgtct tttgctaata cccatggttc tgtttcatca atgtatttgt ttgcacgaga
     1021 aatcagcacc cagatttcag ccaatgctgt attgaattcc attttttcca ttgcatcatg
     1081 gtaacgtccg acaacgttag cagccgtcgt agacagttca ctatcaaatg gagtgacaag
     1141 tgaggcatag tcaggaacga gtccatcaca atatttgttg atcatagcaa tcgttcgatt
     1201 caataagttc cctagatcat ttgccagatc ataattgaca cgggaaacaa aatcttctgg
     1261 cgtaaagaca ccatctgacc caaacggaac agcacgaagc aaataataac ggagtgcatc
     1321 taaaccataa cgttccacaa gcatttcagg ataaacgaca tttccttttg atttagacat
     1381 ttttccgtct ttcatcaata gccagccatg cccaaatact tttttaggta gaggcaaatc
     1441 aagggccatc agcatgattg gccaatagat cgtatgaaag cgaacgattt ctttaccgac
     1501 catctgaaca tctgcaggcc agtatttttc gaatagactt tcatcttcgg agccatatcc
     1561 tagtgcagta atataattgg ataatgcatc gatccataca tacaccacat gtttcggatc
     1621 actcttgact ggtactcccc aagtaaaagt tgttcgtgaa actgcaagat cctctaatcc
     1681 tggtttgata aagttgttga tcatttcgtt tttacgagat tctggctgga tgaattcggg
     1741 atgttcttca tagtattcca atagacggtc tgcgtatttg ctcatgcgga agaagtatga
     1801 ttcttctttt accaattcta cttcgtgtcc acttggcgct ttaccaccga tcacgttacc
     1861 ttcgtcatct ttgtacactt ctgcaagttg tgtttctgta aagaattctt cgtcagaaac
     1921 agagtaccag ccttcatatt cacctaagta aatatctccc tgttctaata aacgatcaaa
     1981 tattttttga acagctgctt tatggtaatc atctgtcgtg cgaatgaatt tatcataact
     2041 gatatcaagc gttttccata atttcttgac atcagccgcc attttatcta catattcttg
     2101 cggttttaca cctagttctt ctgcttttgt ttcgattttt tgaccatgtt catccactcc
     2161 agtgagataa aacacatcaa agcccatcaa ccgtttataa cgagcaattg cgtcacatgc
     2221 aatcgtagta tacgagttgc cgatatgaag tttgccgctt ggataataga ttggcgtagt
     2281 gatataaaaa gtttcctttt ctgccacggg gctcttcctc ctttatcgca tgatgcgaat
     2341 tcgtttcctt gctagtatac cataaaaaaa gcatgctgtt agcttttttt caaaatgaaa
     2401 aagactggat aaacgacttc tatctacgtt caatctatcc tttaaaagga tgaataggtt
     2461 gaataaatgc gggtttttgt tttgtttcat atctactaaa aaacggcttt ttatttctcc
     2521 cctcacacgt ggtataataa agactatcta tcttagtaag ggactggaac aatcatgcag
     2581 aagaaacatc ttttttttac acttagtatt gcttttcttt cattagcaca tttaatattc
     2641 agctattttt atattcgtat gtatggttat tttaacctcc atgggcattt gaacagtttt
     2701 atgacagcag cttggatttt gcgttttatc attgacgtat atatcgttat ttgtggcttc
     2761 tttgccattc gtgaagagcg gtataaagta ttgccgtttt atctgctttt ctttttattc
     2821 aatctcatac ttccatttat ttttcatatt taaatgataa cctagatttt tcaagtaaat
     2881 ccttggtttt ttttcagagt ctgctacaat aaggtaaaaa gtacttttac tgtcaatgga
     2941 ggtggtcaga tgaaccgttt tgcttctaaa gaagaagaaa aattatatta tgaacataaa
     3001 gcaagtgaag aagaatttct taactggtat caaaaacagg aacgtccaga atatgacaag
     3061 ccctctttga ctgtcgatat cgtactgatg tgctataaca aagaagaaga tcagctgaaa
     3121 gttttgttga tccaaagaaa agggcatcct tatcgaaatt catgggcatt gcctggtggc
     3181 tttgtccaaa aggatgagtc aactggagaa agcgtcttaa gagaaacaaa ggaagaaact
     3241 ggtgtggtta tttcaaaaga aaatattgag caactgcata cattcagtac gcctaatcga
     3301 gatccgcgag gctgggtcgt cacagtcagc tacttagcct ttattggaga agaaccgcta
     3361 atcgccggag atgatgccaa ggaagtacgc tggttcacac tagaacgtca tagtaacaag
     3421 atccatttat ctagcggaga agtggctatc tcgcttgatt tagtcactgg agagtcttct
     3481 ggcaaagata ctttggcttt tgaccatagt caaatcatct tgaaagcatt caaccgtgta
     3541 gtcaataaaa tggaacacga gccgcaagtt ctacaagttt taggtaaaga tttcacgatt
     3601 acggaagctc gaaaagtatt tgctaaattc ttagggatcg attataaaac gatcgatcat
     3661 tccaatttta agaaagctat gctccaatat ttcgatgaga tcggtgaacg tcctgtagga
     3721 atcggccgtc cttcaaaaat ctatcagctc aagccggggc accatgaata aggttgtgag
     3781 caaaagattc agacctaatc attcaagaaa aaacgcaaaa acaactcttt gtgagttacg
     3841 ttttttgttt cagaattttg atgtattcgt aaactttctg aataaaaaat taacaatagc
     3901 agaaaaagct cgtacgaact ttttctgcta ttgttttatt ggtttgtcat aattagtgag
     3961 cttttcttca aaagaaaaat tcctagaaac aaaagtcatt aagacttttt attaacaagg
     4021 ctaaccctaa cgctcctatc agtttcattc attttttatc ccttgataac cgaatgtctg
     4081 cccatttttt ctactacttg gctctttctt acggaattgc tttcttctgc tccgtcggta
     4141 ttgatcaaaa gaaatttttt attataccat tgggacttat aacgcaattt tgttattaat
     4201 ttatgccagt cgtgaataga aaaatcagca tgtgcctctt cttttggaaa agattctaca
     4261 aataagtagt catattgttt gaccaatttc ccagataatt gttcaagata atcttctcgc
     4321 tggttctttt gtttcagata aagatccatt acttttctct tttgtttctg ataattgctt
     4381 gcctgatcta acctgatctt tctatggtga gcggagcgga ctttcacatt caatttccgc
     4441 tgtgcatgtt gcagcttttc ttctgtctgg tgaaattttg gtaaagttac ttctagaggt
     4501 cgagatgttt ggatcaatgt cttcggatga taggcgatcc cgatccattt agctaaagat
     4561 tcttttttta ctggcgaaac atcacaaagt agtgcgacat aaaattcttc attatatcga
     4621 gcagaaatcg tagcggagcg aatcgatcct tcaactggcc gatgctgatt tatcttgatt
     4681 agttcttttt gttttggtaa ctttaagtag ccattttcca gatagatcgt acgggtttgg
     4741 ttgttggtcg tataagattg ccacatactt tttttcgttt taagctttgg gaagctggca
     4801 cgtcccttga aataattgcg aaaagcccga tccaaattaa gctgtgcatt tgctaaagca
     4861 agactgtcta cttcttttaa gaatgggtat tcttttttta atgtagcggg tgtgagcgaa
     4921 aaattggcta ctcctgtttg ttgccaactt ttctgccgct ttttcagcaa atggttatag
     4981 gtaaatcgag cacatccaaa agtacgaatc aattgctgtt tttgttcttc tgtcggatac
     5041 atacggaatt taaaagcttt tagcatctat gtccctccta gttagcaacc agaagagatt
     5101 ccttttgtcg ttttagtttt tttgcgtaat atgggacttc ctgctcagtt gcatagacaa
     5161 aatgttctgg tgcgatttca cgaagttgac gtacttgtcc gtcatctggt tcaggttgat
     5221 atttgattct tctgcgtttt ctctcatggt ctggatctgg tagaggaata gcagataaca
     5281 aactttctgt ataaggatga acaccgtgat tatagatttc atcacttgtt cccatctcta
     5341 ataacaagcc gttatgcatt acaccgattc ggtcactaat atgtttaacc atcgacaaat
     5401 catgtgcaat aaatagatag gtcaattgat gttctttttg gagatcttgc aacaaattga
     5461 ccacttgggc ttggatcgac acatctagtg cagaaattgg ttcatcacaa atgataaaac
     5521 gcggttttac tgccaatgct cgtgcaatac caatacgctg gcgttgccca cctgaaaatt
     5581 catgaggata acgagtaccg tggcttggat tcaatccaac agttctcaat agatcatcga
     5641 ctttttttgc acgctcttca gatgatttag ctagtccatt aacatcaatt ccttcggcaa
     5701 tgatatctct gactttcata cgaggattca acgaagcgta tggatcttga aagatcatct
     5761 gaacatcatg ccggaatttc gtcaaacctt ttttatcttt gatcttcgtt atatcttgac
     5821 cgtcgaataa tatctcacca tcagtaggtt cattcaagcg aatgatcgta cggccagtag
     5881 tggattttcc gcttccagat tctccaacta aaccgaatgt ttctccttca taaatgtgaa
     5941 agctgatgtc atttacagca tgtacttcat tcttttttcc tacattgaaa tactgtttca
     6001 atcctttaac ttcaagtagt ttatttggca tcagcaattc ctccttgacc gtaagtatgt
     6061 ttttgtttct ctttaaagat agcccagcga cgttggattt cttctggcgg tgtcactttg
     6121 ggtgcctgcg gagcaagtag ccaagttgct gccttgtgtg tttttgatag ttcaaaaaat
     6181 ggtggttctt gttctgcatc gattttcatc gcgaattcat ttcttggata gaatgcgtct
     6241 ccttttggcg ggttcaataa atccggtgga gaaccaggaa tagcatacaa tcgatcgttc
     6301 gcactatcta atgtcggcat tgaaccaagc agtccccaag tatagggatg ttgcgggttg
     6361 tagaaaattt cttccgatgt tccaacttca acgattcttc ctccatacat gacagcgaca
     6421 cgatcagcga cattcgctac tacaccaagg tcatgagtga taaagataat tgatgtatcg
     6481 atttttgtct gaatttcttt cagcagctcc aaaatctgtg cttgaatagt cacatcaagc
     6541 gctgttgtag gttcatcagc aatcaggatt tccggataac ataccagtgc aatcgcgatc
     6601 acgatccgtt gacgctgacc tcctgagaac tgatgcggat aactgttgat acgtttctca
     6661 gcttcgggta ttccaactaa tttaagcaat tctaatgcag ctttttgtcc ctcttttttt
     6721 gaaactttat tatgctttct aagggactca gcgacttgct tcccaatggg catcgtcgga
     6781 tctaaagagg tcattggatc ttggaaaatc atcgctattt ctttaccacg gattttttgc
     6841 atctctcttt ctgattttcc aacgatatcg ttacctttaa agaggatttg accagcatct
     6901 atattggcgt tgcttgacag caagcgcatg atgcttctgg tcgtcactga ttttccactt
     6961 ccagattccc caacgatcgc caatgtttct cctttattca aatgaaaatt gacaccgcga
     7021 atcgctcgta cctttccagc atacgtatca aaagagattt ctaagtcttt cacttctaaa
     7081 attttcgtca tcgtcttcac tctttcattt taggatcaaa ggcatcacgc aatccatcag
     7141 caagtaagtt aaagccaatc atgataaccg ataaagttac tgccggaatc cataatagat
     7201 aaggcagata caggaatgtc ttgtaccctt cgctcaacat cgttcctaat gaagctgacg
     7261 gaggcgtaag acctaaaccg ataaagctta aaaaggcttc gaagaaaata gcagatggaa
     7321 tactaaacat catttgaata ataatgacac tagagatatt cggcaaaata tgtttgaatg
     7381 caattttcat tcttgattca cctaatgtca atccagctaa aacatattct tgatctttta
     7441 atttcattgt ttgtgcacga acgatacgtg ccattggtat ccagtttgtg atggccatag
     7501 cagccacgat tgaaaaaatt cctggttcaa aaacgaccaa catcaaaatc atgacaacga
     7561 ggtttggaat tcctgataag atttccaaaa aacgttgcat cacgttatcg acacgtccgc
     7621 ctaacaaacc agaaaccaac ccgtaagcta caccaatcgt tacatctagt aatgcagcaa
     7681 taaatgcgat cagcaacgag attctcattc ccataaataa gcggcttaat acatcacgtc
     7741 ccaaaccgtc tgttcctaaa aagaaattta cgtttgaagg gacgtttgct tgagcatatt
     7801 tatcaacaag ttcaccacca accattgctt ttccattcaa tccatcgata ttgattcccg
     7861 gaatacgagg aggcaaattg atataagcca cgttttgggc agttggatca tgaggagaaa
     7921 caaagattgt gatcacagag atcaaaatga tgaccaatag taaaaccatt gaaataacgg
     7981 cagctttgtt tttctttaaa cggcgccatg aatcttggat aaaattcaaa gaaggtgcag
     8041 taatctgttc acgttcttct tcggtatttt tttgtaatgg aagaaattca tccgcaggga
     8101 tatctgcaac tgtttcataa cgctttggta tcatttccat ttatcctcgg ctcccttctg
     8161 aaacacgaat tcttggatca actagaccgt acaatagatc cacgatcaaa ataacaaaaa
     8221 tcagcataaa tgagtaaagg atcgttacag ccatgatcgt aggataatca tttgttgtga
     8281 ttgacttaac gaattgttcc ccgataccag gaatagcaaa gatattttct accaccagtg
     8341 atccggtcat caatgctaca gctaacggtc ctaataaagt catcaatgga atcaaactat
     8401 ttcgaagacc atgtttaaag gcgatttccc aacggctcaa tccttttgct tttgctaatt
     8461 ccacataatc actatgcagt acttccacca tttccgtacg gataaaccga gcagagtcag
     8521 caagcggcga catggccaac gcaattgttg gtaagattgt gtacacaaag ccatcccatt
     8581 ttgcgattgg caaaacttgt aatttgattg caaagatata ttgtaataaa acagcaaata
     8641 caaagtttgg aatggaacga cctaatatcg ctacgagtgt agaagatgta tctgcccatg
     8701 tattttgctt catggcagag atcgttccta aaatcgtccc taggactgtt ccaaagataa
     8761 ttgcctgtaa ccctaactgc aaggatggtc cgattcttcc agctaacaaa tgtgcgactg
     8821 gctggttttt aaattggaag gaaattccaa aatctccttg aagcaaatta gaaagataaa
     8881 tcccatattg tacgataact ggtttatcca aaccgacttg tttatttagc atctcgattg
     8941 tttcaggact caaacgttcc tgattcgtat atggcgtgcc cggcagcaat tgcatcaaga
     9001 aaaaggtgat tgttgcaatt aaccaaagcg tgatgatcat gaaaaagaca cgttttaata
     9061 catatttgat ataactattc actaatgttt ccccctagct tttgacttct gacagatgtt
     9121 tcgctacatt attagataat gaaccaaaaa taaaggagca atcaaatgaa aaaaaaatct
     9181 atcccctacg cagtagcttt tctactgatt ctggttattt taataaaaaa tctcatcaac
     9241 cattccttta cactcattca attatctaat gatctattct tatggtcatt gccgtttctg
     9301 attatcggcg gttttttatg ggtcttttct tcgggtttct tcgatcattt tcagcgatcg
     9361 gtccatcttg cacgaacaag gaaccgaaag aaaaaaccag aattttcctc gctctcgtct
     9421 gccagctacg gtatgtacag cttttggctg atcattgccg gaatactaat tgctctttct
     9481 gctattttca tgcttttttc tttgttagga taagtgaata tgagaacgag gttgcgacat
     9541 ttgccgcagc ctcgtttttt ctcatgctca cggtattaac gattattctt caacgtatgt
     9601 ccatttgtaa tcccatgaag cgcctgccgc atggtgaacg atacctttga ttttttcatt
     9661 cactaggtga ccttctgctt tttggtacac cggaacgaca cccatatcat cagagatgat
     9721 cttgattgct ttttggaaat catcccaacg ggcttgttca tcgccagcgt ctgttgatga
     9781 agctgcttta actgcttcgt catattctgc attgctccag cgaccacggt tgtatgaatt
     9841 acctgtgaca aacaaatcag taaagctgct tgggtctgca tagtcagcag cccatccgcc
     9901 tagtactgcg tcaaaatcac ccgaagttga gcggtcaaga cgtacagaga atggaacagg
     9961 tgttggtttg actgttacgc catccaagtt ttcttggatt gagttttgaa catattcgat
    10021 tacttttttc gatgaatcat catcagaagc catcaaatca aattctaaag aatcaacgcc
    10081 caattctttt ttcgcttttt cccaatattc ttttgcttta tctttgtcat aagaaactaa
    10141 ttttccagct tcttttgcaa agtcttcgtt cgttgttgga ctttttacca tatctgatgg
    10201 gatcaatcct gtagagacaa ctgatccgtc acctagtact tgttttacta aagcttcacg
    10261 atcgatagaa taagaaatag cttttcttag gttcacattg ttgaatggtg aattcttttc
    10321 acgttgattg aattcgatat agcttgtccg cgcttcttta acagaagtat aagctggatc
    10381 gtttgcgttt tgctgtgcta actcaccaga caagatcacg tcatctgctt ggccatcttt
    10441 gaataagttc aatgcagtag aagattcttt tactacactg actttgattt cagaaagttt
    10501 tactgtatct ttgtcccagt aatgatcatt tttcttataa gtccattctg tatctgttcc
    10561 tggtccatca aaatcttcta atgtgaatgg tccattataa acagctttat cacttgcaga
    10621 tgcataggca tccccatttt cttccactac ggattgtttt tgtgggaaaa atgatgggaa
    10681 agcaagcaga tattggaaat atggtgtttg tttcgtcaat ttgatttcta attcatagtc
    10741 accaactgct ttgatcccta gttctgattt atctttcttt ccagctgtaa tgtcttctgc
    10801 attttcaact ggcgcataca ggtaagcata ttcagaagct gtagctgggt ctactgttcg
    10861 ttgccagcca taaacgtaat ccgctgctgt aacaggatca ccatttgacc attttgcgtc
    10921 ttctcgtaat ttgattttgt aagttaatcc gtcttcactg acttctgcca actcgctagc
    10981 tcctgccggc tgtggtttgc tgtcttcatc aagacggtaa attccttcat aaacgttatt
    11041 caatgctgta aaactgattg tatctgttgc aactgataag tcagctgtag gcatttcttg
    11101 aggcacgact aagttaaata cgcctcctcc atctgctgaa ccgctttttg ctgaactact
    11161 gcttgtggct gaactgccgc cataacatcc agcaagtaca aggccgcata ccgcaaccaa
    11221 accaaatgta aatttctttt tcattttacg taccccttta ttg
//
LOCUS       DARBER010000085        10928 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARBER010000085 DARBER010000000
VERSION     DARBER010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.088"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..287)
                     /locus_tag="SCK06_002001"
     CDS             complement(<1..287)
                     /locus_tag="SCK06_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1151995.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="SCK06_002002"
     CDS             429..1547
                     /locus_tag="SCK06_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1151996.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="SCK06_002003"
     CDS             1675..3051
                     /locus_tag="SCK06_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1151997.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="SCK06_002004"
     CDS             3452..5032
                     /locus_tag="SCK06_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG1151998.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="SCK06_002005"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="SCK06_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="SCK06_002006"
     CDS             complement(5658..5978)
                     /locus_tag="SCK06_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG1151999.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="SCK06_002007"
     CDS             complement(6126..8360)
                     /locus_tag="SCK06_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1152000.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="SCK06_002008"
     CDS             8839..9105
                     /locus_tag="SCK06_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG1152001.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="SCK06_002009"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="SCK06_002009"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG1152002.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2215 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaa
//
LOCUS       DARBER010000086        10913 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DARBER010000086 DARBER010000000
VERSION     DARBER010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.089"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            54..1205
                     /locus_tag="SCK06_002010"
     CDS             54..1205
                     /locus_tag="SCK06_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG1152003.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            1336..2055
                     /locus_tag="SCK06_002011"
     CDS             1336..2055
                     /locus_tag="SCK06_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1152004.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            2234..2881
                     /locus_tag="SCK06_002012"
     CDS             2234..2881
                     /locus_tag="SCK06_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152005.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(2974..5253)
                     /locus_tag="SCK06_002013"
     CDS             complement(2974..5253)
                     /locus_tag="SCK06_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1152006.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            5531..7354
                     /gene="cadA"
                     /locus_tag="SCK06_002014"
     CDS             5531..7354
                     /gene="cadA"
                     /locus_tag="SCK06_002014"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1152007.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            7401..7580
                     /locus_tag="SCK06_002015"
     CDS             7401..7580
                     /locus_tag="SCK06_002015"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG1152008.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            7917..9857
                     /gene="thrS"
                     /locus_tag="SCK06_002016"
     CDS             7917..9857
                     /gene="thrS"
                     /locus_tag="SCK06_002016"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG1152009.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            10081..>10913
                     /locus_tag="SCK06_002017"
     CDS             10081..>10913
                     /locus_tag="SCK06_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1152010.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     Y"
BASE COUNT         3498 a         1932 c         2350 g         3133 t
ORIGIN      
        1 ttttttactt gtctctattt cttttttatc gataactatg aaggaggatg cgcatgaaaa
       61 gctgcgggat tatcgtagag tacaatcctt ttcacaatgg acatcgctat catgtggaaa
      121 tggcacgaaa aataacagga gcagaggtcg tcattgctgt aatgagcggg aattttttgc
      181 aaagaggcga accggctatc atcgataaat ggcatcgcgc gaatgaagct ttacaaaatg
      241 gtgtggattt agtagtggag cttcccgtcg aatgggcggt tcagtcagct gactattttg
      301 ctaaaggtgc agtaaaaatc ttgcaaacac tggactgtga cagtttgtgt tttggaacag
      361 aagaagagac ttcgtttgat tacagcagtt tcggacgctt tgtccaagag aatcaagata
      421 ggatagaaga ggcatttcaa gaactgtctg atcaaacgta tagttatccg caaaaaatga
      481 ctgaggtttt tcgcagtctg tatcccgata ttacgcttga tttttcttcg ccaaatcata
      541 tcttgggatt aagttatgca aaggaaaatg ccaagtatgt ccaccctatg gaattacatc
      601 cgatccgacg taaaagtgca ggctatcatg aaaaaatcat cacagatcag aggatagcca
      661 gtgcaacagc cattcgccat gcgttagaag aaggaaaaag aatagctgga cttgtgccgc
      721 ctcagacaga agaagatctt tcaacttgtt cgctgcagac ttgggaatct tattggccgt
      781 atttgcgtta tcggttgtta tcttctactt tgctggaact gacagatatc tatcagatga
      841 ccgaaggaat agaatcgcgc atgaaaacag cagctgcaac tgctgatagt tttgaggatt
      901 ttgtgtcaaa ggtaaaaaca aaaagatata catggacacg gatccaacga ttatcttgtt
      961 atgtcttgct gaatatcaaa aagcaagaga ttcctgtcca acaaaataaa aattatcttc
     1021 gactacttgg atttaccgat gctggaagaa gattcttaaa agaaaaaaaa gagatttcta
     1081 tttattcccg aatcggaaaa aaagaggcac aagaagcgga actgttagtt agaagtgatc
     1141 aaatctatca attgggaggc aatattccgg aacagaattt cgggcaaatc ccaatccgag
     1201 tatagataat tgactaaaaa tgagaagaat gaaggaagaa agaaaaaaag cttcacagaa
     1261 tcgtgattca caagtataat ggaacaggac gtttttgcgt tatgaaacta tcaaatcaga
     1321 aagcgaagtg aaaatatggg acgtaaatgg gcaaatatcg tagcaaagaa aactgctaaa
     1381 gatgctaata atagccggat ttatgcaaaa tttgggattg aaatctacgc ggcagcaaaa
     1441 tcaggtgatc ctgatccgca tgcaaaccaa aaactacgtt tcgtcattga tcgcgctaaa
     1501 acttataatg taccaaaaca tattattgac cgagcaatcg aaaaagcaaa aggtgcagga
     1561 gatgaaacat actctgaatt gcgctatgaa ggatttggac caagcggctc aatggtgatt
     1621 gttgatacat tgacgaacaa tgtgaatcga acagcagcag acgtacgggc agcatttggg
     1681 aaaaatggcg ggaacatggg tgtttccgga gcggtttctt acatgtttga caatacagca
     1741 atctttgctt ttgcaggaga agacgctgac gagatccttg aatacctaat ggaaaaagac
     1801 atcgatgttc gcgatgtaat ggaagaagaa ggacaaatca tcgtttacgg tgaagtagcg
     1861 gatttccata gtatacaaga agcattaaaa gaaaaaggaa tcactgattt ttcaatcgct
     1921 gagatccaaa tgatccctca aaacgaggta acactatctc cagaagatca agaaacattt
     1981 gaaaaaatga ttgatgcact ggaagatcta gaagatgtac agcatgtgtt ccataatgtg
     2041 gcattagaag actaagcaac cagttttaac gaaaagtatt cagtcttttt cgaaaagcaa
     2101 gacaatctca ctaacagatt gtcttgcttt tttgtgtgat ttattttctt ttctaattca
     2161 acctgtggta tgatgagagc ataagtatca tgtgaaatga tatgaagtat gtggaagatg
     2221 gacggggaag aatatggaag caaaaatcac tttagaacct tttgaacgta tcttaagcgg
     2281 ttaccgcaaa gtagaagagt tagctgtgaa cgtaactgat tgttcaaaat tagcgcaaaa
     2341 atacgcaaga ttcggcgttg aaggctatcg attaggaaac tatgtcggta cagggtatct
     2401 gaaccgttat ttagagtgta tggtggaccg cgcaccaatg ctgatttata gacaaaatta
     2461 tctgattccg ctgctttttc gtcgttctga ttcagccttt cggttatttg aagaagagta
     2521 tcggatggag gctttctttc tactattaga gtggagcttg aaacatcgtc ctgaaaaaat
     2581 tcttatcgaa agaaatgaga agatagacac gaaaaaaaat aaagtgatcg atagtgcata
     2641 cctagcattt cgtgtatcag agattttgga ttgtggcggc tatccaatca gcaatttcca
     2701 atcaattgac caattcattg aatggaatcg aatttaccga ttgatcgaca atggcgggat
     2761 tggccgtcat agcaaggtat ttgacccaga atatccagaa aacatggaag aactgaaaat
     2821 gattatttcg cttgtcaaat taaaatatcc tgaaacagat ttggatttat atatagaata
     2881 aatgacctta aatgattgaa ttactttttc tgcaattatt attgtaaata aaaagacaac
     2941 tgctaacatt aatgatagca gttgtctttt tacttacagt acttttttct cataagatga
     3001 aatatccata ttgtttaaca aaggactttc attattggag gtaaaaaggg tcaattcatg
     3061 catcgcacgt gtacacatgg tgtaaaggat caatttgtct tgctgagaag aaaaatgttc
     3121 tgcatctaca tcccacaaga aaactcggtc aaactccaga ccttttgcta aaaaggctgg
     3181 aagtaaaata atggaacgct tcataaagtc actctcattt aaaatcagct ggacttgttg
     3241 tttcaactcg tctggcagtt gagcataaag agcttcgcat tcttcttgtg ttttagctat
     3301 gattgctgta cgccaatacc gggcttttcc ctgagtcgtt cgaatggtat ctgctaacca
     3361 attcaatttg atatcatctg ttgttccttg gatgagcaaa ggaaggtctc cttttcttgc
     3421 tgttgtttct acttgatgtt ctttcacaat aaaatgatta gcaaaatcag tgatttcttt
     3481 tgtggagcga tagcttgtcg ttaattggaa acgagtgact tcacggtcca cgaaaaggtc
     3541 ttccaatgat ccaacgatcg tttcatttcc aaagacattt tgatttagat ctccacagaa
     3601 agtcaaattt gcttttggga acaaggcttg cagcaatgcc acttgtgatg gtgcgaaatc
     3661 ctgcatttca tcaataaaga tatagcgagc tttgtgtgta accgtcactg gatatgttcg
     3721 gcgcatcagt aaaaagaata gtacagcatc ctcttgacgc aattctcttt gacgcaagtg
     3781 tgtacgaaca gttgtcaaat gttcctgcca cctggtctcg ctgattttat gtcgtgttag
     3841 aagagaaagt ggcacagttt gtagaaagtg gagatattgt ttcgttatat tgacaaattg
     3901 ataattcatg atccctcgat ggattcgacg gaatctttta cgtacgattt ggttggccag
     3961 ttttttacgt aatgaacgct ccttttgttc tgtatattca agatttggat ctgttgcgta
     4021 tagttcttgg agctgttctt ccgccagatc tttgacccat tgctgacgtg tttcgtcttt
     4081 ttgcagaccg cctaattttt tcaggagctt cgtttgtaat agagaaagtc tatgatgtaa
     4141 aggtaattgt tcatttgttt ctttatacca ttggcgaatc gattcctttg ataggatcgt
     4201 acggccattt attttcatat cacggaacaa gggaccgtag tttgtgatgc cttggatata
     4261 gcgatcaatc tgatcgacta aggtcaatcc gcttttcatc acttgtatcg gatctttttc
     4321 ccctagcaaa aaaccacttt cttgttgctg ttcatctagt aaagaaaact ctggcagcag
     4381 ctgcaccaaa taatttttga atgtctgtgt aggcacgccg ctttcaccta atgaaggcaa
     4441 tacagtcgag atgtaatcag aaaacaaatg gttgggtgaa aatagaagaa cgttttcagc
     4501 ctcgagccac ttgcgattat gatacaacag aaaagcaata cgttgaagca aagcggatgt
     4561 ctttccactt ccggcgattc cttcaatcag cattacttta cttgttgtgt cccgaatgat
     4621 tgcattttgt gctttttgta tcgtagagac gatgtttttc atatggttgc ttgatgattc
     4681 atccaatatc tctagcaaaa agtcatcatt gattacttcc gacgtatcta ccatagataa
     4741 aatacgtcct tcttggattt taaattgtcg tttcaactgc agatcgacat ctattctatc
     4801 tgtttcagtt tcataaaatg cgggaccaat ctcaccttca taataaagat tggcaattgg
     4861 tgcgcgccag tcaataacga ttgtttcttc tgccagatct cttaaagaag cgatacctag
     4921 atataatgtt tctgtttcac ggtcttcttt aaagtcgata cgggcaaaat aaggactatc
     4981 tttcatgaca gacaacgttt gcaggcgttt ttgctgtgct tcaagagtat ggtatttcaa
     5041 aagcaattcc tgctcatgtt ggcgatattc aacagcagat tcataaaaag cttcattgga
     5101 accagctcga atcttgtgat tagctgtgtc ttttgtcgaa tgattcattt cctgagtcaa
     5161 ttcttgttgt tttgtatcca gcatattttg ttcgatctgg attagttcga tcgtttcatc
     5221 cacgtgcgtt tgttctagtt gtttctcatt catcgcatgt ttgctccttc cgccgtaaat
     5281 aacgaattaa aagtatagca tagtttttcg aaaaaaggta ctttttaact gatgaatcga
     5341 aaaagtaatc cttgctgaat atttttgttt gtagaactga tgaattttgg aaacgctgat
     5401 agtgatagtt gttttcaatt aaggcctatt gttttaaaaa gtgctagtca aggactatgc
     5461 ttaagatgaa agaggcagct ggaaatttat tactaacctc tctaagtcac ataaaagagg
     5521 agaaaaaaca atgacacatt tcaaaaaatt tttacttact ttgcttattg gtgggatagc
     5581 tttattaagt gaatttgttt ttcaccagcc ggcccttgca tttgcgattg tcgcatttac
     5641 tggagggatt ctcgccttct tgatgttttt ggaaatgatc aagacgttac gttcaggaag
     5701 atatggcgta gatgtattag cgattaccgc aatccttgca acattagccg tcaaagaata
     5761 ctgggctagt ttgatgatat tgatcatgct tacgggcgga gatagtctag aggattacgc
     5821 gaataaacga gcgggtaaag aactgcagtc tttgttagat aacaccccaa gaattgccca
     5881 tcgattcaaa gaaggaaaat tgcaggatat agtaatcgat gaaataaata ttgatgacca
     5941 gttactagtc aagcctggcg aacttgtacc cgcagatggt cgagtgacca aaggagaatc
     6001 ttcggtcgat gaatcttctt tgacaggtga gtcccgcccg gaaaccaaga aacctggaga
     6061 tcagattatg tcagggtctt taaatggaga cggatcgatc caaatgatcg ctgaaaaacg
     6121 tgccgcagac agccagtatc aaatgattat taaactagtc gaagaatcaa aagaaaagcc
     6181 tgcccgattt gtccgtttag ctgatcgtta tgcagtccct ttcacgttga ttgcttattt
     6241 gatcggtggt atttcttggt ggttctcaaa agacccttta cgatttgctg aggtactggt
     6301 agtggcatca ccttgtccac tgattctagc tgcgcccatt gctttagttg caggaatgag
     6361 ccgttcaagt aaaaacggga tcgttgtaaa aactggaaca acgattgaaa agctcgcacg
     6421 tacaaaaacg attgcttttg ataaaacagg aacgcttacc aaagggatat tggaagtaga
     6481 ggcgattcag ccagtcaatg gaataaaaaa agaagagcta cttctgtatg cagctagtgc
     6541 agaacaggaa tctagccata tcttagctcg ttcgttagtg aacaacgtga aaaacagcca
     6601 actgtatcct gttagtcaac tgaaagaagt gaccggtcaa ggaatccaag cagtagtcaa
     6661 taatcatgaa gtaaagattg gacgtgcctc ttttgtttca gctgaagaaa tagaaacgtc
     6721 caatacagca atctatgtaa caattgatcg atcttttgca ggaacgatcc tattttcgga
     6781 tcagattcgc ccagaagcag caaagacaat caaggagcta agaaaacttg atgtgaaaaa
     6841 tttgatgatg atcaccgggg atggatctgc cattgctcaa acgattgcag aaaaagtcca
     6901 attagattct gtacatgccc gtagcttgcc tcaagataaa ctgaccattt tagaaagtat
     6961 ccctaaagat caaagacctg ttgcgatggt aggagacggc gtgaatgacg caccagcact
     7021 tgctgctgca gatatcggta ttgcaatggg ggctcatggt tctacagctg ctagtgaaag
     7081 tgcagatgcg gtcatcttga aagatgatct cttgcgtgta gcaaaagcag tagtgatttc
     7141 aagagatacg atgagaatag cgaaacagtc tgtactgatt ggcatcttta tttgtatttt
     7201 tttgatgtta attgcaagta cgggactaat ccccgcatta ttcggagctg ttttgcaaga
     7261 agtcgtggat acggtctcta ttttgagtgc cttgagagca aaaaaagatc cagccatttt
     7321 tcctgattca gtacccaaga caaacatagt atgatagaat cgagttgaaa aaagaacgat
     7381 cagaaagagg agagagcatt atgccattcg tacatgtcga attagtagaa ggtcgcagtc
     7441 cggaacaatt agaaaatatg atgaaagata tcacggaagc tgttcataaa aacacgcagg
     7501 caccaaaaga acacatccat gtcatcatca atgagatgaa aaaaggaaca tacggtgtaa
     7561 atggggaatg gaaaaaataa gtttagtttc ccacttgatt tttctattta ttagtggtta
     7621 aataaacaca taattgattg atgagaaagc caactgaatt tgagagtctt cgataagaga
     7681 ggaaaagcta ggctgaaact tttccaggag aaaaccaaag gatgaccact ttcgaaaccc
     7741 ttgttgaagc aagggcggtc ggaccgttac aacgcttgag gaacgcattt gcgtttgaac
     7801 attaggtgga accacgaact tttcgtccta gtatctcttt tcaagagatg ctaggatttt
     7861 tttgtttttc agcttgatga tctgatcaat caaaacattt caaggaggaa actattatga
     7921 taaaaattac ttttccagat ggtgcagtca aagaatttga gtctggaaca acaacattgg
     7981 caattgctga aagcatttca aaaagcttag caaaaaaagc tttagctggt aaagtgaacg
     8041 gaaaactcgt tgatttgact cgtccaatcg aggaagatgc aagtattgag atcatcacac
     8101 cagaccatga agatgcactt ggcttagtcc gtcattcagc agctcatttg atggcccaag
     8161 ccatgcgtcg cctctatcca aatatccact ttggcgttgg accagctatc gattcagggt
     8221 tttactacga tacagataat ggacaaaacc aagtaactgc agaagatttg ccggcaatcg
     8281 aagcagaaat gatgaaaatc gtcaaagaaa atctgccgat cgaacgccgt gttctttcaa
     8341 aacaggaagc attagaaatt tttgcaagtg atccgtacaa agtagaactg attagtgaat
     8401 tgccagaaga agaagtgatt actgcttatc aacaaggcga attcatcgat ttgtgtcgtg
     8461 gaccacatgt tccatccact ggccgtatac aagtatttaa attattatca gtggcaggtg
     8521 cttattggcg tggaaactca aacaatcaaa tgatgcaacg tgtatacggg acagcctttt
     8581 ttgacaagaa agctttgaaa gaatatatcc gtctccgtga agaagcaaaa gaaagagacc
     8641 atcgtaaact aggaaaagaa cttgacttgt tcatggtttc cccagaagta ggttctggtt
     8701 tgccattctg gcttccaaaa ggggcaacaa tccgacgcac gatcgaacgt tatatcgtag
     8761 acaaagaagt tagtctaggt taccaacatg tatatacacc aataatggga gatgttgagt
     8821 tgtacaaaac atctggacac tgggatcatt atcaagaaga catgttcccg ccaatggata
     8881 tgggagatgg cgaaatgctt gttcttcgcc caatgaactg ccctcatcac atgatggtct
     8941 acaaaaacac gatccattct tatcgtgaat taccaattcg tatcgctgaa ttaggaatga
     9001 tgcatcgcta tgaaaaatca ggtgctttgt caggtttaca acgtgtacga gaaatgacgt
     9061 tgaatgatgg tcatacattt gttcgtcctg atcaaatcaa agatgaattt aaacgtacgc
     9121 ttgaattgat ggtggcagta tatgctgact tcaatattac agattatcgt ttccgcttga
     9181 gctatcgcga tccaaacaat acagataaat attttgatga tgacgcaatg tgggaaaaag
     9241 cccaagcaat gctgaaagcc gctatggatg aattagaact agattacttt gaagcagaag
     9301 gcgaggcagc cttttacgga ccaaaactag atgttcaagt caaaacagct cttggtacag
     9361 aagaaacatt atcaactata caattagact tcttattgcc agagcgcttt gatttgacat
     9421 atgttggaga agacggggaa aatacgcatc gtccagtagt tatccaccgc ggaattgttt
     9481 ctacaatgga acgttttgtc gcttatttaa cagaagtata caaaggcgct tttccaacat
     9541 ggttggcacc tatacaagca acaatcatcc ctgtttctgt tgatgcacat ggcgattatg
     9601 cttatgaaat caaagaacgt ttgcaaatga aggggttgcg tgtagaagtt gatgatcgta
     9661 atgaaaaaat gggctataag atacgtgcct ctcaaacaca aaagatccct taccaattag
     9721 ttgttggaga caaggaattg gaagatgcaa cagtgaacgt acgtcgttat ggaagtaaag
     9781 aaacagctgt agaagatttg aatatcttta ttgatgctat ggaagcagaa gtcaaaaatt
     9841 acagcagaga aaactaatag gtaaatttcc tgtaaaaaag tagtagatag aaagcaatcc
     9901 gaacaataga taacaggtat tcctcaaaga cacagatgct caatagaatc tgtgggattg
     9961 atgggataag atattctgtt gggcggattg tttttttgtt ttttatccag tatacgaccg
    10021 tgaataaggg tctgatatgg tatacttaca tagactatca ttaaaagggt ggaggaactt
    10081 atgagcgtaa ttagtcgtat tgaaagtatg atggaagatt attcttcagc agaaaaaaag
    10141 ttggccaact acattatcgg taatattgag aaggtcccta caatgacagc taatgaatta
    10201 gcagaagctt ccgggttgag tgcgccgaca gttgtacgtt tttccaaaaa aatcggtttt
    10261 caaagtctga cggacttcaa aatcacgatt tcaacagaac tacagactgg aatcgatgaa
    10321 ggttttagtg atatagaagc aaatgagtcc ttttactcta tcaaaaacaa acttggtaac
    10381 aatgcacaag tagcgataaa agaaacagta gatattctag aagaagaaac gatccaaaaa
    10441 gtcgtagagt gtttggaatc ggcagagact gtttttttat atggggttgg cgcctcttct
    10501 ttagtagtag aagatattct gcaaaaatgg tctcgtgtcg gtaagccaat catttttgaa
    10561 aaagatatcc acgtcttgct cccgcaactt gtcagcaatg agaaaaagaa agtgctttgg
    10621 cttgtttcaa actcaggtag aagtgcagat gtagtcgcat tagccgagct ggcgaaaagc
    10681 atgaatatcg aaatcattgc gttgacgcaa tttggaaata acccgcttag caagatagcc
    10741 gatgtactcg tacaaacatc tagaccaaaa gaaatcacga atcgaagtgc ggcaactaat
    10801 tcacttttgg cccaatttgc tacgatcgat atcatttttt atttctatat ggctaaaaac
    10861 gaaaagctta gcgaaaaagt gaaaaaaaca cgagaagcaa tccaagaata ttt
//
LOCUS       DARBER010000087        10532 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARBER010000087 DARBER010000000
VERSION     DARBER010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.090"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SCK06_002018"
     CDS             complement(25..162)
                     /locus_tag="SCK06_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1152011.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="SCK06_002019"
     CDS             complement(159..1343)
                     /locus_tag="SCK06_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG1152012.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="SCK06_002020"
     CDS             complement(1599..1853)
                     /locus_tag="SCK06_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152013.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="SCK06_002021"
     CDS             complement(1878..3020)
                     /locus_tag="SCK06_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG1152014.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="SCK06_002022"
     CDS             complement(3080..4432)
                     /locus_tag="SCK06_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1152015.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="SCK06_002023"
     CDS             complement(4503..4847)
                     /locus_tag="SCK06_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1152016.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="SCK06_002024"
     CDS             complement(4857..5231)
                     /locus_tag="SCK06_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1152017.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="SCK06_002025"
     CDS             complement(5244..5558)
                     /locus_tag="SCK06_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1152018.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCK06_002026"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCK06_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="SCK06_002027"
     CDS             complement(8976..9638)
                     /locus_tag="SCK06_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152019.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="SCK06_002028"
     CDS             10255..10485
                     /locus_tag="SCK06_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152020.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DARBER010000088        10512 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARBER010000088 DARBER010000000
VERSION     DARBER010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.091"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            12..84
                     /locus_tag="SCK06_002029"
     tRNA            12..84
                     /locus_tag="SCK06_002029"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="SCK06_002030"
     tRNA            88..160
                     /locus_tag="SCK06_002030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="SCK06_002031"
     tRNA            168..249
                     /locus_tag="SCK06_002031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="SCK06_002032"
     tRNA            267..339
                     /locus_tag="SCK06_002032"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="SCK06_002033"
     tRNA            351..422
                     /locus_tag="SCK06_002033"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="SCK06_002034"
     tRNA            439..524
                     /locus_tag="SCK06_002034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="SCK06_002035"
     tRNA            535..608
                     /locus_tag="SCK06_002035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="SCK06_002036"
     tRNA            618..691
                     /locus_tag="SCK06_002036"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="SCK06_002037"
     tRNA            711..783
                     /locus_tag="SCK06_002037"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="SCK06_002038"
     tRNA            811..884
                     /locus_tag="SCK06_002038"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="SCK06_002039"
     tRNA            898..971
                     /locus_tag="SCK06_002039"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="SCK06_002040"
     tRNA            995..1084
                     /locus_tag="SCK06_002040"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="SCK06_002041"
     tRNA            1097..1170
                     /locus_tag="SCK06_002041"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="SCK06_002042"
     tRNA            1189..1264
                     /locus_tag="SCK06_002042"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="SCK06_002043"
     tRNA            1266..1336
                     /locus_tag="SCK06_002043"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="SCK06_002044"
     tRNA            1350..1423
                     /locus_tag="SCK06_002044"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="SCK06_002045"
     tRNA            1448..1535
                     /locus_tag="SCK06_002045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="SCK06_002046"
     tRNA            1557..1628
                     /locus_tag="SCK06_002046"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="SCK06_002047"
     tRNA            1651..1740
                     /locus_tag="SCK06_002047"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="SCK06_002048"
     tRNA            1753..1826
                     /locus_tag="SCK06_002048"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="SCK06_002049"
     tRNA            1845..1917
                     /locus_tag="SCK06_002049"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="SCK06_002050"
     tRNA            1921..1993
                     /locus_tag="SCK06_002050"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="SCK06_002051"
     tRNA            1998..2068
                     /locus_tag="SCK06_002051"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="SCK06_002052"
     tRNA            2081..2154
                     /locus_tag="SCK06_002052"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="SCK06_002053"
     tRNA            2166..2237
                     /locus_tag="SCK06_002053"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="SCK06_002054"
     CDS             2503..3696
                     /locus_tag="SCK06_002054"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG1152021.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="SCK06_002055"
     CDS             3826..5298
                     /locus_tag="SCK06_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1152022.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="SCK06_002056"
     CDS             5381..6169
                     /locus_tag="SCK06_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG1152023.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="SCK06_002057"
     CDS             complement(6236..6904)
                     /locus_tag="SCK06_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG1152024.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="SCK06_002058"
     CDS             7019..8731
                     /locus_tag="SCK06_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1152025.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="SCK06_002059"
     CDS             8734..10503
                     /locus_tag="SCK06_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1152026.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
BASE COUNT         3017 a         1921 c         2442 g         3132 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gt
//
LOCUS       DARBER010000089        10192 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DARBER010000089 DARBER010000000
VERSION     DARBER010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.092"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..625
                     /locus_tag="SCK06_002060"
     CDS             119..625
                     /locus_tag="SCK06_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG1152027.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="SCK06_002061"
     CDS             642..1541
                     /locus_tag="SCK06_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG1152028.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="SCK06_002062"
     CDS             1628..2512
                     /locus_tag="SCK06_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1152029.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="SCK06_002063"
     tRNA            complement(2704..2775)
                     /locus_tag="SCK06_002063"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="SCK06_002064"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="SCK06_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG1152030.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="SCK06_002065"
     CDS             4318..5409
                     /locus_tag="SCK06_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG1152031.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="SCK06_002066"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="SCK06_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1152032.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="SCK06_002067"
     CDS             6561..7751
                     /locus_tag="SCK06_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG1152033.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="SCK06_002068"
     CDS             7855..8610
                     /locus_tag="SCK06_002068"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG1152034.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="SCK06_002069"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="SCK06_002069"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG1152035.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2629 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg
    10141 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DARBER010000090        10133 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DARBER010000090 DARBER010000000
VERSION     DARBER010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.093"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="SCK06_002070"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="SCK06_002070"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEG1152036.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="SCK06_002071"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="SCK06_002071"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEG1152037.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="SCK06_002072"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="SCK06_002072"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEG1152038.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="SCK06_002073"
     CDS             complement(4028..5044)
                     /locus_tag="SCK06_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1152039.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="SCK06_002074"
     CDS             complement(5146..5343)
                     /locus_tag="SCK06_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152040.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="SCK06_002075"
     CDS             complement(5315..6634)
                     /locus_tag="SCK06_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEG1152041.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7375..8355)
                     /locus_tag="SCK06_002076"
     CDS             complement(7375..8355)
                     /locus_tag="SCK06_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG1152042.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="SCK06_002077"
     CDS             complement(8368..9246)
                     /locus_tag="SCK06_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEG1152043.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="SCK06_002078"
     CDS             complement(9262..10062)
                     /locus_tag="SCK06_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HEG1152044.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
BASE COUNT         2733 a         2351 c         1716 g         3333 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattcttttt gattatctgt caa
//
LOCUS       DARBER010000091        10073 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARBER010000091 DARBER010000000
VERSION     DARBER010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.094"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            110..889
                     /locus_tag="SCK06_002079"
     CDS             110..889
                     /locus_tag="SCK06_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152045.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            935..1879
                     /locus_tag="SCK06_002080"
     CDS             935..1879
                     /locus_tag="SCK06_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1152046.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1895..2674
                     /locus_tag="SCK06_002081"
     CDS             1895..2674
                     /locus_tag="SCK06_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG1152047.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2686..3384
                     /locus_tag="SCK06_002082"
     CDS             2686..3384
                     /locus_tag="SCK06_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG1152048.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3412..4176
                     /locus_tag="SCK06_002083"
     CDS             3412..4176
                     /locus_tag="SCK06_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG1152049.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4220..5671
                     /locus_tag="SCK06_002084"
     CDS             4220..5671
                     /locus_tag="SCK06_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1152050.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5703..6839
                     /locus_tag="SCK06_002085"
     CDS             5703..6839
                     /locus_tag="SCK06_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1152051.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6839..7945
                     /locus_tag="SCK06_002086"
     CDS             6839..7945
                     /locus_tag="SCK06_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1152052.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            7951..8973
                     /locus_tag="SCK06_002087"
     CDS             7951..8973
                     /locus_tag="SCK06_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG1152053.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            8995..10050
                     /locus_tag="SCK06_002088"
     CDS             8995..10050
                     /locus_tag="SCK06_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1152054.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         3688 a         1426 c         2080 g         2879 t
ORIGIN      
        1 ttctgtgaat atattagttt aattatgttt ttttaaataa aaagctgtca tttttatgta
       61 agtgaattat atcttaattt atctatcatc aatacttagg agtgaatcta tggaaaagcc
      121 attcgtcaag tcattgactg ctgtggcggt aggtgcaggt gctattgcga tctgtttgtt
      181 tggataccat atcaataatc agcggcaaca tcagcaacgg atcaattatg cagaatctgc
      241 catcaccaat caaaaagaca ccttgacttc tttatcaaaa gaagtagaca agctgtatag
      301 tacaaaagaa aaaatttttc taaaccctga aataacagaa gaaacaatca caagtttgtc
      361 aaataagcta tcctctgtca aactttcagc agatgattac gacatcaaag aatcagaatt
      421 gcctaaagaa gctgttgcaa tccaagatga gaagaaagca atcgtgacac agttagatag
      481 tgcagaatca aaactgaaga tccagacagc tgtcaacgat ttatttacta aaaatgtatc
      541 aaactggcag caagctgtag acgatgtcat catcaaagaa aaggtgacaa gtgcagatgt
      601 cgctcgtgta cgtgaaaata tgagcttttt taaagatagt ccttggaaaa cggttgttat
      661 gcaatatcta ggattcgctg atattcagat cgcgcaagtc acgcaactag atcagttatt
      721 cgatacgatg ttaaaggatg gtcaagtgac tgccaccgct acttacgatc aatatttgac
      781 ggtgctcagt caaatagagc agatccggaa tgaaaaaata agcgcagcat acgcaacaaa
      841 agcacaaact gttgctgagc aaatgggcta tagcaatata agctattgac agttaaaacg
      901 ttgattcaag caaggttagc aaacggagga aactatggca agacgaagaa gaaaatcaag
      961 agggaaaaaa atatttctga ttattttagc agttcttatc gtattagtat tagcaattgt
     1021 aggagtagga gcaaaattat atctggatct ctcacattct attgagaaaa cttacgaatc
     1081 agtggaacga acccaaggag aacgaacgaa accagttgat ctggacaaac aagaatcttt
     1141 ctctgttcta ttgatgggga ttgatactgg agatttaggt cgggtcgatc aaggaagatc
     1201 agatacgatg atggttgcca cagtcagtcc tgaagacaaa cagacaacga tcgtcagcat
     1261 tcctcgtgat acgtatgtcg acatcgtagg tcgtgacaca aaagacaaga tcaaccacgc
     1321 ttacgcattc ggcggagctg ccatggcgat ggataccgta caaaaatatg tagacatccc
     1381 aatcgatcac tatgtctcta tcaatatgaa agggctaaag gaactcgttg atgcagttgg
     1441 tggtatcgaa gtcaataatg acctcacttt ctcacaagat ggctatgact ttccaatcgg
     1501 taaaattaca ttgaacgggg atcaggcatt atcttattct cgtatgcgtt atgaagaccc
     1561 aaatggtgat tacggtagac aagggcgcca aagaaagatc gtagaaggcg tagcgaaaaa
     1621 agtattgagt ctaaacggtg tcagcaacta tcaatccgtt ttgaatgctc tagagtcgaa
     1681 tatgaaaaca gacatgaact ttgatgaaat gaaaaaaatt gctttagatt atcgggaggc
     1741 atttggtacg attaagcaag atcagctgca aggagaaggc ttcatgcaag ataatatctc
     1801 ctatcaaaaa gtcagtgatc aagaacttga tcgtgtgaaa aaagaattaa aagatcaaat
     1861 gaatctagag aataaatagg aaagtggaga aaaaatggaa gagacaatca gcttgaaaga
     1921 aatttttgaa acattacgca aacacttggt aataatcatc atctcaatgt ttgctggctt
     1981 ggccatctca ggaatcgtaa catttttcat cttgacacca aaatacagtt cacaagcaca
     2041 attgatcgtg acactgccac aatcagacac tgcaaatgtc aatgatgtca atacgaacct
     2101 gcaaatgatc aatacgtata aagatatgat cgtcagtgat ttagtgctca atgaagtcaa
     2161 agatcgttta gaaacagaag acaatgtgaa aatgactgct gaacaaatca aagatgccat
     2221 ctcagttaat caggcagaaa attcacaaat gttctcgatt caggcaataa gtacgaatgc
     2281 agtggctgct caacagatcg ctaacacaac ttcacaagtt ttccaagaga acgcaaaaga
     2341 tgtgatgaat gttgataaaa tctcgatcat ttcaggtgca gttgccaata cgacacctgt
     2401 ttcgccaaat aataaattga atttaatgac tggcttaatg ttaggaatga tggtaggtgt
     2461 aggtctggca ttcttattgg aattactcga tcggacagtg aaagatgaaa aatatgtgac
     2521 agatacatta ggattcccaa tcttaggaac agttccagaa atgtcagcga aagaattgaa
     2581 tgctggtatc cgtaaaaaag gaccagctca gacacaacca aaagctacaa acgataaaac
     2641 agcgccatca cgcagaagcc gctcaagagt ctaggaggaa agaaaatggc acgaacacag
     2701 aaacaaaaag cagcccaaac tcatgcagtc agtttggtca cactcaccaa tccctcttca
     2761 ccaatcgctg aacaatatcg tacgattcgg acgaatatcc agtttgcctc cgcagctggt
     2821 cagcaaatca aaacgattgt tgtgacttca tcaggacctg gagaagggaa atcgaccact
     2881 gcagcaaaca ttgccgttgt ttttgccaaa tctggtcaac gggtgctgct agtcgatgca
     2941 gacttacgta agcccgtcat ctataaaacg ttcaagttga acaacgcaag cggattaagt
     3001 actgttctta gttcatcagg aagtgtagca gatgagatcc aacgaacacc agtcgacaat
     3061 ttgtcgattc tgccaagtgg acctaaacca ccaaatccat cagaactact aagttcgcca
     3121 agaatggatc agattttagc agaagcaaga caactatttg atgtagtgat cttcgacatg
     3181 ccgccagtcg ttgccgtaac agatgcacag atcatgtctt caaaaacgga tggcacctta
     3241 ttggtcgtac gggaaaatac gtcacgaaaa gaatcattga ctaaagcaaa agaactgtta
     3301 gatatggtac aagcacgagt cttaggtgtc gtatacaacg gagcagaaca cagcaaagat
     3361 gctggttatt actacggaaa ttaaggaata agaagagagg agacaagaga aatgatcgat
     3421 ctacattgcc atatcctacc aggaatcgat gacggggcag aaaacttaga agcaagtatt
     3481 gcaatggctg aaaaagcgat tcagcaaggc attacgcata ttttatgcac accacaccac
     3541 aataatggaa aatacagcaa tgaaaaatca caagtcattt ctctagtcgc ttcgttacaa
     3601 gccgaattag agaaaagaca gttgcctttg acgttattgg aaggacaaga agtacgtatt
     3661 accggaacgc tcatcgaaga catccaccga gatgaaattt tatttacaga tttagatgat
     3721 acatatcttc tgatcgaatt tccgacactg gaagtgccgt tatatgcaga acggcttttt
     3781 tttgagttgt tagaactagg aaagatacca gtgatcgtcc atccagaacg caacgcgcat
     3841 tttcgagaag acccaaatca tttgcttccg ttcctagata tgggatgttt ggcccaactt
     3901 actgcgccaa gttatgttgg tgtatttggc aaagatatcc agaaaacagc caaaacaatg
     3961 gtcaaacata atctcgtgca gatggctgct tctgatgcac acggggtctc taagcgcacc
     4021 ttttacttaa aagagtgcta tgagcagatt gccaaggagt ttggtaagga aaaagtagta
     4081 cagatgaaac aagtcgcaaa ggatctgatc aacggggatg agatcaatta tccaatctat
     4141 gaagaggtaa aaaagaagaa gtttggttta ttttagttct ttattcgtag ataaaaagga
     4201 aatgaggaga gagtgagaga tgtatagaaa aaactcaagt ggttggtcta aacatattga
     4261 ttttatttta ttagacttat tttgtgtcca gcttgctttt tatatatcat atgtaatgcg
     4321 acagggtgac tggaatccgt atgtgattcc tatttatcgc cacatggcaa tttttataga
     4381 acttgagaat cttacgatta tatttttatt tgaggcatat aaaaatgttt taaaaagagg
     4441 atattataaa gaggctgtag cttctataaa gcaatctttt atgctgttac ttgtgtgcag
     4501 tttatacctt attacaatgc aggatggaaa tgattattcc agagtttctt tatttataat
     4561 gggggtggtg tatggattat taacatatat aattcgtata ctttggaaag ggttgttgca
     4621 tcaccgcatg aaaaacggtg aagatgtatc gttagttatt gtaacatctc aaaagattgc
     4681 aaaaaatgta gttcgtaatc ttaaagagaa gaattatggc atgtataaac ttagtggtgt
     4741 gattgtagta gacagagata tgtatggaac taccatcgga ggtgtcccag tagttgcaaa
     4801 tgctgataat gcagcggaat atcttttaca gaattgggta gatgaagtat tcatcaatgt
     4861 tgacgaaagt gttccatatc ccaaagaatt aatcgccaga tgttcggaaa tgggacttgc
     4921 tgtacatatg aatcttacaa aagtgtctga tagtacacgt ggaaaacaat ttattggccg
     4981 agttggtgat tatactgtta ttacatctag tattaatgtt atgacgatga ggcaagcatt
     5041 tataaagaga acgattgaca ttcttgctgg aattattgga tgtattgcaa caggtgttat
     5101 ttttatcttc gtagcaccag ttatttacat caaatcacca gggccgattt tctttgcaca
     5161 agaacgtatt ggtcaaaatg gtaagacatt taagatgtat aagttccgta gtatgtatat
     5221 ggatgcagaa gaacgcaaag cagagttgat gaaagaaaat aagatgagta ataatctcat
     5281 gtttaagatg gattttgacc caagaatcat tggcaataaa atacttcctg atggaacaaa
     5341 gaagacagga attggacaat ttattcgatc tacatcattg gatgagtttc cacaatttta
     5401 taatgtccta atgggaaaaa tgagcctggt ggggtttaga ccttgcttga agtcagaata
     5461 tgagaagtat gattttcatc atcgtgcgag aattgcgatg aaacctggga ttactggaat
     5521 gtggcaggtg agtggtagaa gtgatatcac agacttcgaa gaggttgtaa aacttgatgc
     5581 agaatatatc aggaattgga gtatgggatt agattttaga attttgttta ggacagtcaa
     5641 aactgtgctt catagaaacg gttccatgta agataaagat actatagaag gagtaaaacg
     5701 tgatggcaag aaaaaaatta aaaatctgta tgctaggtca taaaagaatt ccatcaagag
     5761 aaggtggaat tgagatagtg gtagaagaat tggcaacgcg aatggttgca cttgggcatg
     5821 aagtgacttg ctataatcga agtggtcatc atgttagcgg aaaggaattt gaccagcata
     5881 aaaataaaga atataaaggt gtaaaattaa agactatttt tacattaaat ataaaaggaa
     5941 ttgcagcaat gagttcttca gtatttggtg gaattagagc agcattcgga aaatatgatg
     6001 ttgttcattt tcatgcagaa ggaccatgtg ctatgttgtg gttgccaaaa ctttttggga
     6061 aaagatgtgt tgcaacaatt catggtctag atcatcaaag agaaaaatgg aacaagcttg
     6121 ctagtacata catcatgtta ggggaaaagt gtgctgtaaa atttgcagat gaaataattg
     6181 tacttagtga aagcgtccaa aattattttg aagatatata tggaagaaaa acaaggttta
     6241 taccaaatgg cgttaaaaaa attgaaataa aatcagcagg attgattaca gaaaagtatg
     6301 gattaacaaa agattcttat attttgtttt tggggcgatt ggttcccgaa aaaggaattc
     6361 ggtatctaat tgaggcattt aaagatgttc agacagataa aaaacttata atagccggag
     6421 gaagctctga tacagatgaa tttgcaaatg aactaaagga attagcaaaa ggtgatgagc
     6481 gaattatatt tactggattt gtacaaggac aggaattaga agaattatat agtaatgcgt
     6541 atatctatac acttccaagt gatttagaag gaatgccatt aagtttgtta gaagcaatga
     6601 gttatgggaa ctgttgtata gtttcaaata tctcagagtg tacggaagta gtagaagaca
     6661 aggctatgat ttttaagaaa tcggatgtaa gtgatttaaa gataagatta gaagaggcat
     6721 gcaatcaatc ggaaatggtt aaagttttga agaatcaagc tactgagttt atttgtagta
     6781 aatataattg ggataaaatc gtacaagaaa cattaaattt gtatagggga aatatataat
     6841 gattcgaata ttacagtgtg tgaatgatat gcatcgtgca gggttggaga cgatgctaat
     6901 gaactattat aggaatatag atcgaactaa aattcagttt gattttttga cacatcgtcc
     6961 atataaaagt gattatgatg aggaaataat aagtcttgga ggaaaggtct attatgcacc
     7021 gagactttat ccacaaaatt atcctaaata ttttaaatgg atggaacaat tttttaagga
     7081 tcatccagaa tatgagattg ttcattctca tattgatgcg atgagttatt tacctttaaa
     7141 agctgcaaaa aaagctggga ttccgatacg gatagcacat agccataata catcgataga
     7201 tagagatttt aaatatcttt taaagcaata ttatcgatgt aagttaacgt ctgttttgac
     7261 acatgaattt tcatgtggaa aagtagcagg tgattttttg tttcgaaatg ataaattcac
     7321 tttgattcca aatgcagtgg atgctaaaaa attttattat gatgaaagta ttagaaatga
     7381 tgtacgaatg aaattaggaa tttcaagtca gacgtttgta atgggacata ttggaagaat
     7441 ttcatattct aaaaatcatc gttttttgat tgaaattttt aaagaatttc acgctattaa
     7501 gaataattcc gtattattaa ttattggaac tggtgatatg gaagaagaaa taaaaaatta
     7561 tgcaaagaaa agtggcatag atgatgatat taaattttta ggaaatagaa atgatataga
     7621 aaaattttat caagcatttg acgtattaat gttgccatca ttatttgaag gggttccgtt
     7681 agtgggtatt gaagctcaat ttgcagattt accgtgtttc ttttcggaga aagttcctac
     7741 agaagttgct ttcagtgata agaccaattt cataagttta aatcaatcgg caaaagaatg
     7801 ggcgagagag attgcaaatg tagatatctc tcatcgagat tcagaacgtt cgattttaat
     7861 aaaggcagat tataatattc aaacagcata caaaatctta gaatcaaaat attatgagtt
     7921 atttgaatta atacaaagag gttaaagaat atgacacaga aacattcaag tataaaaaga
     7981 attatacatc gcatatccga agaattgaat gcaacaagac gaattgcaaa gataacatct
     8041 tggaaggaag catttgccac gttacaagct aaaattgata ttcaagtcat gaatcgtaat
     8101 ggatataatg aaagtaaaaa acagaaggaa catttattaa aaaaacatga ggtaatgctg
     8161 aagtattatg aaaagacttt ttcagatttt ttgaaaacat atgattatag tcaaaatcaa
     8221 ataattaatc aatcaaaatc accatattct aactgcattt gggtttgttg gtggcaggga
     8281 ttggatcaat cgccggaaat tgtaaaagct tgtgtaaaat caataaaacg aaatgcaggg
     8341 aaacatactg ttataatttt aacagaagat aattatagac aatatgttga tataccaaaa
     8401 tgggtggaag aaaagaaaaa caaaggaatc attacgagaa caaattattc tgatttatta
     8461 agattatctt tgctagcaaa acatggaggt ttgtggttag acgccacatt ttattgtaca
     8521 aaaccaatac cagatgagta ttttgatatc gatatttggt ctattaggag gcctgattat
     8581 gctcacgcat ccgtagcatc aggatatttt gcgggttatt cattagcatg taatcaagag
     8641 tctagatgga tgtttgctat aattagagac ttttttttaa attattggaa aaataatgat
     8701 atgatggtgg attatttgat gatagattat atgattgttt tagcacagag aataaatcat
     8761 gaaatagcac agacatttga taaaatacct agtaacaatc cattatgtga cgaattgatt
     8821 aaaaatttaa ataatatata tgatgaaaaa gtatggaact tattaaagaa agatacttta
     8881 ctgtttaagt tgacatggaa acaatctttt aaaaagcaaa aagatgataa agttactttt
     8941 tatgggatgt tggtagaaaa caaattatta taaatattag aggtataaaa agttatgaaa
     9001 cacaaggtaa gtgtgatagt accaatttac aatgttgaaa aatatttaag aaaatgtgtg
     9061 gatagtttat tgagtcagac attaaaagag atagaaatcg tattagttga tgatggttcg
     9121 ccagatgctt cgggggaaat agcagatgag tatcaaaaaa aatattctaa tgtaaaaaca
     9181 atacatagag aaaatggggg gttaggaccg gcaagaaata caggaattga gaatgcgaca
     9241 ggagaatatg tggcttttct tgattcagat gattgggtag aaagtgatat gtatgaaaaa
     9301 ttatacttag ttgcgtccaa agaaaatgct gatatagtag ttagtggaca ttgtgatttt
     9361 gcgaatgaca aagctatggt gaaaaagcaa catcctttga gtgggaatgt atatgatacg
     9421 cctaatagta ttaaaaatgt acgaaaaaat ttatttggac atagtccaaa agatcaagaa
     9481 gtagaggcct tcccgatgtc agtatgtatg tctttgtata ataaaaatct gctagataaa
     9541 tatagactaa gatttgaaaa aattttatct gaagatacaa tttttaactt gaatgcatat
     9601 gatgtcgcaa ataaaattgc ttttacagaa tatacagatt attgttatag aaaagaagaa
     9661 caagagtcta ttacaaaaag tttatctcca aataagcaga cggaatttaa agaattttta
     9721 ataactctaa aagctatagc atcgaaagaa gatttagaat gtcaaatcag agcaaaacgt
     9781 atggcaattg attattgtag gctttatgtg ggcttagttg atgatgcaaa tttgggaatt
     9841 aaagagaaaa gaaagtatat aaaaaaattt attgaagatg agggattaat tcaatgttgg
     9901 aagggatatc ctacaaatac gctaccattt caacaaaaat tttttcataa aatgcttgaa
     9961 tgtaagtgtt atacaatatt gttgttgtta agtagaatta gaaagataat taaaaatatg
    10021 accagaaaaa aattgtttat aatattataa actaaaagta tatcaaataa tag
//
LOCUS       DARBER010000092        10008 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARBER010000092 DARBER010000000
VERSION     DARBER010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.095"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(472..696)
                     /locus_tag="SCK06_002089"
     CDS             complement(472..696)
                     /locus_tag="SCK06_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152055.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            887..3664
                     /locus_tag="SCK06_002090"
     CDS             887..3664
                     /locus_tag="SCK06_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1152056.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3745..4164
                     /locus_tag="SCK06_002091"
     CDS             3745..4164
                     /locus_tag="SCK06_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG1152057.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4176..4646
                     /locus_tag="SCK06_002092"
     CDS             4176..4646
                     /locus_tag="SCK06_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1152058.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4663..5442
                     /locus_tag="SCK06_002093"
     CDS             4663..5442
                     /locus_tag="SCK06_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1152059.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5429..6250
                     /locus_tag="SCK06_002094"
     CDS             5429..6250
                     /locus_tag="SCK06_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1152060.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6267..7316
                     /locus_tag="SCK06_002095"
     CDS             6267..7316
                     /locus_tag="SCK06_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1152061.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7329..8333
                     /locus_tag="SCK06_002096"
     CDS             7329..8333
                     /locus_tag="SCK06_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1152062.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8966..9985
                     /locus_tag="SCK06_002097"
     CDS             8966..9985
                     /locus_tag="SCK06_002097"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1152063.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3360 a         1649 c         2092 g         2907 t
ORIGIN      
        1 aatctttttt gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt
       61 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
      121 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
      181 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
      241 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
      301 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
      361 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
      421 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
      481 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
      541 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
      601 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
      661 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
      721 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
      781 aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta
      841 aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt
      901 gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat
      961 gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt
     1021 cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc
     1081 agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt
     1141 ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt
     1201 attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac
     1261 agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg
     1321 taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga
     1381 ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac
     1441 tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg
     1501 ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc
     1561 agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga
     1621 acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac
     1681 tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac tacctgcact
     1741 taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca
     1801 aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca
     1861 gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc
     1921 atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga
     1981 actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga
     2041 ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag
     2101 ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg
     2161 aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca
     2221 agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat
     2281 ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct
     2341 ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa
     2401 agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat
     2461 tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc ggattttact
     2521 gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat
     2581 tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca
     2641 agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc
     2701 agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga
     2761 catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt
     2821 gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa
     2881 tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact
     2941 gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg
     3001 tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa
     3061 aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc
     3121 catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta
     3181 tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga
     3241 cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc
     3301 attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat
     3361 cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa
     3421 attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat
     3481 cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat
     3541 taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat
     3601 tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt
     3661 ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg
     3721 taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa
     3781 tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact
     3841 gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt
     3901 atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac
     3961 aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg
     4021 ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc
     4081 cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag
     4141 aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc
     4201 atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta
     4261 ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag
     4321 ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga
     4381 aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat
     4441 ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa
     4501 aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct
     4561 tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa
     4621 aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc
     4681 ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt
     4741 tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg
     4801 atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg
     4861 attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga
     4921 gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg
     4981 aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga caattatgca
     5041 gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc
     5101 ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat
     5161 ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt
     5221 ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat
     5281 tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt
     5341 tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt
     5401 caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat
     5461 aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa
     5521 ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt
     5581 atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac
     5641 gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa
     5701 ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc
     5761 tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt
     5821 gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt
     5881 caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac
     5941 gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc
     6001 tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc
     6061 tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt
     6121 tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt
     6181 gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg
     6241 gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa
     6301 aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa
     6361 aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga
     6421 agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt
     6481 tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga
     6541 aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg
     6601 ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag
     6661 gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt
     6721 tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac
     6781 aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa
     6841 cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca
     6901 ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa
     6961 tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg
     7021 cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc
     7081 aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa
     7141 aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat
     7201 tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg
     7261 acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg
     7321 tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag
     7381 ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg
     7441 tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga
     7501 atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa
     7561 acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag
     7621 aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg
     7681 ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg
     7741 gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat
     7801 tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg
     7861 aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata
     7921 gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt
     7981 gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc
     8041 acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg
     8101 aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata
     8161 cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg
     8221 ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt
     8281 tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat taggatagac
     8341 tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta
     8401 aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta
     8461 taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt
     8521 agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag
     8581 cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac
     8641 aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt
     8701 taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca
     8761 ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt
     8821 agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac
     8881 acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat
     8941 caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc
     9001 ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg
     9061 aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc
     9121 tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa
     9181 cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact
     9241 tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga
     9301 tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc
     9361 aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac
     9421 aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa
     9481 tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact
     9541 gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc
     9601 aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg
     9661 tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac
     9721 aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga
     9781 agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt
     9841 agagctagct caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt
     9901 gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat
     9961 ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DARBER010000093         9990 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARBER010000093 DARBER010000000
VERSION     DARBER010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.096"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(34..2154)
                     /locus_tag="SCK06_002098"
     CDS             complement(34..2154)
                     /locus_tag="SCK06_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1152064.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="SCK06_002099"
     CDS             2384..3562
                     /locus_tag="SCK06_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG1152065.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="SCK06_002100"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="SCK06_002100"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG1152066.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="SCK06_002101"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="SCK06_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG1152067.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="SCK06_002102"
     CDS             complement(4915..6168)
                     /locus_tag="SCK06_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1152068.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6286..7635
                     /gene="gdhA"
                     /locus_tag="SCK06_002103"
     CDS             6286..7635
                     /gene="gdhA"
                     /locus_tag="SCK06_002103"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG1152069.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7747..9093
                     /locus_tag="SCK06_002104"
     CDS             7747..9093
                     /locus_tag="SCK06_002104"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG1152070.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9401..9787)
                     /locus_tag="SCK06_002105"
     CDS             complement(9401..9787)
                     /locus_tag="SCK06_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1152071.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9836..>9990)
                     /locus_tag="SCK06_002106"
     CDS             complement(9836..>9990)
                     /locus_tag="SCK06_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1152072.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT         3185 a         1815 c         2039 g         2951 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttgtcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactag tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctttagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcatgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 aatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cgtaggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gtttgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttgtctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gctggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa aatgttttga gccaaaagag
     6241 aaaatgagct aaaatgaggg aagataagca aaggagcgtg gaacgatgac aaacgcaaca
     6301 gaatatgttc aagcaattca agaaaagcta catcaaaagg atcaagggca agaagaattt
     6361 ttacaagcaa tcgatgaatt catgcccacg gttatgtctt ttttggacac tcatcccgaa
     6421 tatatcgaaa agaatatctt agaactttta acggaaccag aaagagttat tcaatttcga
     6481 gtgccttggc aagatgatga aggaaactgg cgagtaaata gagggtaccg tatccaatac
     6541 aactcagcta tcggccctta taaaggtgga ttgcgcttcc atcctagtgt caatctaagt
     6601 atcttgaaat ttttggcttt tgaacagatt ttcaagaata gtttaaccgg tctgccaatc
     6661 ggtggaggaa aaggcggaag tgacttcgat cctaaaggaa aatctgataa tgagatcatg
     6721 cgtttttgtc aaagtttcat gctggaattg tcaaaacata tcggcccatc gattgatgtt
     6781 cctgcgggag atatcggtgt aggagctaga gaaatcggct atttgttcgg cgaatataaa
     6841 cgattgaaaa aatatgatac tggtgtgttg acaggtaaac ctttggattt ttggggaagt
     6901 aagatacgta cggaagctac tggttatgga ttagtgtatt atgtcaaaca tctattgaac
     6961 gaagaaaaag attcatttaa tcaaaaaaca gtttttgttt ccggaagcgg gaatgtagcc
     7021 atttatgcaa tcgaaaaagt gcaagagcta ggcgggaaag ttgttacatg ttcagattct
     7081 agcgggtaca tctatgatcc agaaggaata gatgtgaaac tattgaaaga agtcaaagaa
     7141 atcaatagac aacgtttgac ggaatacgca aaccaacgtc cttctgctat ttatcatgct
     7201 ggagaatctg tttggacatt gaaagaaaaa gcggatatcg ctttgccatg tgctactcaa
     7261 aatgaaatag acgaaagctt ggccgaaatc ttagtagaaa atgggatcaa aatcgtagca
     7321 gaaggcgcta atatgccatg tacagtaaaa gctgtttctg ttcttcatga agcaggcgtg
     7381 tggtattgtc caggaaaagc tgctaatgct ggaggagtag cagtatctgc tttagaaatg
     7441 agtcagaacg tccagcgatt gacttgggaa agagagcaag tggatgagca gctggatcaa
     7501 atcatggaaa cgatttacca tacgtgtcgc caaacagcag ccgagtacgg cgacgaaaaa
     7561 aatctgcttc ttggagcaaa tgttgctgga tttgaaaaag ttgccaaagc aatggcagca
     7621 gaaggtttag tttgaaaaat gaaactttct tggaaatatt gtcagcattt tgtttatttg
     7681 cttgaaaaat aataaaaaaa aacgtaaagt attacctgta gagtaaacct ataaggagga
     7741 gaacaaatgg cacacattca ttttgactat tcaaaagtag caccgtttgt caacgaacaa
     7801 gagttagggt acatgcaaag ccaagtaaca gcagcccaca acgaattacg ggaaggtact
     7861 ggcgcaggaa gcgacttccg cggctggatc gatttaccaa cgaactatga caaagaagaa
     7921 tttgcacgta tcaaagaagc agctaaaaaa atccaatctg attctgaagt attagttgtt
     7981 atcggtatcg gtggttctta tttaggcgct cgtgcagcga tcgacttctt gaatcacaca
     8041 ttcttcaatt tattagatag caacacaaga aaagcaccac aaatcttctt tgccggaaat
     8101 tcaatcagct caacatatat tgctgacttg atcgaagtaa tcggtgatcg cgacttttca
     8161 gtaaacgtta tttcaaaatc tggaacaact actgaaccag cgattgcgtt ccgtgtattc
     8221 aaagagcttc ttgttaagaa atatggacaa gaagaagcaa acaaacgtat ctacgctaca
     8281 acagataaag caaaaggtgc agttaaagta gaagcagatg cagaaggttg ggaaactttc
     8341 gtgattcctg atgacatcgg cggacgtttc tctgtattaa ctgctgttgg tttgttacca
     8401 atcgctgtta gtggtgcaga tatcgatgca ttgatgcaag gtgccgctga tgcaagcaaa
     8461 gcatactcaa gcgacaaact agaagaaaac gaagcttacc aatacgcagc aatgcgcaac
     8521 attctttacc gtaaaggcaa agtaactgag ttgttgatca actatgaacc aggaatgcaa
     8581 tacttctcag aatggtggaa acaattgtat ggtgaatctg aaggaaaaga ccaaaaaggc
     8641 atctatcctt caagcgcaaa cttctcaact gatttgcact ctttaggaca attcatccaa
     8701 gaaggacgtc gcaatatttt tgaaacagtt gtgaaggttg aaaaacctcg taaatcaatc
     8761 acaattcctg aacaagcaga agatttagac ggtctaggct acttgcaagg aaaagaagtg
     8821 gacttcgtaa atacaaaagc atttgaagga acattgcttg ctcatacaga cggtgacgtg
     8881 cctaacttgc tagtcaaaat tccagaaatg gatgcttata cattgggtta tacaatgtac
     8941 ttctttgaaa ttgctgtagg tatttcaggt tacttgaatg gaatcaaccc attcgaccaa
     9001 ccaggtgtag aagcttacaa gaaaaacatg ttcgcacttc taggcaaacc aggctttgaa
     9061 gatcttgcta aagaattgaa cgaacgcctg taagataagg gtttttcgct agatttaaat
     9121 aatcagtgat agaaacatgt tcgctttaaa atgaaaactg gtacccattt aggtactcaa
     9181 acatactgca tcgtttattc agaataaggg gctgtgacaa gacgtttgtc acagtccctt
     9241 gtttgtatag aaatcgattg attgccttgc tctttagttt gttttaggac tttaagaaca
     9301 gtgaggctga cttcgaaggt taattctgtt tttttgtttt ctagaatcag taagcaaaaa
     9361 agcactcact tcgagtgctt ttttgcgata tattcttagc ttaagatagg cctaattttt
     9421 cttttacaga tgagggtacc ttatcgctgg taattacttt tttctctatg atccgctttt
     9481 cgcttgcttt caactgcaag atcgttcctt cggggtaagc agaaccattg gcagagtcac
     9541 cagaagtaat gacatcgaaa tctaactgtc ttttttcacc gttttcagca taagcatcta
     9601 cgttgtattt aaaacctctt ccaattactt ccccattatc tgctttgata tcagtttctg
     9661 caggcattgg agctttgact accgcataat aatctttacc tacataagta gactgatagt
     9721 agtcccaacc tttccatcct ataaaagcaa tcaatatgat aacgatcaac cctaaaattt
     9781 ttttcatgaa agatctcctt ttttattttt attatacccg tggtgctagt taattttaat
     9841 atcctaaaga atatctcaaa gttccttcaa aacgtcgttg tgctcgactt aatagaacac
     9901 tgtaagccag taatatgctt tcaaatctac tctctagccc tttaacagaa cgtgagttga
     9961 aattttgaca acctaatttt tctaaggaag
//
LOCUS       DARBER010000094         9942 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARBER010000094 DARBER010000000
VERSION     DARBER010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.098"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            28..930
                     /gene="gnd"
                     /locus_tag="SCK06_002107"
     CDS             28..930
                     /gene="gnd"
                     /locus_tag="SCK06_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG1152073.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            932..1933
                     /locus_tag="SCK06_002108"
     CDS             932..1933
                     /locus_tag="SCK06_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG1152074.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1930..2214
                     /locus_tag="SCK06_002109"
     CDS             1930..2214
                     /locus_tag="SCK06_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG1152075.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2247..3044
                     /locus_tag="SCK06_002110"
     CDS             2247..3044
                     /locus_tag="SCK06_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1152076.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3058..3891
                     /locus_tag="SCK06_002111"
     CDS             3058..3891
                     /locus_tag="SCK06_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1152077.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3909..4766
                     /locus_tag="SCK06_002112"
     CDS             3909..4766
                     /locus_tag="SCK06_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1152078.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4788..5297
                     /locus_tag="SCK06_002113"
     CDS             4788..5297
                     /locus_tag="SCK06_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1152079.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5334..5735
                     /locus_tag="SCK06_002114"
     CDS             5334..5735
                     /locus_tag="SCK06_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152080.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5847..6200
                     /locus_tag="SCK06_002115"
     CDS             5847..6200
                     /locus_tag="SCK06_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152081.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6234..6677
                     /locus_tag="SCK06_002116"
     CDS             6234..6677
                     /locus_tag="SCK06_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1152082.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6694..7779
                     /gene="uxuA"
                     /locus_tag="SCK06_002117"
     CDS             6694..7779
                     /gene="uxuA"
                     /locus_tag="SCK06_002117"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1152083.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7772..8485
                     /locus_tag="SCK06_002118"
     CDS             7772..8485
                     /locus_tag="SCK06_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1152084.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8692..8841)
                     /locus_tag="SCK06_002119"
     CDS             complement(8692..8841)
                     /locus_tag="SCK06_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152085.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9237..9437
                     /locus_tag="SCK06_002120"
     CDS             9237..9437
                     /locus_tag="SCK06_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1152086.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3355 a         1546 c         2200 g         2841 t
ORIGIN      
        1 gagggtataa gaaatggagg cgctatcatg gaaattggtg taattggttt gggaaaaatg
       61 ggattgaatt tagctttaaa cttaaaagag caccatcaca aagtacaagg actcgacatt
      121 tcaagtacag ctgttagtca ggcaagagaa aatgacattc ctgcttatca agagatctct
      181 gaatttctag ctacttttga gcagaaaaaa atcatctggt taatgcttcc tgctggagag
      241 ataaccgaaa acatgttaag aaaacttttt ccaaaactcg gaccaggaga cattattatt
      301 gaaggcggaa actcttatta taaagattca atccgtcgag cagaagagtt tcagaaaaat
      361 gaaatcggtt acttggattg tgggacttct ggaggaatcg aaggagcacg acatcaagcc
      421 tgcttaatga ttggaggaga taaagaaact tttctatccg tggaacaatt attcaaagat
      481 gtagcaattg aaagaggata tctgtatgct ggtgcatccg gaagcggaca cttcttgaaa
      541 atgatccata atggaatcga atatggaatg atgcaagcaa tcggtgaagg attccaactt
      601 gtacaaaaaa gtgagtatgc ttttgacttg gaacaagtcg cacgtgtttg gaatcatggt
      661 tcggtgattc gcggctggct gatggaaatc atggaagatc agttaaacga acatcccaat
      721 ttaagtaatt atcggggaat cgtttctgca tcaggtgaag caaaatggac ggttgaaaca
      781 gcgctagaaa tggctgttcc agttccagta attgcaacta gtttgttcat gaggaatctt
      841 tcacaggaag aagatagttt ttcagcaaaa gtagtttcag cactaagaaa tgggtttggc
      901 ggacatgaaa tagttgaaaa gggtgagtaa aatgccagat tatcatatag caatcgttaa
      961 ttccagcagt ttcggaagag tctttccaca ccacttggaa agattaaagt cagttggaga
     1021 cgttcatttc tttactgttg atcaagagat tccaggaaaa gaactagcgg agttacttaa
     1081 aggattcaat atcattattg caagtgttac gccatttttt cgacgagatt tttttgaaaa
     1141 caaagatgaa ctgctattga tttcccgaca tggtattggt tacaacaata ttgatttaaa
     1201 agcagcaaaa gaacaccaga caatcgtatc tatcatacct gctttagttg aacgaaatgc
     1261 tgtagcagaa aataatataa ctaatttgtt gtccatgctt cgacgcacga cacaagcaca
     1321 agcaagagtc aaggacgatc gatgggaaga tcgggcggag ttcgtcggta ggacgctgtt
     1381 taacaaaaca gttggtgtga ttggtgcagg aaataccgga agcggagtaa tcgaaatttt
     1441 gcgtaacggt tttcgctgtg atgttctagc ttatgatcct tacaaatcag atttatacat
     1501 cgaaagcttt ggtgcaaaaa aagtagaact tcaagaatta ctggaacaat cagatatcat
     1561 ttgtttatgt gcgaatttga cagaagagaa ctatcacatg atttctgata aagaagtcga
     1621 tcagatgaaa gaaaatgttt atctatctaa tagtgcaaga ggcgctctag ttgatgaaga
     1681 agcgatgatt aatggtctga aaagcggaaa gatcgcagga tatgccacag atgttttaga
     1741 agaagaaccg ggacgtcaag atcatccgta tttagcgttt gaacaagtag tgattacgcc
     1801 acatatatcg gcctacacaa gggaatgttt agaagcaatg ggtgaaaaat gtgtcagaga
     1861 tgtcgaacag atcagtcagc accttcttcc tgaacgttct gtccagacaa agagcaacta
     1921 tataaaagga tgatgaagaa aatggtgaac ggtttaaaag tgaaaacagg ccctcaattt
     1981 tatttatatg aagaaggtgg tatttcaaaa gtttctgatc tcttgaaatc atacggcgcg
     2041 aaaagagtac tggtcaccca cggaacggta tcatgggaaa aggcgctacc taaattagta
     2101 tttttgaatg acgaaacgat tcaatttttt tatcatcgtt attctggaga atgtagttat
     2161 gctgaagcca gacgaatagc tacaatcatc aaaaaaatga aatcgatttc gtagttggcg
     2221 ttggcggagg aaaactgacc gatctagtgg gttatagtgc caatcttgca agaatcgatt
     2281 tcggagtcat ccctacttta gctagtaatt gtgctccatg gacacctctt gctgtcatgt
     2341 ataaggagaa tggacaagca gaagggaaaa cagaacactt ttacagacaa gctgcttttt
     2401 ttataacaga tccccaatta gtgattgatt cccctgttcc atatttcatt gccgggttag
     2461 cagatacact agcaaaatgg tatgaatctg agctcatctt agaacaagat tttttgcaag
     2521 atgaatcatt tttgaagcta gcacaagata ctgcgaagat ctgcaaagaa gagattttga
     2581 atcattctgc taaagcaatc caagatatgg cacaaagaaa actgacatcg gagttcaaac
     2641 agctttcaga gattatcttt gctgttgctg gtttagttgg aggattagga gataaatacg
     2701 caagaaatgt ggcagcacat gctatgcatg acgctatcag taaatatatt ccggaaagcc
     2761 atcgttttct acatggagaa aaagtagcgt atgggatttt ctatcaactg gcactagaag
     2821 gtcgctggga aaaaatagac gagttatatc ctttgtatcg ggaattagaa ctgccggttt
     2881 ctctagaaca aatgggatta tttccgaaag ataaacagat aatagataaa atggctgaat
     2941 ttattgattc gaaagagaaa gtgcatttga ttcctataga ggtaacacag aaatcactga
     3001 tacagtcaat gatggaactg gaaggttata tcagaaagat ctgaaggagg aggacacatg
     3061 gaaacattaa gtgtagtaca aagtttgttg attgctttat gggtagccgc ggttatgtcc
     3121 cgctggttag gtggaggagc cacgttgacc ttacgttttt ctcctttgat gactggatta
     3181 gtcgtcgggt tgatcatggg ggaagttgca caagcgatga ttgttacagc cgctttgcaa
     3241 atgatttata tgggggtttt ttctccagga ggatcaatgc cagctgaacc atctattgca
     3301 gcagctatcg cagtaccagt cgcattatta ggggatctgt cgccagaagc agcgattgcc
     3361 gttgcggtgc cagtcggatt gctcggaagt tacttgtatc agtttcgttt tttcatcaat
     3421 acttttttag gtaaatatac agacaaagct gtagaagatt tgaatacaaa agctatggcc
     3481 cgatcaatta ttctttatcc aacggttgcc tcatttttat tattcgttcc gcttgttttc
     3541 attgctttat atttaggggc gccattgatc gctgatgtaa tcaaagcttt agagggaaca
     3601 gtcgttatcc acattcttga agtagtaggt ggagggttag cggcaatcgg tatcgcgaca
     3661 actgtatacg ttatcggtcg aaaagattat ctggtctttt tcttccttgc ttattttatg
     3721 agtgttgttt tgaaatcttt agatattacg atggttactt atgccatttt tgggattatt
     3781 atcgctttga tctttgtcca gtcgcaaaaa gggaaacaag tacagcctgc cacaagcaat
     3841 aacaattcag catcttttga tgacgaagat gactatgatg acggatttta atttaggagg
     3901 aatgggaaat ggctgaaata atggataatc aaacaggtgc tcctgaagaa atcacgaaaa
     3961 aagatgtaac aaaagcctat ctgagatggc attttgcaaa cgaaattcct cactcttttg
     4021 aacgttactt ggcgccatca ctactatacg gaatgatgcc aatcttgaaa aaactttata
     4081 aagatgaaga ctctttaaaa gcagcatata aacggcagtt actgttcttc aacactcaat
     4141 taagctgggg tggcggtgtg attactggcc taatgtcttc tatggaacag caaagagcag
     4201 aagaagagta tgaagaaaaa gagatcttga tgcaggatga cttgatgtat aacacgaaag
     4261 cgggattgat gggggcacta gcaggaatcg gagatgcaat cgattcagga accgtccaat
     4321 atatttttat cgcaatcgcg gtaccttggg cacaacaggg aagtccttta ggcgcacttt
     4381 ttccttttat ctgctttggg ttgtatcagg ttttactagg ggtatttttc gcaagacaag
     4441 ctttcactat gggccgtaat gctacaggcc taaagcaaaa cactggtgtt cagacagcga
     4501 ttgaaatgct atcagtttta gggttgttta tgatgggggt gcttgcggga aactatgtaa
     4561 aagtagaatc ttcattgaaa tttgcgatat ccggacgtga gtttgttatc caggaaattc
     4621 ttgatcaaat cgtgccaggg atattacctt tagctgtcgt aatgggtgtg tacttgttct
     4681 atacgaaaaa aggattgaaa gtcacacaag cattattatg gctgactgga atattaattg
     4741 tgctagcagg aatcggtatt ttataaaaag aagggaacgt gaataagatg ggtgtagtca
     4801 atttagcgcg tgtggatgag cgtttgatcc atgggcaagt aatggtaacg ttatctcaaa
     4861 aaagtggagt gaattccatt tttgttgtag acgaagttgt agcaaaagat aaatttatga
     4921 gagatcttta caaaaatgca ggaagccgta caggacaaaa aacaattgtc attacaccag
     4981 agaaagctaa attctattgg gatgaatatc aatttaaaga atacaattgt attttgatcg
     5041 caaaaacagt ccaaacgatt tacgacttag taaaacacgg aatccccatg gaagaattga
     5101 atattggagg gattgctcaa aaaaatcctg atacagatct tttagtgacg aaatctgttt
     5161 atttaaataa atcagatgct gaaaaattga aagaaatgaa tgaacaatat ggcgtaaaga
     5221 atatttattt tcaagccact ccatctgcac ctaaaacagg attaaaagat gtattgaaca
     5281 agtttgattt gtcataaaca aatgcttaga aaaaagataa agagggcgaa aaaatgaaga
     5341 agaacgagct gtttcgtgat tgggaatttc gttatcgcta tatttaccgt aagcgccgta
     5401 caaaaaagag taaacagcgt tttctttcag cattagtatc agatatttac agtatgagaa
     5461 cagatgtgac agtcattgct tacgatacgc tagcctaccg atcaaaaaat atttatgttg
     5521 gagatatcga aaaagcagag aaagttatct gtacttatta cgatacaccc gttcatgcat
     5581 taggaagtta tttcatgttt gattggaaag accaaaggaa aaaaacaatc tactctatat
     5641 tattgtcctt cattttgttg ttcagtttag gttggtgggg aatgatgata tataacaaaa
     5701 atcctcatca cgtattcgat ctgctatccg tataaacagg tatcactgta ctagcatttg
     5761 ggagctactt tttcttattg ggaaaagcag ctcgcggatg gcctagcaga caaactttta
     5821 ttcgaaatac ttcatccata ttaacgatgc tagaaatgat ccgtacgatt gatgatccga
     5881 gtgtagcgta tgcgtttgtt gatgaaggct gttatggaga aaagggctta gacagcgtga
     5941 gatcgggtat gaaaaaagag gctattcttt tttatctaga ctctgttggg gcagatacac
     6001 cgctacagtt ttcgggaaat tatttttcaa ataaagaaca gtggctgaaa caagttgata
     6061 agttgaaaga aaaaaacgtc aattatattt ttagtgcaag aaaaaagcaa gcgcagttct
     6121 tctatttgac caaaacagat ttacgaggga aaacatttaa ttggcaaaat gccaatcaga
     6181 ttattgctct ttttcgataa gtaaaaagtg caaaacaagg agtgagaaga ctaatgttgg
     6241 aaatcatgat cgttacacat ggtaagttga gcgaaggact gaaagatgcg gcggaagtaa
     6301 ttgtcgggac atcgagccat attgaaacaa tgtgcttaga gtctggggag gacatccaaa
     6361 cattcggaga gaaggtcaaa gaaaaattaa ctatgatcaa cacagcagaa cccatgctag
     6421 tatttgttga tctaattagc gcaagtccgt acaatcaagc gctcttggca gtcaatgaac
     6481 tgactgaaga aaagaaagca gccacttatg tcataggggg cgtgaatctg ccaatgttgt
     6541 tagaagcaat caatcatcag atgcttcaca caccgataga agaagcggtg gaacaaattg
     6601 tcaaacaagg aaaagataat ttaactgttt ggcatgcttc agcggaagaa aaaaaattag
     6661 atgaggacga tttttagaag gaaggctgaa aagatgaagt ggggatttag atggtatggt
     6721 gaaaaagggg attcgatccc attaaaatat gttcgccaaa taccgggaat aaatgggata
     6781 attggaactt tgctgactaa aatgcctgga gatgtttggg agatttcaga aattgaggag
     6841 ttgaaagcat ctgttgagag ccaaggactg gaactgcttg gaattgaaag tgtagcgatc
     6901 cacgatgcaa tcaaggcagg gacagatgaa cgagattact atattgataa ctatatacga
     6961 acaatcaaaa acttgtctcg atgtggggtt cgattgattt gttatagctt caaaccaatt
     7021 tttggatggg cgaaaactga tttgtttaat gaaaacaacg atggaagcta ttctctttta
     7081 tacgatcaga aaatcgtcga tcagatggag ccaggagaaa tgtatcaatt gatacacagc
     7141 cagtcaaagg gattcaaatt gccgggttgg gaagaagaac ggttgaaaaa attcgctcag
     7201 ctggaaaaac tgtatgaagg aatgaccgaa gaagaattat ttgaaaatct gacttatttt
     7261 ttagaaaaag tgattccagt gtgcgaaaaa gcagatgtaa aaatggctat ccatccagat
     7321 gatccgccat gggaactttt tggttttcct agagtgacga aaaatcttgg cgacttgaag
     7381 aaaattctaa atgcagtaga ttctccatat aatggactaa cgctgtgcac aggatcttta
     7441 ggggcaaatc cggaaaacga tttagttgaa atcatccatg aggtaggttc gcgcatccat
     7501 tttgtccact tcagaaacgt ggagttttta ggagaaagaa agttcaaaga aacggcacat
     7561 ccctctacag aaggctccct tgatatgtat catattatga aagcgttggt gcaagaaggg
     7621 ttcgacggtc ctatacgccc tgatcatggt agaaccgtgt ggggtgaagt ggctatgcct
     7681 ggatatggcc tgtatgatcg tgcaatggga ctcacttaca tgcaaggtct atatgaggcg
     7741 ataagtaagg aaaaaccact gggagagcat tatgaataaa atcaagctct tcatctttga
     7801 tatggatggc cttttattag aaactggacg tttagcttat cgtgcttatg taaaatcagc
     7861 acaaaagtat gattatgaaa tgcgtaagga agtctattat ttactaactg gccaaacaga
     7921 aatggcgata agagaacaaa tgggcatttt gtacggagaa gatgttccat atatcaaatg
     7981 gcgagaagca atcaatcaat ataaagaaaa aatcgtgaaa gaagataaga gagtatatac
     8041 gaaaaaagga gcagaagaaa ttttatcttt tgcaaaagaa agaggaatcc atacaatcgt
     8101 ggcttcgtct aatacacggg taaaagtaga aatgtattta aaaatggaaa atctatatgc
     8161 cttgtttgat caaatcattt caggggatga tgtaaaaaaa ggaaagccgg aaccagagat
     8221 ctttttgaaa gcttgttcaa aaatgaatat tcctccaagt gaagcacttg tatttgaaga
     8281 ctcgattgca gggatcgaag cagcaaggcg agcgggcatt ttgtcttttc tcatcaaaga
     8341 tcatttgata gggttgcccg atcataatgg gaagcataag ttgaaagtag atgtaagttt
     8401 atatgaatcc aataaagcag actatgaatt ttatgacctg catcaagcaa aacgattttt
     8461 agaaagtaaa gggttgtatc tctaataaag aggactattt tctagtcctc tttatttatt
     8521 gtcctgcacg actgagggaa cagatgaaaa agaagtaatt ttttattttg aagtatctac
     8581 cttatttgta gtgaacaatt tagaacaaac acaaaacgca tctactgtta tctaccagta
     8641 gatgcgttta aagagaagaa cgagaatcgt ccatatgagg gtttccactc cttatgcaga
     8701 tttttttaat tcttgcactg cttgttcgat cgtaacacct gtcgctacat gattggtatt
     8761 ttctttgtca aaaacgacaa atagattcaa atctgtatcc aaggttactc gatattgtat
     8821 atctttgtta tagaatgtca tagccattct cctttcttta aatacttgct gaaaggcaac
     8881 gaacgcgttg ataacgaaat tccatttata agtataccac caattgaaag gttttcatag
     8941 ttaagaaatt ttaaagtcaa aagttgcaga taaaagcaaa taaacaagaa aacaaaaagt
     9001 gaatcaatcg cggttattca actttttctt cgattcgaag acaatagaag acaatgaatt
     9061 ttatatttca ttgcattatc cttgtgttat tagaaaaagc atgatatatt aaatgagtaa
     9121 tctattttga aacgtaattt gagcgatata tctacataga taaaactcct tttgcctatc
     9181 aaatatttat tgcaacgaaa aaacgtacat tgatacgtat caggaggaat ttatttatga
     9241 ataacggtac agtaaaatgg ttcaacgcag acaaaggttt tggctttatc actggtgaag
     9301 atggaaatga tgtatttgca catttttcag ctatccaagg tgacggcttc aaaactttag
     9361 aagaaggcca agcagtgtct tacgatgttg aagatggtca acgtggccct caagcaacaa
     9421 acattgttaa agcttaataa aaattgactt ttaaccgtct tttcgaaaga aaagacggtt
     9481 tttttgtacg aaaaaattca ttggtcgttg aatgggctga atagtagtgt ataaaacaat
     9541 tttgtggagc gtgaagcttt tatggtagag gaaaatacga ataaagtgat gttgataaaa
     9601 agattcctat cttgaatatg aatcgagatg tcttgaaaat gaatgagttt gaccaatcat
     9661 tatcctatcg aaatttacag tttcgttcga ttggtctgtc aattaatgaa aaagtaaaaa
     9721 agaatatgtt ctaggaagag ggcggcacat ctaaattttt aagtaacgga cagttcaaat
     9781 atcaagcatt gaaagaggct atcctactgt ttgaaggaat acgaatatat aaaaacgaca
     9841 aagaggaaaa agtagttcat taaatccctt caagtgatga aagaatgaga tctaataaaa
     9901 cctgatcttt catctgtttt tttaataacg taataatttt tt
//
LOCUS       DARBER010000095         9889 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARBER010000095 DARBER010000000
VERSION     DARBER010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.099"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            72..3299
                     /gene="fss3"
                     /locus_tag="SCK06_002121"
     CDS             72..3299
                     /gene="fss3"
                     /locus_tag="SCK06_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG1152087.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3412..3726
                     /locus_tag="SCK06_002122"
     CDS             3412..3726
                     /locus_tag="SCK06_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1152088.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3739..4113
                     /locus_tag="SCK06_002123"
     CDS             3739..4113
                     /locus_tag="SCK06_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1152089.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4123..4458
                     /locus_tag="SCK06_002124"
     CDS             4123..4458
                     /locus_tag="SCK06_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG1152090.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4541..5881
                     /locus_tag="SCK06_002125"
     CDS             4541..5881
                     /locus_tag="SCK06_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1152091.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5959..6633
                     /locus_tag="SCK06_002126"
     CDS             5959..6633
                     /locus_tag="SCK06_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG1152092.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6626..6790
                     /locus_tag="SCK06_002127"
     CDS             6626..6790
                     /locus_tag="SCK06_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152093.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6866..8008
                     /gene="dcm"
                     /locus_tag="SCK06_002128"
                     /pseudo
     CDS             6866..8008
                     /gene="dcm"
                     /locus_tag="SCK06_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            7998..8288
                     /locus_tag="SCK06_002129"
     CDS             7998..8288
                     /locus_tag="SCK06_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152094.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8547..9731
                     /locus_tag="SCK06_002130"
     CDS             8547..9731
                     /locus_tag="SCK06_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1152095.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9728..9865
                     /locus_tag="SCK06_002131"
     CDS             9728..9865
                     /locus_tag="SCK06_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1152096.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3271 a         1669 c         2244 g         2705 t
ORIGIN      
        1 agactttata aacaaagatg ttcaagcgag catcttattt ttttgtgctt aatttgaaag
       61 gagaacattt catgtataaa aaacaaaaaa agaaattatg tgtattaact attttgagtt
      121 tggctgttca agcaattgtt ggagcaattg ctcctacgat tacttttgct gatgaaatta
      181 cccatccaca aacggtaacc gtagaactag atttagcaca tcaatatgct gttgaaggaa
      241 cttttagtga tggtcgtcca atgtcagaag tcactgttcc gcattatgca gtgtacaacg
      301 gctttaaaca agatattttc tgtattgaac caggagtccc gatttacaat gagttcactc
      361 ctggctatga gaaaaacccg ttaccagata tgtccgataa agccaaatta gtttccgttt
      421 tatggaaaaa agtaggtaca gatgcagata ctcatattgt ggcacagaag atgatctggc
      481 aagaagttaa tgggtatacc cttcactcaa taaaacgatc agatggtagt gccgtaaata
      541 tcgcagccat tgaggcaaaa ataaatcaag ccattgacga ttatcagaag aaaccaacct
      601 tccataatag tacggctaaa acagtattag ggcaatcgac tactgtgacg gatacgaata
      661 atctgaattt gtcagagttc gatgaggttg tggaaaatgc cgcaaacatt gattatcgtg
      721 tgaatggtaa tcagttgata atcaccccaa atgccaattc aaaagaaagt ggtgtgctga
      781 cgcttaagaa atctgctggt accggaacac ctgtcgccta taaaaaggct ggccaacaga
      841 ctttaatggc cggggcgatt gataaaccta atacctacac aatcaaaatt gatgtggaaa
      901 cggagggttc tttaaagatc aaaaaagtcg ataaagaatc gggtgatgtt gtaccaggaa
      961 cggttttcca tttagacttt ggaaaaactt tacctgcaaa agacgtgact actgacaaag
     1021 aaggcattgc tacattggat gagattcctc atggtacaaa agtaaccatt accgaaaaat
     1081 cagtaccggc gccttatacg attgatacca cccccatgac taccaccatt aaagcaggcg
     1141 aaaccattta cgtgacttca aaaaacgcac gggaaaaagg gcaaatcatt ctggataaaa
     1201 gcggagttga aacagggagt gatctttgga atgacaatta ttccttagct ggtaatacgt
     1261 ttgccattcg caaagacagt ccaaccggtg aaatcgttca agaaatgacc acggatgaaa
     1321 acggtcatgc ggaaacacca aaagagattg ctaatgcgtt ggaattggga acctattatg
     1381 tgaccgaaac taaagctagt catgggttcg tgaatacgtt caaacctgta aaagtcgaat
     1441 tgaagtatgc caatcaaacc gtagcccctg ttaccagtaa cgtaaaaggg caaaatcaag
     1501 aagtcactgg cgaaaccact ttgacgaaag aagacaaaga tactggggat aaagcacaag
     1561 gtaaggcagt ctttgagggt gctgaataca ccttgtttac tgctaaggat ggaaaagctg
     1621 ttaaatggag tgaggctttt aaaccagagt tagcgaaggg aacgaaaggt tctgatgaaa
     1681 cggtcacttt gactttagac gaaaagaacc aagttgccgt taaacattta gctatcaacg
     1741 agtattattg gcaagaaaca aaagcgcccg aaggatatag ccttgatgaa acaaagtatc
     1801 ctgtatccat caaaaaggtg gatgatagcg aaaaaaatgc cgtgattacc cgaaatgtta
     1861 cggcaaaaga acaagtgatt cgctttggct ttgatttctt taaatttgct ggatcagctg
     1921 cgggcactac cgaaactggc ttcaatgatc tgacctttaa agtgtcgcca ttggaaggga
     1981 ccaatgaaat cacaggtgcc gaagatgaag cgaccacagc ttataacgag caattagggt
     2041 ttgatggtta tggcaagttt gaaaatcttc cttatgggga ttatttactt gaagaagtag
     2101 aagccccaga aggttttcaa aagattacgc cattagaaat ccgttctact tttaaggaaa
     2161 acaaagaaga ctttgtgaag agtgagtatg tctttaccat taccgaacaa gaccaaaaac
     2221 aaccaattaa aacggtgacc gttccttacg agaagctgac gaacaaagca ttttctgtta
     2281 gtttgaaccg tttaatgctc tatgatttgc ctgaggaaga agatagttta acttctcttg
     2341 cgacttggaa agacggaaac aaagaattaa cttctctgga ttctaccgag ctagttgata
     2401 aattgagtta caatctacat gaaatcaaag aagactggta tgtggtagcg caagccattg
     2461 atgtggatgc gacaaaagct gcccaagaaa aagatgaaaa agccaaacca gtggtgattg
     2521 cagaaacaag cgcaacgttg gcgaacaaag agaaaacagg aacttggaaa attcagcata
     2581 aattaaccgc cgaacaagtt ttaaataaaa ctattgtctt attcaactat gtgtatgaaa
     2641 ataaggaagc ttttgaagca ggcgataagc cagtagcgaa ggatgttagc ttgaataacc
     2701 aagcccaaac tgtcagttgt acagtggaac accatgtttc tatccaaaca aaggcccacc
     2761 tagaaaacgg ttctcagacc tttactcatg gtgatgtggt ggatatgttt gatgatgtgt
     2821 cgattactca tgatgtactg gatggctcaa aagaagcttt cgaaacaatt ctgtatgctt
     2881 tattaccgga tggtacaaac aaagaaattt ggaaatctgg caagattgat tatgaagtaa
     2941 atgataaaga attcaccaaa actgtgcttg cgaaaaaagt agataccgga aaatatccag
     3001 aaggaaccaa gtttactttt gcggaaatca attacgataa agatgggaat atcaatggaa
     3061 aacacaatga agatttgaaa gaaaaatctc aaaccttaac accaaaagaa gttccaacca
     3121 tactaagtac gccgaaacaa ccggaaacac caactgttcc aagtggctct caagaatcta
     3181 gtcccacagt gaagaccttc cctcaaaccg gggagaaaaa ttccaatgtt ttactgttca
     3241 ttggttttac tttgattttt gcgacggcgg gctattattt ctggaatcgc cggaactaag
     3301 gtgatgagat gatacaagta aatcctaaga accaacgtgc gcgaccgcct cctaaaaaag
     3361 tagtcgcgct atttttgtat ccaaaactaa caatgaaaag aggaaaagaa gatggaattg
     3421 aaatttgtcg tgccagatat ggctgaaacc tttggaaagt taagttatgc cggtgaagga
     3481 gaaattataa cagaaggata tggacggaat actacggtga ttggtcgcag ttatcatttg
     3541 tattccagta agcaacgggc cgatgatatt gaggtggtag tagctgcgga agccggtgaa
     3601 aaggattttg atcaagacca accgctaaaa gccgtgaatc cccatttggt tgccaaaggc
     3661 tatgagattg agaatcgtgg gtttactgat tacgtgttgt atgttgatga tttagtgaaa
     3721 gcataggagg aaaagaagat gagattaaca gaaggtattg tcgtagattc aggtttaaca
     3781 tttgggaagt tgcgtttttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga
     3841 acaattggta atgaggtaaa agaacgaacc tataatttaa aatcctctgc acaagggcga
     3901 atgattcaag ttagtatccc agctaatgta cccttacgcg agtttgctta tgatgcagag
     3961 gtagagttgg tcaatccaat cgtggatacc gttgccaatt atgtttttcg agaaggaact
     4021 accgttaatt ggttcattaa ggcggatgat ttagtcttga agcggcagcc gaatcaagga
     4081 aatcctacga accaaaatga aagaaagaaa taggtgaaga ttatggaaat taatgttgaa
     4141 catattttag attgtctcga tcaatatgga aaaggcgagc tgactgagga acaactcgtt
     4201 aaagcattaa cctctgatga aaaaatgttt ctgattatac atcaaggttt acttggagaa
     4261 aatagcgaca gtgaagaaga ctctgatgta ctagactggt taggagaaga agagtctttc
     4321 tttatagttg tcgaagtaaa tgaaaactta tgtcggcagg cagaatccat tttggaagaa
     4381 attggtgtgg agatgccgga tgctattgaa ggctttttaa atcaattggt tgaaacgaaa
     4441 caactcccct cctcgtagca gtaaatgact agcataactt tttgaaattg cctaatgaaa
     4501 tcctattggg ctttttcgat tggaggtgga aaccatcaaa atgtacaaag gacatcgcat
     4561 acgcgcagga gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt
     4621 catcggttgg atgagcgtct tttattttca agagtttaga caatttgata tttctaagct
     4681 atcactttct actattgaaa tcgtttggtc tataaaagat ttagtatgtc tgcttggaag
     4741 tctcgctttt tcaggagcta tgatattgct ttatattcac ttttttcttg atcattggcg
     4801 aagcttgtgg catcgacaaa agctagctcg gatgattcta gagaaccatt ggtatgaagt
     4861 gaaacaaact caaagtgaag gctttttcaa agatttgaat agtagccgaa ccagagagac
     4921 tatcagttac ttccccaaaa tctgttatcg aatgaaagat ggattactgt ctattcgcgt
     4981 tcaaatttca cttgggaaat accaagatca gcttttaaaa ttggaaaaga agttagaaag
     5041 cggactgtat tgtgagttag tagaaaagga actcaaagac tcctatgtgg agtatacttt
     5101 actgtatgat atgatcgcca atcgaattgg gatagacgaa gtagtggcag aaaatgggac
     5161 tctgcgattg atgaaaaatc aagtatgggc ttatgattct ttaccccata tgttaatcgc
     5221 tggtggcacg ggtggaggga agacctattt cctcctcacc attatcgaag cactattgaa
     5281 gtcagatgca gaattgttta tcctcgatcc caaaaatgca gatttagctg atttaggtac
     5341 ggtgatgcct catgtttatt cacaaaagga agaaatttct gcttgtgtgg aagatttcta
     5401 tgaacgtatg atggctcgta gcaaggcaat gaaagaaatg tctaactaca aaacaggaga
     5461 gaattatgcc tatcttggac ttccacccaa ctttttaatc tttgatgaat acgtggctta
     5521 tatggagatg ttaacgacga aagaaagtgc ggtgattttg aataagctca aacaaatcgt
     5581 gatgttgggt cgtcaatctg gtttctttct gattttggct tgccaaaggc cggatgcgaa
     5641 atatttagga gacggaatcc gcgatcaatt caactttcgc gtggctttgg gtcgtatgag
     5701 tgaactcgga tattccatga tgtttgggga ggtcgaaaaa aacttcttta tgaagcacat
     5761 caaaggtcga ggttatgtgg atacaggtgg cagtgtgatt agtgagtttt atacgccact
     5821 tgtaccaaaa gggtatgatt tcttagattc aattagtaaa ttagaaaaga tgaatgattg
     5881 agtgatcttc aatactgact ttatacttca agtaacgtat aatactttta tatagtagga
     5941 agaaagggga taattaatat ggaaagtaat gattcttatt tagagtatgc aaaagcaaat
     6001 aaggataagt ttatttctta tattatcaaa gggaaatccc ccgaagttgg acgagacgca
     6061 gtttttatgg cagggagtcc tggggctgga aaatcagagg ttgccattgg attagctgag
     6121 aattatgata accatgtgat tattgatgcg gattactttc gcacccagtt tcctgattat
     6181 aatggaaaaa actctagtct ctttcaaaaa gcatcttctt ggttggtaga gcaggcattt
     6241 agatatgtct tagatcatgg ctattctttt attctggatg caacttttgc aatattaagt
     6301 gctgaaaaaa atatctcgcg ggcgttgaaa aaacattttc gagtgacgat tttctatgtt
     6361 tatcaagacc ctaaaatcgc ttggaaattt acacaagaac gtgaaattgc agagggacgt
     6421 catgtgccga aaaaggcatt tattaacgct tttttcaaat caagagagaa cattgcaaaa
     6481 gtaaagagta aacatccaga tgtattgctt catatcataa taaaagatta tacgaataat
     6541 atttccgaag ttcattatgc tacggataat ctagaattat ttttaccact agggtatagt
     6601 aggaaagatt tggaggttca attacatgac tgagaaagaa caagtaacga aaattgtaaa
     6661 gaaatacaat aagagcattg cggatttatc ggaaaatgct actgcaaaag agttcaaaac
     6721 ggtgattaaa tatgtagccg atcaggccaa tgaaaaacaa cggaaattag ttggattaaa
     6781 taaaaaatag taggacaaat taataatgta tgagccaaga gcaaaatgtt cttggctttt
     6841 ttctttgcaa tgaatgaggt aatgtatgag atttttagat ttattcgccg gtattggtgg
     6901 ctttcgttta ggtatggaac aggcgagcca tcattgtatc ggtttttgtg aaattgataa
     6961 atttgctaga cgtagttata aagcaattca tgatacaagt aaggaggtgg aaatgcatga
     7021 catcacaagt gtatcagatg agtttattca atccctcgga ccagtggaca tcctttgcgg
     7081 cggatttccg tgccaagctt tttcaattgc gggaaagcgg caaagatttt ccgatactcg
     7141 aggtacttta ttctttgaaa tcgctcggtt caccactctt ctccgaccta agcttttatt
     7201 ccttgagaac gtccgaggat tgctcaatca cgaaggaggg gctacgttcg agacgatcct
     7261 ccgaacgctg gatggattgg ggtatgacgt ggagtggtag gtgcttaact caaaggccta
     7321 cattccccaa agccgggagc ggatattcct tatcggacat tctcgagacg catgtaccga
     7381 acaagtattt cctatcattg gaccgtcttc aacatctgat caaaacatca gaaatttggt
     7441 aaatatcaat ccttccaatc gaggaatggg tggacaagtc tatggttcag atggcgtagc
     7501 gccaacctta acaggtgatg aaggaatcaa aatcgcactg ccagcaaggg atggaatctc
     7561 tgtagcagga ctattaccgg gaaagtttga acaaggtaat cgagtttatg aagttactgg
     7621 aaccgccccc accttgtcaa cgaaacaagg tggtacaaaa atcatgattc gaaaaaatga
     7681 aacagaatat caagaggtga agccagaaga cagtgtgaat ctggcttttc ctaattccac
     7741 aagtcgccga ggtcgtctgg gaaaacagag tgttcatacc ttactaacag gagaccaaca
     7801 agcgatcgtc acggatcagt atcaaattcg gaagctaaca cctagagagt gttggcgact
     7861 tcagggcttt cccgattggg cttttgatcg agattctcaa gtcaattcag atagtcagtt
     7921 gtataagcaa gccggaaact ctgtaacagt tccggtcatt tttgatattg ccagaagatt
     7981 aaaagagttg gatgtacatg gtttatgatg agttgattgg agaaatttat tgggtgattg
     8041 gaaagattca gtcagaccca gaattagaag aacaattgcg acggttaaaa tttgatattc
     8101 gcaagaatgg tgtcaaagtc gctggcgatc catatttaat gaatgaggag acggatgctc
     8161 gaattgaaat aaatcaagta attgcagaat ttgagcggat tgcggaccaa gcaaaagaac
     8221 cagatattcg tcaatacttg ttagaaatga aagcagagtt ggaagtaaat ggtattacag
     8281 atgagtaacc taaaccaaca caactgactg ttaggcgctg accgcggagg attcgcgaag
     8341 cagaaatcct ccgtggtgct gtgcctgttc atggacggcg ttagccgacc atgatgtctc
     8401 acccccgtat ctaacagggg ggtacaaaaa cacaaaaaat aagtaagcaa ccattaagaa
     8461 tgcttgttgt atcaaagata gtaagtaatt tgtataggtg ttaactcatt tgaaaaagtt
     8521 aacaccttga atttggaggt gagaacttgg cacaacggaa tttagattat cggctattaa
     8581 aagaccgacg caacgaatac ggcgtttcac aaaataaatt ggctacggct tgtggactta
     8641 gtcggccgta cttaaatcag atagaaaatg gtggcgtaac cgcatctata aaaaccatga
     8701 ggaaaatttt tgaccaactg gaaagcttta atcccgattt gcctttgacg ttactgtttg
     8761 actatgtaag gattcgcttt cccacaacgg atgcacgaaa aattattcaa gaaattctcc
     8821 atttgaaatt tgagtatatg ctccatgaag attatgcctt ttactcctat caagaacagt
     8881 atgtcatggg tgatattgtg gtgatgttgt ctcatgaaga agataaaggt gtgcttttag
     8941 aattaaaggg gcgaggttgc cgacaatttg aaactttttt actcgctcaa aagcgtagct
     9001 ggtacgactt tttcgaggat tgtctaaagg ccggtggcgt gatgaaacgc ttggacttgg
     9061 caatcaatga tcgagtgggg ctattggata tacctgagct aacgaaaaaa tgtcagaagg
     9121 aagaatgtat ctccttgttt cgtaccttta aaagttatcg ttccggggaa cttttaaagg
     9181 ctgatgaaaa ggacggcatg ggcaatacct tatatatcgg cagtctcaag agtgaggttt
     9241 atttctgttt gtatgaaaaa gactatgaac aatatatcaa gttagggatt ccattagacc
     9301 aaactgagac gaaaaatcgt tttgagattc gactgaaaaa tgaccgtgct tttcatgcga
     9361 ttcaagattt gttaaaaggt cgtagcattg agagcaccac gttttccatt atcaatcgtt
     9421 acttgcgatt tgcggataaa gtagaaggta aaaggcgaac caactggccc ttgaatgaac
     9481 aatgggggcg ttttattgga cggaatcgga aagagattca attaacttcc gagccaaaac
     9541 cgtataccat tgaacgaacc ttgaattggc taggccgaca agtcgcccct acgtggaaaa
     9601 tggctaagga gttggatcga ttgaatcgga caacctatat ccaggatatg gtacagaatg
     9661 cacgcttatc agaccgacat aaaaagattt tggagcaaca aagtatggca attgaaaatt
     9721 tgattatatg aaaagaggaa tcgtcatggg aattgtaaaa gatatttttt tggtatttgg
     9781 tggcagtatg cttggtgtta ctgtgatgtg cttgatgaat gcaagtgcag cagcggatcg
     9841 agcaatggaa aagaaaggaa agtgacaaga tgaatttcgg acaaaattt
//
LOCUS       DARBER010000096         9830 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARBER010000096 DARBER010000000
VERSION     DARBER010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.100"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..205)
                     /locus_tag="SCK06_002132"
     CDS             complement(<1..205)
                     /locus_tag="SCK06_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152097.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="SCK06_002133"
     CDS             complement(221..835)
                     /locus_tag="SCK06_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG1152098.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="SCK06_002134"
     CDS             complement(982..1665)
                     /locus_tag="SCK06_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1152099.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="SCK06_002135"
     CDS             complement(1774..2232)
                     /locus_tag="SCK06_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG1152100.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="SCK06_002136"
     CDS             complement(2316..4079)
                     /locus_tag="SCK06_002136"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG1152101.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="SCK06_002137"
     CDS             complement(4300..5760)
                     /locus_tag="SCK06_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152102.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="SCK06_002138"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="SCK06_002138"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG1152103.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="SCK06_002139"
     CDS             complement(6917..8344)
                     /locus_tag="SCK06_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1152104.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="SCK06_002140"
     CDS             complement(8375..9589)
                     /locus_tag="SCK06_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG1152105.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2917 a         2041 c         1628 g         3244 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DARBER010000097         9677 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DARBER010000097 DARBER010000000
VERSION     DARBER010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.101"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            42..779
                     /locus_tag="SCK06_002141"
     CDS             42..779
                     /locus_tag="SCK06_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1152106.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            793..1608
                     /locus_tag="SCK06_002142"
     CDS             793..1608
                     /locus_tag="SCK06_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1152107.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1621..2268
                     /locus_tag="SCK06_002143"
     CDS             1621..2268
                     /locus_tag="SCK06_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1152108.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2284..2940
                     /locus_tag="SCK06_002144"
     CDS             2284..2940
                     /locus_tag="SCK06_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1152109.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3102..3923
                     /gene="racE"
                     /locus_tag="SCK06_002145"
     CDS             3102..3923
                     /gene="racE"
                     /locus_tag="SCK06_002145"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG1152110.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3926..5281
                     /gene="rph"
                     /locus_tag="SCK06_002146"
     CDS             3926..5281
                     /gene="rph"
                     /locus_tag="SCK06_002146"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG1152111.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5282..5800
                     /locus_tag="SCK06_002147"
     CDS             5282..5800
                     /locus_tag="SCK06_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1152112.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5907..6407
                     /locus_tag="SCK06_002148"
     CDS             5907..6407
                     /locus_tag="SCK06_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1152113.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6687..7607)
                     /locus_tag="SCK06_002149"
     CDS             complement(6687..7607)
                     /locus_tag="SCK06_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG1152114.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7772..8785
                     /locus_tag="SCK06_002150"
     CDS             7772..8785
                     /locus_tag="SCK06_002150"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG1152115.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8895..9650)
                     /gene="fabI"
                     /locus_tag="SCK06_002151"
     CDS             complement(8895..9650)
                     /gene="fabI"
                     /locus_tag="SCK06_002151"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG1152116.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         3132 a         1700 c         2080 g         2765 t
ORIGIN      
        1 ttaaaagaaa tcttactaaa taataatggg agtgtaattc tatggcaatg attaaatttg
       61 atcacgtgga taaatattat ggcaagttcc acgcattaaa aaatatcaat ctcgaattcg
      121 agaaaggaga agttgtcgtt gtcattggtc cttctggttc aggaaagagc acgatgcttc
      181 gctgtatcaa tggattagag acgatctctt caggtaagct gctgattaat gatactgact
      241 tgcatgataa aaagacaaaa ctgacagaag tccgaaaaaa catcggcatg gttttccaac
      301 atttcaattt gtacccaaat aaaactgttc tggaaaacat caaattagca ccaatcaaag
      361 tgttgaagca agaccaggca actgcagtca aaaacgcgga gaaatttttg aaaactgtca
      421 atatgttaga taagaaagat tcttatcctt ccatgttatc cgggggacaa cagcaacggg
      481 tggcaatcgc cagaggttta gcgatgaatc ctgagatgct gctgtttgat gaacctactt
      541 ctgctttgga tccggaaatg attggtgatg tattagacgt tatgaaaaaa ttggctcatg
      601 acggtatgtc aatgattgtc gtgacacatg aaatgggctt tgccaaagaa gtggcggatc
      661 gtgttatctt catggctgat ggagaagtat tggaagacag cagaaatgtg agagactttt
      721 tcgaaaatcc aaatgaagtc cgagcacagc aatttatcag taaagttatc aatcattgat
      781 aggaggaaaa agatgcgcag gttaaaaaaa atacgcctga ttttcctttt aggcattgct
      841 tttcttgcac tagccggatg taaaggaaat gggttggcaa atgaagatat tttagagcga
      901 agcaaatcta ccaatgaaat catctggggt gtcaagtatg atactcgatt atttggaatg
      961 atggatattg agagcagaac tgtacaagga tttgatgtag atattgcaaa agcaatcact
     1021 aaaaagatcc ttggtgacaa tggaaaaaca gaatttgttg aagtcacttc taaaactcgg
     1081 attccattat tgaaaaacgg aaatatcgat gcaattattg caacgatgac gatcacagat
     1141 gaacgaaaaa aacaggttga cttttcagat gtctatttcg atgctggcca agctttgttg
     1201 gtaaagaaag gaagccaaat caaaagtgta gatgatttga atgcttctac aacggttcta
     1261 gcagtcaaag gttctacatc ggctgcgaat atccgacagc atgcaccaga tgcaaaaatt
     1321 ttagaattag aaaactatgc agaagcattt actgctttac aatctggtca gggagacgcg
     1381 atgactacgg ataatgcgat tttgttaggg attgcagatg aaaatccaga gtatgagtta
     1441 gttggtggaa cgtttactaa tgagccttac gggattgcga tcaacaaagg gcaagaaaac
     1501 ttcttgaaag ctgttaacca agcgctagag gaaatgcatg cagatggcac atacgataaa
     1561 atctatcaaa aatggtttcc aaatgaaact gaaggaaaag tagagtaagg gggagaggca
     1621 atgagcgaat tgatacaaac ttatggacca atctttgtcg acgggttcaa gatcacattg
     1681 ttttcgagtg tcttagcact tgtgtttagt ttgattatcg gaacgttaat ggcaatcttc
     1741 caattatcaa agaataaagt agtgagcgcc ttagcaaagg catatgtaga attttttaga
     1801 aacattccat tattaatcat cgtgatgttt ttttatgttg tcgtaccgat ttattggttt
     1861 aatctagatg gattccaagc aggtacgatt ggattaacga tctatacatc tgcttttatt
     1921 gctgaaacag tacgttcagg gattcaaacc gtaccaaaag gtcaaacaga agcaggacta
     1981 tcttctggtt ttacctattc agaaatcatg cgttatattg ttttaccgca agcattcaaa
     2041 atcgttatcc cgccattagg caaccaattt atcaacctcg taaaaaactc atctatttta
     2101 gcaatcgtag ctggtttaga tttgatgtat caaggagact tgatcgctag tgaaacattc
     2161 aataccttcg atacgtatat catcgttggg ttattttact tagttatcac gctaccgtta
     2221 tcttacttaa tggtttatct tgagaaaaaa tgggcggttc gttcatagaa ggaggggaaa
     2281 tacatggatt ttagcggcgc tttttcatgg atgaatatcc gttttttatt ggaaggatta
     2341 aaggttactg ttgaagtttc attgttttcg atcattttta gtttcttgat cggtggtctg
     2401 acaggtgtga ttcgttttgc gaatatccct ttcttttcca aaatccttgg tgtagtgatt
     2461 gatattatac ggaatcttcc attattattg atcattttct tcacttactt tgccttacct
     2521 caaatcggca ttcagatgaa catcttctgg tccgctgtcg cagcattaac gatttttgag
     2581 tcagctatgt tgtcggaaat tttccgagct ggactcaatg ctgttccaaa aggacagatg
     2641 gaagcaggat tatcaacggg actgacttat gtagaatcga tgcgaacgat cgtgttaccg
     2701 caagcattta aatcaatgat cccggcaatt gtgagccaat tgatttcact gatcaaagat
     2761 acttcattag cagtaatcat ttctttacct gaactttctc atcaggcacg tattatttat
     2821 ggacagaaca ccaattatgt gcttccgatg ttcatcatca tgacacttat gtacttcgtg
     2881 gtatgttacg cgctttctct gctttccggt tacttggaaa agagacggta cagttattaa
     2941 tagatgattc ttttagcggt tgtggcaaac taaagtcaca accgcttttc ctctttccat
     3001 tcgaaaaaaa gagatttcga ttgttttaag caagtaagag cacttgaaaa ttcacgctaa
     3061 aaagtacaca atagggagga tagaatttat tgaaaaaaag gatgatacga ttgacagata
     3121 atcgccctat cggatttatt gattcaggtg tcggcggctt gactgtagta aaagaagccc
     3181 tgaaacaatt accgaatgaa aatattttat ttgtaggaga cacagcacgc tgcccatatg
     3241 gccctagacc cgcggaacag gtaatccagt atacttggga aatgacggat tatctggtgg
     3301 agcaaggaat caagatgctg gtgatcgcct gcaataccgc aactgcggtg gctttagaag
     3361 aaatcaaagc tgctctttct attccagtca tcggtgtgat ccttcccggt actagagcgg
     3421 cagtaaaaaa aacacaaaat aaacaagttg gcattatcgg tacgattggt acggtaaaaa
     3481 gtcaagctta tgaaaaagca ctgaaagaga aagtaccaga attgactgtg acaagtcttg
     3541 cttgtcccaa atttgtttca gttgtcgaaa gtaatgaata ccattcatcg gtggcgaaaa
     3601 aaattgtggc agaaacatta gctcctttaa ccactaaaaa aatcgataca ttgattttgg
     3661 gatgcaccca ttatccatta ttacgcccca tcattcaaaa tgtaatggga gaaaatgttc
     3721 aactgatcga ttctggagca gaaacagtag gtgaagtatc tatgctgtta gattatttca
     3781 atttgagcaa ttcaccgcaa aatggtcgga cattatgcca gttttataca actggctctg
     3841 ccaaactttt cgaggaaata gctgaagact ggcttggaat cggacactta aatgtagaac
     3901 atatcgaatt gggaggaaaa taaggatgcg tcatgacggg agaaaagccc aagagcttcg
     3961 caaactggag cttcaaacaa atgttttcaa atatccagaa ggatcggtag tgatccgttt
     4021 tggtgataca acagttattt gttctgcaac aatagaaaat tctgttcccc cttttttgag
     4081 agaaacagga acaggatggg tgacagcaga atatagtatg ctgcctcgag caacgagtac
     4141 gagaaatcgt agagaaagca gcaaagggaa actttctggt agaacaatgg aaatccagcg
     4201 tcttattggt cgttccttgc gtgcagtagt agacttggaa aaattaggag aacgtagtat
     4261 catcgtagac tgcgatgtga tccaagcgga tggcggtact agaacagcaa gtatcactgg
     4321 ggcatttgtg gcattgaaat tagccattga aaaactttta agagaaaaag aattgtcaga
     4381 agatccaata aaagaacatc ttgctgcagt tagcgtagga atcttaccag atgggacttg
     4441 cgtcacagat ttggattacc aagaagattc cgcagcactt gtagacatga atcttgtaat
     4501 gacagaatct ggaaaattcg tggagattca aggaactgga gaagaagcaa cgtttgatgg
     4561 tgaacagttg aatgaaatgc tcttttttgg aaaaaacgcg attgaagatt tgatcaaaga
     4621 gcaaaaacat gctttgctaa cagagtttgc tcaaaatgat gagcggatag aagaaacaaa
     4681 aacaatcatc attgcgacaa gaaatccagg aaaagcagaa gagtttcgaa atatgttcaa
     4741 agaagcggga tatcatgtaa aaacattact tgactatcct gaattgccag atgtggaaga
     4801 aactggcagc acatttgaag aaaatgcccg attaaaagca gaaacgattg cacaattgct
     4861 tgatcagcca gtcttagcag atgattcagg tttgaaagtg gatgctttag gcggtatgcc
     4921 aggaatctat tctgcacgtt ttgcagggga acaaaaaagt gatgcgggga ataacgccaa
     4981 attattatat gaattgacag atgtgccgga tgaaaaaaga acagcacaat tccattgtac
     5041 gttagtattc gcggcaccca aaaaagacag ccttgtagta gaagcggaat ggcccggtcg
     5101 agtagcccgt ataccatctg gcgaaaatgg ctttgggtat gatcctttat ttatcccaga
     5161 aggtaagaaa caaacagcag cagaattatc gagcgaagaa aaaaacaaga tcagtcatcg
     5221 ggctcaagca atgaaaaaat tgagtgccga gtggaaacaa tggctggaag gagagagata
     5281 gatgcggtat ttagtagtaa gtgataatca tggagacaga gatatcctcg ttgacttagt
     5341 agatcgttat cgttctgaag tggatatctt tttccattgt ggtgattcgg aattagaagc
     5401 ttctgaccca ttatgggata cattccaagt ggttcaaggt aattgtgatt atggaccagg
     5461 attcgaacag aaaaaagtga tccaaactgg tcaggataca gtattcatga ctcacggtca
     5521 tttatcgaat gtccgatttg gtttgacaca gttagcaatc gaagctcgag ctgcaactgc
     5581 cgatatggtg ttttttggtc atacccatca aataggctgt gagatggatc agaaaatcct
     5641 gtttttgaat cctggtagca tttctcagcc tagaggcccg atccaaatcc catcatatgc
     5701 tcttatcgaa agtttagatg accaaatttc tgttcaatac tataaccgtg cccatcaggc
     5761 agttgaagag ttggcctttt ctttttcgag agagcgctga ataaaaaaac tctaattttt
     5821 aataaaaata agctttattt tatgaatttt gcatccggtt tcggtaaaat agtagtgttg
     5881 aatgaaatag aaatggaggc catcagatga ttggacctat agtaagagaa atgctgctgg
     5941 aaaatcagga aacgtttttg attcctgcag aaaatgtggc aaatgttatg tatcatcatc
     6001 cattgtccca tggtttactt gtgttatcga aggtcggtta ttccaaaatt cctgtattag
     6061 gaaaagatga tcactttgta ggactagtaa gtttatcaga tgtggtcaat aaaatgattg
     6121 acttgcaaac aatcagcatg gacccgttag aaggactaac ggttgctgat gtgacagaaa
     6181 cagatgttcc gacaattgat gaaaattggg aactagaaga agtactccat ttgttagtcg
     6241 attcgccttt tttaccggtt gtcacagaag atcggatttt caaaggaatc attacaagaa
     6301 aagagctgct taaagccgta aacttcatgg tgcatgaatt agaacgtcgt aatatattgt
     6361 ctccgaaaat cgaagaatta aaagaacaaa tcgatatcgt gagctaaaga acgagaaact
     6421 aaaagataat caaaaaataa gtagaaaaaa cagcgtacca gttttgactt tatcttgaaa
     6481 aagaggctgg gacaaaagcg tttaactcca agaaataaga aggaattcac gaaaattgct
     6541 tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgct tccgccgttt
     6601 atacgtgttt agagcgtggg acaaaagtgt tctttacttt tgtcccacgc tctttttagt
     6661 gagattcgtt gcatcgtttg tccaaattat tttccgacag caattggact gttcggaatc
     6721 gtgaaaccag aagctgtcaa ttctttgaca taggagctca agaactcttc ttttaaggca
     6781 tattgttttc cgttcaatgc gtagcagacc gtacgaatcg caaaattgct gtttccaaga
     6841 tccactaatc caaagataga aggttcagtt tggagctctt cttggtattt ttcagctaat
     6901 gtacgattta ctcgatcaat gatctcatag attttgtcat atccttcttc aggaacaatc
     6961 cgaatatcta aaagtacttg catatttgca cgagagagat tgctgatcgt ggtgatgttt
     7021 cgattcggga tgaaatggac agtaccatcc gttgctttca gctgaagggt acggatacca
     7081 acagatacga cggttccttc aatcgttaag tttgctagct tgatgtagtc cccaacatcc
     7141 atctgttgtt ccatgataat aaaaaatccg gtaataacat cactcatgaa cccttgggca
     7201 ccaagaccga ttgccacacc tgcaattccg gcaccagcca aaagtgatcc gatcggcaca
     7261 ccgatagcag ataaaattgc atagataaaa aagaaaccaa gggtataatg aaaaatagta
     7321 tttaaaagac tatgtaaagt ctttaagcgg ctctcgttta gaatggcttt tttagaatac
     7381 tgattaaaag acttatcaat cagatatttt cccacacggt gaagaatcca aaaaaggacg
     7441 atcaaaaaaa tcagatataa acttttttgg ataaaggcag ccaaaatggc gtcccaatca
     7501 atcccgtccc aaaaccgctg aaacgcattt actttgctga cagcttgatc tgtcaatgtc
     7561 tcgccagaag agtctgtcgt accggcagtt gctaataatg aaaacataaa atcctctttt
     7621 ctaaaatttg attctttctg tattgtacca agaagttttc aaaaaaggaa atagcaaaaa
     7681 taactataat gaaggaaaaa agctgaaaat aaaatgacaa ataatatgga taatgataaa
     7741 atgtggagaa aataacgaag gtggtaagta tatgcaaatc gattggaaca atctaggttt
     7801 ttcttatatc aaaactcctt ggcgttttat tgccaaatgg aaagacggac aatgggaaga
     7861 aggagaattg acagaagata attacctgac gattcatgaa ggctctccag cgcttcatta
     7921 tggtcagcaa tgttttgaag gtctaaaagc ttatcgtcga aaagacggca aggtcaatct
     7981 ctttcgacct gaacaaaata gtcgacggat gaatcaaagt gctagacgat tgctgatgcc
     8041 aatggtccct gaaaagatgt tcgttgatgc tgtcaagcaa gtggtaaaag caaatgaaga
     8101 ttttgtgccg ccctatggaa caggcgctac attatacttg cgccctttac tgattggggt
     8161 cggtgaaaac attggtgtcc atccagctcc agaatattta tttgtcgtct tctgtacgcc
     8221 agtaggagcg tatttcaaag gtggactaaa accaacgaat tttattgtat ctgattacga
     8281 tcgtgctgct cctaagggga ctggtgcagc aaaagtcggc ggaaattacg cagcgagttt
     8341 acttccagga acagaagcaa aagaactgga ttactcagat tgtgtttatc ttgatccagc
     8401 cacacatacg aaaatcgaag aagtaggtgc agcaaatttc tttggaatta caaaagacgg
     8461 aaaattcatc actccgcaat cgccctctat cttaccaagt atcacgaaat attccttgtt
     8521 gcagttagct gaagaacgac taggactgca agcaattgaa ggagatattt tcattgatga
     8581 tctagatcaa tttgcagaag ctggtgcatg tggtacagcg gctgttattt ctccaatcgg
     8641 tgggatttat cacaataaca atcttcatgt attttatagt gaaacggaag taggaccagt
     8701 aacaaaacgg ttgtatgatg aattgacagg tattcaattt ggagacatag aagcaccaga
     8761 aggctggatt gaagtcgtgg aataatttca aagtaatgag ttgaaaaaaa cgtaaaaaga
     8821 aaaaatcagg aagagacttt aaaaagtttt ttcctgattt ttatatgttt tactttaaag
     8881 aatgcaacaa aagtttatga aagatggaca cctttgtcaa cgtagataat atcaccgatg
     8941 attccgctgg aaagtgggct gattaagaaa gcacaagcat taccgacttc ttcgatcgtc
     9001 acaccttgct gatcaggcgt acgagattca gataactgga tcagtttttg atagtcttta
     9061 acacctgtga cagctaatgt tttgattgcc ccagctgaga ttccattgac ccgaattcct
     9121 ttaggcgaga actcagctgc caaataacgg atagcagctt caagagaagc tttcgcaata
     9181 cccatcatgt tataattcgg gattgcgcgt tctgaaccta gataagtcat gctaacgatc
     9241 ccgctgtttg gtgcaagaaa cggttgtgcg tattttgata cagcaattaa agagtagctg
     9301 ctgatatctt gagctaattg atagccgtct ctggaaatat cagacacatt tccggacaat
     9361 tcttctttgt tggcgtaagc aactgcatgg acaaggccgt ggatctcccc agcatattca
     9421 ccgatggcat tcattgcttt ttcgatgctt tcgtcgctag aaacatcaca ttctacagtg
     9481 aatgcttctt cgccgaccaa tttgactaat gattttttca ttcgttcatt ttggtaagta
     9541 tagataattt ctgccccttg tttcaacatt gcttcagcac aaccccaagc aatgcttttt
     9601 ttgttagcga cgcccataat gacgattttt tttccttcta gaaatgacat aatgactctc
     9661 ctttgtttct gataaaa
//
LOCUS       DARBER010000098         9561 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARBER010000098 DARBER010000000
VERSION     DARBER010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.102"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SCK06_002152"
     CDS             complement(152..550)
                     /locus_tag="SCK06_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG1152117.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SCK06_002153"
     CDS             complement(547..1320)
                     /locus_tag="SCK06_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1152118.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SCK06_002154"
     CDS             complement(1317..2000)
                     /locus_tag="SCK06_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1152119.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SCK06_002155"
     CDS             complement(2167..3054)
                     /locus_tag="SCK06_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1152120.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SCK06_002156"
     CDS             complement(3051..3920)
                     /locus_tag="SCK06_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1152121.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SCK06_002157"
     CDS             complement(4057..5343)
                     /locus_tag="SCK06_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1152122.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="SCK06_002158"
     CDS             5616..6194
                     /locus_tag="SCK06_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1152123.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="SCK06_002159"
     CDS             6194..7123
                     /locus_tag="SCK06_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1152124.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="SCK06_002160"
     CDS             7120..8856
                     /locus_tag="SCK06_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1152125.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..>9561)
                     /gene="pheA"
                     /locus_tag="SCK06_002161"
     CDS             complement(8919..>9561)
                     /gene="pheA"
                     /locus_tag="SCK06_002161"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG1152126.1"
                     /translation="LSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQALAQSQQ
                     FLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAENIQDNEL
                     NQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINLSKIESRP
                     LKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEETRR"
BASE COUNT         2996 a         2024 c         1637 g         2904 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag a
//
LOCUS       DARBER010000099         9525 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DARBER010000099 DARBER010000000
VERSION     DARBER010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.103"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            147..1016
                     /locus_tag="SCK06_002162"
     CDS             147..1016
                     /locus_tag="SCK06_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG1152127.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="SCK06_002163"
     CDS             1225..2250
                     /locus_tag="SCK06_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1152128.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="SCK06_002164"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="SCK06_002164"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG1152129.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="SCK06_002165"
     CDS             3333..5036
                     /locus_tag="SCK06_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1152130.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="SCK06_002166"
     CDS             5038..7071
                     /locus_tag="SCK06_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1152131.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="SCK06_002167"
     CDS             7082..7522
                     /locus_tag="SCK06_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1152132.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="SCK06_002168"
     CDS             complement(7722..8849)
                     /locus_tag="SCK06_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1152133.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..>9525)
                     /locus_tag="SCK06_002169"
     CDS             complement(8852..>9525)
                     /locus_tag="SCK06_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1152134.1"
                     /translation="LLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTLLN
                     GIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYWQS
                     KVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDPGS
                     IDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAIYR
                     KLYEV"
BASE COUNT         3236 a         1646 c         1949 g         2694 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatg
//
LOCUS       DARBER010000100         9362 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DARBER010000100 DARBER010000000
VERSION     DARBER010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.104"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(93..761)
                     /locus_tag="SCK06_002170"
     CDS             complement(93..761)
                     /locus_tag="SCK06_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1152135.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="SCK06_002171"
     CDS             895..1182
                     /locus_tag="SCK06_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152136.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="SCK06_002172"
     CDS             1598..3136
                     /locus_tag="SCK06_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG1152137.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="SCK06_002173"
     CDS             3133..4098
                     /locus_tag="SCK06_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1152138.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="SCK06_002174"
     CDS             4085..5407
                     /locus_tag="SCK06_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1152139.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="SCK06_002175"
     CDS             5648..6391
                     /locus_tag="SCK06_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1152140.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="SCK06_002176"
     CDS             6406..7398
                     /locus_tag="SCK06_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1152141.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="SCK06_002177"
     CDS             7410..8147
                     /locus_tag="SCK06_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1152142.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="SCK06_002178"
     CDS             8137..8859
                     /locus_tag="SCK06_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1152143.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9362)
                     /locus_tag="SCK06_002179"
     CDS             complement(8943..>9362)
                     /locus_tag="SCK06_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1152144.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3069 a         1607 c         1972 g         2714 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
     9361 ta
//
LOCUS       DARBER010000101         9106 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARBER010000101 DARBER010000000
VERSION     DARBER010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.105"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1189
                     /locus_tag="SCK06_002180"
     CDS             <1..1189
                     /locus_tag="SCK06_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1152145.1"
                     /translation="VLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMP
                     LVTRLKKQRYTCKNCRTHWTAQSYFVRLSHSIADHVRYKIASLLTEKVSLSFIAKSCQ
                     VSLTTVTRTLKEFKSYLPKPSKKILPRVLMVDEFRSHASIEDKMSFICADGETGNLIA
                     VLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHM
                     NQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRASINHYEYKTWKSFRAPKYP
                     FLTEAMMIDRLLEFSAPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDGFREK
                     LQNLLTYEEGITSAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI
                     NVR"
     gene            complement(1368..1697)
                     /locus_tag="SCK06_002181"
     CDS             complement(1368..1697)
                     /locus_tag="SCK06_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1152146.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1687..1959)
                     /locus_tag="SCK06_002182"
     CDS             complement(1687..1959)
                     /locus_tag="SCK06_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1152147.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2410..3213)
                     /locus_tag="SCK06_002183"
     CDS             complement(2410..3213)
                     /locus_tag="SCK06_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1152148.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3191..4315)
                     /locus_tag="SCK06_002184"
     CDS             complement(3191..4315)
                     /locus_tag="SCK06_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1152149.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4331..5146)
                     /locus_tag="SCK06_002185"
     CDS             complement(4331..5146)
                     /locus_tag="SCK06_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1152150.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5136..6008)
                     /locus_tag="SCK06_002186"
     CDS             complement(5136..6008)
                     /locus_tag="SCK06_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1152151.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6093..7382)
                     /locus_tag="SCK06_002187"
     CDS             complement(6093..7382)
                     /locus_tag="SCK06_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1152152.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7610..8254)
                     /locus_tag="SCK06_002188"
                     /pseudo
     CDS             complement(7610..8254)
                     /locus_tag="SCK06_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8731..>9106)
                     /locus_tag="SCK06_002189"
     CDS             complement(8731..>9106)
                     /locus_tag="SCK06_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152153.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2800 a         1947 c         1452 g         2907 t
ORIGIN      
        1 tgttctctcg cctactcctc gtgcttgtag aagttgtggt tctactgtgg tagatggaaa
       61 cgggaaagca attatagtga aaaatgggaa gaaggaaacg attgtccgtt ttgaacaata
      121 caatcatatg cctttggtta cgcgcctaaa aaagcaacgc tatacctgta aaaactgtcg
      181 tacccattgg acggctcaaa gttattttgt ccgactcagc cattcaatcg cagatcatgt
      241 tcgatataaa attgcttctt tactgactga aaaagtatct ttgtctttta ttgcgaaaag
      301 ttgtcaggta tctttgacta ccgttactcg tacattgaaa gagtttaaaa gctatttacc
      361 aaagccttct aagaagattc tccccagagt attgatggtt gatgaatttc gttcgcacgc
      421 ttccatagaa gataaaatga gctttatttg cgcagatggt gaaacaggaa acttaattgc
      481 tgtcttgcct acgcgtaaat tacctcgatt aacaagctat ttcttaggct gcaccaatcc
      541 agaagaagta gaattcttag tcacagacat gaacgccgct tacttccagc ttaccaaacg
      601 tgttctgcca aatgcgaaag tagtgattga tcggtttcat attgtcaaac acatgaatca
      661 ggcactcaat gagttgcgta tccgtgaaat gaatgagctt cgtaaagcag gacagaaaag
      721 tcaggcagaa aaactgaaaa agaactggcg ctttctgcta aaaaatcgtg caagcatcaa
      781 ccattatgag tacaaaacat ggaaaagttt ccgagcacca aaatacccat ttctcactga
      841 agcaatgatg attgatcgat tgcttgagtt ttctgcgcct ctaaaggagg cgtatccctt
      901 ttttcatgaa ttagtcgaag cctttcgaga caaagaccct gacttatttt tctccttatt
      961 ggcagaactt cccgaaacgt tagatgacgg ctttcgggag aagcttcaaa accttctgac
     1021 ctatgaagaa ggtatcacca gcgcaatgat ctatccttat tccaatggaa aaattgaagc
     1081 gaagaatacc cacataaaga caatgaaacg agtatcctac ggatttaaat cattcgagaa
     1141 catgagaatt agaatctttt tgatcaatca attaatcaat gtaagataac aaaaaatctg
     1201 aaccagaatg tggttcattc tgacccggat ttgattttgc acaactcatc agtccttatt
     1261 gacaaagagc caaaaatctg agccagaatg aggttcattc tgacccagat ttgattttgt
     1321 acaacccatc agtccttatt gacaaagagc ccttcttttt agtattatta taattcttcc
     1381 atgcgttctt ccagtcgctg taaatcgctt ggacttaatt ccccaatttt tttccatttg
     1441 acctttttgt tgtttttggg taattcgttt agagttccta cgtcaatcca agattctttt
     1501 tttagtcctg cctgtttcca atcccttatt tcaacgtatt tcagctttat tttatcggat
     1561 ttagtatgat attttgaagt aattgacaga aacgaaattg tgtccagtcc attttttaga
     1621 atatagacag gccggcgttt accgccctct gcagctgtga aagcaacgta gacggttgct
     1681 atatcattag tattcatctt cattgaacca ttcttcaatt ttttcgtcag tgtctagttt
     1741 ttggacaggc aatttttccg ctaatctttg aatattatgg attcgtttac ttttttccga
     1801 caattcaata gaaaatggga tttttccttg ctcaacaatt tgcttataaa aaacggtgac
     1861 ggctgttttg gggctcatgc ctagttcatc aataatgctt tctgcttcat ttttcaattt
     1921 ttcgtccata gaaatttgta acttagccat tttctccacc tctttctgac aaacacatta
     1981 tatcacggaa agagttcaaa tggaattaat acaactactt ttgaactcct tgaaatgcag
     2041 tgtatttatc taaatataag gaaaggatat agtgctaaat ggccttgata gatagaaaag
     2101 tttcaaaatc cgccacagcg ataattgaag cgaacactca acgacacaca gaacaggaaa
     2161 agactcctaa aagaaccaaa ttaactagga cgtgtctgaa aactccacaa agatgtggta
     2221 tgctctggta aaaaacgaat ggcacaacca cgaagaccga tatggttctt ggaaaacttt
     2281 ctccaccctt tttgtaattg gagggatagt ggtttattga tagcgatttt ccaagttctt
     2341 caagtggaac cagacattga gcaccacggc taaaaagtga atgaagtctt tatttttaat
     2401 caataatttt taatgattac cagtaattgg ttttttcatg cgccctattt ctatttgatg
     2461 tggagttaaa taatgctgat aagtaaaaat ttttccgtca attaaactgc cactagtgaa
     2521 tcctgttcta ttggtaaaaa gagaatagtc tgggtattca tctacaccaa atgataaaga
     2581 ttcagccata aactgttttt taccagcaaa ttcagcactg ctaccttgat ggatatgtcc
     2641 tacaaaaata cctaaaatat cactttcggc aatcaattca gcaaaacctt ccggaacttc
     2701 agttacaata gatggatctt cccacgacaa tggatgatga accaataaaa tcgttccctt
     2761 ttcagcaggt gtacccaacc actcttgtaa ttgaatcatc tgtcctatcg atatttttcc
     2821 ttcgtgctgg gtatcttgag cagaatccaa ggaaataatt cgtaacccat tttgattcgt
     2881 acaatggata tactctcccg tttcattgct ttcaacactc attccttcaa agaaaacact
     2941 gcgacgatca tgattcccac gacaaaaata gtaaggtgtg gttgggaaat attgatccaa
     3001 cattttccgt aagacttgat aatcagaaat ccccccttca tgtactaaat cacctgttaa
     3061 tagaataaaa tcgtacactg ttaacgagag ttcttgtaac aatttttcca atgccaactg
     3121 tggattttta attgttggaa atagttcagc agtaaacaag ttagtttgat agttttctct
     3181 aaaatggata tcagagagat gcaaaaagcg catggctatt ccctctttct tcaataaaat
     3241 aatcaggata tcctaaattt tcttgcgtct gataatcaaa aaaatgcatt ctttcaggtt
     3301 ctatgcggag attgactgca tcaccaatct gccaagtagg tttttcatta aggacaatca
     3361 tttctatacc atcgacttta acggtgtacg cataactttg acctaatagc tcacgatact
     3421 tcacttgacc gtgtaaatcg cttgtttctt ttgaaaaggt tacgtgttct ggtcttatgc
     3481 ctaaatacaa tgagccacta acctgaagaa gttgtttttg ccaagctgga ttcaatgtta
     3541 aaaattgact ggctaccttt aatgttcctt gatcataagt aactggcata atattcatgc
     3601 ctggcgaacc aataaaattt gcagtaaaag tatttttagg tttcgtataa acatttttcg
     3661 gcgtatcaat catctgaatt ctaccttcat tcaataaaac aatccggtca gctaacgtca
     3721 tcgcttcaat ttgatcatga gtgacgtaga caaaagtttg tttataacgt tgatgaattt
     3781 ttaccaattc ttttcgtgca tccaatcgta aacgcacatc caaatttgat aacggttcat
     3841 ctaataaaaa ataattactc cgtttaacca ccgcacgagc caatgctaca cgctgccgtt
     3901 gccctcctga caaatcttta ggaagtcgat cttggtatgg cgttaaacct aacatcttca
     3961 aagcttcttc caaacggtgc tgaatgtcag ctgtcttaat tttattcgcc cttaatgcaa
     4021 aaataatatt ctcctttaca gtcatgtgag gatataaagc ataattttga aaaaccattg
     4081 cgatctctcg ttctccacaa ggtagcttat ttgctagttg tgtacctaaa aaaagctctc
     4141 cactcgtaat tttttccaaa ccagcaatca ttctcagcaa agtagatttt cctgaaccag
     4201 atggtcccag taacacaatt cgttctccag cggccacagc taaatcaatt tcttttaaaa
     4261 tggtatgttc tccatatttt tttccgatat ttcttaattc aagcgtttct cccatgattt
     4321 gtcctccaat ttatttaata cctgcagaaa caaaagtccc gataatatat ttttgaaaaa
     4381 ttgcatacaa gactaacggt acaaacattg taattaaact aatcgccatt gctacggtaa
     4441 aatttgtccc accttcagaa ctaatatagg tctgcatcgc caacggcaaa gtataattat
     4501 ctactgtccg taaaattaat accggccaaa catactcatt ccaagaggta ataaaaaacc
     4561 aaataccggt agaagtaatc gcatgtctag taagaggaaa aacaatttta ctcatgacgg
     4621 ttgtttgttt gatgttatcc aatgccgcca catctagaag gctcactgga atttcccgca
     4681 ttgtttgcgt caataaaaaa atgcccatcg catcagcaaa ttgtggtatt atcacacccc
     4741 aagtagtatc aattaaatta agttttgcaa tcaacaaata attaggaatc atcgcaacag
     4801 taaaaggaat gaacatcgta ccaatgatga taaagtacca gattttcttc cctttgattc
     4861 ggcaataagt aaaactatat gccgcaaaaa aagctacgcc taacttaaca accgttataa
     4921 ttgtagcaat aaaaaaagta tttctcgtaa ttttgataat tggaagattt tcgaacaaat
     4981 agcaaaaatt ttccaaggta aacggctgtg gtatcaaact agtaatcgtc tgataggcat
     5041 ctttcaatgg cttaaaagaa ttggccataa taaacaataa tggatagata ataatgatta
     5101 tgatgaacat aaataagaga tgtggcaacc agcgtttaat tttcatagta aacccccttt
     5161 tccacaaatt taaatattac ccctaagaaa acagcaaata ttaataaagt taaaactgaa
     5221 attgctgaag attctccgac tttatacatt tcaaaagcct tttgataagc ataatagatc
     5281 acgtttgcac taccattatt cggtccacca cgagtgatga tcttaatcgg agtaaacaca
     5341 tactgcagac cttgaacaat ggtcatcacc aataaatata gtcctgtcga actactcata
     5401 ggcaacacaa ttgtaaaaaa aatgcgccag atgggtacac gatccaactt agctgcttcg
     5461 atcatctctt gatcaattcc cacaattgct gcaaaaatga caatgaaatt ataaccaaaa
     5521 actttccaag cagtaatacc tgcaatcaca gcaattaccc aaccttgtaa attcatccac
     5581 ataggcagtc ttaaaccaaa atttcccaac aagacagcaa ttggtcccga taccggattt
     5641 aatatccatg taaaaaccat ccccgccact accaaagaaa tcacagctgg aataaaaaat
     5701 aatggtttat acaaattctg ccctttttct ataactattc ctaataaaaa ggatgtaata
     5761 tatgggaaca cacagctcag caagacaaat agaacggtat aggacaaagt attcttcaac
     5821 actttatgaa agactggatc agtcagcacc tttacatagt tggcaaagcc aacaaattct
     5881 ttagtggggg ccaccatatt ccagtcaaaa aaactcaaat agatcgtata aatcaatgga
     5941 taaaaaacaa acacgcccaa caaaataatt gctggcgcca gatacaaata acctacatag
     6001 cgcttcattt ccgacagcct cctcatcctt atgtcaaaag cagacgacct tgtttttcct
     6061 ttcttacagg cgctgtcgtc tgcttgcttt gcttatttga gtaactcatt aatttccttt
     6121 tgagtagctt tcatcgtttc attgacggaa ttacctgata aaattttatc ccgcatatcc
     6181 aacaattttt gttcagcttc taatccagca tctccaggaa atgaactcca actgaccatc
     6241 ttatccatct gatcaattgc tggtatcatc atctgatttt cagctaagaa agcttttaat
     6301 ccattttggg cgtctgcaac atctttacgt ggtggtaaat aaccagtacc ttctgtccat
     6361 ttagctaaag attcaacact ataaagatat tttaagaaat cccaagttgc ttcctttttc
     6421 ttatccgttt gtgatgtaac agctaacatt gcgccaccag caggtagttt aacttttttc
     6481 ccttcccaac ttggtgactc aactgcttta gcagtaaaag tactattttt ttgaatgttg
     6541 tccctttgag caatagtggt atagaccatc gcgacgttac catcaataaa gctttgcaca
     6601 ccttgttccc acgtagtgtg taatgccgtc tggtctttta ccatcctttg atataattca
     6661 taagcagcga taccatcttt agaagcaaag gtagctttgc catcttttat tacacttgcc
     6721 ccattacttt ctaaaattgc ttgttgtgcc caattatcag caggttcttg aatatacacg
     6781 ccatactttc cagttttttc ttttattatc ttagaaaaag tttgaacttc ttcccaagtt
     6841 tttggaccat tttcatccaa gcccgctgct tttaatatat cttgatttaa atataaaacc
     6901 ggattactaa tggagtaagg aatacccact tgtttaccat ctttttcaga cagtgccatg
     6961 atattgtcta aaaaatgatc tttaaaataa tcagcattat ccggatccac ttcttctact
     7021 aaagattgag gattagtata tttgaaattt tctgcaaaat aatctttaaa agcccatccc
     7081 acttgtacta cgtccggtat ttctttggag gcaacagaag cttgtaaatt ttgcatcaag
     7141 cctttataca tatcaggatt atattttgct acaaccttca ctttgtcttg agattgattg
     7201 tatttatcta ctaattcatt cactgttttt ccacctaggg tatccgcatt aacatgccaa
     7261 tactctatcg ttacacgacc ctccgatttt tttgttgtct tggtattccc actaccacaa
     7321 gctgccaaag agatgacact taaaacagac acaaccccta taacaagatt tttctttaac
     7381 ataacattaa cttcctccta atttctttat ggccattttt ctaatcaaga aatattatga
     7441 actaccaatg taaatttcta gttaattttc tgtaaataat tcgaaatttg tgcatttcgg
     7501 ggtacagggt cactggacat atccatcaag ctaagaataa agcccccata tggtcatgta
     7561 tcccatctcc aaatctctta actcctctgt tttgattata aaatagttta ttctgtgctc
     7621 ctttcttcca taaataggcc catgcacaac gtgaggttat attataaaca ccacagcgcg
     7681 caaggtactc aatcgtttct agttcattga ctgtccatac agaaaatgtc aatcctagct
     7741 gctgccccac ttcacgggcc aacttagtta accatccttc atgaatattg acgatttgaa
     7801 cgctttgata ggctaatttc tgtaaggtat cgatcaaatc tgcttcggta atatccccaa
     7861 gttttttccc ttcgaactga ttttcaatat tataaaatat atgctttccc caaatcgcag
     7921 cccaagataa ttgtacatag ccactaagaa caatttgtgg ccagatttga taggtttgtg
     7981 ccagcgaaaa aacggccatt tccaatccct cttcttttaa atcacagtta atggtcatgg
     8041 ttgccttctc attggccact gcttgaaaca cctgctcaag ggttaccgaa tctgggacaa
     8101 cttcttacct gtcatgggct agtattagcg taccatctgt ttgctgacgc acatctactt
     8161 caaaacaaag ctggcgatcc aaacaaaagc gaagaaatgc ccagctattc tcctccgtat
     8221 tgtcactacc agagtgtgca gtgatcatgg tcattttgac ccctccttta attccgtgat
     8281 tttctcccac acgtccacta taccgatgaa gcgttaattt cttgttaatt tcttgtaaat
     8341 tccctgcgtt ttttcagtaa actagttgta aatcttgcat gctccttcct acgaactcat
     8401 gaatcgtcga ttttgccctt ctagctagtt tcaccaatta agtagttcta cattccccgc
     8461 ttttaaccta ctacaccttt ttttgttttc agctattcct tcttcttgct gaaagtcctt
     8521 gaggttcttt cttacaggat accatcaagg tgttcaatgg cttattccca ttgaatgacc
     8581 gagacttact atcagatttc tgctcgttga accaagtctt tgatacagga tttcttccgt
     8641 catcttctca caaataccac cttgcgtttc tctagcgatt ggtcgatgtg caccctccca
     8701 gtggactgtc actacccctt tttgctatgc ctagcccaca aaaaaagctg tcctcacaga
     8761 cagccagact gtagacaaaa gctgttccaa actcgtaact cgagcttgga acagcttttt
     8821 atctacttcg tttcgaattt ttgaacaaaa aaataaaggc tccttgcctt tccatagcca
     8881 ggtggctaat ttctttaaat tcattgcagc aaacactagc atcgtatgcg tcgcgacttt
     8941 ttccaagcct cgatacttgg tccaacgcat cccatgcttt tcttttgcat ccgcaaatat
     9001 tcgttcaatc gtttgtttcc gttttttgta aattgattta ttcagatctg ttaggcgaag
     9061 atgttccact tcatccatca gatctcccca aacatgtctc gtgatg
//
LOCUS       DARBER010000102         9048 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DARBER010000102 DARBER010000000
VERSION     DARBER010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.106"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="SCK06_002190"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="SCK06_002190"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG1152154.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="SCK06_002191"
     CDS             2714..2938
                     /locus_tag="SCK06_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152155.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="SCK06_002192"
     CDS             2951..3451
                     /locus_tag="SCK06_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1152156.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..5395
                     /locus_tag="SCK06_002193"
     CDS             3548..5395
                     /locus_tag="SCK06_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG1152157.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            5398..6294
                     /locus_tag="SCK06_002194"
     CDS             5398..6294
                     /locus_tag="SCK06_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1152158.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            6344..6733
                     /locus_tag="SCK06_002195"
     CDS             6344..6733
                     /locus_tag="SCK06_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1152159.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            6720..>9048
                     /locus_tag="SCK06_002196"
     CDS             6720..>9048
                     /locus_tag="SCK06_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1152160.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         3196 a         1362 c         1909 g         2581 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     4681 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     4741 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     4801 ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact agtatactag aattactaga
     4861 tggatataac tctgatttga tttcagagta taaaaatgag attgaaacat gtgtaaacga
     4921 aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa
     4981 ggtagagctt gaaattgaag atttaaaaaa acaagtagaa aagctatctc ataagagtta
     5041 taaagatgtt tatgatgagt attcaaaaga gacagatatt tatagggaac tgaaacgtga
     5101 tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat agtgttgaca aaattgactt
     5161 tggcaatggt ataaaagtta cagacgaaat tgatgaacac cgcggagaat tgattaagaa
     5221 tttagtgacc aatcgggcta agattcaaac aataattaat gaaatgtcag atgaaattga
     5281 tagttataga gcagagctga ataaatcaga ttggaaagtt aactataatg aggtttcaaa
     5341 gaggtatcaa agaggtatca aaaacttagt gaagagttat ctcaagatga attaaaaatg
     5401 ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa ctgaagagcg attggaagaa
     5461 attattcaac aagaaataaa gcttaaagat taccaagttg aggttgactc agcaatgtca
     5521 agaatgaata aatcatatca aaatctctat tctgagagaa gtagttcctc gcgcgaaatt
     5581 ttttctgata ttacaggaat agaggctact attcaaccaa atagggattt caataattat
     5641 gttaaagagc tcagagcttg tattaataag gaggacactt ttgacagtga gtttggatct
     5701 cttattgata agctgacaaa aaattccatt actaatcaag atatatataa tgacatactt
     5761 gatataagag ttagtgggga aagtagtatt ttttcagata aaaaattaat caattctata
     5821 agaaaattga atgatgcaca agttcttgaa ttacaaaccc ttaagccaaa tgatttagta
     5881 gttatccgac ttgaactaaa tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa
     5941 aagacatctg caattctttc tatgattttg gcgtatggaa attcaccact catattggat
     6001 cagcctgaag atgatttgga tagtcaatta attaataact taatcgtaag aagtatcatt
     6061 acaaaaaagg aaaacagaca aattttaatt gtaactcata atgcaaatat cccagttaat
     6121 ggtgattcag aatggttgat tagcatggga gattcaaaag aaatatcggc tgatcagtgc
     6181 gggtcggtag atgaacccaa tatcaagcca agaatatgta ctgtgatgga aggtggcgag
     6241 gatgctttta caaatagagc gaaacgctat ggttttaaac aaattgtaga ataacactag
     6301 agggtgagac atattattat gtccgccctt tttaggagga aaaatgaaaa aaataaaaag
     6361 ctataccagt atctgggcag ttgaaaaagt catttacgca atcaatgatt ttcagttgcc
     6421 ttttcctgtg acttttaacc aaatggcttg gttcgtttta tctttactgt ttgtcatcgt
     6481 atttgcccat gtgcctccac tatctatgat agaggaggtc tttttaaaat acttaggcat
     6541 accggtggct gtcacttggt ttatgtcgca aaagacgttt gatggcaaga aaccactggg
     6601 atttttacga tcctttatca gctatcactt gcgatttaag gtgacttttg caggaaagaa
     6661 agtcaaagaa cagaagaaac gatgggatga acccagtact cttgtaagga gtgtgaacta
     6721 tgtatccgat taagtatatt gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg
     6781 cctactatga attagtgcct tacaattact cctttttgtc acccgaacag aaatttcaag
     6841 tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga aggcaagatc catgcgttac
     6901 aaattgccac agaaagtagt attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg
     6961 gtagactcaa agaagtagcg aaacaacgaa ttgatataca gacagatgca ttagtatcca
     7021 tgattggcga tagtcagatt gattatcgct tttttattgg ctttaaacta attgcgacag
     7081 atgaagaagt caatctaaag agtctcaaga aatctttctt ttctgggctt caagaatttg
     7141 tctatggagt gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc
     7201 gctgctattc taagcttgaa aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca
     7261 ggcgagtcac accgagtgac ctgacgtatt tgattgaaca tatttatggc gaaaaaggga
     7321 taccgtttga agaatacgaa tttcagctac caaagaaaaa gctaaaatca gaaacactgg
     7381 tgaaacgtta tgacttatta cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct
     7441 tacggatgga acatgagaat catgagtcgt acgtagcgta tttaacaatt aatacaattg
     7501 tgggggagat ggaatttcct tcctcagaac ttttctatta ccagcaacaa caatttacat
     7561 tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg
     7621 ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca tgcttaccaa tctgacaatg
     7681 aaacgaattc caatgtctta gatgctttgg attccgtgga tgaattggaa accaccttgg
     7741 atcagtcaaa agaatctatg tacaaactga gttatgtggt tcgagtgagt gccgaatcag
     7801 tggatgaact aaagcgacgc tgtgatgaag tgctcgattt ttatgatgat accaatgtaa
     7861 aactagtccg gccctttggc gatatgatgg ggctacatga agaattctta ccctcaagta
     7921 aacgatatat gaacgactat attcagtatg ttacgtctga ttttttggcg gggcttggtt
     7981 ttggggctac tcagatgcta ggagaattag aagggattta ctttggctac aatgtggata
     8041 caggtcgtaa tgtttacctg aaaccggcat tggcttcaca aggagtcaag ggttcagtaa
     8101 ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca
     8161 atctcttagt ctactatgct gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag
     8221 gggagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     8281 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     8341 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     8401 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     8461 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     8521 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     8581 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     8641 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     8701 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     8761 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     8821 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     8881 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     8941 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     9001 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaacg
//
LOCUS       DARBER010000103         9021 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARBER010000103 DARBER010000000
VERSION     DARBER010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.107"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            331..1380
                     /locus_tag="SCK06_002197"
     CDS             331..1380
                     /locus_tag="SCK06_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1152161.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1377..2258
                     /locus_tag="SCK06_002198"
     CDS             1377..2258
                     /locus_tag="SCK06_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1152162.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2261..3031
                     /locus_tag="SCK06_002199"
     CDS             2261..3031
                     /locus_tag="SCK06_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1152163.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3310..4164
                     /gene="aroE"
                     /locus_tag="SCK06_002200"
     CDS             3310..4164
                     /gene="aroE"
                     /locus_tag="SCK06_002200"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG1152164.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4342..5505
                     /locus_tag="SCK06_002201"
     CDS             4342..5505
                     /locus_tag="SCK06_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1152165.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5622..6032
                     /locus_tag="SCK06_002202"
     CDS             5622..6032
                     /locus_tag="SCK06_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1152166.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6206..8434
                     /gene="topB"
                     /locus_tag="SCK06_002203"
     CDS             6206..8434
                     /gene="topB"
                     /locus_tag="SCK06_002203"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1152167.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8605..8889
                     /locus_tag="SCK06_002204"
     CDS             8605..8889
                     /locus_tag="SCK06_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152168.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3126 a         1538 c         1938 g         2419 t
ORIGIN      
        1 aaattcgatt agacttaaat caattcttaa ggaaagggtg acctgtaatg aacttatcaa
       61 aacgtatttc agcagaaaac aaaaaaaatt ggcgcagctg gcgtagataa gttgtattaa
      121 tggtgagact ttcccataga tacaactttt tagagtgcat ttctaaagct tgtatctatg
      181 ctagacatga acattgactg ccgcatagat tagaatctat gcggtttttt gtgtaaagac
      241 aatgaccgct ttggccattg tctttttttg tagactggat acttgcctga ttataataaa
      301 taaaaagatt gactttgcag gaggaaaaaa atgaaaaaca aacgattaat aacagttgta
      361 gtaattattt tagtttattt agcaggtgca tttatttatg gaaaagcctc aggcgaaaaa
      421 gaaagtactt cgacgaacca gacaaaaaaa acaacagttg ttggggtatt gcaatacgtg
      481 agccatccgg cattagatga gatttatcga ggaatcaaag atggtctgaa agaagaagga
      541 ttaaaagaag gagaaaatct tgagatcaaa tttcaaaatg gacaagccga tcagagcaag
      601 ctagccacaa tgagtcaaca gctagtacaa tctgatccag atgccttgat tggaatcgca
      661 acacctgccg ctcaatcatt agcaaacacg acttcatcga tcccagttgt gttaggtgct
      721 gtaacggatc ctgtgggggc tggattagtc gcttctttag agaaacctgg tggtaatgta
      781 acaggtgttt ctgatcagcc gcctgttact tcacaaatca agcttgcttc tgatttgttg
      841 ccaaatgcaa aaaaagtcgg aatcttgtat gcttcttcag aggataattc aaaataccaa
      901 gtagcacaag cagaagaagc tgcaaaaaaa gaagggttag aatcagtaaa atatgcggtg
      961 ccttccacta atgagattgc acaaacggtt caagtgatga gccgacaagt cgattttatc
     1021 tatgttccat tagataatac cattgccaat gcgatgcaaa cagtggtaaa agaagcaaac
     1081 aaagcaaata tccccgtcat tccatctgtg gatacaatgg tggaacaagg tggactagca
     1141 acaatcggga tcaatcaata tcaattaggg cttcaaagcg gtaagatggc ggcagaactt
     1201 gcgagtggaa aagaaaaacc ggaaacgacg cctgtttata catttgatca aggggataca
     1261 gtgatcaatc aatctcaagc tgatcgctta ggaatcattg ttccccaatc gatgaaggaa
     1321 aaagccaaaa tcattacaga tgactcgcag caagaaacat ctaaggagga tgacaaatga
     1381 tcgtatcagc tatttcacaa ggcatgcttt gggcaatatt aggattaggg attttcatga
     1441 cttatcggat cttagacttt cctgatatga cgacagaagg gtccttccca ttaggtgctg
     1501 ctgtctgtgt gacagctatt actcatgggg tagcgccgat tgtagctaca ttattaggcg
     1561 taggtgctgg catgatagca ggactcgtta cggggttact ttatacaaaa ggaaagatac
     1621 cggttatttt agcagggatt ttagtgatgt caggattgaa ctcagttatt ttgtttgtga
     1681 tgcgaacacc aaatctttct ttattgaatc atccgattct acaagacagc ttccatcgct
     1741 ttgggttacc tgattacttt gacacagtcc ttcttggctt catctctttg ggcatcttga
     1801 ttgctgtact gctattcttc ttcaatacag atttgggtca aggctatatt gcgacaggag
     1861 acaacgaaac gatggctcgg tcgttaggga taaaaaccga tcgtatgaaa attttaggtt
     1921 taactttgtc gaacggtgtc attgcattat ccggtgcgct gattgcccag aacgacggat
     1981 atgcagatgt aaataaaggg attggcgtaa tcgtcatcgg tcttgcttcc atcatcatcg
     2041 gtgaagtgat cttccaagaa ttgacgctag cagaaagatt gattgctatt gttgtcggca
     2101 gtatcattta tcagctattg attttagtag taatcaaact agggtttgac acgacttatc
     2161 tgaagttgtt ctccgctatc atcttagcta tttgtctgat gatcccgcaa ttgaagaaag
     2221 cactgaactt aaaaacgggt tttgagaagg aggcatagta atgactgata tattgacaat
     2281 caaaaatggc agaaaagtcg taaacaatgg aatgaatgaa gagaaagtac tattggatga
     2341 aattaacctt acaatcagag aaggggactt catcactgtc cttggcggga atggtgctgg
     2401 aaaaagcaca ctgttcaata cgatcgctgg tacgcttcaa ctcagtcaag gatcgatctc
     2461 ttttaaagat cagctgatca caaaagattc agaagaaaaa agagcacgat tcctttctcg
     2521 agttttccaa gaccccaaga tgggaacggc tcctcggatg acagtagcag aaaatttgct
     2581 acttgcacaa aaaagaggtc aaaaacggaa attccgttta cgtaacttga aaaatcaaaa
     2641 agaagatttt ttcagactat gtcaagaagt cggtaatggc ctagagcaac acttggatac
     2701 tccagcgggt gaattatctg gtggtcaacg ccaagcatta agcttgctga tggcgacgat
     2761 cacagctcct gagttactat tattagatga acatactgca gctttagatc ctaaaacagc
     2821 taaagcatta atgcgattaa caaatcagcg gatcaatgaa caaaaattga cttgtctcat
     2881 gatcacgcat cgaatggaag acgccttgaa atacggaaat cgtctgatcg tactccaaaa
     2941 agggaaaatc gttcaagata tttctgaaaa agaaaagcaa catttgacaa tgggcgacct
     3001 tttgcagttc tttgaagaag tagtggattg atttcgacag aaaaattcct ttcagtagaa
     3061 ggacttgaaa actcattgac atcttccttt gattcatcct atacttaaaa caataaacaa
     3121 ttaaacatat ttgggtgttt tgtaggatca cgtagtcaat gattttaaca attaaataga
     3181 atattctttg ctctgacaag taaacgatga gaaaagaatt tgctactttg ccacctagac
     3241 gtttcgtact ttttcgtatc aaacgctaag tggctttttt tattgaagta agaacaaagg
     3301 aggaataaga tgatttctgg acaaaccaaa ttagctgggc tctttgctga tccggcttct
     3361 cacagtttat ccccattgat gcacaatact gcttttgaag caaacaacat cgatgcggtg
     3421 tatttagctt tccaagtaaa tccaatgaat ttgaagcaag cagtcgaaag tattcgaact
     3481 tttgatatgt taggtgtaaa tctttcgatg ccgaataaaa cagcggttat tccttatctg
     3541 gatgaggtaa gccaggaagc gcaattgatc ggagcagtga atacgatcgt gcacgaaaag
     3601 ggtcgtttga ttggctataa taccgatggt gcgggcttca tgcaatccgt gcaagaagct
     3661 gggatttcga tcaaaaagaa aaaaatgact ctactgggag ctggaggtgc agcaaaggca
     3721 gtcactgttc aagctgcgtt agacggcgct gaggaaatcg tagtttataa acgaaacaac
     3781 caaacattcg ctcaagtaaa agaaacattc gaacaactca gctatcgaac ttcttgtaag
     3841 ataaaagttc gtgactttgc cgatacgcat caattaagaa aagacttgaa agaaagctgt
     3901 cttctagtca atgcaacaga tatcgggatg ggaaagaaaa tcaatcagtc accgctctta
     3961 gatataagca tgatgcatga gaatctagcg gtttttgatt tgatttatgc acctagagaa
     4021 acacgtttat taaaagaagc aaaaaaagta ggagcaaaag gatacaatgg acttggcatg
     4081 ttgatctacc aaggagcaat cgcgtttgaa ttatggacaa aggaaaagat gccgatccat
     4141 gtgattgaaa agctgtttga ttaaaaaagg ataatagata aagatctttt attctcgcat
     4201 aaacggtctc aagaaaagta atcctttatg tatcgaagca tttatttcgt tattttttat
     4261 taaattatca aaaaatgata ggcttttgtc gattggttat cgtatccttt atttatcagt
     4321 aagaaacgag gaggaaccac aatgagtcgg atggatcgct ataaggacat ccataagaag
     4381 gcgaaacctc tgaagcggga tacggggagt atttttgcta gaagagaacg aaaacgaacg
     4441 cgtatggaaa atgaggaaga tgctacccgt atttatgaaa gagagcagca ttcatttgaa
     4501 catttgaacc aatacgagca acagatatac agagatgcac caagagcaga agaacaacaa
     4561 aaaaataaaa aatttttcaa tcggcaaaag aagaacaaac cgttaaaacc tcgaagaaag
     4621 cgatcgtgga aaaaaatcat tctatcgatt ttgcttgttt tgatccttta ttcaggtatc
     4681 tcatttttta ttggtaaata tgtagctgaa catgacacaa gcttagaggc agcgacttca
     4741 gaaacctttc atgggcaaaa agcgacgaat ggtgcgcata atattttgat ccttggaagt
     4801 gatacacgcg gagaagatgc cgggcgagcc gatacgatca tggtcttaca gttggatggt
     4861 ccttcccata agccgaaact gatttctttt atgcgtgaca gcttcgttac gatcccaggg
     4921 gtaggacaaa ataagatcaa ttcagcttat gcatacggag gagccgatct agtgcgtcaa
     4981 actttagtag aaaattttgg aatcgattgt caatactatg cgaaagtcga tttcaaatcg
     5041 tttgaaaaag tgatcgatgc cttattcatg aacggagtga agatcgatgc agaaaaagat
     5101 ctgaatctgg atggtgtaga tattaaaaaa ggggtccaga agatggatgg acatgttctt
     5161 ttgcagtacg cacgtttccg aatggatgaa caaggagact ttggccgtgt cagacgccaa
     5221 cagcaagtaa tgaacgctat tttcagccaa ttgaagaatc cattgaatct gatccgtgca
     5281 ccttatgcag ctggtaaagc tttaggatat acatccacag atgtcactgc ttggttcttg
     5341 acgaaaaacc tcttttcgat tgcacgagga gctggtggca ttgatcgaat aagtgtgcca
     5401 gtagacggtt cgtggaatta tgggcaaagt gattatgctg gcagtattct tgaaattgat
     5461 aatcaaatga atcaatctgc tattacagat tttttgaata aatgatcata aaaaagatca
     5521 agaagaagct gcgcaaggct tttgtcatga atcaatcaaa cgattcactc gtaaaaaacg
     5581 ctttcttatg gtagatttaa ccaagagagg agctgtttac attggatatc tatctgaact
     5641 tcaaaaatga gtcgcgtgaa gccatcgctt tttatgaaaa agtttttcag actgaagcat
     5701 caggtatcac cacatacggt gaaatgcctg gaatagaaca aatgaacttg ccagaagaag
     5761 caaaaaatct aatcacgaat gcacggctga acatttatga tacattagtt atgtttgcag
     5821 atacgccaga aggaattggt cctgaattag ttgtgggaaa taatgtttct ttagtcatcc
     5881 agacaagaaa tgaagaagaa acgaagcgcc tatttgaaag cttagctgag ggtggtaaga
     5941 ttgagatgcc actggatgct gttccgtggg ctcagttgta tgggatggta actgatcgat
     6001 ttggcatcaa ctggcagatt aaccaagagt gacagctaaa gatgaatatt ttctggaaac
     6061 atcaagcagc aaacggatca tcgtttgttt gcttgatgtt tttgtgtcta tttcgactat
     6121 acggtatctt ttttttattg ccagatgcga aaatatgttc cataatagag aagtccgcaa
     6181 aaaattaagt aacggaggtc agaagatgaa gacggtgata ctagctgaaa aaccttcaca
     6241 agcaaaagcc tatgccgaaa ccttccaaca gcaaaagcgg catgaagggt attatgaaat
     6301 cagcgatcca ttgtttgctg gtgaagtaac catcacttat ggttttggac atttggtaga
     6361 tatggttgct ccaggagctt atgaagagcg ttgggcaaaa tggtctttag aaaatttgcc
     6421 tattttccca gaaaccttcc gttacgaagt tccaatggac aaacgtgcac aattttcgat
     6481 cgtaaaacga gaattgcagt cagccgatac catcattatt gcaacagatg gtgatcgaga
     6541 aggtgaagcc atcgcgtggt cgattattcg acaagcaaac gcattttcta aggaaaagaa
     6601 ctatttacgt ttatggatca attctcttga aaaagaagca atctatgaag gttttaagaa
     6661 tctccaacct ggaaagaact attttccaaa atataaagaa gcgcaagcaa ggcagaacgc
     6721 tgattggctg attggaatga atggaagtcc attatatagc ttgctacttc aacgaaaagg
     6781 gattgatggc agtttttctc ttggtcgtgt acaaacaccg acactctata tgatttatca
     6841 gctgcaagaa gaaatcaaac acttcaaaaa agaaacgtac tttgaaggag aaggtttaat
     6901 tacgacaaac aaagggcagt tttctggaaa aatcgtacca aaaaaagcat ttaaaaccca
     6961 agaagagtta acgaaggaaa tagaaaaact tggagcgcat ttaggtaagc aaaacggacg
     7021 gattcttgaa gttacgaaaa aggaaaagag gttgcatagt ccacgtttat tttcgctatc
     7081 tagtttgcag acaaagatga atcagctaat gaaagcaagt gccaaagcta cacttgatgc
     7141 tgctcaagga ttgtacgaaa gcaagtttct aagctatcca agaacggatt ccttttacat
     7201 tacagaaaat gagcatcagt atctggttaa acatctgcaa aattataaaa aatttttagg
     7261 aatagaagaa gtacagacaa ctgagatcaa agcaaagaaa cgatatgtcg atgcgaaaaa
     7321 agttcaagaa catcatgcaa ttattccgac aaagacgatt ccgactcctg cacgctttgc
     7381 gaagctttcc aaattacagc aagcaatcta tttgcaagtt ttgcgcacta ctgtggctat
     7441 gtttgctgca gattatatct acgaagagac gaccgtaatg acgggcgtac agcaattaaa
     7501 gctaaaaagc atcggaaaga tcccattaca gcaaggttgg caggaaatct tacgcatcaa
     7561 gtcaaaaaag aaagaagctc agactcttcc tcctatcgta gaaaatgaag aagcagaagt
     7621 agaactgaaa agtatggaga aagaaacaca acctccaaaa ccctataccg aaggaacgtt
     7681 gatcacagca atgaaaactg cgggaaaaac gctggataat gatgaagcgc aagaaattct
     7741 caaagaagta gaaggaatag ggacagaagc tacacgagcc aatatcattg aaaatcttaa
     7801 gcagaagcat tacatcgaag tcaataaaaa cgaaataact gttacgccaa aaggaatcac
     7861 tctttgtaag gcagtagcca atgaaccatt actgacgagc gcagaaatga ctgcgcgctg
     7921 ggaagggtac ttgagaaaaa ttggtaaaca agaggggaca ccatctgttt ttttagagaa
     7981 tatcaaaaaa ttcatcctac atttattggc agaagttcct aatcaaatcg acaaggtaga
     8041 cttttcagat gagattagcg ggacacgtca gataaaagcg atggagaaga aaaacgatca
     8101 attaggaaga tgtccaaaat gtaaaaaggg aatagtcatg ctttatccta aaatcgctac
     8161 atgcttgaat ccggaatgcg acttcaaatt gtggccaaca gtagctaaaa agaagctgac
     8221 taaaacgaat ctcagagatt tgctttcaaa aggaaagaca agtaaagttg tcaaaggttt
     8281 tactggtaaa aaagggaaat tcgatgcagt tttagtatta aaagaagata tgacaatcgg
     8341 tttttctttt ccatggatcg aaaacagtgg aacaaaagca gttgatggaa acaaagaaac
     8401 atcaagaaaa aataatgaaa acgaagtaga gtaaatgaaa acaatgaaat agcggaatat
     8461 ttattccgaa aagtgagaat ataaatacca ataaaactaa aaaatatcag tgaatcaagc
     8521 aaaaacgttg gggtaggctg attttatata gatagcaagg agttttttta tttgaaaaaa
     8581 gtgcattcat ttttaaatta tcttatggga gcaatcacga ttgtggctgt gtaccaagca
     8641 tttctaaagt atcctgatta cgggacgaag ttgttgtcat tgacgcacaa tcaaacagca
     8701 tattcaccaa cgttgatttt atcgactttg actgcaggag ctaaaagcgc tacttatttt
     8761 tttcataaaa cgacaagcat gctaaagcga aagaaacagc gaaaacaatt gttggtggtg
     8821 gtagcggcta tgatcattat ttattttcta ccagttatta cacaagtatt tcatcaatat
     8881 tttggttaag aatacgagag tttgtgacaa aagtcatgtc acaaactttt ttctttgtca
     8941 ttttttcgta agaaaaagca aaataaaagt atatacattg attaaaagta tatacttgga
     9001 tataatgtac ttactataga a
//
LOCUS       DARBER010000104         8924 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARBER010000104 DARBER010000000
VERSION     DARBER010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.108"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            139..1200
                     /locus_tag="SCK06_002205"
     CDS             139..1200
                     /locus_tag="SCK06_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1152169.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1203..2099
                     /gene="murQ"
                     /locus_tag="SCK06_002206"
     CDS             1203..2099
                     /gene="murQ"
                     /locus_tag="SCK06_002206"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1152170.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2113..4095
                     /locus_tag="SCK06_002207"
     CDS             2113..4095
                     /locus_tag="SCK06_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1152171.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4160..4606)
                     /gene="nrdI"
                     /locus_tag="SCK06_002208"
     CDS             complement(4160..4606)
                     /gene="nrdI"
                     /locus_tag="SCK06_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1152172.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4817..6241
                     /locus_tag="SCK06_002209"
     CDS             4817..6241
                     /locus_tag="SCK06_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1152173.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6422..7195)
                     /locus_tag="SCK06_002210"
     CDS             complement(6422..7195)
                     /locus_tag="SCK06_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1152174.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7192..8097)
                     /locus_tag="SCK06_002211"
     CDS             complement(7192..8097)
                     /locus_tag="SCK06_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1152175.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8251..8571)
                     /locus_tag="SCK06_002212"
     CDS             complement(8251..8571)
                     /locus_tag="SCK06_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG1152176.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2815 a         1593 c         1886 g         2630 t
ORIGIN      
        1 tgacaattct tttgataata taaactcaac ctttagaaat gaaatttaat tattttaata
       61 aaagtattga aattttgttt ctttagatat atactctagt catagaaatg aagacggata
      121 cagaaaaaga gggataatat gtttggcttt tcaatattta tgaacgattc tttgtcagaa
      181 gagaagaaac agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc
      241 atgcatatac cagaagatga tgtttcggca tatcaaaagc gattgatcga actaggaagc
      301 gaagcgaaaa agaataaact agaattgatg gtcgatattt caggagaggc attggaacga
      361 gcgggttttt cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga
      421 atggattatc atatttccaa tcagcagatt gccgactggt cacatcaatt gaaaatcagt
      481 ttgaatgcta gcacgatcac gcctaaagat atcgatgaat tgaaagaagc agaagctgat
      541 ttctcacaga ttgaagcttg gcataattat tatccacgtc ccgaaacagg gctggacaaa
      601 gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt ccaaggtttt
      661 gtaccaggag atacagagtt gagaggccca ctatatcaag gattgccaac tttggaagaa
      721 catcgaggga tccatcctct tgcaggcgct ctagatctat tagcaagtaa cacggatatt
      781 gtttacatcg gagatgcagg tctgtcagaa aacgttcagg agcaatttgc ttctttccaa
      841 aaagaacaga cagtcttgct ccatacggaa cctgtagatg aagaatttta cgaatatata
      901 ttagggaagc atacgaatcg gcaagacgat gcaagagatg tcatacgaag cgtagatgca
      961 cgttttcgag agattccgcc tatcccagca agaaatacag ctacgcgtat gaaagggtct
     1021 attactttag ataacgaaaa gtatttgcgc tatatgggtg agatccaatt aaccaaatat
     1081 gatttgccag cggatgaaaa agtgaatgta gtagctaaag ttatcaaaga agagctacca
     1141 ttgatcaacc aaatcaaagc tggtatgaac tatcaattta taagaaaaga aggaagataa
     1201 aaatgagcaa aatcaattta gagaacctaa caacggaacg cagaaaccaa gaaacttttg
     1261 gattagatga gatgagtatt tctcaagccg ttcaactaat gaacaaagaa gatcagaaag
     1321 tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc agtgattgct aacacgattg
     1381 agtcctttaa aaaaggtgga cgtctgattt atatgggagc cggaacaagt ggaagactag
     1441 gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt agaaccggaa atggtcatag
     1501 gtctaattgc tggtggagaa gaagcaatga ctgtagctgt cgaaggggcc gaagattctt
     1561 atgagctagg gcaagaagat ctaatcaagc tgaatttgac aaaaaatgat atagttattg
     1621 gtatcgcagc tagcggtcgt acaccatatg tgatcggtgg attagattac gcaaaatcga
     1681 tcggtgctgt cacaggaacg atttcttgca ataaacaagc cgaaatcagt aaacacgcgg
     1741 atttcccaat cgaagtcgat gcaggtccag aatttttgac aggctctact cgattgaagt
     1801 caggaacagc acaaaaactt atcttgaata tgatttctac tatcagtatg attggtatag
     1861 gtaaagtatt caataacttg atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac
     1921 gctcaaaacg aatcatcatg caagcgacag aagctgatta cgaaacagct gaaaagtact
     1981 ttgaagaagc agatcaaaat gttaagctgg ctatcgtcat gattttgaca aatagtgata
     2041 aagaggaagc tcaagcaaaa ttagctgaag caaacggatt tgtaaaaggg accttatgag
     2101 ggggaaaaga ccatggcaaa aaacgaactg actttggaag aactggcaaa acaaatttat
     2161 aaaggggcag gcggcatggg caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt
     2221 atgaagatca tcaatgatga tcttgtaaat gtctctgaat taaaagcgat tccaggagtg
     2281 cttggtgtag tagaagatga acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa
     2341 gtagcacagc aaatggttga tttggcaggc gtgaaattag gagaaaaact ttcttctgta
     2401 agtacagcta cagcaaattc atcagcatct ggaaaagacc gtgtaaacga acgggcacaa
     2461 gaaatgaaag cggcacaaaa atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa
     2521 gacatttcta atatctttgt gccaatgatt cctgcatttg tcggtactgg gatcgtggca
     2581 gggattgccg cagttttagc aaacttagtc acagccggaa cacttgatgc agcaacatgg
     2641 caacaatata tcgatgtgat gaacatcttg aaaaacggga tgtttagcta cctagttatc
     2701 tatacaggta tcaatgctgc acaagttttt ggtgcaacac caactcttgg tggagttatc
     2761 ggggccgttg tgatgctgac agggatgaat ccggaagcac cactgaaaaa cttatttact
     2821 ggtgaagctc ttgcagctgg acaaggtggg atcctaggtg tcatcttcgc tgtttggcta
     2881 ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg atgcggtaga tatcatcgtt
     2941 acaccaactc tttcattgat tgccatcggt ctgattgaaa ttttcttcat catgccttta
     3001 gcaggtttca tttctgatgg catggtcggt gggatcaact gggtacttgg cgttggtgga
     3061 gcatttgccg gcttcgttct aggcacatta ttcttgccga tggttatgtt cggactgcat
     3121 caaattttga ctccaatcca tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt
     3181 ccaattcttg cgatggctgg tgcaggacaa gtgggtgcag cattggcatt atggattcgc
     3241 tgcaaaaaag ataaagaatt aacagaaatg atcaaaggag ctttaccagt cgggatccta
     3301 ggaatcggcg aaccattgat ttacggagta acattacctc taggacgccc atttattact
     3361 gcttgtatcg gtggaggtat cggcggggca gtgatcggaa tgatcggaaa tgtcggggct
     3421 atcgctattg gaccttcagg agccgctttg atcccattga tctctgacgg taaatggtta
     3481 ggctacatcc ttggattatt ggcagcttat gctggtggct tcgttgctac attcttcttc
     3541 ggtattccta aagaccgttt agaaaaagaa gaattagcga ctgaaacagc cacagtaaat
     3601 gaagtgcctg aaaaagtagt acctcaaact tcagctgaaa gtgtgactat cctctcatca
     3661 gttgctgacg gaacagttgt ttctttagat aaagcaagtg atccagtttt tgcacaaaag
     3721 atgatgggtg aaggctactt tgtagaacca gaaaacggac atatctattc tccagttact
     3781 ggggtagtca gctctgtctt cccaacgaaa cacgctattg ggatcacaac gccaaatggt
     3841 ttagaaatct tgcttcatat gggaatcaac acagttgact taggcggtac gccatttgat
     3901 gtgaaagtaa cagaagggca gcaagtgaca cctgatacgc ttgtagcaga agctgactta
     3961 gaagcgatca aagcagcagg caaagaaaca tccatgatcg tcttggtaac caatatggat
     4021 cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg
     4081 gatgtcaaag cttaatatga tagaaacaat agaaacgaaa gaagaaactg aaatctattt
     4141 ttcagcttct tcttttttat tatttattgt tttctgctaa aatctgataa attcgtgtca
     4201 catcagatgg tgttccccgt aattcataat ctgccaagaa aggaaagcca aatgtttgtg
     4261 catattgttt ggctgttaga caatactgat tattgaagtt tttatttccg ctaccgacta
     4321 caccaagaca aagtttatga ttgtcttcat gttccagata ttcacgcatc gtttcggtca
     4381 agatttctgt atcaccgtta tctaaaccat ttccgccgtc taaataagta ggaacgaaag
     4441 taaaaaatgg ttctgtttct ttcgtaaaat cactattttc atggatttct tttaaagtaa
     4501 cttcaggatt gacaggatct tctgcatgca ttttttcagc atattctgct agatgtttgg
     4561 caaaggcacg cgtatttcct gaaatcgata tatataaaat gttcatggat catcctccaa
     4621 ttctttcctt ttagtatagc atctttttta agttttttgg tggtgtaatt tggaaaggga
     4681 ctaggcgctt tgcttgtata atgagaaaaa agccggtaga attatatttc tattaaagtt
     4741 tttattaatt aagtaaaaat aaaaaaggtt tgttatactt tttgataaaa gcggttttat
     4801 tttggaggat gcttacatgg atacagagca acagaaaaaa ggaattggtt taattccact
     4861 tgctgcctta gtaattggtt cagcgattgg aggtggcgta tttggtatta tgacggacat
     4921 ctccggatcg gctggcggcc cagctatttt cagttgggta ctcgtaggaa cagcaatgtt
     4981 gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt ccagatttaa aaggcgggat
     5041 ctttagttac gcagaagaag gttttggccg tttcgctggt tttatcagcg gttggggtta
     5101 ctggctcaca tgctggttag gcaacgtagc ttttgcaaca ttgttgatga gcgcattagg
     5161 atatttcttc ccagtatttg aaggtgggca aaatctggca tcgatcgtga tgagtacagt
     5221 ctttttatgg ggatatgcat ggctagtgaa tcggggagtc gaaaatgcta gtttcgtcaa
     5281 tgctatcgtc acattctgta aattagtgcc attattttta tttattgtag tagctatcag
     5341 cacgttttcg atccaacaat tcattgataa cttttctagc gacattatat tgaatgcgaa
     5401 tggacagccg atcccgtttt ccagtcaagt attgagttgc atcatggtga tgctttgggt
     5461 atttgttggt attgaaggag cctctgtcgt aggttcacga gcaaaaaaac aatcagatgt
     5521 tgggaaagca acgattctag ggatattcgg cttgattatt atttacgtct tggtttccat
     5581 gcttccatat gggacaatga cgatgaatca gctgcaaacg atcaaggcgc agcctgctat
     5641 gggctatgtg tttgaaatga tggttggtaa atggggagca gtcgtgatca acggcggttt
     5701 gatcatttct ttagttggtg tttggctctc atggacgatc ttaccgattg aaacaatggg
     5761 aaacatggct gaagacggac ttttgcctaa agcatgggga aaactcaaca aaaatggggc
     5821 accaacttat gctatcgtat tgacgacgtt atgtacgaat gtctttttac tttcattatt
     5881 attcactgac agtgcttata actttgctta cacacttggt acagcagcta ttttcttcac
     5941 gtggcttttc ttaggattgt accaaatgaa actttcttat cagcggaaag aatgggtcca
     6001 atttttagta gggcttttag caagtgcctt tcaagtatgg gctatgctag tcgtagcctt
     6061 taatgaagta atgcttgttc ttgttctttt cttgccaggc atttattttt acctcaaagc
     6121 acgtaaggag caaaacgagc ctgtacaaca tatccactta gataagattt tacttatagg
     6181 tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc actgggatgc ttcacgtata
     6241 gaagtataaa agaagtataa agagaggttg tgagcctgtc gtttattcaa aaacatacta
     6301 ggaaaggagc tgtgacacaa atgttgtcac agctcctttt tcagaataaa cggtgtttaa
     6361 gagacagcca atcggcaata aggctgaacg tctctttcac agcctctttt ttgatgagtc
     6421 atcatacatt ttttcgctta aatacgagat atcctaatgc taagaacaaa acgatgtaag
     6481 ctaaattacc gaatagcatt tgattagtag aaagatgagt caattgcttc atgatctctt
     6541 cgtttccaat ttggttttgc agattcaaca tactgatcgg attccatttg atccactccc
     6601 atttttggat gagtgcaata aggataccag atatgattga cgaagcaaaa taaaagacga
     6661 ttcctgctga gatggatgcg ccggaactat tgataaagca agcaatcatc aacactaaac
     6721 ttaaaatcag ccataaaccg atataactac ccaaagtata agtgaacaag ctttggatca
     6781 gtgtttgacc agtagaaacc ttctccgtga aggaaatcga agggaaaaga atcagtttca
     6841 tcaacacagt gacaataata gctaaaaggt ataaataaac agaataaata actaatgtga
     6901 tccatttgct tactaatatt tctcctcgag aatactttcg ataaaggaga tttttcaatg
     6961 taccatattg tgcttccatc gaaatgatcg tgcttgcagc tgcaatcatg ataaagacaa
     7021 tccaagatgt ggcactaaaa agctgagcag tcatttcaac tggatcaatg atcgtcgtgt
     7081 attttttggc tagaagtgct gttcctatga gcaaaacaac taatagaaca gataaaacag
     7141 cagttgactt tttcttgatc aatttaaata attcttgttt tacaagcaat gtcatttttc
     7201 ttcttccccc tttactgaat ccagaatttc caatagagaa gattccaaat cattcgtcag
     7261 atgctggacg tctgtcacat ggatattttg tgcagacaat agctggatca cggttcctaa
     7321 taattcttcg ttttctttta aaagcttgat tttatcagga tcttccaaag tatatccagc
     7381 ttctgtcaag atcgttcgag cagctagatc atttgttgta gagataacga tgaaatcttg
     7441 cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg atctttcctt gattgatcag
     7501 gatcagctca tcgaccattt tttcaagctc gctgagtaag tgactggaga tcaagaaaga
     7561 ggtgccctga gtagccaagt cggtaatgat ttttcgcaca tctcttgttg cttgtggatc
     7621 caaaccgttc atcggctcat ctaaaataac gatagacgga tggttcagta aggcaagagc
     7681 aattcccaat ttttgtttca ttcccaacga gtaggatttc gcttttttgt gaatgtactt
     7741 ttccatcttg agctgattca cgatagtgtc gatagcagat acatcgttgg tttcagagaa
     7801 taacttcaga tgatcatagc ctgttaagaa tggatagata cccgggtatt cgatcaatgc
     7861 tcctacttgt tccaaccctt tatgactaga gggagaaatc tcattttcac caatacagat
     7921 tgttccatct gagtaatgga tcagtcctaa aatagctttc atgatcgttg tttttccagc
     7981 gccattagga cctactaatc caacgatttt gccaggggat aagctaaaac tgacatcgct
     8041 gagggcttgt ttgtgaccaa agttcttgga taattcttgg accgaaagac ttttcaaaaa
     8101 tattcactcc ttttctatag atagtacaag catacacctg atgagtggag aagtcgctag
     8161 gtcattggac ttatttctta ttttttcata aaaaaattga atattaaata aaaaggctta
     8221 ttttttctga tcagtactta ttccaagtgt ctagtggccg ctagtaatct tgatgccaat
     8281 gatactgatg agtagcatcc ccacaaagaa ccaagtggaa agattcagct tatcatgaaa
     8341 caagaccaca ccaattatga ccgaaccaac tgcgccaatc cctgtccaga taggataaga
     8401 gagactcatc ggcaaacttt tgattgcctt tgacaaaaag taaaaactag caatcatgcc
     8461 aatcactgtg taaaaagaat aattgatttt agaaaaacca tcactccatt tcatcatcgt
     8521 tgaccagaat acttccaaaa ttcctgctat gattaattcc atccatgcca tacttacatt
     8581 cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc atcccttcaa agacctaatg
     8641 gaatgatcat cctctttatc ctacccggtg gcagattgta tatctttttc tgtagtatag
     8701 cacacctatc cagatatcgg caatctcata ctttttagta aagtgtacca agcactgacg
     8761 gatatatcgt tagcggaaaa gaagatgaat gctttgcaat ttcatagaaa gaagttgtta
     8821 tttattaatc atttaaaaaa aacagtaact agaccttatt tctatatatt atcgggcaat
     8881 aaattaaaaa aagctttacc ttacaataag taagataatt tttg
//
LOCUS       DARBER010000105         8886 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARBER010000105 DARBER010000000
VERSION     DARBER010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.109"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(354..2735)
                     /locus_tag="SCK06_002213"
     CDS             complement(354..2735)
                     /locus_tag="SCK06_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1152177.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2725..3372)
                     /gene="recU"
                     /locus_tag="SCK06_002214"
     CDS             complement(2725..3372)
                     /gene="recU"
                     /locus_tag="SCK06_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG1152178.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3436..3975
                     /locus_tag="SCK06_002215"
     CDS             3436..3975
                     /locus_tag="SCK06_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG1152179.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4065..4484
                     /gene="gpsB"
                     /locus_tag="SCK06_002216"
     CDS             4065..4484
                     /gene="gpsB"
                     /locus_tag="SCK06_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG1152180.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4506..4873
                     /gene="rnpB"
                     /locus_tag="SCK06_002217"
     ncRNA           4506..4873
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SCK06_002217"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5082..6248
                     /locus_tag="SCK06_002218"
     CDS             5082..6248
                     /locus_tag="SCK06_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG1152181.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6294..7790
                     /locus_tag="SCK06_002219"
     CDS             6294..7790
                     /locus_tag="SCK06_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG1152182.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7871..8554
                     /locus_tag="SCK06_002220"
     CDS             7871..8554
                     /locus_tag="SCK06_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1152183.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2813 a         1598 c         1873 g         2602 t
ORIGIN      
        1 gcatactatc agataactca aaatgaagcc ttctttcaaa aaattattca aaaccatata
       61 ttgtattttt tatttgataa taacaatggg attattaaga agtggcacta aataaaagac
      121 tagtgtgtat ttagccgatt taattataaa caagtaaatg gataaagtat atgtaatgat
      181 aaaagaaaaa tggattttct aatttttaag ctaaatgtta gatcacacat gtatatgctg
      241 gatagtctta taaaaaatca tgtaatacag cttggtacat gatttttatt tgtagctgtt
      301 ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt ggtttctttt ttactatctg
      361 tttccagaag ctgaggtgct gctggatggt gtatggtcat ttgcagcatc cgctccgcta
      421 gttgctggct cactggaaga agcaggagga gcagatgaag acggtgtcga gctttcttcg
      481 gatgaagaag aaggaaccgt tgttgaagac gctggctcgc ttgtttctgc tgtcgtgctt
      541 tcttttgagg actcagcagt tgattcgctt tgactagtag agctagagct ttgagtcgtt
      601 gtctctgttg ttgagcttcc aggagtctgg acatagctgg aagatggaat agttgttgat
      661 ggtgtaattg cattcgatct agctgtatat tgatctttgt agtacagttc accgcccaca
      721 cgaattaatc cgcttggcat ctcccagtct gtatttgtca cgttagctga aacatactgc
      781 ataagctcgc gataaacatc agaggctacg tgtgacgatt ctgaagtgac aggtgtcatt
      841 tttttattgt aacctgtcca cacggaaatc gaataatttg gcgtatagcc cgcaaatagg
      901 atatctggat acaccccaga agaaatacca agttttgcat actcgtcatc tgtgtaattg
      961 gatgttccgg tcttaccggc ttgatataac ccagcgattt gcgcattcgt accagttcct
     1021 tcagtgatcg tatcttttaa aatgtctgtg atcatataag ctgtttctgg agacatggct
     1081 gtttttccat ctggctcata agtttcttct gttccgtctt ggaagacgat tttattaaca
     1141 tattgaggtt tgtaataagt ccctccattg gcgaaagccg cataggctgc tgccattttt
     1201 aaggaagaag ctccgtattt tgtcccatct tgttcttccg tattactaga gatcgcattc
     1261 gattgatgga tcgtactgta ttcgatccca agatttttta agaaagaagc cactttatct
     1321 gaaccgactt cattgaataa tttgactgct ggaacattac gggataagta aagtgcttgg
     1381 cgtaaagtaa tggtgcccat atattgatta tcccagtttc ctacaggagt cgaagtccct
     1441 tcatagttat acggtgcgtc agtaatagtt tttccagtag aatatttcaa atattcaaaa
     1501 gctggtccgt aatctgtgac aggtttcatc gtagaaccaa aatcacggga agtattcact
     1561 gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac gtccgccaat ctgggctttt
     1621 actttcccgg tattggtgtc gataagggtg gaggcaactt gcatctcgtc gtctgggtaa
     1681 gagacatact gatccgtgtt gacgatatca tatagtttct tttgggcgtc cagatccaaa
     1741 ttcgtataaa tctctaaacc gtctgtgtag acattcttat ctgttttttc ttgtacttca
     1801 ttgatgactt ctttgacata attatcgacg atcttagtat tgtcatcgga ttgagttagt
     1861 tcttgcagac catccgttac cggtacatta acagcttgat catattctgt ttgagagatt
     1921 ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc ttttcttcgc ctgatctgga
     1981 taaacataag gatcataggc ggaaggggct tgaggcatcc cagcaagcaa tgctgtttgc
     2041 ggtaaagaaa gttctgataa tttcttacca aagtacatct cagaagctgt ctccatcccg
     2101 tacaagccgt ttgacatata cactttattt acgtagtagg tcaagatctc ttgtttcgat
     2161 tttttttgtt ccaatcttac agccatccac gcttcttggg ccttgcgttt cagagtctga
     2221 tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt gctgtgtcaa agtacttccg
     2281 ccttgcagtc cgccagaggt aaagttagag agggcagaac cgatgatccg aatagggtca
     2341 accccgatgt gtttataaaa acggcgatct tctaccgaca cgatcgcatc ttctaatgtt
     2401 ttcggtaatt cattcgcaga aattttttca cgcttttctg cgcctaaatc ttcgaatagt
     2461 tcgccatctt gtgtatatag tttggaagat acggtagcgt ctaatttttt atccgtcaat
     2521 tcgggcgcat cttttgcgta ataccagaaa agtcctacac ctgctaaaaa gaggatacag
     2581 aaaaatgaga gtattcctaa aaggatcttg ataaataatc cttttttatg tgttccagag
     2641 gactttccag aacctttgcc agaattcttc ttcacactct ttttaggagt aggctgtttc
     2701 tgtcgtctag agcttctagt ttgttcattt gccattcttg ctttctcctt caatagataa
     2761 tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc cgcggagaaa taccagtttt
     2821 gatctctatt cctagttctt cgatcaaaga taaaggtagt gatttgcgtc ctttttcctt
     2881 ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa taaaaacagc gcttcaaaga
     2941 cgaaaaccat aacaagacaa agcagacccc attttgttcg aggcatgctt tcatatgttc
     3001 gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt ttgttcttgg tttcttttgc
     3061 ttcaaagtcc aagtaaaagc ctcgatagac gccattatag tctgtagttg aggcttgggt
     3121 aaagtaggct tccttgatca cagcagcact tctcttagga tagtctactt tcacgatttg
     3181 gataggggta ggctttttat ggataaccgc caacccgcgg gaaagataat atttattact
     3241 ttcattgatt gcttcttcaa agcgcatccc ccgattgccg aattcagtga tctgatttct
     3301 tttttcttga attttttcga cgggagaggc attcttataa taaggttggc catttggata
     3361 gcgcaatacc atctttaatc acactcctag gccacattat agcaaaaaag cggatagaaa
     3421 gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg tgtctgggta tcgaagtttc
     3481 gaactcggtg tttttaaaga aaatgatcct aaaatccaag ttgttaaaaa tgttttaaaa
     3541 aaagaactcc atcagttagc agaagaaggg ctggaatggg tacttgtcag tgggaatttg
     3601 ggcgtcgaac tatgggctgt gcaaatagta gcggaattga aagaagaata tccggaacta
     3661 tctcttggta ttttgtatcc tttttctgat tttggtaata actggaatga agggaaccaa
     3721 gaaaaaaaac aattagcaga acaattagca gattatgtcg aagctgttag ccatcaaccc
     3781 taccaatcac ctagtcagct aaagaatcac actcgtttta tacttgagca tacagtgggc
     3841 tgtctgctga tctatgatga agaattccct ggaaaaacga agtatttttt tgaagatgca
     3901 cagaattacc aagcagatca tccctacgaa attcggctga tcagtatgga cgatcttcaa
     3961 aattttagtg aataatcgat attgtgtttg atttatcaac ttttttccta tacaatgaaa
     4021 aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt aaatatggcg aatttggttt
     4081 atagtcctaa agacatttta caacaagaat tcaaaacaaa aatgcgcggc tatgatcccg
     4141 ttgaagttga tgaattctta gacaatatca tcaaagatta tgaagcttac agcaaagaac
     4201 tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa agtagctcaa ttatctaaaa
     4261 ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc accaaagagc gcagcagtga
     4321 ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg tgaagtattt ggtaaaaagc
     4381 ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa tccaaataac tacacaaatg
     4441 cagacacaag cttagatgat aatgaaaaaa cacgtcaatt ttaatttttg attaatgaat
     4501 ttgcggtttt cgggtaatcg cggtctgact tgatcaggct gaggaaagtc catgctcgca
     4561 caggctgtga tgcctgtagt gttcgtgctt agcgaaacca taagctaagg tactctttgg
     4621 agtaacggca ggaaaacaag ctaaggcttc ggctatgctt aagtatcctt gaaagtgcca
     4681 cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc ggtaaacccc tcgagcgagc
     4741 aacccaaaca atggtagggg cgctttttgt ctggaagaag aacaggacaa aaaggcagta
     4801 taactgcaga tagatggtta ccgctttgtt ttttctccct ggaaaagcaa agtacagaac
     4861 atggcttata gaaaaccgca aacatcaggg aattaggtcg tgacaaaagt aatgcgttat
     4921 aagaaacaag aaaaacattt gaaagctatt tttcaatact tcagatgaat cgttcttatt
     4981 acaaaacaaa cttttgtatc accggttatt cggaatttga gtaaaagtgt ggggtaggac
     5041 tgatgtccca cctccttttt ttataaataa ggagattgtg tatggaaaaa aacaggaagt
     5101 tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact ggttggtaaa gaattaagag
     5161 atcttggaat cgaatgccaa gttgagaacg gacgtgcacg ttttgaagga acaatggaaa
     5221 cgatcgctac agctaattta tggttacgta cagcagatcg agtaaaaatc gtggttgggg
     5281 aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt aaaagcattg ccttgggaag
     5341 attttcttcc actagatgca gcttttcctg tagctggaaa atcgatcaag tcgaaactat
     5401 atagcacacc tgattgccaa gcgatcacaa aaaaagcaat cgttgaacgt ctccgtacct
     5461 attatcaccg tccagcttct gtaccactga cggaaactgg cgcacatttt cagttggaag
     5521 tagctttgct gaaagaccat gtgatggtaa ctctggatac gacaggacca agtttattca
     5581 aacgtggtta tcgcttagaa aaaggtggag caccattgaa agaaaacatg gcagcagcac
     5641 ttattatgtt gacaaattgg cgaaaagacc gtccatttta tgatcctgta tgtggttcag
     5701 gtacgctttg tattgaagca gccttaatag gccataatat cgcaccagga ttcaatcgtg
     5761 agtttgcttg cgaatcttgg gattggttca gtcaagaaat catggaaaat gttcgatcag
     5821 cagccgaaga aaaagcagat tatgatattg aattagatat tactggttca gatatcaatg
     5881 gacggatgat tgaaattgcc aaagccaatg cagaagaaat cggattaggc tcttcgatta
     5941 cctttaagca acaagcagtc aaagacttca aaacggataa agaatacggg gtcatcgtgg
     6001 ctaatccgcc ttacggagaa cgtctaggag aagaagaaac tgttcggaaa ttatataaag
     6061 aaatgggcga tgtattccgt ccattaaaaa catggagtaa atatatcttg actagcgact
     6121 tagcgtttga ggaattttac ggacaaaaag caaccaaaaa aagaaaattg tacaatggtg
     6181 cattgcgtac ggatctattc cagtattggg gaactcgtcc gcctagaaaa aaagagaaag
     6241 acgaataatc ttactaattg attcattcaa ttggatgttt ggaggaatag ggaatgaaag
     6301 aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct acaacaagct ttaggaatcc
     6361 tcgattggga tacacaaaca ggtatgcctg aaaaagctag tgcataccga agtgaagtag
     6421 acagctatct ttacagcatt tatttttcaa aaaaaatcgg tccaaaaatc aaagaagcca
     6481 ttcgctattt ttctgaacat ccggaagaat tgtcggaagt tggaactgcc gtttttgcca
     6541 aagtaaaaga agagtatgat ttggaacatc gcgtaccgga agatcttatg agaaaatata
     6601 cagctgccgt ttcttcagca cacggacaat ggcaaaaagc tagagaaaca caacaatttt
     6661 ctgattttca acacgcattg aatcaaaata ttgaattaac aaaacagttg ataccttact
     6721 ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa tcaatacgaa cccgggatga
     6781 ctgtcgaaat ccttgataag gtatttgatc agcttaaaga aggaattctt tcgatccgcc
     6841 gtacgctcca agaaaaaggg actgaacctg aaacagactt tttaaatcgg agaatgacga
     6901 aaaaacaaca gaagagattt gtagtgaaag taatcgaaca acttggttat gattttagtc
     6961 gtggtcgttt agatgacact gtccacccat ttatgactgg aattaatccg aatgatgcac
     7021 gaatcacgac acgatggaac gaaacagatt tcaaaatggc tgtttttgga gtgatccacg
     7081 aggctggtca tggtatgtat gaacaagaca tcgatgataa atatgcgtat acaccggttt
     7141 atgaaggtac atcaatgggt atccatgaat cacaatcgtt gtttaacgag atcattattg
     7201 gaagtagtcg cagcttctgg aaaaagcaat atcctttctt tcaagaatgt gcagaaggaa
     7261 cattcgatga tattcctttt gacaaatttt atcgttcatt gaaccataca aaatcaagtt
     7321 tggttcgtat cgaggcagac agtttgactt accctctcca tatcatcatt cgttatgaaa
     7381 tcgaaaaaat gattttcaat gaaggggtag atgttgctga actacctgaa atatggaatc
     7441 aaaaatatga agagtatctg ggaatacgac caacaaatga tgtggaaggc atcctacaag
     7501 acgttcattg gtcaggtgca agctttggtt attttccctc ttatgcatta gggtacatgt
     7561 atgcggcaca attacttcat tctttgcagc aagaaataga tgtggaaaat gttctagcgt
     7621 cagacgatta ttctccaatc aaaaactgga tgtcagagca tatccatcaa tatggtgcat
     7681 ctagaaaacc aaatcaatta atcatggatg caacacatga accgcttaat ccgcaatacc
     7741 tgatcgacta tatgaaagac ctgtatttta atgtatatca ggtaaaataa aatacaattt
     7801 acaaagatag ctgaaattca tctaatctat agatgttttc agctatttac ttaagaaaga
     7861 agtggaacga atggataaat tacaagtgat cgctgattat agtatggacc aattagcgca
     7921 tgatcaaaca ggacatggga gcgatcatac caaaagagtt gtcaagttag ctgaaaaaat
     7981 tttggagacg gaaccgcagg cagaccgctt tgtgacgctt gcagcagctt acctacatga
     8041 tacgattgat gataaagtcg ttaaaaatga gaacgaggca aagcaacagc ttcgtgtatt
     8101 ccttcgctct ttgccaatag tagaggaaca gatctcgatg attttttcca ttatcgaaaa
     8161 tatgtccttt tctaaaaatc tctctgaagc ggccgaacta tctctggaag gtaagatcgt
     8221 tcaagacgct gatcgtatcg aagcattagg agcaatcggc attttgcgta ctgcttattt
     8281 cggcggtgga catgggcacc cgatttttga tcctgaactg tatccgcaaa cattcaagga
     8341 caaagaaaat taccgaaaag gcacgacagt gatcaatcat ttttatgaaa aactgttctt
     8401 gctgtctgat aaaatgaata ctgattacgg atatgaagaa gccaaacgaa gagaaacctt
     8461 catgcgtgaa ttcttagaag aattttttct ggaatggtct attgacgatg aaaattttac
     8521 tccagacaat tggatcaaaa ataacaaaaa atgatggagg ttgtgacaga agtggtcagc
     8581 tccaagatat ttcggatcat tttccgtaaa agttacttct ggaacactgt ttaccaagag
     8641 gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa tttcaaaaaa tagcttctcg
     8701 tgtttgatga atattgtaac ttgtgattcg gaattggcag gagaacaccg tttattcgga
     8761 agaataggac tgtgacaaga cttttgtcac agtccttttt tcgaattcaa caagtaaaac
     8821 ttttaagaaa tattcagaga gtaaaattaa aaattgctga taaacgaaga tggaaatttt
     8881 atgtat
//
LOCUS       DARBER010000106         8645 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DARBER010000106 DARBER010000000
VERSION     DARBER010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.110"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..135
                     /locus_tag="SCK06_002221"
     CDS             <1..135
                     /locus_tag="SCK06_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1152184.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(88..324)
                     /locus_tag="SCK06_002222"
     CDS             complement(88..324)
                     /locus_tag="SCK06_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1152185.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFLMILFVKLSETKLSIYVTQKNF"
     gene            complement(744..1865)
                     /locus_tag="SCK06_002223"
     CDS             complement(744..1865)
                     /locus_tag="SCK06_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG1152186.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2193..3602
                     /gene="istA"
                     /locus_tag="SCK06_002224"
     CDS             2193..3602
                     /gene="istA"
                     /locus_tag="SCK06_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG1152187.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3599..4327
                     /gene="istB"
                     /locus_tag="SCK06_002225"
     CDS             3599..4327
                     /gene="istB"
                     /locus_tag="SCK06_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG1152188.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4455..5366)
                     /locus_tag="SCK06_002226"
     CDS             complement(4455..5366)
                     /locus_tag="SCK06_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1152189.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5383..6390)
                     /locus_tag="SCK06_002227"
     CDS             complement(5383..6390)
                     /locus_tag="SCK06_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1152190.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6387..8516)
                     /locus_tag="SCK06_002228"
     CDS             complement(6387..8516)
                     /locus_tag="SCK06_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1152191.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8521..>8645)
                     /locus_tag="SCK06_002229"
     CDS             complement(8521..>8645)
                     /locus_tag="SCK06_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1152192.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2654 a         1759 c         1505 g         2727 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat cattaaaaag ttagtaaata attgccctct
      181 aagttccacg gtctgttcat taataacttt atatcctcgt ttttcaaaaa atggctttgc
      241 ggtaatggaa gcgtgggtag tgattttttg cataccagtt tctaaaacat ggttttccaa
      301 ttcatctgca attttagtcg caataccttt tccttgatgg ctatgttttt gtcacaactt
      361 cagtattcct gtaacaatac ggatttcagt atacaacaac agcttttgaa aagatacttg
      421 caaaaggatt aagatatcta tgacctgcta tgctcactga tgttgaaaag aatgttttat
      481 taggattgag gtaagcaaga tagtgaatat gtccggaaag cctgtaaaat aaggcttttc
      541 ggacatatgt gaagttataa aaagatagcg aaaaatctat ttagtatcac taatgttgcg
      601 aacatatcgt cagaatagtc tgacatttat ttcttgctaa aaatttcaaa aaagtcaagt
      661 gctgacgcag cggatgacta ttttccagaa aattttagta tgtattcaaa aatcaaacgt
      721 gacaacagct attaactaca tcctcatcca ggtctgtaac gtttgaacca ctcttctgcg
      781 ggctcaaaca ttaagtaatg gaaggtacgt ttcaattgaa acagcgtcgc tgtcgtattc
      841 aactcaattt ttatcacata agtgagaagc gttgcgatta aggtgaggat gacttgattt
      901 atcgcccctt gttcactatg tgaatatagt cgtttaatgg tcagattttg ttttaagtgt
      961 ttgaacaaca attcaatctg ccaacgggct tgatacatgt cggccacttc gtttggcgat
     1021 acatcaaaga gattggtcac cattcgcagg attttttgtc ccttggtaag aaccgttacc
     1081 aacctaaaac gctctgtcac atggttgaca ccgccgacaa gaatcacttg ttggtcactg
     1141 attatcgtcc catcacgggt tgttgtatct gctacttcaa tttgatccaa gatggtaatt
     1201 tttgtattag attttgtccg tgtaacgaag aaatagccgt cacgatttag tttgtctaac
     1261 aacttgtaat caaagtagcc gcgatccacc acatacgttg cttgcgtttt atcaacaaga
     1321 acttcaaggt gattacggtc atgctcactc gcatttgtca tcgtaaaact ttcaggatac
     1381 tgattggttt tatcaagata acagagattc aaatgtagct tgatgccaga ctttgttttc
     1441 cgaaacttgg cccatggaaa ccatgctttg ttcaacggaa tagtcgttga atcaatcaat
     1501 tgtagactgg ttgtttttga gcttggctgc tgagcagaaa ttctggcgac taattgcgta
     1561 aaaatttcca tcaatacttc ttgcttgatt tctggcgctc tacgagataa cgagctgtaa
     1621 cacagggaat caatgccaag ttccttgcag aggcgctgat ctaaaaaggc gcgatcaatc
     1681 tcgcgatagc ttggcaattc ttcataaacc gcatgaagta acactttaag cgtcgtttgg
     1741 aagtctagtt ttttactgta gaaatcaaag tcgctaatta agctttgaga gttttctgat
     1801 aaaccagaaa aatttattgc agaaaaccat ttttgaaatg aggtttttac gttatactta
     1861 tccatgcgtg agtccttttt attggtcttg gataagtcat ccgagaatag tataaaggac
     1921 ttttttgcgt ttggatagct ttgtattagg cttttgatgg gttaaaaagt ctttgcaaca
     1981 ctagtaattt agtatttatg tataaattga aagatatttt accaccactt agtcttctta
     2041 aaagtgatcc aacattttgt ttgtggcaga atttaactac acactaatgc agccgaaagt
     2101 acacaactat gcagcagttt gactgcgaac tggtttcctt ccatcataag tttgaatgac
     2161 ttagtgatgg aggtacagaa aggttgttaa ctttggatga tattaaaaca ataaaacatt
     2221 tgagaaataa tcgtgatcag tcaattgatc agataagaaa agcaatgaat atcaattggc
     2281 gaacagcgaa aaaatacgcc gatagtgatc aactattcat tacagatccc actcgaaaaa
     2341 acaaatccat gatggatgat ggttggggag aaatcatcgg tagttggcta gaagaagacc
     2401 tacgtttacg cagaaaactt cgtagaacgg ccaaagctat ccatgaacaa ttgaaacctt
     2461 tgggtttccc tggctcctat cgaacagttt gtgtatacat ttctcaatgg aaaaagcagc
     2521 gttacgacga aacgaattac caagctgtcg atcgtcttga tcatccgatc ggagaagcac
     2581 aggtcgattt tggaacaatg gaagttatgc atgaagatca catgaaagat atacaacttc
     2641 ttatcatgtc atttccctat agtaatgcga cgtccgcaag tgccatgcca gctcaaaacc
     2701 aagagtgttt cctggaagca ttaaaacaac tatttaatca atgcggcggc gttccaaaaa
     2761 cgatcagaat agataactta actccagcgg tgaaaaaaac aaaatcaaaa tttgaagaag
     2821 cacaactaac cgatgaattc ttacgtttca ccattcatta tggttgtgat gttcaagtct
     2881 gtaacccacg tagtgggcat gaaaaaggac atgtcgaaaa taaagtgggt tatgtccgat
     2941 acaatttctt ctcgacttcg ccaatcatgg actcttttga gtccctaaat aagcaacttg
     3001 aagaacaatt aaaagcagat cgaaaaagga cgcattattc aaaacatcaa ttaatcgaag
     3061 atttgtggaa gaaagaaaat aaagcgttac attctcttcc acaagaggat tacccaattt
     3121 ttaaagaagt ggaagctaaa ctaaatcgtt atcacgaaat gaaattggat caaacggtta
     3181 ttcatattcc taaagcatat ggctattctc aattgactgt tgttttgact tggaataaat
     3241 tcaaagttct tacgcctcat ggagaaatat taatgaacga atatcgcccc tatatgaaca
     3301 aaaaaagatt gatcccatgg agtgacatca tgaaacaatg gagtaataaa ttgaatgtcg
     3361 ttagttattc gaggtattgg aagtacttgc ctgcacgcct tcaaaattat ctagcgattg
     3421 ataataatca cctgaaatac agacggataa aacaattact aagtttatta gcgacctacg
     3481 atatccaaca aattaatgaa caattttacg aattagtcgt tcaagaagac gccccctctg
     3541 actttgatgt gaattggtca aactatgatg cattaacaaa aacaggaggt gtgcaaaaat
     3601 gattggacag acgttagaag attattgtaa aaagcttcga ttaagccata tcatcacgaa
     3661 cataccaaga gcttctttcg aagctccaga acaatttatt accgatttat tccgtttaga
     3721 actggagacc agacagcaga tcaaattaga acggatggaa aaattagcca aatttcctgt
     3781 accaaaatcg ctaaagaact atgaatggca cgaagatatt tttctaccat ctcatctaga
     3841 taaggagacc cttgtctctc ttgattttct aaaaaggcaa gaaaacattg tttgtgttgg
     3901 ctctcctggt acagggaaaa cacatctagc gatcgcatta gggagaaaag cctgtagtga
     3961 aggtgtttca accaaatttt tccgagttaa caatctcgta gagcaattag agaatgcatt
     4021 aaaaaaacaa cagctagcca gtttcaagaa gcgattcgaa acaacagaat taattattct
     4081 tgatgaaatg gggtacttac cattgacaaa agaaggagct gaacttctct tccaactgat
     4141 ttcagattgg tatgagcaaa agagcattat tattacctct aatttagaat tcagtcaatg
     4201 gaatcgagtg tttatggatt ctcgattaac agctgctctc gttgatcggc taatccatca
     4261 cgctcacatt atcagcttca aaggcgaaag tttcagattg aaaaattcat tatctaaatc
     4321 atattgaagg aaaaaactag ttgcagcaaa actctgcact ttcggctgca tttttttgta
     4381 aaaagctatt gcacaacaca aacatttgaa cgacgtaatt ccaaatgttg gattgctttt
     4441 agataatctt ttatttattc acaattgcta atagtccaat tttcattttt gtatagtttt
     4501 aatctatact gaaaaacact tgttgttttg gacaactcat caagatactc aacaccaaca
     4561 atcacttgaa tcgctttacc cttttgtttg taaataggat tcactaattc cacaaatttc
     4621 aaattagtat taattggttg catcgcgtct ttttcaacat aatgctctag ttctttttct
     4681 gaggctgctg gataaagcct gaaaaacgtc tctaaaaact cggtgatttc ttccgttgtc
     4741 tttgaatcaa tttttgaatc actttttact tgtcttactt tatgttttgc cttttctggc
     4801 ttaccaatca tagtgggtaa actcgttaca atatgattgc ctttttcatc ttcaaagatc
     4861 atcagacgat atgcagaaga aattgattgt gtctttttcc cttctgtaat cttttgatct
     4921 accacaaatg aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttactgat
     4981 acaacctcgg aactagtggg aatatccgct cgaaccatat cttggctcaa agcgagccct
     5041 tcttctgtaa ggtactgctc aagattagtc attctctttt caattacctc cttgttattc
     5101 ttccatgaaa aatattcttt cacaaaatct acagtaaaat tttctactcc agaggtattc
     5161 actattttct ctttcacgac cactttctca tgagttgtat gctgatcaat ggccgtgaag
     5221 tttttgtaga ttccaaaagc aaggctacaa gacaataaaa gccaaagggc aagcaccatt
     5281 tttcgatgct tgcccacgga tactactcgt tctttcttct tttttggaat cttttccttg
     5341 ttacttcgct caatttttat cttcattttc cattcctctt ttctattgtt tataccgacc
     5401 agcaccaata atgtgctgtt gccaatagct atcggtaaga ttggtccaac ccaccggatc
     5461 tcctgcatga aacattctgt tctctcctgc ataaatgccc acatgagtca cataagtacc
     5521 ggcatcataa gttgaatgaa agaaaatcaa atcgcctggt ttggcatctt ttaagtcaat
     5581 atgagtcata gcatcatatt gatcctgagc cacccgaggg agtttcaaac caactttggc
     5641 ataacaccat tgagttaaac cactacaatc aaacccagtt ccgggacttg acccaccaaa
     5701 tacataagcc gttccttcat acttgaacgc ctcgtcaaaa attccttgga ctgttttatc
     5761 atcaaactgt gttgtagtta ggtactgctt cactaataag caataaaaca tattgccata
     5821 gttataccgc cacccactat tttccttaat cgcgatggga ttgggataat cgactttttg
     5881 accacccgat ttttcttttg aaaaagattc ggctaaagca aaactatgct tctttccgtt
     5941 tttcgcgaca taatccaaat acccgtcacc atagttataa gcctgaatga tactatcttg
     6001 atcgcatcct agttctttgg aacacttaac caaagacgaa aaatagagac acacttgttt
     6061 gatggatgcc tcggtactta atgaattcgg tgataaacct gctgattcag aactttgcat
     6121 gacatcttcc attgtacctc cagattcgac ttgaataatc gccaaaagaa tcgaaacata
     6181 ctctgttata tctgattctt tggcatactt ttccaccatt gaacggtgtt tcaaaactac
     6241 ttctgagaca ctactacctc caggagaagt tccagaacta ccacttccct caccgtcctc
     6301 accaaacaac aaacccacac acatcaagat gcccacaaaa gagatggtca ggaaggttaa
     6361 cgaaataata ctcaattttt ttaagttcat cttttctcac ctggctttgg ttttgggcga
     6421 gtgttctttt ttctttgtac ttgttgattg gttcgctttg gaaaagtgaa tctctgtttc
     6481 acttgttgaa aggaattgtt cttgcttagt ggctgagtgc ttccgtttgg aatgacttct
     6541 tgactagaaa gtttccgttt gatttcagga ttcgaatgta cttgtaccct tggttttaaa
     6601 gacctatttt ctggtagttc atttttctgt ggttttatct ccttttttat cggaacattt
     6661 tcccttcctt tctcatgacg attccatgct tcgttcaata ctttgcgttt ttcatccatt
     6721 tttgcgtctc ttcgttgtcg ttgtttttct cgcaactccc ctcgatttgc tttttcttcg
     6781 aaaggccctc gcttaaagtc ctctggtact tttttcgtct tttccatgcc tttatgaagg
     6841 ctgtatttca agttagttgg tgcatcggtt acttgatttt ttgcttgttt cgctttgtcc
     6901 ttcacgattt cttttgtatc gagtactttt ccaacttttc taccaactaa atttattcgt
     6961 gattctttct tcgtgcccac tggttgttct tgttttttca gatcagaatt tagctcgtta
     7021 atgcttctcg cggaatggtt ctttctattg gacactctat tgatttttga actacctttc
     7081 ccttttgtag aactggtagc tagtttccca ccaactgctg caccgccacc aaaagcaaaa
     7141 gttcgaccca catttcgatt aagctgtcgc aattttcgat tcatcaacat ttgcggtttc
     7201 cgcatgactc gccttcccat tgactgacta tcattacttt gaaggttaaa catgctcata
     7261 atgtcaccta atttgaaata gataccggca aaggtcacaa tttgtagaaa agctaccata
     7321 aagaagggat aggtggccga aatattgaaa aacatcgtgg agatactgaa tgcagtcgta
     7381 atgactaagg taactcccgc tcgcatcata atcgtattga acaaacgaat aatcgccctt
     7441 ttgcccaaac tctcatacgt cggcaacatc gacaataaga aactaatggg cagaaacatc
     7501 gcaaagataa tgaaaaggat ttgtgagaac aacataatcc ctgtcagaag aaacacaaaa
     7561 aaggaaatcc caatattaaa caacactaaa aagaccacgg ttcctaaacg gttcatggtc
     7621 ttggtaatac tgagattcgc attgtcatta tcttcaatct ctgctttgac tgcttcttcc
     7681 cgatccttac ccttattttc atccggactc acggataaaa tcttattaac ccgaccctcg
     7741 ccaatttctt ctatattgga atcatcgaat tgtaatagta accagggttg ttgaacttga
     7801 atcgaaaata aactgtctcg aatcaaatcc acactatctt ttccctggct ttctgaatta
     7861 ggtacaacaa tcttggttcc aaggctaaga gccgcttcac taacgtctga gctaaagtca
     7921 ttgatttttg taatataggt gggcgcatac gcaatgaaag aacccgagag tagaaaaatc
     7981 atcacaaaat taatgacggt tcggactgct ttggtagttt ctcgttttaa taatccggta
     8041 tacgccacat aaactcctag agctaaaatc atcaataata ggaacccaaa gtagaatccc
     8101 gaagtctgta atccattttc agtgataccg gctaaagttt ggatattctt tccaatactt
     8161 ttagccgtat ccgaaatgaa atccaacttg taagcttctt gaaccacata gcccgtggcg
     8221 tttgataaat acagacttac tgtccaaata aaattggtaa tggcataaag accatacatc
     8281 acgcttttcc ctaagccatc cccccaattc caaggtaacc aatcccaaga actatccaca
     8341 aagaaatcca gttgataatt gttcagagaa tatttggaat aaaggtttcc ggcttcaact
     8401 gtatcatcaa ccagacctgt cgcctcagct accgtaccga ctaaactcaa tagaactgtc
     8461 agagtgacga tgctaataaa aacgattccc acaatccgaa aaagtttctt tttcatcccc
     8521 tcactccatt ctctcttgaa ccggcggacg agtgtcaaaa gcatggaaga gttcttcaaa
     8581 gactggatga acttgaacca cacctactcg cccatacaag tcttgcatca agcattgacc
     8641 gtttt
//
LOCUS       DARBER010000107         8619 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARBER010000107 DARBER010000000
VERSION     DARBER010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.111"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            26..535
                     /locus_tag="SCK06_002230"
     CDS             26..535
                     /locus_tag="SCK06_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1152193.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            628..1790
                     /locus_tag="SCK06_002231"
     CDS             join(628..904,904..1790)
                     /locus_tag="SCK06_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1152194.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2070..3050
                     /locus_tag="SCK06_002232"
     CDS             2070..3050
                     /locus_tag="SCK06_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1152195.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3172..4590
                     /locus_tag="SCK06_002233"
     CDS             3172..4590
                     /locus_tag="SCK06_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG1152196.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4661..6130
                     /locus_tag="SCK06_002234"
     CDS             4661..6130
                     /locus_tag="SCK06_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1152197.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6140..6607
                     /locus_tag="SCK06_002235"
     CDS             6140..6607
                     /locus_tag="SCK06_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1152198.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6643..7515
                     /locus_tag="SCK06_002236"
     CDS             6643..7515
                     /locus_tag="SCK06_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1152199.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7596..>8619
                     /locus_tag="SCK06_002237"
     CDS             7596..>8619
                     /locus_tag="SCK06_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1152200.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISID"
BASE COUNT         2968 a         1291 c         1755 g         2605 t
ORIGIN      
        1 ttttattgtt tagggctata ctatattggt acaaggaata gaaagaggtg tttatgtggt
       61 tgataaaatt cagtttaatc aagttataga agaattcatc aaagtaaata atttaatgac
      121 ccatattcaa aaacgaccaa tcagaataac taatgatcta aaactcagta cgagtatgat
      181 tcatttgatt gatgtgattg gaaattatcc tgaaactagt attacggaat tagctgatcg
      241 attaggcgtt accaaaggag ctgtatccca acagattccc actttagaga aaatgaatct
      301 tataagcatt tctcaaaaaa aaggaaataa gaaaactaaa cttgtatcgt taacaaaata
      361 tggtcgaaag gtgtacgaat cacataatta tttacatgaa gagctttata gctcaataaa
      421 tgaagcgtta gaatcattct caccagacca aatttcaacc attaaacaga ttctagaaaa
      481 agtatcttat acaatcaatg aatatcaagt aagtctgaca agaaaggaag attgacatga
      541 aaaaaatgat ttttgaatcg gctacactag actagacaga aaaaataagg tgtgtaaaat
      601 aaatgaaaac acactggagg aatttttatg tcaagacgtc aacgtagaac ctattcaaaa
      661 gaattcaaac aacaagtcgt cgatctttat ctcgcaggaa agccccgagc tgaaatcatt
      721 cgagattatg agctgactac ctcggccttt gataagtggg tcaagcaagc gcaaactact
      781 ggctcgtttc aggaaaaaga taacttgatc cctgaacaag tagaactaat tgcattacga
      841 aagaaaaata aacagcttga aatggagaat gacattttaa agcaagcggc gctgatattg
      901 ggacgaaaag acaagtaatt gataccaata agcacaaata ctcaatatca gcgatgtgta
      961 aagtgttaaa catctcttgc caaacctatt attatcaagc caaaccagtt gaagatgaat
     1021 cagaactgga agaactcgtt caagaagaat ttattcgcaa tcgaaaagct tatggtacca
     1081 ggaagctgaa gcagtgcttg gccaatctag gtcttcaagt gagtcaacgc agaatcggcc
     1141 gtatgatgaa acagcgtggc ttgcgttcaa catacacatt ggctcatttc aaattccatt
     1201 cttctacttg taacgaagcc aagaccgcca atattctaga cggaaccttc ctacaggaaa
     1261 agtcattaga agcgattgta accgacctta cctatgttcg tgttgggaag aaatggcagt
     1321 atatctgcct gattcttgat ttgtttaatc gagaaatcat cggctattca tgcggggaga
     1381 agaaagatgc gacgttagtc aaggaagctt ttgcaaagat tccgtactcg ttgaataatg
     1441 tacaactttt tcacacggat cgtggaaaag aattcgataa ccagacgatt gacgaaattt
     1501 tatgggcctt taatatcacg cgttccttaa gcaaaaaagg ctgcccctat gataacgccg
     1561 ttgcagaatc cacctataaa tctgtcaaag tagaattcgt tcatcaatac cgctttgaga
     1621 cattggctaa tttgcgatta gagctatttg attacgtcca ttggtggaat accatccggc
     1681 tgcacggcac gttaggctat gagacaccga tcagttttcg acagcagaga ttggcgcagc
     1741 gaatccttga taatgagctc ggatgtgata ccggtggcga ggcaccttaa ttatttgaat
     1801 gagcttctgc cttagagaat cagattcgag gaggctcgtt gtcaagggca gtcggaacac
     1861 cagcagcaag cttacgcaat accttataat ttttgtcaaa aaaagtgttg ccattccaca
     1921 tatatcttta aacagctatt gccagataaa aataaatgat attatatata taaatactta
     1981 tccgaaaagg ggaatgagag agtcaaggtt cttggaatgt cgaatagtcg agtaggtaga
     2041 ttttctatct acaaccttgt tgtttaaaaa tgaaaaaaat tggaattaga gaagttgcga
     2101 aagaggcaaa tgtttctcct acgactgtgt cgagggtatt gaatgatcgt ggatatttga
     2161 gtgaggaaac aaaaagaaaa gtttttgatg ccatgaaaaa attagaatac tatcctaatg
     2221 aactggcgag agcacttttt aaaaacgaaa catataccgt aggtttgatt tttcctacaa
     2281 ttactaatcc atttcacgct gagttaattc aagatatcga gttcgagctt tccagcaggg
     2341 gatataaagt gttgctgtgc aatagcttaa acaatccaac aaaagaaaaa gagtacttga
     2401 cgatgttgag aaagaatcag gttgatggaa ttatagtagg aacgcacaat aaagatattg
     2461 cggattatga tattccaggg ctacctattg tagcgattga tagaagtctt ggtgaaaata
     2521 cagttacagt atcttgtgat aattacaggg gtggggaatt agctactcaa ctacttgtaa
     2581 atcatggctg tcagaatata ctgtgtattc ggggagatag taagataaag ctacccgcga
     2641 atagtaggac tttagcatat aaagatgttt tgaataaatt catgctggaa ccgttagtcc
     2701 tagaagtacc atttgtaaaa gaaatcaatg aaaaaaatga aataattaag acatatctgg
     2761 aaactcatcc aaatattgac ggggtttttg caggagatga tttattggct tctttggcga
     2821 ttaacaactt aggaagttcg ggcaaaagag ttccaaaaga ggttaaagtg attggatttg
     2881 atggagctca acaaacattg atttataatc cagaattatc aactattcaa cagcctatag
     2941 atcaaatatc taaagtagca gtaaaaaaat tattgaatat gattaagggc gaaaaggaga
     3001 cggatgaact ttcacttcca gtaaagctca taaaaaaaaa tacagcataa gttaaatcgg
     3061 cgaaaaattc cttcgtcgat ttaaaaatat ttttatgaaa cgggttgaca tatgaaacgg
     3121 gttgacatat attagttgtg aagataaaaa ccttttcact ggaggaagaa aatggagaaa
     3181 ttattaatta aaagcaataa tcatcaactt tactaccaac ctaaggatgt ttgggtcgga
     3241 gatgttatgc cttacgggaa agaaggaaaa ttctttattt atcatcaacg tgatactaga
     3301 aatccagttc cttttggaga accgtttggc tgggctttag caactacgaa agattttgtt
     3361 cattttgaag actttgggga atctctttta agaggggatg acaatgagag agaccagttt
     3421 atctacgctg gttctgtatt tgaggcaaat ggaaaggttc acgcatttta tacagggtat
     3481 aatcgtgaat tcttagcgca gggaaagaca tctcaagtgt tgcttcatgc tgagagtgat
     3541 gattttgttc attgggaaaa atcagaaaca gcattggagt tagaaccaca agaagggtat
     3601 gatattcgaa actggagaga tccatttgtc gtttgggatg atgaaaaaga agaatatttg
     3661 ctaattttag gggcgcgaaa gggtcaggat aaacatctac aaaccggacg tttagtggaa
     3721 tttacatcaa aagatttaac taattgggaa ttcaaaggtg atttttgggc tccgggattg
     3781 tataccatgt ttgaaatgcc agaacttttc aagattggtg actggtggta tcttgtctat
     3841 tcagaatact cagatggaaa taaaatactc tatcgaatga gtcaaagttt ggaaggtcct
     3901 tggattaagc ctaaagatga agcttttgat ggacgggctt attatgcagg tagaacagct
     3961 tatgatggaa aacgtcgagt cctatttgga tgggtaccaa caaaagatca agaaaatgat
     4021 aaaaacaatt atttatgggg aggtactttg gttcctcatg aagtctatca aaaagaggat
     4081 ttttcacttg gggtaaaacc catagacgaa gtgtggaatg cattcataaa tttacatgat
     4141 attgccgatg tagagataga aacagcagac acaagagaag aagtaacgct tgtcaaaaat
     4201 agtggaactt tgtttaattt agaaacaaca atttcatttg atgagaatct tagtgacttt
     4261 tcactgagat tatataaaaa ccttgagaca gatgaatctt acgaatatcg cttcaattta
     4321 gatgaggggc gtcttacatt tgataaaaac ccttgttacc cttggtttca atatatggat
     4381 aaaggactta ttagacctat atctttaaag cctaaccaat cttataaact aaaagtgatt
     4441 gttgatgaca atataatgac actttatatt gatggaatcg ctcttaacgc tcgtgtacat
     4501 ggttgttttg gtcaagcaat tactattaca gcaacaaatg gatcaatgac tgcaataaat
     4561 accaagtttt caaatgaaat aaagaaataa taaacgtcaa tcatgtggca ggtctgaaaa
     4621 tcaaacatga cctgtcacat ttaaaaggga ggataaatga atggactaca aaaaaattgc
     4681 cgaagatgta ttggaaaacg ttggaggaaa agagaatatc tcaagcgcta cacactgcat
     4741 tactcgttta cgtttaattc ttaaagacaa aagcaaagcg aatatggaaa tgcttgagga
     4801 aatagaaggt gcaaaaggtg ttcttttcaa tagtggacag ttacaagtaa ttttcggaac
     4861 aggtataatt gaaaaggttt acaaagagtt cgttgaattg acaggagcta aggaagtatc
     4921 cgtttctgaa atgaaagacg aaggagtcaa taattcagga aatagacttc aacgagcttt
     4981 taaagttttc tcggatatct ttattccgat cattccagct tttattgggg cagcaatggt
     5041 tttaggaatt cgttcgttga ttaccactcc tggaatgttt ggaatggatg gctcattcgc
     5101 tgatcacaat gtattcctaa atgatttatc caaatttctg ggtattattg caaccacatt
     5161 tgattattta ccagttttag ttatgtattc tgcaacaaaa cgttttggcg gaaaccctat
     5221 tttaggatta cttgtaggat ttgttatgat tcatccagga ctagccaatc ggaatgattt
     5281 cgtcatggga actataaagc ctgattattg gcatttattt ggattaagca ttccagcagt
     5341 tgcgtttcaa gggggtgttt ttccagcaat tttgacttct tggtttttag caaaagttga
     5401 gaaaattaca aataaatatt ctcctcaagt acttgcttac attttagtcc caactgtgac
     5461 aattgttctt gccaactttg cactattttt agtgtttggt ccattgggga attttatcgg
     5521 taatgtttta ggtggaatca ttgatttcct atatatgaag atgggtgcct ttggtgcttt
     5581 tgtttttgca ggcgcgttac aaccattagt tgtaacagga acacaacatg ctattcaagg
     5641 tattgaggca aacttagtcg caacaactgg attcaattat atccaaccta tttggtcagt
     5701 ttcaattatt gcccaaggtg gcggttgtat tggtatgtat ttactcttta agaaaaaatc
     5761 taaagataga gatattgcga tttcaagctt tattccaaca ctggtaggta ttactgaacc
     5821 ggctatcttt gctgttaatt tgaaatattc aatcgttcct tttgtttgct cgtttattgg
     5881 tgctggtttt ggtggagcat ttatgaaaat ttttgatgta aaagccattg gacaaggatt
     5941 gactgtattg ccaggtctga ctattgctaa tccagttctt ccttatatat ttggtaatct
     6001 catagcattc attttaccaa tcatttttat tctcgcttat gataaatata aagtgtttat
     6061 ccctgaagct aaacaccgta aatttgcaga aaaaactgac tcaaaaaaat tgccagttca
     6121 agtaaagtga gatgagagca tgttaaattt ttttagaaaa aatgaaataa gcgttgtagc
     6181 acctgcaacc ggtgtttttg aaaaactgga gaaattaagt gatccagtct tttcaaaagg
     6241 aatgatggga caaggatttg caattgatgc aaaaaacgaa acgattgtat ctccaatcga
     6301 tggttttgtt tcaagtattt ttcctacaaa acatgcctta ggaataaaaa caaaatcagg
     6361 aattgagata cttgttcatg taggaataga tacagttgag ctaaatggtg agggatttga
     6421 tattaaagtt caagaaggac aaaaagttaa ggctggggat attcttatga ccattaattt
     6481 tgtagttttt gaaaagaatc acaagactaa agatgtcatt gttgtttttc cagactacca
     6541 agacgaaatt ttcattccga aacttgaaga agtaaaacaa ggacaagaat tgtttgttct
     6601 tggctaatga taagtaatta ataatagaat cgaggaaaat gtatggtttt tggggctatt
     6661 gaagctggtg gaacaaaatt tgtttgtgcc attggagata aagatttgaa catatttgaa
     6721 aaagtcagct ttcccacgac tacacccaaa gaaacaatga atttagtttt agattttttt
     6781 aatcatcata aagatgattt agaaggtatc ggagtgggat cgttcggtcc tattgatatt
     6841 cattgtgaat ctaaaacgta tggcttcatt acatcaacgc caaaacttgc ttggcaggat
     6901 tttgatttta ttggaagttt aaaaaagcaa cttaatattc caactgcttg gacgactgat
     6961 gtaaatgcct cttgctatgg tgaatatgtt tctggaaatg gcagaggcct ttcaagtgtt
     7021 gtttactata ccgtaggaac gggcatagga ggtggcgcta tccaaaacgg aaacttcatt
     7081 gaaggtttta gtcatccaga aatgggacat atgcttgtaa aacgacacaa taaagacaac
     7141 ttttctggga catgtccatt ccacggagac tgtttagagg gcttagctgc tggtccggca
     7201 atcgaaagaa gatcagggaa aaagggacaa gaagtgactg aaaatgacgt tacttgggaa
     7261 atagaagctt attacattgc tcagtgtgca tataatacaa cattgatgtt atcacctgat
     7321 gtcattatat ttggtggcgg agtaatgaaa caaaaacact taaaaaagaa agtacaagat
     7381 aagtttattg agttgatcaa cggatatgtc gctattcccg atgttgacaa ttatattatg
     7441 acaccaaaat tgttagataa ttctggtatt gtaggttgtt tagctttagc aagagacgtt
     7501 aaagaaaaat cttaatttgg aaaaaagatc gtctcgacta tttgtttggt tgatgcgatc
     7561 tttttttcaa ttagtaattg aagggacgcc taaaagtgaa aaaaaaaatg aaagatgaag
     7621 tattagagta tatcaaaaat aatttaaagt attacgattt ttctgctcaa gatattgcaa
     7681 tgaaattttg tattaaaaga aatgtcgctt cccactattt gaatcagtta ttttcagatg
     7741 gaaaattact aaagaatgat tctgttagac cagtaatgtt caagtataat caacaaaagc
     7801 caaaagactg cttttctaag tttattggag cagacatatc tttgaaatcc actatagata
     7861 aatgcaaagc tactgtaatg tatcccccta atggcctccc attaataata aaaggaaaca
     7921 gtggcgttgg aaaaagtttt ttagcaagtt tgatctatca gtatgctctt gatagaaaag
     7981 taattcataa tgacgcaaaa tttgttgttg tgaattgtgc agattatgca aataatccag
     8041 agctattgtc agcagtttta tttggatata aaaaaggagc ctttactggg gctgaaaaag
     8101 atactgcagg gatgctatct tctgctaatg gaggatacct ttttttagat gaggttcaca
     8161 atctaagtgc agaaaaccaa gagaaattgt ttcttcttat ggattctcaa aagtatcgta
     8221 aacttggtga aagtgtgcaa tgggagtatg caaatgttcg ccttatctta gccacaacag
     8281 aagataaaaa tacctctttg ttagcaactt ttagaagacg catcccagct gaaatcactc
     8341 tgccagatta caataataga agtacatctg aaaaaattca attattattt gaatttctta
     8401 aagatgaagc aattacgatt gattcaaaaa tattttgttc gattgaactt tttactgatc
     8461 ttttatcgaa aacatttgaa ggaaatgttg gagaattgag gaatgaaatc aagcttcttt
     8521 gtgcagaagg ttatttagga aataagagaa agtcttcaat ttatttaggg gacaaactag
     8581 acagcggttt ttggatagat ccagaaatta gtatagatt
//
LOCUS       DARBER010000108         8572 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DARBER010000108 DARBER010000000
VERSION     DARBER010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.112"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="SCK06_002238"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="SCK06_002238"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1152201.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SCK06_002239"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SCK06_002239"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG1152202.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="SCK06_002240"
     CDS             complement(3070..4323)
                     /locus_tag="SCK06_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1152203.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="SCK06_002241"
     CDS             complement(4329..5252)
                     /locus_tag="SCK06_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1152204.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="SCK06_002242"
     CDS             complement(5473..7965)
                     /locus_tag="SCK06_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1152205.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="SCK06_002243"
     CDS             complement(7972..8439)
                     /locus_tag="SCK06_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG1152206.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1814 c         1422 g         2982 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DARBER010000109         8356 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DARBER010000109 DARBER010000000
VERSION     DARBER010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.113"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(777..2408)
                     /locus_tag="SCK06_002244"
     CDS             complement(777..2408)
                     /locus_tag="SCK06_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG1152207.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="SCK06_002245"
     CDS             complement(2422..4095)
                     /locus_tag="SCK06_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG1152208.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="SCK06_002246"
     CDS             complement(4108..4425)
                     /locus_tag="SCK06_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1152209.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="SCK06_002247"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="SCK06_002247"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1152210.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="SCK06_002248"
     CDS             6087..6872
                     /locus_tag="SCK06_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1152211.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="SCK06_002249"
     CDS             6875..7567
                     /locus_tag="SCK06_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1152212.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="SCK06_002250"
     CDS             complement(7841..8212)
                     /locus_tag="SCK06_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1152213.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2561 a         1828 c         1435 g         2532 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tctgagtgtt gattct
//
LOCUS       DARBER010000110         8318 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DARBER010000110 DARBER010000000
VERSION     DARBER010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.114"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..1339
                     /locus_tag="SCK06_002251"
     CDS             476..1339
                     /locus_tag="SCK06_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1152214.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="SCK06_002252"
     CDS             complement(1468..3090)
                     /locus_tag="SCK06_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1152215.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="SCK06_002253"
     CDS             3339..3860
                     /locus_tag="SCK06_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1152216.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCK06_002254"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCK06_002254"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG1152217.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="SCK06_002255"
     CDS             5941..6759
                     /locus_tag="SCK06_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG1152218.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="SCK06_002256"
     CDS             complement(6862..7059)
                     /locus_tag="SCK06_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152219.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="SCK06_002257"
     CDS             7228..7806
                     /locus_tag="SCK06_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1152220.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="SCK06_002258"
     CDS             7901..8038
                     /locus_tag="SCK06_002258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152221.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2503 a         1489 c         1642 g         2684 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttgg
//
LOCUS       DARBER010000111         8157 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARBER010000111 DARBER010000000
VERSION     DARBER010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.115"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            384..572
                     /locus_tag="SCK06_002259"
     CDS             384..572
                     /locus_tag="SCK06_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG1152222.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="SCK06_002260"
     CDS             650..1663
                     /locus_tag="SCK06_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1152223.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="SCK06_002261"
     CDS             complement(1730..2764)
                     /locus_tag="SCK06_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1152224.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2879..5104
                     /locus_tag="SCK06_002262"
     CDS             2879..5104
                     /locus_tag="SCK06_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1152225.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5122..7005
                     /locus_tag="SCK06_002263"
     CDS             5122..7005
                     /locus_tag="SCK06_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEG1152226.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6986..7960
                     /locus_tag="SCK06_002264"
     CDS             6986..7960
                     /locus_tag="SCK06_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG1152227.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2646 a         1479 c         1715 g         2317 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2821 cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat
     2881 ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt
     2941 cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt
     3001 ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg
     3061 aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga
     3121 tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat
     3181 cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc attatattac
     3241 agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc
     3301 acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta
     3361 taccatattt actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg
     3421 agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg
     3481 atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca
     3541 atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat
     3601 gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg
     3661 acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca
     3721 attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca
     3781 gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag
     3841 tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga
     3901 acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat
     3961 caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg
     4021 ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg
     4081 caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt
     4141 tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc
     4201 tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact
     4261 ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga
     4321 tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc
     4381 gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta
     4441 tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg
     4501 tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga
     4561 taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat
     4621 ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc
     4681 gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac
     4741 aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct atcaaacgat
     4801 tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat
     4861 tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat
     4921 actagcttca gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc
     4981 actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg
     5041 actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt
     5101 atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt
     5161 tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa
     5221 gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg
     5281 ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt
     5341 gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc
     5401 aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct
     5461 gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg
     5521 acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt
     5581 gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg
     5641 ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg
     5701 gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt
     5761 gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg
     5821 atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt
     5881 gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa
     5941 atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa
     6001 aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata
     6061 gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa
     6121 atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga
     6181 catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg
     6241 gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc
     6301 acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa
     6361 tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca
     6421 gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa
     6481 aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt
     6541 cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg
     6601 aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg
     6661 ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt
     6721 gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt
     6781 gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa
     6841 gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa
     6901 ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc
     6961 gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac
     7021 ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca
     7081 ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg
     7141 gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag
     7201 cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga
     7261 aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca
     7321 atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac
     7381 agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt
     7441 caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt
     7501 cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc
     7561 ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc
     7621 ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag
     7681 aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca
     7741 gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc
     7801 aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt
     7861 aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag
     7921 cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag attattattt
     7981 gaaaaacgga gtttacaaga caaagctgct ttattctata tgtcatgaac aagagagtaa
     8041 aaaagttctc agatactttt gttcatgact attttcttca ataaaaccaa ttgttatttt
     8101 tctgaaaaac aaagttaact atcagaaaat taaaaaaagt ttcataaaaa tgattga
//
LOCUS       DARBER010000112         8095 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARBER010000112 DARBER010000000
VERSION     DARBER010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.116"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            179..880
                     /locus_tag="SCK06_002265"
     CDS             179..880
                     /locus_tag="SCK06_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1152228.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1489..2505)
                     /gene="tsaD"
                     /locus_tag="SCK06_002266"
     CDS             complement(1489..2505)
                     /gene="tsaD"
                     /locus_tag="SCK06_002266"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG1152229.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2502..2966)
                     /gene="rimI"
                     /locus_tag="SCK06_002267"
     CDS             complement(2502..2966)
                     /gene="rimI"
                     /locus_tag="SCK06_002267"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1152230.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2973..3515)
                     /gene="rimI"
                     /locus_tag="SCK06_002268"
     CDS             complement(2973..3515)
                     /gene="rimI"
                     /locus_tag="SCK06_002268"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1152231.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3499..4221)
                     /gene="tsaB"
                     /locus_tag="SCK06_002269"
     CDS             complement(3499..4221)
                     /gene="tsaB"
                     /locus_tag="SCK06_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG1152232.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4412..5392)
                     /locus_tag="SCK06_002270"
     CDS             complement(4412..5392)
                     /locus_tag="SCK06_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1152233.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5416..6900)
                     /gene="galT"
                     /locus_tag="SCK06_002271"
     CDS             complement(5416..6900)
                     /gene="galT"
                     /locus_tag="SCK06_002271"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG1152234.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6912..7901)
                     /gene="galE"
                     /locus_tag="SCK06_002272"
     CDS             complement(6912..7901)
                     /gene="galE"
                     /locus_tag="SCK06_002272"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG1152235.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2298 a         1724 c         1417 g         2656 t
ORIGIN      
        1 tacacaaatc attcgcttca aaacaaatta ttcaatacca ttgtatattt tttacataat
       61 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
      121 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
      181 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
      241 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
      301 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
      361 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
      421 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
      481 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
      541 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
      601 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
      661 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
      721 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
      781 acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac
      841 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
      901 gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg
      961 gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat
     1021 attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg
     1081 atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga
     1141 acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg
     1201 taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac
     1261 ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa
     1321 ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc
     1381 aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc
     1441 ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt attcagataa
     1501 aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac
     1561 atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac
     1621 tagttctacc tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg
     1681 attggccgct acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg
     1741 aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt
     1801 tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt
     1861 taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta
     1921 attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc
     1981 taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat
     2041 gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag
     2101 aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc
     2161 catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga
     2221 aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa
     2281 atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc
     2341 tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat
     2401 ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt
     2461 ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt
     2521 tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt
     2581 ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga
     2641 cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac
     2701 aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg
     2761 acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa
     2821 agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt
     2881 gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca
     2941 tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata
     3001 tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac
     3061 cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc
     3121 gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt
     3181 cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac
     3241 gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga
     3301 ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta
     3361 tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt
     3421 acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa
     3481 aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt
     3541 ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt
     3601 ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc
     3661 ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa
     3721 tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa
     3781 caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca
     3841 gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt
     3901 atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt
     3961 atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga
     4021 accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc
     4081 aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg
     4141 tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt
     4201 atctattcct aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat
     4261 tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata
     4321 catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta
     4381 tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact
     4441 ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg
     4501 tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg
     4561 ttacatattt cgctacactg acatcattga agcccatcac agaaatatct tctggtacca
     4621 ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa
     4681 aaagcgcatc aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg
     4741 actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa
     4801 cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt
     4861 ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt
     4921 tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag
     4981 aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga
     5041 attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt
     5101 tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat
     5161 aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct
     5221 tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa
     5281 ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa
     5341 cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct
     5401 cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc
     5461 atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt
     5521 ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc
     5581 tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac
     5641 ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa
     5701 cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg
     5761 atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct
     5821 tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat
     5881 agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat
     5941 aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc
     6001 tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat
     6061 aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat
     6121 tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag
     6181 tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga
     6241 atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt
     6301 cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc
     6361 gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc
     6421 gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata
     6481 ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt
     6541 tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg
     6601 tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc
     6661 aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac
     6721 aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag
     6781 agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat
     6841 ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat
     6901 atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt
     6961 ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt
     7021 tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga
     7081 atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg
     7141 ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa
     7201 atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt
     7261 gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa
     7321 atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt
     7381 gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac
     7441 cacttcatca ttttttccat catcaattta ctttcaccat acggattttt aggattagct
     7501 ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag
     7561 aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat
     7621 acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca
     7681 gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta
     7741 tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg
     7801 agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc
     7861 gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt
     7921 agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga
     7981 aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta
     8041 aacattttca agggtttttt gcaaaggttt acaaacaaac gtaaaactag ccttt
//
LOCUS       DARBER010000113         7875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARBER010000113 DARBER010000000
VERSION     DARBER010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.117"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(22..996)
                     /locus_tag="SCK06_002273"
     CDS             complement(22..996)
                     /locus_tag="SCK06_002273"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1152236.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="SCK06_002274"
     CDS             complement(1076..1951)
                     /locus_tag="SCK06_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG1152237.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="SCK06_002275"
     CDS             2081..3988
                     /locus_tag="SCK06_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG1152238.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="SCK06_002276"
     CDS             complement(4529..7807)
                     /locus_tag="SCK06_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG1152239.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2219 a         1664 c         1463 g         2529 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtca
//
LOCUS       DARBER010000114         7760 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARBER010000114 DARBER010000000
VERSION     DARBER010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.118"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..315
                     /locus_tag="SCK06_002277"
     CDS             <1..315
                     /locus_tag="SCK06_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152240.1"
                     /translation="LKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVI
                     NGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRN
                     KL"
     gene            complement(441..1118)
                     /gene="nth"
                     /locus_tag="SCK06_002278"
     CDS             complement(441..1118)
                     /gene="nth"
                     /locus_tag="SCK06_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG1152241.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1125..1823)
                     /locus_tag="SCK06_002279"
     CDS             complement(1125..1823)
                     /locus_tag="SCK06_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1152242.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2091..3275
                     /locus_tag="SCK06_002280"
     CDS             2091..3275
                     /locus_tag="SCK06_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG1152243.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3281..3724)
                     /locus_tag="SCK06_002281"
     CDS             complement(3281..3724)
                     /locus_tag="SCK06_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1152244.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3950..4237)
                     /locus_tag="SCK06_002282"
     CDS             complement(3950..4237)
                     /locus_tag="SCK06_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152245.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4408..5760)
                     /locus_tag="SCK06_002283"
     CDS             complement(4408..5760)
                     /locus_tag="SCK06_002283"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG1152246.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6033..6479)
                     /locus_tag="SCK06_002284"
     CDS             complement(6033..6479)
                     /locus_tag="SCK06_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1152247.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6715..7641)
                     /gene="cysK"
                     /locus_tag="SCK06_002285"
     CDS             complement(6715..7641)
                     /gene="cysK"
                     /locus_tag="SCK06_002285"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG1152248.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2100 a         1578 c         1268 g         2814 t
ORIGIN      
        1 cttaaaggaa aatttatgga aagaagctct catgcaatga ttctcacttt tttatggttg
       61 ttgttcacca gaatctatat ttttctgatg agcctgaaac cgcccaaatc cattagttta
      121 tgtttagtta taaatggtat ttttttatta tcacttatca tttttattac acctttagat
      181 agatacgtca cttatttata taatcctttg gaatactgga cttattttat aggtatattg
      241 gaaggcatta ttttctacat cgggtatttt cccaataaaa ataacaactt ttatttttat
      301 cgcaataaat tgtaataagt taccatccta gctactgagt atgtacattt actttttcat
      361 gcaaaaaaga taaacgaaac agcttttctc cataataaca agataattca tacatttatt
      421 tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt ttagcgcctt ttcttttaga
      481 cgcatccgat ttttcccatc ttgacatatt gatagcaaag gacatacctc gcacttggga
      541 tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg tatgatgtgt ctttacccat
      601 aattcttgtg gtactttccg catcagtgtt tcttctactt ccatgaccgt agcatctagt
      661 ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg tatctactgc gattgctggt
      721 atcccgaatg catcccccaa tacgacattt gctgtctttc tgcctacacc aggaagagac
      781 atcaattctt ctcttgacgt tggcacttgc ccatcgaaac gttcaatcag ctgctgtgca
      841 cacgctttga tgttttttgc tttatttcga tacaaaccaa tcgtttttat tttaagaatg
      901 atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg gaaaagaggc aaacaaatct
      961 ggcgttgctt tattcactga tacatctgtc gcctgggcac ttaagatcac tgcaatcaat
     1021 aactcaaatg gattattgtg cttgagttct ccatgtgctt cgggaaacat tccatacatc
     1081 gtttctagag cttccattgt tttttgtttg cttaacatat tccgctactc ctccaaccaa
     1141 tttttcagtg agactttcgg caattcttct tgttgagctg actgttctac ttctctttga
     1201 agaagctgct gttttcttcg tttttggtct tcttccactt gttgttttgt acgtagattt
     1261 tttctttccc acgatagcaa aatgcggtca acgtaattaa aactataagc ctgatttaat
     1321 acggcttccc gcagtgctag cttcaaaatt tccggctgat aatggtcttc ttctagccat
     1381 tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc caaattcttg ttcaaataat
     1441 tgatacactg actggatttc tttttcttgt gttttttctt cgtatttttt ttcttgtgtt
     1501 tttaaatact cacctaatag atcatagatg gggtaaagat tatatcgatc tgctttttta
     1561 ccgttgacaa tcgtcgtttc tatcttgata aatccatttg ttacgagacg atccagtatt
     1621 tgaaaaatct gatcttgttt gattcccata tccatagcaa tcatctgcaa atcagggaaa
     1681 gtatctcctt caagctgtgc catatgcaac tgtagaacaa ataaaaattc ttcagattgt
     1741 agaccgatcc tatgataatt tttaagtaaa agattagata tgactgtttg tccagcgctt
     1801 aagtaatttt ctaaattcaa catgatttct cctccgctca atcacagtat acgtaaaaaa
     1861 aacatgggaa gcaagaacag gtttcttttc tatttacttc actgatcggt cagatttaca
     1921 atgtcgtcta cttgattttt gtttgttcat gtttttgatg cgtatcttca cttttcggaa
     1981 taaataaatt aagtagattt cgctgttcct tagaatgttg acaaggaaac aaaattacta
     2041 tacactattt ttgtttccaa gttaacatta ttgcataaag gagtttttac atgtacagtt
     2101 tcacaaataa acattctaaa gtttttggat acgtctctgt cttattcact ggattaggat
     2161 ttactatcgt cagtcctgta cttccgtttt taacgctgcc ttattctcac tccatacatc
     2221 agcaagcttt ctatatcact ttgctcatgt ctgtttatgc tctagctgcc ttcttatctg
     2281 cccccattct cggtagctta agtgatcatt ttgggcgtca tccgatcctt attatcagtt
     2341 tattaggatc aagtatcgga tatttgcttt ttggtttagg aaattcgata tggatgttgt
     2401 tccttggtcg tatcattgaa ggattgacgg caggagaaat ctccacctta tatgcttact
     2461 ttgccgacat cacggaacct aatgaaagaa ccaaagtttt tggatggatg ggtgctttag
     2521 tcggtatagg aacaacgctg ggtcctatta tagggggtct attagctgag ttggggaatt
     2581 ctgttcctat atttattgga gcactcttta ctttcttaaa tgccgtttat ggctacacat
     2641 ttatgctaga aagccttcca ataaaaaaac gttcagtaga tttaagtttc tcccatgtaa
     2701 gaccttttca ccaattacaa caattattca agatttcatc cgttattcct ttattgacag
     2761 caggttttgc tgtttggttg gcagctggct ctcttcaatc tatcttttct caattttcga
     2821 ttgacgcctt tcagtggaag gctgggttgg ttggtttaag cttctctttg attggaatct
     2881 tggatactgt ctcgcaattg ttcattatgc ctcgattgct caaaaaattt tcagaacagc
     2941 agatcacaag aatagggatg ttcagtgaaa tcctcgccta tctattcatc acattatctg
     3001 gaattcttct actgccgatt ttgtttttat ttgggattat ctgttatggt ttcggtgatt
     3061 ctatttttac tcctgtattc aatggccaac tatccaattc tgtctcagat aaccagcaag
     3121 gtcttgtaat gggcggtacc caaagtatac aatctctatc gcgcgtcatc ggcccgctaa
     3181 tcgcaggaca gctttatgct gcagcaccat ttcttccgac ggtattcgga tttttgctta
     3241 tgatttttgc ctattatcac tacaataaaa aatagtagct tcatttgcta cttcgtataa
     3301 attctctgta tcgaccgata aaacgaaata ctgtgtcaaa ttcttctttt gacatatttt
     3361 caaatacagt agcatctcgc tgtacccatt tttgatggag ttgctcgtgt ctattcctta
     3421 ttttttgtcc gattggcgtc aattgaaaat agatttcttt tttattctct tcgctttgat
     3481 aagtgtttat gatccctcta gctatcagtt tttttgttag cttactgatg gctcctctag
     3541 tcatgtaact agcattcgct aattttgtca cgttaggatc agggattttc ccgatatgtt
     3601 caatcacttc tagttcattt agagtaaatc ctgcaaattc tttttccatt tctggtttat
     3661 tacgatgaat aatctcatta gctaaatcca gatattcttc tattagcagt tgtcttttat
     3721 ccatttctac accttctttt tctttcatta tattgttttt ttgtttccta ttcaacaaag
     3781 ataaaaatat tttgtaggaa aacccaatct gaaaccaaga aaaaaagggg ttgacaaagc
     3841 ttgtcataac ccctttttct gattaaaaga tagtaaagag acagtcatcg gtaattgtga
     3901 tgattttttg tcatcactta aggatgagcg gccttttcaa aaactgtctt taaagatcat
     3961 catcagtttc tagcacatat ggctgacctt catatgtgta ctctgtattc aatgtgccgt
     4021 cattttcatc tgagtagatg cgtctactga aaggaaaacg atgagaaaca ttatctttgg
     4081 cgtcatttgc tggcatcgtg taatattcct cttgcgtttc attcaactga gagagtactt
     4141 gatctgccaa ctcatcaata ttgatcgtta atttcttcga agcactttta taatcttcta
     4201 aaaatgcatc ttgttggaca aaatattctt tattcatctt ctttctcacc tttcttttca
     4261 aaatcaaata aagctgcttg ttcttttatg taacagcttt atctttgctg cttattgtga
     4321 caaaaaattc gaagaaagac aactaattgt acttttcaca aaaaaagaca gagggtcgtg
     4381 acaaaattgt gtcacaaccc tttttcttta ttgtgcagtt tcgatttcgc ctagcgctgc
     4441 ttgtgcaagt aggttcaagt aattccatgg gcggtcaaaa tgtggttgga agaagaaatc
     4501 tgaaagtgcg agatcttcta cagtcatttt attttgaaca gctaaagaca tggtatttgc
     4561 tgattgtgta atgtcgtatt tggacatgaa ctgagcacct acgatacgat tcgttccttt
     4621 ttcatagact agctccatca ttactttttc agtagttggc atgaactctg gacgataatt
     4681 atcttcaaat acagtcgctt ggacttctag atcgttcaaa gttgcgcttt ctttggtcac
     4741 acctgtcgaa ccgatcgtcc agccaaatag gtaaagtcct gaagttcctt gagtaccacg
     4801 atacgccatt ttatgatctg tcaagttatt tcccacaagc aaaccttgac gaacggcatt
     4861 tgttgccaaa ggaatatagt tttgcgtgcc acttggattg tagttgacta ctgcagagtc
     4921 tccagctgca aaaatgtctg gattacttgt ctgcatgtat tcattcactt tgattgcccc
     4981 gtttggcagt gtatctactt ttccttcgat cagttttgta tttggacgga agcccacaca
     5041 taaaatgacc atatctgtat caaatgtatc tgttggtgta gctacttgtt tcacttttcc
     5101 ttcttcatct gtgataaatt cagtaacatt ttcgcctagc gcaagagtaa caccgcggtc
     5161 tgtcaattct ttttctaaga tatctgtgaa tggtttatct agatatttat tcaagatgcg
     5221 atctaatcca tcaatcaatg tcacttgttt tccagattcg acaaaagctt ctactaattc
     5281 aataccgata tatccaccac caacaattac tacacgtttt gcttctttcg cttgtgcaat
     5341 aatttcattt gcttgattgt agtttttgca taggagtaca tttttcgcat cgattccact
     5401 aatcggtggg ataataggcc atgaaccagt tgtcatcact aatttatcat aagaaaccgt
     5461 ttctgtttct cctgttttca gatcttttgc cgttactgtt ttattttctg tatcgacgtc
     5521 tgttacatcg tgttccattt taactgttgc tcctaatgaa gataattctt caggatttga
     5581 gtagaacaag cctgctgggt cctttactac tccgccgaca taaagtgcga taccgcaaga
     5641 taagaaagaa atattgtcat ttctttcata tactgtcact tctgcttctg gatgattttt
     5701 taaaatactt tttacagctg cagtacctgc atgagtacag ccaattacta ctactttcat
     5761 ttatggtccc tccgattaac attttgtcct tcgacaaaac tactataaca gaaataaaac
     5821 ggatacaaca gtttaatgct tgtgaattca caaaggtttg tttgtagttt cattcctttt
     5881 caagcataag aacccaatat ctacaagatt cttattttaa taatattcac ttttatatct
     5941 aagattgtga ataaaagaca aaaaaataaa aaagctagcg ttttttttac acagctagct
     6001 ttttttaggt ataacacttg tagaaactgt ttttacattt gcgccttttt tgctgactct
     6061 ttacaatccg gacagagtcc atacaattcc aaccgatgtt cattaatctc aaaatcagtc
     6121 agtctgcttg cagccatttc tatatcttca agccctgggt aatgaaaatc aacaatctta
     6181 ccgcagtttt cacaaattac atgataatga cgctttgaac tgaaatcaaa tcggctagaa
     6241 gcatcgccgt acttcatttc ttgaacaaat ccaatcgaag taaataagcg aaggttattg
     6301 taaacagtcg caacactcat atttggaaac cgatgttcaa gtgctttata aatctcatca
     6361 gccgttggat gggaatgatg ttcaatcaaa tattctaaaa ccgcgtaccg ttgaggtgtg
     6421 attcgaatgt tctctttttt taacttttcg ataacctctt cgactaactt attttccatg
     6481 taaacacccc acgttgttat tctcgtctta tcatactatt tttacatttt aaaatccact
     6541 taacatgaaa agaaaattca attttattgt attttaatga aaaaagacgt aaaacagtcg
     6601 cgttcactat tctacgtctc tattcaaata atttttctca tcataaattt ttgtgtgttc
     6661 ttttcatctt taaaagtttt ttacataaac ctatctggag aaaaaccaag tgccttatat
     6721 ctcttggtaa agggctgtag ataaataacg ttcaccattg tcgggaacca cagctaatac
     6781 cttcttgcct tttcctaatt cttttgcgac ttttagcgcg gcagcaattg cagcgccaga
     6841 tgagatgcct actaaaatcc cttcttttcg cccaacttct ctagctgttt ccatcgcttc
     6901 gtctccacta atagaaagga ctttatcata cacagaagta tccagtgttt taggaacaaa
     6961 acctgtaccg attccttgga ttttgtgagg tcccgcttct ttcccttcta aaatagcaga
     7021 ttctgctggt tctaccccga ttaattcgat tttcggatac acacgtttta attctcggcc
     7081 agcgccagtg atcgttcctc ctgtaccgat acctgcaaca aacgcgtcca agccatttac
     7141 tccaaatgct tgatgtatct caggaccagt cgtcttctca tgcacaagag gatttgcttc
     7201 attttcaaat tgtaatggta aaaagtagcc gttttctttt gccaaacgtt ctgcttctct
     7261 gattgatcca gaaataccat ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt
     7321 catcaataaa cgtctttcga tactcatcgt ctcaggcatc acgatgatta ctttatatcc
     7381 ttttgcaact cctaccatcg acaatccgat tcctgtattt ccagaagtag gttcaatgat
     7441 agtatcacct ggtttcagta gaccatcgtg ttctgctttt tctatcatac ttaaagcaat
     7501 acgatctttt acacttcctc ctggattaaa aaactcaagc tttacaaaaa catctgctga
     7561 atcttctggt acgattttat ttaatttgac gattggcgtt ttgccaatca attcggtgac
     7621 tgagttatag atagctgtca tcttcaattc ctccttgtct ttgctcttct tacttatcat
     7681 aatacaatct gttcatttat ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag
     7741 atataccata tatggtaatt
//
LOCUS       DARBER010000115         7621 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARBER010000115 DARBER010000000
VERSION     DARBER010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.119"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(75..1223)
                     /gene="nagA"
                     /locus_tag="SCK06_002286"
     CDS             complement(75..1223)
                     /gene="nagA"
                     /locus_tag="SCK06_002286"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG1152249.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1299..2135)
                     /locus_tag="SCK06_002287"
     CDS             complement(1299..2135)
                     /locus_tag="SCK06_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1152250.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2319..3536)
                     /locus_tag="SCK06_002288"
     CDS             complement(2319..3536)
                     /locus_tag="SCK06_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1152251.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3685..3864)
                     /locus_tag="SCK06_002289"
     CDS             complement(3685..3864)
                     /locus_tag="SCK06_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152252.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3919..4002
                     /locus_tag="SCK06_002290"
     tRNA            3919..4002
                     /locus_tag="SCK06_002290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3953..3955,aa:Leu,seq:gag)
     gene            complement(4242..5612)
                     /locus_tag="SCK06_002291"
     CDS             complement(4242..5612)
                     /locus_tag="SCK06_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152253.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6326..6616)
                     /locus_tag="SCK06_002292"
     CDS             complement(6326..6616)
                     /locus_tag="SCK06_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152254.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6824..7516)
                     /locus_tag="SCK06_002293"
     CDS             complement(6824..7516)
                     /locus_tag="SCK06_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1152255.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         2316 a         1516 c         1176 g         2613 t
ORIGIN      
        1 gatttttact agaacacaaa aaaaccttaa aaccaaactt aagaagttta gttttaaggt
       61 taacttaaat tatattatgc gtggaaacgt tccacgccat ctagataagt tgcttccaat
      121 gtcatatcag ggttcaatac gataaagtct gcatcgcgtc cttgagcgat ttttccacat
      181 gtatcatcga ttttacagct gattgctgga atcagactag ccatcatgac tgcttgttct
      241 ggtgttgcta atccccagtc tacaacattt ttaattgctt ctttcaattt caatatactt
      301 ccagctaagt tgccagattc tagacgtgct gttccttctt ttactactac tggaaattcg
      361 cctaaattgt aatttccatc aggcatccca ccggccatca tacagtctgt aattaatgcg
      421 acatgatcat gtcctgcttt ttccattagg attcccgcag catttggatg tacatgatgg
      481 ccatcacaaa tcaattcaga aaagacatgt tctaaagata ataacgcacc caccatacct
      541 ggttcgcgat gattcaatcc acgcatacca ttgaaagcat ggacaaatac acttgcgcct
      601 gcttcaacag ctttttgcgc ttcttctagt gtagcattac tgtgtcctag agaaacaact
      661 actccttctt cagtcacttg tttgacaaac tcttctacac cttgacgttc cggtgctaaa
      721 gcaatttttt tgattaaacc acctgaagct tcttgccatt catggaaagt atccaaatca
      781 ggatccccga aataacttgg gttttgtgcc cctttgtgtt cttctgtgaa gaaaggacct
      841 tcaaaataga ttcctttgat tttagctcct ggtgcttcct gatacatttt gccgattgtc
      901 tcagctacat cttttaaacg ttctttgctt gacgtcaatg ttgttggtaa aaatgaagtc
      961 actccacaag ataacagacc ttcagacatc actttgatgc cttctgcatc attatccatt
     1021 acatcatgat tcatatagcc gtgaatatgt gtatctacca aaccaggagc aatccatttg
     1081 ccgctttggt caatgatgtt gacttctccc tcaggtagtt ctttggtgta ttcaccaaaa
     1141 agtccatcgg taatttcaag atagcctgca tgtttgacac ctgacgctaa aaagaatttg
     1201 tcagcataga taaaagttct cattctattc tctccctttg tttttctacc tctaaagtac
     1261 tcttatttag agaagaagtc aagaatggtc tacaccattt acaaattttt tgaaatttgt
     1321 ttcacagctt gtgctaagcc ttcatagaat gctgctcttt ctggttcttc tttacgcttg
     1381 tatattaagc ttttaagcac aaataaatca ttgaggtaat agtagctatt cttctcttga
     1441 gcccaataga ttccttgatt cacctgttct aacgcttcgt cgatttcatt ttgatggaat
     1501 aagaaatttc cataattata aaaaatttgc gaaaattcac tttttgatcg ttcatcagct
     1561 gattgatgca gcagttggat gcttcgactc aaatagtagc gtgcttcagc cgtttttcct
     1621 gccacaccat atatttttcc gatacaacta atcagttgta tttctgtgtc agaaacatgg
     1681 attctttctt tttggctaga gtagcttaat gcttctttca aataataaag ggactgagag
     1741 atattttgac gcaacgtgaa ttcgcaaaca ccgagataat aataatagcg ttgaaagtca
     1801 gttgcttgat acagattttc agaaatatca gaagatttta gtacttgttc taacttccga
     1861 taatcacgtt tttggaaaca actatccaac agttcaaata atttatcgtt tgtccgagta
     1921 gtttttcgag agagattcat cacatgatca atcgttaccc ctagccgttg acaaatctgc
     1981 atcatcacca atacattcgg cagttcctca ttattttcga tccgacttaa tacactttgt
     2041 gaacaaatat catgcgaaag cattttttgc gtcatttttt gttcttttcg aatctcttta
     2101 atgatcgttc caaaggactc gattaacgtt tccataagta tcctccccaa aatatgcgta
     2161 ttcgcattct attcgtattt tactttagga agtcgcaaac ttcaagaaaa gacttgcaac
     2221 taaataatat actttagtta tttaagaaaa ctccactaga aagagagcct cagtgtgttt
     2281 gtctgtggcc ctcctactga aaaatttggt ttttaaaatc atttttggcg ataggttgct
     2341 aggaattctc tcattttctt agctgtttgt ttcaaatcat ccattttagg aagataaacg
     2401 attcgaaaat gatctggctg gttccaatta aatccaccac catgtacaag tagaatatga
     2461 tgttcatgta aaaagtccaa aacgaatttt tcatcatcca aaatattgaa tcgctttgta
     2521 tcaatttttg ggaatatata aaaagcagct tttggtttta cggctgaaag accaggaata
     2581 tcattgattg cattatagat atattctctt tgttcataga tccttccgcc tggcaggagc
     2641 agctcatcga cactttgata accgcctagc gcggtttgga taatttgctg agacaataca
     2701 ttggaacaca aacgcatgga ggacaacata ttcagccctt ctatatagcc tttgacgtgt
     2761 gacttatctc cacttaggac catccagcct actcgaaatc ctgctacacg atgagatttg
     2821 gatagaccgt tcagcgtaac aacgaacaaa tcaggcgcta atgtagcaat cggaatatgt
     2881 tctagaccat ccatcactaa gcgatcatag atttcgtcgg aataaataat caggttattt
     2941 tgtcgagcta cttcaacgat ttcttctaat aattcttttg gataaagtgc cccagtagga
     3001 ttattaggat taatgataac aatagctttt gtccgactcg ttatttttga tttgatatca
     3061 tcaatatcag gataccattc tgcctcttca tcgcaaatat aatgaacagg tgtgcctcca
     3121 gctaatgcaa tagaagccgt ccacaacgga tagtccggca ttggaaccag tacttcatct
     3181 ccattgtcca gcaacccctg catacacatc gtgatcaatt cgctcactcc gttgccggtg
     3241 tagatatcat tgatcgtcac attcggaaaa tgtttcagtt ggcaatattg ctcaattgct
     3301 tttctagcag aaaagatccc cttggaatct gagtagcctt ctgagtcacg ggcattcatg
     3361 atcaaatcac gaatgacttc gtttggtgct tcaaatccaa aaggtgcggg atttcccgtg
     3421 ttcaatttta aaatactaat gccctcttct tgcatccgct ccgcttcttc tagaacggga
     3481 ccgcgaacat cgtaactgac accttctaat ttgttggatt tttcaaattc tctcatcact
     3541 tagccaatcc ctcatttcaa aaagttagaa ctttccaaat tttttgttat ttactatttc
     3601 aatatcatac tccatcctag aaaattttgc cacttattta ggaaaaacgt aagtattaaa
     3661 aaaacacaaa caagcgttga gaacttaatt gtcatgatag tcattccata gattcgctgc
     3721 ccaatccatt gcaattccac aaatgattcc tagcataaaa ccaagcaaaa aacgtccgaa
     3781 tgcaagtcca atcaatacac ctaagatcat tcccagtcct gaaaattctc cgtcatattt
     3841 ctccatctta ataagtcctc ccatttttga gcttgacaac tatgtaaaac ctcgctatca
     3901 ttagtgtgtt attattacgc agatgtgttg gaattggcag acaagcatga ttgagggtca
     3961 tgtgagcgtt agctcgtgtg ggttcgagtc ccatcatctg cactacaact cgtgaagtta
     4021 attttgcttc acgagttttt ttgtttgaac gtttctttat gccattatat acatatcact
     4081 gtatccttta caaaaagaac gtacaatcat gtaaacagcc tgaggtgagt atatgaaggt
     4141 cggaaacaag ctactcgtag aaacttgcaa gtgttaaaac aattaatcga aaaacgagta
     4201 aaatccctca cctaatacta aagcgaggga ttttttgtat attatctgtt tatcaccgtt
     4261 attataaagg tacaaatacc aagcacggtt aatccaaatt gcataaattc atttttttgt
     4321 acactcatgc ttttcattag ctctttaact gccattagta ctaagatggc gataacaaca
     4381 taaagtatat agtacgtccg cattccggaa gcccatactg tgggagaaaa tcccataatt
     4441 aaccttgaac aaaatcccat tatcatcaaa ataactaaaa aaatagaagt tttcttattg
     4501 tcaaaactta aataaattcc aacaattaaa catagtaata atgccaagac tactaaagta
     4561 gctatccaag tacctggata aaaaagtgac agtttcgttc ctaactctgt aaagagatta
     4621 ttcaggttac tactactcca aatcattgcc cttttatttc cattgacgta agtaaagctt
     4681 tgacccattg tattaccaaa ataaataatc aggtttaaga aaaacggtaa tgcagtcaat
     4741 atacgcacat aatagttttg acatttccta tatgttagaa ggaaaattaa gaaaaatagc
     4801 aataaaaata agatattcat gtctaaaaaa aacggctttc caaatgatga aatacctaaa
     4861 tctaacttat taagaaagtt gaaattttta tattcaggaa accatttatt aatttcatgt
     4921 gcagcacgta atgcattccc tggagttgtc aaagagaaaa ttaattctgc taagcttatt
     4981 attgaaaata ctgataactt ataattatat cgtccattga caatcaaata taaactaact
     5041 atacttgtta gaacaaaaaa acaggcgttg acttgttctt gattagttaa aaatatcaat
     5101 aaaggaatac ttacccaata tatttttcta tttatttctt ctcctcttaa gagttgaaaa
     5161 aacggataaa aagttagtaa actaaatgct actggccact gatagttcaa ggtggttgct
     5221 ctccaaccgg tttcccccat tactgtcaat ggtattaaac agtatataga gcaaaccaat
     5281 aataaatttt taacatttat tttctcaaaa acatacttgc aaagtaatac tacaattcct
     5341 aacataatcg tagaatttaa taatttccat agcataaagt gtttagaaaa gaacattaag
     5401 aatagttcta taataattct tgaactccat gtcttataat gttctatcga agcacccaaa
     5461 tagccaagtt catctaatct acttgcataa actacgtcat ccgctctcat agtcggcata
     5521 tacatattaa agagtaaaaa gataccccaa atccctatta gtaacaagat cagttctttg
     5581 ttattcgtta tttttctatt atgtttgttc atttttatac tccttcttat atttcctaat
     5641 agagtataac atagctttaa gcattactta aagataactt aaaagttatc ttttaaatac
     5701 ttaattgtta aataccttat ttgatgcaaa agaaatctac gatagtcaac aaaaaaattt
     5761 tcattatatc cagaaaaatt tttcttagtt aaggacctat ggattgcagt aatgcagaat
     5821 tcgtcgaata aactgccaaa gacatacaat catattgctt ttaaaattga cgaacaagat
     5881 atagattcgt ttgtctcaaa aatccaaatg ttaggtttaa ctgtcgaacc aggacgttct
     5941 cgtgtgaaag gcgaggcttc ttcaatttac ttttacgact atgataatca cctcttcgag
     6001 ttacataccg gtactctcca ggaacgttta aaatcctaca attccactac ctgagactag
     6061 taatgactag tctcttttta tggcctctga tccccccatt gagcgaatac taatagggct
     6121 tcgaattgat ctgttcaatc tttaagcaat atccctcaaa tgagtttgga aagtttagtg
     6181 attcatcaca aatacttaaa tacatgtagt tatatataga ttgaggttct tgtgagcgta
     6241 tgctcgtgtg ggttcaaatc ccatcgtctg catccatctt tccaaaacaa gcggctagga
     6301 catttttgtc tcagccgctt gttttctagt ttattctttc atcttgtcgg accttttttc
     6361 ttgggtatag acttttctca tattctacca caattttggg tttccctaac acatgatagg
     6421 atctcacaac aaattgcttt ctattattat aataacctgt aacgactaac ttcatccctt
     6481 cgtctacttc agctaggaag ttcaaactat gggaagcaat caaacaattg accccattta
     6541 aactgaaacg gatcaacggt ctggcactaa cttgcaaagt acggatctta tctactgttc
     6601 catatttttt ctccataacc ttcacctcaa taccattata cgaacataca ttccctttgt
     6661 aaagaagaaa ctctgtttta tctttttgac tttttgtcta ttacctttct taaaatcata
     6721 ttggcacttc ctgcgaagat gaaggacatg tactaaaaaa aagaggcagc agtatagatt
     6781 actgttgctt ctttactaat ttaggtttac ttgttaagaa agattattta cgcaaatgtt
     6841 cttcaacaat ttgctgagat ttatcagctg tatcttccac ttcatctttt gctttttcta
     6901 acttttgaat atattgtttc aagcttgagt tttcagcagt tgcttcgtct aatttctttt
     6961 gtgtatctgt taactgagtt tctacatttg ttttttcttg tgtcagtgtt gcgatttgtt
     7021 gattcaaacc atttacagta ttttgtaatt ctgaaagttt tgcttgtgct tcttgtagtc
     7081 ttgctgtcca atctgcattc agatgattga tttcatcatt tttttggttg atctcatcta
     7141 gtttgacatt gatttcattt tgtttgcttt gtagttgtgc atccttgctg actaattgac
     7201 tttctaagtt ttgtttttct gctgccgatt gatttttttg gttttctaat tcatttactt
     7261 ttgtttggta ttcttggaca tctgcctttg aatcttgcaa tgtctgttct ttttgtgcta
     7321 gtcgagcggt aagatctgta agcgcttgct ctttatctgt caatgtatga tcaagctgtg
     7381 tcagattctc attgattgta tcaatatctg tctctccacc ccacaaaata atttcatttg
     7441 cgtaccgcgt gcctgtgtag ccaattagac atagaaatgc aacgacaatc ccaccagtaa
     7501 taaatttctt tttcatttgc atagctcctt aatccaatac ttttacatgc taaaagtaag
     7561 tgatctcaaa cacaatgtca agcgttttta aattacatgt gtaattttaa tataaatact
     7621 a
//
LOCUS       DARBER010000116         7618 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARBER010000116 DARBER010000000
VERSION     DARBER010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.120"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..790)
                     /locus_tag="SCK06_002294"
     CDS             complement(62..790)
                     /locus_tag="SCK06_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1152256.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(803..2083)
                     /locus_tag="SCK06_002295"
     CDS             complement(803..2083)
                     /locus_tag="SCK06_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1152257.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2224..2826)
                     /locus_tag="SCK06_002296"
     CDS             complement(2224..2826)
                     /locus_tag="SCK06_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1152258.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3071..3277
                     /locus_tag="SCK06_002297"
     CDS             3071..3277
                     /locus_tag="SCK06_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152259.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3589..4308
                     /locus_tag="SCK06_002298"
     CDS             3589..4308
                     /locus_tag="SCK06_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1152260.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4389..5072)
                     /locus_tag="SCK06_002299"
     CDS             complement(4389..5072)
                     /locus_tag="SCK06_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG1152261.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5290..7476
                     /locus_tag="SCK06_002300"
     CDS             5290..7476
                     /locus_tag="SCK06_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1152262.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2730 a         1283 c         1435 g         2170 t
ORIGIN      
        1 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
       61 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
      121 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
      181 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
      241 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
      301 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
      361 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
      421 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
      481 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
      541 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
      601 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
      661 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
      721 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
      781 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
      841 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
      901 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
      961 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     1021 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     1081 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     1141 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     1201 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     1261 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     1321 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     1381 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     1441 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     1501 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     1561 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     1621 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     1681 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     1741 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgt
     1801 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     1861 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     1921 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     1981 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     2041 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     2101 gatttttacc aaattatagc acaattagcg tattattttt ttaatttttc agggattttg
     2161 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     2221 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     2281 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     2341 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     2401 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     2461 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     2521 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     2581 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     2641 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     2701 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
     2761 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     2821 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     2881 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     2941 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     3001 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     3061 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     3121 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     3181 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     3241 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     3301 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     3361 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     3421 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     3481 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     3541 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     3601 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     3661 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     3721 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     3781 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     3841 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     3901 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     3961 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     4021 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     4081 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     4141 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     4201 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     4261 tacactacaa ataacccgaa agcaaaggga atctttacaa ataaataata taaaaactaa
     4321 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     4381 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     4441 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     4501 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     4561 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     4621 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     4681 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     4741 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     4801 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     4861 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     4921 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     4981 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     5041 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     5101 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     5161 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
     5221 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
     5281 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
     5341 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
     5401 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
     5461 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
     5521 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
     5581 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
     5641 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
     5701 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
     5761 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
     5821 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
     5881 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
     5941 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
     6001 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
     6061 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
     6121 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
     6181 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
     6241 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
     6301 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
     6361 aaaactcttt tcttattgac taattatcgt tctcgggcag ctattgtcca aggagcaaat
     6421 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
     6481 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
     6541 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
     6601 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
     6661 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
     6721 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
     6781 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
     6841 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
     6901 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
     6961 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
     7021 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
     7081 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
     7141 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
     7201 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
     7261 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagtg
     7321 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
     7381 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
     7441 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
     7501 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
     7561 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa agggaggt
//
LOCUS       DARBER010000117         7533 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARBER010000117 DARBER010000000
VERSION     DARBER010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.121"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(876..1289)
                     /locus_tag="SCK06_002301"
     CDS             complement(876..1289)
                     /locus_tag="SCK06_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG1152263.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1745..2230)
                     /locus_tag="SCK06_002302"
     CDS             complement(1745..2230)
                     /locus_tag="SCK06_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152264.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2369..2584)
                     /locus_tag="SCK06_002303"
     CDS             complement(2369..2584)
                     /locus_tag="SCK06_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG1152265.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2808..3608
                     /locus_tag="SCK06_002304"
     CDS             2808..3608
                     /locus_tag="SCK06_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1152266.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3627..5495
                     /locus_tag="SCK06_002305"
     CDS             3627..5495
                     /locus_tag="SCK06_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1152267.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5488..5979
                     /locus_tag="SCK06_002306"
     CDS             5488..5979
                     /locus_tag="SCK06_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG1152268.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5967..6521
                     /gene="srlA"
                     /locus_tag="SCK06_002307"
     CDS             5967..6521
                     /gene="srlA"
                     /locus_tag="SCK06_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG1152269.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6539..>7533
                     /locus_tag="SCK06_002308"
     CDS             6539..>7533
                     /locus_tag="SCK06_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG1152270.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2682 a         1208 c         1432 g         2211 t
ORIGIN      
        1 aaagataaca cccaactttc aaaaatatgt tttggaagtt gggacaaacg agttaataca
       61 ttttaataca ttatttttta ttcaaaaaaa atagcacaat ggcaaagatc gtaagagaga
      121 tcataagccc caatgaatga ctaaaaagaa aagcagaatc acatgccata tcatcccata
      181 tttcttcaag aaaataaata agcgggaaaa agggaaggaa tagtagaaca tcgatccagc
      241 accatttttt tcttcttttg aaataacata aaacaataaa catgaacgtg cagggaagta
      301 aaaacaaagc tttactcata actaacaaaa taccagctcc ttttattttt ttctccccca
      361 cattcaaaga gtaacaatgg tatgtactta ataatagaaa aatggatcaa cttgtatgaa
      421 taacagctga aaagatctgc accaaataaa aaacttccag ccaaaactga ctggaagttt
      481 atttttttct gaaatattct aacacaaata tgaaaaagat ggccgaagct aataagccga
      541 tttcgtagca aaacaatgag ataccagagt cgtaataata aaatcctcta atcttatgga
      601 taaaatagat catcggacaa gcagataaga gtaagtattt gttaggcctt ttgaataagt
      661 aaaagaaagc actggtcatt atgataaatg gtatccaaaa caaaagttta cttaagacaa
      721 tcaaatgatc aactcccata ttttttacca agagagatca taacatcttt atcgtaaaaa
      781 caaaatattt ttttgctcat tttgaaaatt taaacatact cgttcttatc caaatatata
      841 aaaatcgccc ctcattcatc gagaggcgat aagttctaat ttaactcaat tttttttgtt
      901 tcttttggct gataagctgt tttaggtaat accaatttca gcacaccgtt tttaaaggtt
      961 ccagtgattt tttcttcatc aacattagga agatagaatg aacgtttgta agagaccgaa
     1021 tgtctttctt tccgtacata gttgccatct tctttatctt ctgtatggga ctcatgattg
     1081 gcggagatcg tcaaagtatt atctgaatat tcaactgtcg tgtcttcttt gtcacagcca
     1141 ggcatatcag cagttagttt gtattctttt tcattttcga ctaaatctac ttttggataa
     1201 cttgaaacac ctaaaaaatc attgaatgct ggactaaaca cgtcattgaa atcagggaac
     1261 atgtcgcgaa tgctaggtac atttgccatt tttgatcatc ctttcaataa gtatttttta
     1321 tttatcccta tttacgtaat aactctattt tgaaaaaaat gcaagtataa tggttagctt
     1381 tgtgaatatt tcatctaata cgatacaaag gctatctttc taatttgaaa aagggatcat
     1441 taaaataata agaagaaaat atgtcaaaag gtaaaaaagt aaattcttct atccaaatat
     1501 agaaaaattg aatctagaaa accgcccgac aataaatttc attaactcag ttgaccgaaa
     1561 atgaagtaga taccaaaaat ttatcaagag agcaagtaga gaagtgagta tattcataag
     1621 agaaatagct gtaacgaatt attaggttaa tgcaaatgct tatttatact ccgtataact
     1681 ttctcagaaa aaatgatacc aataatgact tgctagtact gtaaatccgt ataatatcat
     1741 ctaatcaaaa tggtgttcca tttgcatctg tcatcgtaat cgatccttcg ggtgacactc
     1801 gataaaaacc aacgcttcca gatcctcctt gggaacggat ttgtttgctg gaagctgtga
     1861 acaaataatc tcgatcaatt ttttgataaa agctaataac aatatcgtca ctttgtaatt
     1921 cggaacggtg ggcaataaga aatagaagtg ctttgtcaga gttttctaat ggaagagaag
     1981 gtacagtgct ttcttgcatt tcagatgttg aagaaatgct aaacgactga atacttgatt
     2041 ggctatgttg cgaatctaca ttaccagtag aagtattagt aatggaagta gtagaagtac
     2101 ttttagttga accaacctca gttgttgaaa tggctgctga agattcttta acagaattct
     2161 ttgttttata tccacaggca gaaaaaagta ttacagttcc caacaagcct gcgtaaatcc
     2221 ctttatgcat gatagctcct cctaaaatag attatcttta ttcagtatat cacttaaaca
     2281 aagaaaagca gatggtttaa aatgaaaaaa ccttcccaat aaagggaagg ttcatgaaga
     2341 taataacttt tttaataatt atagctttct aatatctttc atcaactttt ctagtaatca
     2401 ttgggaaaaa tttcaagcag tagatcgcac aaatacctac gacgatataa atgatcttcg
     2461 caaaaatagt tgttgaacca ccagcaatca tagctactaa atcaagttcg aacaagccta
     2521 ccaacagcca gttcaaccct cccacaatca ataaagttaa tgcaatagca tctaaagttt
     2581 tcattagaat tctcctttct taaatgataa aacaattata tatcaaaaag agaaacattg
     2641 cttatataac gctcaggaat tttagcaatc tatgcaaaaa gatgaaaatt ttttttgctc
     2701 ttccattagt acacattttt tagctgaagc tgtaaatttc atgtcttaat tttttttagt
     2761 gagcttttat actggatttg taagaaaaaa gaaggagagg atcacatatg tcggattggt
     2821 tggatattgc aggaaaagta gtaattgtaa ctggaggttc ctcaggaatt ggaagaagta
     2881 tcgtcgaaaa tttgttgaag cagaatgcac aagtagcgaa ttttgatgta actgaatgcc
     2941 gtatacagca tgaaaaccta ctatctttaa aagtcgatgt gtcttctaaa acggacataa
     3001 aagaagggat atataaagta atgaaacatt ttaagacgat cgatggtcta gtcaataatg
     3061 caggaataaa tattccttct cttctaatag atagaaatca tccaaaaagc aagtatgaat
     3121 tatcagaaca agtttttgat aagatgatcg ctgtcaatca aaaaagtgta tatttgatga
     3181 gccaagcagt tggaagaatc ttggtgcaaa agggatcagg agtgatcgtg aatctttctt
     3241 ctgaatcagg attggaagga tcagaaggac agagctgtta tgctgctaca aaagcagcga
     3301 tgaacagttt tactcgttct tgggcaaaag agttaggaaa gaggaatgtt cgtgtggtag
     3361 gtgttgcacc agggatactg gaagaaaccg gtttgagaac acaagaatat gaagaagctc
     3421 tttcttatac gagaggtata agcgtcgaac agctgagaaa cggttacagt cgaacaagta
     3481 cgatccctct aggaaggagt ggtaagctgc aagaagtggc ggatctagtc tgctattatc
     3541 tttcagaaag atcaagttat atcactggag tcacgactaa tatttcagga ggtaaaacaa
     3601 gaggataaag gatagggggg acgcacgtga cgctagtaaa cagatggtac caattattga
     3661 aattactagt cactcacaag aaagtttcaa tccaggaagt gcaaaaagaa atcaaaacca
     3721 gtaatcaaac cataaaaaaa gatattgggg agttgaatga agaaattgcc ggtattgcaa
     3781 aaatcatcca aaaaaacaaa cattttgaac ttgaaattta tgattttgat ttttttgatg
     3841 agatcatgca aggaaaattg aaaacagcca gtgatttcaa ttcggcatcc aaaagaatcg
     3901 cttatttact aaataggctg atcgaaacag aggatttctt acgtatggat gatttgtctg
     3961 aagaagtcgg tgtcagcaga ggaacagtgg tcaaggatct aaagacatta aaagagatga
     4021 tcaatgattt tgatgtaaag attacaggca caccaaataa aggactaaga ataataggag
     4081 atgagctaga attacgacta cttttatttt attttgtttc tccctacttt tcagagacga
     4141 aaacagaaca gtttctagaa gaaaaacaag caatcaaaca ttttcagaag aagacgaatg
     4201 ctagtaaaca agaaatcacc cttttaacga aagttatcgg tatttctttg aatagaatct
     4261 ctagtaacta tctgttgaag aagcccatca atcgttacag tgggtgtttt gaatatatcc
     4321 aagaattcaa tgaactgatc aatcagttgg aagaaatcta tgaactaact ttgagccaat
     4381 atgaaaaaga ttttatcagt tttcctttga acatcatcaa cacaagtata acgatgataa
     4441 ggaaagctaa ctctgatttg cgcttatatt tcgaccccat gatgatgcgt attcgacgat
     4501 tagatctcat tgatctggat gagtccttct tatatcaaga gatgaaacag cacctctctt
     4561 tattgataaa tcgggtaatt tttcgttatc gattgactga tttattttat ggtgagatag
     4621 aaaaaaagta tcctttttct taccaaatcg ctagcgaatg catacaagca ttagaagcta
     4681 tcttgcaaca taagatctct aaagtagaga tcagctacct aacgttgtat ttggagttag
     4741 catttcgata tcaaaaagag catttttcct ctaaaaaagt agccattgtt catacgacag
     4801 gaaaaggaac agcattaatg atccaaagac aaatcaaaag gatattgggg agggatatac
     4861 aaataacaca tgtttcagaa gaacaatatg aaaaagtaga tctaaacaaa tatttcgctg
     4921 tttttacaac gattccgtta aaaaatgtaa atgaagagac accggtgatc catttgacta
     4981 atctttttga tgactcttgg ttaagggcac aatggcaaaa agcaaaaaaa aagcgattag
     5041 ccgggtttca ttctactgtt tttttctatc aaatatggaa agaaaaaaag acgtatcaag
     5101 aatatttaaa caaattagtc gctcctttga tcagaggcta ttatgtggat gaagaatttg
     5161 aaaaaaggat cttgaaaaga gaatatcagc aaagcacatt tgttaacaaa gaatttgctt
     5221 ttcctcacac aaccaaccag ctatctcaac acattgtttt atcagtaggg ctaataactg
     5281 atcaaggttt agcagaaaca ggaggaaatg gagttatttt tttatttgcc atacctgaag
     5341 aaatgacggc tgaagttgag aatgaattat ttgagctata cgatatccta tttgaactca
     5401 taaaaatggg aatagatgag tttattgaat tagcaccgac acaagaatca tttttaaaac
     5461 taatcaaaag taaaggagag agacttcatg aatgatatgt atctattcat agttttcgca
     5521 gcttcagctt atgtgctgca agcagttatc agttggaagc aactaaagca tttcaatcaa
     5581 acatatagcg aactgcgaag aaaaggaaaa gtagcgattg gtaaacgacc tgggaaaatc
     5641 cgttcaggca cgattgttat gctagctata gacgatcatg atcaagtttt agaagtcaga
     5701 aaaatgcaag gggttacatc tttggcaaaa tttcattcat tacctgtcta tgcgggtgaa
     5761 gatatccatt acatcgatcg ttaccatccc tcagttcgag cggaagatcg tttgacacaa
     5821 gcagctttag aaaatgccag agaagtatac ttacgaatag aggcaggaaa tgaaaaaagt
     5881 gaacaacctt cattgccttt tattgctatg ctcaataatt ttcggatatg gaaaagtacg
     5941 atcaaagaaa aaaggagtga caaacgatgg atatcataac aaaatttgct gagggattta
     6001 tgaggctgtt ccaaacaggt gcagaaacat tcataagctg gatgacagga atcgtaccag
     6061 ttgtcttgat gctgatggtt gcaatgaata cgctgattgc ttttttaggt gaagaccgag
     6121 taacgaaagt agccaaatta tcagcgaaaa atccgcttac tcgttacctt gtgttaccgt
     6181 tcttatctgc ttttatgttg ggaaatccta tgtcatttac aatggctcgt ttccttccag
     6241 aatactacaa accaagctat tatgctgctc aagctcaatt ttgtcatacg agcaatggtg
     6301 tattccccca tattaatcct ggagaattgt ttgtatggat ggggattgct caagggatcg
     6361 aaaccttagg gctaaattcg atggagctgg ctatccgata cttgcttgtg ggactgctga
     6421 tgaactttat tggtggttgg ataactgatt ttactactgg atttgtatgc agacagcaag
     6481 gaatcgttct tagcaaaaaa gtagagcttt cagttgatta gagaaaggag agttttaaat
     6541 gtatggtgat attaagatcg taagaggaaa cggcgggtat ggaggtcctt tgattattag
     6601 gccgacaaaa gataaacaca agttcatcta tgtcacaggt ggaggtgaga ggccaatgat
     6661 cgtagataaa atagaagaac tgacaggtat ggagtctatc aatgggttca aaacatcaat
     6721 acctgatagt gaaatcgcgc ttgcaatcgt tgactgtgga gggactctta gatgtggaat
     6781 ttatcctaaa aaagggatac caaccatcaa tatcgttccc acaggaaaaa gtggcccatt
     6841 agcacaatat atcactgaag atatttatgt ttcagcagta gggttagaac aaatacagcc
     6901 actaatggaa actgatgata acaaagaaac agtaaatact gcatctccag ataaaaagta
     6961 caaatatgat acttctaaaa agataaccga gcaaaaagca gagcagcaaa actggatagc
     7021 aaaaattgga atgggcgcgg gaaaagtagt tgctacattc aatcaatcag caagagaagc
     7081 agtagatacg atgatccgga cagtgatccc ttttatggct ttcgtttccc ttttgattgg
     7141 tgtgatacaa ggttctggga tcggtaatgt atttgctaac attatggcac ctttagcagg
     7201 aaatgcagtt ggattaacaa tcattggctt tatttgttca ctgccatttt tatctccttt
     7261 gcttggtcca ggggcagtta ttagccaagt gattggaaca ttgatcggtg tagagattgg
     7321 gaaaggaaat attcctcccc aattagcatt accggcacta tttgctatca atactcaaaa
     7381 tgcatgtgat tttattccag tcggtctagg tttggaagaa gcaaaagctg agacagtaga
     7441 agtaggtgta ccatcagttc tttattcaag atttcttaac ggcatcccac gagttatcat
     7501 tgcttgggta gcaagttttg gattatatag ctg
//
LOCUS       DARBER010000118         7511 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DARBER010000118 DARBER010000000
VERSION     DARBER010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.122"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..179)
                     /locus_tag="SCK06_002309"
     CDS             complement(<1..179)
                     /locus_tag="SCK06_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1152271.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="SCK06_002310"
     CDS             complement(192..1295)
                     /locus_tag="SCK06_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1152272.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="SCK06_002311"
     CDS             complement(1385..2341)
                     /locus_tag="SCK06_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEG1152273.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="SCK06_002312"
     CDS             2622..4556
                     /locus_tag="SCK06_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1152274.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="SCK06_002313"
     CDS             5013..5429
                     /locus_tag="SCK06_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1152275.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="SCK06_002314"
     CDS             5443..6009
                     /locus_tag="SCK06_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG1152276.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="SCK06_002315"
     CDS             6032..7018
                     /locus_tag="SCK06_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1152277.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7511)
                     /locus_tag="SCK06_002316"
     CDS             complement(7173..>7511)
                     /locus_tag="SCK06_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1152278.1"
                     /translation="SDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHA
                     SVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRI
                     TFMSYKKSKR"
BASE COUNT         2167 a         1439 c         1538 g         2367 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga cctttacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagcaatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac t
//
LOCUS       DARBER010000119         7395 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DARBER010000119 DARBER010000000
VERSION     DARBER010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.123"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..878)
                     /locus_tag="SCK06_002317"
                     /pseudo
     CDS             complement(1..878)
                     /locus_tag="SCK06_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(961..2070)
                     /locus_tag="SCK06_002318"
     CDS             complement(961..2070)
                     /locus_tag="SCK06_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1152279.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            complement(2364..3389)
                     /locus_tag="SCK06_002319"
     CDS             complement(2364..3389)
                     /locus_tag="SCK06_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1152280.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(3634..4905)
                     /locus_tag="SCK06_002320"
     CDS             complement(3634..4905)
                     /locus_tag="SCK06_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1152281.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(5189..6136)
                     /locus_tag="SCK06_002321"
                     /pseudo
     CDS             complement(5189..6136)
                     /locus_tag="SCK06_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            6148..6621
                     /locus_tag="SCK06_002322"
     CDS             6148..6621
                     /locus_tag="SCK06_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1152282.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(6794..7348)
                     /locus_tag="SCK06_002323"
     CDS             complement(6794..7348)
                     /locus_tag="SCK06_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1152283.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         2316 a         1442 c         1094 g         2543 t
ORIGIN      
        1 ttctgcaact tcatttgaat tgcagtaata attaatataa tcactaaaag gaacataacg
       61 attgccgaac catatccctt gtcatttaaa ataaatgaat gtttatagaa taaataggct
      121 aatgattgtg atttaaacat ggcggtacta ttaggagtaa tcatcataaa aataacatca
      181 aatacttgga aagcattgat aactgttgtt acaactacaa aaaataatgt tggtgtaact
      241 aatggtaacg taatgttgaa aaactgctgg attggactag caccatcaat ttccgctgct
      301 tcataataat ctttaggaat ttcttataag ccggctagta ataacaccat gttataacca
      361 atagcactcc aaactcctac aatgataata gaataaatcg caatcgatgg atccgttaac
      421 catgctggtc catcgagacc tattttagcc aacatgttat taatcagtcc ataatcatta
      481 ttgaacatcc agcgccaaat cattgcgact gcggatggtg cagcaacgac tggtagaaaa
      541 taaattgtcc gataaatcga caatccttta acttttctat tcattaaaac tgccaccaac
      601 aaggataata caacaatact aggtatcgaa accactgcat aaattaatgt gtttaataaa
      661 gactgaaaaa cttcaccgtc tgcaaacatt ttttggtaat ttcttaatcc gacccattca
      721 gtatcaccga accctaccgt tttttgaaaa cttaaaattg ctgtttgaat tgctggccat
      781 atatttaata aaattaatcc taatgccgtt ggaaaaataa aaatatagcc ccatatcact
      841 tgatctttac gatattttcc aaaaattcga tttttcataa tatccccctt tttggtattc
      901 atccattaat atacagacga aatagaatcc tttcgcctgt atattgtata attaactaat
      961 ttaatttccg ctcaatatcg cattgacttc tttttcaatt tgagcggttc catcttcaac
     1021 agttacttca ccagacagaa tacggttaat aatttgaact tctgcttgtt cagctcgtaa
     1081 tccatcttta ttgtatggac gagggactcc atactccaac atttttccaa aagcactcaa
     1141 atcaattgta tctgaagaag cagcccactc gtcttcaaag ccttttagtg ctgggattga
     1201 tgcccccgtt tccgcttgga ttttattggc gtcctcacta gctaaaaatg ccattagttt
     1261 tttcacttct tctgggtatt ttgtactcgc actggcagcc caaccaagtc catttgtaat
     1321 tgttgcatta acttttccct taggtaacac ggcagaagca acattttcaa ctagatagtc
     1381 atttttcaaa aatcccggtg caaaccagtt tccttcaatc atcattgcta atctacctga
     1441 ttgaaaacga cttaacttat caggatcttg taattctgta attgttggtg aagcatgatc
     1501 aacaagtgca atatctcgat aaaaagctaa agcttcttgt gcttctgggg tattcaaacc
     1561 tgattttgta ttgtcatcgg caaatacttc tcccccattt tgataaataa atggccagta
     1621 gccagtctct gttccaatat gtgatgcaaa accatagatg ccttttgatt catctgttaa
     1681 atcaattgca gcttgtctga gtgtttccca tgtccatgtt tcatctggat aatcgacacc
     1741 tgcattatca aataattctt tgttgtacca tagccccact acagaagcat ctttagggat
     1801 tgcgtatagt tcgtcttcaa gcgtatagaa atcagcaatt ccttcagtaa agtttcccat
     1861 gtcaatatca ttttctttga taacatcatc aagtcccatc aaagcattct gtgacatata
     1921 tttataggat tcgctagagt gcatggttac tacatctggc atattacctc ccgttgttgc
     1981 agcttctaat ttaacccaat attgatccca aggcgttgtc tccatttcta ctttgatatc
     2041 aggattttgt ttttcaaatt catctaacat ttttcttagt cctggagctt ggtcagtatc
     2101 tcaactcgca tagcttaaag ttacttgccc gtctttagaa ttactatctc ccccaccaca
     2161 tgctgcgagt aatgtcccag ttaaaacagc tatccctgtt aaagctattt ttttgaaatg
     2221 tttcatctta attcctcctg ttttgaatgc gttttctttt tgttaacaac accataactg
     2281 tttacttaat gttttgtcga gattttttac taaaaatatt agtaaacaaa aaagtaaaca
     2341 cccttttttg gatgtttact tttttaatcg atattttttt cactttctct aacaataata
     2401 ttattagaaa caatcacttt gatcgaaaca tttctaactt cttttattcg ttcattaacc
     2461 atgcgaactg ctagttttcc aatttcttct gtattcacat tcaccgtatt taatgcaggt
     2521 gttaaaaatt cagcaacttc aatattatca aaactcacaa tcgaaatatc ttctggaata
     2581 tgtagaccat ttttttgaag tgaacgataa acccccacag caataggatc atttcctacc
     2641 atgatagcag tcggcaactt atctttagca tgttttttca aaagtaaatc tgctgcctgc
     2701 attccgtcaa gtgtggtcca gccttctaaa taaatttgac tatatttttc taatcctttt
     2761 tgtttcatcc acgtctcata ggctattttt cgataatcat catctgacac gtgtttattt
     2821 ccttggctat ctaaacgaac acgtcctcct ccgataaacg caatgtttcg gtgtcctttt
     2881 ttatacaatc gatctaaatg ctgattcgtt gcttttgtta aatcagtata aactgcatct
     2941 acttcagctt caatactagg gtcatcaatc actacgacat tcgaattaat tgctgatact
     3001 tcttcaatca cagaagatag cacctgccca ataatcaaca caccttgaca ttttagcaaa
     3061 tcgtcttgct ccaacggtgt ttctgacaaa cgaataattt tcttcaaatt gactttgctt
     3121 tgttctgctt cgacttgaat ccctctgcga atcgttcgaa aatagggatc ttccaactca
     3181 tctacttcag aaactgtcgt tattaatgca atatttgtta aattttttgc aacgggactt
     3241 tttttgacat aatgataaaa attaaccgat tctgcaatct ttcgtttggt ttcatcggtt
     3301 actgaaagtg tcggatcctc atttagcacg cgcgatactg ttgcaatgga cacacccgcc
     3361 cgttttgcaa catccctaat tcctaccata atagctcctc ttttcatatt tgaaagtgtt
     3421 tactaacata taatcataca ttttgaaaat aagcaaattt cgttaaaaat aggcactgta
     3481 atcataatta aaaacaagaa aaaatatccg aacaatttag agtaaataat gctaattcta
     3541 gtgaatttgc gacgaggaac tgctagatca cagttcattc tctctatcgt tcggatattt
     3601 tattgttaac tatacttatt tcacagagct tttttatttg attttgtgaa cagagtctcg
     3661 agaaatcaat tctccctcta atagtagtac ttgatttttt atttcttctt gtttggccag
     3721 tccaatcagt tggtgaaaag caatcgcccc cttgttacct ggatttagtc gtattgttgt
     3781 caacgcagga tccattaatt ctgcaaaagt aatatcatca aatccaataa tcgataattg
     3841 ttccatcact cgtttattct tattttttaa aaattttact aatttagccg ctaaataatc
     3901 tccagtaaca acaattgcag taaattcagc taattcttga taaattcgct caaattcaga
     3961 gttgttatat atccaatgcg atttaaaggt taaatgatat ttctttaatg tatcgatata
     4021 agcttgatgt cgttcttgaa taacacttgg ttcacctaca atgtagtcaa aagaaagaaa
     4081 cgctattttc tcatgaccat aatgaatcaa atgttccact gccaatgtac tagcttggta
     4141 atcatttgta tttacaaata aacgattcat ttgttgttca agttctctta atgtctcttt
     4201 tgaaagatgc gtatcaatat aaacaacagg accatcaaaa acgttattta attccaaaaa
     4261 ttcttttaca cgtacaccaa caacaatgaa cccctcaaaa cgccaatttc tctgaatagt
     4321 tttaacatct tctgctgaat taacattatg caccaataca aaataaccat gattctttgc
     4381 ctgttcctca attccagaca aaacttctgc ggtaaatgga tcagaaaata caaaatcagc
     4441 attatcaata gccgaaaaat aaagcaatcc tattaaaaat gcaatcgtct taccgattcc
     4501 ctgacagaca gaacacattc cactaggatc attaaatgaa taagcatttg catatcctaa
     4561 actaggtgtt gcaaaacatg aaaacaacac acgaaataac gcattgatgt tagtaatcgt
     4621 ccctaacgtt gagcgcgcac tgccaccaat cttcttttga ttgaccacaa tcgccgtaga
     4681 aagattatca atctcatcaa cctctggacg actgtattta ggtaaaaata aacgggtaaa
     4741 actatcatag gtgtcattca gttgacgacc agcttcttgg gtaatagtat caaaaacaat
     4801 cgatgatttt cctgaaccag atacgctggt aaaaatacta attttatttt tgggaatttt
     4861 gagactgaca tttttaagtt gtttctcttg cgtggataat ttcaatatac tcgttcattt
     4921 ggtactcccc tttattttga aatagttgag aatgtttcaa tgagacacgg tatttttggc
     4981 agatgtttag tttacctaga aagaggtata aaaatcaaga aaaaaagaga ggctgactca
     5041 taagtagttt tattagtcga aaatgatagt ttataaaaat aggaaccgca tcaaatcaac
     5101 attctttaga atggatttga tgcgtttttc tttatttttt gacttatgag tcaacctctt
     5161 actcttcgtc atggatttga ttagctaatt attgctccac ttgttcacga atgaaattta
     5221 agtactgcgg taaaaataaa ggaatttctt caaagcgtgg actttgattt tcatacgctt
     5281 ttgcatctag gtttccttcc cctgtagcaa tttcaatcaa taattgattt gggtcacgat
     5341 aataaagtga tacaaaaaat tcacgatctt ttattcctga atttataaaa ttgcgctctt
     5401 gtaattgttt gtcgatgcgt tgtaaatcag atttattttc tacgccaaac gcaacatgat
     5461 ggacgctacc aatcccttca atggcaactg gattctcacg atcttgaata atctgaactt
     5521 cttgatagaa ctgagtgtct tgattttcta agattgtcac tcgttgatga cccgaaaaga
     5581 aactgacgta ttctttgtgc taccagccta ccgacaataa ctctcgttca gtcgcatcgg
     5641 aatattgtac gcgtagttga atggcatcaa ttcctaaaat ggcatgctct gtagggatct
     5701 ggtctgtgac atacgggaaa taatcatcaa tatcttcaaa ttcgcgtaag ggaactaaag
     5761 ctaatcgtgt tgcatctggc ccttcaaaac gtaagatagg tcgtccagaa aatgattcga
     5821 ttccataatg gcaaacgcct tgtgtttcaa aatatatttc ccaaaaatgc aatgatgctt
     5881 cggaaggaac ttttagtatc gttcgttcaa tcgtattggt tccaaaaaac tggttgttcc
     5941 cttcttgtac ttcaaaaaaa gtcagttcag ttcccacacg gtggtgatta tctgaaaaaa
     6001 acaggtgata catggtatga tcgtcttgat taattgtttt cattagtaat tttaaaccga
     6061 gtatattatg ataaaagtca aatgtcggtc gaataaagcg attcacaata gaaatgtgat
     6121 gaatacgtgc gggcataata tctaaaaatg agaatcaaac ctataaaaga tggtgaacaa
     6181 cttttgtttt catcagaaga aaataagccc ctttatttag actatgtaaa tcacaatttg
     6241 aaaatcatca ttaaagagaa taacttaaaa catatcacac ttcatggttt tcgtcatacc
     6301 cattgtagtc tgctctttga atctggtgcg tctgtcaaag aagtgtatgt tcgacttgga
     6361 catacagata ttaaaaccac tatggatgat atatacaccc atatcgcgaa aaagcagaca
     6421 gaggaaacgg caattcgttt tgcagatttc atgagttcta cagaggaaaa gcaaggaagt
     6481 ttgcagaagg tcaaaagtat ggtcaaaaac gaaaaaagca gactatccct attagatagt
     6541 ctgctaaacg ttgagttatc agtatttttc ttattccgca gcgtttgcgt ttttgagacc
     6601 gtattgattc tcttttttta atttcatcct tttccaacct aaaaatgtta tttaattact
     6661 tagtttatat aacacgaacc tataccaagt accagtaacc ccagctttta ttccgcgaac
     6721 tttatgaaat aaaatcgttc tagataaaaa tagcttttaa tctttgtctt tactgtttat
     6781 aaaaattttt aaattataca cctagtttaa atccacgaag tgatgctcct atttcatctt
     6841 cagttatacc aatgtacctt tttgtaattg cttgcgagct atgattaaaa ataaacatta
     6901 aggtagcaat gtctctagtt ttcttgtagt agtgataccc aaaagttttt cttaatgtat
     6961 gtgttcctat atcatctcgt cctaattttt ctgctatatc tgcatatatc tgatagacac
     7021 gatcccttgt tatatgtcga ccttgttgcc gactcggaaa taaccaatca ttttcttctt
     7081 taccttcagt atataataaa atttcttctc tcaaattatc tataaaaatc ggttttcgct
     7141 tacctgtttt atgttcaaca ataataggct tacttttgcc tcgtatatct ccaactttta
     7201 atcctataat atctgacata cgtaacccat tattgattcc aaacaaaaat aaaaaactat
     7261 ctctttccgg attacttgtt cttcttagcc aaaactgaaa atcttgtatc tcttcatttg
     7321 tccttaacgg ttgtacatta tagctcacta ttgtttccct tcttaaaaat attctgagtt
     7381 aatgattatg tatat
//
LOCUS       DARBER010000120         7330 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DARBER010000120 DARBER010000000
VERSION     DARBER010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.124"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            348..617
                     /gene="rpsN"
                     /locus_tag="SCK06_002324"
     CDS             348..617
                     /gene="rpsN"
                     /locus_tag="SCK06_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1152284.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            632..811
                     /gene="rpmF"
                     /locus_tag="SCK06_002325"
     CDS             632..811
                     /gene="rpmF"
                     /locus_tag="SCK06_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1152285.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            843..1220
                     /locus_tag="SCK06_002326"
     CDS             843..1220
                     /locus_tag="SCK06_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152286.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1241..1390
                     /gene="rpmG"
                     /locus_tag="SCK06_002327"
     CDS             1241..1390
                     /gene="rpmG"
                     /locus_tag="SCK06_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1152287.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1414..2397
                     /locus_tag="SCK06_002328"
     CDS             1414..2397
                     /locus_tag="SCK06_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1152288.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2427..2549
                     /locus_tag="SCK06_002329"
     CDS             2427..2549
                     /locus_tag="SCK06_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1152289.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2567..4090
                     /locus_tag="SCK06_002330"
     CDS             2567..4090
                     /locus_tag="SCK06_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1152290.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4114..4353
                     /locus_tag="SCK06_002331"
     CDS             4114..4353
                     /locus_tag="SCK06_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152291.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4546..5181)
                     /locus_tag="SCK06_002332"
     CDS             complement(4546..5181)
                     /locus_tag="SCK06_002332"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1152292.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5202..5420)
                     /locus_tag="SCK06_002333"
     CDS             complement(5202..5420)
                     /locus_tag="SCK06_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152293.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5546..6750
                     /locus_tag="SCK06_002334"
                     /pseudo
     CDS             5546..6750
                     /locus_tag="SCK06_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            6745..7152
                     /locus_tag="SCK06_002335"
     CDS             6745..7152
                     /locus_tag="SCK06_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1152294.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2590 a         1089 c         1437 g         2214 t
ORIGIN      
        1 attggcgata aaccagttaa acataaaaga ttgaataaaa agttatagtt tctttcatat
       61 tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata
      121 aaagtgaatt cttatttttc agagcttaaa ctgcatattc ttttaaatga tttgattatc
      181 tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg
      241 ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga
      301 ttacgatttg agatgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt
      361 cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtacgca gataaacgtc
      421 aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc
      481 cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt
      541 tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa
      601 aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa
      661 ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga
      721 cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg
      781 aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca
      841 aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga
      901 cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta
      961 ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg
     1021 aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg
     1081 acgaggagtt atttcaatgt attgatggtt attgggagat acctgttgat atgaaagaaa
     1141 atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag
     1201 catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt
     1261 ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc
     1321 tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa ttttcaaaga
     1381 agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct
     1441 gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac
     1501 cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa
     1561 ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt
     1621 cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa
     1681 caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt
     1741 caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact
     1801 gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt
     1861 gtcatagcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa
     1921 aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat
     1981 agtgaaacga ttcaaaaaaa ggatttttat aatttggaaa aattcaatca tcccttagtt
     2041 tttgaaagtg attcatcaga aaatcactat aatggcaaac actatcatca acatcatcaa
     2101 gaacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa agatttttta
     2161 ttccaattaa ttgaccggct gattttaacc aaccaaggga gcctgtatcg ttttaaagga
     2221 ttgattgctt tgcaagatca agattttata gttgccatac aaggggtaaa cgaacaggtt
     2281 aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat tggaaaaatg
     2341 ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag caaataatga
     2401 taagtattga ttaggggtaa tttctaatgg agaaaacgga tatatctagt gcatatcgac
     2461 gacttaaaag cccgaatatc aaaactagaa aaagggcatt aaaaattatc aaagaacata
     2521 aacaaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga agaaaatcag
     2581 tgtaggactt attgggatag cagctctagg attattggga gcttgctcca gcactaatga
     2641 tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat tctatccaat
     2701 gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa tggttccagc
     2761 gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag ctcatgatgc
     2821 tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag
     2881 ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt tattgccagg
     2941 tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac tagatcctca
     3001 tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg atcagttagt
     3061 taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt atttgactaa
     3121 actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac aaaaaagttt
     3181 tgtgacacaa catgcagcct ttggatattt agctttggat tatggattga tacaagttcc
     3241 tattgctgga ctaagtcctg aagaagagcc atcttctggt cgattagctg aattaaaaga
     3301 gtatgttaaa aaaaataaga tcaattatat ttactttgaa aagaatgcta atgataaaat
     3361 cgcaaaaact ttggccaatg aagctgggat aaaattggaa gtattgaatc ctcttgaaag
     3421 tcttacaaaa gaacaaatgg ataatggtga agattatgtt tcagtaatgg aagacaatct
     3481 aaaagcgtta gagaaaacaa ctatggttgc tggtgaagag gtagttccag aaaaagaagc
     3541 gaaggatgaa aagacagttg ctaatgggta ctttaaagat gccgatgtga aggatcgcga
     3601 attgagtgat tatacaggcg aatggcaatc tgtttatccg ttgttaaaag atggaatttt
     3661 ggatgaagtt ttcgattaca aagcaaaact caataaagac atgacagctg ccgagtacaa
     3721 agattattat actactggct ataagacaga tattgatact atcaacatta aagacaacac
     3781 cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg ttggatacaa
     3841 aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc
     3901 aggtgctggt cgttttaaat atattcaatt tagcgatcat ggaattgcac caagtaaggc
     3961 agagcatttc catattttct ttggtggaga aagtcaagaa aaactttata atgaaatgca
     4021 taattggcct actttctatc cagcttcatt atctgagcat gagattgctc aagaaatgat
     4081 ggcccattaa aatccaatct ttggaggaaa tacatggtaa gaaaagtatt attgtctatg
     4141 gattccaaga atattgatgc ttatcggcgt tatcaatttg caaagcaaat gagattaaat
     4201 gggagtacat tagctgatat tgctgcaact ttacatgtta gtattgacac agttcataga
     4261 gtattaaaac gggatcctga ttgttgtttt atcaaggtca atactggaag aagtagacgt
     4321 ttagcaaaac ggcataatag cttggccaat taattgataa taccagagta gatcattttt
     4381 cccttatttg gatttgatct gctttttatt atttcttgaa ttgtcaaatc aagcgcaaca
     4441 ctttatcaaa gaagacttca taaggtgttc tccaatctaa acactttcta ggtctgttat
     4501 tcagtttttc aatcatctga tttatcgttg agttatctac cgtagctatc tctttcgatt
     4561 ttggcaagta ttctcgaatg agtctattcg tattttaatt tgttcattgt tgccaagggc
     4621 atgaggatct ggaaaataaa agggcatatt attcaatact tctgtaactg cgaaatgatt
     4681 ggagaattct tttcctctat ctggagtgat cgttttgacc gcctcatttg gaatactgct
     4741 taaaagttca atcattttct cgctcacata agcggctttc tttctgggga ccttctttcc
     4801 tgctaaaagg aagcatgatt ttctgtctgt caaagtcact aaacaagtac cactagtttt
     4861 gccagctaca atatcggctt tccaatgacc aaaatcactt ctatcatctg cggattttgg
     4921 atggccatgg atcgaatgag taaggcaaat cttcccacga gactcgacat gattttttgt
     4981 atgtcttgtt tttcctcgat ggtgcaatga acggatcagg cctttattgc cgtgtgagag
     5041 gggttctgta tcgaaaagat cattgcaaat tgcacggtag atcgtgataa aactaattga
     5101 aaagtccata tgttcatgct tcaaacgatt cgagattttc tctggcgacc actgaaaatc
     5161 gacaaataaa tgacgaacaa ttgaccaaac tcacggatca tttagaatgt gataacgtcc
     5221 gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgatt agtttttttc
     5281 ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc gttgccaatt
     5341 atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttt gagtatagta
     5401 agatgacaat actggctcat aattattttc cttctaggag tgttctcgac aaacttatac
     5461 tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta aattcaaggt
     5521 atagaaagag gttccctatg aaaaaatacc tattacaatc ttaactggct atcttggttc
     5581 aggaaaaacg atacttctaa atcatattct gtacggtgat cacggaaagg aaattgctgt
     5641 cattgtaaat gacatagggg aagtaaatgt tgatggggta ttagtcgaca agaaaggttt
     5701 taaaagaact ggagaaaaac ttgtttcgat gccgaatgga tgcatttgtt gtacattacg
     5761 cgaagatttg attgttgaac tagatcaatt agtgcaattt tctgacttga atcaaattgt
     5821 tattgaagct tcaggaatca gcaaaccaat tccaattatt caagcaatta tattagctaa
     5881 aaacccttta gacattgatt tgacccaacg attagagatt ggtgccatta ttatagtagt
     5941 ggatgtaaaa cgtttttggg ataatttcga aacgggtgaa aagattcagg aaagaaaagc
     6001 tgaaggtaat gtagttgaag aagaacagga tattcgtggc ttgttaattg atcaattcga
     6061 gtgttgtaac atattacttt taaataatag tgacttagtg tcaaccaaga cgatttacaa
     6121 aattaacggt ctaattgcta agttacagtc caatgccaaa ataattagga caattaaagg
     6181 tgaagtagac ttatcgaaaa ttctaaatac aaatcttttt aatatagatg agacttccta
     6241 ttcaatgggc tggttcaaag agttagagct tggctatcaa aatcatattc ctgaaacaga
     6301 agaatatggt atctcaagtt ttatctatcg aaatcggtta ctccttgacg cacaaaaatt
     6361 tgcccatctt acggataaaa attttcctgg caatattaca agagcaaaag ggtttctttg
     6421 gtttgcccaa catagtgatt tttcgacgat ctttgaaaca tcgggtagcc ataaaagtat
     6481 tgaatcatta aattattggg tagctagttt acctaaaatt gagtgtcaaa tgattataca
     6541 aaaaaacccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
     6601 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
     6661 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaattct
     6721 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
     6781 gatgtcataa ttattggcat tttgattatt ccctctttta ttcccttatt tgtattctct
     6841 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcaaattgat
     6901 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
     6961 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
     7021 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
     7081 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag caaagattac
     7141 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
     7201 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
     7261 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
     7321 ttacttattt
//
LOCUS       DARBER010000121         7315 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DARBER010000121 DARBER010000000
VERSION     DARBER010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.125"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(70..690)
                     /locus_tag="SCK06_002336"
     CDS             complement(70..690)
                     /locus_tag="SCK06_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG1152295.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(766..1203)
                     /locus_tag="SCK06_002337"
     CDS             complement(766..1203)
                     /locus_tag="SCK06_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG1152296.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1366..2196
                     /locus_tag="SCK06_002338"
     CDS             1366..2196
                     /locus_tag="SCK06_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG1152297.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2180..3547
                     /locus_tag="SCK06_002339"
     CDS             2180..3547
                     /locus_tag="SCK06_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1152298.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3566..4375
                     /gene="yidA"
                     /locus_tag="SCK06_002340"
     CDS             3566..4375
                     /gene="yidA"
                     /locus_tag="SCK06_002340"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG1152299.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4418..4861)
                     /locus_tag="SCK06_002341"
     CDS             complement(4418..4861)
                     /locus_tag="SCK06_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG1152300.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4995..5366
                     /locus_tag="SCK06_002342"
     CDS             4995..5366
                     /locus_tag="SCK06_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1152301.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5535..6317)
                     /locus_tag="SCK06_002343"
     CDS             complement(5535..6317)
                     /locus_tag="SCK06_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG1152302.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6314..7285)
                     /locus_tag="SCK06_002344"
     CDS             complement(6314..7285)
                     /locus_tag="SCK06_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG1152303.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
BASE COUNT         2220 a         1528 c         1265 g         2302 t
ORIGIN      
        1 atcgtgtcac acctccttat tctgataggc tgatcgtctc tgtcacaacc tctcgtaaga
       61 aaataatgtt tattcttttt cagagtcaga aaattcttcc tcttcataat cttcatcatc
      121 atcgtcttcg tctatgatac ttaaatcttc ttcgatattt cctggcaagt cttcttcatc
      181 atctgtcggg tcatctgctc cgatttcttg caagtcagaa ttataagcag cgatttcttc
      241 atcttcatca tcatcgtcat caaacagatc atcgtcatct tcttctgtca gatctgtatc
      301 ttctgggtca tcgtcattgt aatcgatcgc gtcttcatca tcattgataa aggcgttgac
      361 tttcttgcgt ttacggcgac gtggagtatc ttcatcctct tcgtccattc catggtttac
      421 ttcttcatcg atcgaatcga tagcatacca tgaacgcaag ccccaacgat tatcccctaa
      481 tgagataaag ctgccgtcaa tattcaagtc agtataaaat tgtgcgagtg aatcacgaat
      541 ttcactgtct gatttcccaa gatagttttg gatctggtta gctaaatcag aaaaatccat
      601 gacatcgcca cgttgttcta agatcgcgtg tgctacttcg atcattgata attcgctttt
      661 gtttgcacct tcaaatacac taatttccaa tcaggacacg tcctttcaca gtctactcct
      721 tatcatacaa aatcagacac aaaaaatcaa acttttcttt tatatttacg ctgtaaaatc
      781 caaggtcaaa taatattcgc cgattttttc cggtcccatg aacagatcat aatcgattcg
      841 aaccttccct cctgtgggac ggtctttcaa actaacatgc agttctttcg taaaagtagc
      901 aatctggatc ataccgtatg gtgttcgata ggatgtttct aaccgttctt tatagccaaa
      961 tttcaaacgc atccgcattt ctcctgcacg gatcagctgt acatgaccat ctggaactaa
     1021 tttgatcgtt acaggaatct catcgcccgt ctcctcctga atctctttat aacgtatgta
     1081 cagcgtatcg ccaattgtca ccatttgccc tttcaaatca aaataaaaat cttgcacttc
     1141 cccttgctgt tttacttttg tacgcaattt gattgaaatc ggtgttcctt gtgataattc
     1201 cattattcat tctcctttac ttctcatagc tacattgttc ctagtttctt ttgttttgtc
     1261 aaccaacttg tccattttta cagaaaacct tttttccaaa acaactcttt gattttagag
     1321 tataatattg tagaaaatat gaatttatac aaaggagccg gttgaatgaa ccctgcgatt
     1381 cgactagatc agcacattta tcaaaaaaac gacttttccc cttttgcatt aacagatata
     1441 ttaacggaat attctaaaga acaccagcaa atctttttac atttctggca atatgactgc
     1501 acagtcatct taggtatgaa ggatgttcga actccttttc taagtgacgg gatccaaaca
     1561 ctgaaagatg caggttacgc tccagtgatc agaaattcag gtggattagg gatcgtatca
     1621 gatgaaggaa ttttgaatat ttctttgatt ttcccccaaa gtgacacaaa aaaaataacc
     1681 atagatgacg cttacgagca aatgctccta ttgactaaat acgcttttcc aaaggcaccg
     1741 atacaagcat tcgaaatcac ccattcttat tgtcctggaa cttatgattt aagcatccat
     1801 ggcaaaaaat ttgccggtat cgctcagcgt cggatcaaaa atgggatttc tgttatgatg
     1861 tacttaagtg tcaacggtaa tcaggaaaaa cgcgggcaag tcatgaagcg attttatcaa
     1921 tctgctttaa aagaagactt tggtacaaat ggctatcctg cagtggatcc tgctacaatg
     1981 gcaacacttg aagaactgct tgaccaaccc ctttttgtaa aagaagtcaa agaagcattt
     2041 gcaaaagctt ttgaaaagct gtattctgca agtgatttac aggattctac cgactggcta
     2101 aaaaaagaaa tccagctgga gcaatggaac aaccaaatcc agagaatgga acaacgtaat
     2161 aacataaagg agctacatta tgacaacccc ttataaatat caaatgctgc ctatggaaaa
     2221 agtttttcgt gatccagtcc ataactatat ccatgtccag catcaagtaa tcttagattt
     2281 gatcaattcg aaagaagtcc aacgtctacg acgcattaaa caattaggca cttcttcttt
     2341 cacttttcac ggagcagaac atagccgttt tactcattca ttaggcgttt atgagatttc
     2401 aagacgaatc tgcgatatct tttctcgaaa tttttcaaaa gaaaaaatag gcaacggagg
     2461 ctgggatgat caggaacggc tggtgacttt atgtgcggca ctgttgcatg atgtcggtca
     2521 tggtccttac tctcatacat ttgaacatat cttccatacg aatcatgaag ccatcactgt
     2581 agagatcatc acttctccag aaacagaagt gtatcagatt cttaaccgag tagaagaagg
     2641 ttttcctgaa aaggtagcta gtgtgatcca aaaaacttac cccaacccac aagttgtcca
     2701 aatgatttct agtcagatcg atgcagatcg aatggactat ttgcttcggg atgcttattt
     2761 cactggaaca gaatatggaa catttgatct tacccgaata ttacgtgtga ttcgtcctta
     2821 tgaaggcggg atcgcttttt ccatgagtgg catgcatgca gtggaagatt atatcgttag
     2881 ccgatatcaa atgtatgtgc aagtgtactt ccatcctgtt tcccgtggta tggaagtaat
     2941 tttggagcat ctgcttcatc gcgcacacga gttgtatcaa gaagatgcca gcagttttga
     3001 actccattct caattacttc tgccatttct tgacggaagc tttactttag aagaatacct
     3061 caaacttgat gatggggtct tgggcactta ctttactcaa tggtctgaat ctgctgatcc
     3121 tattttaaat gatttagcta aacgtttttt gaatcgtaaa ccactgaaat ctgctacttt
     3181 ttctggcaac cgggattcaa aattagtcca agaactcact ttgttagtag aaaaggtcgg
     3241 atataatcct gtttattaca ctgctgtgaa ttctagctac gatctacctt atgattttta
     3301 tcgcccagaa caaggcagac atcgcacaca aatcgagatt ttgcgaaatg acggaacttt
     3361 gatagaatta tcacaggtta gtcagcttgt tgctgcatta gcgggacaag aacaaggaga
     3421 tgagcgattt ttctttccaa aagaaatggt tgatccgtcc ttgcgtgatc attatgattt
     3481 atttgacgaa acttatcaag aatttgccag ccatatccgt aatggcgcat tgattgaaat
     3541 aaattaaaat atattggagg aaattatgtc tatcaaacta gtcgctatcg acatcgatgg
     3601 tactttatta aatgagaaaa gagaagtaac aaaagaagta aaagaagcga ttgccgctgc
     3661 tgttgcaaaa ggtgtttcga tcgttttatg cactggccgt cccttaccag gtgtccagga
     3721 acagttaaac gagttgaacc tttttcaaga taatgattac gttattactt ataacggggc
     3781 actcgtacaa caaacgaaat caggtaaaat catcgcccgt cacggactga cacatgaaga
     3841 tttcctagag atcgaagtga tggcacgccg tgtcggcagt catctccatt cgatcgatga
     3901 caaagcgatc tacacgccta atcgagatat cagcgcttat acgatccacg aagcaagttt
     3961 agtcaagatg ccgctaaaat atcgaacacc tgaagaaatg acaccagaca tgaacatcgt
     4021 caaaatgatg atgatcgacg aaccagaaat tttagatgca gcaattgcgc gtttaccgca
     4081 aactttccgt gataaataca caacggtcaa aagtgcgccc tattacttcg aagtactaaa
     4141 taaagaagca agtaaaggtg cggcagttgc gaatctcgca cagcaccttg gtattgatca
     4201 agatgaaatc atggctatcg gagataacga gaacgactta tccatgattg aatatgctgg
     4261 tctaggagta gcaatgggaa atgccgtttc tcttgtcaaa gaagcagcaa atgtcatcac
     4321 ttcatcaaac gacgagcacg gtgtagctga agcaatcaaa aaatatgtac tgtaataaat
     4381 aaaaaaactg agagaaagct gatctactcg ctttctttca gtttttattt ttttcgatcc
     4441 atctctcgac tgcccatcgg tggctaccac gtttcaattt cacaagcgct tcttgagggc
     4501 tcacccacca aattttattg gtcttttcag ttggttctcc tatacgcttc caagaatcag
     4561 caacaaagaa atatcctgga ttataataat aagttgcacg ataattcgag taaaaatact
     4621 catcagcttc tcccagataa gaagaaattt ctgccacaat tcccatttct tctatcagct
     4681 cacggacaat cgcttcttct ttcgtctctt ctccttcaat ttccccgcca ggaagaaaaa
     4741 aagcaccatt tggagcctcg attactgcaa tctgctgatc tctttctatg acaatatatg
     4801 ctgcataacg ggtttgataa tcagcaaatt gctttttttc accaaaaatt ggtttatcca
     4861 tgaatagttt ccaccttaac agttttattt tctatccatt ttacatgaga aatttctcat
     4921 gaacaagcac aaatcaacta tttccttact tctttttgtt atgattgagt aaaaggaaaa
     4981 ggagagatga atctatgaaa atggctcaca catgtgttcg agtgaaagat cttgaagcat
     5041 caatcgaatt ttatcaaaag gcatttggtt ttgaagaaag ccgccgccgt gattttccag
     5101 aaaacaaatt tacacttgtt tatttgacac ttcctgggga tgactatgaa ttagagttga
     5161 cttacaacta tgatcatggt gcgtatgatc taggagacgg ctatggtcat atcgctatag
     5221 cagctgatga tctagaaaaa ttacacgagc aacataaagc tgccggtctt gagattactg
     5281 acttaaaagg attacctggc actgcgcctt cttactactt tgtcattgac cctgatggtt
     5341 ataaaatcga agtcatccga ggatagttag tatggggcat tttactcttg attgaataaa
     5401 aaacaaacac caataattaa aatgagacgc aaacttttca ttaaattgaa aaagtttgcg
     5461 tcttttttta acaaccattg tagctataag gttgccactc tccaccaatt tatttttaat
     5521 tcatttgaaa gaaatcaaat aaacgaggta cctttagaaa acaaaactgc ttttcctcct
     5581 attatcacac gatcttcttt taattcacat tctagatccc cgccccttga tgacatttga
     5641 taagctgaca attttgtttt attcattctt tctgcccaat aaggaataag attagaatgt
     5701 gctgatccac atactggatc ttcattgatt gttaattttg gaaagaaggt tctagaaaca
     5761 aagtcttttt gcacactttc tgctgtcacg atgactccta ttcctaattc aaaatttttg
     5821 atagctgtaa aatctggctg caatttagcc accgtttttt catcttctaa tacgaaaaat
     5881 aaatcacgtg ctaagtaggc ttctttaatc ttagccccaa tcgcttcttc atattcagac
     5941 aatatcggca cttttttcgg cataatacta ggaaaatcca tgtaataata atctttaatt
     6001 cttgtcacaa atagattacc gctttgagta gaaaatttaa tcgtttcatc aggtattttg
     6061 taatagttaa ataaaacaaa tgctgttgct aatgtcgcgt gaccacaaag atcgatttct
     6121 cgatctggcg taaaccatct aagctcaaat tcttggtttt tcttcactgt aaatgctgtt
     6181 tcagataaat tgttttcaat ggcaattttt tgcatcgtac cttctggcag ccattcttct
     6241 agcacataaa ccgctgctgg atttcctttg aacacctcat ctgcaaatgc atcaactacg
     6301 taataatcta agttcatttt aaagcccctt tctctaattt gttataaata tattctgcac
     6361 aacaaagatc ttcaatagct agaccaactg cgtcgaaaat cgtcggcctc gattctcctt
     6421 tataaaatgt ttttttctca gaaactaatt cgcctaatgt accaacaata caagattcct
     6481 ctaaatctcc ttctgccatg gctataagca ggtccccgct ttctgttaag gcagctgagt
     6541 aatcatctac atagatttga gaagtttcca tcaacgatgt acttatctct cttgtatcag
     6601 gagtaaatgt gcccacagca ttgatatgtg cgtcttccct tacccactct ttttgaagaa
     6661 atgcctcttt actagaggtc aacgtacaaa tgatttccgc tggacgtact gcatcttcta
     6721 ctgatattgc atttataaac gttattttag gatactgttt tttagcttta gcgataaaag
     6781 tatttcgtct ttcttctaaa atatcgtaaa caaacactgt agtgatggga cgaatggtag
     6841 aaatcgcagt caaatgcgac aatgcttgtt gaccagctcc aattaaagca agtgtttctg
     6901 catgcggatt tgctaagtaa tctgttgcca gcgcagatac agcagctgtt cgaatccagg
     6961 tgactgcatt tgcgtttatc aatgctttgg gtagtccgtt ttgagcatca aacaagaaaa
     7021 tctctcctaa atgagaaggt aaatgatact gatgattatc cggaaaagcg gtaatgactt
     7081 ttgtgccaaa atatctgttc tcccccaaat aagcaggcat catcgcaaac atatttttgc
     7141 cttttccgtc aggtaaaatt tccaccaatc gaggattttg gctgatttta ccactttcat
     7201 aatcaaaaaa acattttttc attacttgaa tcgcttcttc aaaactaaaa gaagctacta
     7261 cttcctcaaa attaataatt ttcatagaca attccctcct cacaaaagat taact
//
LOCUS       DARBER010000122         7262 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARBER010000122 DARBER010000000
VERSION     DARBER010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.126"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..2656
                     /gene="clpB"
                     /locus_tag="SCK06_002345"
     CDS             47..2656
                     /gene="clpB"
                     /locus_tag="SCK06_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG1152304.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2807..3493)
                     /locus_tag="SCK06_002346"
     CDS             complement(2807..3493)
                     /locus_tag="SCK06_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152305.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3471..3965)
                     /locus_tag="SCK06_002347"
     CDS             complement(3471..3965)
                     /locus_tag="SCK06_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1152306.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(3985..5205)
                     /locus_tag="SCK06_002348"
     CDS             complement(3985..5205)
                     /locus_tag="SCK06_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1152307.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5352..7187
                     /gene="lepA"
                     /locus_tag="SCK06_002349"
     CDS             5352..7187
                     /gene="lepA"
                     /locus_tag="SCK06_002349"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG1152308.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2385 a         1393 c         1494 g         1990 t
ORIGIN      
        1 tcaaagaaat aagtgatctt acctttaatt aggagggtat acctgtatga atattgagaa
       61 aatgactacg acgcttcaag aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa
      121 acatcaagat atcgacattg cccatgtatg gaaaatcttt ttacaatcta atcattttgg
      181 tcgaaatttc tatacggatg ccggattgga tgtcgaatct tttgaacatg aaattgatcg
      241 tctactggat gagtatccag ttgtttctgg tggaaacgtc caatatggac aaaacctcag
      301 ccagaatttc tttcgattat tgaatgaagc agaccaaata cgtgaaagct tcggcgacga
      361 gtttctttca acggaagtcg ttattttagc gttgatgaaa ttaaaaaatt atccgttaac
      421 tgtttatttg aataaaaacg gattatctga aaaagaattg cgtaaaaaca tcgaagagat
      481 gagaggagga gaaagagtga cctcacaaaa ccaagaagaa caatacaaag cattagaaaa
      541 atacggcgtt gatttagtac agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg
      601 tgatgaagaa atccgtgatg tcatccgtat tctttcacga aaaacaaaaa ataatcctgt
      661 tctgatagga gaacccggtg tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat
      721 cgtacgcaaa gatgtgcctg aaaatttaaa agataaaacg attttttcat tagatatggg
      781 tgcattgatc gctggagcaa aattccgtgg tgaatttgaa gaaagactaa aagcagtctt
      841 gaaagaagtc aaaaaaagtg atggacgtat cctcttgttc atcgatgaaa tccacaatat
      901 cgtaggggca ggaaaaacag aaggaagtat ggatgccgga aatctgttga aaccaatgct
      961 tgctcgcggt gaattacatt tgatcggtgc cacaacttta gatgagtacc gtcaatatat
     1021 ggaaaaagac aaagcgttgg aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt
     1081 ggaagatacg atcagtattc tgcgtggatt aaaagaacgt tttgagatcc accatggtgt
     1141 taacatccat gacaacgcat tagtagcagc agctacttta tctgaccgtt atattactga
     1201 tcggttctta ccggacaaag caatcgattt gatcgatgaa gccagtgcaa cgattcgggt
     1261 agaaatgaac tctatgccaa cggaattaga tcaagttact agacgtttga tgcagttgga
     1321 aatcgaagaa gctgcactga aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa
     1381 tttgcaagaa gaattagctg aacttcgtga agaagcaaat gcgatgaaga tgcagtggga
     1441 aacagaaaaa gaagaagtca attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa
     1501 acacgaatta gaagatgcag aaaataacta tgatttagaa cgcgctgctg tattgcgtca
     1561 tggtacgata cctcaattag aaaaagaatt gaaggaatta gaagctaaag caaaagacag
     1621 cgagattaag atggttcaag agtccgttac cgaaaatgag attgctcaag tcgttggcag
     1681 attaacgggt atccccgtaa cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt
     1741 gaatgaaaca cttcataaac gagtgattgg tcaagatgaa gcagttgatg cggtcagtga
     1801 cgcggtaatt cgttcacggg ctggattgca agacccaaat cgtcctctag gttctttcct
     1861 tttcctagga ccaactggtg tcggaaaaac cgaattagcg aaagcgttag ctgagaactt
     1921 gtttgactca gaagatcata tggtacggat cgatatgagt gaatatatgg aaaaacatgc
     1981 tgtgtctcgt ctagtcggag ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt
     2041 aacggaagct gtcagacgaa atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc
     2101 gcatcccgat gtcttcaata tcttgttaca agtcctagat gacggacgct tgactgattc
     2161 aaaagggcga gtagtagatt tcaaaaatac ggttctgatc atgaccagca atatcggttc
     2221 acagttactg ctggaaggcg tgactgctga tggaacgatt ccagaagcag tggctgaaca
     2281 agtcaacacg ctattacgtg gaaacttcaa accagaattc ttgaaccgaa tcgacgacac
     2341 gattttattt acaccattga gcctagacaa cgtgaaagga atcgttgaca aaatggtggc
     2401 gcaattggca caacgtctgg aacatcaaga aatcctgctg acaatcagtg atgaagccaa
     2461 aacgtggatt gctgaaaatg cttatgaacc agcatatggt gcaagaccat tgaaacgatt
     2521 tatcactaag gaagtagaaa caccactagc taaagaaatc gttgcaggac atgttatgcc
     2581 taaatcaaaa gtaacgatta ctttacttga tggacaactt catttcaaaa cagaagagct
     2641 agaagaaatc gtctaatctt ccattgagac tataaaagaa aaaaggggtc tgacacaact
     2701 agtgtcagac ctcctttttt caaataaaca gtgttccaga agtagtttct tcgaaaaata
     2761 agccgaaatt tcttgaagct gcccgcttct gtcacaacct cttttattag gtatttttca
     2821 ctttttctaa tttataactt tcaaagtaac gctcatcttt tttactttga gaccatgcaa
     2881 acaatcccat taaggcagca cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca
     2941 atgctgctcg tccgacaaac agtgtgccat cagaagcagc aaaacgttga agattcatgc
     3001 ctaagaactg gtcgcataag aattcccaga actcccaaaa cacgccgcaa agtccagcaa
     3061 acgcaaagcc gaataagagg aacaaccaag gactggtttt tgatatctca gcatctttca
     3121 tcaagtaacc aatcaatcca tagccaagag ctgtcaggac cattggactc accgcatgta
     3181 agattttgtc ccaaaaagag atgatactga tcaaatgcat ccctgtgcct aaaaagacag
     3241 agatgaacaa gaaaaaccag taaaacaaaa cgattgctgc tggtatttta agtttgaaaa
     3301 ctttcaaaat gacttgtggc atgaaaatca atgcaatccc taataaagat tccacgataa
     3361 atgtgattcc ttgagaagtc gaatattttt gtgtgataaa ttcataaaaa ttatagcaga
     3421 caacgaatac accaaaagca agtaaagcta tttgcacctt tttgatttct ttaatcactg
     3481 aaagtccctc catctaaaaa tctttttact tctgaaagca actcaatttc tgaagaaacc
     3541 acttttccgt tcaatttgat caaacctatt gtatataagt tcaggtagtg aaattgattt
     3601 tcagctgttt cttgtagtgc agcgattttt ttcgggtggg ttccgccttg ctgtcgagta
     3661 tcagtatata agccgatcac tgggatgcct ttcgcataag ccacaccgat ttccgatgct
     3721 accccggcat cgatcgtcag cccatctagt aaagcaatca ttaaatcact agccaacact
     3781 ttttctgtgt cagcaagagc aatcattcga ctgtctgcat aagcagtttt gtcattgatt
     3841 gcttcatttt cttgaggaag atagatcgaa agctcagaag agagctctct gatttcttta
     3901 actaactgtt ggttatataa caaatcactt ttagcaaaca ttggtgcggc aaaataaatt
     3961 ttcatctaaa catcatcctt tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg
     4021 aatgatacgc tctagtgact tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc
     4081 caaataagaa gcacggcgat aattcatcat caagcaagtc aatgtttgga tatccagtcg
     4141 aacggatttt cctataggtt catttaagac acggacttgt ccctgttcat cccatgttaa
     4201 agcgaagaca ccattattcc aaggagcgac aggatcttca atgatgaaat gaaaagcatc
     4261 agctgttcca acaaacggaa accgttgaag gaattctttt acatcaacga tccgagccat
     4321 aaaatatggt tcgatctgct ctttgatctg actatcttca atcaaaaatg ctaaaggctc
     4381 atttttataa atatctccat gtacccagta gatcatggaa aaatgtgcgg aaataaagtt
     4441 ccataagcca tttcgagctt cttgatcaag atagaacatt tcttttacgt gaaatatttc
     4501 ttcagctacc cagtagaaca gaacacctct tggttcatgg ttgctatcgt agtagacagc
     4561 ggctgtgcgc tcatcttcat tttcgaaacg ccaatactct tcccaatgaa aatcactgcg
     4621 gaataaagca ccatggtttt gttgggcaaa tttgtcataa acggaaaata cgtctggatg
     4681 atcaactggt aatcgctcaa ccataccact tactgggatt tgtttaggta gctgtgtatc
     4741 tctgatttta aaggaaagtt tatcagacat gatttcccat cctttacgcc gatagtaggg
     4801 gatactgtaa ggatacaaat aagagatcca ttgcttattc ttgcgcatat tatccaatgc
     4861 aacgataatt agatcttgca tcaatccatg gttagcatat tctggataag ttccaactcc
     4921 agtcacacca cccattttat aacgtgcacc gtgaatattg acttcacaag gatagatagc
     4981 gatttgagag atcagtttat tttcgtggaa ccatccgaac actttcgata cttccaaaat
     5041 cggttttttg gaacggataa aagctcgttt attctcaaac ccgctttcct cgatgtctgc
     5101 ttcagtcact tggaacacat aggaaagcaa ttcattgaat tgatcgatat gttcttgagt
     5161 gaccggtttt aattcaagat tttctctaaa atctttttga tccatttttt catctccatt
     5221 tattagcttg ataacagtat agcaaaattc tacaaaaaaa acttctatag ctgactaatt
     5281 aaaaactctt taaattactg cttttcgttg gtataatgga aagtgctgat tatatgaaaa
     5341 gaggagacgc tatggatata aagaaaatga aagaacgcca agagcagatt cgtaattttt
     5401 cgatcatcgc tcatatcgac catgggaaat ctacattggc cgatcgtatt ttagaaatga
     5461 cgcacactgt gacgtctcgc gaaatgcaag atcagctgtt agactcgatg gatttggaac
     5521 gtgaacgtgg aatcacgatt aaattaaatg ctgtagaact caattacaca gcgaaaaatg
     5581 gagaaacata tatctttcac ttgattgaca ccccagggca cgtcgacttc acctatgaag
     5641 tctctcgaag ccttgctgcc tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa
     5701 tcgaagcaca aacattggcc aatgtttatt tagcgttaga caatgatttg gagatcctgc
     5761 cagtcatcaa taaaatcgac ctacccgcag cagatcccga acgtgtccgt caagaaatcg
     5821 aagacgtaat cggaatcgat gctagtgaag ctgtattagc cagtgccaaa tcagggattg
     5881 ggattgaaga tattctcgag cagatcgtgg aatatgttcc tgcaccaagt ggagacttag
     5941 atgcaccgct aaaagctttg atctttgact ctatttatga tagttaccgc ggtgttgttc
     6001 taaatgtccg gatcacggac ggtatggtca aacccggaga caaaatcaaa ctgatgagta
     6061 atggcaaaac atttgatgtt acagaggtcg gggtcttctc gccaaaagca gttgctagag
     6121 actttttgat ggtcggtgat gtcggttata tcacagcaag tatcaaaact gtccaagata
     6181 cacgagtagg ggatacagta acgttagcag acaatcctgc cgctgaagct ttaccaggtt
     6241 accgaaaaat gaatccgatg gtttattgtg gtctttatcc aattgatact tctcgttaca
     6301 acgatttgcg ggaagcttta gaaaaactgc aattgaatga tgctgcttta caattcgaac
     6361 cagaaacctc tcaagcatta ggttttgggt tccgctgtgg attcctcgga cttcttcata
     6421 tggatgtcgt ccaagaacgc ttggaacggg aattcaatct agaattgatc acgactgcac
     6481 catccgttat ttaccatgtt aataaaacgg atggtacgac tgctacagta gataacccgg
     6541 cagattttcc tgaacctgta acgattcagg atgtagaaga accattcgtc aaagcacaga
     6601 tcatggtccc aaatgatttt gtcggtgcag taatggaact ttctcaacga aaacggggag
     6661 aattcattac gatggattat ttagatgatt accgagtaaa tgtcgtctat aatattccgc
     6721 tttctgaaat tgtctttgac tttttcgaca aattgaaatc aagcacgaaa ggctatgcct
     6781 cattggatta tgaaatgtct ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc
     6841 tgaatggaga aaaagtcgat gcattaagct ttatcgttca ccgagacttt gcctatgaac
     6901 gtggaaaagc aatcgttgaa aaactgaaaa aactaatccc acgccaacaa tttgaggtgc
     6961 caatccaagc agctattgga caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta
     7021 aaaacgtctt ggctaaatgt tatggtggag atgtttctcg taaacgtaaa ctcttagaga
     7081 aacaaaaaga agggaagaag cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag
     7141 ccttcatggc cgttctgaaa atggacgagg acgaaccgaa gaaatagcaa gggtttgttt
     7201 gaaaacagtg taaaaaataa accatgttta catggatttg ccaccggttt gccaccggtg
     7261 gt
//
LOCUS       DARBER010000123         7239 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DARBER010000123 DARBER010000000
VERSION     DARBER010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.127"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..572
                     /locus_tag="SCK06_002350"
     CDS             <1..572
                     /locus_tag="SCK06_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152309.1"
                     /translation="GVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQ
                     VLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILK
                     SLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCR
                     GLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            755..3430
                     /locus_tag="SCK06_002351"
     CDS             755..3430
                     /locus_tag="SCK06_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG1152310.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3612..3851
                     /locus_tag="SCK06_002352"
     CDS             3612..3851
                     /locus_tag="SCK06_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152311.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3867..5240
                     /locus_tag="SCK06_002353"
     CDS             3867..5240
                     /locus_tag="SCK06_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1152312.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5244..6878
                     /locus_tag="SCK06_002354"
     CDS             5244..6878
                     /locus_tag="SCK06_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1152313.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7041..>7239
                     /locus_tag="SCK06_002355"
     CDS             7041..>7239
                     /locus_tag="SCK06_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152314.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2577 a         1131 c         1441 g         2090 t
ORIGIN      
        1 atggagttta taaaggtgat tttcattttc aaaatgctga gactattgaa cgagaacatc
       61 attatttcga tttattagtg aatacagtgg tagaattagc gaaatgggca aagtcaaacg
      121 aaacttatag tcaagtactt aaaagaatta tgagtcaaga aacttcattt atctatgagt
      181 taagcaagtc aaatttgaaa tatacttata atgaagatga acttgaaatt ttagataatc
      241 cagaaaattc acaatggatt aaagagttga agagagaaga ctttattgag acactaagaa
      301 ttctaaaatc gttatttgat ttgaaattag ccaaagctac catcatggga attgaaagag
      361 attcatatta ttgggaggaa attggactat atgctcccca gattgaaggt gaagataaag
      421 agaaaaggat agacaacatt attaactata ttaatagttt aagtgaacct ttaaaatgta
      481 gaggacttga ttttatagga acattaggat ggacagaaac atactatgag aaaatatata
      541 aagaaataat tgaacaatat tttcctaaat aatttaataa ggattgattt atcgtaaaag
      601 aaaaaagttg tgatttccaa aatcacaact tttttctttt gtcaagattt tgagtgggat
      661 atatttttgt agaactgaag atcatgaaat tatgagaggt tggaggtaat gtagtcatac
      721 aaataaaaat tttttagaaa gtgagtggta attaatgaaa atgaatcaca cttcaattca
      781 gaagcattta caactgttaa atacactggg gatttcttat gttaatttga gaactttttc
      841 tacattaaac aagaaaaccg tttctcatat tttttctact gcagattctc aagcaattat
      901 taactattta gaaaaacaga aattgtctga aggtacagta aacataactt ataatcctat
      961 taaacaagag gtgattacac aaaaaaagca ttctgtgaga gacaatgata ttgaaaaaat
     1021 tcggtttgta tttattgata ttgaccctaa aaggaaagag ggaagtgcaa cagatagcga
     1081 aaagaaaaag gcagaaaatg ttatggaaca agtagaacgt tatttgaaag aaaagagaat
     1141 agaatcattt gtaaaagttg atagtgggaa tggctaccat atttttcttc ccataaatga
     1201 acaaccaaat aatcacgaga cgattcttac tattcagaat tttcttcaat tacttcaccg
     1261 aagatttgga gtagaagatg aagttgatat tgatacttct gtttataatc cttcaagatt
     1321 atgcaaatta gttggtacac cagcggtcaa aggaaagtcc acaaaagagc gtccgcatcg
     1381 tttatctagt ttcctttcta ttcccgataa catagataga agtaattgtt ttaaaattct
     1441 tgaacagata gttgaagaaa attccatgat gtcaacctct agagtaaaaa gccatccatc
     1501 ttttagagaa gtgttacctt ttattcaagc agactataaa caatggttag ctcactatcc
     1561 tgaattaact tataccataa aagagggcga tcataaagga gttaccttac ttatatttga
     1621 cgaatgtcca ttaagaaaac acacgaataa ttctaatggt tcttgtttgt ctgttacggc
     1681 tgataataag attcgatttc attgtctgca tgcttcggat aaaaataaag atattcaaga
     1741 ctttgtaaaa aaatatccgt tgccaaaacc tgtaaatcct tctgtagaat cattagaatc
     1801 caatcaagaa tattattttg accagtttaa actgaatcaa gaaggagtat ttttggtgaa
     1861 agaaggtcaa gaagaaaaag ttgcttgtcc gatgtttatt aagcaaatac gcaaaaatat
     1921 cgaacaaaaa gtcattacac ttgttcttgc ctattatgtc gatcattgtt gggatgaaat
     1981 agaaatagaa ggtggatgtt tacaaactaa taattttaaa caattagcaa aacatggctt
     2041 aatcattaaa gctagaagag aagctgaagt cgttgacttt ttacagattc aacgggagaa
     2101 tgctccaatc ttttttgaac atgaatctct tggctggaaa aaagaaaatg aagcaatgat
     2161 ttttcagtta caagattctt ttggacgtga aattccttct gtattaacta aaaactcatg
     2221 ctaccaatta acaactaatg gtacagaagc ggaatttgac gcattggttc ataagtatgt
     2281 tttaggaacg aacttagaac ttgcttggtc gattggtatt gcttctattg tgttaggcta
     2341 tttaaacgtt acgaaggcta atactttttt gagtttacta attaacctac agggcaagag
     2401 cacaacgggt aaaaccacta cgttacactt tattgcctct ttatttggaa atccaactac
     2461 ggttcttaga aatatgaatg ctaccaataa agccattata aagctagcat caaacaatca
     2521 gggaggtgtt ccactaatac tagatgagtt aggagcagca actcaaaata actttagctc
     2581 gttgatctat caattagctt caggagaaga aaggctaaga ttagattcta attgtcagct
     2641 tatgccacaa gagacttttt ctgttttgac ccttacctct tctgaagagc gtttggaaaa
     2701 ttatttcaat gagaaattaa ctggtttgaa agttcgatat atcgaatttt ctgatatcaa
     2761 gtttacaaag gatgcgaatc acgcagagaa agttaaaagt ctttcttcta atacttttgg
     2821 ttggggtata aagttatttc ttttgaaact ctttgaacaa gggattacaa aaattgaagc
     2881 aacttttgtc gaagctcaaa aaaaactatg ggctgtttta cctgaacatt ctttgaagca
     2941 acgcattggt aataatctag ctttgattta tagtggagcc ctcctaattc aagatatact
     3001 gggctatcct ttacgattag agcatataca gacggtgctt attaattcgt acgaacagtt
     3061 aattgtagat actagtttag gaagtgagca aacggtattt gaccgagtga aaacattgtt
     3121 ggtgatgaat agccataaat ttttaacaaa cagtgacaac aaacatacgc ccagtacgat
     3181 ttggggaaaa gtatcaattc aaaaaaatgg ggatattaag gtaaatattt ttcctatccc
     3241 gtttgaggac ttgttaaggc gagaatttca agtaaaggat ctaacctatc tttttaggca
     3301 attgcttgat tcgggtaata tgtgtgcaga aaaagggaga agaacgaaac gtattcacat
     3361 gaatcgtcaa gttcttccaa catacgagat tctattacct tcttcactca aatcgtattt
     3421 tcgattgtaa tttagaataa accaaagtat gaaagtgagg caccatgtta tggaaatatt
     3481 tcttattgat ttagcaaaac aactgattac ttatctgtat gatagcgagc aaacccaaac
     3541 ggaagtaaat gaagcttgat ctatgcttca tttacttcat tcaatcaaac atgaaagaaa
     3601 ggaaaattct aatgactaaa aataaaatga cactaaaagc agaggttctt ctgtatattc
     3661 aagaacattt ttcaaatcaa gcctttttta caaaaccaat ttacttggat ttcgaaataa
     3721 gaggggtatc tgctggatct atcgggggta ctcttcaagc gttgaagaac gaaggatatt
     3781 tagaaaatca ctttgtgcaa cgttcgttca atagaagagt cgtgaaaaaa tggtatttag
     3841 ttcattcata gagaggggaa tcaagcatgc gtgtggcttg ttatgatcga acgtctgttt
     3901 atgaagaagc gaaaattagt aatgacgctc aatatgatgc agtgaaagag tatgttttag
     3961 agaaatatga acaaattccc caacgatttt cagacaaagg agaatcaggg gaaaaagcgg
     4021 agcgtccagc ttttcgacag cttagagctt gggtttgtgc gtcagaagaa gagggggtga
     4081 tctatgtttg gcgttatgat cgattggcac gtcaagccaa aatagcctta gaatttattg
     4141 agttgtgcca acaacatcag gttcaagtgt attcgatttc tgaacctctt cctgcttctt
     4201 tctcacacgc gtcaacgaat aaactatttg tgcaattatt gttcactttt gcagaacaac
     4261 aacgaaacgt cattattgag aatgttcgtg ccggactttc ctataaaaag caacaacggc
     4321 aatatttgtc ttctgcggta ccatatgggt atcgtttagt gaatgggaaa atccaacaag
     4381 aagtacacga agcaaggatt gttcgctata tttttcaact ttatttgacc aaaaaatatg
     4441 gctacaaaaa gctttgtcaa cgattgacgc aacaaaagct tttcttccgc gaacgtcctt
     4501 ttcagcccta tcatatttat agtatcctaa aaaatccatt gtactatggc gaaatcaaag
     4561 gaggatcttt ggggaagtat ctaggaacct ttgaaccgat tctctcgaaa gcaacctttt
     4621 tacaagccca agagattcgc caatcaaggt gtacagctaa aaaagatacg tatctgtact
     4681 tattacgtca aaaaattcgg tgtcccttct gtggtcgtca tctgagttcg aagtatcaat
     4741 ggaatacaaa gaagacgaag acgcttcatt attatcattg taccgaccga agctgccaag
     4801 gaatttttct tcgggcagaa agggttgagt cagaagtatt ggaaaagata caggcttttt
     4861 ttcaatacga aggcgtctat caagccttga ttgcccaatt aacgcaacaa ttagccgaaa
     4921 agcgaaaaga tgaaaaacgc aaagtaatcc aacaaaagaa acaagaagca caatggatta
     4981 aggcatttga agagggcaat tgttcactgg aagaactcaa gcaacaactg caagctcttc
     5041 aaaagccgat gtctacttct tcgtttcttt cactccaaca gtgtgaagca aaagtgaaag
     5101 aatggcttca actaaaagac ctctctagtc gacaattatt atgtgactat gtttgtcgga
     5161 ttgaggtaac aaaggataaa acagtttgtg ggatttactt ccgacaactg acacaaaata
     5221 ttttagaaag gaacgaatga tctatggaaa tgatacaaac caaacgagtc gcattgtata
     5281 ttcgcgtgag tacacaagaa caagctttag aaggacatag tattcaagca caagaacaaa
     5341 atggacgaca gtttgccgaa cggatgggct atgaagtggt agaagtttat gccgatgaag
     5401 gaatcagtgg gaaatccaca aaaaatcggt tcgcctatca acgaatgatg aacgatgctc
     5461 ggaaccatcg ttttgatcta ctagtgattt ggaaattgac ccgtctcgga cgcaatatgt
     5521 tagatgtctt acaaacagtc gaggaattat tgcgatatga cgtaggatta cattcgatct
     5581 cggaacaatt tgacgtgacc acttcgacag gtaaattgat gttacaactc cttgggagtt
     5641 tcggtgaatt tgaacgcaac caaattgcgg agaatgttca aatgtcaatg caaagcttag
     5701 tgaaagataa aaagcgttat gcaggaggcc gacgtttagg ctatgtcagt ggtattgaca
     5761 cgaatggtca aaaacaattg gtcattgaac cagaagaggc gaaaattgtt cgtctgatct
     5821 atgcaaaata tttaggcgga gcagggtatc gagcgattgc caattttttg aatcgtcaag
     5881 gatatcaaac agtcaaaaag aatgcttttt ctaccacggc agtcaaagat atcttacgca
     5941 ataaaattta tgcagggtat ttagaatatg cgcgctacat caattgggaa accaaacgac
     6001 ggaaggggaa gaatccgaga ccgattttag tgaaaggaac ccatgaaccg attatttcgg
     6061 aagaagatta tcaaaaagta caagaacgcc ttgctttaga aggaaaacaa cccaaatgga
     6121 accatacagg tgaaaatgta ttgacaggct tgttacgttg tccagaatgt ggcgcaccga
     6181 tggcagcgag taatgtcaca aataccctaa aggatggcac aaagaaacgg attcgatatt
     6241 attcttgttc agtctttcgg aataaaggag caagtgtgtg tcatgccaat tcgattcgtg
     6301 ccgaacaagc ggaaacattt gtcgccgacc gattaaagga gatcgttcag ctaccagaag
     6361 ttcttccccg attggtggca gcgttaaatg aagaaatagt gcgacaaagc caaccattgg
     6421 aacaagaact cgtggtgtta ctggaacgaa aggaagaatt gaagactaaa attgaaaaat
     6481 gggaagctgc tttggaagat agtcctgaat tgtttccgat gttaaaagac cgcttagacg
     6541 aattgactga aaaacgacga cagttgcaca ttcgagaaaa tgaaatctta gggatctttc
     6601 aacaaggaga gccgattcaa gtcaaagatg tccaacggat attgactaat ttggatcgtt
     6661 ttcttgccca gagtgagaag aagcaaatca aagccttgta tcgtaccttt atagagaaaa
     6721 taacctttga tcctaaccat aaggagatgt tggaaattac gatgaaattc acgcatgccg
     6781 tggtacaaca attgaatgag caatatcaaa tagccgtatc caaaacaaag gatacggcta
     6841 tttttgtgtt aaaaacaccc tttacgttaa cgatctagtc taggttatat caaacaacgt
     6901 tagaggggcg ctaacgcttc tctagcgatt tgaaatcgtt agagaagtag ttagggaggt
     6961 ccttatcata aatagggata tttattaatg aatatttgaa atataattgt atataagaat
     7021 taaaaagagt ggggatagat atgaaaaaga tgggtaagtg tattttatgt gaaaaataca
     7081 ctgaacttac taaagaacat gtaccaccta aaaaatcagg gaatactgga ggtaaaaaga
     7141 ccagaactgg taatttagat gatttcctta agagcgattt tacaaaaggt gattttccga
     7201 aggggattaa aaggaaacct caaggaaatg tttattata
//
LOCUS       DARBER010000124         7013 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DARBER010000124 DARBER010000000
VERSION     DARBER010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.128"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(265..447)
                     /locus_tag="SCK06_002356"
                     /pseudo
     CDS             complement(265..447)
                     /locus_tag="SCK06_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            434..631
                     /locus_tag="SCK06_002357"
     CDS             434..631
                     /locus_tag="SCK06_002357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152315.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(674..1249)
                     /locus_tag="SCK06_002358"
     CDS             complement(674..1249)
                     /locus_tag="SCK06_002358"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1152316.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1404..1930)
                     /locus_tag="SCK06_002359"
                     /pseudo
     CDS             complement(1404..1930)
                     /locus_tag="SCK06_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2387..3898
                     /gene="gadC"
                     /locus_tag="SCK06_002360"
     CDS             2387..3898
                     /gene="gadC"
                     /locus_tag="SCK06_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEG1152317.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3911..5311
                     /locus_tag="SCK06_002361"
     CDS             3911..5311
                     /locus_tag="SCK06_002361"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEG1152318.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5476..6916)
                     /locus_tag="SCK06_002362"
                     /pseudo
     CDS             complement(5476..6916)
                     /locus_tag="SCK06_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2188 a         1195 c         1251 g         2379 t
ORIGIN      
        1 gtaatatttg ccctggattt atggatcaga ttattgaaaa tgagtcaatt gaatcaattg
       61 aaaactatgt tcctgttgac ttaccaaaat agaaaaaata atcctgtttt tacagtgaat
      121 tattttaatt ttgattttca ataaaaatat attacaataa taatcgtaat agctaatgaa
      181 acaatttctc aatcttttta ccactccaaa cagggagcgt caataatttt gtgtaaataa
      241 ctcgtccttc tgcaaaataa tgggttactc agtaaacatt gaagctaatg tatccgtcac
      301 ttgttggaag cctttatggc ttctatttaa gaatttttga ttgtatgtat caaagatgga
      361 tactaggaaa cgctctagtg attcttcatt ttgaaactgc tcttttctgc ggctgtattt
      421 cttgatttgt ttattgaaag attcgattgt agaaccagaa ggaatggaga acacttttga
      481 actttacaac aaaactactg ttgataaatt aattccaatt ttagttccaa ctgatttaaa
      541 tggtcatgat gttttacttc gttttaacgg atttttaggt gaggattata gatacctaaa
      601 aatttctgat cctcaacctt taacagaata ataaaaaacg tgtaatcaat tatttgatta
      661 cacgtttttt atttcaatct tcgttgtagt agtggttgcg acataccgaa aggtaatctt
      721 cattacctcc tactacaatc tgttcacctg aataaactgg aataccatca acaatacgag
      781 cattcattac agccttttta ttgcaaaaat ggcaaatagt tttaatttcc tcaattttat
      841 cagcaaatgt caataatgct tcggatccct tgaagagatt attttgaaaa tcattcttca
      901 atccatagca aattactggt acttcctcat tcattacgat aagtgataga tcaataatat
      961 tctctctact taaaaattga gactcatcga ccaaaataca atcaactttt ccgtactgat
     1021 caataatctc cactaattct ccgtacacat ccatttcaga agtaattgca atagctggtt
     1081 tcgaaacacc tatacgactc tcaacttttc caaatcctga tcgagtatct attgcgcttg
     1141 tcattatgat aactctcttc ccttgttctt cataattatg agccactttc agtagctcaa
     1201 tactctttcc actattcatt gttccatatc tgtaaaataa ttgtgccatt aaatttattc
     1261 ccttctttta caattcaatt attccttaat tccatctgct tttatgcagc tgctcgttta
     1321 ttccttgata tgtctataac atctactatc cctaaaacca atttataagc cattagagat
     1381 ggtattcctg aacatctctc atattgtttt ttgagtggta tatcatctga aaaactgaag
     1441 gaatcatgaa aatagcgaca tacttatcat ttagagttct aactttaaat gcatttactt
     1501 cttcagtcag cactttagtc atgttggaca tggtttgggg cgtgtagtga tgcccataca
     1561 ttttctcaat caagtctgcg atttccgaca tggtaatacc ttttcggaag aggtgaatga
     1621 cggtctcctc taatgtgtca ttcgtccgtc tataagcagg aacagtctgt tgcttgaact
     1681 cgccattgcg gtcgcgcgga atctggagat gaagttcccc atactcggtc ttgaccgtac
     1741 ggtcatagga gccgttacga gaattacccg tgttaaaacc aatgcgatcg tacttttcgt
     1801 aatcgaggaa agccgttaac tccgttttga gaagcgtatt gacagctttt tccaaatgga
     1861 cacggaataa tccattcaaa tcgcctttgt tcgctagagt ctttaggatt tctgtagtaa
     1921 aatcgttcat atggaagtcc tcttttctgt gaatgtgttg tggtaactct attctacaga
     1981 agggacttcc ttttttctat ttacacaaaa tattttacac tctcaacaaa agtatcttcg
     2041 attaatactt gctcaagtta tgtgcagcta cgaaaaagta ggtaccgagg tactttcttt
     2101 ttcgcagctt tttttggact tatgagaagt acattcgctg gaagaatacg atataaaacg
     2161 agctttttca ctaaagatat taatagagtg aaagaaacaa gcttttgttg tcagtttttt
     2221 cacttttcta aaaatgaaaa taaaaaattc tggaagtgga gaagaatttt gcttgctata
     2281 ctttgtcttg tagaaaagag gtggacaaaa taatgcaagc tcaaataagc gattttccat
     2341 aaccttggta ggaatgacaa attaaatagg aaagggaagt aataatatga ataaaaaaaa
     2401 gttatctcta ttcggttttt ttgccctgac agcttccatg gttttgactg tttatgagta
     2461 tccgaccttt gcaacatcac aattacattt ggttttcttt cttcttttgg gaggtatttt
     2521 atggtttcta ccagttgctt tatgtgctgc agaaatggca acagttgaag gatggaaaga
     2581 tggtgggatt ttcagttggg ttagtcaaac tttaggagaa cgttttggtt ttgcagctat
     2641 ttttttccaa tggtttcaaa ttacagtagg ttttattaca atgatctact tcatactagg
     2701 agcgctgtcg tatgtaatta attttccaga gcttaataca gatccattta tcaagtttat
     2761 tggtgtatta gtcattttct gggtactgac tttttcacag ttgggcggga ccaatcgtac
     2821 cgctaaaatt gctaaagcag gatttgtcat tggaattgtt attccttcga tcattctctt
     2881 tggtttagca gcagcctact ttatcggcgg taatccgatt caaatggcta ttaatgcgaa
     2941 agcttttatt cctgactttt ctcagctttc aactttagta gtatttgtat cttttatatt
     3001 ggcttatatg ggcgtagaag cttcagcatc acatattaat gagctggaaa atccccaaag
     3061 aaactaccca ttggcaatgt ttttactagt gattttggcc atcgccttag atgcaatcgg
     3121 cggtttctca gtagcagcgg ttattccaca acatgacttg tctctcagtg ctggtgtaat
     3181 ccaaacattt gaaacgctta ttttacattt taacagtcat ttacattggt tggtaaaagt
     3241 gattgctctc atgattgcct gtggcgttat gggtgaagtt agttcttggg tagttggtcc
     3301 atcacgaggg atgtttgcag ctgctcagcg aggtctgtta ccagttttct tccggaaaac
     3361 taataaacaa gacgttccag ttccgttgat tatgatacaa ggaattatcg ttactatttg
     3421 ggcagcagtc ttaacttttg gtggaggagg caacaattta tcattcttag tagcaatctc
     3481 cttaactgtt gtcatttatc tcgtaggata tttactattt ttcctaggtt actttgtatt
     3541 agtttttaag aaaaaagact taaaacgaac ttacaatgta ccaggaaaaa ctgttggcaa
     3601 aacgattatc ggtggaattg gttttatttt atcagtgttc gcattgctta tctcttttgt
     3661 accaccagct tcaattgcag ctagtgagca ccgaacatac caaatcatac tacttattag
     3721 ctttgtagtg actgttcttt taccttttat catttatgaa gtacatgata aaagtgctca
     3781 cacaactatt gaggaaccta ctcacgtgaa agctgaagac gttaatcccg ctgtttatcc
     3841 agctgctaga ggagagcacc acgttacaaa aaatgatgaa catattttaa aacattagga
     3901 ggaattcatt atgttatacg gaaaagataa tcaagaagaa aaaaactatt tggaaccaat
     3961 ttttggctct gcaagtgagg atgttgactt gcctaaatat aagttaaaca aagaatccat
     4021 tgaaccacga attgcttatc aattagtaca agacgagatg ttggatgaag gaaatgcgcg
     4081 attaaactta gctacttttt gtcaaacgta tatggaacct gaagcagtga aattgatgac
     4141 ccaaacgtta gaaaaaaatg caattgataa atcagaatac ccacgaacaa cggaaattga
     4201 aaaccgctgt gtaaatatga ttgctgattt atggcatgct ccaaataatg aaaaattcat
     4261 gggaacttca acgatcggct cttcagaagc ctgcatgctg ggtggtatgg ccatgaaatt
     4321 tgcttggcgt aaacgtgctg aaaaattagg tcttgatatt caagcaaaaa aacctaacct
     4381 ggtgatctct tctggttacc aagtttgttg ggaaaaattc tgtgtatatt gggatgtgga
     4441 actgagagaa gtcccaatgg atgaaaaaca tatgtcaatt aatctagata ctgtcatgga
     4501 ttatgtggat gagtacacaa ttggtattgt aggtattatg ggtattactt acactggtcg
     4561 ttatgatgat atcaagggtc tgaatgattt agttgaagct cacaataaac aaactgacta
     4621 taaagtatac attcatgttg acgctgcatc gggtggcttt tatgcaccat ttactgaacc
     4681 tgatctagtt tgggattttc aattgaaaaa tgttatctca attaattctt caggtcacaa
     4741 atatggtttg gtatatccag gtgtgggttg ggtcttatgg cgtgaccaac aatacttacc
     4801 agaagaatta gtatttaaag ttagttactt aggtggagaa atgccaacta tggctatcaa
     4861 cttctctcat agtgcagcac aactaattgg gcaatactac aactttgtac gctatggctt
     4921 tgatggttat cgtgatattc accaaagaac tcatgatgtt gctgtttatt tagccaaaga
     4981 gatcgaaaaa actggtattt ttgaaatcat taatgatgga tcagaattgc cagttgtgtg
     5041 ctataagctg aaagaagatc ccaatcgcga atggacacta tatgatttat ctgatcgtct
     5101 gttaatgaag ggatggcaag tcccagccta cccattacct aaagacttgg atcaattaat
     5161 tattcaacgc ttagttgttc gagcagactt tggaatgaac atggctggtg attatgtaca
     5221 agatatgaac caagcaattg aagagttgaa taaagctcat attgtttatc ataaaaaaca
     5281 ggatgtgaaa acgtacggct ttactcacta aaattcttca gagaaatata aaagaagtta
     5341 attaactaat aaaaggagtt gtgattacca cacagctcct taaatctatt tgaataaata
     5401 tatgatattc atttgttaca ctctcaatct tagctgttgt tctattcatc attactctta
     5461 ttgtaagtag ttcagtcaaa tttgcttgtt caattgatcg ataacagtat gaattgtttg
     5521 aaaagtaatc ggatatataa ttgttttctc actacctaag ccttcttggt ataaagaact
     5581 atcgcaaagt ataatccgct gagtacaaaa gaaagtgtcg tctactattt ctctaagttg
     5641 tttacaaatg atattactaa tagaaaagtg ttctcccaaa atgtcttcta actgttgtcg
     5701 atagtataaa tacctaaagt ctgacggagc tactatgaac acttcaattg gagatttccg
     5761 tttatgctca tttaaaagac ttaaactaat ggttagttta cgaatagaat tctcattcca
     5821 acgcagaaat aaattgtttt gttttcgcca ttgttcaaga atttcatgaa cttctaagta
     5881 caaagcgcgt tgctcttcat ttaacaagta agtcttatca ggttgaaata tctgaatatc
     5941 agcccaaagt gagcggataa acgggagaag tgctttatca aaaagtatct gattttcttc
     6001 agatatattc agagataaga taagtttttg actgagctca atcaccaaag ggttatctgc
     6061 tacatggttt tgataaacaa cgttcaagtc tttttgtaac aattcaaagt tatcgcatag
     6121 ataatttcta gagttgaata gaaagtagat ataaaggcat tcatcttcgt catgacataa
     6181 agagaaagaa agttgctcta atcctttctg tattagctga aacaagggag tatgtcgggc
     6241 aactcttttt tcttcctgac taaaatatat tgggttagtc ttagatctct gcaaaccgat
     6301 agcgatacct aataaaataa attgtttttc aactgtaggg taacttcttg aaaaaaaatg
     6361 tttttctaca taatcaatca attgttgacc gctttgaata attgatttat ttagctggat
     6421 caactctgtc cgatttgtct catagattat cgttagtaac aaacttcgat aatccttttc
     6481 aggaatttca aaatggccat cagcatttaa gtactgcatt tgtttaaaaa aattaatcaa
     6541 ctcatttcgt aagttgtaaa ctttgcttaa cgaaatgaag gtagtatcag ctatattact
     6601 ccaattgtgt tcccctgata aataatgtcc tagtacattg aaaaagtcag agcgctgata
     6661 gatgagttga gtgagtgcca acctcgaaaa cgaagagtcg aatgcgactg taaagctact
     6721 tctagatttc tccactgaaa taatttgctg atggagtatg tcggtaatgc attccaagtc
     6781 gttagtgaca gacagtgagt gggcaaacgt tcaattgatc tgctatttcg cgaatctgaa
     6841 tggtcccttt ttcgtgtaat tgctcacaaa tgtaaacttg acgaaaaata cttttctcca
     6901 gataggtttc caacatagta ctcctcctag tcattttata cataactatt ataactctac
     6961 caatcggaat agtcctgtaa tatattgaat caaaaatata aattataaaa taa
//
LOCUS       DARBER010000125         6886 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DARBER010000125 DARBER010000000
VERSION     DARBER010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.129"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..182)
                     /locus_tag="SCK06_002363"
     CDS             complement(<1..182)
                     /locus_tag="SCK06_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1152319.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="SCK06_002364"
     CDS             517..951
                     /locus_tag="SCK06_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1152320.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="SCK06_002365"
     CDS             974..1294
                     /locus_tag="SCK06_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152321.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="SCK06_002366"
     CDS             1435..1626
                     /locus_tag="SCK06_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152322.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="SCK06_002367"
     CDS             complement(1697..1954)
                     /locus_tag="SCK06_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG1152323.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="SCK06_002368"
     CDS             2112..2375
                     /locus_tag="SCK06_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152324.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="SCK06_002369"
     CDS             2399..2587
                     /locus_tag="SCK06_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152325.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="SCK06_002370"
     CDS             complement(2670..2855)
                     /locus_tag="SCK06_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152326.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="SCK06_002371"
     CDS             3070..3441
                     /locus_tag="SCK06_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1152327.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="SCK06_002372"
     tRNA            complement(3591..3663)
                     /locus_tag="SCK06_002372"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="SCK06_002373"
     CDS             complement(3709..4137)
                     /locus_tag="SCK06_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152328.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="SCK06_002374"
     CDS             complement(4197..4955)
                     /locus_tag="SCK06_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG1152329.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="SCK06_002375"
     CDS             5250..5447
                     /locus_tag="SCK06_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152330.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="SCK06_002376"
     CDS             5553..6035
                     /locus_tag="SCK06_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152331.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="SCK06_002377"
     CDS             complement(6093..6689)
                     /locus_tag="SCK06_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1152332.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1059 c         1353 g         2143 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga attttt
//
LOCUS       DARBER010000126         6868 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARBER010000126 DARBER010000000
VERSION     DARBER010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.130"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            154..1026
                     /locus_tag="SCK06_002378"
     CDS             154..1026
                     /locus_tag="SCK06_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1152333.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1023..2981
                     /locus_tag="SCK06_002379"
     CDS             1023..2981
                     /locus_tag="SCK06_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1152334.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3313..4164)
                     /locus_tag="SCK06_002380"
     CDS             complement(3313..4164)
                     /locus_tag="SCK06_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1152335.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4287..4754
                     /locus_tag="SCK06_002381"
     CDS             4287..4754
                     /locus_tag="SCK06_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG1152336.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5017..5499)
                     /locus_tag="SCK06_002382"
     CDS             complement(5017..5499)
                     /locus_tag="SCK06_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG1152337.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5599..6462)
                     /locus_tag="SCK06_002383"
     CDS             complement(5599..6462)
                     /locus_tag="SCK06_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1152338.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         2051 a         1417 c         1250 g         2150 t
ORIGIN      
        1 caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg
       61 tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa
      121 cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa
      181 aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga
      241 tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg
      301 gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta
      361 tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa
      421 catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt
      481 atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt
      541 attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac
      601 gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat
      661 cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg
      721 gaaacagata tccattcaga accattttca ctgaattatt caagactggt gaatcattta
      781 cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta
      841 gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg
      901 ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat
      961 atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa
     1021 aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac
     1081 ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg
     1141 tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg
     1201 ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt
     1261 tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga
     1321 atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta
     1381 ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga
     1441 tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt
     1501 tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt
     1561 ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc
     1621 gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag
     1681 caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa
     1741 tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta
     1801 tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag
     1861 cttttgcttc attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg
     1921 cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat
     1981 tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt
     2041 tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag
     2101 tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc
     2161 gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg
     2221 cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg
     2281 ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata
     2341 aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca
     2401 tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg
     2461 caacacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga
     2521 tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag
     2581 atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag
     2641 aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg
     2701 gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact
     2761 taaaaggcga accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat
     2821 tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg
     2881 ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa
     2941 aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac
     3001 ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg
     3061 aataaaaacg gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat
     3121 gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac
     3181 ctctgataaa cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat
     3241 gtggaaagcc attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa
     3301 cctctttata cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa
     3361 tggttttccc aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat
     3421 atcatgttgg atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag
     3481 attttcgaca aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg
     3541 ctctaatgcc ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc
     3601 tggagagtca ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc
     3661 ccaacctgtt tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc
     3721 aatgataggt aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc
     3781 tgaatcctta atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg
     3841 atcggcatct gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt
     3901 cactacattc ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg
     3961 atctattcct ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat
     4021 attaaatcca gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat
     4081 ttcattttgt ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt
     4141 tgctcctgtt actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt
     4201 tacagctgta catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag
     4261 caaaaaaaat gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc
     4321 atgtacttgc ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg
     4381 ccagtagtat cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat
     4441 ccggtttgat ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc
     4501 agatttcttt actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg
     4561 atccaaaaac aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa
     4621 gctacgctcg gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc
     4681 tgatacataa aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag
     4741 agagattttt ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata
     4801 aattctttta ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct
     4861 caggtcaaag cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg
     4921 gcattagttc tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc
     4981 tcaggtcaaa gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat
     5041 ccagatttct cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc
     5101 cccaacatag gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa
     5161 atcatttctt aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt
     5221 tgggaatggg atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata
     5281 gtacatcatt ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc
     5341 ttgtttcaat gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc
     5401 tgcagccaaa ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac
     5461 cgtaaaatct ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat
     5521 attaagttca tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg
     5581 gatattttta ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac
     5641 attactgaaa tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata
     5701 atctgaagaa ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg
     5761 aaatggccct ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa
     5821 atcagcgtac ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt
     5881 ttcttccaag cgggctccat caaatccata agaaacgttg attacttgat ttggatataa
     5941 atctcctaac ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg
     6001 tatttcttga ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata
     6061 aaactgttct ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa
     6121 aaatgtcaag cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata
     6181 tcctgaccaa tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa
     6241 cgccgtttct gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg
     6301 gtttttgata tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg
     6361 atattcagag attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg
     6421 ttcttcgatg taatcgatca attggttcag atctttaagc atatttatct tcctccttgc
     6481 ttgccttttt atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta
     6541 gtcagatgta gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg
     6601 gataattggt attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag
     6661 acacattcta agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta
     6721 ctacctactg tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt
     6781 ttctttgcga tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa
     6841 tcacttcatt tttgtcagat aaataaaa
//
LOCUS       DARBER010000127         6771 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARBER010000127 DARBER010000000
VERSION     DARBER010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.131"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..151)
                     /locus_tag="SCK06_002384"
     CDS             complement(<1..151)
                     /locus_tag="SCK06_002384"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="catalyzes the formation of phenylpyruvate from
                     prephenate in phenylalanine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG1152339.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVP"
     gene            complement(148..657)
                     /locus_tag="SCK06_002385"
     CDS             complement(148..657)
                     /locus_tag="SCK06_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG1152340.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(650..1945)
                     /gene="aroA"
                     /locus_tag="SCK06_002386"
     CDS             complement(650..1945)
                     /gene="aroA"
                     /locus_tag="SCK06_002386"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG1152341.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(1958..3049)
                     /locus_tag="SCK06_002387"
     CDS             complement(1958..3049)
                     /locus_tag="SCK06_002387"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG1152342.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(3066..4232)
                     /gene="aroC"
                     /locus_tag="SCK06_002388"
     CDS             complement(3066..4232)
                     /gene="aroC"
                     /locus_tag="SCK06_002388"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG1152343.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LREEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(4234..5298)
                     /gene="aroB"
                     /locus_tag="SCK06_002389"
     CDS             complement(4234..5298)
                     /gene="aroB"
                     /locus_tag="SCK06_002389"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG1152344.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(5331..6353)
                     /gene="aroF"
                     /locus_tag="SCK06_002390"
     CDS             complement(5331..6353)
                     /gene="aroF"
                     /locus_tag="SCK06_002390"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG1152345.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         1716 a         1588 c         1266 g         2201 t
ORIGIN      
        1 caggaacgac agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa acagggatgg
       61 aagcatacgc agtcaattgt tctgtaggaa atagctgaca agccgcctga taagtgaatg
      121 aactttctgg ccctagatag ctgacgttca tatgcttttg atcctttcta taatggtatc
      181 gatgatcttt tctggtgttt gattggtcgt gttgattcga tgtgtcgcac tttcttcata
      241 ccaagaaagt cgttgtgcca ataatgcttt tatttcttct cttgtttttc caagagctaa
      301 aggtcgaata gacgtttcat cttgttggat acgatcaact agacagtccg cttctgcctc
      361 taagtaaaga acaatcggct gtgtttttaa aaaacgccga ttttctgcac tttggacaat
      421 ccctccacca gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa tatctgactc
      481 cacttttcga aagctttttt caccgtaata gtgaaaatat tcggcaatcg acaattggat
      541 atgttcctca atctttgtat ctaaatctac atactctttt tgtaaacatt gagctaaccc
      601 tttaccgatt gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc tagccatgag
      661 cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa gagattgaaa
      721 cagcttcagc tccttctagt tcgcaaggtc catcagctat caaagcagct acttgcagca
      781 tcataccgat tcgatgatcg cctctgctgg acacttttgc gccatgcaga ggtgtcggcc
      841 catggatgat cagaccgtct gctgtcgctt ttatctttgc tcccattttc tgtaattctt
      901 ctgccaccgc atcaattctg ttggtttctt ttacttttaa ttcttctgcg tctttgatga
      961 tcgtgattcc ttctgcttga gtagccacta aagctagaat gggcaattca tcaatcagac
     1021 gcggaatgat ttctccacca atcgttgctt tcttcaaatg actcgtcttg acacttaaat
     1081 cagccgattg gttctgttca ttgtgattcg tctgggtaat cgctgcgccc atctcctcca
     1141 acacatctaa gataccagtt cttgttggat tgaccccgac attttttaac aacagttggc
     1201 tttccggaac aagaagtcct gctgctagaa agaaagcagc tgatgaaata tcacctggaa
     1261 cagttatctg ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt ttatcttcca
     1321 ctgtgatcct tccgccaaat tgacgaatca tttcttccgt atggttacgg gaacgttctt
     1381 tttcaacgat tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa gcagatttga
     1441 cttgcgcact ggctacaggc atattgtatc ggataggtga taaagagcgg gttcctgtca
     1501 cagtgattgg tagatactgg tcattttctt ctccttgcaa gtcagcgccc atctcgcgta
     1561 aaggttccat cactcgcccc attggacgtt tagacaacga agcatccccg aacagagtag
     1621 tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg tatggtcgta ccggagtttc
     1681 ccatgtcaat tgattcggtt ggttggacaa atgagtggct accgcgacca tgaatcttga
     1741 tcacctcttc ctcttcttcg atctcgacac ctaattgttg gaaggcatgt aaagtactca
     1801 gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca attgctccaa
     1861 acatgatgct gcgatgggaa attgatttat ctgctggtat cctaacagtc cctcttaatc
     1921 catgtatttg ttgtaataat tgcatttatc cagcctccta tttgatccag caatgataac
     1981 tggtattaga cataatgatt ctttttgctt gtaacaaatc ctgttggttt ttaaaactga
     2041 tttgcagtat tcctaaaata tcttccctgg tctcttggat cttcaaatta atcaaagaaa
     2101 ctcccgcttt tccgatcagt ccagttactt ctgcaataac gccaggttca tctggcacat
     2161 cgataaataa atcaaaaaag gcaggaatcg cgccttgctt atggacaggc aactggtctc
     2221 gactttcttt tgcccgattg aaaaaagaaa agatttccga ttggttctca gtcatgatcc
     2281 attctgctat ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat aatatctgct
     2341 tattgctcaa caaaatatct gtccacatga caggatctga ggaagcgatc ctagtgatat
     2401 ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata ctcttgattg aattgttctg
     2461 cctgattgac taagccagct gccagaatat gtggaaaatg acttagcatt cctgttatac
     2521 ggtcatgttc tcttgctgta agagttacat attttgcttt ggttccgcaa aatagttctt
     2581 gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa taggcatttt
     2641 caaatagttt agcatctgct gcctgtatac ctgatttatg agaaccagcc attggatgtc
     2701 caccgacaaa ccgaagattt ttctgctcag cggatgtcac gacttgctgc ttcgtactgc
     2761 tgacatccgt aatgatgacg tgatcagaca agttgattct ttcaaggaca tcaagataag
     2821 ctaaggttgt acggacggga acggctaata agataacgtc tgcttgttct gcacctgttt
     2881 caaaatcact tgcaacgtaa tcaacgatct gctgttctac tgcttgctca cgggtttttg
     2941 actgactatc ccaaccaact actatcagtt ctggattggt ctgacgaata caaagtgcca
     3001 tagaagcacc aatcagtcca agccccacga taaaaacatg ttgtgccatc ttatttgccc
     3061 cctttttagt aatgtttcaa gtagtcacgg tactccttca ctgcagcttt caactcaaca
     3121 agcgaatcac ttgggaattt tgacaatagc tctgtcgcta ccacagtggc tacaacattt
     3181 tccgcaacga cgctagctgc aggaactgct gtactatccg aacgttctac acttgctttg
     3241 taaggttctt ttgtatcgat atcaacactt tgaagtggct tgtacaatgt aggaatgggt
     3301 ttcatgaccc cgcgaatgac aagtggttcg ccattggtca ttccgccttc gaagccgcct
     3361 agattattgg tacgtcggaa atagcctctt tcttcttccc gtaggatctc atccatcact
     3421 tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa attctacgcc tttaaaggca
     3481 ttgatactaa ccacagcttg tgcgattttg gcatctagct tcgtgtccca ttgtacgtag
     3541 cttcctaatc cagctggaac gcctccaacg agcacttcta caattcctcc gatcgtatct
     3601 ccatttttct ttgtctgatc gatcaaatct tttattcttt gctcaactgc tggatcaaca
     3661 actcgtactt ctgacgcttc agaaatttct ttgatttctt gaatcgagat atttccaggt
     3721 atttctgctt ggattccacc aagacaaatc acgtgtcctg ctacgatgat gtcaagttct
     3781 tccagaattt tcttggcaat tgcgccgatt gctacacgca tggtcgtctc tcttgcagag
     3841 gaccgttcaa gcacattccg aagatctcga tgatggtatt tcataccgcc taccaaatcc
     3901 gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc aggcacttct
     3961 gctacagaca tgacagttgt ccaatttttc caatctttat tttctaccac taaagtgaca
     4021 ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga tccgaacacg atctttttcg
     4081 atcttcatcc ttcctcctct gccgtaacca gcttgtctgc gagcgagttc gtgattgatt
     4141 tcttcttctg acagaggtaa tcctgctggt agtccttcaa taatggctgt taattctggt
     4201 ccatgggatt ctcccgcagt catataacgc atcttacagt cctcctaagt aatctttgaa
     4261 tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc ttttctagca agatgatatt
     4321 caattggctt cctcttgctt tcttatcatg ggttatcgcg tggaataatt catctggatg
     4381 acttagatcg tacgtaatcg gcagatggaa tttttgaagt atcctttcta aagcagctgt
     4441 cgatccagtt ggagacaagc cgattttttc tgcatgagaa gtgatggcta ccattccaat
     4501 agctactcct tctccatgac tgactacccc atatcctgct gtattttcaa ttgcatggcc
     4561 aattgtatga ccaaaattta aaataagtcg atttccttgg tcaaagacat cttcttccac
     4621 tacttttcgt ttgatttcca acgtagcagt aatgattgga acagcatgtt ccagaagatc
     4681 tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta tagctgctga
     4741 tttgatgatt tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt ttagaacctc
     4801 aggatcaatc aatacaccag ctggctggct aaatgttccg acaaggtttt tcgctttgtt
     4861 tgtattgact gctgtcttcc cgccaatact agaatctact tgtgcaagta gcgtagtcgg
     4921 aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg ctagatcacc
     4981 caccattcct ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat tatctgctaa
     5041 aaaatcgtat aactcagctg cttgttctaa agatttactg gtctcacccg ccggtacgat
     5101 tgaaaggact ggttcaaaat tagcttgtct caattggaac atgatttttt ctgcatatag
     5161 tggagcaacg tttgtatcag tgatcacagc tactttttga ggtctccaaa tcgtttgaag
     5221 ccatgatcca gcctgttcta gtgcgccttt ttcaatatga atatcgtatt tttggttatc
     5281 aacatgtaca gtaagcattc gtttcacttc tttcttttaa aaagctggaa ttaatcgagt
     5341 gtctgaatct tttccattgc ttctttcatg atcgcaactt ctttcatcat gcgtgagtaa
     5401 gtcttttcat tcaacgattg gggaccgtct gaccaagcat ttgcaggatc aggatggatc
     5461 tctacgatca tgccatctgc tccgcttgcc acacccgcac gagccattgg tggtacaagt
     5521 tcccaaattc ctgtaccatg acttggatca acaatcacag ggaaatgagt taattttttc
     5581 atcaatggga cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta agtccgaatc
     5641 ccacgttcga taaaaatgac aggagatttg tcttcgactg caatgtattc tgcagcatta
     5701 agccattcat tgatcgtacc ggaaattcca cgtttcaatc caacaggttt acctgtttta
     5761 ccgacagcag atagcagttt aaaattttgc atattacgtg cgccaatctg taggatatcg
     5821 ctgtactcag ccaccatatc gatatgccct tcatccatca cttcagtaat cactttcatt
     5881 ccaaattcat cagccgcttg acggatgtat ttcaatcctt cttcttctaa accttggaaa
     5941 gcatatggag aagtacgtgg tttgaaggca ccacctcgca gaatttttgc gcccccggct
     6001 ttggccattc gcgcacattc tctgatttgg tctaatcctt cgatagaaca aggaccagcc
     6061 atcgtcacga aactgccgtc accaattttt actccgtcta catccacgac tgtattgcca
     6121 ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc attttctact
     6181 ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt tagtccaatc
     6241 actactcgtt ctgttccttg atcgattgag actttgaatc cttctttttc gatatgatcg
     6301 acgacttttt ttatttgttc ttccgttgct tcttttttca taattactat cattgttttt
     6361 cccgctttct tttgtttact tatctatctt ggctgatttt ctctactttt tcaaggtggt
     6421 tttacattaa ttcctactaa atgaccgtgt cctgttgttc tcattttcgc tccttctttc
     6481 ctacttatga cccagataat caacaaaaaa agatgtccct gtaggaaatt gcctctacag
     6541 tgaacatctt caacgagcct taaaaagcgc tgcaaaaata taaaatagcc actatagatg
     6601 aatttctcta cgtggctgta agtcgtgttt aaaaatcttt agccatcata gatacaaacc
     6661 tggtttgttt gtacccacga agtcatgaat acaaacctat ctaaaatagc taaagtaata
     6721 cgtattcagt tttgtaagtt ttctagattt cataaaaaat cgctccaaac t
//
LOCUS       DARBER010000128         6745 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DARBER010000128 DARBER010000000
VERSION     DARBER010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.132"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(24..857)
                     /locus_tag="SCK06_002391"
     CDS             complement(24..857)
                     /locus_tag="SCK06_002391"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG1152346.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(1049..1753)
                     /locus_tag="SCK06_002392"
     CDS             complement(1049..1753)
                     /locus_tag="SCK06_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1152347.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(1776..2402)
                     /locus_tag="SCK06_002393"
     CDS             complement(1776..2402)
                     /locus_tag="SCK06_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1152348.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            2728..5103
                     /locus_tag="SCK06_002394"
     CDS             2728..5103
                     /locus_tag="SCK06_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG1152349.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(5127..5618)
                     /locus_tag="SCK06_002395"
     CDS             complement(5127..5618)
                     /locus_tag="SCK06_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG1152350.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            5805..6725
                     /locus_tag="SCK06_002396"
     CDS             5805..6725
                     /locus_tag="SCK06_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1152351.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT         2166 a         1234 c         1312 g         2033 t
ORIGIN      
        1 taagagaagt gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata
       61 ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt
      121 aagaatttga tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca
      181 acagtttctt ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg
      241 cttgcgccaa acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc
      301 gaagcaccaa tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca
      361 agtgccaaga cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga
      421 gaagtccaac gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg
      481 taaacgatca aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca
      541 tccaaaggca aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt
      601 gtccacgtat cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga
      661 aaataaagaa cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac
      721 ataaattgtg aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta
      781 ctactgatgg gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata
      841 atatttgcaa aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg
      901 ttattttttg agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca
      961 gataagtaca cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga
     1021 ttctctggta aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc
     1081 cgttatcgat ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag
     1141 tgacctttgt tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca
     1201 gattaagcaa aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat
     1261 ttttttcgca gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt
     1321 cataagtttc cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa
     1381 aagaaacttc tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca
     1441 caccccacat ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc
     1501 ttttttcaat aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg
     1561 cagtttcgat tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta
     1621 acccgcgacc taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc
     1681 agccttgtac tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg
     1741 tagtttctgt cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc
     1801 cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca
     1861 ggtaatacaa acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac
     1921 agatcggttc taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca
     1981 aaaataaagc tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg
     2041 tttcctaaag tataagtttt catttcaaat tcatattctg caggcgagac aataacatca
     2101 gcaatatcat catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt
     2161 tgctctagtg ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca
     2221 ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt
     2281 ttgatttctt gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct
     2341 tcgttgtaga ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc
     2401 atgtgcattc ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg
     2461 ctaggaaaca aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga
     2521 aaagtatttg gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc
     2581 ttgttacatt agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag
     2641 cagggtgttt ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt
     2701 aatcttagag agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac
     2761 ttagtagaat ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact
     2821 gcattagaag gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa
     2881 aaagaagtct ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc
     2941 cgtgcagacg gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc
     3001 gaaccacgat ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg
     3061 gtgtatgcct atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact
     3121 tacttccaga cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag
     3181 ttagaaactt tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa
     3241 aactggcgca ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat
     3301 aggaaaggtc agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa
     3361 ttgtttgtag aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg
     3421 aaagctgtag gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact
     3481 agacaagtag ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt
     3541 gtcactttga cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat
     3601 cagtgcccat atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg
     3661 aatcatgaga atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt
     3721 tgtcctttcg aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac
     3781 aattatttat ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag
     3841 gactatctgt ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat
     3901 tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc
     3961 aaaaaacttc atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata
     4021 gcaaaagaag atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag
     4081 gcaggttatc aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca
     4141 tcacaagaac aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa
     4201 ttttatgatg atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa
     4261 gagttttgta ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt
     4321 attctttttt ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa
     4381 aaaagtctgg cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc
     4441 gcaaattacc tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag
     4501 acgattcatg cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat
     4561 caatatttag atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta
     4621 atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca
     4681 aatcccgctg aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc
     4741 gatttgagag gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg
     4801 aatcatgagc aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct
     4861 tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt
     4921 ggcatggcgg atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat
     4981 cgacgtttat tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg
     5041 gaagaaggaa tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa
     5101 tgacagacgt cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt
     5161 aagtcaagtt tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc
     5221 actctaatct gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct
     5281 gtagtgattt tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga
     5341 gctttgccac ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt
     5401 cttttgatca gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg
     5461 ctcaatacaa ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca
     5521 aagcgattgg acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg
     5581 acttctgata caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata
     5641 taaatattta ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct
     5701 aaaattcttc tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac
     5761 atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata
     5821 aaaatgaaag attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg
     5881 ctttaaatta ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc
     5941 aaatcatttc ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta
     6001 tttttctttt gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa
     6061 cgatccttag ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg
     6121 gacagatcac tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg
     6181 gcgtaggatt atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg
     6241 tactttcaca aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca
     6301 ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca
     6361 tttatgcaat gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa
     6421 tgatcgttac cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg
     6481 gaatgacatt actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga
     6541 tggtcatcac ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg
     6601 atgcatttat caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg
     6661 aacaaaaaat gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa
     6721 aatgaggaat caggtttatt ttaga
//
LOCUS       DARBER010000129         6643 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARBER010000129 DARBER010000000
VERSION     DARBER010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.133"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..859)
                     /locus_tag="SCK06_002397"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="SCK06_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="SCK06_002398"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="SCK06_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG1152352.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="SCK06_002399"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="SCK06_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="SCK06_002400"
     CDS             complement(2603..3421)
                     /locus_tag="SCK06_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1152353.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="SCK06_002401"
     CDS             3582..4271
                     /locus_tag="SCK06_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1152354.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="SCK06_002402"
     CDS             4285..5787
                     /locus_tag="SCK06_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1152355.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="SCK06_002403"
     CDS             5800..6276
                     /locus_tag="SCK06_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1152356.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="SCK06_002404"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="SCK06_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DARBER010000130         6570 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARBER010000130 DARBER010000000
VERSION     DARBER010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.134"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SCK06_002405"
     CDS             127..579
                     /locus_tag="SCK06_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152357.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="SCK06_002406"
     CDS             593..1282
                     /locus_tag="SCK06_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152358.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="SCK06_002407"
     CDS             1407..3758
                     /locus_tag="SCK06_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG1152359.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="SCK06_002408"
     CDS             3838..4233
                     /locus_tag="SCK06_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152360.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="SCK06_002409"
     CDS             4558..5175
                     /locus_tag="SCK06_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1152361.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="SCK06_002410"
     CDS             5216..5515
                     /locus_tag="SCK06_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG1152362.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="SCK06_002411"
     CDS             5549..5716
                     /locus_tag="SCK06_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1152363.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="SCK06_002412"
     CDS             complement(5761..6366)
                     /locus_tag="SCK06_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1152364.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DARBER010000131         6378 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DARBER010000131 DARBER010000000
VERSION     DARBER010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.135"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..613)
                     /locus_tag="SCK06_002413"
     CDS             complement(<1..613)
                     /locus_tag="SCK06_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152365.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINK"
     gene            complement(713..1222)
                     /locus_tag="SCK06_002414"
     CDS             complement(713..1222)
                     /locus_tag="SCK06_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG1152366.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1423..2292)
                     /locus_tag="SCK06_002415"
     CDS             complement(1423..2292)
                     /locus_tag="SCK06_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1152367.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2312..3433)
                     /locus_tag="SCK06_002416"
     CDS             complement(2312..3433)
                     /locus_tag="SCK06_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1152368.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3435..3830)
                     /locus_tag="SCK06_002417"
     CDS             complement(3435..3830)
                     /locus_tag="SCK06_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1152369.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3849..4655)
                     /locus_tag="SCK06_002418"
     CDS             complement(3849..4655)
                     /locus_tag="SCK06_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1152370.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4648..5436)
                     /locus_tag="SCK06_002419"
     CDS             complement(4648..5436)
                     /locus_tag="SCK06_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1152371.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5450..5926)
                     /locus_tag="SCK06_002420"
     CDS             complement(5450..5926)
                     /locus_tag="SCK06_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1152372.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5998..>6378)
                     /locus_tag="SCK06_002421"
     CDS             complement(5998..>6378)
                     /locus_tag="SCK06_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1152373.1"
                     /translation="DNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQ
                     NDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSV
                     VEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1973 a         1378 c          899 g         2128 t
ORIGIN      
        1 ttttattaat tagttcatcg cccaataatc tctccagttt cttagcatct cctgactcaa
       61 ccggaattat ccaagcccag tctaaataat tttttaaaat ctcatcatat tttgcaaata
      121 tttcattgcc atattccgtc cttaaaatta tattttcagt gccagacaag tgatttatat
      181 tgagttgata aaaaagatct ccatctaaaa aaatatcatt agaagaagat ttccccacag
      241 taatgaccat tccatcagct ctatgtgtta catacaaata attgtatgac ctcatttcac
      301 ctaatgaatc aattatttct tgccctttag ctctaatata ttgatctcta ttacctatct
      361 cactcttatt taacaaaagt tttttcatgt caaatttaat cggctgtttt atttttgtaa
      421 attcgcttct actactaatc caatccgaga ttaatttttc aacatgtccc agagtaaact
      481 ctgatttatt cgctaaatac tttttttgtt gtattaaaga catctttccc cgaagctcaa
      541 aattgagcct acatcgattc tctatctctt caaaaagcat cttatcttca tcattcttat
      601 attcatcatt cataaaacca atccagctcc tcgattaaga taatatttta atctcataca
      661 aaaactagct ctcatcaaaa caaccattgt tttgatttct catgaaattt ctttatactt
      721 ttctccgatt ataaattcct aaccccttga gaaaattacg catatattta tctccgcaag
      781 gcttaaaatt tcgttggcct tcttttctca aaaatgcccc gatttcgcct tttgatggat
      841 agatttctgc ctcagcaaaa acgtctaaaa tatcatcagt ggtcatagac aaggcgattt
      901 ttaatttttt tagcaacaca ttgttgatat atttgatttc cttgcttttt tgcggcgtgt
      961 ctactatcgg cttatctttt tgcggaccac gcgccaagag aatcaggcca tttaggaatg
     1021 cgtcaaaaac ttggttgttg atggtttttt catacacatt attttctgca ttatctttgt
     1081 tcttatcctg tactttggtc agtaccgcct gagcttcttc tttagtcagt ggtacaccgc
     1141 ctagctcaaa agctttaatc aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca
     1201 ataacctatc attgttattc atttgttcct ccgaagtatt tagtctttac tttcttacgt
     1261 actttttgtc ttgctaagat ttttaggatc aatgataaaa accgcatcac ttatgataag
     1321 gcgctgggta tcatgagtga atgatattgc tgaatacaaa tagtccgacc acattaagtt
     1381 gatcggactt cataagcatt ttattgacta atactttttt gcttaagaat tgattctaac
     1441 atcttttgct aaagttaagc atcctatagt tcctgcatta tctgctaact taggtgtgac
     1501 aatgtattct tcgatatttg gcgtatcgac atagccgtta attagttcta aaaaacgtct
     1561 ttgtactttc tttttcagat gttcttgttg catgacacca ccaccaaaaa ttataatatc
     1621 tggtgaaagc atcagggtcg tattacaggc acattgtgca atataatccg cctcgatttc
     1681 ccagaacgga tcttctatag aaagatcttg tcccttggtt cccgtacgca tctcgatagc
     1741 gggtccagat gccaaccctt cgagacaatc atgatgaaaa ggacaagtac cagagaaagt
     1801 atctcctgaa tgtctcttaa ctaacgtatg ccccatttct ggatggctaa acccttcaac
     1861 aaaaatccca tcttgtatag ctcctccacc aattccagtt ccaacagtgt aataaacaac
     1921 actggataaa ccttttccat gacctgaaac atactccccg taacaagcag cattcacatc
     1981 ggttgtccag gcaattggaa tttcgaaatg cttattcaac gttccaacaa aatcaaaatt
     2041 tttccaggca gtttttggag tagaggtgat atgtccatag gtctttgatt cacgatggat
     2101 atcaatcggt ccaaatgacc ctactccaat gctttctaat tgttctttat agtgattaaa
     2161 gaaatcaatt accaaactca ttgtttcatt cggtgtagtt gtaggaaaac tcactctttc
     2221 taaaaccttt aaatcatcat tgccaatggc acaaacaaat ttagtcccac ctgcttctat
     2281 tgagccatac atttcttact ccaccgtcct tttatctagg aattcgttct tccctgtcta
     2341 atagtttttc gaaaaactca tgtggttcct tttggtacca ataggcaaca gaagaaatat
     2401 cgtcttgacg ctcataaagc ttgatgtcat catttcctag ggtttggaaa gtaacacgta
     2461 aattttcttt gaaactaatg gggtcgggta aatgccagcg gtacaaccca tgcatcggta
     2521 aagcatcatc gccaaaacca tgaactggat ttggattagg ttttccagca ctaaaataat
     2581 cccgggtgtg atctctttta gattgaaaag gatagcccat aaatagcgta ttaaaatttt
     2641 gagcatatgc cctttctcca aagttctttt tatggaaagc ccaagcgccg ccaaaataat
     2701 cttctgctcc tgtagatgtt attgtaggga aatcttcatc tccatctata taaaacttaa
     2761 attctccttc cccccaccag tatctttcca atgcgcacaa agcaaagtag gttcctacat
     2821 aatacccagt gcctttgatt ccatcaataa gggtgtaatc ttttcctttt gtcgtgatgg
     2881 gttcacggtt ccatttagca tgaaaataca tcgcatcatc cggtacttca gtcaatgcat
     2941 aattaaatgt ataaaaaatg tgcttaataa actcaggatg ttcattcgtt aaagtaatct
     3001 tagctttttt tcgaaatggc atctcaaaat agcaattcat ccctgatgtt ggattcacta
     3061 cgattggtaa ggaattcact tcgcatcttg tcccaaaccc attacaaaaa aaatctccta
     3121 atggactttc tacagaagga gtggattcat catcccaata aatacgcaaa attaaatcac
     3181 gtaagacgaa actcccagcg attgttttgt ctgtcactgt catccaaaaa tgacgtaaaa
     3241 ttccagggcc ttccatttct gctaaagtaa ttgtctctcc agcatttatc ggaacagaag
     3301 gtgacccttt tctcccaatt cctagatgac tcgctgtcat tgccgctttc cctttttcac
     3361 catttttatt ttctggtgaa atagttctac tttcctctcc cttgtggagc catatattat
     3421 ttaaacaatt cattttattc gtcctctatc ctttcttcac ttttttgatt attcatatag
     3481 atgatttgtt gttgtgcctc cgcaataatt gctccaatat ctaaattatc atttcctaac
     3541 tgcaaagcga ttgacattgc caagggaaga ttcattcctg caattacgtg cactctgtca
     3601 gaaatatagg taaaaaactt ttgatttaca cttcctccag ccatgtcagt taaaacaatt
     3661 atttcgtctg tagtatccac taactcttta atttgttcat ctatatcatc aatttcttcg
     3721 acatatgcac ataagctact aatttgatta tgcatacctg ttaaaaactc tagtgtctct
     3781 ttaaaaccct cagctagtat tgaatggcta gcaatgatca atcttctcat aattcaaatc
     3841 cttttccttt acgctaatat tccaaatgca gccataacca tcgcaaaaac aataacgagt
     3901 agaatcaaaa tggtcatact gatttttttc ttactgagta gataatatgc tgcagcagta
     3961 ataattactg gaattaatgt tggtaaaatt ttgtccaaca ttggttggat tttcatacta
     4021 acttcaccaa aagtgaattt aagtggcgtt gaaacactga taactgaagc aattaatgat
     4081 ccaactactg ttagccccaa tactgaagca gcttcagtta aataattaat cttttcaccg
     4141 tattcagtaa caatcttcat tccctgctta taaccaaatt caaagcctct cattcggaaa
     4201 aagaagatta ccatgattaa aatgaaccat aaaaagattc ctacaggatt attttctaaa
     4261 gccatataag ccgcaattga tcccatgatg gttgggatca tcgtcatcaa aagagagtcc
     4321 ccaataccag caattggacc catcgtacca actttaaagt cattaatcgc atcttttgct
     4381 tctaatcctt gcttttcctc cattgcaaca caagcaccta aaataatggc tgccagccaa
     4441 ggctgtgtat tgtaatactg aaagtgatta tttaaagctt cgatgtattc atcatcatct
     4501 ttgtataatt ttcttaaaat aggtgacaat gcgtaagtca cagatgcacc ttgctgtgtt
     4561 tgataattaa aggtacaaac tgacatcaac caacgatagt tcgctttcct aagatccttt
     4621 ttcgttactt gatatttttc gctcatttta ttcatcctcc ccaataaacc caacatcaga
     4681 agctcccgca gaagcagtga tttgtgcatc tttttcagct ctatgataca cattaaatgc
     4741 taaagcgaat cctatgatcg ccgctccaat aataggtacc ttcaaaaaag cggctaagaa
     4801 aaatccaaca agtaaataac taatgtatcg acctgtaggc ataaagcgta acaacatcgc
     4861 aataccaaca gcaggcaaca tttttccggc taatgttagc ccatccataa accaattagg
     4921 taaaacatcc aaaacgtaat taacagcaga ctgtcctaaa gtaacggcaa taaatactgg
     4981 aattgctgca gataaaccaa agaaaattgg ccccaaccac aaaacactat tcattttctt
     5041 aaattctcgt ttattcgcat attcctgtga tttgcgaaca acaaaaccat ttaagatttt
     5101 gacaattaca tctagttgca cccctaacat acctacaggt agtcctattg ctaatccggc
     5161 ttctactcca agctggctac caaatgccgt tgcaataatt gcgcacgttc catagtccgg
     5221 tacagatgag ccaccaaaac cggcaactcc taatgacatc aattgaactg taccgccaac
     5281 tatcaaacct gtttttatat cacccatcaa aattcctgtg aataatcccc aaaaaactgc
     5341 gtaatttaca aagatcatcg gaatcccata ataatcaaca tgtttaataa atgctaatat
     5401 agtcagcata ataatttgaa aaatagtaaa atgcattatc cttactcctt tatcctaatt
     5461 ttgattgaat ttcctgtgcc ttatccgccg gaacaaattg tgtaacaatt tctacaccag
     5521 aatccaacaa ttttttatac gtgctgatat tttcatctga tgcataggct ctagaagata
     5581 gctgtaatgc attatcttta acaacagtac cgccaacaat tagtcggtca attctaatac
     5641 cgttatctac taaatgaagg attgtttccg gctctttcac gatgacaaat actttttcct
     5701 gatcgtattt ccccttttta aaattcgcaa tcgctgtttc ttcagtgata attgatacag
     5761 ccattccagc aggtctagct aaacgcatac ttgatttttg gatgtcgtta tttgcgacag
     5821 aatcatcgat caccatcact ctgtttgctt tagacattgg tgcccattgc gtgacaataa
     5881 tcccatgcaa taaacggtta tcaactctcg acataacaat actcataaat acttcctcct
     5941 ttatctttca ttttcttaca agcaattacc atgccaaaac aaaaagaact tgataaatca
     6001 agctcttttt acataatcat aaacgtattt aacctcatga atcgggatgt cgacactata
     6061 ataatgttca acgacactga aagattgctt aaaatgtgtc tgaaattttt gaaattcctc
     6121 actcgtttca ttgaaataaa tttcatcttc caaagtattt tgttttacta atcgttcaat
     6181 taagcaacta atgtgaacat ataatccgaa acacgtatta ttatcaaatg aaattcctag
     6241 atcattttgt aagatgtata tagcatctga aacccgttct aataatttag tcggatttaa
     6301 tatggtcaat tgctccatta aattcgttag actaaaattc ttcagaatat tattgttcaa
     6361 aactttcagt tcattatc
//
LOCUS       DARBER010000132         6303 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DARBER010000132 DARBER010000000
VERSION     DARBER010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.136"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            334..714
                     /locus_tag="SCK06_002422"
     CDS             334..714
                     /locus_tag="SCK06_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1152374.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="SCK06_002423"
     CDS             696..1610
                     /locus_tag="SCK06_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1152375.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="SCK06_002424"
     CDS             1855..2544
                     /locus_tag="SCK06_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152376.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="SCK06_002425"
     CDS             complement(2665..2751)
                     /locus_tag="SCK06_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1152377.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="SCK06_002426"
     CDS             3427..4377
                     /locus_tag="SCK06_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG1152378.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="SCK06_002427"
     CDS             4398..4931
                     /locus_tag="SCK06_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1152379.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="SCK06_002428"
     CDS             4947..5117
                     /locus_tag="SCK06_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152380.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="SCK06_002429"
     CDS             5567..6100
                     /locus_tag="SCK06_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152381.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DARBER010000133         6237 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARBER010000133 DARBER010000000
VERSION     DARBER010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.137"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            176..1021
                     /locus_tag="SCK06_002430"
     CDS             176..1021
                     /locus_tag="SCK06_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1152382.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1302..2522
                     /locus_tag="SCK06_002431"
     CDS             1302..2522
                     /locus_tag="SCK06_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG1152383.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2519..5239
                     /locus_tag="SCK06_002432"
     CDS             2519..5239
                     /locus_tag="SCK06_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1152384.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5223..6167
                     /locus_tag="SCK06_002433"
     CDS             5223..6167
                     /locus_tag="SCK06_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1152385.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2328 a          972 c         1208 g         1729 t
ORIGIN      
        1 tggattcaat tcatatttat ttataaatca aaaatacaga gaatagtata taaatgtcct
       61 ttcctattct taaaatgatg tttgtactta cttaaattag gtataattag tatatgtttt
      121 gtgagaaatt tagacttaca ataagcagtg aatttataat caacgtatag gagacatgga
      181 tgaaatgaaa ctaattggta tagatttaga cggtactctt ttgaattctg accagagcat
      241 tagtcaaaaa aatgcaagaa caatgaaaca tttaccggaa gactgttttc cattcatttg
      301 ttctggaaga gaagtagaag acattaataa tattctcaaa aaatctggtt taaggatacc
      361 agcagtaggg ttaaatggtt ctgttggatt tgatggagaa aaaaagatat ttgagtcttc
      421 cttcgataaa aatgatgtaa aaaaaattga ttctgtccta tcgaaatttc caaccaaaat
      481 atatacaaat ctcggaagtt atgaatctca gaattataaa aataaaatga aaaaaatttt
      541 tcacgaggta gggagtgaat tttctatcaa agagctaaac tatgagcttg agtatgaaaa
      601 atctatttgt tcaatccctt ataataatat tgaggaaatt atagaaaagg atgctatcag
      661 catttacaaa ttttttattt ttattcctaa tcgtcaattg aaagctaata tatttaacca
      721 tttgaaaaag atgctaaatg tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt
      781 acctcaaaat gtatctaaag gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa
      841 gaaacctttg agaatcgcga tcggagatag tttaaatgac ttatctatgt ttgaaagtgc
      901 agatattagt tttgcaatgg gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt
      961 cactgtttca aatgataaag acggagtatc tcaagcattt gaaagaattc ttaccatata
     1021 aactaattaa ggtagtatag aacatagaat tctaaatacg gaaaaatgga gcataaattc
     1081 cagtacaaat agtacttctt cagtgtcaaa cgaagcgaat ataacataga tatttaggat
     1141 tgtgacaaaa atcatgtcac aatctttttt tcgaataaac ttattttttg aagtcggcca
     1201 tttatcgtaa tttctttgca taatacgaac aaattttcgg ttattgatga aaaaaaacat
     1261 tgaaaatggt atgataaaag aaaaaaagga ggaaattttt aatggtgaaa tttatccatg
     1321 cggcagattt acatatggat cggtcatttg aaggactgac aactcttgat aaaactgtac
     1381 aggaaaaact attgaagacg aatctgaccg ttttatcaaa tatcattgaa caggcaataa
     1441 taaataacgt tgattttgtt ttacttgcag gagataattt tcatcaaaat cgtccttcat
     1501 taaaaataca gaaacatttt tcagaacaga tgaaacggtt agaaaagaac catatacctg
     1561 tatttataat attcggaaat catgatttct atcaaaaaga gcggtattgg ttctcctttc
     1621 ctaaaaatgt ccatttattt acatccgaaa cggtagagac aaaaaaaata acgataaaaa
     1681 gtggagaaac tgtctctctc agtggattta gttatcgtca accgtggatt caaaaagaca
     1741 aagtgatgga atttccttct agagaactca cagattacca tattgggttg taccacgggg
     1801 aacctggcgt ctctcaaaaa ggaaactatg ctcctttcca accctcaaag atgcaagaaa
     1861 agggatacga ttattgggct ttagggcata tccatgtgcc aacggtctta aatgaaaagc
     1921 aaacaattgt ttatccaggt gcgcctcaag gtcatactaa aaaggaacaa gcttctacat
     1981 ctattctact agttgaatta agtaaaggaa gttgccaaat acatccgatt aaagtagcag
     2041 aggtctattg gaagacaaag gaaatatcat tgaggaaagc aagaacgaca aaagaggtca
     2101 ttgttcacat tcgtcagcag ctttcaaaag tagaggatgg atttagttta attgagataa
     2161 aattaaaaga ttatgaccat ctcaatacag atgttttgga acggatacaa tcaggagagc
     2221 tattagacta tttgttagag gaattctcag ataggataaa tgatttcttt atttggcgta
     2281 tttctctcat agagaatact ttttatacga agaccccact tgctgcttct gctaaattga
     2341 tggaacagtt atttcaatac tatcagacac ctcaagattt ccaacaaatt ttaaatgaag
     2401 tatatagtca tcaagaagcg gctagaatat tgagcgaact gccggaatat caagaagaaa
     2461 cacttgaaaa agccaagcag ctgcttaatc aagatttttt atttgaggag gatcaggaat
     2521 gaagatttta aggatcgaga ttgctgcttt tggaaaatgg cgtcaaaaaa gttttgactt
     2581 ttattcaggc aatcagctga tctatggggg aaacgaagca ggaaaatcaa cgatttatca
     2641 atttattcaa gcaatattat ttggtttccc ttcaaaaggc cgtaaaaaaa aggattatac
     2701 accaaaagac ggctcagcct atggcggcaa aatatggctt aaacatcctg tatatggtga
     2761 atttgctgtc gagcgctaca agcagcaaaa tcgcggaaaa tcgaaggtgt ggctggggga
     2821 tcaagtggga agtgatgagt tactagaaaa actgatcgca cctttgacga aagagttatt
     2881 ccagcaggta tttactttcc agcaagagca gttgatggaa ctagatcatt tgagagaaaa
     2941 agagctacat gatgcgctga tttctttagg catcacgggc agttctcaag tattccaaaa
     3001 gcggcaagaa tattatcaga aagcacaaca attattcaag aaaaaaggcc aaaaattact
     3061 aatcaatcaa aaattaaatg aatggaaaga gctgcaagaa aaaattcatc aaaaacaaga
     3121 acaggaagct tattttggca agttgatcca acaagaacag gaatacgagt cgcaacagca
     3181 gcagttattg gcacaattaa aagaggcgaa caaacaattt tcattagcca gacagcaaga
     3241 acttaatttt tctttatatg aagaatggca atctctcaaa aaaaacagca ctacaggtct
     3301 tcctgatgag caacttcttt tagatctgga gtctttctcc aaacggtatc agcaaacaaa
     3361 tgagcgtttg gaagaattag aagcaacact ggaaaagcat agcgggatgg atcagcagtc
     3421 agccagatat tatttttatt tagagcagga gcaggaacta aaagatctcc agcaacttca
     3481 atttacagcg gaacaattaa acgaagaatg gcaaagatta caaattgaga ctagagaaac
     3541 aaattcagaa ttgaagattt tggaacaaag gtggcattgg cagaaaaatc cgttaccgga
     3601 agaattttta tatgaagaag aatggaagca tcttctggct gaagaaagaa ggattgctga
     3661 tctagaaagc cagatttctg tgcgtctcca aatagctaaa gaacagcaag agatgattga
     3721 agcagaagtg aatgacttgg aagagaccta tccagcatta ttacaaaatc agtcagagat
     3781 agcaccagtc tcttcatcta aaccgtatat gctgatggct ggcggagcaa taatcattgc
     3841 aggtatattt gtaccaatgc ccttgaaagt gatcctttta ctaattggtg caggcatgat
     3901 tggctatttc ttctacaaaa ggcaaccaaa aattaaaact aaaaatgatt tctctgagat
     3961 aaaaggaatg tggcaagaaa aactaggaca acttgatcta gccttagctc aattagctga
     4021 agcagaagaa aaacaaaaga gaactggaga agaaaaacag attcttcagc aagaagtata
     4081 tcgttttgcg gatcatcggc acttaggaaa gatggatacg ctcaaactca tgaaagaata
     4141 tggacaggaa gtcatagatt atcagaaatt atatggcgta tttgagcgga aacataaaag
     4201 agaacaggaa atacaagaaa aactacgaca aatcgaccaa aaatttgagt ttgttaaaga
     4261 atggttaccg atacaagata agacaatttc agaaaaaatg gtattgcttt cccgttttat
     4321 acaagagatg gaacagatga aatttgccag aagctatcag caaaatactt tattgaagca
     4381 agaaatcaat caattgaaaa agaaacaaaa agtatggata caagaataca gcgaacaatt
     4441 gactcaagca ggcttatctt atccttcgga agtacctgtc tttttacaaa aagccaaaca
     4501 agccgaacag cagatgaaaa gatacaatga attatcggaa atgcttactc cactatttcc
     4561 tgaagagctg acaaaagaga aattatctgt aagactgcgc caactgaaag aaaagcaatc
     4621 ccaactccag caagaagaaa aagaagcaag tgaaaatcgg caacgactcc aacttcaagt
     4681 agaagagttg caaagagacg gcacattgga cgaactttat caacaagcta gccggcaaaa
     4741 agcaaaatta caggaattac tggaggaatg gggagcactt gaaatcgccg ggacattctt
     4801 aggtgatctt tcaacagaga tgagtgaaag acaattaccc cagttgttaa gagcagcagg
     4861 tgattattta ggtattttaa caaaaggaaa ttatcagaag atattactga aaaatgatac
     4921 actgacttta acagacggat atacagaatt tcccatctat gagttatcta caggtacgaa
     4981 agatcagctg attatggcga tgagatttgc ttacctctca ttagaagcta accgttcgat
     5041 atgcccgatc atcattgatg atggctggct tcattatgat catcagcgaa agtatcaatt
     5101 agctgaactt ttagctaaat ttggagaaaa gtatcaaata atttgttttt cttctgataa
     5161 agaaatggta agctattacc atgagatgaa ccaaatcgta aaacaattag aaggagcaaa
     5221 taatgaaaaa aattcgtgat ttagtaatcg atgagcagtt tgaacaattt gtcctgatca
     5281 aaagtgcaga tgtaagaatc gctaaaaacg gaaaaaaatt tattgctttt actttccaag
     5341 atacttcagg tactatcgat ggtaaatact gggatgcttc ggaggaggaa atcaataaat
     5401 ttacagccgg cacagtcgtt tttctaaacg gtaaaagaga ggtttatcaa ggaaaccctc
     5461 aagtaaagat tcttcatatg cgcttaacaa gggtagatga gccaggagag cctagtttat
     5521 acatggaaag agcccctttg aagaaagaag aaatggaaga agaaatcaat caattgttgt
     5581 ttgagatcac caatgcgcat tggaatcgta ttgttcgcta tctacttaat aaatatcaaa
     5641 aacaattttt tgagtttccg gcagcaaaaa gaaaccatca tgcatttgcc agtggattag
     5701 cttatcatac tttgacaatg cttcacttag ggaaatcaat cgccgatgaa tatcctgagc
     5761 tgaatcgagc attgttgtat gctggtatca ttttgcatga tttaggaaag gtgattgaac
     5821 tttccggtcc tatgtcgaca gaatatacgc ttgcaggaaa tttgatcggt catctggtat
     5881 tgattgacga agaaataact aaagcctgtc ttgcgttgaa aatcagcgaa gctgacgaag
     5941 atgttctcgt attacgtcat atggttttag cacaccatgg cttattagaa tatggctcac
     6001 ctgtacgtcc aagaataatg gaagcagaaa tattacatca aatcgacaat atcgatgctt
     6061 cgatgcagat gatgttgact tcgattcgtc aaacagaacc aggaaaatac accgatcgga
     6121 ttttcggtat ggacaaccga agtttctatg taccaaaaga tttatagcaa aaatttattt
     6181 ttttcattca aaacaaaaaa agcgagacat aagtcttcgc tttttttgtt ttctttt
//
LOCUS       DARBER010000134         6173 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARBER010000134 DARBER010000000
VERSION     DARBER010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.138"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            725..811
                     /locus_tag="SCK06_002434"
     CDS             725..811
                     /locus_tag="SCK06_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1152386.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="SCK06_002435"
     CDS             complement(932..1621)
                     /locus_tag="SCK06_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152387.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="SCK06_002436"
     CDS             complement(1628..1906)
                     /locus_tag="SCK06_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152388.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="SCK06_002437"
     CDS             complement(1866..2780)
                     /locus_tag="SCK06_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1152389.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="SCK06_002438"
     CDS             complement(2762..3142)
                     /locus_tag="SCK06_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1152390.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="SCK06_002439"
     CDS             complement(3592..3879)
                     /locus_tag="SCK06_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1152391.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="SCK06_002440"
     CDS             complement(3899..4123)
                     /locus_tag="SCK06_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG1152392.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="SCK06_002441"
     CDS             complement(4578..4748)
                     /locus_tag="SCK06_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152393.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="SCK06_002442"
     CDS             complement(4763..5296)
                     /locus_tag="SCK06_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1152394.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="SCK06_002443"
     CDS             complement(5317..6159)
                     /locus_tag="SCK06_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1152395.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DARBER010000135         6146 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARBER010000135 DARBER010000000
VERSION     DARBER010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.139"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..2542
                     /locus_tag="SCK06_002444"
     CDS             101..2542
                     /locus_tag="SCK06_002444"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG1152396.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="SCK06_002445"
     CDS             complement(2872..3576)
                     /locus_tag="SCK06_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152397.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="SCK06_002446"
     CDS             complement(3763..4008)
                     /locus_tag="SCK06_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152398.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="SCK06_002447"
     CDS             complement(4025..4876)
                     /locus_tag="SCK06_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1152399.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="SCK06_002448"
     CDS             5042..6046
                     /locus_tag="SCK06_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG1152400.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1970 a         1064 c         1301 g         1811 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtg
//
LOCUS       DARBER010000136         6109 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARBER010000136 DARBER010000000
VERSION     DARBER010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.140"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            280..969
                     /locus_tag="SCK06_002449"
     CDS             280..969
                     /locus_tag="SCK06_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1152401.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            956..2164
                     /locus_tag="SCK06_002450"
     CDS             956..2164
                     /locus_tag="SCK06_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1152402.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2458..3729
                     /gene="serS"
                     /locus_tag="SCK06_002451"
     CDS             2458..3729
                     /gene="serS"
                     /locus_tag="SCK06_002451"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG1152403.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4278..5762)
                     /gene="guaB"
                     /locus_tag="SCK06_002452"
     CDS             complement(4278..5762)
                     /gene="guaB"
                     /locus_tag="SCK06_002452"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG1152404.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1873 a         1152 c         1195 g         1889 t
ORIGIN      
        1 tatcaacatc acatgtgaaa gtcaataaga aaatgcttat ttatcaacat ttctagctta
       61 catgatcatt ggttagttct ggtccttttt cgttcgtctt tataactccg agagacgtaa
      121 aatccttttg ttttcagttt aatgttcatg ttcttgtctt attcacacca actagatgtc
      181 tcaccttcta aaattttcct taacaaagtg ctgcttttat ctatttctgc ttttttttgc
      241 tagaataata gtaacaatat gactctggag gatttattta tgaaaatttt gattgctgac
      301 gatgataaag aaatcgttga attattaagt atctatgttc acaatgaggg gtatgaagca
      361 gtaaaggctt atgatgggaa agaagcatta tctaaaatca gaacaatccc tgatattgaa
      421 ttacttattt tagatatcat gatgcctgag atggatggga tgcaagtagt gaaagagcta
      481 cgaaaagaat cccaaatacc aatcattatg ctgacagcaa aaactactga tatggataag
      541 atcaaaggac tggtcgctgg tgcagatgat tatgtgacca agccatttaa tccgttagaa
      601 gtaatggcta gagtaaagtc tttattacgg cgtacaaaaa tgcagattgc cccagatcag
      661 cctgatgtat tagaagtaaa tgcattaatc atcaataaag actctcatga agttaaaaca
      721 atcgatggga aagaaatcca gttaacagct ttggaattcg gtatcctcta cttgcttgct
      781 agtcatccta atcgagtatt tagcgcggat gaaatttttg agcgtgtttg gaaacaagaa
      841 agtattgtct ctgctaaaac tgtcatggtt cacgttagtc atttaagaga taagattgaa
      901 gaagcaactg gtggtgaaaa agtcattcaa accgtttggg gagtgggcta taaaattgac
      961 gcaagatagt aaacgagcaa ctgaaaaacg ccgtcggatc aatttgactt caaaagaaat
     1021 cagcgaattg ttagcggaag gaatcatcac gattatcctt ttgcttctat taaatgtttc
     1081 tattctggtt gttatcagtt cggtgatcaa cagttcacct tctttgacga atgcgatttg
     1141 ggattcaaag aatatttttg cagaacgatt gaacactgat ttgttctgga acggccggaa
     1201 ttttattatt ccctttttct ttctattaga tattggtgtt ttgtattggc gcttgattcg
     1261 aagatatcgc caaatgcaat tgcggcatat catcagcgaa cttcactaca tcgcaaacgg
     1321 aaattatgat catcgtatcc cttttgaact gagtggtgac ttaagccgag tggtaacaag
     1381 tatcaatgga ttggtggata gtaccgtagc agcgattgaa gacgaacgta aaatcgaaaa
     1441 atcaaaagat gaattgatca cgaatgtcag tcatgatatc cgtaccccac tcacgtccat
     1501 cattggatac ctaggactga tagaagatgg ccagtatcac tcagaagaag atcttttaaa
     1561 gtatacgcat actgcttata tcaaagcaaa gcagatgaaa tctttagtgg acgacttatt
     1621 tgaatacact aaggtacgcc agcctgctgt cccggtgaat ttttctgcct ttgacatgat
     1681 ccagttgatc gaacagttag cagctgattt tgagttggaa gcttctaaaa aaaatatcca
     1741 aatacttgtt caatcaaaag tagactccct tatcatggat ggcgataccg aaaaacttgt
     1801 acgtgtcttc aataatcttt tgacgaatgc gttgaaatac ggaaaaggag caacgaagat
     1861 cgtaatcgaa gtagaacgta tcggttcaga agttgttgca acagtcaaaa ataacggagc
     1921 aatgatccca caacaggcaa tcgataatct attcgaccgt ttttatcgtg tagaagaatc
     1981 tcgttcacaa gcaactggag gaactggact tggtttagca atcgctcaaa gcattgtggc
     2041 tttgcacggc ggatacattt atgcaaaatc agataagcaa tggacttctt tcatcatgca
     2101 ccttccaatc aagaaaaatg aaaaattacc tattgataca caggaagtta ttgacgaaag
     2161 ctgaaatatt cgctattctt attacaatta ttagaataaa tgaaaaacac agaacccagt
     2221 acactttatt ctttttcaga gagctgatgg caggtgtaaa tcagcaaagg ataatttgga
     2281 agttccagtt cgtttacgtg ccctttcgaa ataagaaagg tcggttcaac agccgttatg
     2341 tgttcaacaa gtgcagctga cgctgaactt gggtggtacc gcgaatttca gactgattcg
     2401 tcccaagaat aatgggatgg atcagtcttt ttttattttc aaaaaggagg aaatcatatg
     2461 ttagatgtaa aaatgatgcg acaaaatatt gaagaagtac aagaaaaatt gatgactcgg
     2521 ggcgtaaaaa aagaaacatt agctgaattc atggaactag atgaaaatcg tcgtaagcta
     2581 ctagtcaaaa gtgaagaatt aaaaaaatat cgaaatgatg tttctgctga gatcgcccaa
     2641 ttgaagcgtg caaaagaaga tgcttctgct aaaatcgctg aaatgaaaga agtcggtggc
     2701 aacatcaaag cattggatac tgaaattgct gaagtggatg aaaaattgaa acaaattgca
     2761 actactttgc caaaccttcc ccatgattct gtgccagttg gaacagatga agaggacaat
     2821 gtagaaatcc gtcgttggag cgaaccacgt acatttgctt ttgaaccaaa accacactgg
     2881 gaaattgcag aaaatcttga tattttagat tttgaacgtg gcgcaaaagt agccggtagc
     2941 cgttttgtct attacaaagg attaggtgct cgattagaac gtgctgtcta caacttcatg
     3001 cttgatcagc atatttacga acacggctat actgaaatga tcactcctta tatcgtcaac
     3061 agtaaagcga tgtttggtac gggacaattt ccaaaattca aagaagatgt tttccaattg
     3121 gagaatactg atctaacatt aatcccaaca gctgaagtac cattaactaa ctattataat
     3181 gatgaaattt tagatggtgc agatttacct atttatttca ctgcactaag ccctgctttt
     3241 cgttctgaag ccggaagtgc tggtcgggat acacgaggac tgattcgtct tcaccaattc
     3301 aataaagtag aaatggtcaa attttcggat gtagagcatt cttacgaaga attggaaaaa
     3361 atgacagctg atgcagaaga tatcttgaaa aaattgcatt tgccttatcg tgtgatggcc
     3421 ttatcaactg gagacatggg cttttctgca gctaaaactt atgacttaga agtatggata
     3481 cctgcacaag atacttatcg tgaaatcagt tcttgttcta actgtgaaga tttccaagca
     3541 cgccgcgcaa tgatccgtta tcgcgataat gatggaaaaa tccactatac tcatactctg
     3601 aatggttcag gacttgcagt tggccgtaca gtagctgcca ttttggaaaa ctaccaaaat
     3661 gaagatggtt ctgtcacagt tccagaagcg ctcgttcctt atatggggaa tctgaaagtt
     3721 attaaataaa gaggctgtga aaaagccgct tagtttcgag tattaagaaa aattttgtga
     3781 aaatggcttt ccacattttt gcataaattg gacttaatac cgagaaactg gcttttgaac
     3841 accatttatc gagaggttgt gaaatcagcg aattaacctg agtattagag aaaaaaatgg
     3901 aaaaatagtc cctcatattt taccgtttgt tgaactaata ccgagggtca gctgattgaa
     3961 caccgtttag tggagaggtc gtaagcctac cccccagctc ctagttacaa ggacaaattc
     4021 aaaaaacagc tcttcatatt ttatgaatgt tgtacttgtg attcggaggt ggcaagggaa
     4081 cactgtttat tcaaaaagag gctgggacag aagcgtttaa ctccaagaaa taagaagaaa
     4141 ttcacgaaaa ttgcttttca aatttttgtg aatttcttct tatttccgaa ggagttgctt
     4201 ctacttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc
     4261 cacgctcttt aatgttttta agattcaact gaatagtttg gtgcttcttt tgtgatttgt
     4321 acatcatgag ggtgtgattc ttttaatcca ctgccgctca tttggataaa ttgcgcttca
     4381 tcacgtaatt gctgtagatt ggctgcccca acataaccca ttcctgaacg caaaccgccg
     4441 atcatttgga aaataatatc tgcaacactt cctttataag caacccgacc ttcgattcct
     4501 tctggtacta atttatttgc ttcattgaca ctaccttgga agtaacggtc ttttgaacct
     4561 ttttccatcg cgcctaatga gcccatacct cggtatgttt tgaatcgacg tccttggaag
     4621 atttcgaatt cacctggtga ttcatctgtc cctgccaaca tacttcctag catcactgca
     4681 tgtccgccgg cagctaatgc tttgacgatg tcaccagaat atttgatccc tccgtctgca
     4741 ataatcgctt tgccatattg acgagcaact gatgctgcat cataaatagc tgtgagctga
     4801 ggtacaccta caccggctac cacacgtgtc gtacaaatcg atcctggtcc gataccaact
     4861 tttacgacat ctacacctgc atcatataaa gcttttgtcg cttcagccgt cgcaacgttt
     4921 cctgcaatca atgttgcatc tgcaaaagtt gaacggattt cttgaatttt tcggatcaca
     4981 cctgcactat gtccatgagc tgtatcgatg atgattgcat ctgcccctgc ttctaataaa
     5041 gcattcgcac gctcaaatgt atcgcttgtt acaccaacgg ctgctgctac aagcaatcga
     5101 ccatgttcat cttttgctgc atttggaaat tcgatcactt tctcgatgtc tttgatcgtg
     5161 atcaatccgc ttaaacgacc ttcgttatct acgattggta atttttcgat tttatgtttt
     5221 tgcaaaatct tttctgcatc ttttagagag gtacctactg gggcagtcac taagttgtct
     5281 tttgtcatca catcagcaat cggcatgtga taatctgtta caaaacgcat gtcgcggttt
     5341 gtaattatcc ctaccaattt tctgttttca agtgtttcaa cgattggcac accactgata
     5401 cgatatttgc tcatcaattc ttctgcgtct gctacaagat gctgtggcgt taagaagaac
     5461 ggatcaatga ttacgccact ttcagaacgt ttgacttttc ttacttcatc cgcttgctgg
     5521 ctgatcgtca tgtttttatg gatcacacct agtccgcctt gtcttgccat agcaatggcc
     5581 atcttgctgt cggtaacagt atccatactt gcactgatga ttggaatatt caacttaata
     5641 tttttagcta attgtacgct catatccact tcgtttggta acacgtgact ttctgctggt
     5701 atcaataaga catcatcaaa agtaaatcct tttttcgtga actttgtttc ccagttggac
     5761 atttttagta ccactcctct aatttagttt atctattaaa ataattgatt ttttcattta
     5821 agtcaatgat ctacttggaa tcaagttagc tttctcatag aataccaata taaagcgttt
     5881 acatctgtgt atattaaaag agattgtaaa aaagctgtcc tgcatcaagt aataagaaca
     5941 gccaatcaaa aacagcttct catgtttttg aattggttgg gtttattacc gcagatgcaa
     6001 acttttgaac accgtttagt ggagaggctg tgaaaaagct gcttagtttc gagtagtaag
     6061 aaaaattttg tgaaaatggc tttccacatt tttgcataaa ttggactta
//
LOCUS       DARBER010000137         5910 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARBER010000137 DARBER010000000
VERSION     DARBER010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.141"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(136..594)
                     /locus_tag="SCK06_002453"
     CDS             complement(136..594)
                     /locus_tag="SCK06_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1152405.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(607..1938)
                     /locus_tag="SCK06_002454"
     CDS             complement(607..1938)
                     /locus_tag="SCK06_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG1152406.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1949..3391)
                     /locus_tag="SCK06_002455"
     CDS             complement(1949..3391)
                     /locus_tag="SCK06_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1152407.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3378..4142)
                     /locus_tag="SCK06_002456"
     CDS             complement(3378..4142)
                     /locus_tag="SCK06_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG1152408.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4246..5121)
                     /locus_tag="SCK06_002457"
     CDS             complement(4246..5121)
                     /locus_tag="SCK06_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1152409.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5376..5460)
                     /locus_tag="SCK06_002458"
     tRNA            complement(5376..5460)
                     /locus_tag="SCK06_002458"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5424..5426),aa:Leu,seq:caa)
     gene            complement(5478..5549)
                     /locus_tag="SCK06_002459"
     tRNA            complement(5478..5549)
                     /locus_tag="SCK06_002459"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5515..5517),aa:Gln,seq:ttg)
     gene            complement(5559..5631)
                     /locus_tag="SCK06_002460"
     tRNA            complement(5559..5631)
                     /locus_tag="SCK06_002460"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5596..5598),aa:His,seq:gtg)
     gene            complement(5693..5777)
                     /locus_tag="SCK06_002461"
     tRNA            complement(5693..5777)
                     /locus_tag="SCK06_002461"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5741..5743),aa:Leu,seq:caa)
     gene            complement(5798..5868)
                     /locus_tag="SCK06_002462"
     tRNA            complement(5798..5868)
                     /locus_tag="SCK06_002462"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5834..5836),aa:Cys,seq:gca)
BASE COUNT         1811 a         1257 c          900 g         1942 t
ORIGIN      
        1 cataggagca acaaaaaaat tcgataatta acaaaaatca aaatgattaa atctagtgat
       61 tgaaacatat catttcaaaa aaattttgta aattcaccca taaatatcac cctatttaac
      121 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
      181 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
      241 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
      301 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
      361 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
      421 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
      481 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
      541 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
      601 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
      661 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
      721 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc
      781 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt
      841 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
      901 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
      961 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
     1021 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
     1081 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
     1141 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
     1201 ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat
     1261 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
     1321 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
     1381 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
     1441 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
     1501 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
     1561 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc
     1621 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
     1681 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
     1741 aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt
     1801 tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt
     1861 caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat
     1921 ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg aattctgcca
     1981 taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga
     2041 tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct
     2101 ctacccgtag gcgtgtatta caactataga catccaggat attggctttt cctccaaacg
     2161 cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt
     2221 cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt
     2281 ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga
     2341 ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac
     2401 atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac
     2461 cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa
     2521 caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga
     2581 ccatctcttt attttcttta taagctgctt tatagatagc tagacatata gctggtatac
     2641 caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga
     2701 taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat
     2761 cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca
     2821 aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat
     2881 caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta
     2941 gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta
     3001 atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac
     3061 caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa
     3121 tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg
     3181 caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa
     3241 ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca
     3301 tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca
     3361 tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt
     3421 ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac
     3481 acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg
     3541 atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta
     3601 ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa
     3661 tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt
     3721 tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg
     3781 tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc
     3841 tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac
     3901 tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt
     3961 aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa
     4021 cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata
     4081 atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc
     4141 atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt
     4201 actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc
     4261 tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc
     4321 gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt
     4381 aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa
     4441 tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata
     4501 ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa
     4561 actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt
     4621 tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc
     4681 atattcattt ggataaagga atctcatttc tggcaacata attgctgtga catctctatc
     4741 ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga
     4801 atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat
     4861 aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc
     4921 taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa
     4981 gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt
     5041 atgattcagc tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa
     5101 acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa
     5161 cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacataa
     5221 tttacaaaaa tagtttaaca cataataaaa gcgttatcaa tatctttttt actcgtttta
     5281 tgataatact tgtatcgaat tgatttggag gtaattacat aaaaacaaaa aaaatatagc
     5341 aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc
     5401 acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
     5461 aaatttaaaa ataataactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt
     5521 cccttgcctt accgcttggc tataccccaa gaataaaggg cgaatgatgg gaatcgaacc
     5581 cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatcataaa
     5641 agatgttatt taatttctca ataaaaaact aagatgaaga catcttagtc aataccggcg
     5701 gccggggtcg aaccggcacg ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc
     5761 aattccgcca cgccggcaag ttgatttatt agtactaagg cggtaaccgg atttgaaccg
     5821 gtgatgaagg ttttgcagac ctctgcctta ccacttggct ataccgccat tatactatta
     5881 tcaatctata aaataaagaa taaattatag
//
LOCUS       DARBER010000138         5826 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DARBER010000138 DARBER010000000
VERSION     DARBER010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.142"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(480..983)
                     /locus_tag="SCK06_002463"
     CDS             complement(480..983)
                     /locus_tag="SCK06_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1152410.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1342..2367
                     /locus_tag="SCK06_002464"
     CDS             1342..2367
                     /locus_tag="SCK06_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1152411.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2690..3568
                     /locus_tag="SCK06_002465"
     CDS             2690..3568
                     /locus_tag="SCK06_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG1152412.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4044..4160
                     /locus_tag="SCK06_002466"
     CDS             4044..4160
                     /locus_tag="SCK06_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1152413.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5088..5426
                     /locus_tag="SCK06_002467"
     CDS             5088..5426
                     /locus_tag="SCK06_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152414.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5520..5750
                     /locus_tag="SCK06_002468"
     CDS             5520..5750
                     /locus_tag="SCK06_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152415.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1978 a          878 c         1074 g         1896 t
ORIGIN      
        1 ataacgacac agaaaatacc gtgtctcaga tttctctact acttctaaaa actactgttt
       61 ttttcctttg aaatgccttc aaaaagtagg atcaggcaga atatactggc agaatcaagg
      121 agaagggagc ctaaataagg agaaaaacca tagaaaatat aaagtaatta cttataaaac
      181 aagcttttta tagaaaaata atatacctga gtagggtata aattaagacg gaattccttt
      241 ctagattgag atattgacta ggaagaatgt taagatttta agcctttaag ctaaaaaaaa
      301 accctttttg ttaaataagg ggaatatagc ctgtcagttt caaactgcaa gtcgtcctgc
      361 tgcaataata tagtagatta gagatgaaaa tagatttttt taaaccaatg actgaataaa
      421 aagaccgtca tagatacaca ttggaatgtg tctattacgg tcttttttta cgaacgattt
      481 tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta cgatcagaaa taagaataaa
      541 atgtatccca aaataatggc tagacggtct aacttatttg tcgatagatg tttgtttaga
      601 agttgatagg cccataaacc tatcagtcct cctgttgtat tagttatcaa atcagtgatg
      661 tccgctacgc caataccgaa taggaattga atcaattctg ccaagaagct tacaaggaaa
      721 ataatcccta attttttaga aaaagaaagt ctcttgaaat tgacattcaa aagcacccca
      781 aaaggaaaaa agacaatcaa gttataagtg gtctcacgta ataccgtcgt attttcaaaa
      841 gaaaaagtag aaaaagggac gagattgaca ttgctgtaag aataagtgag aaattgtgga
      901 agattaaatg atagcttgaa taaaatcaac cataatagaa agaataaata aatccctaat
      961 aaaaattttg atacataccg catagacatc tcctccattt aaaaagtata ccatcgaaaa
     1021 ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca acgtagttat cgtacgaaaa
     1081 ttagaagctt ttttttgttg ggaatccggt ttctttaaca aagctaagat agcgagttgc
     1141 ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg aatgttcgtt ttttgttcta
     1201 atttctccaa gttgtttact ttttgatact aagtttatga aaaatcaaga aatagttgtt
     1261 atactaggta atgctgacaa acgggattgg catttcatct tggttcagct ttttttccaa
     1321 ttactaggaa ggagggatag attgttcaag acttattttc gtttcaataa aatacaagat
     1381 aatccttttc gtatcatgaa tctgatgcta cttatcttta ttgtattatg gttaggttac
     1441 ataaatcatg attttttgat ttcaagtttt tcatcattag ggatctttac ttttttctat
     1501 tatcaaaaca taccgatgaa atatttgctg aaacgtatgt ttctgatcgg ttgcggactg
     1561 ttggttgctt tcgttttggg gatcttatcg acttatgttt tatggcttga accatttgcc
     1621 gtagctttgg ttgctttttc cagcagattc gtgttgcgat tattccatat ttctaaacca
     1681 ggtggcttgt tttttgcgat gctttctgcc atgggaacga gcatgcagct gccaatcgct
     1741 caattaccga tcgtctcact ttattttttt atgggtgtcg tttttgccct gattgcggca
     1801 gtgattacaa aattactgga ttcacggcct gagcagacaa ttgaaaaagc aactttaaaa
     1861 gaaagatttc acgaagagcc tttagtgatc attgattctg ttttctacag cgctgcctta
     1921 tttctttccg tttatgtcag tcacggcttg aatctgcata atccttattg gttgaccttg
     1981 tcatgtgcat cgattttgct agcagaaaac ttggatgcga tgaagcatcg acaagtccaa
     2041 tatctgattg gatcgatggg tgggttatgt gtgtcggcat tcttgtcatt tgtgccgttt
     2101 acgcagttgc agaccatttt tctgatcact tttctatatg gaatcgcaca atttctagtt
     2161 gctagaaatt atgcagttgc taatattttt cttaatccga tggcgttgat gctatctaca
     2221 cttattcgtg gagcatactt gatttctttg atcgaatatc gttttttagg gatcgtcata
     2281 ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg ttggtttgca acattattta
     2341 acggttgttc gtcaaaaatt agaatagaag gacaaatata tgtgccaaaa gaggttatga
     2401 caagattttt gtcacaacct ctaattcaag gtgttcaaaa gccagattct cagcattaag
     2461 tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa attttatatt tattctatca
     2521 aaaaattttg tttatgatgg taactgaata aactttcctg agatggtcaa tgctttcctt
     2581 gaaagtctta cagatattct ttcttcatag aaagtttaag ttttatagtt agtgtacaat
     2641 aaaagtatgt ttataccata tggtgtccgg ataatcagtc atttaaacaa tgaggttcaa
     2701 agatatgaaa aggggaaatt tgatgaagaa aataagcaat gaaatccaaa ttctggcaga
     2761 aactttaact cttcccaaag tcgttataga gcaaatgatt gtacaagcaa aaaacatcct
     2821 tgatgaggtg tttgagcaat atagtcatga actaatggat cctaataaag caaaggcgac
     2881 tgcaaagaaa atagacaaag tctatgaaga ggaacctttt gcagcattag ctatttatct
     2941 atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca ttgaagattc caagaactat
     3001 ctatttggca acaatgaatg cttttactag atttattcat gaacatttcc aagtaacagg
     3061 aagctatcgc tttgaccgtg gtttttggat ttggcattac actagtggct tgatctttcg
     3121 gattggtgaa ttggagtacg aaatgacata ttttccgaaa gggtacacgg atctaagatt
     3181 agaaggaaag cagtgccttt ctcttcatat tccttccgat gcagatttat ctcagaataa
     3241 gataagagat agttacgaaa gtgccattcg tttttttgaa ctttattttc ctgattacca
     3301 ttaccaagtg atgtacacag atacttggtt actatcaccc aatcttacta aatggttgaa
     3361 aaaagagtca aaaatctgtc tttttgcagc cgattatcga ttattatcag ttgatgaaca
     3421 ggacgatagt ggagtccctt ggatctttgg acgagtagat gcccaaatac atgattatcc
     3481 agaaagtacg agtttacagc gacaagcaaa agagcaattg cttgcaggcg agcacattgg
     3541 ctctggctta ggtattctta cttcataagt ttagtacttc gaaatagttt aaaagagcgt
     3601 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
     3661 gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
     3721 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt agtgttctat
     3781 cgaacacaca gtacgttcaa taggtaaact agctataaca atagatatta aagttaacgg
     3841 acaagagggc cttttttagg tagctaaaac gtaataatga aaatttattt ccagagatag
     3901 gagtcaatca gctagtctga tcgactgttt taacaagttg actttcattt taacttttga
     3961 taccatttaa aacacaaatc gtaattgttt cgatttgtgt tttttagcat gactacaatc
     4021 aaagaagaaa gtgggtcgtc catatggaaa aaacagatat ctcaagtgca taccgccgct
     4081 tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa gatcatcaaa gatgtgaaac
     4141 ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa aaaaatagcc gaaaaacgtt
     4201 tttcggctat tttcaataat cggtttttgt gtaaggtttc cgaaagaact gctgttgatt
     4261 ttccattttt aacatatgat aaattggtag aatctttctt ttcaaattga taccattccc
     4321 taattccagt tatatagtta actacagaac cacaaagcac gtacgtttat aaaagtatta
     4381 tctgtagtta tttttcaatt ccctttcaac ctcttatttt gaccagtagt attgatcact
     4441 ttacctttta acacctttgt tcgtttttga aacattttta gtcaagaatg gaaacgtaac
     4501 tacataatca aaggttttgc gaaatatttc atgaaatatt tcatgtagaa gcgagcaata
     4561 cggttgcaaa taaccattat cgtttaataa gcagttacga aagctcccaa tatctctaac
     4621 gttcagctca ctccatttct tttcttcaaa aaaatcagtg attcgtatct ctttctattt
     4681 gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata tgcaaaaaaa taaaaaagca
     4741 gttactattg ctagaataac atcaaggaat aataagtttt ggttttcaga taatagtcga
     4801 taagttgtgt attggaattt tagatacagt aataaagtat ttttcatgaa attttgtgag
     4861 aatcactatt acaagtatct ttaataaaat taatggttaa ggaggaactt aaagtagaat
     4921 gtaaatgaat agttgtagtc agtcgattgt gatactagac tcaaagttgc aggaataagt
     4981 ttttcaattt gtgattggaa gataaaaaat gaaaaagaag ggcttttttt tatactttgt
     5041 tgattgtagt gtttattttc cctctgttta gccaagtaac attttctatg actcaaccag
     5101 tttcaagtga ggaacatgat aacctttttc gtgctacaga agagtttaag aaatatcagg
     5161 aaaatattgt tccggagact ccattagcta gtgagccaat tactgatatt gatggaaacc
     5221 aaataggatg gtatatacag tacgaagtta tggtggtaaa taatgactca atagtaaaaa
     5281 atacagtagg cttagagcta acaccgattg aaaaggtgag ttcattgtta acgtatattt
     5341 atactgatga aagtgacgag actgagatca ttttaacgga ttactcaaaa attgctactg
     5401 ataaaaagta tatattgtgg atgtagacac tagtgaaata ctttatgata tagaaattga
     5461 tgaaaattta ctagatgtag cacaagaaat cgatcatcaa aagcaggaag ctattcgata
     5521 tgctggtaga aaagggaaaa gaaaatattt attacgtgaa tgtagcaaaa gttagagaag
     5581 atgaaaatga gtggaaagaa ttcaagagtc gctactcaat taactcgact ccgacattta
     5641 cagtttacag agaaggatcg atagaaaaaa ctgttttttg gactaaagaa agtggaatgt
     5701 ctttagctga agtagaagag tttttggatt atgttagtat gcaacagtaa gggagttctt
     5761 attatgaaaa attttgacac atattatgtt attgtttttt cgatatcttt gctgttaata
     5821 ttacta
//
LOCUS       DARBER010000139         5809 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARBER010000139 DARBER010000000
VERSION     DARBER010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.143"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SCK06_002469"
     CDS             450..1604
                     /locus_tag="SCK06_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG1152416.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SCK06_002470"
     CDS             1598..2020
                     /locus_tag="SCK06_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152417.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SCK06_002471"
     CDS             3143..4075
                     /locus_tag="SCK06_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1152418.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SCK06_002472"
     CDS             4092..4607
                     /locus_tag="SCK06_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1152419.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SCK06_002473"
     CDS             4903..5184
                     /locus_tag="SCK06_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152420.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SCK06_002474"
     CDS             5186..5602
                     /locus_tag="SCK06_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152421.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          962 c          930 g         1689 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtacacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARBER010000140         5791 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARBER010000140 DARBER010000000
VERSION     DARBER010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.144"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1229)
                     /locus_tag="SCK06_002475"
     CDS             complement(99..1229)
                     /locus_tag="SCK06_002475"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG1152422.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="SCK06_002476"
     CDS             complement(1877..3079)
                     /locus_tag="SCK06_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1152423.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="SCK06_002477"
     CDS             complement(3139..3342)
                     /locus_tag="SCK06_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG1152424.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="SCK06_002478"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="SCK06_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="SCK06_002479"
     CDS             4879..5691
                     /locus_tag="SCK06_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152425.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          922 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta g
//
LOCUS       DARBER010000141         5753 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DARBER010000141 DARBER010000000
VERSION     DARBER010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.145"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            146..1042
                     /locus_tag="SCK06_002480"
     CDS             146..1042
                     /locus_tag="SCK06_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1152426.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1192..2133)
                     /locus_tag="SCK06_002481"
     CDS             complement(1192..2133)
                     /locus_tag="SCK06_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1152427.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2259..3380
                     /locus_tag="SCK06_002482"
     CDS             2259..3380
                     /locus_tag="SCK06_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1152428.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3638..5140
                     /gene="eat(A)"
                     /locus_tag="SCK06_002483"
     CDS             3638..5140
                     /gene="eat(A)"
                     /locus_tag="SCK06_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1152429.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5168..5734
                     /locus_tag="SCK06_002484"
     CDS             5168..5734
                     /locus_tag="SCK06_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1152430.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1886 a         1017 c         1212 g         1638 t
ORIGIN      
        1 aattttaata gaagaatgat ttatttcatc atattttcat acaaatgtta aattttcata
       61 caaatatagg aaacgtttta ctggcttttt actatttaaa caggctatca taggaaaaag
      121 tgacagagaa agtgaggcgg aaaagatgaa caaaaatgac gtactgctag ttgcaaaaaa
      181 taaatcactt tattttttgc cttccatccg ctctttagat gtttggtccg gaaaagattt
      241 actacagtcg gaattcatta aatcagctag acaacagttg gcaaattatc agctgatcgt
      301 ttttttagat tatggatttg atatggagat ggctcgacgc gtgcgcccat ttaccaatgc
      361 aaaaattgtg ttattttttt ggaatcactt caaagcagaa cacgaggcga tgctgaagca
      421 ggcacaagat gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga
      481 acttgggctg aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga
      541 accaaagacg gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag
      601 ctataagcag gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg
      661 agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc
      721 gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga
      781 atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt
      841 ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt
      901 tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa
      961 ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct
     1021 ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa
     1081 caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct
     1141 taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc
     1201 gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc
     1261 aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa
     1321 acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg
     1381 atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt
     1441 tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg
     1501 cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt
     1561 tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt
     1621 tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt
     1681 ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc
     1741 tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc
     1801 ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg
     1861 gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac
     1921 gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc
     1981 tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca
     2041 acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc
     2101 cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt
     2161 tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa
     2221 aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg
     2281 attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg
     2341 aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag
     2401 aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat
     2461 tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca
     2521 tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat caaactatcc
     2581 gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag
     2641 aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc
     2701 aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa
     2761 aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag
     2821 atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag
     2881 gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa
     2941 taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta
     3001 ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc
     3061 aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc
     3121 ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa
     3181 aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt
     3241 ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa
     3301 tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt
     3361 tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat
     3421 ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt
     3481 cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt
     3541 tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg
     3601 aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa
     3661 tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt
     3721 tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact
     3781 gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc
     3841 ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat
     3901 tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga
     3961 aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc
     4021 acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga
     4081 tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt
     4141 ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa
     4201 aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg
     4261 tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa
     4321 aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca
     4381 agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc
     4441 tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga
     4501 gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc
     4561 gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt
     4621 attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa
     4681 cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat
     4741 aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga
     4801 caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt
     4861 gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat
     4921 gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact
     4981 atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca
     5041 actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga
     5101 taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca
     5161 acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac
     5221 gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc
     5281 gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc
     5341 tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc
     5401 agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt
     5461 tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt
     5521 cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt
     5581 attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt
     5641 gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat
     5701 ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta ttt
//
LOCUS       DARBER010000142         5642 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DARBER010000142 DARBER010000000
VERSION     DARBER010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.146"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            196..921
                     /locus_tag="SCK06_002485"
     CDS             196..921
                     /locus_tag="SCK06_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152431.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="SCK06_002486"
     CDS             911..1420
                     /locus_tag="SCK06_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152432.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="SCK06_002487"
     CDS             complement(1490..2938)
                     /locus_tag="SCK06_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1152433.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="SCK06_002488"
     CDS             complement(2938..3654)
                     /locus_tag="SCK06_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1152434.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="SCK06_002489"
     CDS             complement(3635..3985)
                     /locus_tag="SCK06_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG1152435.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="SCK06_002490"
     CDS             4129..4902
                     /locus_tag="SCK06_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1152436.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="SCK06_002491"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="SCK06_002491"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1636 a         1111 c          980 g         1915 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accatttgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt aagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 ggcaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 ta
//
LOCUS       DARBER010000143         5615 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DARBER010000143 DARBER010000000
VERSION     DARBER010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.147"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..2009)
                     /locus_tag="SCK06_002492"
     CDS             complement(60..2009)
                     /locus_tag="SCK06_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1152437.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="SCK06_002493"
     CDS             complement(2051..3109)
                     /locus_tag="SCK06_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEG1152438.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="SCK06_002494"
     CDS             complement(3228..3683)
                     /locus_tag="SCK06_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152439.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="SCK06_002495"
     CDS             complement(4267..5070)
                     /locus_tag="SCK06_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1152440.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2077 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa aaaaggaaaa
     5581 ggaggaagct tcagcttccc cttttattta tattt
//
LOCUS       DARBER010000144         5591 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARBER010000144 DARBER010000000
VERSION     DARBER010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.149"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            66..767
                     /locus_tag="SCK06_002496"
     CDS             66..767
                     /locus_tag="SCK06_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG1152441.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="SCK06_002497"
     CDS             764..1885
                     /locus_tag="SCK06_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG1152442.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="SCK06_002498"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="SCK06_002498"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG1152443.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="SCK06_002499"
     CDS             3264..4172
                     /locus_tag="SCK06_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG1152444.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="SCK06_002500"
     CDS             4207..4479
                     /locus_tag="SCK06_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG1152445.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="SCK06_002501"
     CDS             4490..5536
                     /locus_tag="SCK06_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG1152446.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1817 a          971 c         1215 g         1588 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa g
//
LOCUS       DARBER010000145         5535 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DARBER010000145 DARBER010000000
VERSION     DARBER010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.150"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..988)
                     /locus_tag="SCK06_002502"
     CDS             complement(<1..988)
                     /locus_tag="SCK06_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG1152447.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR"
                     /locus_tag="SCK06_002503"
     CDS             1140..1835
                     /gene="vanR"
                     /locus_tag="SCK06_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1152448.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="SCK06_002504"
     CDS             1813..2967
                     /locus_tag="SCK06_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1152449.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="SCK06_002505"
     CDS             3182..4150
                     /locus_tag="SCK06_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1152450.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="SCK06_002506"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="SCK06_002506"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1152451.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..>5535
                     /locus_tag="SCK06_002507"
     CDS             5180..>5535
                     /locus_tag="SCK06_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEG1152452.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1670 a         1078 c         1252 g         1535 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcgg
//
LOCUS       DARBER010000146         5342 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARBER010000146 DARBER010000000
VERSION     DARBER010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.151"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..279)
                     /locus_tag="SCK06_002508"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="SCK06_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="SCK06_002509"
     CDS             complement(545..739)
                     /locus_tag="SCK06_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152453.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="SCK06_002510"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="SCK06_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="SCK06_002511"
     CDS             2091..3131
                     /locus_tag="SCK06_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1152454.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="SCK06_002512"
     CDS             3955..4287
                     /locus_tag="SCK06_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG1152455.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="SCK06_002513"
     CDS             4906..5247
                     /locus_tag="SCK06_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG1152456.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1771 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     5341 tt
//
LOCUS       DARBER010000147         5248 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARBER010000147 DARBER010000000
VERSION     DARBER010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.152"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(406..780)
                     /locus_tag="SCK06_002514"
     CDS             complement(406..780)
                     /locus_tag="SCK06_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1152457.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="SCK06_002515"
     CDS             complement(803..2203)
                     /locus_tag="SCK06_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEG1152458.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="SCK06_002516"
     CDS             complement(2207..2617)
                     /locus_tag="SCK06_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1152459.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="SCK06_002517"
     CDS             complement(2635..3126)
                     /locus_tag="SCK06_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1152460.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="SCK06_002518"
     CDS             complement(3136..3927)
                     /locus_tag="SCK06_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1152461.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="SCK06_002519"
     CDS             complement(3920..4693)
                     /locus_tag="SCK06_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1152462.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5248)
                     /locus_tag="SCK06_002520"
     CDS             complement(4849..>5248)
                     /locus_tag="SCK06_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1152463.1"
                     /translation="NKYMDSDSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKV
                     LYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKD
                     FFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1677 a         1059 c          690 g         1822 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc
     5221 acagctatcc gagtccatat acttattt
//
LOCUS       DARBER010000148         5030 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DARBER010000148 DARBER010000000
VERSION     DARBER010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.153"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(55..276)
                     /locus_tag="SCK06_002521"
     CDS             complement(55..276)
                     /locus_tag="SCK06_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152464.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(273..563)
                     /locus_tag="SCK06_002522"
     CDS             complement(273..563)
                     /locus_tag="SCK06_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152465.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(560..1744)
                     /locus_tag="SCK06_002523"
     CDS             complement(560..1744)
                     /locus_tag="SCK06_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1152466.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1926..3329)
                     /locus_tag="SCK06_002524"
     CDS             complement(1926..3329)
                     /locus_tag="SCK06_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1152467.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3351..4124)
                     /locus_tag="SCK06_002525"
     CDS             complement(3351..4124)
                     /locus_tag="SCK06_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152468.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4134..4511)
                     /locus_tag="SCK06_002526"
     CDS             complement(4134..4511)
                     /locus_tag="SCK06_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1152469.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4532..4846)
                     /locus_tag="SCK06_002527"
     CDS             complement(4532..4846)
                     /locus_tag="SCK06_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1152470.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
BASE COUNT         1462 a         1012 c          875 g         1681 t
ORIGIN      
        1 taaatccttt cataacaaaa tatatacttc ctattttatc atgaaaaata tctgttaagc
       61 accaatgatt cggttaaata agtttaataa cacatctttc acgccagctg cattgaacac
      121 aagtccgacg gcgattagag caataatcaa gaagccaatg agttttgaaa attctcgttt
      181 aaaacctaaa aacagtccaa tgacaacaat cgctaacaat accaatgact gtgcattatc
      241 caagaaccag ttgtacaaat tctgcccaaa attcatgtga ttgtcctcct ttccttaaga
      301 tatttttctc gataatagat atagcttttt acacgcctta ataattgaaa aggggttaaa
      361 tgctctccat agacggttac tcgattggta taattattat aaaatctcac cttgtaaaaa
      421 tcataggtat agatggtatc accagcttgc cgttttccct caataatgag agacgtatat
      481 ttccctgtat tttgtatata cgcttgaaac tcaatgccac actgaaaccc taacttcttt
      541 aattcttttg ttaaaaggct catgtaatca aatcctccat ggctagatgt tgttgttcaa
      601 ttaatttctc atgtttttct gtcagtttgg cttcctgaat catggtggag ataatggttg
      661 taccgttgag aacatctaaa atgcttgcga tttttaatgt aggggcgact tgatgatgta
      721 accagttcaa tgttcgttca aaagaataag gctctggctg tgtggttagg cgtaatgctc
      781 cacgattttt accgataaac cattcccaac gttcattagt tttccaatca ctccgacgtt
      841 tagtgtcgtc tttatctgca aagcggatat agcgattgat aatttcaaag gctgtttttt
      901 ccgcctgttg atgtgccaat aaatctttca tggcttgggt ggctcggtca ttttttaacc
      961 gaatttcaaa ccgattcttt acttctgcgt cttctaaggg aacatcattt ttcacgtatt
     1021 gctcataatc tttctcatat atacagaaat agacttcact ttttaatgag ccaatataaa
     1081 gtgtgtttcc catgtatggt ttttcatcac ggtgaaccaa ttcaccactg cgataatttt
     1141 taaagcttcg aaagacggaa atgcattctt cctgtttaca tttctttgcc aaatctggaa
     1201 tattcaaaat ccctgtttta tcgttgatag ctaaatccaa gcgtttgaaa atacctttat
     1261 gttccatgca atcttgaaag aaatcatacc agctccgatg ttgagccaat agaaagtttt
     1321 caaattgccg acagcctttt cctttcagtt ctaataaaac acctttactt acatcatgag
     1381 agactaggac aacaatatca ccgaaacgat agtgttcggg ataagaataa tagccaaagt
     1441 cttcgtgtag catataatcc atattcaaac gtaaaacctc accgataatt tgagccacat
     1501 cattggtagg gaatcgaatc cgtacatagt cgaataacat ctccaatggg ttatcgggat
     1561 tgaagcgttc caaagcgttc agtaaatctt ctttcacctg ttgagtaggg actgccttgc
     1621 ctgtttcaat atccgatagg taaggtctgg taatatgaga agcaaccgcc agtttatttt
     1681 gagaaacgcc ataagccagt cgcttttctt tcaattcctt aatccaaatt ttttcgtttt
     1741 ccattcgcaa acctccaatc taaaaaagat gtaaactatg acccttgagt ttacaccttt
     1801 catattttcc gcaatccttt gtaccatcaa cattggttga tgagaattgc ctgttttcag
     1861 ttgtatttgt gcccccctgt tagatagagg gggctaaagg attggcgtgg ctaacgccac
     1921 accaatcaaa gcaagtctga acctgtacct ttcacttcgt gcggtacagg ctcagattgc
     1981 tttttttgaa tgacttttcc tatttctcct aagaaatcgt acctttttgg tacaaggggt
     2041 gtataaaact cactaatcac actttttcct gtatccacgt aacctcttcc cttgatgggt
     2101 tttaagaaaa attgtttttg tgtatcgctt ccgaacatca taccaaagcc catttcagac
     2161 atacgaccca aagccacacg gaagttaaag ttgtcccgaa tcccatcact aaaatatttt
     2221 gcgtctggac gttgacaggc aagaattaaa aagaagcctg cttgtcgtcc taacattaca
     2281 atttgtttga gtttatgaat aacggtggta ctttcacgac ctagcatatc catcattgcc
     2341 acatattcat caaaaataag aaaattggca gacaaaccaa gataagcata attttctcct
     2401 gttttatagt tagggtgttg tttcatggtt tcactgcgat caatcatatc ttcgtaaaat
     2461 tgattgatac atgccaacat atcttcttta cggtaataga cattattcat gaccgcccct
     2521 aagtctgcca aatcagcatt tttagggtca agaatataaa gctttgcatt tgtatgcagg
     2581 agagattcaa tcaatgtgag aataaaatag gattttccac taccagtgcc cccagcaatt
     2641 aacatgtgag ggagagaatc gaactcccaa tagaccgatt ccatcaattt gacacgtcca
     2701 tcttgagcta ccacgtcttc aatgccaatt cgattgccaa tagtatcata aagtaggaca
     2761 tactctacat aagaatcttt caacgttttt tccactaatt cacaatacaa tcctgtttct
     2821 agtttgtttt ctaggtgcaa aagctgatct tgatatttcc ctaaggtgat ttctacatga
     2881 atatggatta gtccatcttt cattcgataa tatattttag gaaaatggcg gattcgttgt
     2941 ttggttttct tgctgggtaa atctttaaag aatccctctg attgagtttc ttcggcttca
     3001 taccaatgat tttctaaaat catacgagct aacttttgac ggtgctctaa ctgttttata
     3061 gaatctatac gatagtgata ataaagccag ctgataagtc cgcatacaaa aagagcagtg
     3121 agaatactgc tccaaaagta tggcgtatga agttgataga aaaatgtatc taactgaact
     3181 gcttgccagt tcaccgattg taacgctctc caatgaaaag ggagtaggac gactagaaaa
     3241 actaccagca atgaaattag tacaaaatga tagaccaagt gcttatcgtt ggctttgatt
     3301 cgctctcctc tgtcatgaag caatggcata aaaatcctcc tttccaccta tcaaaataat
     3361 tgtgtattgt agatacgagc ctgtttatca aaagttggtt gagaaatggc ttcatatttc
     3421 tgataagcga ttcgcttttc aaagcgtaaa tttttttctt tcggataata atgacgtcga
     3481 tagagagtgt gtcctaacag ttttttcgct tcttttgtca acggatagag atagcgaaac
     3541 atgcgtccat tgattttctc aatccctttg tattcgcaaa atgcctggga aagccaatgc
     3601 tttttctcta cctgttcaaa gcgagcattt tcctctaata aaaggcgagc acttcgaggg
     3661 tgtatctttt cccatgactg cttatcacga tagacacttg tcttgaaata cccgcagtaa
     3721 aagaagttgg acgcttgata gacatagcca cattttccct caattccatc tgccagtgta
     3781 tataaaaaat gacagtccgt atgttctttc aaccacttaa ttagagctga aagagcttga
     3841 ctgccaaaat aattggtttg attcatttct ggaagaaaac acatctttcc aatttctaaa
     3901 taatctgtgg tttgaaggga actgtcggga aagagtttgc gtatggtttg taggggttgc
     3961 gtaccccagc ctaattccac aacgcctaat aatttttctt ctgaaaaaat ccctagaaag
     4021 tatttacaca atctagggag tatctttgaa taatggtatt gccgaataaa agaaatggct
     4081 tcatccttcg gaatttcttt caaaatcaat ggtgtgtttg tcataagcaa cacctatttt
     4141 tcactttcgg acgcttcttt tttatatttc tgatgaggtt ttgtctgtcc ttttaataca
     4201 agatcgtctg ctttcacgta ccaatcaacg tctgcccctc gaaaagtagc cgtcgctacg
     4261 gtatctgcca caggattgat taattccact tctgcgttgt agtcaaattc tttcaaagga
     4321 acagaagcgg ggatactgac ttgtatcatg cgtccttgtc cttttgattt taagtcatag
     4381 gtacgttctt taatttcttc ggaaaccgta ccatcttcat tctggatacg aacctcacga
     4441 cgcaaggcgg aaaatttcaa tgtgccaaat gtctggtctt tttcaatcac aataccttct
     4501 gctaatctca tattttttca cctttccttt cttatgcttt caccatatcg tctgcgtgca
     4561 taatgtaatt ggtaaatcct cgattgccaa ttttataacc ttctgcggta atatgtgggt
     4621 tgatgagttt gactttttcg tcggattcaa aatgtttctc gccagcttcg gagggaagaa
     4681 tgacaacaat atcatctgct cgttgaatcg ttgagtataa attgtaacta cgagagagaa
     4741 tggttggtct gccatttatt cgacgttgtt caatgttccc ttcaccagca tattctaatt
     4801 gtccaaatgt tttttccata ttgggaatga catatttaag ttccatattt ttacctatcc
     4861 tttctgtttt tatgtttgaa tgaatcttat ttgtgtgatg gttttaaagg ggggagagcg
     4921 accttttgat tcttgatttt ttattttcat aagttcactt cctttcaaaa ttgggtaaaa
     4981 aaatagacgc tccgtactga gcgtccgatt cctactatat ttaaaacaac
//
LOCUS       DARBER010000149         4957 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DARBER010000149 DARBER010000000
VERSION     DARBER010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.154"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..466)
                     /locus_tag="SCK06_002528"
     CDS             complement(26..466)
                     /locus_tag="SCK06_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1152471.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="SCK06_002529"
     CDS             626..1132
                     /locus_tag="SCK06_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1152472.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="SCK06_002530"
     CDS             1300..3213
                     /locus_tag="SCK06_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1152473.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="SCK06_002531"
     CDS             3218..4219
                     /locus_tag="SCK06_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1152474.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="SCK06_002532"
     CDS             complement(4421..4627)
                     /locus_tag="SCK06_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG1152475.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1833 a          759 c          999 g         1366 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
     4921 aactgtgaaa taaaattaca aatgataacc taaaatg
//
LOCUS       DARBER010000150         4878 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARBER010000150 DARBER010000000
VERSION     DARBER010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.155"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(7..1164)
                     /locus_tag="SCK06_002533"
     CDS             complement(7..1164)
                     /locus_tag="SCK06_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152476.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="SCK06_002534"
     CDS             complement(1167..1523)
                     /locus_tag="SCK06_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152477.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SCK06_002535"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SCK06_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG1152478.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="SCK06_002536"
     CDS             complement(2991..3830)
                     /locus_tag="SCK06_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1152479.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..>4878
                     /locus_tag="SCK06_002537"
     CDS             4015..>4878
                     /locus_tag="SCK06_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1152480.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDP"
BASE COUNT         1585 a          970 c          721 g         1602 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcct
//
LOCUS       DARBER010000151         4796 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARBER010000151 DARBER010000000
VERSION     DARBER010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.156"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(182..1144)
                     /locus_tag="SCK06_002538"
     CDS             complement(182..1144)
                     /locus_tag="SCK06_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1152481.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="SCK06_002539"
     CDS             complement(1146..2585)
                     /locus_tag="SCK06_002539"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG1152482.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="SCK06_002540"
     CDS             2770..4716
                     /locus_tag="SCK06_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG1152483.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1533 a          816 c          788 g         1659 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacatt
//
LOCUS       DARBER010000152         4788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DARBER010000152 DARBER010000000
VERSION     DARBER010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.157"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            564..989
                     /locus_tag="SCK06_002541"
     CDS             564..989
                     /locus_tag="SCK06_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152484.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="SCK06_002542"
     CDS             1019..1183
                     /locus_tag="SCK06_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152485.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="SCK06_002543"
     CDS             2141..3118
                     /locus_tag="SCK06_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1152486.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="SCK06_002544"
     CDS             3128..3625
                     /locus_tag="SCK06_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152487.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="SCK06_002545"
     CDS             4001..4282
                     /locus_tag="SCK06_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152488.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="SCK06_002546"
     CDS             4284..4700
                     /locus_tag="SCK06_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152489.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          816 c          785 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtatacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DARBER010000153         4783 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARBER010000153 DARBER010000000
VERSION     DARBER010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.158"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(132..1940)
                     /gene="pepF"
                     /locus_tag="SCK06_002547"
     CDS             complement(132..1940)
                     /gene="pepF"
                     /locus_tag="SCK06_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG1152490.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2116..3114)
                     /locus_tag="SCK06_002548"
     CDS             complement(2116..3114)
                     /locus_tag="SCK06_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG1152491.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3340..3993)
                     /locus_tag="SCK06_002549"
     CDS             complement(3340..3993)
                     /locus_tag="SCK06_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG1152492.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4325..4723)
                     /gene="spxA"
                     /locus_tag="SCK06_002550"
     CDS             complement(4325..4723)
                     /gene="spxA"
                     /locus_tag="SCK06_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1152493.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1411 a         1003 c          751 g         1618 t
ORIGIN      
        1 tacaaaaaag atgttggtga agttcaccaa catcaaaaag tatagagcct taaaaaaata
       61 cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga gaaaatcaaa
      121 aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg ttgttcaaac
      181 atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc ttttttcatt
      241 acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt ctctagtgcc
      301 tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa gccagtcgag
      361 tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc gaatttaatt
      421 tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc ataactatca
      481 cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat gaaatgctcg
      541 aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa gtaatggttg
      601 agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc cgttaggata
      661 ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc gtacacataa
      721 ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg gacaagcgta
      781 aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt gtcatagctt
      841 cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat ccaacgttca
      901 gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag tgcttctaaa
      961 gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa gaccggtgta
     1021 taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa ttccatgtat
     1081 cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta tacactttca
     1141 ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg gactttcgct
     1201 ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt tctaaattgt
     1261 tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc cgtgctttct
     1321 agcaactgac catacacgcc atgagataat tggactattt caccattttc tccttcaatc
     1381 gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga agcatcaaag
     1441 atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg ggctcgatta
     1501 tctactattt gctggatata atggcgatag acttccaatt ttggttcttc tttgaaatat
     1561 tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga aacggcttca
     1621 ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc tgtatttcca
     1681 gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg gcgataaacc
     1741 ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag tgttcctttg
     1801 tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc aaactcttgg
     1861 tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc ttctcgtttt
     1921 ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct atctatcaaa
     1981 atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc attcatgtgt
     2041 ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc gattcctagc
     2101 ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt taaaaaactg
     2161 ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat gttcttttat
     2221 ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt cttcgtacaa
     2281 cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt tttgatagat
     2341 ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt gtatggatac
     2401 tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat cttcactcac
     2461 tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata tttctttaag
     2521 gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg atacagtctc
     2581 tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc agcaatgcac
     2641 tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt gcgattgtcc
     2701 aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat aattttgggt
     2761 tctttcaata aatctctggt gagacagtct agaacattgg atttctattg ctagattcgc
     2821 caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa atactggttc
     2881 tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct cttttgattc
     2941 tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag cacatttttt
     3001 ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt tctttccttt
     3061 ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca gcatttttct
     3121 ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg cgtgaaaaag
     3181 ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata tttttcgttt
     3241 ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa aaaagactgc
     3301 gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat aaaatttcgt
     3361 tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg tttctggtgt
     3421 acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta catccgcttg
     3481 gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga ataaatacaa
     3541 atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt gttcaaaatc
     3601 ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag ataattcttc
     3661 ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt ctgaatcaac
     3721 agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga taaacagttc
     3781 taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc ggaactcctc
     3841 ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt tgtgattgcc
     3901 aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa ttagtacacg
     3961 gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt tgccacaatg
     4021 tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa atctcgctgt
     4081 taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt atttaactat
     4141 aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc cctctctatt
     4201 tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa tactttttgt
     4261 aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt ttcgtattat
     4321 ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt acatcgcgcg
     4381 gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta tcatcaatca
     4441 tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat tcttgtaacg
     4501 gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc gagatgattt
     4561 cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg ttcaatggtt
     4621 ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac gcacgagcct
     4681 ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac cactcctttt
     4741 cgtgataaga atcacttttt tgtaacagaa gatcataata atc
//
LOCUS       DARBER010000154         4777 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARBER010000154 DARBER010000000
VERSION     DARBER010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.159"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="SCK06_002551"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="SCK06_002551"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG1152494.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="SCK06_002552"
     CDS             1085..2005
                     /locus_tag="SCK06_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1152495.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="SCK06_002553"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="SCK06_002553"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG1152496.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..>4777
                     /locus_tag="SCK06_002554"
     CDS             3311..>4777
                     /locus_tag="SCK06_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152497.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTE"
BASE COUNT         1680 a          770 c          924 g         1403 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaa
//
LOCUS       DARBER010000155         4748 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARBER010000155 DARBER010000000
VERSION     DARBER010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.160"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            72..1118
                     /locus_tag="SCK06_002555"
     CDS             72..1118
                     /locus_tag="SCK06_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1152498.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="SCK06_002556"
     CDS             1135..2358
                     /locus_tag="SCK06_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1152499.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="SCK06_002557"
     CDS             2447..3334
                     /locus_tag="SCK06_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG1152500.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="SCK06_002558"
     CDS             3420..3650
                     /locus_tag="SCK06_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG1152501.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="SCK06_002559"
     CDS             complement(3707..4672)
                     /locus_tag="SCK06_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1152502.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1605 a          789 c          966 g         1388 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctat
//
LOCUS       DARBER010000156         4625 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARBER010000156 DARBER010000000
VERSION     DARBER010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.161"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1401
                     /locus_tag="SCK06_002560"
     CDS             <1..1401
                     /locus_tag="SCK06_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1152503.1"
                     /translation="IKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTYDLTISTA
                     TLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLLNITKLWQ
                     DVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILK
                     WGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGW
                     WINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETA
                     KFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASG
                     LLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRK
                     RAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINISYIKQLCY
                     RGRNIISMIKSREHKR"
     gene            1437..2153
                     /locus_tag="SCK06_002561"
     CDS             1437..2153
                     /locus_tag="SCK06_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1152504.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            2172..3755
                     /locus_tag="SCK06_002562"
     CDS             2172..3755
                     /locus_tag="SCK06_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG1152505.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3890..4468
                     /locus_tag="SCK06_002563"
     CDS             3890..4468
                     /locus_tag="SCK06_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152506.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
BASE COUNT         1690 a          518 c          844 g         1573 t
ORIGIN      
        1 attaaaaata tgggaatatt aacaataagt aatttttctt caaaaatttt agtttttttg
       61 ttagtccctt tatatacaag tgtattatcc acaacagaat atggcacata tgatttgaca
      121 atttcaacag ccacattatt gtttccaatt ttaaccttaa acattgtaga tgctgtgatg
      181 cgctttttaa tggataaaga taaaaataaa aaaaatatag catctatagc gattaaagtt
      241 atatttttaa gcgtgatagt ctttggatcc gtaatgtatt tgttaaatat tacaaaatta
      301 tggcaagatg tttatggatt agaaaaatat atttttttgt attatatatc ctatgtatgg
      361 aatcaatttc taatacaatt tgctaaaggg ttagaaaaag ttaaagctat gggcatagca
      421 ggtattatca gcacaattgt aacaataatt gccaatattt tcttcttgct tattctgaaa
      481 tggggattaa ttggtttttt cattgcaaat attttatcac agattatttc agctacttat
      541 ctgtcagtat tagtgagaat atgggaatat atcgattttg aggctaaaga tcctcagcta
      601 aagagagaaa tgctaaatta ctcgattcca ttaattgcaa ctgttgttgg atggtggatt
      661 aatagtacag ctgataggta tgtggtttcc tttatagtag gtgtctcggc gaatggctta
      721 ttgtctgtgg catataaaat acctcaaata attaatactt tgcagggaat atttatacag
      781 gcctggcaaa tttctgcaat taaagaatat ggagataatg agactgcaaa attttatgga
      841 aatacatttg ttatgattaa tttgttaatg tgcataacct gttcaagtct tatcatatta
      901 actagaccgt tttcacatat tctttatgcg aaagactttt atgaagcgtg gaaatatgtt
      961 ccatttttat taatagctaa tgttttaaat tgtgcatcag gtctgttagg acctatctta
     1021 tcagcaaaaa aagattctaa atccatgatg tggtctgcaa tcataggggc attagttaat
     1081 gtgattatga gtgtagtcct tgtttataag ataggggttc aaggtgccac aatagccact
     1141 gttatatgtt catatgtaat atatgttgtt cgtaaacgag ctgtgggaaa agatattttt
     1201 ataaagcatt atcatattgt tgtggaatct tggggattat tgtttgttca ggcaatcata
     1261 gaaatatatt ttaaaaatta tgtaatagaa attataatta tgattatttt aattattata
     1321 aatatcagtt atattaagca gttgtgttat agaggaagaa acattatttc aatgattaag
     1381 agtcgagagc acaaacggta gacgatgtta tttttataaa aagatataat ttattaatgg
     1441 tcgataatta tgaaaggaaa aatattctta aaaaaaagag tataggatgt cgtatgaaaa
     1501 tagtcgctat agtaccaatg aagttaaata acaaaagatt gccacaaaaa aacacgaaag
     1561 cttttaccaa tggaaaacca ttgtgttatt atatactttc aacactttta aaagtaaaac
     1621 agattgataa agtgtatgtc tattgtagta acccagatat taaagaattt ataccagaag
     1681 gtgtaacatt tttaaagaga tctagttcct tagatcagga tacaacgagt attacacagg
     1741 tattagccgg tttcacaaaa gaagtagagg ctgatgtata tgtaatgaca cacacaacag
     1801 caccatttat tagcgaaaaa tctattgaaa aaggactaga tgccgtgctg acaggagaat
     1861 atgattctgc atttgctgca aaaaaattgc aagatttctt atggaaagac ggtgaaccat
     1921 ttaattatga attggacaat attcctagaa cacaggattt gccgccttta tacgaagaaa
     1981 ctagtggatt ttatatttat aatcgcaatg tgatgaccga gttgcataga agaattggga
     2041 aaaaaccttg catagttgaa gtaggtgaga ttgagtcagt tgatattgat gaagccgagg
     2101 attttatgat tgctgatgcg atttataatc atcttatttt acaaggtagt taagacaggg
     2161 ggaaggttag gatgaacaat gtacagattt tagattgcac acttagagat gggggctatt
     2221 gtaacaattg ggaatttgga tttgataata caaaaaaaat tatttctgga cttatagaat
     2281 caggaattga gattatagaa tgtgggtttc tcacaaataa aacagtatac aataaaaatg
     2341 ttacaaaata tacgacctta gaagaattag cacagataat tcctgaaaaa agagaaggaa
     2401 aattatttgt agcaatgatg aactatggcg aatataatat agaggatctt ccagattatg
     2461 atggttcatc tattgatgga attaggattg cgtttcataa aaaaaattgc aatgaagcgt
     2521 tgaaattatg tcgtgagata aagaaaaaag gctacttagt ttttgttcaa gctatggtat
     2581 ctctcaatta tacagatgaa gagtttttag cagtaataaa aaatgtgaat cagtttaaac
     2641 catacgcttt ttatattgtt gatagttttg gaatgatgaa aggaaaagat ttaactcgtc
     2701 ttttttacat gattgagcat aatctagaaa aagatataca gataggtttt cactcacata
     2761 ataacatgca gcttgcatat tctaatgcac aaaaacttat aaccgtacaa acaaatcgaa
     2821 gcctgattat tgattcttcg gtgtacggaa tgggaagagg agcaggtaat cttaacacag
     2881 aactatttgt agaatatttg aatgataatg caggaaaaaa atatcaatta aaaccattat
     2941 tatatataat tgatgaaata ctaaatagtt tctatcagaa gagttattgg ggatattcat
     3001 taccaaacta tatttcagca gttcataatg ctcatcctaa ttatgcaagc tatctgtcat
     3061 ataagaatac tttaacaatt gaagcaatga atgaaatatt cgaaatgatg gatgaagaaa
     3121 aaaaggtatc ttatgataaa caatatatag aagatttata ctacaaatat atggaaactg
     3181 ggaaaataca ggaggaatat atggaagaac tgagaaaatt tctctcagac aaaaaaattc
     3241 tactgattgc accaggaaaa agttctgtag atgaggtaga aagaataaaa gagtttgtta
     3301 ccaaagaaga agtcgtaata attagtgtaa attttgaata ttctcatata gatatagatt
     3361 acgtattttt aagtaattta agacgttttc atgaattacc gaaagaaagc agaactaaat
     3421 gtattgttac atcaaatata caagcatcgg atgtatattt acggacgagt tataaaaagt
     3481 tgttatctga aagggaagtt gtaagagaca atgcaggatt aatggcaatt agatttttag
     3541 ccgatatgaa tgtggatgaa atattcttag ctggatttga tggatattca cataatgaag
     3601 atgagaatta tggtgagcaa tccatggaaa ttataacaaa atttgcatta ttagatgcaa
     3661 tgaacgtgca aatgagagat gaattgtcag aattagctaa aaaagtaaag gtgacatttt
     3721 taacggatcc acgttgggtt cgaattgata agtagtagta ataatgtttt taaaattttg
     3781 ataaagagat agtgagagaa taaaaatggg taaagtctta aataaaaaag aagaaaaata
     3841 tttaagggac gatggggcga gatgaaactc taattttgaa cttttaagaa tcatttctat
     3901 attgtgtatt gttgcttacc atgctaattt gcgtttaaat tataatgcag acggaattat
     3961 agtacatggt tcattaactg ctaaccagat tttcttgaat tatattggta tttggggtca
     4021 gctaggagtt aattgtttta tatttatttc ggcatatttt ttaagtgaga aaagaaacat
     4081 tcactttgaa aatgttttgg gaattgtatt agaatgtatt ttttatagtg tattagttta
     4141 tggaattctt tgtgcatgtg gaatagaaac attttcattg aacagctttg taaaagaaat
     4201 attatcattt gctctaggta gatattggtt tattacatcc tatttagcat tgtatattct
     4261 aagtccgtat ttaaatatca tgcttaacaa tctggaaaaa cttcagttgc aaaaaataag
     4321 tatgttattg tttataggtg tatgctgtta tcgtatgtta tttaaaacat ctttgtttgg
     4381 ggattgtgct gttttaattg cactatatgt catatgttat acactgaaaa aaacgataat
     4441 aatatttttg agagacacag aatcatagga acaattgtat gtataacagt tataccactt
     4501 ctcatatcca taggaatact tttcaaattt ataccaaatg caatggttaa ccagatggtt
     4561 catattggtt ctccattgat ggttatagca agtatatttt tattttatgt atttaaaaat
     4621 ttttc
//
LOCUS       DARBER010000157         4463 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DARBER010000157 DARBER010000000
VERSION     DARBER010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.162"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            311..1042
                     /locus_tag="SCK06_002564"
     CDS             311..1042
                     /locus_tag="SCK06_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1152507.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1187..3214
                     /locus_tag="SCK06_002565"
     CDS             1187..3214
                     /locus_tag="SCK06_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1152508.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3322..4419
                     /locus_tag="SCK06_002566"
     CDS             3322..4419
                     /locus_tag="SCK06_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1152509.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1565 a          717 c          923 g         1258 t
ORIGIN      
        1 atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc aagtcacatt
       61 ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct gtatttctag
      121 attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga taatcattta
      181 taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca taagtatcgt
      241 aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa ataaagaaag
      301 aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt tattcagcat
      361 ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat ataacacaga
      421 aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg atccagagaa
      481 tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa caggaggggc
      541 attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt ctactgcgga
      601 taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac cgacatatgt
      661 ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct ctcaaccaga
      721 acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat ttacgaaagg
      781 gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt ctgaattaac
      841 gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa caggtgtggg
      901 gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg tacagctttc
      961 ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag tttggacatt
     1021 agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa tacgtagtca
     1081 tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat ttgtttggat
     1141 acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga aaaagaggag
     1201 gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca tagggtactc
     1261 tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca aactcacttc
     1321 gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg atttaagtat
     1381 ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca aacaaggact
     1441 agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta tttctatcga
     1501 taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg ggaagttact
     1561 gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata agaagattag
     1621 tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac gagatagagc
     1681 gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta atcccagtca
     1741 agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg gccaacctta
     1801 cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt cttctgatcg
     1861 aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt ttgtcacgcc
     1921 taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag ctcctacatt
     1981 tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc aaagaagtct
     2041 tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc tcattaggca
     2101 gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt tttttcaaga
     2161 aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg atgtaaaagt
     2221 caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga taactatcag
     2281 aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga ctagtatcga
     2341 cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc aaattgatgc
     2401 caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt attttattgc
     2461 tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc ctgaaaaagt
     2521 actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat cgaatgaaat
     2581 aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata aagataaaga
     2641 cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag agaagtataa
     2701 agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga atacagaagg
     2761 catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca aactttatct
     2821 ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta gaccattaag
     2881 tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt ttggaagttg
     2941 gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc ttccagaagc
     3001 tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg ttacgatttt
     3061 cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta cagctcatcg
     3121 tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg aggggaaact
     3181 atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata taacaaaatt
     3241 attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga gacaagattt
     3301 tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc aaaacaacaa
     3361 ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc agtgtcagct
     3421 agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga caagagaaaa
     3481 acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc ggaattgaga
     3541 aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac caccaattca
     3601 aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct gatctttgat
     3661 ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag tgtccaaacc
     3721 gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc tggtgggatc
     3781 acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca aagcttatcg
     3841 attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact gaacgaagtt
     3901 gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag aaatgtcatc
     3961 ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat tgaaacaaaa
     4021 attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg attgcagatt
     4081 gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct aaccccaggt
     4141 gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa attttcgaga
     4201 aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa agattttaca
     4261 atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa aaaagacaat
     4321 aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt atgcatactt
     4381 ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa caaatagtaa
     4441 atttttatta ttgtaggatg gtt
//
LOCUS       DARBER010000158         4461 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARBER010000158 DARBER010000000
VERSION     DARBER010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.163"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            574..1008
                     /locus_tag="SCK06_002567"
     CDS             574..1008
                     /locus_tag="SCK06_002567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152510.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="SCK06_002568"
     CDS             2298..3221
                     /locus_tag="SCK06_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1152511.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="SCK06_002569"
     CDS             3237..3776
                     /locus_tag="SCK06_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1152512.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DARBER010000159         4433 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARBER010000159 DARBER010000000
VERSION     DARBER010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.164"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(543..2048)
                     /locus_tag="SCK06_002570"
     CDS             complement(543..2048)
                     /locus_tag="SCK06_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1152513.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="SCK06_002571"
     CDS             complement(2045..2731)
                     /locus_tag="SCK06_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1152514.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SCK06_002572"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SCK06_002572"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG1152515.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DARBER010000160         4425 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARBER010000160 DARBER010000000
VERSION     DARBER010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.165"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            690..1658
                     /locus_tag="SCK06_002573"
     CDS             690..1658
                     /locus_tag="SCK06_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1152516.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="SCK06_002574"
     CDS             1669..2484
                     /locus_tag="SCK06_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG1152517.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="SCK06_002575"
     CDS             2552..3262
                     /locus_tag="SCK06_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1152518.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="SCK06_002576"
     CDS             3298..>4425
                     /locus_tag="SCK06_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1152519.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DARBER010000161         4396 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARBER010000161 DARBER010000000
VERSION     DARBER010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.166"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..93
                     /locus_tag="SCK06_002577"
     CDS             <1..93
                     /locus_tag="SCK06_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1152520.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="SCK06_002578"
     CDS             complement(151..1371)
                     /locus_tag="SCK06_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG1152521.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="SCK06_002579"
     CDS             complement(1391..2002)
                     /locus_tag="SCK06_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1152522.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="SCK06_002580"
     CDS             complement(2045..2977)
                     /locus_tag="SCK06_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1152523.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="SCK06_002581"
     CDS             complement(3398..4258)
                     /locus_tag="SCK06_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1152524.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DARBER010000162         4374 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARBER010000162 DARBER010000000
VERSION     DARBER010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.167"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..149)
                     /locus_tag="SCK06_002582"
     CDS             complement(<1..149)
                     /locus_tag="SCK06_002582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG1152525.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYS"
     gene            complement(139..1338)
                     /locus_tag="SCK06_002583"
     CDS             complement(139..1338)
                     /locus_tag="SCK06_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1152526.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="SCK06_002584"
     CDS             complement(1468..2844)
                     /locus_tag="SCK06_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1152527.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="SCK06_002585"
     CDS             complement(2844..3500)
                     /locus_tag="SCK06_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1152528.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..>4374)
                     /locus_tag="SCK06_002586"
     CDS             complement(3510..>4374)
                     /locus_tag="SCK06_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1152529.1"
                     /translation="KEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGK
                     IKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMY
                     SGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGG
                     MVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVL
                     LKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLI
                     KKPKEILSKIS"
BASE COUNT         1375 a          801 c          560 g         1638 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacctacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg
     3781 aatataaatt gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt
     3841 tcataaattc tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct
     3901 aacataatag aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa
     3961 gactcttcaa acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc
     4021 tctttcaatt tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt
     4081 ttatttgcaa tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct
     4141 acaaacacca tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta
     4201 ccagaaaaga tccctataat tttaaattta tgacttttta tttttccact tttttcaata
     4261 tctagtaatt caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct
     4321 tcatgaacaa taattgaatt cttatcattt tcttctatat ttttaccttc ttta
//
LOCUS       DARBER010000163         4325 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DARBER010000163 DARBER010000000
VERSION     DARBER010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.168"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SCK06_002587"
     CDS             complement(<1..281)
                     /locus_tag="SCK06_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1152530.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="SCK06_002588"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="SCK06_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="SCK06_002589"
     CDS             complement(2359..2721)
                     /locus_tag="SCK06_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152531.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="SCK06_002590"
     CDS             complement(2725..3063)
                     /locus_tag="SCK06_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152532.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="SCK06_002591"
     CDS             complement(3042..3461)
                     /locus_tag="SCK06_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152533.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="SCK06_002592"
     CDS             complement(3454..3687)
                     /locus_tag="SCK06_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152534.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4325)
                     /locus_tag="SCK06_002593"
     CDS             complement(3684..>4325)
                     /locus_tag="SCK06_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1152535.1"
                     /translation="NPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFIT
                     QYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWW
                     RLELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAK
                     NTKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1521 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggatt
//
LOCUS       DARBER010000164         3923 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARBER010000164 DARBER010000000
VERSION     DARBER010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.169"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(573..1130)
                     /locus_tag="SCK06_002594"
     CDS             complement(573..1130)
                     /locus_tag="SCK06_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152536.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="SCK06_002595"
     CDS             complement(1309..1920)
                     /locus_tag="SCK06_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152537.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="SCK06_002596"
     CDS             complement(2033..2290)
                     /locus_tag="SCK06_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152538.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="SCK06_002597"
     CDS             complement(2887..3165)
                     /locus_tag="SCK06_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152539.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="SCK06_002598"
     CDS             complement(3199..3450)
                     /locus_tag="SCK06_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152540.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DARBER010000165         3876 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARBER010000165 DARBER010000000
VERSION     DARBER010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.170"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..2257
                     /locus_tag="SCK06_002599"
     CDS             158..2257
                     /locus_tag="SCK06_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1152541.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="SCK06_002600"
     CDS             2453..3316
                     /locus_tag="SCK06_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1152542.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="SCK06_002601"
     CDS             3421..>3876
                     /locus_tag="SCK06_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1152543.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DARBER010000166         3843 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DARBER010000166 DARBER010000000
VERSION     DARBER010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.171"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            286..693
                     /locus_tag="SCK06_002602"
     CDS             286..693
                     /locus_tag="SCK06_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152544.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVNLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="SCK06_002603"
     CDS             707..1108
                     /locus_tag="SCK06_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152545.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="SCK06_002604"
     CDS             1110..1481
                     /locus_tag="SCK06_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152546.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1517..1819)
                     /locus_tag="SCK06_002605"
     CDS             complement(1517..1819)
                     /locus_tag="SCK06_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152547.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2068..2268)
                     /locus_tag="SCK06_002606"
     CDS             complement(2068..2268)
                     /locus_tag="SCK06_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1152548.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2573..3805)
                     /locus_tag="SCK06_002607"
     CDS             complement(2573..3805)
                     /locus_tag="SCK06_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG1152549.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         1272 a          647 c          680 g         1244 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aaacttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaatttca agcgagtttg
     1501 tttgttattt ttttatttaa tctttgtatc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata tcaaacaaaa
     1621 agtatacacc cttcttcatt ctagttttcg cagcaggaat tttgaaatag ttcttattag
     1681 aatagtagag attgattaat aagctatctt cgattgctaa aaagaaaact tctgaattcc
     1741 atactttata aaaatctttg ataaatctat tcgaagggtc aaatttaaac cataattgtg
     1801 tttttccttc catcagcata aaagttcacc tcaaaaagag tatacgaacg aatgttcttt
     1861 ttgtgaaggc ggaatttaat atactgaata atagaaaaca agtacttgtg ccaaattatg
     1921 tgccaaaaaa attcgaattc aacaaaatgt aaacaaatag aaaaccttct atattaagat
     1981 ttcctgcaat aacaaacaca acagaagacg tgtaatagtt agtcaggaac gtacaaataa
     2041 cccctgtatc ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa
     2101 actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat
     2161 agatgcatgc gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt
     2221 gattactaaa gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat
     2281 tcgaattaaa cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc
     2341 ttaactctag cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg
     2401 actttttatt atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag
     2461 taccatataa aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta
     2521 gatcctttat tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc
     2581 aatcgccatt tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca
     2641 tcttgtcaga tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg
     2701 ctaattcttc atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta
     2761 gtgctaagtg attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt
     2821 gagaaatagg tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat
     2881 ctgtatcaag cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt
     2941 ttccatcttt aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct
     3001 ttgtacttga aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg
     3061 tcggcatatt tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa
     3121 gatggttttt aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag
     3181 aaaattgttc tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc
     3241 atgctaagac agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg
     3301 cctcaacttg tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag
     3361 ccgcagccac gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta
     3421 gtgctttgcc attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg
     3481 catcgggatc ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg
     3541 cttgatcgat tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa
     3601 attctctttg gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat
     3661 aagcttcttg tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa
     3721 ctgtgtgtcc tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta
     3781 gaagttcatt aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac
     3841 tat
//
LOCUS       DARBER010000167         3833 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARBER010000167 DARBER010000000
VERSION     DARBER010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.172"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="SCK06_002608"
     CDS             complement(<1..1256)
                     /locus_tag="SCK06_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1152550.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="SCK06_002609"
     CDS             complement(1270..2487)
                     /locus_tag="SCK06_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1152551.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..>3833)
                     /locus_tag="SCK06_002610"
     CDS             complement(2477..>3833)
                     /locus_tag="SCK06_002610"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1152552.1"
                     /translation="IATIVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLAS
                     TYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLI
                     SQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYL
                     THPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAV
                     VMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPH
                     GVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFID
                     ASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVD
                     TFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHE
                     Q"
BASE COUNT         1055 a          828 c          650 g         1300 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atc
//
LOCUS       DARBER010000168         3794 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARBER010000168 DARBER010000000
VERSION     DARBER010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.173"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1319
                     /locus_tag="SCK06_002611"
     CDS             <1..1319
                     /locus_tag="SCK06_002611"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1152553.1"
                     /translation="PEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVD
                     LQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGE
                     FYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQEL
                     NTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSAD
                     TTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIR
                     QKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNK
                     LSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHV
                     GNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1309..2526
                     /locus_tag="SCK06_002612"
     CDS             1309..2526
                     /locus_tag="SCK06_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1152554.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2539..>3794
                     /locus_tag="SCK06_002613"
     CDS             2539..>3794
                     /locus_tag="SCK06_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1152555.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1310 a          654 c          791 g         1039 t
ORIGIN      
        1 caccagagta tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg ttccaattga
       61 acgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt aatgggttat
      121 ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa cgaaatgtga
      181 cgattaccga agtcatcaag aaattgaatg atgtagatgt tttagaacac gatggggacg
      241 tgatcgggga tgcgtatgag tttctgatca gtcaatttgc ttcagaagca gggaagaaag
      301 ccggagaatt ttacacacct catatggttt ctgatatgat ggcgcaaatc gtaacgctcg
      361 atcaaaagga acgacgattc tttagtgttt ttgatccaac gatgggttca ggttcattga
      421 tgttaaacgt acgtaactac ttaacacacc cagataacgt taaatatcat gggcaagaac
      481 taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggt gtcgacgccg
      541 aagaaatgaa tctccgcaat ggggatacgt tgaataagga ctggccaaca gatgaacctt
      601 acacatttga tgcagtagta atgaacccac cttattcggc taattggtca gcagatacga
      661 ctttcttaga tgattctcgg tttaatcgtt atgggaaatt agcgccaaaa tccaaagcag
      721 attttgcgtt tcttcttcat gggttctatc atttgaagga aacaggtacc atggccatcg
      781 tcttaccaca cggagtttta ttccgtggcg ctgcagaagg ggtcatccgt caaaaattac
      841 tagaagatgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc tttgggacat
      901 cgattccaac gacggtgatt gtgttgaaga agaatcgtca aactcgagat gtattattca
      961 ttgatgctag tcgtgaattt gttaaaggga aaaatcaaaa taaactatcg gaagaaaaca
     1021 ttcaaaaaat cctcgaaacc tacgctgaaa gaaaagatgt cgagaaatac gctcatttgg
     1081 caacctttga tgaaatcaaa gaaaatgact ataacttaaa tatcccacgc tatgtagata
     1141 ctttcgaaga agaagaacca attgatatgg ttcatgtcgg aaatgacatc aagaagatac
     1201 gacaagaaca gcaagtgcta gaaaaaaaac ttttagaagc tatctcttcc ctgcaaacaa
     1261 cacctgaaaa tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat gagcaatgat
     1321 acacaaccag aaatccgttt cccaggattt acagatgatt gggaacagag gaagttgggg
     1381 gacttcgttg tagattatgt tgaaaagaca agtgtacaaa atcaatttcc tatgctaact
     1441 tcatctcaac aaaaagggat agttttgcaa gaagactatt ttgcaaatcg gcaagtaact
     1501 acagaaaata atattggtta ctttgtacta cctagaggat atttcacgtt ccgtagtcga
     1561 agtgataatg atgtgtttgt gtttaaccgt aatgacatta ttgatagagg gattataagc
     1621 tatttttatc ctgtatttac tttaaaatct gctgattccg acttcttctt aagacgtatt
     1681 aacaatggaa tccaacgaca attatctatt caagcagagg gaacaggtca acatgttctg
     1741 tctctaaaaa agtttaaaaa tatagtagca atgtttccta gtgaggaaga acaacaaaaa
     1801 atcggcacct tcttcaaaca acttgacgac acgatcgctc ttcatcagcg taagttagat
     1861 ttgctgaagg aaacgaaaaa aggattctta caaaaaatgt tccctgaaaa cggagcaaaa
     1921 gtgccagaaa tccggtttcc tggttttacg gaagattggg aagagcgtaa gttgggagaa
     1981 ttaggtaaaa ctcaatcggg aattggattt cctgatgccg agcaaggtgg ttctgaaggt
     2041 actccatttt ttaaagtttc cgacatgaat aacattggta atgagtatga aatgagaaat
     2101 gcaaatcatt atgtatctaa tgagcaaatt gagcgtaaaa aatggaagcc aataaaagat
     2161 gtgccagcga ttatatttgc taaagttggc gcagcaataa tgcttaatag aaaacgtttg
     2221 gtcacaagcc ctttcttaat agataacaat accatggctt atttatttaa taatacttgg
     2281 gatatctatt ttggtaaaat attgtttgaa actataaatt tgccaagata ctcccaagta
     2341 ggggcattgc cttcttataa cagttctgat attgagaata tcagtgttaa ggttcctgta
     2401 aaggatgaac aacaaaaaat cggtactttc ttcaaacagc tagatgatac gatcgctctt
     2461 catcaacgta agttagattt actaaaagaa acgaaaaaaa gcttcttaca aaagatgttt
     2521 gtctaggagg gaagaagaat gaaaattagt caccgtgagg aagcagaagt cgaagaacag
     2581 ttgattcgtg tacttggaga agggcataat cagtggacgt atcgtccgga tctaaaatca
     2641 gaagaagacc tttgggtcaa tttacgccaa aaaattatct caaataatca agcagaactg
     2701 aatgattttc ctttgaccga taaagaattt gaaacgatca aaacggaatt actgctgcga
     2761 acgaaaaccc catttgatgc tgcgaaatgg ttaaaaggtg aaaatgggat ggctcggatc
     2821 acgattgaac gggaagattc gcaattgggt tctgtctcac ttatccttta ttccaatcaa
     2881 gacattggtg gaggaatctc tacttatgaa gtggtccatc aaattgcaaa acgtggaagt
     2941 aacatagaag cacgggatcg acgttttgac gtcaccttat taatcaatgg attaccgatt
     3001 gtccaaatcg agctgaaaca agtaactgca aaagatggtg tttatcaggc atttaaccaa
     3061 atcaaaaaat atgcagaaga aggtatgttt cggaacaata tcttttcgac cttacagttg
     3121 tttgtggttt ccaatgaaca aaccactcgg tattttgcaa atgccttacc gaaagacttg
     3181 caccctaagt ttttatttag ttggcgaacg aaagacaatg aaaaagtaga aaacttatat
     3241 gagttttgta aacaagtgtt aaatatcccg gatgctcatc gtttgattgc ggattataca
     3301 atcgtcagtg aagatcaaga caataaaacc ctcatggtgt tacatcctta tcaagtacat
     3361 gcgattcaag ccttgtttat tgccgcaaat aaacatcaat cagggtatgt gtggcatgcg
     3421 actggttcag gaaagacgtt gactagtttt gtatctacta agcttttagc acgaaaatcg
     3481 gggatcgatc gaacgatcat gttagtggac cgcaaagatt tagataatca aaccaccaca
     3541 gagtttacaa aatttgcttc ggaatttaat actgggattt cttccgggaa cgccaaagcg
     3601 aatagcttga tcgttggaac gggaagtgcc aaagaattaa gtgaaaccct gttggcagat
     3661 gccaatgcaa atgttgtaat cattacaacc cgtcaaaaac tagatgcggc gttgaaatat
     3721 gcaaagaaac aagaagagaa aaaaggaacc aatcgcttcc aaaaattaat gggccaacat
     3781 atcgtgtttg tagt
//
LOCUS       DARBER010000169         3778 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARBER010000169 DARBER010000000
VERSION     DARBER010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.174"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(697..990)
                     /locus_tag="SCK06_002614"
     CDS             complement(697..990)
                     /locus_tag="SCK06_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG1152556.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1393..2571)
                     /locus_tag="SCK06_002615"
     CDS             complement(1393..2571)
                     /locus_tag="SCK06_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1152557.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2675..2989)
                     /locus_tag="SCK06_002616"
     CDS             complement(2675..2989)
                     /locus_tag="SCK06_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152558.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3096..3314
                     /locus_tag="SCK06_002617"
     CDS             3096..3314
                     /locus_tag="SCK06_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152559.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(3309..3704)
                     /locus_tag="SCK06_002618"
                     /pseudo
     CDS             complement(3309..3704)
                     /locus_tag="SCK06_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1208 a          721 c          553 g         1296 t
ORIGIN      
        1 attttatttt taaataaaaa atactctttt tttacgatta ttgaaaaaaa aagataaaac
       61 ttgattaatt gaagtattgc ttaaatttgg aagctatgag ggaagaataa aagagttagg
      121 acgggattat ctactatttt tctttgtttt aaatatttgt tatctttgaa cataaaagag
      181 aaatgtgtcg actgcagtgt tgtgtccctc atcacgaata aacggtgttc aattagctga
      241 tcttgtattc acttaaaaaa tggtaaagta cgagttgcta ttccccattt tcaattttct
      301 aacgctcagg acaaaatact gttttcacaa cctcattgga ataaaagcat atatattgtt
      361 tcaagaacga aatcttgtta tctttttaaa gggcatctgt ccaaaatcta attttaggag
      421 gtacgttttg gtatttattc gtacaaataa aagagaatta tctttattca tgtaacgcat
      481 ccaattgatt ttttcatgta cgctcctgga acgattttac tgacgattgc ttatttaact
      541 gcgaatcgct cattagcttt tatcatagct atccatattt tgaataacgt tcttggcttt
      601 gtattataga tacagatgac aaaaaaagtt caagtatgcg gtgtagaacc aatatcgcat
      661 acttgaactt ttttcaatga gtataaatta aaacttctaa ttttcaattc ccatcatctg
      721 atatgcccga ccgtctgaac ttcttttgaa aaagccaaat gtgattagat aacgtctttg
      781 ttcgacataa tcgccactag aaaatacgtt cagcagtatc gaattaacct ctgtttctgt
      841 ataagtcact ccatggtcaa agcgtttcaa gacttctttc aataggatgt atttcttttt
      901 tgttgaactt ggtacttgtt ttacttttcc atcattcatg aatgtgtcta aaactttcat
      961 cttttcttgc tccgtataag tataaaccat cttctttctc cttttgattc taagaatact
     1021 ccatttatgt tagaatattc tggatttttt attaaagcgg ttactcttaa gaaagatatt
     1081 atcatagtgc tctttttttt actactactt ataggtagat ttattctaat ccattattct
     1141 cccctaccaa taattaaaga aaaaactgga tcagggtgtt caaattacat gctaagaaaa
     1201 aagaaccctt gaataccaag ggttctttct cagtctgata acaatgatta acgacggatt
     1261 tctttgatac ttgtgaaaag tcgttaaagt attgatataa aaggattttt attacctctg
     1321 actacatatt gactaatttg ttaaaaaatc gtttttcaag aatcctgtaa taccagtgct
     1381 ttcaccgttt ttttaactct ctaaagaact aaaagcaatc agaggtttta ccttttcatt
     1441 tgacgttaag tggcgataaa ttttcgaagt tgtttggtca gtatggccta agcgatcttt
     1501 caagacttcc cagctacagt gttccgctac taagcttgcc attgtatgac gagctaaatg
     1561 agtatgaaat ggcttattaa ttcctgagcg attcacgcac cgtttgacgt gatcatttac
     1621 atactctcga cgataaggaa caccacgttt actagaatca agatacacaa acaaatatgt
     1681 ttcttctata ataaacctgt aatgttgcct catctcaccc aaagccttaa tcaactgttg
     1741 tagcttttcc ataactagag gagttactag aactcgtcta gcagaactaa ctgtcttagt
     1801 agtttcaatt cgaaaatcct tactcttacc tttaactgcc gtttgcttat agaacagaat
     1861 ttcatttctt ttgaaatcta tatctccagg ctgtagagca agcgcctcgc tgactcggca
     1921 gccagtgtaa aatagaacat ggaaaagtac agacatcggt aattcctcat gaacaatgcc
     1981 ataattcaaa aaagttctca attcatcgac agtaagaaac ttgtcctcaa tattatttat
     2041 ggcttctttt aaatcagtta ccgttaactg atattttggc attctcacag acttcattgg
     2101 attttcactt atcaaattct ttctttgaca atatttaaaa atcatattta gaacttgaat
     2161 aagtgattgt aaactatttt tggcatagcc aacatttgtt ccattaacgc cacattcctt
     2221 taaggtaaaa agcaattctt gaaggcactc tggtgtcaaa tcagacaact ttttatcacc
     2281 aatccatcga acaataattt ttaattgttc ctctcgctta tcataggtta tttccttttg
     2341 ttcatttaac gaacggtaat attggaacca ttgctcacct acaacactaa acttgtcatt
     2401 gcttgcaaca atttttttct taagcctttg ttgtttctcc atttccagat aagcgagttg
     2461 cgcctcacgt tttgaattaa agccacgtct tgttgttcga tatgcttgaa agtaccatgt
     2521 gatcctacca gtagaactga tttgcttctt gaatcgatct gttttttcca tttattgatt
     2581 cctcctattt tgaaggaacc caacaagcag aaaccctttg gaaatctttc tatctatgtc
     2641 atcattgtac cgcatcttag aaagaaaatg taacttataa agatcgaagt agtcgcaatt
     2701 tttctcgata gtcattcgaa tcactctttc tcatccattc taaaacatca tcacgaaaat
     2761 agtgtcctcg aatcccgtac caacagggag gaaactcacc aagttttact aaacggttca
     2821 gtgtactatc acttatctga aatagtcgct ttaaatcagt atttgttaat accagaggta
     2881 aatgactgac tgcaagcaat ttttccgtgt aatcttccag ttttttttgt aaaattgaat
     2941 agaactctat ttccagagaa ttaacatttg ttttaacttc caactccatc acccttctct
     3001 ctcaaaatca aaacgaccac cacggtaccc cttaaaattg taaaaaaaca ctccagtact
     3061 ccatcaaaac ggactagaaa agaggaattt ttaatatgaa acaaggaatt gcagacataa
     3121 aaataattaa agaaattctt gaaaaatcta cggctaatgc aatagcattc ggaacaggaa
     3181 tcaacttgag tacagtaaaa aaacttaaaa gtggagagag agctgaagaa aaattaaatc
     3241 ttgccgatgc tattaaaatc acagaatttg gcatgaaaaa tatgccaacc aaaatcgaaa
     3301 tatggaaatc ctagctcttc caaagccatt tgtcgttggt accaaacact gccgagactt
     3361 ttcccaataa tatcgccaat ttcacgatca ttaagaccga tgaagaagga cataaaaatg
     3421 atttcttgtc tttgactagg taatgctgca atcgcatttg ccaactcctc attggaaata
     3481 aagattttca taccaaggac aataaaagta ccactactat tcaaatttgg ttccaaataa
     3541 tagaactctg tacctaaccc tttttgttcc aatatttcca ttgatatttg ccgttggcga
     3601 aatctagcgt acttcttttg aatatttaac gcctcattgc agactacttt cttacaaaaa
     3661 ctgtcaaacg tatgttgaat gtgggtttcc tctgagtcgc ccataatatt ctccccctca
     3721 aaaaattcat tcaactcgtt ttacttctat ccctttccat tattactaca atctagga
//
LOCUS       DARBER010000170         3626 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DARBER010000170 DARBER010000000
VERSION     DARBER010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.175"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..717)
                     /locus_tag="SCK06_002619"
     CDS             complement(133..717)
                     /locus_tag="SCK06_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1152560.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="SCK06_002620"
     CDS             complement(928..1131)
                     /locus_tag="SCK06_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152561.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="SCK06_002621"
     CDS             complement(1141..1563)
                     /locus_tag="SCK06_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152562.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="SCK06_002622"
     CDS             1801..2415
                     /locus_tag="SCK06_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1152563.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="SCK06_002623"
     CDS             complement(2580..3404)
                     /locus_tag="SCK06_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152564.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="SCK06_002624"
     CDS             complement(3428..3583)
                     /locus_tag="SCK06_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152565.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT         1200 a          635 c          517 g         1274 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaa
//
LOCUS       DARBER010000171         3624 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARBER010000171 DARBER010000000
VERSION     DARBER010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.176"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            205..1971
                     /locus_tag="SCK06_002625"
     CDS             205..1971
                     /locus_tag="SCK06_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG1152566.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1986..2423
                     /locus_tag="SCK06_002626"
     CDS             1986..2423
                     /locus_tag="SCK06_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1152567.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2436..3587
                     /locus_tag="SCK06_002627"
     CDS             2436..3587
                     /locus_tag="SCK06_002627"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG1152568.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1220 a          639 c          776 g          989 t
ORIGIN      
        1 tgctaacaaa agttattgtt tgcatattat aaaaaaatta aaataacagt tttatttaaa
       61 attgaagttt tctgataccg aaatcttttt attttgctga catactcaaa cctttggcac
      121 aaagtttcgt gccaaagtac gactatttct ctttttagta agcggataca attagagtat
      181 caaaaggaga gagggagaga caaaatggaa cggacgattc ccaaaaacaa catttcgcta
      241 aaagcgaggg tacaaaaatt aggcagtact ttatcaagca tggtcatgcc aaatattgga
      301 gcactgattg cctggggtgt attaacggct ctatttatcc cagatggtta tttaccaaat
      361 gaggctttgg ctaccatggt aagcccaatg ctaacttatc tgattccttt attgatcggt
      421 tatactggcg gaaaagtaat tgctggtgac cgcggatcag ttgttggggc aattgcaacg
      481 atgggtgtga ttgtcggaac agatattcca atgatgctag gcgcgatgat catgggacct
      541 cttggtggat atgccatcaa gaaatttgat cagctattcc aaaaacggat caaatccggt
      601 tttgaaatgc tagtcaataa tttttcagca ggtcttatcg gttttggatt agcgttatta
      661 ggtttttctg cgattggtcc agttgtcgat gcattgactc aagcaatggc aaaaggtgta
      721 gagatcattt tatctgccca tttgattcca ttgacaagta tttttattga accagcaaaa
      781 atcttatttt tgaataatgc aatcaatcat gggattttga caccattggg aactgaacaa
      841 gtagcagagg ctggaaaatc tattctattc ttgcttgaag caaatcctgg tccaggactt
      901 ggcgtattac ttgcttttac tttgtttggc aaaggagcag caaaatcttc agcaccagga
      961 gccatgatca ttcacttttt aggcgggatc catgaaatct attttcctta cgttatgatg
     1021 aaaccgttat tattcttatc agttataggc ggaggaatgt caggaagttt cattttccaa
     1081 ttgatggatg ctggactaag agcgccagct tctccagggt cgatcattgc gattctagcg
     1141 atgacaccgt tcaattctta ccttcctgtt attttgggcg tgattgttgc cgcagctgtt
     1201 tcgtttggta tttctgcagt aattttgaaa gcggatgcga aagatacaaa tgaagacttc
     1261 gaaaaatctg ttcaagagac aaaggcagct aagcaagctt ccaaaggtgc ccaaggtatt
     1321 cctcaaggag ctacattctc tggcgtacaa aaaatcattt ttgcttgtga tgcaggaatg
     1381 ggatcaagtg cgatgggcgc ttcgatcttg agaaaaaaag tacaggaagc agacttgcct
     1441 caaacggtga ccaatcaagc aatcagccaa ctgacagatg actcgaatac attgatcgtt
     1501 actcaggtcg aactacaaga aagagcaaaa caaaaagcac cgagcgcgcg ttttgtctca
     1561 gtagaaaact ttttgaattc acctcgatat gatgaaatca ttcaagaatt aagcagcaag
     1621 cagaaaacat cagtttctga aattgagaat gagccagcag aagagtttct ttcagcaaac
     1681 atcaaacaaa tcaaagagat tctacttgtc cacgatgatc gttccggatc agcaacaatg
     1741 gggatgaccg tgctcaaaga tatattagac aagaaccatt taactatccc gttaagaaaa
     1801 gtcaatatcc aagatttaaa agatgaacct gattcactga tcgtagccaa agaaagttta
     1861 gcggaaaaag tacagatggc tgcacctcaa tccattcatt tatcagtcaa cagcatggtc
     1921 aatccgcaaa aatacgaatc gattgcacaa tatttgaaac agacagctta agggaggaaa
     1981 acagcatgca aagtttgcca ttcgacatga tcgagctcaa tcgatctttt aaaaccaaag
     2041 aagaagcgat ccgctattgc ggtcagatgc ttgtagaagc agggtgcgtt gatgaggaat
     2101 atatcgatgc catggtagat agagacaaaa tgttatctgt ttatatgggg aattttatcg
     2161 cgattcctca tggaacagat gaagcaaaaa aatatgtgaa aaaatccggc atctgtgtcg
     2221 ttcaagtacc agatggtgta gattttggta ccgaagaaga agaaaagatt gcgactgttt
     2281 tgttcggtat tgcaggagtc ggagatgagc atttgcaact tgtacagcaa atcgcacttt
     2341 attgcagtga tatggacaat gttgtccagc tagcagacgc gctcagcaaa gaagaagtaa
     2401 cggaaaatct agcaattgct taaggagaga gaaaaatgag agcagtacat tttggtgcag
     2461 gaaatatcgg tcgaggattt attggagagg ttttggcagc taatggattt gagattactt
     2521 tcgtagatgt gaatcacacg atcattgatg ctttaaatga aagaaaagaa tacgaaatcg
     2581 aattagcaga tgaatcaaaa gaaaaaatca ctgtaaaaaa tgtcaaagga atcaacaatc
     2641 aaacaaatcc agaaaaagta atccaagaaa tcgctgaagc agatttagtt actaccgcca
     2701 ttggcccgaa tatcttacct tttattgctg agttgattgc aaaaggtatc caagaaagag
     2761 aaaaggaagg aagcacaaca tctttagata tcattgcctg tgaaaatatg attggcggtt
     2821 cagcatttct agaaaaagaa gtgtataaat atcttccgga aacatcattt actgacaaat
     2881 atatcggctt tccaaatgca gcagtcgatc gaatcgtccc attacaacac catgaagatc
     2941 ctttattcgt ccaagtggaa cctttcaaag aatgggtcat tgacgatagc caaagaaaaa
     3001 ataaaaaaat ccaattaaaa ggggttttat atgtagatga tctagaacca tatatcgaac
     3061 gcaaactatt tagtgtcaat actggtcatg cgactgtcgc ttatacaggt gccttgttag
     3121 gttaccagac cattgatgaa gcaatgcaag atgcgttagt tgtggcacaa ttaaaatcag
     3181 ttttgcagga aacaggaagt ttgctgattg ccaaatgggg gtttgatgca gagcagcatc
     3241 atgcgtatat tgaaaaaatc attcatcgct tccaaaacaa aaatatctct gatgcgatca
     3301 cacgtgtcgc tcggacacca ttacgtaagt taggttatca agaaagattt acacgtccag
     3361 tgagagaact acaagaacat aacttgactt gcccacattt gacggcaaca atggggatta
     3421 tttttaatta ctatgatcct gaagatgaac aaagccgtca attgcatgaa atgaagatcc
     3481 atgaaaactt ggaacaattg attcaagaag tcacaggaat caatgatccg aaaacaatcg
     3541 ggaatatcaa gcaaaacgtg aaccgttatg ccaagcaagt cgcttaaaat aggatacgaa
     3601 aaaggggaat gtgacgaaat tttt
//
LOCUS       DARBER010000172         3557 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DARBER010000172 DARBER010000000
VERSION     DARBER010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.177"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            36..1229
                     /locus_tag="SCK06_002628"
     CDS             36..1229
                     /locus_tag="SCK06_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1152569.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            1222..2949
                     /locus_tag="SCK06_002629"
     CDS             1222..2949
                     /locus_tag="SCK06_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG1152570.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            3343..3540
                     /locus_tag="SCK06_002630"
     CDS             3343..3540
                     /locus_tag="SCK06_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG1152571.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT         1212 a          583 c          794 g          968 t
ORIGIN      
        1 agaaaaatga atttacataa aaaaggagaa tttgtttggt aaaagtagaa gtaaaacatt
       61 taacaaagat atttggtaaa aagacacaag ccgctttaga tatgatgaat gatcaccagc
      121 cgaaaacgga gattttaaag aaaaccggtg caacggttgg tgtgtatgac gtgaattttg
      181 acgtaaaaga aggagaaatc ttcgtgatca tggggctttc cgggagtggg aaatccacat
      241 tgatccgtct attgaatcgt ctgattgagc caacttcggg cagtatctat attgatggag
      301 aagatgtatc gaagttaagc aaagaagaat tgcgagaagt ccgtcgtcat aaaatcaata
      361 tggttttcca aaacttcggt ctgtttccac atcgaacgat cttggaaaat acagaatacg
      421 ggttagaagt ccgtggtgta cctaaagaag aacgacaaga aaaagcagaa aaagcattag
      481 aaaattctag tttgctttca tttaaagacc aatatcctag tcagttatct ggtgggatgc
      541 aacaacgggt tggcctagcc agagctttag caaatgatcc tgagatcttg ctgatggacg
      601 aagcgttctc ggctcttgat ccgctgatcc ggcgagaaat gcaagacgaa ttactggacc
      661 tgcaagccaa tgttcaaaaa acgattattt tcattaccca tgatttgaat gaagctttac
      721 ggattgggga cagaatcgcc ttgatgaaag acggcgaaat catgcagatc ggaacaggtg
      781 aagaaatatt gaccaatcca gcaaatgatt atgtacgtga atttgtggaa gaagtcgatc
      841 gttccaaagt gcttacggct caaaacatca tggtacctgc attgacgacg aatatcgaat
      901 ctgatggacc aaacgttgca ttgacaagga tgcgtaatga agaagtcagc atgctgatgg
      961 ctgttgacag aaagagacac ctgaaaggga tcatcactgc agatcaagcg ttagaagcta
     1021 ggaagcaaaa acgtccattg atcgacttcc tagatgaaaa tgtgacggtg attgggaaag
     1081 acatggtggt aagtgatatt ttcaatatta tttatgactc accgactcca cttgcagttg
     1141 tagaggatgg aaaattaaaa ggtgtcgtca tccgaggtag tgtgattgaa gcattagcag
     1201 aaacaagcga ggtgagtgaa catgaataat ctattgaact atcaattacc agtggcaaac
     1261 tgggtagaaa atatcacaga atggtttacg acaacttttt ctggcctatt ttcattttta
     1321 caaacgattg gacaggcagt gatgtcgggg attacaaatt tactgttagt gattccagct
     1381 cctttgttta tcttgttatt gacggtagca gctttcttta tttctaagaa acgtccagga
     1441 cttacgttgt tcacattaat cggtctatgg tttatttata accaaggtct atggaatgat
     1501 ttgatgaata ctgtcacact tgttctttta tctagtgtga tctcgatcat cattggggta
     1561 ccattaggga tcttgatggc aaaaagcagt aaagcccaaa gtattatcaa accgatcctt
     1621 gactttatgc agacaatgcc aggattcgtt tatttgattc cagcagttgc atttttcggt
     1681 atcgggatgg ttccaggggt atttgcctca gtcatctttg ctctgccacc aaccgttcgt
     1741 tttacgaatt tagggattcg acaagttcct aaagaattag tagaagcttc tgattcattc
     1801 gggagcacaa gtcgtcaaaa actattcaaa ctagaattgc ctttagctaa aagtacgatc
     1861 atggcaggga tcaatcaaac aacaatgctg tcactttcaa tggtcgttat tgcctctatg
     1921 atcggtgcac ccggcttagg acgcggcgta ttatcagcat tgcaaagagc acaagtcggg
     1981 aatggatttg tcaacggggt tgcattagtt atcttagcaa tcatcattga ccgttttaca
     2041 cagcatttga accagccaaa caataaaaaa gctgcaggag caacaacaca gaaaagtaaa
     2101 aaacgtcaag gactgatcat tggtgctgta gtagtagtga tcttaggtgc gattggtata
     2161 gggagttttt catctgccaa agaaacaaaa cgtatcaact tatcttatgt tgaatgggat
     2221 acagaagttg cttcaacaaa tgtagtcggt gaagtattga aacaaatggg ctatgatgta
     2281 acgatgacgc cattagataa ttcaatcatg tggaaatctg tttcaaatgg cgagtcagat
     2341 gccatggttt cagcttggct acctaaaact catggttcac aatatgcaca atataaagat
     2401 caagtcgaag acctaggtgc caatttgaca ggagcaaaag ttggtttagc agtgccagcc
     2461 tacatggatg taaattcgat cgatgagtta accgatcagg caggtaagaa aatcatagga
     2521 atcgagccag gagctggtgt agttacggct gctgaaaaca cgatccaaaa atatgacaac
     2581 ttgaaagact ggaaagtaga gacatcttct tcaggagcaa tgaccgttgc actaggtcaa
     2641 gcgatcaaaa aacacgaacc aattgtagtt acaggatgga cacctcattg gatgtttgcc
     2701 aaatatgatc tgaaatactt agaagatcct gaaaacggaa tgggaagcga agagcagatc
     2761 catacgatgg ttcgaaaagg attgaaagaa gaccagccag aagcttacaa agtattagac
     2821 aatttccatt ggtcagaaaa agatatggaa aaagttatgt tggaaatcaa caatggaaaa
     2881 gatccacagc aagcagcaaa agactggatc aaagagaacc aagaattagt cgaaagctgg
     2941 aaaaaataaa aagacagaca cgaaataaat ggagcttcca aaattggatc aaatatccaa
     3001 atttggaagc tccatgttcg tctctatctt ttttgtctat aaaaaaagag aatgcgtagc
     3061 tcatcttcga aacgttcaaa gacgccgaca aactattatg aatttatggg tgcgagtcaa
     3121 agagatcgat cgtaggtatg aaagactaat gtgattaaag tttattatgt acgaagtgca
     3181 ttctcaaaaa atattaaaga atatacatag tttatatact taaaagtgat taaattctta
     3241 ttcaggaatt attttgctac gtttcaagaa ggaattgttt tttgaaattt aatgaatgtt
     3301 agaataatag tctaaaaaca ttaatttagg ggtgattaga ttatgaaaca tttaaaaatt
     3361 ttgtctatta aagagacaca acttatctat gggggtacca ctcatagtgg aaaatattat
     3421 ggaaatggag tgtattgcac taaaaataaa tgtacggtcg attgggccaa ggcaactact
     3481 tgtattgcag gaatgtctat aggtggtttt ttaggtggag caattccagg gaagtgctaa
     3541 aatgaaaaaa aatgcta
//
LOCUS       DARBER010000173         3464 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DARBER010000173 DARBER010000000
VERSION     DARBER010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.178"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(681..1295)
                     /locus_tag="SCK06_002631"
     CDS             complement(681..1295)
                     /locus_tag="SCK06_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1152572.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1901..2278)
                     /locus_tag="SCK06_002632"
     CDS             complement(1901..2278)
                     /locus_tag="SCK06_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152573.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2578..3459)
                     /gene="galU"
                     /locus_tag="SCK06_002633"
     CDS             complement(2578..3459)
                     /gene="galU"
                     /locus_tag="SCK06_002633"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1152574.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1050 a          692 c          513 g         1209 t
ORIGIN      
        1 ataatttatt agaaaatcac ttttttctat tttgtttttt ttctcctatg tatgattatt
       61 tttttctctt tccatttata ttaagtattt tatttctctg tttttcctta aaattctttt
      121 tttattttta gattagagaa aatctcgtcc tgtttttttc attttacaat cattgaaaaa
      181 ttgaatttca ttgcaaaaaa aataataaac acttataaat atccattatc tatccttcaa
      241 tttttgtgta ttttatttct attagaaaaa ggtatcctgt tggacaattt tgttatatca
      301 ggtaaacttc attgcaataa tgataacaaa tgtactataa ttactttgta aagagttttg
      361 gggaagactc tttcatcaac gtgtattaag agacaagaaa gaatccatat ggattctttc
      421 ttgtctcttt ttccactact ttttattttt ctgacactac atattcttat aactcatttc
      481 tcataactgt gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca
      541 aagatgatcc atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa
      601 taatcgtttt ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa
      661 ctttgtcaac atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta
      721 aaatacaaaa cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct
      781 ggatctagat ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc
      841 aaatcagcaa attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac
      901 aaatcaaatc cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt
      961 tcgatgattg ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg
     1021 acagaaagct gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg
     1081 gctggatttt caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat
     1141 gctacaccac ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt
     1201 ttcgcgatcc aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc
     1261 aattggtgat aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt
     1321 gacatgaata gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca
     1381 gtaacacatg tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa
     1441 taccttgaca tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag
     1501 aattttcacc ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa
     1561 aatgatcggt cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa
     1621 gggtcaggga gccttttttt attcagctgt ttctatctga atttattgta agtaatctct
     1681 tatccaagtg caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat
     1741 aacataaaaa aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt
     1801 ttatataagt gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt
     1861 atccattatt ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc
     1921 ttctactttg ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc
     1981 ctatccgaga ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa
     2041 ataaagcaaa tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa
     2101 ccccaacgga tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc
     2161 gaaacatgat aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt
     2221 ccatcagcca cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag
     2281 tgaacacaca ccttttttta taattaaaaa attcgccttt ttattctact atgaatcttc
     2341 tggcttggct ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt
     2401 aaaccattag cataatattt tctgaatcta ttcacgaaag tctttttcta tctttcgttt
     2461 tccacgactc aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac
     2521 aagctgccgc tttaataaca aatagccttt cattctcagc aggccactta tttccagtta
     2581 actttctgtt cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat
     2641 ttccgaatgt tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac
     2701 gtcgtatctt tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg
     2761 aagtgcatcc gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc
     2821 aaatatttta ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc
     2881 catttcaggt ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt
     2941 aggatcaata attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc
     3001 ggaagcttgt gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt
     3061 catcaaatca tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg
     3121 aagcacggca tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg
     3181 agtcgtttct tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc
     3241 caattcgata tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat
     3301 caagatgtct tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt
     3361 atccacgatt ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc
     3421 tagcccagcg gcaggaatga ctgctttttt gaccttcact tgct
//
LOCUS       DARBER010000174         3420 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARBER010000174 DARBER010000000
VERSION     DARBER010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.179"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="SCK06_002634"
     CDS             complement(<1..2177)
                     /locus_tag="SCK06_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152575.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="SCK06_002635"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="SCK06_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          965 a          755 c          538 g         1162 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgaatcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acacttttat aaaacgacga aaaataaatc actaccagtc aacttctata
//
LOCUS       DARBER010000175         3345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARBER010000175 DARBER010000000
VERSION     DARBER010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.180"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            241..453
                     /locus_tag="SCK06_002636"
     CDS             241..453
                     /locus_tag="SCK06_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152576.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(731..2530)
                     /locus_tag="SCK06_002637"
     CDS             complement(731..2530)
                     /locus_tag="SCK06_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1152577.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2654..3250)
                     /locus_tag="SCK06_002638"
     CDS             complement(2654..3250)
                     /locus_tag="SCK06_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1152578.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1222 a          606 c          589 g          928 t
ORIGIN      
        1 ctcacctctt tataaataac ttagcaatat aaagaaaaaa acgcacgaac aacataggaa
       61 tataaacaaa cgtttttgtc acaacgtcta agatcgtgtt aaaaaatagt tatctaaaaa
      121 gcagaaagat actcaaaata aaaaataaaa aaatggataa aaacgttttc aaatacaagg
      181 taatttgtta ttataaaata aagaaaaaac aaaataaaaa taaagaaaag agggaatgaa
      241 atggtaaaag cagaagtagg aagatcttat atatgcaaac cgatcggagt atctcatgaa
      301 ctaatcggct gtattgaacg tacatatatc aacacggctg tcgtgtatgt tgaaagttat
      361 gaccaacgag actatcaact tatcaatgaa agcaagtatc gcatgctcgt aaaattctgt
      421 gatattagcg cacctgctga attggtttct taaacagtat ttttgagaat tcgcaagaac
      481 tcccttataa tataaataaa gacagaaggg agcgagaaga tatgccaaaa agaaaaagtt
      541 cagggaaaat gggaattatt cttgcagtga tctctgcaat tcctgctatt ttagctattg
      601 ttcgtgcgat gaaggaatca agagaaagtt aagcacacat tatgagcgtc aaacaaaaaa
      661 cgcatatacc tgcggtggca ggttatgcgt tttttgttgt ataaagactt ttttcattaa
      721 cgatttttga ttagccgact cccaaaacaa aatttagcag tttatctgaa taagtgattt
      781 cttcatctgt ttgtttcaca ctttcattta gtagtcccat ctcatcggtt atgataccat
      841 ccacaccata aaacatcatg cgactcatag catctgaatc gttgattgtc catacatata
      901 cttcttttcc gtcttggtgg gcagaattaa tgaagttacg gttgatcgta ctatattcca
      961 ttgtcagaaa atcagcaggc gtcacagggg gcccaacaat attgaaaggt aagatgtagc
     1021 ccacataaaa gtttggttct gcttctttta aatctgaaac ggtttggtac gtcaaacttt
     1081 gcaaaatatg atggttctct aagataactt tgcggtattt atggacaaat cgttcgacaa
     1141 ggtcatccga atctttttta gaggtcttga tctcaataag taatttttgg tttagcttat
     1201 gtgctttttc caaataagaa tcaaaagaac aaacaggggc ttgcactccg ttttcagtaa
     1261 ctgtaagatt tgtcaattct ttcaatgtaa gatcctgtgg tgttccattt acccctgtta
     1321 agtgacgaag atttgcatca tgcatcacta cgaaatgacc atcttttgtt tcctgaacat
     1381 ccatctctac ataatcagga tgatagtctc tgcttgtttt ttcaagtgct tctaaagtat
     1441 tttgcgcccc attttgggta ctcacacctc tgtgagagac agtcactggt tttgtctgaa
     1501 cagctgaagt cagataatcc atattgtaaa gacagacgcc aataccgaaa aaaactgcaa
     1561 aaagagtcaa accagtgttt ttcagcgcag agaaacgcaa gttgggggct tgtgggataa
     1621 accacttagg gatctcaggc aagaagcctt cgtcatccat gaaatcaaca atgatataaa
     1681 aaatccctac ggtagacatc acgatattga ataataaaat cccttgtaaa agcgtcatag
     1741 caaacacagc gctgatcaat gcatagtctg ggaaaaattg ttcaaccata ctttgtgcca
     1801 aaataacaac gatatatcct aaagaagaaa ggagtaaaat cgtaccgcca atcacaataa
     1861 attggcccgt gatggccaaa agacgcgatt tggtcaatga ccagctttcc cgaatggctg
     1921 ctcgaaaagg acgatctcgt agaatcattt ccggtaaagc aaaaatcagt cgaatcccaa
     1981 tatagccaag gaaaatataa acgagtaaaa aggaactgac gatgatccaa cgattagcaa
     2041 aaataaaatc cataatgaaa gcggggattt ttatttttga cagtagatca gaattaaaac
     2101 ttaatccgct gatcggtaga atcaataaaa aataagcaag gaaaaacaaa aaagtaatgg
     2161 gtctcacttt cttcagctga agtatcgtca agtgtaagag ttgcttcaaa gagattggtt
     2221 ctttcttttt aataaaaaaa acactcatca aaagaaaagt aaattcgaaa taaactaaca
     2281 gtaaaatcgt catcaaaact acgattaacg agagtagaac cccaggatgt tgagaaaaaa
     2341 ttgaaggaat attgtcataa gacaaatagt caattgcccc tcggtgtaaa ataaaacggg
     2401 tcgtactgct caataagggt aaacaaataa ataaaataaa gccatgcatc agcaagacat
     2461 cacggaaata agcttgtgtt ccttttaaaa agtcccaagc attacctaag ctacgtttca
     2521 aataattcat cctatcacct gctcttcaca ttgtagcgca aatctaatga aaaaggaaag
     2581 ggtgcagtgt gttttgcaga ccctttccca gatttaacat tgtcaatcaa tgactaattt
     2641 tacaaaataa atattatcca agaagaccac ttagatattg acctccataa atcaaggcca
     2701 tactatataa gaaaatcgac agtggtttag caatcaaaat gattagtgta tatcgtttca
     2761 ctgacatgtt tgtcaatcca gccatcaaac ataacgcatc gtcaggagca actggaagaa
     2821 aaatagctag agcaaacagc ttctcaaaac ggttttgatt atccagccag ccaatatatt
     2881 tgttatatgt cttatcgcta atcatggaaa gtatgaatgg ctttccataa cgacgcccta
     2941 gcagaaaaat gataatcgaa ccaatgcaga ttccaatata gttatagata aatccagcat
     3001 aagggccaaa tatcaggaca ccagctgcag tactgatccc tccaggaatg attggaataa
     3061 ctacttgcag gatttggatg aagacaaaaa taatcggtcc taaaatgatg gaatcaccta
     3121 ctaatcctct taacgcattc aaatctttga aaacacctaa attaataaaa taaatcgtca
     3181 gcgcgattga taggcctaat ccgataatgg atatgaaatt tatcagtttt cgagaggcag
     3241 caatattcat tattcatcaa ctccttagag gttctttgtt aactatactt tactagaaaa
     3301 gcaactaact gcgccatttt ttgatatgac agaaaacaaa caatt
//
LOCUS       DARBER010000176         3345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARBER010000176 DARBER010000000
VERSION     DARBER010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.181"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            100..1032
                     /locus_tag="SCK06_002639"
     CDS             100..1032
                     /locus_tag="SCK06_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1152579.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
     gene            2583..3345
                     /locus_tag="SCK06_002640"
                     /pseudo
     CDS             2583..3345
                     /locus_tag="SCK06_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1213 a          500 c          640 g          992 t
ORIGIN      
        1 ttattacgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
       61 aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      121 acggatgaac ttgtaataat agagtcttat ttcaaaatga atcaatctgt tgctaaaact
      181 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
      241 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      301 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      361 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      421 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      481 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      541 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      601 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      661 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      721 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      781 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      841 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      901 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      961 aaacgaaata atattcctag aaaatcactg gattatcgaa cgattatcga acacctttgg
     1021 aagtattttt gagttacgta agtattgatg atctgtctaa tttaatttga caattaagaa
     1081 agtattaact tgttttgtga aaaagagaat ttgaaaaacg attggcgaac aaatgctcgg
     1141 taggtatggt ataaatacag agctaattgg tgtgatgaaa tcagatattc gcgtgagatt
     1201 cagtaaagta ggagttcgaa aacagctagg tgacaaacag tattgctttg gtacttcgga
     1261 atggtttatc aaattcttat caacagttca cttctcttta tgtttgagtg atgattcgtt
     1321 aggttattgc ttggaaagac cggatgagag tagaagagtt taattttata aagtgaaaag
     1381 aggtaatgta cgatatacgt agggctgaac caatagagga taagcaaacg atgttacagg
     1441 cgcaaaattc atagcttact gttttggaga taacagaaga ggaaataatg cagaagtaag
     1501 gcgaaaaaag gacggaagcc taacagcata tgaaataaag aaaaatatta taactactga
     1561 taaatcggta ttgagcaaag tactaatagg agtcaaaaac attcataagc tatgagtgtt
     1621 tcttggctac ttttttattt ttatgtatct acaaaagttt ccaggggcag aacctgtgat
     1681 tattaatgga agaacctcaa gtattgtctt aaatcattgt tatgaaaatg gattaaaaga
     1741 gttcatcaag gtgtgattaa caagttactt ttcattacat cgaatttttc agaagttgct
     1801 tatattggtg atgatattaa tgatttatca tgtatgacat ctgtgaagaa gcgggaggaa
     1861 tagtagaatg tccaaaaaat gccacaagaa atgagattga attatctgat tttattgtaa
     1921 ctcacagtgg tggtgacgaa tctgtgatag agtttattga gtggatattg gaatcgatat
     1981 agtgtagtag tacagaaaaa ttatcataca attaatacta ggcataagaa tattggcata
     2041 ttctagagtt aaaagtaact attcgaaaag gataaggtta gaattttttg ttttgccagt
     2101 ggaagaatat aaaaagggca ttggaaatta atatattaga atgctaagcg gagaaaaaaa
     2161 tatatgaaaa gtacgtcctt gtgatgggat ggaaaaatac acttatttca aacaacgata
     2221 atataattat gtgaaccgaa gtatactcgc ttctaattca atcctcaaat cccgacagtc
     2281 gcgacttcaa aaaagcgtat agttttaatg tatccccata tgatccgaaa aatggtaatt
     2341 tacaattaac agtttgatga tgttgaagat gtggaaatgc tgaaattacc atgatgagaa
     2401 acacattaat taaagggaat taattaacta gaatataatc tgattcaatt tgaattaagc
     2461 aaaaaaaggc tgagttttaa ataatcgcga attgtaaaat taagttagaa aaataaaaag
     2521 gcatttatgg tacactcaaa ttgtatttcc gacgaaagaa aacaaaggag tgtaacccat
     2581 aaatgaccta caaacatctt accatagacg aactgacaat gatagaatca tattatcttc
     2641 aacataataa gccggttgaa atcgctaacc gaatgggacg tgctatacaa actatttata
     2701 atgtagtcaa taagttcaag caaggcaaga ctgctcttga ttattggcac cagtataaag
     2761 aaaataagaa aaaatgtggt agaaaagtca ttcaattacc tgctcatgaa gtagattaca
     2821 ttaaagagaa agtcactctt ggttggacgc ctgacgtcat tatcgggcga aaagaaaggc
     2881 ctgtttcatg cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg
     2941 acacactacc gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac
     3001 aacagtatca gcgctcaatc catgatcgac ctgataatta tcctcatttc aattctgagt
     3061 ttggtcacct tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt
     3121 tagttgaaag attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag
     3181 atattgaaac tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta
     3241 ttacgctatt acgtttgact gtggaaaaga attttctaac tggaaagcca ttagtaacca
     3301 acatgatatt gatatatatt ttgcggaccc tggaacacct tctca
//
LOCUS       DARBER010000177         3272 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARBER010000177 DARBER010000000
VERSION     DARBER010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.183"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..380)
                     /locus_tag="SCK06_002641"
                     /pseudo
     CDS             complement(25..380)
                     /locus_tag="SCK06_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(640..1188)
                     /locus_tag="SCK06_002642"
     CDS             complement(640..1188)
                     /locus_tag="SCK06_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152580.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1189..2046)
                     /locus_tag="SCK06_002643"
     CDS             complement(1189..2046)
                     /locus_tag="SCK06_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1152581.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2332..2618)
                     /locus_tag="SCK06_002644"
                     /pseudo
     CDS             complement(2332..2618)
                     /locus_tag="SCK06_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2608..2937)
                     /locus_tag="SCK06_002645"
     CDS             complement(2608..2937)
                     /locus_tag="SCK06_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1152582.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            3159..>3272
                     /locus_tag="SCK06_002646"
     CDS             3159..>3272
                     /locus_tag="SCK06_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1152583.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          974 a          651 c          423 g         1224 t
ORIGIN      
        1 tatttaaaac tttgcaacag aaccattaaa tgttcttact gcctcttcca cactaatttc
       61 ttcgttcaat ttcactaata atacttgatc tcgcttatta taatcgcttt catttatcct
      121 aagagtcaaa tctttttcca ccataacttt tacacccaat aaattttgcg gtttattttt
      181 ctctgttgaa attctttgtg tagcctttaa actgacaatt agctcattgg gtacatactt
      241 atttgagagt catctaattg gctcataatc ctttcttttt ggttagtatc tgcagttact
      301 tgtttagtat gagccatgaa cccaaacata ctcaccccaa caaccaatcc taaatatatt
      361 ttctttttaa actttttcat ctacatctcc tcctaatagt tttattttag agttagctct
      421 attacttatt tactaactca acaacagatt atcatacact tgttaatgaa atcacaatat
      481 ctcattaaaa cacaccctaa aatcataacc gttcctatgc tcattttttg gattttttaa
      541 tcacgtaacc agaaaaataa atcccctcta tttcacgcaa atcaaagaag aactaagtaa
      601 ctaccgttta aaggtagtta cttagttctt ctttgattat tatctaactt tattattcat
      661 tccccaccct ttgcgcagtg agtcttcggt atcttgtttt atctgactgg tagttcttat
      721 tatttctcca cgatcatcta attctggaac aattggatat ttttctctga acaagtctag
      781 ttctttcacc acgtcattaa acaaatcttg cttatcttct tgcgtaagat tctgtttaat
      841 atactcatct aaagcttttc taagcaattc atctcttgtc atattagtct catacttttc
      901 tactaaaact ctggaagtcg catttaacaa agtcgacagt tttaaaggat gcttggtgga
      961 ataaacctta tcaaactttc tgattggtgc gccaactttt tttgttcttc ctctactctg
     1021 tctttcaaca ttggaggaag tttttatttc ttgaacttgg tgcacttcta attgcttatt
     1081 ttcagatggt gtaattgctt gctgcttttt ctttgcttct gcttcttttt ttattttggc
     1141 gagtcctttg ttgttcccaa aaatatcaag tcctttgatg tcagccaatt actccacctc
     1201 cgcataccaa ttctcgtgtt cttccaattc tttcaaaata ctgataaata attcgtggct
     1261 tttgcgatcc cattgtttta agagaccttt ctttgtcttc tccatcgtaa tacctgatcc
     1321 gtcgtatcgt tttaagcgcg catttttgag tacaacattt ttaatcaaat gttccccata
     1381 aatctcttgg gcttgtttat acatttcctg atctactgcg tcaccgcttt ccaacatgaa
     1441 aggaataaca ccagcaacat ataaatttgt atcaaattct ttcacaaaaa agtccatata
     1501 ctcaagatat tgcatcgcat ttctcatcga ttttacttgg gtctgtaaga cgacgattgc
     1561 ccaatccgca atgtacatcg cggatttact atatacagaa atagaaggtg ggacatctaa
     1621 atagatgacg tcaaactcat caaataatgg ttctagcttt tctttcatat atttgaattg
     1681 ggataatgaa tcattatatt ggtctttgat ccaatcatca tacatttcaa aggacatgtc
     1741 tgctggaagt atatagaggt tattgattac tttatagaca gcctctttag gtggaagatt
     1801 gtttatcaac acgtcagtca cagtattttc gtaaacaatc ggttcacctt ctccgcctcc
     1861 ttcttcgtta gtgaagttca tatgcgttaa gttcatcaca tcagaagtat ttccttgcat
     1921 atccaagtca attatcaagg ttctttttcc ttcattaact gctgattcaa accctaacat
     1981 ttgcgcgcac gtacttttac ctacgccacc tttaaaatta ccgattacat atttcttcgc
     2041 taccacgttc ctacctcttt tcttttagca atattcattt gatactatta taacatattt
     2101 atttttagtt actaccaaaa aaaagatata tatgtactac cttataatta ataagtaaaa
     2161 ggtagtacat aaataaggaa aagctgaaat atattgattt atagctttcc tcaaagtaat
     2221 gacgttattt aatttatcga gaagcagaga atactcgcga ctttagtcat gtagacgaat
     2281 cagattttcg ataaaaaaga agccgtcctt cttaagaacg acaacaaatc actagattaa
     2341 ttttttcaac tgttcataaa agttttcatg cgttccacat aacaatactg gaataacctt
     2401 gttttctata atctcgtaag ctactctata tgttgtgccc gcatatttga acccactaga
     2461 ccaaatacct gataaatccc cagttttttg ttcaccctta tatggatcaa tcgcaatttc
     2521 atcataaatt aaattcaaaa atttttcttt caatggcttc tcttttagct ttttaaatac
     2581 ttaatgacat gttttgaagg aacgatttca taagccaatt tctgttgcca actcctcttt
     2641 agtcattact ttagaattag taagtgtatc ttcagcaata tctctaaagg aagaatgcat
     2701 tttgtttttt ctattcttaa attctacaag aatctcatct ttatcgtagc cttctgcaat
     2761 tatatcagac aaaatatctt cgctaaaatc ataaaattct ttttgtggtt caacaaattc
     2821 ataaagaata ccattctcta ccaactttgc ttcaacttcg actccattag gaacattaaa
     2881 atctttaggt actgtcaaca tgattgaatt gccttgtttt cttgtttttg taatcattta
     2941 aatccctcat ttctaacagt atgtaattac ttagtaaata cattatacca cgagataatg
     3001 tatttacaag taattactgt gactcgtaat gcgtccttgc tgaataaatt tcaaccacgt
     3061 gttcatcttc atctacctta taaacggttc tgttgcaaag ttttccaaaa aatctatttt
     3121 agtgtaaaat tgagaaaaaa gacagagagg acagagtaat gaatcatttt aaaggcaaac
     3181 aattcaaaaa agacgtcatt attgtcgctg ttggttacta cctgcgttac aatctaagct
     3241 atcgtgaagt tcaggaattg ttatatgatc gt
//
LOCUS       DARBER010000178         3256 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARBER010000178 DARBER010000000
VERSION     DARBER010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.184"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(14..706)
                     /locus_tag="SCK06_002647"
     CDS             complement(14..706)
                     /locus_tag="SCK06_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1152584.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="SCK06_002648"
     CDS             complement(1143..1712)
                     /locus_tag="SCK06_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1152585.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="SCK06_002649"
     CDS             complement(1786..2109)
                     /locus_tag="SCK06_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152586.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="SCK06_002650"
     CDS             complement(2263..3075)
                     /locus_tag="SCK06_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152587.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          913 a          652 c          556 g         1135 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataag
//
LOCUS       DARBER010000179         3211 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARBER010000179 DARBER010000000
VERSION     DARBER010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.185"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            180..749
                     /locus_tag="SCK06_002651"
     CDS             180..749
                     /locus_tag="SCK06_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1152588.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(927..1367)
                     /locus_tag="SCK06_002652"
     CDS             complement(927..1367)
                     /locus_tag="SCK06_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG1152589.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1525..2289
                     /gene="map"
                     /locus_tag="SCK06_002653"
     CDS             1525..2289
                     /gene="map"
                     /locus_tag="SCK06_002653"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG1152590.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2321..>3211
                     /locus_tag="SCK06_002654"
     CDS             2321..>3211
                     /locus_tag="SCK06_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG1152591.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNK"
BASE COUNT         1016 a          551 c          652 g          992 t
ORIGIN      
        1 ggctttaaat aaaaaaagga ggttattttt ttaaggttaa gtttcatgat atatgttact
       61 acttgctatt tcagcttgtg aagaaaatca aaaactaatt ttgcatatca aaaaaacatg
      121 ttacaatgaa attaatcaat ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata
      181 tggcaagaaa aaagacgatt actagagaac agattttggc agctgcttat gaagtagctg
      241 ccaccgatgg atttacacga tttactgcga gaaacatcgc gaacaaaatg aattgttcca
      301 cgcaaccgat ttatcttgag tttaaaaata tggatgactt gagacaagct ctttttgagc
      361 agatctatga tcatttacaa tatgaagtgt tcactgttga gcatacaggg aatacgatta
      421 tcgatcttgc ttttaattat atccattttg caagaagaga gaagaagttg tatcgttcac
      481 tgtacttaga agaatatggt ggaggaaaag agatgcagaa tttctcttat acttactttg
      541 tgaatacagt gaaacgcgat ccagcttatg tgaatctttc agaagagcag atcgaatctt
      601 tgtataatgg gacatggatc atagctactg ggatcgcagc tttgatgtct tcaagcatca
      661 tccatccaac tgatgaacag attgaaaaga tgatccaaga cagtatagat gctatcttga
      721 aattcaaaga accaatcgaa atcgattgaa tatactcaaa agttccacaa atcagtaggt
      781 aacaagagat gtgtcaaaat aaggaaaagt tcgtttttct tacagcgtat gcatttttaa
      841 ctcccgaaga actcttccat gaatgcaatg aataaaagag ccatacagat tgaataaatc
      901 tggatggctc ttttattgca agtatattaa atttttgcga ctagttcttt agcgaaagct
      961 tctaaatttt cgatatcttc ttcttcagca ttcagatcca ctttaacatt ttcggctcct
     1021 tttgatgcgc ctgtttttgc aaaagcagca tcgaaatcat ccaccgattt gcagaattca
     1081 tcgtaaaaag tatcaccaga tccgcagaca ccgtaaattt ttcccaatag atccaattct
     1141 tgcaagtctt cataaaagtc tacgatctca tcaggcagat cgccatcatc gtatgtataa
     1201 gtagctacta cgcaaatatc tgcctcctcg aattcatctg catctacttg ggtacattcg
     1261 ttgatttcga cttcgatatc cagattttca aatgcttctg caacaatatc ggcgatttct
     1321 tcagtgtttc ctgtcatgct agcatatacg atttttgcta aagccatgat gtgcctcctc
     1381 gtttataatt atttaacaat tatgaattat accaaaacat cattgaaaag aaaataaaac
     1441 ctaaagacac ctggggaatg tcatttggct aaatgtatgc cgctatgtta aaatgagagg
     1501 gatttgtaaa aaggagacga aaatatgatt actttaaaat cagcacgtga aattgaaatg
     1561 atggcagaat ctggagcttt attagcagat gtacataaaa atctgcgtga ttttataaaa
     1621 ccaggaatca caagttggga tattgaggta tttgtcagaa actatatcga gagtcatggc
     1681 ggaattgctg cgcaaattgg ctttgaaggg tatgaatatg cgacttgtat cagtataaat
     1741 gatgaaattt gtcatggatt ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa
     1801 gtcgatatgt gtatcgattt gaaaggcggc atttctgatt cttgttgggc ctatgtagtt
     1861 ggagaatcta cacctgagat cgaccgattg atggaagtaa cgaagaaagc tttgtacatc
     1921 ggtattgagc aagcacaagt agggaatcgg attggtgaca tcggtcatgc tatccaaact
     1981 tatgtagaag gcgaaaatct agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct
     2041 acgattcatg aagaaccagc agttcctcat tacggagaag ctggaaaagg gttgcggttg
     2101 aaagaaggca tggttatcac gatcgaacct atggttaata ccggaacttg gaaaatgaaa
     2161 atggatccta acggctggac tgcgtataca agagacggcg gactttcttg ccaatatgaa
     2221 catacattgg ctattactaa agatggtcca agaattttga catcacaagg agaagaaggc
     2281 acttattgat ctatccagtc aataggtacg ggagaggtac atgaaagttt ggaacaaaat
     2341 aaaacaaaat gaaaatctga tgcgctttat tgagacgact caaaagcgca tcactgattc
     2401 agaaatgagt acgacctctg tcgtcgttgc ttattattta ctcttgtctt tatttccatt
     2461 gattattgct tttggtaata ttttaccgtt tctccacatc gatcaagaga ctgtactgac
     2521 ctatttacga gaagtgattc ctgagaccgt ttaccagttt atcggtcctg caatcaaaga
     2581 ccttttgaca caaagttcag gaggcttgtt atccgtttct gcattagcgg ctttatggtc
     2641 tgcaagtcaa agtatcaatg ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa
     2701 tagaaaaaat tttttgatcg tccgtttctt ctcactaatt gtcattgtct tgttcatgat
     2761 tgctattagt ggggtaacta tcgttcttgg gttgggacag ctgattttgg atgctattca
     2821 gccaatcgta caaatacccg tgaactttat cgatcagttc caaactttga agtggccaat
     2881 cacattggta gctttatttg tcatcatgtt cttgatttat ttaattgtcc ctaatgctca
     2941 gttgaagttg aagacgatca ttccgggcac gatcttcgct actgtcggct ggatgctgtt
     3001 atcacaagtg tttagtatct atgctaggta ttttgcaacg aggatcagcg gttatcagat
     3061 cataggaagt tttattgtac tcatgctttg gcttaatttt gctgctacga tcatcatttt
     3121 gggcggaatc atcaatgcag ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg
     3181 tcgagatcca agccgaaaat ggttcaataa a
//
LOCUS       DARBER010000180         3199 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARBER010000180 DARBER010000000
VERSION     DARBER010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.186"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            301..585
                     /locus_tag="SCK06_002655"
     CDS             301..585
                     /locus_tag="SCK06_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152592.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="SCK06_002656"
     CDS             588..1004
                     /locus_tag="SCK06_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152593.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="SCK06_002657"
     CDS             complement(1141..1860)
                     /locus_tag="SCK06_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1152594.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="SCK06_002658"
     CDS             2513..2608
                     /locus_tag="SCK06_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1152595.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="SCK06_002659"
     CDS             complement(2910..3155)
                     /locus_tag="SCK06_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152596.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DARBER010000181         3195 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARBER010000181 DARBER010000000
VERSION     DARBER010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.187"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..466
                     /locus_tag="SCK06_002660"
     CDS             <1..466
                     /locus_tag="SCK06_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1152597.1"
                     /translation="DTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(552..1646)
                     /locus_tag="SCK06_002661"
     CDS             complement(552..1646)
                     /locus_tag="SCK06_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1152598.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1835..2596
                     /locus_tag="SCK06_002662"
     CDS             1835..2596
                     /locus_tag="SCK06_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1152599.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2711..3151)
                     /gene="fabZ"
                     /locus_tag="SCK06_002663"
     CDS             complement(2711..3151)
                     /gene="fabZ"
                     /locus_tag="SCK06_002663"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1152600.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         1021 a          635 c          539 g         1000 t
ORIGIN      
        1 tgatacgatc gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc
       61 taaagtcatt atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct
      121 taatcaatgg ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg
      181 aaaagaattt tctaactgga aagccattag taaccaacat gatattgata tatattttgc
      241 ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg
      301 taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta tttccagtgt
      361 cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac caatcgaaat
      421 atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa tcataaccta
      481 taaaaaaaca aaataaaaaa gcagaaataa ctggctattt ctagcggatt atttctgcaa
      541 taactaatta tctatttttt attttcaatt aatttaaagc ttgaccatgg tttcaaaaag
      601 tcaaggagga ataattcgtc atcggagatt cttcctacaa cattgacacg tccgtcgttt
      661 ttcatatcct tcaaggcgat ttgtgtttct cctttgtatt gaccgtaccc ttcattgtca
      721 atcagtacat ccccatgatg catatctcgc gtattatgag gcgggaaatc tttatctttg
      781 tagtatacac gagtcatcgt tgaacgaagg atatattctg agcgatcccc tcgataacta
      841 tgaaggttat caaacaagca gattcgttcg ttttctgtaa taccttcttc cgtatctact
      901 ttcaatgttg gataatccgc gttaaatgct tctgccattt ctttcaattc ttcttcactt
      961 gcgtacgcat ttccaataga aatatcatca atcaatcctg taaggattaa atgcttcact
     1021 tgtgtcgcaa tttcaagatc tcggtggtct tccagcgtgc acaagccatc ttgcgtaggc
     1081 caagggccaa actcagcaga ttgcgaattg acgaatgcca tcgtattcaa attatatttt
     1141 ctgaattttt ctgaacaaaa aacaaagtgt tcgtatccta aaccagagta tcgatgagga
     1201 taaaaattat gagaacctaa taaattatcc gtgtttggcg aataactcat gatgttgtca
     1261 acgtaattcg tgcccgaact catattgatc tcgattttga taccaaaagg attgcgcgtc
     1321 atttttgctt cttctgctcc tgtgaatccg atatctaagc ggaccccgta ggcacccatc
     1381 ttatggaaaa aagataagtc atcgtaagaa atctccaact gttcaaataa cgctggattg
     1441 atatcaacca ttacttccat gcctaggcta ttcgcgtaat caactacttc tttaaattct
     1501 gaaagaactt tttctttatc atcattgatc tgcagcaaac tggtaaaaac ccgtttatac
     1561 ccatacttat gagctaaatc caagtacgct ttgtcctttt caaatgttga ccgttctgga
     1621 taaatcgaga ttcctaattt tcccatatta ccatttcctc ctgcatcttt tcgtttttgt
     1681 atccacttac attatagcaa acacaaagta aatggtctac accttttctt tttcttttaa
     1741 aataaatgat aaattccaat aatttaagta ggtattttcc ctctattttg ttatcattaa
     1801 agataacaaa atagtaaatt ggaaaggaac agttatgaag aaaaataaag ttattttaag
     1861 tattagttta gcagcagcat tgctgtttgc tgctggttgt tctgcaaaca acacgacttc
     1921 ttctagttcg tctacagaaa gttcaaaaag tacatctact gcggaaagca cgactgccag
     1981 tagcgtcgat ttgaacgcat tggaattacc acaattgacg aaagatgtcg ccgaaaatga
     2041 agatctggtc gaaatggtga cgaataaagg cacaatcaaa atcaaattat ttcccgaaca
     2101 cgcaccaaaa gcggttgaaa actttatgac acatgcaaaa gacggttact acaacggact
     2161 gactttccat cgcgtcatca aagatttcat gatccaaggt ggcgatccta aaggcgatgg
     2221 cactggaggg gaaagcattt ggggagaagg atttgaaaca gagatcaaca accaccttta
     2281 taatataaga ggtgcgttat ctatggctcg ctcacaagat cctaacagta acggcagcca
     2341 gttctttatc gttcaaaata cagatgatat gcatgatggt cttttaaaag acgactatcc
     2401 tcaagcaatc atcgacgctt ataaaaacgg cggttatcct tcactagatg gccattatac
     2461 tgtcttcggc caagtgattg aaggaatgga tgtcgtggat gctattgcaa atatcgaaac
     2521 agatagttca gacaaaccaa aagaagaagt aaaaatcgat aaaatcaata tactgcaaga
     2581 agcaaaacaa aaataaaaaa ataacgtctg aatcctgcat agatgacaac gtatgctagg
     2641 attcaggcgt cttcttttta cataaaaagc aagtaagctt ccttttgttt tgctatatca
     2701 ggttttatag tcaagcattt tggttgacga tgaacgtgaa ctcgcactca cagacttttt
     2761 tatcatctac atatgctgca gataacgctg taccgactga tcccttcatt ttggtgattt
     2821 caaaatgaca tttcagcaca tctcctggaa caactttttg acggaatttg gccttattga
     2881 tcccaccgat ataagctctt tttcctagaa actcttctga ttttaggata agtatcgaac
     2941 ctgcttgtgc cagtgtttct agaatcaacg cacccggcat cactggattg tcaggaaagt
     3001 gaccttggaa aaaatcttca ttgattgtta cattttttgt cgcaacgatc ttttttcctg
     3061 gttctaaagt atctacataa tcaatataac aaataggata acgattagga atcaattcca
     3121 ttacttcagt agcagataat acgtaagaca taattaaact ccttctttca ggcaattgcc
     3181 attttcatta ctttg
//
LOCUS       DARBER010000182         3129 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARBER010000182 DARBER010000000
VERSION     DARBER010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.189"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            103..1347
                     /locus_tag="SCK06_002664"
                     /pseudo
     CDS             103..1347
                     /locus_tag="SCK06_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="SCK06_002665"
     CDS             1360..1992
                     /locus_tag="SCK06_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1152601.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="SCK06_002666"
     CDS             2197..2424
                     /locus_tag="SCK06_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG1152602.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="SCK06_002667"
     CDS             2493..2759
                     /locus_tag="SCK06_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1152603.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="SCK06_002668"
     CDS             2749..3018
                     /locus_tag="SCK06_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1152604.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1102 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttta
//
LOCUS       DARBER010000183         3040 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DARBER010000183 DARBER010000000
VERSION     DARBER010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.190"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1077
                     /locus_tag="SCK06_002669"
     CDS             202..1077
                     /locus_tag="SCK06_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152605.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="SCK06_002670"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="SCK06_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="SCK06_002671"
     CDS             complement(1642..2973)
                     /locus_tag="SCK06_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1152606.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         1072 a          553 c          466 g          949 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa
//
LOCUS       DARBER010000184         3013 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARBER010000184 DARBER010000000
VERSION     DARBER010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.191"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            177..359
                     /locus_tag="SCK06_002672"
     CDS             177..359
                     /locus_tag="SCK06_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1152607.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="SCK06_002673"
     CDS             462..746
                     /locus_tag="SCK06_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152608.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="SCK06_002674"
     CDS             762..998
                     /locus_tag="SCK06_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152609.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="SCK06_002675"
     CDS             1020..1274
                     /locus_tag="SCK06_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152610.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="SCK06_002676"
     CDS             1356..1541
                     /locus_tag="SCK06_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEG1152611.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="SCK06_002677"
     CDS             1608..2003
                     /locus_tag="SCK06_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG1152612.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="SCK06_002678"
     CDS             1993..2370
                     /locus_tag="SCK06_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152613.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="SCK06_002679"
     CDS             2392..2766
                     /locus_tag="SCK06_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152614.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DARBER010000185         2984 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARBER010000185 DARBER010000000
VERSION     DARBER010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.192"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1769
                     /locus_tag="SCK06_002680"
     CDS             <1..1769
                     /locus_tag="SCK06_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1152615.1"
                     /translation="ELDSHQFNEFRNSIRLSDYEFYYTSKSNLNFCINENYDKLVGRL
                     NDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALRDVLREMKNNRNPVKRIMETI
                     ESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQLNSIIGSVYSPNLLLSSTM
                     SDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVEFEACRSRELLNIMLIEEP
                     EAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEISRMNVLKSFNGKSIVMNP
                     ANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVILVEGDAEEILIPQIVKK
                     LFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTDLDEQAIPSEHRLYKST
                     AQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKENILTYVSPIIDDAFKDK
                     KTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILNAIAF
                     ACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYFGKDE
                     DVVYLINQIENYTKILYAEVSK"
     gene            1766..>2984
                     /locus_tag="SCK06_002681"
     CDS             1766..>2984
                     /locus_tag="SCK06_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1152616.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIAN"
BASE COUNT         1134 a          405 c          500 g          945 t
ORIGIN      
        1 ctgaattaga ctctcatcaa tttaacgaat ttagaaattc tattcgtcta tccgactatg
       61 aattttacta cacatctaaa tcaaatctaa atttctgtat caatgaaaat tatgataaat
      121 tggtaggaag actcaatgac aatatagcta gtaatcctga agaagacgat gaagctctgt
      181 tgggctcaag aattgacatg tcagatattt tcaagcatat ttcggttgtt tatgttgacg
      241 ctcttcgaga tgttcttaga gaaatgaaaa acaatcgaaa cccagtcaaa agaatcatgg
      301 aaacaattga atcaaaaata tcatcagata atgttgagtc attaaaaaca attatacagc
      361 aattaaatga gactattacc agtgttcctg aaattaggaa aattggcgaa aatattaatc
      421 ggcaactaaa tagcataatt ggttcagtgt attctcctaa tcttcttctt tcctcaacta
      481 tgtcagatga tatgggttct ttagcaaaat ttctttcaat gaagccagaa caaaatatcg
      541 acttagactt actagggtta ggacatctaa atatgattta tttagctttg aaaatagtcg
      601 aatttgaagc gtgtcgttca agagaactac ttaatattat gttgattgaa gaaccagaag
      661 ctcatattca tcatcatatt caaaaaactc tgttcgaagg attaaatctt caaaaaaact
      721 atactcagat tctcatgacc acacattctg tacatttagc tgaagtgtct gaaatatctc
      781 ggatgaatgt attaaaatcg tttaatggca aatctatcgt tatgaatcca gcaaataatc
      841 tgaactcatt tgctaaagaa aagttaaaaa agaacaatct aaatctaata aacgcagtag
      901 agagatattt agatgttaaa agaaatggat tgcttttttc taaaggtgtt atcttagttg
      961 aaggagatgc tgaagaaata cttattcctc aaatagtaaa aaaattattt ggtataaact
     1021 tagacgaact tggaataggt ttgattaatg ttggtagtac ttcgtttgaa tatatcgcta
     1081 gcttgtttga cgatgataga atacagagaa agtgtgctat tgtaactgat ttagatgaac
     1141 aggcaatccc atcagaacat cgattgtata aaagcactgc acaagagaaa ggtcaagcaa
     1201 gaaaagaaaa attatcctcc ctttttaatg aaaacaattg ggttaattgt ttttatgcta
     1261 atacaacatt tgaaattgag tttttagatg tgaataaaga aaatattcta acttatgtca
     1321 gtccaattat agatgacgca ttcaaagata aaaaaacaat aactgcttat aaagaagaaa
     1381 tacaaagtga acaatattta gaaagaaatt tagcgatgtt aaaactagcc aatcatgttg
     1441 gtaaggggtg gtttgcaatt gagttaggtg aaaaaattga ctttttaatc aaaattccta
     1501 agtatattct taatgctatt gcttttgcat gtcaggaaat agtagatctc tcaatttata
     1561 aaaaaattct actccataca atcgacttgt acgaggaaac tacagaattg tcagctttat
     1621 cagctagttt aaaaacaagt gatacagctg atttgaatat agcaataatt tctttattag
     1681 agtactttgg taaagatgag gatgttgtgt atctaatcaa tcagatagaa aattacacaa
     1741 aaatacttta tgctgaggtt tcaaaatgac aaatatcttc gaaaaaatcg taaatgattt
     1801 aaatttagaa caaaaagatg ttgtggaaag tgaagataac ctttatgtaa atgcatgtcc
     1861 tgggagtggg aaaacaaggg tacttacaag gaaaattgct taccaagcaa ttaaacacga
     1921 tggatcacta aaaagaataa ttgctatcac atacactaat agagctgctg aagaaattaa
     1981 ggaacgccta agtttactta gtatagatga tgatattaat atttgggtgg ggacaataca
     2041 tcagttttgt cttgaattta ttatttatcc ttttaaaatg aatctctcaa gagtatcaaa
     2101 aggatttaca attattgatg attatactca aagagcttac gtcagccaaa ttaataatat
     2161 gctgaattta aatctcaaaa cgtatgaact gaacaaaatt gatttgtcac tttctcatga
     2221 ttttaaaatt aatgaagttc agtatactca agtttctgaa atgtatcaca ctatgttgga
     2281 aaaaaataaa gaaattgatt ttgatatgat tttactagta gcgtataaaa ttttaactgt
     2341 aaacagtaac gttgcacgta atattgctag taatataagg gcgatttatg tggatgaatt
     2401 tcaagatact agagaattac aatacaatgt aatagctaaa ttacttcaaa acaaccctca
     2461 aattcagtca atgtttgttg gagatattga ccaagcaatt tatggatcat tagatggtat
     2521 agctaaatca gttgaagaac tagaaatgct aactagtcac acttttcaat caaagacgct
     2581 acatggctgt tacaggtcta atcagagatt agtagacttc tactctaatt ttcagagttg
     2641 tccttacgag attgaaagca gagggaataa taaaaacgac cgtggtttta taagttatga
     2701 ttgcaaagtt actaaggagg atttacccaa cataataaaa aatataataa aggaaaagat
     2761 tgaaagcggc attcatgaaa aagatatttg tgtacttgct cctcaacact atcctctgtt
     2821 ttcactgggt gagtatttaa agaaagaatt accgtattgc aattttgatg ctataaatat
     2881 atcaccaatt aaggtaaata atcatagttt atttttcaag ttagcaattt tgtactttac
     2941 tgaatcaggt gaaaaggttc gaagaagaaa gctaattgca aacg
//
LOCUS       DARBER010000186         2854 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DARBER010000186 DARBER010000000
VERSION     DARBER010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.193"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(125..862)
                     /gene="erm(B)"
                     /locus_tag="SCK06_002682"
     CDS             complement(125..862)
                     /gene="erm(B)"
                     /locus_tag="SCK06_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1152617.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(987..1070)
                     /locus_tag="SCK06_002683"
     CDS             complement(987..1070)
                     /locus_tag="SCK06_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1152618.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            complement(1119..1202)
                     /locus_tag="SCK06_002684"
                     /pseudo
     CDS             complement(1119..1202)
                     /locus_tag="SCK06_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            complement(1612..2406)
                     /locus_tag="SCK06_002685"
                     /note="aph(3')-IIIa"
     CDS             complement(1612..2406)
                     /locus_tag="SCK06_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1152619.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(2499..2750)
                     /locus_tag="SCK06_002686"
                     /pseudo
     CDS             complement(2499..2750)
                     /locus_tag="SCK06_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          859 a          573 c          458 g          964 t
ORIGIN      
        1 ttttctaatt tcattttgag gtgaattaat tggtagttat tagaggtgcc ctatacctaa
       61 taatttatct acattccctt tagtaacgtg taactttcca aatttacaaa agcgactcat
      121 agaattattt cctcccgtta aataatagat aactattaaa aatagacaat acttgctcat
      181 aagtaacggt acttaaattg tttactttgg cgtgtttcat tgcttgatga aactgatttt
      241 tagtaaacag ttgacgatat tctcgattga cccattttga aacaaagtac gtatatagct
      301 tccaatattt atctggaaca tctgtggtat ggcgggtaag ttttattaag acactgttta
      361 cttttggttt aggatgaaag cattccgctg gcagcttaag caattgctga atcgagactt
      421 gagtgtgcaa gagcaaccct agtgttcggt gaatatccaa ggtacgcttg tagaatcctt
      481 cttcaacaat cagatagatg tcagacgcac ggctttcaaa aaccactttt ttaataattt
      541 gtgtgcttaa atggtaagga atattcccaa caattttata cctctgtttg ttagggaatt
      601 gaaactgtag aatatcttgg tgaattaaag tgacacgagt attcagtttt aatttttctg
      661 acgataagtt gaatagatga ctgtctaatt caatagacgt tacctgttta cttattttag
      721 ccagtttcgt cgttaaatgc cctttacctg ttccaatttc gtaaacggta tcggtttctt
      781 ttaaattcaa ttgttttatt atttggttga gtactttttc actcgttaaa aagttttgag
      841 aatattttat atttttgttc atgttatcac tcctgaagtg attacatcta taaacaaata
      901 cagaagttaa acgatttgtt tgtaatttta gttatctgtt taaaaagtca taagattagt
      961 cactggtagg aattaatcta acgtatttat ttatctgcgt aatcactgtt tttagtctgt
     1021 ttcaaaacag tagatgtttt atctacataa cgcatttgga ataccaacat gacgaatccc
     1081 tccttcttaa ttacaaattt ttagcatcta atttaacttc aattcctatt atacaaaatt
     1141 ttaagataat gcactatcaa cacactctta agtttgcttc taagtcttat ttccataact
     1201 ttagggttaa ccatacgcaa gaccaatcac tctcggacaa tactcatgat tttaatgata
     1261 aaatccatgt taaacccata gataagaaat acacctgcaa taaccgttac ctgtttgtgc
     1321 caatttaaaa atgcaccact tttttataat taaaacggtt gaaaactgta ccactaataa
     1381 ctcacaatag agagatgtca ccgtcaagtt aaatgtacaa aataacagcg aaatttttaa
     1441 atctatttct tatcgataca aattcctcgt aggcgctcgg gacccctatc tagcgaactt
     1501 ttagaaaaga tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta
     1561 gatgtctaaa aagcttgtag ttaaagcttt ttagacatct aaatctaggt actaaaacaa
     1621 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
     1681 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
     1741 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
     1801 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
     1861 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
     1921 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
     1981 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
     2041 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
     2101 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
     2161 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
     2221 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
     2281 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
     2341 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
     2401 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
     2461 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
     2521 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
     2581 ttgaaaccac aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc
     2641 attagtccat gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg
     2701 cctatgcctt gccccctgaa atccttacat acggcgatat cttctatata aaagataacc
     2761 cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa
     2821 aaaaacgcat atagtagtct caaaactccc aaga
//
LOCUS       DARBER010000187         2844 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DARBER010000187 DARBER010000000
VERSION     DARBER010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.194"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            144..494
                     /locus_tag="SCK06_002687"
     CDS             144..494
                     /locus_tag="SCK06_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1152620.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="SCK06_002688"
     CDS             908..1726
                     /locus_tag="SCK06_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1152621.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="SCK06_002689"
     CDS             complement(1799..2158)
                     /locus_tag="SCK06_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1152622.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          969 a          399 c          505 g          971 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta
     2581 ttagttcata atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt
     2641 gacgaatgta aacgatactt ataaagtaat agaaattgat tacatttgta aacttaaaat
     2701 ggttaattta tctcacataa aactaagaat aaaataatga ttaaaagtgg ttagttttta
     2761 tacgatttag cctatcggta aaaatgaatt agagagtgta aaatattttg tgtaaatgaa
     2821 aaaatccata caaaaaagga agtc
//
LOCUS       DARBER010000188         2826 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARBER010000188 DARBER010000000
VERSION     DARBER010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.195"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            798..1052
                     /locus_tag="SCK06_002690"
                     /pseudo
     CDS             798..1052
                     /locus_tag="SCK06_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="SCK06_002691"
     CDS             1122..1634
                     /locus_tag="SCK06_002691"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152623.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="SCK06_002692"
     CDS             1631..2668
                     /locus_tag="SCK06_002692"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1152624.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DARBER010000189         2822 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARBER010000189 DARBER010000000
VERSION     DARBER010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.196"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            9..1049
                     /locus_tag="SCK06_002693"
     CDS             9..1049
                     /locus_tag="SCK06_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1152625.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            1068..2153
                     /locus_tag="SCK06_002694"
     CDS             1068..2153
                     /locus_tag="SCK06_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1152626.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            2186..>2822
                     /locus_tag="SCK06_002695"
     CDS             2186..>2822
                     /locus_tag="SCK06_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1152627.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYL"
BASE COUNT         1080 a          352 c          521 g          869 t
ORIGIN      
        1 gcgtaaaaat gaacaatccg aaattcagca taatcgtacc agtatacaat gcagaagcaa
       61 ctattggtcg aacgattgat attctaaaag aattggatta tgaagagtac gaagtcgttt
      121 tagtaaatga tggatcaaca gatcaaacac aggaaatcat tcaagaactg attgaaaatg
      181 atgaacgttt tgaattgatt actacgacca atcaagggcc cggattagca agaaacgaag
      241 gaataagaca gtcaaaagga gaatatctct tattcttcga tgcagatgac acgccagtaa
      301 aaacgatctt gtcagattat gatcatttgc tagaaaagag tccaaaagcg gatcttgttg
      361 ttagttcgtt tgtttttcgt acaatggacg agggaagaat cgtttccgaa aaagaaaact
      421 tagttacaga atgtcactat ctaactcacg aagagtttat aaaagacatg tatcagttaa
      481 tgaatgaaca gttgatgtat gttgtatgga acaaatgcta tcgtagcgat attctgaaaa
      541 ataaccaaat tctatttaaa acttataata gttgtgagga tcgaatattt aacttacatt
      601 attatgaata ctgtcatcaa gtgatcatga acccaaaagt agaatatatt tatgaatttg
      661 aaggtgggaa ggggatcact aatcaatacc gacctaacaa gttcgaaaca tttaaagaat
      721 tctattcatt agcaaatact gtaacagacg atattaataa agacggtatg gcggcgcttc
      781 ttttaaaagg gacaacttca gttatttttt ctatttatag tacctctgca agaacttcaa
      841 aagagaagaa agacgaagca aagcagatat tgagtgatcc agtgatccga gaagccaagc
      901 aaattgctaa gacggattct tcagcaaaaa aaataacaaa gttgctttac aatatgccag
      961 ctccactatt tttatctgct gtaaaagttg gaagcttcgt tgaagtgaaa atgccagggt
     1021 taatggctgc tttgaagcga gtatattagt taatggagga aaatataatg aaaagaattt
     1081 tgcattttca gggacgaatg ggtttaggtg gagcagagtc tttcatgatg aatttatatc
     1141 gtaaaattga tcgtacaaac tatcaatttg attttttgat ttatgaagat tatgcagatg
     1201 ttcaagacta tcattcagag attgaaagat taggaggccg tatttttgtt gtacctaatc
     1261 ctaagaaaaa tatttttaaa tatttaatag aagtgaataa gttactaaaa aaagaatctt
     1321 tttctatagt tcacaatcag gtttattttg gcgggggaat taatttatgg ttagccaaaa
     1381 agaatggaat cagacagaga attgctcata gccatgcaac tgaagatgga aaatctcaaa
     1441 atattgtaat gaatgtattg cgtaaatttt taaccaaatt gttgttacaa aatgcaacag
     1501 actaccttgc cgttagtcaa gaagctggtg aaagtctgtt tcaaaaccat ccctttgaga
     1561 tagttcataa tggtattgat ttagagcttt atagtaaaaa ttcagaagct aaggttaata
     1621 agagaaaaga attggatatc tcaatgagta cttttgttgt gggaaacatt ggacgattag
     1681 agaaacaaaa aaatcaatct tatttgttag agattttttt agaaatacta aaaatgaatt
     1741 ctgattctta cttgatttta gtaggtgaag ggtcacttag aaataaactg aaagccaaag
     1801 cagtagaatt ggatatagag aaaagagtaa aatttttagg agaaagaaac gatattcccg
     1861 agttattatc tgtgatggat gtttttgtaa tgccttctct ttatgaaggt ctgccaatgg
     1921 ttggattaga agcacaagca gcaaaattaa aattagtctt agcagatact atttccaaag
     1981 atacaaagtt aacaaataat gtggattttg tatcattaga tgaaacacca caagaatggg
     2041 caaaaaaagt aataaaagag ccgtatgcta ataaaataac acaagaacta aagagttatg
     2101 atgtttcgta cactactgaa caaatggaga gtatatatag tagagatcat taaaaatgtt
     2161 caagatattt taggagtgat tatttttgaa aatatgttat ttgatattgg catacaatga
     2221 tattggcaac ttaaagagat tggtcaatcg attgaacgaa aatgcagttt ttattattca
     2281 tatagataaa aaaactgata tagaaccatt tattgaagca tttaaatcat atgataatgt
     2341 tgaattttta tgtaataaaa accgtgttaa aatttattgg ggtggttttt ccattgtaca
     2401 agcagaaatc aatttagtaa agagagcatt acaaaatgag aaatatttaa aatatgtatt
     2461 attatctggt gctgattatc caatcaaaga taatgagtat atttataatt attttaaaaa
     2521 gaatagttca gtggagttta ttcgtggaat agatctagat caaattaaac ataaagaatt
     2581 ttattataaa catatagatg tatatcaaaa acatgattat cctagaatta ataggaataa
     2641 cacaactgca tttaaaattt ttagagctat aattaatcgt tgtctgagga tgattaaact
     2701 tcccccaaaa attagacacc ataaatttga tttgtatcat ggttctcaat ggtgggcttt
     2761 gtcgaaagaa tgcctaacag aattaataca aatgtatgaa caaaatcaac aagactacct
     2821 ga
//
LOCUS       DARBER010000190         2818 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARBER010000190 DARBER010000000
VERSION     DARBER010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.197"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..273)
                     /locus_tag="SCK06_002696"
     CDS             complement(<1..273)
                     /locus_tag="SCK06_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1152628.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKP"
     gene            complement(273..1250)
                     /locus_tag="SCK06_002697"
     CDS             complement(273..1250)
                     /locus_tag="SCK06_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1152629.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            complement(1263..2405)
                     /locus_tag="SCK06_002698"
     CDS             complement(1263..2405)
                     /locus_tag="SCK06_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG1152630.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            complement(2395..>2818)
                     /locus_tag="SCK06_002699"
     CDS             complement(2395..>2818)
                     /locus_tag="SCK06_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEG1152631.1"
                     /translation="KKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINP
                     DAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKI
                     GSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
BASE COUNT          893 a          544 c          354 g         1027 t
ORIGIN      
        1 aggtttttct aaaaatatgt aatcaacttt ttcaacaatt acatttagtg tttccagatg
       61 cttaaaagtc ggatttgtaa taaaagctat atcataatgt gtatccaaat cgtctctatt
      121 ttgaatatac gttatttctt caaactcttt ttttccactg ctactataag ctgttatatc
      181 aaaattaata gaattctttt ttccatagct tattagattt tttagatgtc tctttccaat
      241 tgaccctaac ccaaaaaaaa ttactttcat cattaattta cctcaatgtc gttaattaag
      301 ttaataatat acttatcttt ttcaaaagta tattggggtt ccattttctt ttccaaaagc
      361 gtgaaaaatt ctttaatctc ctctaaatat gcatcttcaa taatagtatg tgaataatta
      421 ttatcatgca taacctcatc gtacaattga atatctgtca tactagaagt ttcttcattc
      481 catgaaaata gagaatccgg gttaccttcc cattctatta aaatcttttc accaattata
      541 gttaaatttc ttttagctac tcttgaaaca acatctacac ttaaagttcc aactatacca
      601 gtttcatgct caataataag agtgaaactg tccgaataat caatatcaag attggataat
      661 ttctgggtca ttacagtaaa cgatttaact ttcccaaatg tatctataat ccaaggtaat
      721 tcaatagcga atatttctct acacccatta gtttttttgt ttgaaacaaa aaaattgcga
      781 taatcttccc aaggatgcca gtcgggtaaa tactgtccta catggtattg ataagaaatt
      841 tttttattat gcgtcacttt agacttaata tattgaattt cttttctctt caaaaatgta
      901 gaagatagat aaagtaatct atctttcttt ttagctactt cgattatttc atcataatat
      961 tcatttaata aattgatttc cgtaaagata ttacaatcta aatttaaagc ctcttttata
     1021 ataaattcat gggttatagg ggatgtacaa acaaaaactg aatttatttc aaacaaattt
     1081 tttgcctctg ttagactatt ataagttggt attccaaatt ccttttcaac ttcctttcgt
     1141 ctcgcctcat tagtatcaac accacatagt aaaatatcat taaagtgttc ttgaatgagt
     1201 ctaagtcttc ttttccccat ggaacctaaa ccaataattc ctatgttcat aaaatatcac
     1261 acctacttga atattttttt aaactcatca ataacaaatt cttgttcact atctgtcata
     1321 ttacttccag aaggtaagca taagccgcta ttaaataact tttctgacac actaagatta
     1381 ttatcatggt aatacatttt taccccttga aatactggtt gcaaatgtag tggtttccac
     1441 aaagctcttg tttcaatgtt tttttcattt agtttattta ttacgttaac tacttgatca
     1501 atagaattat aatcattgat cgttaaagta gacaaccaac gatttggcac catgccatcc
     1561 acttctggca tcatttcaat tctatcaatt ccagaaaaag cttccttata tcgattgaaa
     1621 atttctcttc gtttagctac tctatcattc aatacttcca tttgtccacg tccgataccg
     1681 gcagaaatat tgcttagacg gtaattataa cctatttcgc tatgttgata atacggtgct
     1741 tgatctcttg cttgtgttgc taaaaatctt gaatgctcaa catatttttt atcactagac
     1801 actagagctc ctccacctga ggttgtgatg atcttattac cgttaaatga atagatccca
     1861 attgtcccaa ataagcctga cgaaattccc ttataagtag aacctaacga ttccgctgca
     1921 tcttctatca ctggaacatt aaattggttt gcaatagcta aaattcgttc ataatcagcc
     1981 atttgaccat ataaattcac tacgatgatt gccttaggta attggttgtt tctttcagct
     2041 tcttctaatg cgctctctag tgcaacaggg ctcatattcc atgtattagg ttctgagtca
     2101 atgaacactg gtacagcccc ttgatataaa ataggattta ctgtagctat aaaagtaaat
     2161 gaaggagcaa agacaatatc gttttttcca acattaagca gtcttaaggc aagatgtatg
     2221 gcagctgtac ctgaacttaa cgcagaagct ccgcccttag aaccagtata ttttgtaata
     2281 ctttcttcaa atttatccac attttcacct aatggcgcta tccaattatc tttaaaagcc
     2341 ttagcaacat atttttcttc tgttcctccc atatgaggac ttgataataa aattctagaa
     2401 ttcaaaatta attccttctt tcttaatttt agctggattg ccataagcta aggaatgtga
     2461 gggaatatcc ttaacaatag tgctccctgc gcctatcata caccaagatc caattttctt
     2521 catctggata cattgagaac caattcctat ttgagttcct acacctacat gtactccacc
     2581 cgctaagcat acacctggag ataaatgaac atagttatta attctacaat catgctcaac
     2641 aatgctacct gtgtttatta tagcatgctc tccaattaca gcatctgggt taattacagc
     2701 attcgcaaaa ataacagttc cataaccaat tgaaatttct tttgccacta tagcacttgg
     2761 atgaacagct attccatatt gctcacttgg catattcaat ttttttataa tctttttt
//
LOCUS       DARBER010000191         2744 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARBER010000191 DARBER010000000
VERSION     DARBER010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.198"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            304..636
                     /locus_tag="SCK06_002700"
     CDS             304..636
                     /locus_tag="SCK06_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152632.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            546..749
                     /locus_tag="SCK06_002701"
     CDS             546..749
                     /locus_tag="SCK06_002701"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1152633.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            933..1088
                     /locus_tag="SCK06_002702"
     CDS             933..1088
                     /locus_tag="SCK06_002702"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1152634.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1364..2704
                     /locus_tag="SCK06_002703"
     CDS             1364..2704
                     /locus_tag="SCK06_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1152635.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          915 a          552 c          519 g          758 t
ORIGIN      
        1 ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc agaactgggc catggtactt
       61 caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta
      121 gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat
      181 tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac
      241 taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact
      301 ctattgaaaa aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag
      361 aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc
      421 cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga
      481 ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa
      541 gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt
      601 acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt
      661 tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag
      721 aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa ccaaattgat gctgtttggg
      781 tgacggatat tacttacatt ccatgtaatg acggtcgcct ctatttgtcc acctatattg
      841 acttagcaac tcgtattcta cgttactata tggtggattc acacatgaaa aaagagattg
      901 tcgtccgtcc attacaaatt tattaaggag aattgcctac ggtaattcat tccgatagag
      961 gtagccagca tcggtctttt atttatcaag agctgttgac cgaccaccac atcacacatt
     1021 caatgagcca aactgggact cctgttgaca atgcagtaat tgaagcgttt catcgatcaa
     1081 tcaaatgaga attaatcgac tccaacaaat ataaaagtaa aatagagatg aaggtgttaa
     1141 tccaagatta tttagaagac tactacccaa acaaaagaat ccatacaaaa tttatgatga
     1201 ctccttgaca atttgaagcg aagaaaataa gggatctata atatctagtg ttggtgacgc
     1261 cttctgttgc caatactttt tcttttttct aataaacgca aacagacacc ttgaaatcct
     1321 ttagaataaa gtcgacgaaa accattcaaa ggagtgtttc aagatgtcct attcagagtt
     1381 taccaaagag ttactagata ttctagacct aaatcttacc tttcatgaag atgcctttcg
     1441 caaagagcgc attaatgacg aaacatgttt tgtttttgat ggcacattga cctatcaacc
     1501 agaggaatgc ttccattgcc actatcaaaa taagcaaacg atcattaaat ggggctggaa
     1561 aaaagtatcc atcttattga atgatgtcag taactacaag accattgtgc ggattaacaa
     1621 acagcgcttc aaatgtaaac actgtggaaa gactttttta gcagaagatt cagttagcga
     1681 tcgtcgctgc tccattgccc gcagagtcaa acaagccatt cttgagctat taagtgagcc
     1741 tatttctatg tctttgattg cacgtatgaa gcacatttct ccgaccactg tcattcgaat
     1801 acttcgcagc cttcggccta aaaccgtttc tttgaaccag ccgttaccag aagtggtgtg
     1861 tttcgatgaa ttcaaatcgg tgaaaaacgt ctcgggagcc atgagctttg tcatgatgga
     1921 cggcaagacc catcaactga ttgatattgt ggaaaatcgg caactcaatc ctttacgcga
     1981 ctatttcttg cgctacccta gaaaagtccg tgaacaagta cgtctagtgg tttcagattt
     2041 ctatacaccc tatcgcacat tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga
     2101 tcgctttcat attagccaac acatcggtcg ggcctttacc aaccaccgaa ttcaagtaat
     2161 gaagaccttt aaaaaagggg atcgtcggtc gaagcactta aagaaatact ggagactact
     2221 tcaaaagaat gcgtgggaat tgaatggtca gcatcgctac tggcgtccgt catttcgaga
     2281 tcatctgaca gaagcggaga tagtcgatcg gctattatat tatgatgact ccttaaagcg
     2341 aggctacgaa gtctaccagg tttttctttc cgctatcaga agacaagatg ttcctgaatt
     2401 cgtcgcgctc ctaaaagaag actacaagga gttgccagag cactatcaac cagtgttcac
     2461 gaccttcaag aaatatcgaa cagaaatcaa acgagcgttg cgagttccct attccaacgg
     2521 ccctatagaa tgtttgaaca accatatcaa agtactaaaa cgaattgcct atggctttag
     2581 aaactttcaa aactaccgag aacgaatctt tttatatcga ggaaaatatt tcaagaaaac
     2641 taagaacact acccaactga ctaaagccag aacaacaaca cggctagaca agatagctgt
     2701 ctagccgtgt gagtatctcg attattctat tgaatttctt cacc
//
LOCUS       DARBER010000192         2737 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARBER010000192 DARBER010000000
VERSION     DARBER010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.199"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            174..518
                     /locus_tag="SCK06_002704"
     CDS             174..518
                     /locus_tag="SCK06_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG1152636.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="SCK06_002705"
     CDS             519..938
                     /locus_tag="SCK06_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG1152637.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="SCK06_002706"
     CDS             complement(1030..1380)
                     /locus_tag="SCK06_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152638.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="SCK06_002707"
     CDS             complement(1346..2566)
                     /locus_tag="SCK06_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG1152639.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1015 a          493 c          528 g          701 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactat
//
LOCUS       DARBER010000193         2717 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARBER010000193 DARBER010000000
VERSION     DARBER010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.200"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            391..>2717
                     /gene="esp"
                     /locus_tag="SCK06_002708"
     CDS             391..>2717
                     /gene="esp"
                     /locus_tag="SCK06_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG1152640.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          982 a          419 c          537 g          779 t
ORIGIN      
        1 aaggaatttg aagtcacctt tttacttatg tcacagtctc attcagtaat gcgggataag
       61 gctccttatt ccgttatttg aatactctga agtttaatct aagaaagggt aatattttga
      121 aaacatgctt catcgcatag aaatgaactt aatttcagaa agaatacata catatctttt
      181 tatatgtttt cagctgctga atggttaaaa agcacaaata tccgaaaaaa acatgaagaa
      241 atgtactttt tttgttgtaa tgttcatgaa attctctaag taaattagtt aacataaggt
      301 tagtaagatt cctattctgt gaataatacg tatcagattt atcttttatt cttactagga
      361 aagtaattat taagggagtt gatttgaata atggttagca agaataataa gagagtattt
      421 ctagaaaaaa caaagaaacg agtattgaag tacagtatca aaaaattaag tgttggggtt
      481 gcatctgttt tgattggtgt tggtcttgtc ttgggaacaa ctgaactagt taaagcacaa
      541 gatgaaattt ctccaagcag cccacttgaa acagcgataa gctccgtaca agtaggtgac
      601 aaagtagctt cggggaatac ttttcaggag aatcctggtt atacgaaaaa ttataacttt
      661 tcggatttac aatttagtcc tcaagaacta actggagaca cactaaaagg caatacaatt
      721 ggatttgaag tttacggaaa acataatatt gcagcatcaa ctaaaaactg ggaaatccgt
      781 cttcaattag atgaacgatt agctaaatat gtagaaaaaa ttcaggtcga tccaaagaaa
      841 ggaattggat cttctagacg gacctttgta agaattaatg attcgctggg tagacctaca
      901 aatatttgga aggtcaatta tattcgtgca agtgatggat tatttgctgg ggcagaaaca
      961 accgatacgc aaaccgctcc taatggggta attacgtttg aaaagagcct agatgaaatt
     1021 tttaaagaga taggcataga caatctcaag accgatcgtt taatgtatcg aatctacttg
     1081 gtaagtcatc aagacgatga taaaattgta ccgggtattg atagtacggg ctacttttta
     1141 acagattcag atgatttcta taatagctta gatgtatctg aaaataatcc tgaccagttt
     1201 aaacatggat cagtaagtgc aaaatatgag aaacctaata ctcaaacaaa agatggttca
     1261 ggatccacag gtgctaatgg agctataatc ttagatcata agttaacgaa aaattataat
     1321 ttctcatatt catcttccgc aaagggcacg ccttggtatg ctaattacaa aattgatgaa
     1381 cgattagttc catatgtagc gggtatacag atgcacatgg ttcaggctga caaagtaaca
     1441 tatgatgttt cgtttgaatc cggaacaaaa gtagctgatt tagcaatcga gcgacgggaa
     1501 ggtcatgaaa attatggcat aggttcaata actaataatg atttaactaa acttatcgat
     1561 tttgctaatg caagtccacg tccagtcgtt attcgatatg ttttacaatt gactaagcca
     1621 ttagatgaaa tcttagaaga tatgaaagca acagcacaag ttgaagaaaa taaaccattt
     1681 ggtgaagatt tcatctttga ttcttggttg tcggatacga ataaaaaatt aatccagaac
     1741 acttatggaa caggttatta ttatttgcaa gatattgatg gtgatggaaa ccctgacgat
     1801 aaagaagaga gcggagacac gaatccatat atcgggaaac ctgaattaga agaagtatat
     1861 gatgttgaca caacagttaa ggggaaagta ttcatccacg agttagcggg aacaggtcac
     1921 aaagcccaac ttgttgataa agaaggtact gtattagcag aaaaaactat cgctccaaat
     1981 gaaaaagatg gggctccaat ttcagatact gtagaatttg aatttacggg tgtagattca
     2041 agtaaattaa tcgcgaaaga tgaattaaaa atccaaatcg tttctccagg ttttgataaa
     2101 ccagaagaag gttcaaccgt tattaaggaa tcaccaaaag cggttgataa acaaaccgtg
     2161 gtagttggat ttaaaccaga tgctaaagaa tcaattcgga ataataaaaa cttacctgaa
     2221 gatgcagagt attcatggaa aacagagcct gatacttcta acgttactga tagtacgaaa
     2281 ggtattgtaa ctgttaagat cggaaatcga actttcgacg tggatgtaga gtttgctgta
     2341 aaagcttctc aagctatgga aaatgatgca acatacgtac ctataacaac aactccagaa
     2401 acgacagttc aaagtggaaa acctacattt gataaaccag atgttccttt agctaaagat
     2461 gccttttcaa tttcagatgt ctataataag tacttcggta atgcaagtgt tgatgcaaac
     2521 actggtattg ttacattcac tccagctaaa ggtgtaggag aatcggagcc gattactgga
     2581 acaattccta ttaaaattgt ttaccaagat ggttctgtag gcacgaccga tttagcagta
     2641 actgtaagta aagatattta tgaaaatcca ggagaaaaca ttcctgcagg ctaccacaaa
     2701 gtaaccttca ccgcagg
//
LOCUS       DARBER010000194         2579 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARBER010000194 DARBER010000000
VERSION     DARBER010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.201"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            7..1014
                     /locus_tag="SCK06_002709"
     CDS             7..1014
                     /locus_tag="SCK06_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1152641.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1026..2030
                     /gene="neuB"
                     /locus_tag="SCK06_002710"
     CDS             1026..2030
                     /gene="neuB"
                     /locus_tag="SCK06_002710"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEG1152642.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2027..>2579
                     /gene="neuC"
                     /locus_tag="SCK06_002711"
     CDS             2027..>2579
                     /gene="neuC"
                     /locus_tag="SCK06_002711"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEG1152643.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRL"
BASE COUNT          944 a          333 c          513 g          789 t
ORIGIN      
        1 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
       61 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
      121 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
      181 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
      241 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
      301 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
      361 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
      421 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
      481 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
      541 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
      601 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
      661 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
      721 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
      781 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
      841 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
      901 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
      961 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     1021 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     1081 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     1141 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     1201 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     1261 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     1321 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     1381 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     1441 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     1501 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
     1561 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
     1621 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
     1681 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
     1741 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
     1801 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
     1861 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
     1921 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
     1981 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
     2041 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
     2101 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
     2161 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
     2221 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
     2281 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
     2341 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
     2401 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
     2461 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
     2521 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattac
//
LOCUS       DARBER010000195         2522 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARBER010000195 DARBER010000000
VERSION     DARBER010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.202"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            256..531
                     /locus_tag="SCK06_002712"
     CDS             256..531
                     /locus_tag="SCK06_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152644.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..>2522
                     /locus_tag="SCK06_002713"
     CDS             544..>2522
                     /locus_tag="SCK06_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG1152645.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRN"
BASE COUNT          759 a          394 c          553 g          816 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 at
//
LOCUS       DARBER010000196         2505 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DARBER010000196 DARBER010000000
VERSION     DARBER010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.203"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..435
                     /locus_tag="SCK06_002714"
     CDS             <1..435
                     /locus_tag="SCK06_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1152646.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="SCK06_002715"
     tRNA            complement(759..842)
                     /locus_tag="SCK06_002715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="SCK06_002716"
     CDS             1370..1870
                     /locus_tag="SCK06_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1152647.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="SCK06_002717"
     CDS             complement(2060..2404)
                     /locus_tag="SCK06_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG1152648.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          785 a          478 c          413 g          829 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaa
//
LOCUS       DARBER010000197         2478 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DARBER010000197 DARBER010000000
VERSION     DARBER010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.204"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(277..2361)
                     /gene="cadA"
                     /locus_tag="SCK06_002718"
     CDS             complement(277..2361)
                     /gene="cadA"
                     /locus_tag="SCK06_002718"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1152649.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          724 a          539 c          373 g          842 t
ORIGIN      
        1 atgataaaaa tttgttatcc agatgtaatc atattcaatt tttaacaaac ttacaagaca
       61 gtgttatttt atgtgtgtat tcttttattt aatacacatc gttttttcat attttttcca
      121 tcaaaaaaat aagttatttt cttttatttc attgacctta ttgaatttca agctattttg
      181 aattttctgc aaaattaaaa acattagtta ttttataatc ctatctattt tctttacatt
      241 tttcattaag aaaaggatat aaaaaaaaga ggacgttcat tcgtcctctt tttttacatg
      301 caagcttcgc atggcattca aaacagctaa tactgtcacc ccaacatctg caaatactgc
      361 atctcccatt gatgcaaaac caaaagcacc taaggcaagt accaaaattt ttatcccaat
      421 agcaaagaca atgttttgtt tgacgatttt caatgttttc tttgctaaat ggattgcttc
      481 tgcaatcttc gatggttgat cattcatgat gactacatct gctgcttcaa ttgcagcatc
      541 gcttcccaat ccacccattg cgattcctac atctgctctt gctagcaccg gtgcatcgtt
      601 gattccatct ccgacaaatg cggtcttttt attgtttctt tgtaagattt cctctaaccg
      661 ctgcacttta tcttcaggaa gcaattcgct atatatcttg tccacgccta cttgcttccc
      721 gatatgatca gcaatttttt tcgagtcgcc cgtcaacata actgtgttct tcacaccagc
      781 ctgttttaat tgaaccaatg cgtctacggc atcttctttc aaggtatctg caataacaag
      841 ataaccactg taactttgat cgatcgctag ataaagcaat gtaccgattt cttgacttaa
      901 aggaaaagag atttcaaatc tttccatcaa tttgtgatta cctgctaata cttgtttgcc
      961 atcaacttca gctaagatcc cttcacctgc aaattcctgg atattagttg ttggtaaaag
     1021 cggacctgcg tagccttcta aaacagattg agcaatcgga tgtgtagaaa actgttctgc
     1081 actcgcaata tattgcatga atttctttgg atcgattttt gtatcgatcg tctggatcga
     1141 aaagtcacct ttcgttaatg tgccggtttt atcgaatacc actgtctcgg tttgcgcaag
     1201 caactccaag taatttgaac cttttactaa tacaccgatt ttacttgcgc ctccgatacc
     1261 gccaaagaaa ctaagtggca cggaaatcac taatgcacat ggacatgaga tcactaaaaa
     1321 tgtcaatgct cgatagatcc attcagaaaa cgccgctcca gaaacaacaa gtggaggtaa
     1381 aacagctaat aaaacggcca atcctacaac gactggtgtg taatagcgcg cgaatttagt
     1441 aatgaaattt tcagctggtg cttttttact gctggcattt tctactaatt ctaataattt
     1501 gctgatagta gaattttcat aagatttttc tacttgaact aaaaatggtt tatttttatt
     1561 gataaatccg cttaatactg tttcaccaac atatacagat ttagggactg attctcctgt
     1621 aagtgctgac gtatcgacta atgattctcc ttcgatgatc gttccatcta acggtacacg
     1681 ttcaccaggc ttcacaagta caaactgacc gatctgcacg tcttcagggt tcacggtcat
     1741 caatccttcc gtcgtctgca cgtgagctgt ttctggacgg atcgccatca attcgcgaat
     1801 cgattttctt gaatgactga ctgccaatcc ttggaaatac tcgccgattt gataaaatag
     1861 catcactgct acggcttctg gatattcacc gatcaacata gcgccaatcg tagcaacaga
     1921 cataagaaaa ttctcatcaa agatttttcc tttgacgata ttcatcactg ccttcttcac
     1981 tacatcatat cctatcagca gataaatcaa taaataccct atcaagcgaa ttggttcatt
     2041 aaaatcaaat aatagcaata aagcgatacc tgcaagggaa aataaaatcc ggaacagcgt
     2101 atttctctct ttttcatttc catgttcaga tactttttcc ttatctattt ctgttacttt
     2161 gacatctggt tctaattcct tcactacttt ttttgtttct tgttcaatcg tagccaaatg
     2221 atcttcctct gcgtcaatcg ttagttttcc agaagtgaag tttaccgttg cttccttgac
     2281 tccattgatc ttctgaactc ctttttcgat tttcattgca cagttcgcac aatctaaacc
     2341 ttctaatctg taacttttca ttttaatgac ctcctcgcct caacatatga acatctcttc
     2401 ataactaatt ataaatgaat ggttattcat atgtcaaggt atccgaaaga aaaaatgaaa
     2461 aacatctgca tcggaaaa
//
LOCUS       DARBER010000198         2464 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARBER010000198 DARBER010000000
VERSION     DARBER010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.205"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            560..2053
                     /locus_tag="SCK06_002719"
     CDS             560..2053
                     /locus_tag="SCK06_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG1152650.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2188..>2464
                     /locus_tag="SCK06_002720"
     CDS             2188..>2464
                     /locus_tag="SCK06_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG1152651.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRK"
BASE COUNT          852 a          379 c          491 g          742 t
ORIGIN      
        1 atatattcga tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata
       61 tagtggatat actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa
      121 aatatatttg tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat
      181 agaaaccaca gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag
      241 aaaaattaca taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg
      301 aatcacatgg ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac
      361 tacttatatt aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag
      421 gcgagcaact caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac
      481 tgaagagaga acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg
      541 agaaaggacg ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt
      601 acgtacatac aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag
      661 gagtggtgct atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt
      721 cattacttcg gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa
      781 tacctatcgc tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga
      841 gaaaaactta agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca
      901 aggcgaaatt attttagcct gtttagatca agtgggctac atgccaacgc taattttaga
      961 atcagatcat ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa
     1021 aagcaatttc aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt
     1081 ggctgaaaaa ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc
     1141 acaagaaaat aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat
     1201 tcaatggtcg atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact
     1261 aattgtcttt cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt
     1321 acttcataaa gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt
     1381 cacgctttct ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat
     1441 gttcacattc aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt
     1501 acgtagtgct tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg
     1561 ccagcaatgg gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg
     1621 gtggaaattt aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga
     1681 agatctattg gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc
     1741 aaaaaaagaa ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa
     1801 tgagttacga gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat
     1861 gatcattgct tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag
     1921 actagattat tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga
     1981 gacatttaga caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt
     2041 agatacaggt tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc
     2101 tgtaggtgtt gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc
     2161 agtcttatgt atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct
     2221 gttaatgtgt ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat
     2281 ggttttaagc aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg
     2341 ccgattgagt ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat
     2401 gattgtttgt gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga
     2461 aaaa
//
LOCUS       DARBER010000199         2442 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DARBER010000199 DARBER010000000
VERSION     DARBER010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.206"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..2291
                     /locus_tag="SCK06_002721"
     CDS             138..2291
                     /locus_tag="SCK06_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG1152652.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          833 a          427 c          542 g          640 t
ORIGIN      
        1 gtgattgctg tgaaacaaaa agttgatttt atcttgaaat taggtaaaga atttcttgct
       61 ccgcccattt tttctttttt attaagaagt ttttgggtac aatgaagcat gaatgtaaat
      121 aggcggaagg ataggaaatg atgaaagatt ttatgaaaaa aatattaaat acttcttggt
      181 tagaaaaaat aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg
      241 ttccttttcg cttaaatttt ctttttttca ttatatttac gttatttgtt gcgttgattg
      301 ctagattggg ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta
      361 cttctacgat tacggtccaa gaaagtactc ctcgcggaat gatctacgac tccacaggaa
      421 aagctttagt tacgaataaa gcgaatcagg ccatcacata tacgagagga acacaagtta
      481 cggctaaaga tttgcttgaa atcgcaacga agctgaactc gctgatcgat gttccggtag
      541 atgaaaatct gacagataga gataaaaaag atttttggct ggctgatccc aaacatttga
      601 aagaagccac aaaaagactt tcggcaaaag aaaagcaatt agggaacagc gaacaatata
      661 gtgcgatggt caataaagtc acagctgatg agattaattt aaacgaggag caattaaaaa
      721 ttgctaccat ctttaaacgg atgaatagtg catatgaatt aaacactgtc tatatcaaaa
      781 actctggtgt tactgatcaa gagttagctg ttgtggcaga acgcgcttct gaacttccag
      841 gagtttcgac tggcacagac tggacacgag aatataatgc agcaagttca ctgaaaagta
      901 ttcttggcag tgtgacgact gaaaagcaag gacttccagc agatgaagca gagaaatacc
      961 tagcaaaagg ttattctcgg aatgaccgtg taggacaaag ttatttggaa aaacaatatg
     1021 aagatgtcct gcaaggaacc aaaacccaat atgaagtttc ccttgataat gaaggaaatg
     1081 tttcgaatca aaaagaaatc ttctctgggg aaaaaggttc taatttaatg ttatctatga
     1141 atgcagaatt ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa acattgatca
     1201 acaacggcaa agcacagtat tcgcctggtg cttatgcagt ggctatgaat cctcagacag
     1261 gagaagtgct agcaatgact ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga
     1321 atgctttagg cacgattact agcgcctttg atccagggtc tgtagtcaag gctggtactt
     1381 tgacagcagg ctgggcaagt aacgcgatca gcggaaatca agttttgatt gatgagccga
     1441 ttcgtctgca aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc
     1501 ttgatgcagt caaagcatcg gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa
     1561 agatgttagg actagactat acacctggaa tgggactacc tagtttagat gaagaagcta
     1621 aagcttatca gcaattgaga gactctttca aagagtttgg tcttggtaca acgactggga
     1681 tcgatttgcc gaatgaatct cctgggatct cacgttctgt tgattatatg aagaagttca
     1741 atgcggataa tggcaaagaa tggtatgctc caggtaactt taccgatttg gcatttggac
     1801 agtttgacac atatacacca atccaattag ctcaatatgc ttcaacagtc gctaatggcg
     1861 ggaaacgagt acagccgcac ctagtcaaag cgatctatgg caatgacgaa aatggaaact
     1921 tgggtgaagt gaagaaagaa attggcacaa cagtagaaaa tacggtcaat atttctgcag
     1981 aaaatatggc gatcctccgt gaaggattcc accaagttgt ccatggtacc gatccttaca
     2041 caacagccaa accattagcc agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag
     2101 aaacggttgc ggaaggtcat ccagatattg cgacagtcaa tagtaatatc gtggcatacg
     2161 gaccaacaga caatccggaa attgccatca gtgttgtctt gccgaactta ttagacgaac
     2221 aagaccacat aaacttgacg attgctaaag aaataatgga cgcgtattat gatatgttca
     2281 tggcaaaata aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt
     2341 tgtgaaaata gcttctcaca tttttgctta aatttgactt cataccgaga aactggcttt
     2401 tgaacaccgt ttactcggca aaaaaggttg tgacaaaaat tg
//
LOCUS       DARBER010000200         2420 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARBER010000200 DARBER010000000
VERSION     DARBER010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.207"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..213
                     /locus_tag="SCK06_002722"
     CDS             <1..213
                     /locus_tag="SCK06_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEG1152653.1"
                     /translation="MLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAKQA
                     TFQLNDLNKAFLASHIYGKILSAKFE"
     gene            232..>2420
                     /locus_tag="SCK06_002723"
     CDS             232..>2420
                     /locus_tag="SCK06_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1152654.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHL"
BASE COUNT          883 a          325 c          447 g          765 t
ORIGIN      
        1 atgttgtatc gtgaattact ttcagatgaa gaatcaaaag aagatctgat tgcaacaatc
       61 gttgatattt tggggtatgc gatttcgcca gagtatcttt tcaacgtctt agcggatcaa
      121 gcaaaacaag caacgttcca attgaatgat ctaaacaaag cctttttagc aagtcatatt
      181 tatggaaaaa tactgtcggc aaaattcgaa tagtgtcgag gaggtgctgt gatggaagaa
      241 ttaaaagaac gcattgtcaa ttatgtcata gagaactctg agattaaaaa taaatggggg
      301 aacaatacag cacagaaaat atctgaaaaa ttaaatgtta gtcgtaatat tgtctctcaa
      361 tatttgaatg aattcgtaaa agatggagaa tttggaaaaa tcaatactcg tccagtaatc
      421 ttcttttctt taccggagaa agttccaaat caacaaattt ttagctcttc agaggaagcg
      481 gaaaaatggt atgatagctc ttttagggtt ggaatagaaa gggttatcgg aagtcaagga
      541 agtctgcgaa atgtaattgc tcaaattaaa gcagcaatct actatccgcc aaatggctta
      601 cccattttac ttcatggcga gactggaact ggaaaaagct atttagcaaa ttatgtgtat
      661 aaggatttgt tggccaataa ttatatagaa gagaaggcat ctttcatttc agttaattgt
      721 agtgaatatg ccaataatcc agaacttttc ttgtccaatt tttttgggca tgtaaaaggt
      781 gcatatacag gagccgaaga tggaagaaag ggtttagcag aattagctaa taatggagtc
      841 ttatttttag atgaaattca tgcattgagc aatgaatgtc aggagaaact ttttcagttt
      901 atggatacga ggaaatttca tcggatgggt gataatgaaa actggtacac atcaagttgc
      961 tggattatat ttgcaacatc agaaccacct gaggataaac tattatcaac tttgttgaga
     1021 agaatacctg taaagctaac acttccaaca ttagacagtc gttctcttta tgaaagaaaa
     1081 cagttaatca agatattttt taaatcagaa gaagaaagac tagcaaagaa aatactgata
     1141 tctcaacaat tattagattt tttattaaac tatcgcttta gtgaaaatgt tggcaatttg
     1201 aaaaatatca ttcaattgac ctgtgccaat gccttttatt tcttagatga tttggaagaa
     1261 ctttatattc agatgtatca tttaccacaa tgggtgaatt tcaacggcat gaaggttatg
     1321 aaaagacaga atgaattaaa atatattcaa atggatgatt tggaaaaagt agacgaagtt
     1381 tttaatatca acgaacttat agagtatctt tatcaaacgt ggaagaaaga cactgataag
     1441 cagcagcttt ttgagaaata tgatagatat tttagacaaa agaatgttga tatccaaaat
     1501 aattcagctt taacatatcc cacctatcaa gagattcgat ttagacagta tgttaaggaa
     1561 atagagagag attttcaaat agaatttgac tattattatc ttcaaataac gtctatcttt
     1621 ttgaattctt ggatttatca aatccaatta acatcggacg ataaattata ttccttcatc
     1681 aatgatatta atagttggtg tagtcgagaa gttcaattag tcaatcgttt tattcaaata
     1741 ggggatattt atttaacaaa aattcaaaaa aatgttttgg ttattttatt taagttaatg
     1801 tgtagtaaag aatttcagca aaaacgttta gcactgattt tggctcatgg agatctaacg
     1861 gcaacctgta tagcaagtac tgctaatagg atcgttgaga gatatatata cgacgccatt
     1921 gatatgccac taatgacttc aagtagtgag attgcagaca aaattaatag atacttacaa
     1981 gagtgtaccg gatatgaaga aattatttta cttgttgata tgggttcact ggagaaaatt
     2041 tatctaaatt tggaactaga cgctcaaaaa aaattcggac tactaaacaa tgtttcaaca
     2101 agaatggcta ttgatgttgc aaatcagata ttattagatt taccgcttaa acagattctt
     2161 gaacaagtaa aagaaaatca aaatgtggat tatatttatg aatcatcaaa tattaagaag
     2221 gatatgattg tctgttcttg tgcaagcgga ttaggaactt cgtttaaatt aaaaaagata
     2281 cttgaagata gctttccaag ggatactgat cttgtcatag aaacgtgtga ctattactct
     2341 ttagtgaatg aaaactattt atcggagctt cgtaaagaga acaatattct ttttattgtt
     2401 ggaacactaa atccacatct
//
LOCUS       DARBER010000201         2409 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DARBER010000201 DARBER010000000
VERSION     DARBER010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.208"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(244..465)
                     /locus_tag="SCK06_002724"
     CDS             complement(244..465)
                     /locus_tag="SCK06_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152655.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(563..808)
                     /locus_tag="SCK06_002725"
     CDS             complement(563..808)
                     /locus_tag="SCK06_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152656.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1048..1662
                     /locus_tag="SCK06_002726"
                     /pseudo
     CDS             1048..1662
                     /locus_tag="SCK06_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2032..>2409)
                     /locus_tag="SCK06_002727"
     CDS             complement(2032..>2409)
                     /locus_tag="SCK06_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1152657.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          842 t
ORIGIN      
        1 tactatatac ttcttcccag aacttgtccg tctccttcaa attatctcta cctctcgagt
       61 tcattgggtg gtttgcccat ttattaaaca tggaatcacc tttctcatac tcatatattt
      121 atgtcctatt tcaattatat ttatatatgc ttaaataaat ttgtatcctg aaatttattg
      181 taatatgaag ctgtatttta catatagtaa ccaaagagtt aataaaataa aaagagcgaa
      241 ccattataga tcagctcttt tttcattgct gtttttaatc ttatctagaa tcacttcaaa
      301 tgtttcaaag gtatttacat ctaactgaca taaccaaagc aagtacttct tttctttgta
      361 agtccaatca atattacggt aagaggacat tagattatgg taccttgctc tttgttcaac
      421 tgaagccaat tcccagcgtt tttcaaatat tttttcttct tccattattg atccctcgca
      481 agacgctacc tcaaacttaa ataatgggca gcgaaagtct aattccttag atctaagtat
      541 agcaaacaaa tatgaaaaaa acttagaagg tagtaaaagt tcttccgtct tttgctttgc
      601 ttctattctt aatgtgtttc gttatcggtg ccctacaaag attttcatta attacacact
      661 catttattgt tgtgtatttt ctgacatttc tctcttgatc aattatttta attccatggg
      721 catcctgata gttcatagga gaggagcggc tatcagaaat agttgcacct atctcttgaa
      781 cttcttttac aacttctgga aaatccatcc atcctgcttc agctgtcaaa agtaataatc
      841 tataatattt atctttcctt ttcaatctaa gaaatcgtca ccccattctt tttcttctat
      901 tataaactat ttaactttta gaattcaaaa accgtttgtc tatttagagt ttaccctaaa
      961 tagacaaaaa tataacattg aaaaatttag agttctaacg tatgattagg gtgtattata
     1021 aacgttatag aaaagtaggg aataaatttg aaaattgctt atattagagt ttcatccatc
     1081 gatcaaaatg aacaacgtca aattgaagaa atgaaaaagt ttggcgctga aaaaattttt
     1141 atcgaaaaac aatctggagc gacgattagt catcggccga ttttccagga agctttagat
     1201 tttgtcagag agcaagatat ttttattgta gaagccattg atcgacttgg acgcaattat
     1261 gacgagatta tcgattcagt gaattatcta aagaagaaga atgtgcgact tattatcacc
     1321 agtttaccga tcatggctga agcaattgga aatcctctcc tagataaatt catcaaagac
     1381 ttaatcattt aaatctcagc catgattgct gaacaagaac gtactgaatc gaaacgacgc
     1441 caggcacaag gaattaaaat cgctaaagca aacggcgtct ataaaggtcg tcctaagtta
     1501 tacagtgcag acaccaaaga tcctcaacgt cgccttgtct ataggagtat cgttcaggat
     1561 cttgagaatg gggttgctat ctcaaagatt gcaacagact acaatgtgac taggcaaaca
     1621 atttaccgaa taaaaaaaga aatcgaccaa ctaattgtat gatttctttt ttgaatcacg
     1681 taattattaa ttagctttgt catcaataca gacaatcatt ttagattcat ctagctaagt
     1741 tgattacatt tatgttaaaa agaatttagt taacataaat ataattgatg cataaatcgt
     1801 ccttatttaa aggcatttaa cattgacaac ttagtataat gtatcttatg taaactaata
     1861 agatttgaat tttattttat acacactcga taatagatca attttatggt cccccgcatt
     1921 tacgattgca caagagaaac ataaagtcga cctattagca tttcttttca attcaaacat
     1981 taaaaaagtt tatacctctg ctactaaaaa atgaaattat ctatttaaaa tttaaaacgg
     2041 taaatctgat tcatccaaat tggaaaatgg atcagcctgt attggagctt tgtatttctc
     2101 cataataaca gatatctttt tttcattttt taaaaaatac cttttaaatt tcttatagtc
     2161 ctctgaaatc aaaatatagc gtgatggata ctcatcaaaa gaagaatcgt attcttcctt
     2221 taaaattccc gatttactta atttttctag tacatcaaat actctatcac tcaagtaact
     2281 ccttaaattg ttatcacttt cccattgaac tacttttcta taagttgaat tatcagttga
     2341 aaagatagaa ttttcatcca taaacgcata tattagcagc aatctttcat cattgtcaaa
     2401 ttctattgg
//
LOCUS       DARBER010000202         2407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DARBER010000202 DARBER010000000
VERSION     DARBER010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.209"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SCK06_002728"
     CDS             5..1150
                     /locus_tag="SCK06_002728"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG1152658.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SCK06_002729"
     CDS             1321..1779
                     /locus_tag="SCK06_002729"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG1152659.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SCK06_002730"
     CDS             1938..>2407
                     /locus_tag="SCK06_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG1152660.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARBER010000203         2398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARBER010000203 DARBER010000000
VERSION     DARBER010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.210"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            207..2069
                     /locus_tag="SCK06_002731"
     CDS             207..2069
                     /locus_tag="SCK06_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1152661.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="SCK06_002732"
     CDS             2069..>2398
                     /locus_tag="SCK06_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1152662.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DARBER010000204         2261 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARBER010000204 DARBER010000000
VERSION     DARBER010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.211"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            278..820
                     /locus_tag="SCK06_002733"
     CDS             278..820
                     /locus_tag="SCK06_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152663.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="SCK06_002734"
     CDS             976..1425
                     /locus_tag="SCK06_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG1152664.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2261)
                     /locus_tag="SCK06_002735"
     CDS             complement(1455..>2261)
                     /locus_tag="SCK06_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG1152665.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          784 a          372 c          429 g          676 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgtttcatg
     2101 attcgaccga ttcggcgccg actgagttga agcccacgct ttgctaaaca cttcttcaat
     2161 tttcgggtac cgtaagcctt tcggtttcga ataaactctt cctgaacgat ctcttctaag
     2221 tcggactcat tttcgatcgg tttcgcttga taatagtagg t
//
LOCUS       DARBER010000205         2243 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DARBER010000205 DARBER010000000
VERSION     DARBER010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.212"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..385
                     /locus_tag="SCK06_002736"
     CDS             <1..385
                     /locus_tag="SCK06_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152666.1"
                     /translation="HYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRG
                     TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKR
                     IAFGYRNFYNFRARIYLQQGLIFEN"
     gene            440..2005
                     /gene="guaA"
                     /locus_tag="SCK06_002737"
     CDS             440..2005
                     /gene="guaA"
                     /locus_tag="SCK06_002737"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1152667.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          693 a          459 c          457 g          634 t
ORIGIN      
        1 gcattatcat cgactgttta aacgaccaat tagtcaaaca gaaatcgttg atgagctgtt
       61 aagctacaac gaagaattgc gaactgcgta tcatttttgt caattattac gctattattt
      121 cgttaaacga ggtacggaag gctttcaaga aacccttaaa acaatttcat ctacgttacc
      181 tcagtcgttt aaaaagaaat tcacgatctt ccatcgctac caatcgggta tttcaaatgc
      241 ctttaaggtg tcccactcaa acggcgtcac tgaggggctg aataacaaga tcaagctgat
      301 taaacgaatt gcgtttggtt atcgcaactt ttataacttt cgggcacgga tctatttaca
      361 acaaggtctg atctttgaaa actaaaaaaa tatgcggcgc acagaacgcg ccgcatatcg
      421 gactaagttt tattcaaatt tgtcttcacc aacacgattt gacgaagagc caaaaattat
      481 cgtacttgat tacggtagtc agtataacca actgatcact cgccgtattc gtgagttcgg
      541 tgttttctca gagttgatga gccaccgcat taccgcagaa gaagtaaagg caatgaaccc
      601 taaaggaatc atcctttctg gcggaccaaa cagtgtttat gatgagaagg cctttggtat
      661 tgatgaaaag atttttgatt tggggatccc agttttagga atctgttacg gtatgcagtt
      721 gattacttat aaattaggcg gaaaagttaa accagccaaa aaccgtgaat acggaaaagc
      781 agaattggaa atcacttctg cagaagcggc ccttttccaa aacactcctg aaaaacaaac
      841 cgtttggatg agtcatggtg acctagttac ccaagcaccg actgatttcg atgtcgttgc
      901 taccagcaaa gattgcccca tcgcatcgat ccaaaatgca gaacgcaaaa tgtacggaat
      961 ccaattccat gcagaagttc gccattccga atatggaaat gacttgttgc gccattttac
     1021 actagatgtc tgccaatgta ctggtgactg gacaatggat aacttcatcg acatggagat
     1081 cgcaaaaatt cgtgaacaag tcggtgacaa aaaagtttta ttaggccttt caggcggagt
     1141 agattcctct gttgttggtg ttcttttaca aaaagcgatc ggcgatcaat taacatgtat
     1201 cttcgttgat cacgtattgt tgcgtaaagg tgaagcagaa caagttatgg aaagtcttgg
     1261 cggaaaattt ggcttgaaca ttataaaagt cgatgccaaa gaacgtttct tatcaaaact
     1321 tgctggcgtg tctgatccag aagaaaaacg taaaatcatt ggaaatgaat ttgtatatgt
     1381 cttcgatgat gaagcaacca aacttgccgg tgaagaaggc gtttctttcc ttgcacaagg
     1441 aaccctttat accgatgtga tcgaatcagg aactgaaact gcccaaacca tcaaatcaca
     1501 tcacaatgta ggtggattac ctgaagacat gcaattcaag ctgatcgagc cattgaacac
     1561 attgttcaaa gacgaagtac gtgaattagg tacccaatta ggcatgcctg acagcatcgt
     1621 ttggcgccaa ccattccctg gaccaggctt aggtattcgt gtccttggtg aattgacaga
     1681 agaaaaatta gaaatcgtcc gtgaatcaga cgctatcttg cgcgaagaaa tcgccaatgc
     1741 cggtttagat cgcgacatct ggcaatactt caccgtccta ccaggcattc gctctgttgg
     1801 ggttatggga gatggccgta cgtatgacta cactgtaggt atccgtgctg tcacttctat
     1861 tgacggcatg accgctgact ttgcccgtat cccttgggaa gtcttgcaaa aaatcagtgt
     1921 ccgtatcgta aatgaagtcg cacacgttaa ccgtatcgtg tatgatatta ccagcaaacc
     1981 acctgcaaca gtggagtggg aataaagagc caacataata tggatgagga atacctataa
     2041 atcaacatta ttcaattact aaagttcctg attttcgtaa aaaataagat ggtttcctac
     2101 cattttccct accaaagccc ttgttagatt aactactaac aagggctttg cttttattca
     2161 ctacctttat tttcctattg aattagcttt ttatctaatc agatttttag tactataaat
     2221 atatatatca gatttttcga ttt
//
LOCUS       DARBER010000206         2234 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARBER010000206 DARBER010000000
VERSION     DARBER010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.213"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..544
                     /locus_tag="SCK06_002738"
     CDS             <1..544
                     /locus_tag="SCK06_002738"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1152668.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="SCK06_002739"
     CDS             complement(900..1472)
                     /locus_tag="SCK06_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1152669.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="SCK06_002740"
     CDS             complement(1488..2093)
                     /locus_tag="SCK06_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG1152670.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          339 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ctag
//
LOCUS       DARBER010000207         2233 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARBER010000207 DARBER010000000
VERSION     DARBER010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.214"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..236)
                     /locus_tag="SCK06_002741"
     CDS             complement(<1..236)
                     /locus_tag="SCK06_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1152671.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKF"
     gene            complement(254..574)
                     /locus_tag="SCK06_002742"
     CDS             complement(254..574)
                     /locus_tag="SCK06_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1152672.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            873..1649
                     /locus_tag="SCK06_002743"
     CDS             873..1649
                     /locus_tag="SCK06_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG1152673.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1664..1867
                     /locus_tag="SCK06_002744"
     CDS             1664..1867
                     /locus_tag="SCK06_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152674.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1883..2221
                     /locus_tag="SCK06_002745"
     CDS             1883..2221
                     /locus_tag="SCK06_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEG1152675.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADNTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT          846 a          352 c          381 g          654 t
ORIGIN      
        1 tcaaattttg agtgaaaagt tgtagaagta tcgtacaaaa ctgaaaattc aaaatgagag
       61 gcgctgaaat gtgcaagttc atgagataaa tgaaaggctt ctgcagtttc accgtataga
      121 ttttcattca aaaaaatgat tctgggtttt ggatagtaga aaccaggctc tttcatctcc
      181 atataaacta ctttcaaatt gtattcgctc aacatttctt tcaatttcaa atacatacaa
      241 accatcactc caactattca ttttcctcta aagctttagc aattgcaatc gctttacgca
      301 ttgtctcctt agatatttct tttccatcaa aagaaaaaac agtatcgtct tctgataaat
      361 ccacatgttt aggggtttct ctttcttctc tacctagaag gtagtctaca gagacatcga
      421 aatagtcagc aatccgactt aattcatctg aattaggagt attatttttc cactttgcta
      481 gataaccgtt agaatatcca aaattaattt ctaactgccg tatagatatc tttttcctct
      541 tcgccaagtc ttttattatt tcataggtat tcatcgataa aacaaccttt ctaaatgttt
      601 acaaaaaaag tttagaaaaa tacgcagaaa taatttgact aattctgagt aatgagctat
      661 actatatctt gtaaacagat ttaacaacta aaaagacaac aaaaaacact attgatatgt
      721 aaatgcagac cgccaagaaa gctttaaaat caatgttttt atgtcttatt taattatgct
      781 cttagtatag agtattactc agatactgtc aattgaattt agaaaaaagt tgttaaattt
      841 gtttacgaat ataaaagaaa ggaatgagaa atatgaacac accgcaaatt tttaatttcg
      901 aacaaaacga agttcggaca attttagtaa atgatgaacc atattttgta ggcaaagacg
      961 ttgcgagtgt tttgggttat tcaaacacta aagatgcttt gtcgcggcat gtagatttgg
     1021 aagataagat ggggtcgcga atcacgacct caggtcaatc aagagagatg acaatcatca
     1081 acgaatctgg tttgtacagt ttaatcttaa aatcaaaact tccctctgcc aaaaaattta
     1141 aacgttgggt aacaagtgaa gtgttgccag caattagaaa acatggaggt tatctaactc
     1201 cagaaaaagt agaagaagct ttgcttaatc cagatacaat cattcaatta gcaactcaac
     1261 taaaagaaga aagaactgga agattaatcg cagaacaaaa gattgcagag tacgaaccta
     1321 aaatctccta tttagatagc atattatctt ctacagattc agtaacaatt agtcagattg
     1381 cagcagatta tgggatgtct ccacaacaga tgaataaatt acttcataaa ctaggtattc
     1441 agaaaaaagt cggtaaccaa tggttattgt gcaaaaaaca catgaaccaa ggatacacaa
     1501 aatctcatac aactgagatc ccgaaagccg atggtggcac taaaattgta atgaatacca
     1561 aatggacaca gaaagggcgt ctatttatct acgaattact aaaaaaagaa ggatattacc
     1621 ctcaaatgga tttagaggaa attggttaga aaggagtttt agtatgactg acattgcaga
     1681 aatcactcaa cgagatagag aaaaaatcaa agaatatgtc gaaagttcaa agttcttaac
     1741 ttacaccatg cttgctgaaa gatttggaat tagcaaaagc tacttatctt taattttaaa
     1801 cggtaaaaag acttctgcag aagcaaacag aattatagat tcgattatca ctatgtacga
     1861 attgtaaagg gaggaacagc taatgcaata tctagaagca aaaatcccaa ttccagaagg
     1921 ctatgtaatt atctcccaag tggattatga ggagttaaaa aaagctgata atactggtag
     1981 atggatgacg ttgccagaag tgctagaacg gattaacaga aaatatgatt ggtttacttc
     2041 tagagtttta aagaacccaa gatatagaaa tattatcgat atcgagaaaa acaaaaatgg
     2101 atttgtctat tatccagttg aaggaagaga cacatatcta tttttaagaa gtaaaacact
     2161 tgaattttta gaaacaaatt tttcggaaat cttaaggagg caagcggatg ggaaaattta
     2221 atagagcgct agt
//
LOCUS       DARBER010000208         2231 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DARBER010000208 DARBER010000000
VERSION     DARBER010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.215"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            100..666
                     /locus_tag="SCK06_002746"
     CDS             100..666
                     /locus_tag="SCK06_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1152676.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            811..898
                     /locus_tag="SCK06_002747"
     tRNA            811..898
                     /locus_tag="SCK06_002747"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Ser,seq:cga)
     gene            1232..1798
                     /locus_tag="SCK06_002748"
                     /pseudo
     CDS             1232..1798
                     /locus_tag="SCK06_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            1911..>2231
                     /locus_tag="SCK06_002749"
     CDS             1911..>2231
                     /locus_tag="SCK06_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1152677.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT          791 a          350 c          419 g          671 t
ORIGIN      
        1 tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata gggtacaata
       61 aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa agtctatacc
      121 aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt taaaggattt
      181 actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta tctggaattc
      241 aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca actaaaagaa
      301 gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt gaactatatc
      361 tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga acatggggac
      421 ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa agaagatgaa
      481 agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac atgggtagca
      541 gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga agaacaaatc
      601 attgcactga tcaaacggac aattgactcg attttagcat tagataatcc tgaaataatc
      661 agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa actgtcaatg
      721 gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag gcttgacatt
      781 tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta atgcaccgga
      841 ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt accttcctta
      901 tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga acggccaaac
      961 aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat gcaagctttt
     1021 gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc cccttattgt
     1081 tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt ataaatgcta
     1141 gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta agataaaagt
     1201 gtaaaaacga tttgttttag gaggggaaac gatgtgtacc gcaatcactt atgcaacgaa
     1261 agaccattat tttggaagga attttgatta tgagatgtct tataatgaag tggtcactat
     1321 tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata aacattatgc
     1381 aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg ctacaaatga
     1441 aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata aagaattaca
     1501 agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag ggcaatgctc
     1561 aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata tcgattttag
     1621 tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa aatcgatagt
     1681 aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg tgttgacaaa
     1741 taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc tatcaaatgg
     1801 aacgagagta taaaatattt tgtgtaaatg aaaaaatcca tacaaaaaag gaagtccctt
     1861 ctgtagaata aagttaacga caaccaattc acagaaaaga ggacttccct atgaatgatt
     1921 ttactacaga aattgtgcaa actctagtca ctaaaggcga tttaaatgaa ttattccgtt
     1981 cgcacttaga aaaagcgata aacacactcc tacggactga attaacggct tttttagatt
     2041 acgaaaaata tgatcgcact ggttttaatt caggtaattc gagaaacggt tcttactttc
     2101 gatcaatcaa aaccgaatat ggtgaattaa cattggaaat acctagagat cgtaatggtg
     2161 agtttaaaca acaaacttta ccagcctaca aaagaacaaa cgatacattg gaaaccacta
     2221 ttatccattt a
//
LOCUS       DARBER010000209         2161 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DARBER010000209 DARBER010000000
VERSION     DARBER010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.216"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(63..488)
                     /locus_tag="SCK06_002750"
                     /pseudo
     CDS             complement(63..488)
                     /locus_tag="SCK06_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(597..1628)
                     /locus_tag="SCK06_002751"
     CDS             complement(597..1628)
                     /locus_tag="SCK06_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1152678.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          673 a          349 c          371 g          768 t
ORIGIN      
        1 gttgatttac caacatgttt agacattttc ctctaggaaa aaattaacta gcaccacggg
       61 ttaggaatct gttttgattt tgttccattt gaaatttctc tcactcgctt ttttgtctca
      121 tcatcaaata aaatgaaagg gtcttctaaa cgatcgataa atacttccat tgttgttgtt
      181 ggtagatgat aagaagtcat gacaggcact gcaccaaggt aagacacaga aacagctaga
      241 aggtatgtgt caaaagcgga actcttataa ataataactt tgtctccgga tttcactcca
      301 agatgagcca attgataagc ccgtttcaac acactttcgc agcatttttt gtaagttgtt
      361 tcaagtccta attctgggaa agctgaaagt ggctcatcaa agataatcgg ggtctcggaa
      421 aagttagctg cagcttcttg aaaattcgta aataagttca aaggggaata cgttatggtc
      481 tccatcattc acactccatt atcttttgtt aagtaatata caaattcaag ataatcgtaa
      541 cataatcctt tatataataa catagtttat tgtgaaaaaa agatcataca tttgttttat
      601 atggaagaaa cccgaacgaa tgccgctaat tcgtgataag aaaaatcaac catcttttcc
      661 cagatatctt gaaaagaatg ggatgagagg tatcgattac cacataaata atttaccgcg
      721 tcagcatcat ctaaatatag ttctgcataa taacgatatg tttttctgca tagttctggc
      781 gatacgtttt tatatactgt aattttgctc aaaatttggc gaatagtccg aggagaaatt
      841 tgctgatcat gtgaggaacg aaaaacaaaa gaattttcgt ccgtattttg tgctgttaac
      901 ttaaaatagt tctccagaaa tggcatcgct tcttttggta ttggtacttt attcagtctg
      961 ttttcttttc tattcagatt aagaatctgc ttggtcaaag atacatcttt acgtttaagt
     1021 tctactactt catggatctt taatccgcaa tacagaagaa tggcagtaat tgcaaggttc
     1081 ctcggtctat tcttttcttc aaagctagct tctctgcttg tttgaaagcc ttcaatcggg
     1141 ataaacaaat gctcaaaatc tttgaaaagt tctgcatcga tatatcgcgg agctgctttg
     1201 gttgtttccg gtaaacttgt acccaaaaga gaaatctctt cttcaatcag tcctttttga
     1261 tacaaaaatc gtaaaaaatg atttaatgaa tattgcttcc ttgaaatccc gtaatagcta
     1321 ttttcaaaca attgctccgt ttcttggttc gctgtagttt ggaacgaacg agtatgtcga
     1381 tagattttag ctaagaaatt ctgataatct tgtttttcta aatggataat cagctgatca
     1441 tccacttctt cagaaaatac ttttctcaaa taatcagtaa acagatgaaa atcttttgcg
     1501 tattcatagc gactgcctaa tgaaagggaa gaacgatttt cttctaaaaa ctgttgaaag
     1561 acggaaggga gcaagtgtgt acgttgcttt aacttttctt gcagttgttt tctagttttg
     1621 ctgtccatta caaattccct ccaatagtgt tcattttcta tattttaccg catttcatca
     1681 aatgaagata gacagtttgt cttgatctta ggaagcaaat ttttaactaa gtactagcta
     1741 aaagatatga agtaagaaga tacattttta gttattttat tagtgcagtc attcttttga
     1801 tcttttaatt gatttatcct ctttttagct atgtggttac ttcatgagca atgaaatgga
     1861 tttggttcgt tctaatggca ggaggctctt tcttttattt ccgaaaaatt agaaaacatt
     1921 ttttcagaat cgattggctc tccatttatt caataatggt ttgtctaacg atattgttgg
     1981 gataattctt aaagaagtat tagaaatagt tggtacttca ttactttaca tgaagattta
     2041 ttctatggta ggagcaatta tattgagaat taatcctttt ttatataagt tgtttgctta
     2101 ttgatggatt ggataaatta aaccatcaca acgagtggaa attatatgaa atttttcata
     2161 a
//
LOCUS       DARBER010000210         2102 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DARBER010000210 DARBER010000000
VERSION     DARBER010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.217"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..1539
                     /locus_tag="SCK06_002752"
     CDS             169..1539
                     /locus_tag="SCK06_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG1152679.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="SCK06_002753"
     CDS             1560..1844
                     /locus_tag="SCK06_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152680.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          620 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 ca
//
LOCUS       DARBER010000211         2058 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DARBER010000211 DARBER010000000
VERSION     DARBER010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.218"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..337)
                     /locus_tag="SCK06_002754"
     CDS             complement(<1..337)
                     /locus_tag="SCK06_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1152681.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="SCK06_002755"
     CDS             complement(404..559)
                     /locus_tag="SCK06_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1152682.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="SCK06_002756"
     CDS             1176..1397
                     /locus_tag="SCK06_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152683.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="SCK06_002757"
     CDS             1398..1655
                     /locus_tag="SCK06_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152684.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="SCK06_002758"
     CDS             complement(1791..>2058)
                     /locus_tag="SCK06_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1152685.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DARBER010000212         2040 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DARBER010000212 DARBER010000000
VERSION     DARBER010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.219"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1872
                     /locus_tag="SCK06_002759"
     CDS             <1..1872
                     /locus_tag="SCK06_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1152686.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DARBER010000213         2004 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DARBER010000213 DARBER010000000
VERSION     DARBER010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.220"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SCK06_002760"
     CDS             <1..378
                     /locus_tag="SCK06_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1152687.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SCK06_002761"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SCK06_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1152688.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2004
                     /locus_tag="SCK06_002762"
     CDS             1859..>2004
                     /locus_tag="SCK06_002762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152689.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFT"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DARBER010000214         2000 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DARBER010000214 DARBER010000000
VERSION     DARBER010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.221"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..219)
                     /locus_tag="SCK06_002763"
     CDS             complement(<1..219)
                     /locus_tag="SCK06_002763"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152690.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="SCK06_002764"
     CDS             complement(330..584)
                     /locus_tag="SCK06_002764"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152691.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="SCK06_002765"
     CDS             complement(777..1052)
                     /locus_tag="SCK06_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG1152692.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="SCK06_002766"
     CDS             complement(1024..1977)
                     /locus_tag="SCK06_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1152693.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          603 a          368 c          294 g          735 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact
//
LOCUS       DARBER010000215         1992 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DARBER010000215 DARBER010000000
VERSION     DARBER010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.222"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="SCK06_002767"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="SCK06_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1152694.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="SCK06_002768"
     CDS             852..1040
                     /locus_tag="SCK06_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1152695.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="SCK06_002769"
     CDS             1053..1610
                     /locus_tag="SCK06_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1152696.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="SCK06_002770"
     CDS             1642..1884
                     /locus_tag="SCK06_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1152697.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          554 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ct
//
LOCUS       DARBER010000216         1865 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DARBER010000216 DARBER010000000
VERSION     DARBER010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.223"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..543
                     /locus_tag="SCK06_002771"
     CDS             <1..543
                     /locus_tag="SCK06_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152698.1"
                     /translation="IPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGID
                     ETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLI
                     SPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEE
                     KILLPENWAELRKLVEYGII"
     gene            530..>1865
                     /locus_tag="SCK06_002772"
     CDS             530..>1865
                     /locus_tag="SCK06_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1152699.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIR"
BASE COUNT          617 a          241 c          286 g          721 t
ORIGIN      
        1 attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat ctttgggata
       61 gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt atctgatggt
      121 tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc tggaaaaaaa
      181 gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga aaaaaacata
      241 ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc ttcaacactt
      301 ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga aaagacgatc
      361 aactctatta tgttggctaa attaatttta aatgaatata cagcattatc tgaagataaa
      421 aaagaaacat tgacaaatat tgttcttttc tgtgaacatt ataaccgtct atcagaagaa
      481 aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt tggtagagta tgggattatt
      541 taacacactt tccaagtcaa aattagttaa gtccagtttc tggtatacta tagggaattt
      601 ttttattaag ggaatttcat tcataactat tcctattttt gtaaatatta ctactcaagc
      661 agaatatggt ttgattaata atttcacttc atttgtatca attttttcga ttcttgttgg
      721 gctaagccta aattcggcaa ttaataatgc aagttttgac tataaagatg acataaatga
      781 atttatgtca tcggtacttt ctctagctac actttcgtta attgtgttct tattaatagg
      841 gaacatatat tttttgtttt caaatgcttt ttttgatatg aaccaacttg tatttaattt
      901 gatgttatta caaagttttg ctagttttgt aattagttat atccagtctt attttacgat
      961 taactttaac cattttaaat atttgatttt atcttttata tctacttttt tgaatattgc
     1021 aatctctgta gctttgatgc agacattata ttttgatgat aaatatgttg gcagagtagt
     1081 cggcggaaca ctgtcatttg tgttgatagg tttagtaatt tatttaagaa taatgataaa
     1141 gggtgctcat ttttttgata aaaaacagtg gagatacgct ttaaaaatct ctttgccgct
     1201 aatcccacat tccttagcaa atatagtttt agcacaattc gatagaatta tgattaattc
     1261 ttatttaggt cctgctgcgg ggggaatata ttcctacatt agtaacatag gtattattct
     1321 aagcgtaata tggatgtcta ctaataacgc ctgggttcca tggttctatg gtgagatgag
     1381 taaaaataat gaaaaaaaag tgaagaaagt tagtaactat tatttagtag tctttacttt
     1441 gattacgatg attgtaatgg tagtttcgat tgatttagca aaatttatgg ctccgaaaga
     1501 ctattattct gggttatctt tagtggttcc gataacctta ggctatttct ttcagttttt
     1561 atatagttta ccagttaatg ctgaatttta tgagaaaaaa acatcgttta ttgcgattgg
     1621 tacagtagca agtgctatag ttaatatact attaaacatg attttcatac ctaggttagg
     1681 aataattgct gctggataca caaccgtatt tacgtatttt ttacttttta tttttcatta
     1741 taatatcgct aaaaaaatta cgaataaaca attatttgac acaaaaaaaa tatgtatcat
     1801 aataattttt gtatcaattt tttctttgat acttcatatt ttaatcaatc aaatgttgat
     1861 tcgtt
//
LOCUS       DARBER010000217         1819 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARBER010000217 DARBER010000000
VERSION     DARBER010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.224"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..258
                     /locus_tag="SCK06_002773"
     CDS             <1..258
                     /locus_tag="SCK06_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEG1152700.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
     gene            356..565
                     /locus_tag="SCK06_002774"
     CDS             356..565
                     /locus_tag="SCK06_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEG1152701.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            582..854
                     /locus_tag="SCK06_002775"
     CDS             582..854
                     /locus_tag="SCK06_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1152702.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            856..1719
                     /locus_tag="SCK06_002776"
     CDS             856..1719
                     /locus_tag="SCK06_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEG1152703.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT          731 a          289 c          332 g          467 t
ORIGIN      
        1 atgatcggtt ttgttcctta tttagttgat acggacagcg caacgataaa atcaaacctg
       61 gaagaactgt ataagcaaca taaagaagat aacttggttt tccaaaatat tatcaagcga
      121 agtaataaag taagtacctg gtctaaaaac ggcattacag agcataaagg ctatgacaaa
      181 aaagttttat ccatgtataa gaacgtattt tttgaaatgc ttgagcgaat tattcaatta
      241 gaaaacgaaa aagaatagaa tcacaaatca caagtgatta atcacaaatc acttgtgatt
      301 tgtgattgtt gatgataaaa taagagtaag aagaaataga aagaagtgag tgattgtggg
      361 aaatttaggc gcacaaaaag caaaacgaaa tgatacacca atcagtgcaa aaaaagatat
      421 aatgggagat aagacggttc gtgttcgtgc tgacttgcac catatcataa aaatcgaaac
      481 agcaaagaat ggcggaaacg taaaagaagt tatggatcaa gccttagaag aatatatacg
      541 gaaatattta cctgacaaac tttaaaagga gtgaaaatga aatggcagtt acgtatgaaa
      601 aaacatttga aatagagatc attaacgaat tatcggcaag cgtttataat cgagtattaa
      661 actatgtttt gaaccatgaa ttaaataaaa atgactctca attattggaa gtcaatttat
      721 taaaccaatt aaagcttgca aaacgtgtaa atctttttga ttattcttta gaagaattac
      781 aagccgttca tgagtattgg cggtcaatga atcgttactc aaaacaagtt ttgaataaag
      841 agaaagtggc ttaatatggc aaatatagtc aattttactg acaaacaatt tgagaatcgc
      901 ttaaatgata atttagaaga attgattcaa ggaaaaaaag cggttgaatc gccaaccgct
      961 tttttacttg gtgggcaacc agggtcaggg aaaaccagtt tgcgatcagc aatttttgaa
     1021 gaaacacaag ggaatgttat tgtcattgat aatgatacct ttaaacaaca gcaccctaat
     1081 tttgatgaac tagtgaaact ttatgaaaaa gacgtagtaa aacacgttac cccttattct
     1141 aatcgcatga cagaagcgat cataagccgt ttgagcgatc aagggtataa tttggtgatc
     1201 gaaggtacag gacgaacaac agacgttcct attcaaaccg caacaatgct tcaagccaaa
     1261 ggttatgaaa caaaaatgta tgtcatggca gtacctaaaa tcaactcata tttaggaaca
     1321 attgaacgat atgaaaccat gtatgcagat gatccaatga cagccagggc aacaccaaaa
     1381 caagcgcatg atattgttgt caaaaactta ccgaccaatt tagaaaccct tcataaaacg
     1441 ggcttattta gcgatataag gctatataac agagaaggag taaaactcta ttcaagtttg
     1501 gaaacacctt ctattagtcc aaaagaaacc ttagaaaaag aattgaatcg taaagtatca
     1561 gggaaagaaa ttcaaccgac tttagaacga atagagcaaa aaatggttct aaataaacac
     1621 caagagacac ctgaatttaa agcaattcaa caaaaattgg aaagcttgca gccacctaca
     1681 ccaccaatac ccaaaacacc taaacttcca ggtatttaaa ccttaaaaaa ggaaaagagt
     1741 agttaccaaa aaacggtaac tactcttttt ttataaaaac atttcctcat gttggggtcc
     1801 cgagcgccta cgaggaatt
//
LOCUS       DARBER010000218         1775 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DARBER010000218 DARBER010000000
VERSION     DARBER010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.225"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            134..532
                     /locus_tag="SCK06_002777"
     CDS             134..532
                     /locus_tag="SCK06_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1152704.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            516..1211
                     /locus_tag="SCK06_002778"
     CDS             516..1211
                     /locus_tag="SCK06_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG1152705.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          674 a          258 c          344 g          499 t
ORIGIN      
        1 cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat gtgttattca
       61 ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg atattttacg
      121 aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta tagaagcaga
      181 agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg aaacattaac
      241 agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt gtgatatgaa
      301 gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg tccaaactaa
      361 tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc cggaacttcc
      421 aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga caatcattaa
      481 aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt gatacagcaa
      541 atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta acaacaaatc
      601 ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa gaaatcaaaa
      661 aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa gaagaaatga
      721 tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc aaggtgccgg
      781 tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat ttgattaccg
      841 gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga gcagattata
      901 ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa gtgattgatt
      961 cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca gctagtttca
     1021 aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata acgttaggta
     1081 aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc
     1141 aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg
     1201 attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag
     1261 gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac ttatttctca
     1321 tttaaacgat gtattcccta acctctaata catccttgta actctgaagt tacgccagtt
     1381 ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt tcgtgtttct
     1441 ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct tttttaaaat
     1501 aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg gcaagtgaaa
     1561 aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga tagaaataaa
     1621 aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt tttggtacaa
     1681 ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa taagaaggaa
     1741 tttccaaaaa tcacttctca aattttgcca catcc
//
LOCUS       DARBER010000219         1729 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DARBER010000219 DARBER010000000
VERSION     DARBER010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.226"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            280..1320
                     /locus_tag="SCK06_002779"
     CDS             280..1320
                     /locus_tag="SCK06_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1152706.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1569..>1729
                     /locus_tag="SCK06_002780"
     CDS             1569..>1729
                     /locus_tag="SCK06_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1152707.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          611 a          209 c          371 g          538 t
ORIGIN      
        1 ccagtttagg ggtatatatt ttacatgaaa atcattattt cattgatggt atgaagtcta
       61 gaggcttaat atgggatggg atatttggat taggcaaaag gtttttagat agggattttt
      121 ggatttggtt tataattgca gttgtagttg tgtgcataat ttgcatggct atcgatgttg
      181 taagagaaaa atttgaaaga cagattttta aaattaagag tgtaaagaac ctgtgtaaac
      241 ttatgaatca tgttataaat atatacccat aattaataaa tgaggagaaa agaagtgaaa
      301 gttatatcat tcggagaatt attattgaga cttgcagcac ccggttattc aaagttattt
      361 caaagagata gtttggagac tacattctgc ggaggggaag ctaatgtagc ggtatcctta
      421 gcaatatttg gatatcaatc agaatttata acaaagcttc cggattcaga tgttggatat
      481 gcggcagcta atgctttgcg ttattttgga gtaaatgttg ataatattat atttggagag
      541 ggacgcatgg gactgtatta tttagaaaaa ggagcagctc agagaccatc taaagtaatt
      601 tatgacagaa aattttctgc aatttcgtta gctgatagtg atgaatttaa gtggaaggag
      661 atcctaaacg atgcaaattg gtttcattgg acagggataa atccagcatt gtctgagaat
      721 ctctttgaga cgtccttaga tgcatgtcag gctgccaaaa aaagaggtat aacaatttct
      781 tgtgatttaa attatcgatc caagctttgg acatcagagg aagcaaaaga aaagatggaa
      841 cagatactac cattggttga tatttgtatt tgtaatgaag aagatgcaga aaaagtgcta
      901 ggaattaaag ctgagcatac aaatattaat agtggagaat taaataaaga tggctatgtg
      961 caggtggcta gggagattca caagaagttt ggatgtaggt atgtagctac aactctgcgt
     1021 agaagttatt cggcaagtag aaatggttgg agtgcaatgt tatacgaagc agaaaaagat
     1081 aaagcgtatt tttccacaga atacaacata caaattgttg atcgcgttgg tggtggagac
     1141 agcttttctg ctggtataat atatgcgtta tgtgaaaata tgtccggaca aaaggctatt
     1201 gaatttgcga ctgcatctag ttgtttaaaa catactatag aaggtgattt taatcgaact
     1261 acaattgagg atgtatataa tttaatgaat agcggtggaa atggtcgcgt gttgcgttag
     1321 tcaacatata aagataaatc acagtaattt ttatggtgat tatctgtctt agtagtgatt
     1381 agaataacgt gaaaataaat tgttgagcgt ttatactgtt ttgaaaaata aatttaacat
     1441 attgaaaaat acaaatatat acgtatatta taaagcgccg attgtaaaat taagctagac
     1501 aactaaaaca gtcatttgtg ctacactcaa aatagttccg accaaagaat tataaggagt
     1561 gagtgcaaat aacctatacc tatcttacaa cggatgaact tgtaataata gagtcttatt
     1621 tgaaaattaa ccaatctgtt gctaaaactg cgcattgctt gaatcgttca agtcaaacga
     1681 tccataaagt atatctgttc ttcaagcaaa gaaaatcagc cttagaata
//
LOCUS       DARBER010000220         1728 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DARBER010000220 DARBER010000000
VERSION     DARBER010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.227"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..254
                     /locus_tag="SCK06_002781"
     CDS             <1..254
                     /locus_tag="SCK06_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1152708.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
     gene            387..1726
                     /locus_tag="SCK06_002782"
     CDS             join(387..828,828..1726)
                     /locus_tag="SCK06_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG1152709.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          711 a          234 c          302 g          481 t
ORIGIN      
        1 gaaacaaatt gaaggagtac aaaatctttc aaagcatgtc tcggaaaggg aactgcttag
       61 ataattcact tatggagaat ttttttggct tattaaaaca agagattttt cacgggaaag
      121 tttataactg tttcgaggaa ttaaaatcag ccatcgatag ctatatctat tactacaata
      181 atgaacggat aaaacagaaa ttaaactggc aaagtcccgt ccaatttcgt aaaacaaccg
      241 ttacggttgt gtaaaaatat cattttagag catgaaaaaa agagtggatt tctccactcc
      301 aaaaaagtct aacttttggg ggtcactaca aaatgatgtg caccccaaaa gttagactag
      361 aaatctaatt aaaaggggtg ctttttttgc ggaaatatac atttgttttt aagaaaaaag
      421 tagtttcaga ctatttaaat aacgaaggcg gctacaaata tcttgcacat aaatatcaaa
      481 taaatcgtac actggttaga cattgggtaa ggatttataa ctatcatggt tgggaaggct
      541 tggttggagg tggcaaaagc tacactacaa aatttaaact tgatgttata gaatatatgg
      601 aaacaaatgg tctttctatc caagaaactg ctaaaaaatt taatatcggt tcaaatagaa
      661 ctctaagtaa atggatagag caatatgaag aaggcggtgc ttcttcactt gagagccaaa
      721 aaaggggcag aaaaattagt atgaattcca agctaaacat tcctaaaaaa cttaaagatg
      781 agtctcttga agaagaagtt attcgtttaa gagcagagaa cgcatattta aaaaagttag
      841 aaaccttgat tcaagaacaa gatttgagca agaagaaatc aaggttaaaa caatccttca
      901 gctcaaaaaa gaatttaaat tgaatctatt actttcaatt gcacaattag cgaagtcaac
      961 ttattattac tgggtaaaaa aattagataa gccagataaa tatagcaaga taaagcaaga
     1021 aattacagcg attgtaaaag aatcaagaaa ctcttatggt tatcgtagag tcactttagc
     1081 gttaaagatg aaaggatata caatcaatca taaaacagtt agaaaattaa tgtcccaaat
     1141 gggacttacc tgtcaaatcc gaataaagcg atataaatct tataaaggaa cagtaggaaa
     1201 aattgccaag aatgtgttaa aacgaaattt ttcagtagat acacccaata aaaaatgggt
     1261 gacagacgtc actgaattca agataaaagg aagaaaaatc tatctatctc ctatacttga
     1321 tttatttaac ggagaaataa ttagttatag tatatcaact agtccaacat ataaactgat
     1381 tgaagaaatg cttcaacaag caattaaaaa aaagggaact gagggttcac tgattctaca
     1441 ttcagatcaa ggatggcagt atcagatgcc acaatatcaa aaaaaattaa aagagaataa
     1501 cattattcaa agcatgtcta gaaaaggaaa ttgcttagat aattctgtaa tagaaaattt
     1561 ttttggtgtg cttaaatcag agttttttta tcgagaaaaa ttccgatcaa ttgagatatt
     1621 tcaaagtaaa ttaaatgagt atattaggtg gtataataac aaaaggataa aactaaagtt
     1681 aaatggacta tctccagtag aatatcggaa acagtccatt aaatagtt
//
LOCUS       DARBER010000221         1664 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DARBER010000221 DARBER010000000
VERSION     DARBER010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.228"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..505
                     /locus_tag="SCK06_002783"
     CDS             131..505
                     /locus_tag="SCK06_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1152710.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="SCK06_002784"
     CDS             complement(619..1248)
                     /locus_tag="SCK06_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1152711.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="SCK06_002785"
     tRNA            complement(1382..1454)
                     /locus_tag="SCK06_002785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DARBER010000222         1661 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DARBER010000222 DARBER010000000
VERSION     DARBER010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.229"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..332)
                     /locus_tag="SCK06_002786"
     CDS             complement(<1..332)
                     /locus_tag="SCK06_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1152712.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEE"
     gene            complement(466..1656)
                     /locus_tag="SCK06_002787"
     CDS             complement(466..1656)
                     /locus_tag="SCK06_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEG1152713.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT          475 a          342 c          311 g          533 t
ORIGIN      
        1 aattcttcta agattgtttg actttcagaa tttaaagaaa tggaagcaca aatctcattg
       61 tcaaattctt cttcgctgat tccttcaaat gtcgcagaga taataatcca atgcccttgc
      121 cacccatttt gaattccatg aaaaaaatct gattctttta gtgttttact actatagtaa
      181 taactatcat ctaataagat tctcaatgca gtaagagcat tggttttccc tgaatcattc
      241 tcacctataa tagtatttgt ctcattatcg aatcgaaaga tttcggattc ataatttcta
      301 aaatttcgaa tttgaagagt agatataaac ataagcagat ctcctaacta tttaattaat
      361 atgattatat agtaagttta tcataaatta tgttagtaaa agttctcatt ataagggagc
      421 gtcaataatt ttgtgtaaat gattcttccc tactgcagat tgtttctaat tctcaatcag
      481 ttggcttagc atttgttcga gctcgccttc ggcctgttgg aaacctttat gactgcgacc
      541 taggaatttt tggttgtaag tatcaaaaga cgagactaag aaacgttcca tcgactcttc
      601 attttggaac tgttccttgc ggtggctgta tttcttgatt tgtttattaa aggattcaat
      661 caagttcgta gaataaatac tcttgcgtat ggccaagggg aaatcataga aagtgagcaa
      721 gtgatcgttc gaaagaatcg attcaaccac ttttggatag ctggttttcc atttttccgc
      781 aaaagtacca cgtgcttcca acgccacctc tttagatgag gcttgataca ccattttaaa
      841 atcatcacag acttccttac gatcatcgac acgcacttta tgactgatat tacgggaaac
      901 gtgtacacaa caatgttgaa aacgagcttt gggatagaac cgactaatcg ctcctaccat
      961 cccctttaag ccatccgtga tgaacaggag gacatttttc aaaccgcgct cttgaaggtc
     1021 caataaaatt tcctcccaaa tcgtgatgga ttcagtcggt gcaatcgcat agctcaatac
     1081 ttcctttgat ccgtccgggc gaatgccaac tgcgatatga atagcttcct tggcgacggt
     1141 tttccgcttt aacggaatat acgttgcgtc catataaata gcagcataac ggtcatgaag
     1201 ctcccgccca ttaaacgccg ttacctcttc tgtaaatgat tttgttatat tggacatggt
     1261 ttggggcgtg tagtaatgcc catacatttt ctcaatcaag tctgcgattt ccgacatggt
     1321 aatacctttt cggaagaggt gaatgacggt ctcctctaac gtgtcattcg tccgtctata
     1381 agcaggaaca gtctgttgct tgaactcacc attgcggtcg cgcggaatct ggagatgaag
     1441 ttccccatac tcggtcttga ccgtacggtc ataggagccg ttacgagaat tacccgtgtt
     1501 aaaaccaatg cgatcatact tttcgtaatc taggaaagcc gttaactccg ttttgagaag
     1561 cgtattgact gctttctcca aatggacacg gaataattca ttcaaatcgc ctttgttcgc
     1621 tagagtcttt agaatttctg tagtaaaatc gttcataggg a
//
LOCUS       DARBER010000223         1625 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARBER010000223 DARBER010000000
VERSION     DARBER010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.230"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            30..290
                     /locus_tag="SCK06_002788"
     CDS             30..290
                     /locus_tag="SCK06_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152714.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(652..891)
                     /locus_tag="SCK06_002789"
     CDS             complement(652..891)
                     /locus_tag="SCK06_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152715.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1177..1491
                     /locus_tag="SCK06_002790"
     CDS             1177..1491
                     /locus_tag="SCK06_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152716.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          562 a          220 c          286 g          557 t
ORIGIN      
        1 aatgaaaaaa cattaaggat ggatgaaata tgaataacga aactatcatt ttaggaaaaa
       61 aatatatttg ccaacctatt ggcttgaaac aaaaagtggt tggagaagta atcagtaaat
      121 tagaaaattg tgttgtgatc tgcgtagacg aatatcatga cgtcgatcgc aatgaaatat
      181 tagaaaaatg cggaaaagta gtggcaaaat atactcacgt ttatgagttg gaaacaacag
      241 aatatctata taactatagc aacaaatatg aacatacttt agtttcataa agatttaaat
      301 aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc actttattta gttggcagtg
      361 attgttcatg atttttgatt tattcaaaag gttcgcctag agaatctgtc tctgtaacga
      421 ggcctggtgg agaaactcga taaaaaccta tactgccaga actgtcctat tttcggatag
      481 acaaactagt agttttaaaa agatcatccg atccaatcat ttcaaaaaat gcaaaactga
      541 tatcaggatc atttagctca ggcttgttat cgtaaagcat ttgtaaaggt tgcatttagt
      601 taggagtaaa cgcgaaacta tcatcattat ttgctgaaga agatgaagaa gctaattcag
      661 agtttgtttg aaataaagga gacgtgctga cagtttcttt accctgtttt ttgcagaggt
      721 agaaactttt atatcggaag tttctaccgg agaatttttc tcatttgtag aaattgttga
      781 atcgatacta ttcgaaattt gcgtatctga aggtatgttc tttgatgcag aagtcccaca
      841 agcagtaagt aacaataaat ttgataaact gataaacaga attcttttca ttatgtacac
      901 actccttata gtatcgttta gcatacacct atgctcagta ttaggcaaaa tcaatgcaaa
      961 actatcaagt cttttatagt atctattagt cttagtaata catatatgga gagggtgata
     1021 tcccagttca aaccagatac attggttacc atctgtatta acgattatgc ctctaaacgc
     1081 tttttttatg aaaaaattta taagtgaaaa cgtaggtatg atattatcta gaggtaattt
     1141 ttttgggtat ttaaatagtt gctaggagga tagcttttgg atttttacag taattttatc
     1201 ttaataatag caatattatt acttctaaat atttggtttt ttgataagag tagaaatgca
     1261 gggataggtt ttagaaccaa acgaagtacg tcttctgaaa aaaagtgggt atattcacag
     1321 actatttttt acggaggggt tatttcaatc agtttacttt cttcaacact ttattctttt
     1381 aatgttatag acgtttccat gtcaaatttt atatctatta ttggaatcct tatttctgca
     1441 attattacac aactacttct tgtgtttgaa gagaagtcaa aaaataatta aagcaattat
     1501 gccttttagt acaggaatta ttacttctta caaatgtata gcgaatgtga agcctgctgt
     1561 tttaattgtt ctagaaaaac ttttgagtga acaattattt tatgttagga tgaattaatt
     1621 tctct
//
LOCUS       DARBER010000224         1561 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DARBER010000224 DARBER010000000
VERSION     DARBER010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.231"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            51..1517
                     /locus_tag="SCK06_002791"
     CDS             51..1517
                     /locus_tag="SCK06_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG1152717.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          434 a          290 c          329 g          508 t
ORIGIN      
        1 gtaaagatgt ataatatttt ctgaaaattt aaagaattgg ggtatagatt atggaaaaac
       61 agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc ttcactgaga
      121 tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat atgtacgata
      181 cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc atgtcgatct
      241 acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac cgagtgttag
      301 gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac atcgtcttgg
      361 ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt atcggaaccg
      421 cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag actgacttgc
      481 gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca ttattagcac
      541 cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc tctttagctg
      601 ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag acattagacg
      661 gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga ttcatccgta
      721 acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa ctaactggtc
      781 atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt
      841 attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat
      901 gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa gaacaaggtt
      961 catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt ggtgtaacga
     1021 tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg acaccagtat
     1081 tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg aaattctctc
     1141 ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt atgctttatg
     1201 gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc gtggttattg
     1261 ctgaattgtg tctttcacca gttggtttgt ctgtgacaac aaaattagca ccacgagcat
     1321 ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa gcaatcaatg
     1381 cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt attatcggtg
     1441 cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc aaaaaactaa
     1501 tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg cagcagttcc
     1561 t
//
LOCUS       DARBER010000225         1493 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARBER010000225 DARBER010000000
VERSION     DARBER010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.233"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1269
                     /locus_tag="SCK06_002792"
     CDS             <1..1269
                     /locus_tag="SCK06_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1152718.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVE
                     IGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKV
                     PVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTA
                     GNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKN
                     KEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVD
                     PITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATD
                     ESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGES
                     DSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          591 a          219 c          320 g          363 t
ORIGIN      
        1 aattacgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctgac gccgataaat atgaaccaac agtagaaggc
      121 gaaaaagtag aaattggagg cacagtagat ttaacagata atgttactaa cttgccagcg
      181 ttaccagaag gaacaacagt aacagatgtt actcccggcg gtacaattga taccaacaca
      241 ccaggcaatt atgaaggtgt cattgaagta acgtatccag acggtacgaa agatacggtg
      301 aaagttccag tagaagtgac agacaatcgt tctaatgcag ataaatacaa tcctgaagga
      361 caaaaagtga ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa
      421 gaagatttac caaaaggaac tacttatgct tggaaagaga aagtagatgt tagcacagct
      481 ggaaataaaa aaggtactat tgtagtgaca tatccagatg gatcatctga tgaagttgaa
      541 gtagacgtta cagtgacaga tactcgttcc gatgcagata aatataatcc tgaaggacaa
      601 aaagtgacta cagacttaaa taaagagcca gatgcatctg agggaattaa gaacaaagaa
      661 gatctaccaa aaggaactac ttatacttgg aaagaaaaag tagatgttag tacagctgga
      721 aacaaaaaag gtactgtagt agtaacatat ccagatggat ctaaagaaga agtagaagtt
      781 actatttctg tagtagataa aaaagcaccg aataaacctc aagttgatcc tattacagag
      841 ggtgaccaaa ttgttactgg taaaactgaa ccaaatgcag aggtgacagt tacattacca
      901 gacggaagtc aacaccatgg gacagctgat aaaaatggta attttacagt taaagttcct
      961 aaattagagg cagggacaaa agtgatagta actgcaactg atgaatctgg taatactagt
     1021 gaacctacta atgtagttgt ttcttccaac aaaaaagaca gtggaaaaaa cggtagtaaa
     1081 ggtagtaaga ctgacaatca aggtagcaac agcaatcaag acaaaaatag aggcaaaagc
     1141 cagtcctcaa aagtttttcc taagacggga gagagtgaca gtaatatctt tactattagc
     1201 ggtggtttaa tactgttagg aacgttagga ttattagggt ataaaaatcg aaaaaaagag
     1261 aatgaataga aaatcaattt cttaagtctt ctctgagaag aggaagaatc tttttcttcc
     1321 tctttttgct tttttatgat gggaaataag tgttagttaa agatgaatat actttttata
     1381 gaaaggaaaa gaatgtaatt aagtggaaaa aatagtaatt attggttcaa accattcagg
     1441 gctagccgca gctcgatttc taaacttaag cgattcaaat tatgaagtta ttc
//
LOCUS       DARBER010000226         1457 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARBER010000226 DARBER010000000
VERSION     DARBER010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.234"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="SCK06_002793"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="SCK06_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1152719.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          382 a          257 c          298 g          520 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttg
//
LOCUS       DARBER010000227         1451 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARBER010000227 DARBER010000000
VERSION     DARBER010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.235"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..625)
                     /locus_tag="SCK06_002794"
     CDS             complement(<1..625)
                     /locus_tag="SCK06_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1152720.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="SCK06_002795"
     CDS             complement(784..1050)
                     /locus_tag="SCK06_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1152721.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="SCK06_002796"
     CDS             complement(1040..1396)
                     /locus_tag="SCK06_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1152722.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DARBER010000228         1442 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DARBER010000228 DARBER010000000
VERSION     DARBER010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.236"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            767..1153
                     /locus_tag="SCK06_002797"
     CDS             767..1153
                     /locus_tag="SCK06_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1152723.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1251..1442
                     /locus_tag="SCK06_002798"
     CDS             1251..1442
                     /locus_tag="SCK06_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152724.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          539 a          226 c          196 g          481 t
ORIGIN      
        1 aattaaaaac cacgataggt aactttaaac aaacatgaag agactttaat aagacttctt
       61 tatctgaaaa cacttttgtt tctctctttc ctttctacat cattaaatag tattttatac
      121 gttatctaaa atactattcc tctgtcaaaa taagctaact tacttcatta taaaatatcc
      181 acttatcgct ttcatgtata ttccaccgta aaaagtacat tttatcaccg agaactcatc
      241 atttttcatc atgatgaata ctatttacat atatctagtt caaagacaat aattattatg
      301 atccgttatt atctaagcac tatacagata ataggaattt ttcgtttcta aatactccaa
      361 tttgggaatc caataaaaat tttcgttcaa atacgtgtct attcgaactc ttttctagtt
      421 gttaattatg aagggggaag aaataaaaaa tacaatctat acacgaataa gaatattatc
      481 caaaaggagg ttttagaaaa gatttattat acgtaaaaag ctcaagacat tttcttagcc
      541 ataaggacat gtttgatatt taagaagata tatgaatttt cttttctaag tataaagata
      601 actatccatt atcaactaaa aaaacatttt tgaaaataaa catcagcagt cctatcaatg
      661 catgtgagct tatagaagtt attcgcagcg cagcataatt gcattagctt cttttcgtgt
      721 tttttgcctc ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt cagataacga
      781 cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag gtcattttga acaccgctaa
      841 tacttattat aaaaaacaag aagaaattca aaaacatgaa caacttgatc aactcccacc
      901 atatttgttt actaaagata ctataaaatt taaacaaccg attaatgttt taaatgtgac
      961 tctccttgtt gaagatataa aattagcaaa aatccttcct ttgttgaaag aacctgaaca
     1021 aatttttctt atcgaaaaat ttatctttaa taaaactgat aaagaaattg gaaaggttct
     1081 ggggattaca agacagggtg cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa
     1141 aatgctggtt taatatctgc ataataacta attttgtatg cttctttgct cgtcctcctc
     1201 cttccccaaa aaataaatag attataagtt catgggaaga aggtaaattt atgaatactt
     1261 taaaagaaga tttcatttta gcaaagaatg gaaatgaaga agcagttgtg gccattctca
     1321 aaagatttag cgctttggtc cataagcaat cctggagaac tggaaaatat gatcaagatt
     1381 gttatcaaga atgtatgctt gcaatatatc tagctatctc gaaattcgaa attaaagaat
     1441 aa
//
LOCUS       DARBER010000229         1440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARBER010000229 DARBER010000000
VERSION     DARBER010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.237"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SCK06_002799"
     CDS             105..1406
                     /locus_tag="SCK06_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1152725.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARBER010000230         1424 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DARBER010000230 DARBER010000000
VERSION     DARBER010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.238"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SCK06_002800"
     CDS             complement(150..1337)
                     /locus_tag="SCK06_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG1152726.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DARBER010000231         1407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARBER010000231 DARBER010000000
VERSION     DARBER010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.240"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            132..344
                     /locus_tag="SCK06_002801"
     CDS             132..344
                     /locus_tag="SCK06_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152727.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="SCK06_002802"
     CDS             506..1024
                     /locus_tag="SCK06_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEG1152728.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..>1407
                     /locus_tag="SCK06_002803"
     CDS             1031..>1407
                     /locus_tag="SCK06_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152729.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLE"
BASE COUNT          546 a          169 c          221 g          471 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatt
//
LOCUS       DARBER010000232         1399 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARBER010000232 DARBER010000000
VERSION     DARBER010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.241"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            182..>1399
                     /locus_tag="SCK06_002804"
     CDS             182..>1399
                     /locus_tag="SCK06_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152730.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQ"
BASE COUNT          460 a          144 c          241 g          554 t
ORIGIN      
        1 aaatttactt ttatttgatg actggaaata gaaattaaat atattgtatt gttacattaa
       61 agaaaacttg tatggaaaaa tcagtacaat accattattt aatgttcttc acttaaagcg
      121 caaaaaatat cagtttatta aactcgtata tagaacaaat atatgtagac ggggcgaaac
      181 gatgagtgta aaaaaaatgc tatttatggt aagtacaata attgtattaa catcccgatt
      241 ttggggaatt aatactagtt tatctatgcg atattttatt atggcaatat gggatttata
      301 ttttatgtta agctattttt cttatcgtga gaaaacatat aaaaaatacg ttatcaagaa
      361 taacttttgg ttgactataa tgccgtttgt gatattttct atctatactc taataatatg
      421 gggaattggt tcaaatgcag aatttgaaaa ttttataaag ttatgttcca cgttgcttta
      481 tttaattctt gcatatggtt acgcatgtac agcattttac ttatttaagg cagaaggaat
      541 tgattttatt ttttggtcag gtgttatttc atatatgagt ggttcagttt tttatttaat
      601 tgtatcttat ggtttaaatg gtttaatttc ttatataaaa agtcttataa caggtgaaac
      661 aaatactgca aattttgcta tggaggtaca tgatttaact tttgcgatgg gggttttttt
      721 cctttattac gtattttttg aaaataaaaa tacaaaaaaa cataagataa aaattatcgt
      781 atctttaatt ttaattttca tgggattgaa gagaattgaa ctgttagctt taagtggtgc
      841 aattctagtg tattatgttt tattaaagtg gggaaaaaag attcaaatcc gtgcattagt
      901 gtgtactatt tgtgcaactg ttatttcgtt agtgtttatt tacattatag atacaggaat
      961 tttggcattg cttatgtcaa atttgggcat tacagatgac ggtgcgaggc tttcatttta
     1021 tctatattca tctaaatttt atgaacttgg tttaggatat gtaggaacag gatggacatg
     1081 gttttcaaaa tactttttta atctttattt atcgcacttt aggttaaatg gtggaagaat
     1141 tgcagcaagc ctacacagtg atatattaac tatttatata gaagttggat gtatagtttt
     1201 tattatatgg atgttttggt tattttttgt gaaaccgata agactaacta aaaaatttgg
     1261 attgaaaaca ggggagtgtg tattattatt aactatatat atgtttatat tatatttgac
     1321 ggataatacg ctcacttatc cagacactca aatgattttt ctcttagtac ctatgatagt
     1381 ttcgtgggtt gataatcaa
//
LOCUS       DARBER010000233         1381 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARBER010000233 DARBER010000000
VERSION     DARBER010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.242"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(266..523)
                     /locus_tag="SCK06_002805"
     CDS             complement(266..523)
                     /locus_tag="SCK06_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG1152731.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(648..980)
                     /locus_tag="SCK06_002806"
     CDS             complement(648..980)
                     /locus_tag="SCK06_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152732.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT          438 a          240 c          179 g          524 t
ORIGIN      
        1 cactaaataa cttctgattt aattctgcac ccaaaaagat acactcctat ctcttattta
       61 aagttgctat ataccctctt attttagcat actcagtttt agaaaaagca aaaaaataaa
      121 gaaacttatt cttgataaca agctacgtga cgtatatccc taacacaaaa atagccatat
      181 cctttggttt ggatacggct atttgatatt actcattcaa ttatcaaatc acagtaggtg
      241 tgaatctcat cgggatcggc aatatctact tctgattagg atcaaaggtg attttctcaa
      301 taaaggtgtg atacaaagct ttaacttgct tcttctcact ctggataaga aagcaatcca
      361 aactagtcaa tatctgttgg acatctttga cttgaatcgg ttccccttgt tgttgaaaga
      421 tccctaagat ttcattctct cgaatttgta actgtcttcg tttttcagtc aattcattta
      481 aacgatcttt taacatcgga aacaattcgg gactatcttc caaagcagct tcctgttttt
      541 gctctaatag ttagattttg cgtaccattt tcttctttta ttttccttcg attgctgatt
      601 ttaatatagt ctaaaggaaa tttatattta ttgtattctt tataacttca agagaacttc
      661 gtttgagtat tactgtattt ttttaattcc ataagaaaat tagttattac ctgccaccta
      721 atcagcaaat ctgaacctga gtcaatataa ttaatattat tttccatctc caacattgta
      781 agtactgttg gtgttaatat agggactttg tacaattgta tttttttgat tttatcatct
      841 gcacaaagga gattttttaa atctaatcct tcttttattt caaaagttga attttttaaa
      901 tctataagag taaaaccaat tccttcatac tgaatttctg aaaatcgaat ttttctccca
      961 gttgatagat caaggggcag aaaacctttt attacattgt gagtgtcacc atcatttaca
     1021 atgtcattag gaattttgat tttagaattc tgtggagcac ctattggaag ataataattc
     1081 attataattt tgtagtcttc atgaggaaat aaagtacttt taggattgct aagatattct
     1141 cgtaccgaat ctaagaatgg cttatccatt gaatcttcat ccccatttaa actaaaaaac
     1201 attcctacaa ttgcttttgc aactctaagt gaattcattt tctttattga atgtgttaaa
     1261 tcagacttac cattctttaa acttgtattg ttataaagga aatttttatt gtcttctgca
     1321 aatctaatat attcctcaac atactcacta ccaaaaaaac tattacattt actacataac
     1381 g
//
LOCUS       DARBER010000234         1375 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DARBER010000234 DARBER010000000
VERSION     DARBER010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.243"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            191..445
                     /locus_tag="SCK06_002807"
     CDS             191..445
                     /locus_tag="SCK06_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1152733.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="SCK06_002808"
     CDS             446..763
                     /locus_tag="SCK06_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1152734.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          420 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DARBER010000235         1362 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARBER010000235 DARBER010000000
VERSION     DARBER010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.244"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            82..>1362
                     /locus_tag="SCK06_002809"
     CDS             82..>1362
                     /locus_tag="SCK06_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG1152735.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DARBER010000236         1341 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARBER010000236 DARBER010000000
VERSION     DARBER010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.246"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1309
                     /locus_tag="SCK06_002810"
     CDS             <1..1309
                     /locus_tag="SCK06_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEG1152736.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          570 a          102 c          227 g          442 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaag a
//
LOCUS       DARBER010000237         1294 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARBER010000237 DARBER010000000
VERSION     DARBER010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.247"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1244
                     /locus_tag="SCK06_002811"
     CDS             <1..1244
                     /locus_tag="SCK06_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG1152737.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DARBER010000238         1287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARBER010000238 DARBER010000000
VERSION     DARBER010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.248"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..848
                     /locus_tag="SCK06_002812"
     CDS             <1..848
                     /locus_tag="SCK06_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1152738.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DARBER010000239         1274 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DARBER010000239 DARBER010000000
VERSION     DARBER010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.249"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..1145
                     /locus_tag="SCK06_002813"
     CDS             6..1145
                     /locus_tag="SCK06_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG1152739.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEAN
                     NQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYTK
                     RKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          463 a          186 c          216 g          409 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gtttaccaag caagttcaaa agaagaagca aataatcaaa
      841 taaggtttat gatagataaa tggaaaaagc agtatccacg agtggttaaa ttgctcatga
      901 atcctgctat attaaccttt tataatttcc ctccgtcaat cagaagaact atttattcaa
      961 ctaatttgat tgaaggtttt aacaaacaat taaagaaata taccaaaaga aaagagcaat
     1021 ttcctaatga agaatctctg gagagatttc tagtatctca attcaacaac tataatcaaa
     1081 agtttctatg ccgagttcat aaaggtttta aagaaataca tgatacatta gaatcaatgt
     1141 tttaacttga tcaacagaat gggcttttcc atttacacaa aattattgac gctcccgaca
     1201 aaattatgat cttgcgtaaa gttataaaat attaataaaa atattttatt aatgaaaagc
     1261 ttggtagcac ggtt
//
LOCUS       DARBER010000240         1254 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DARBER010000240 DARBER010000000
VERSION     DARBER010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.250"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..106
                     /locus_tag="SCK06_002814"
     CDS             <1..106
                     /locus_tag="SCK06_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1152740.1"
                     /translation="IDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            111..1058
                     /locus_tag="SCK06_002815"
     CDS             111..1058
                     /locus_tag="SCK06_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG1152741.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          404 a          182 c          267 g          401 t
ORIGIN      
        1 tattgatagt gaagaagatt ttaaatggtt agaatttttg tttcctaaat ttttagaaga
       61 ttcttcggag cttcaagata tctataatta tttatatcgg aggtaaaagg atgagctgtt
      121 atcttatcac cggtggggct ggcttcattg gttctacctt agccaatcat ttaggagaag
      181 aacatgtggt aatcgtcgtc gatgacttat caatgggaaa agtagaaaat cttgatatga
      241 cgaaaaatat cacttttatt gagggagatg tcgctgagaa atccttgatg gaagagatca
      301 tgaaagctaa taaatttgat tatatctttc atttagcagc agttgctagt gtggcagatt
      361 cagttgcccg accagtagaa acacatcgtg tgaattttga aagtgtatta atgattttag
      421 aattgatccg caagtatcaa cctgatttaa aacggatcgt gtttagttct tcagcggctg
      481 tttatgggga tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc
      541 cttatgcggt tgataaattc gctgcagaac aatatgtgct agattattgt catttatatg
      601 atgtacccgg tagcgcagtt cgttttttca atgtctatgg tccaaatcaa aatccgaatt
      661 ctccatacag tggtgtgatt tctattctcg tagatcgcta taagaaacaa atcgtaggag
      721 aagcaacttc atttacatta tttggtgatg gaagccagtc tagagacttt gtttatatag
      781 atgatgtaat ccaagcatta cttttagttg cgaatgagga agctgcactg gggaaacaat
      841 tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt gattcatgca atcaatgaaa
      901 ttctcggtgt agaattgaca cttgaataca aagaagagcg ttcgggtgat atccatgact
      961 ctctagcaga tattactaag atccagtcaa ttggctatca accgaaatat ggtattttaa
     1021 gtggaatgac taattatttg aaaactgaaa tcaattaaaa catactaaga taagattaga
     1081 gggtaaattc aggctctttg tcaataagga ctgatgagtt ttgtaaaatc aaatctggga
     1141 tagaagggct ctttgtcaaa tgatgttggt aaagagaaat cttagaataa tttggacaga
     1201 acagaaagaa gaaaacctct ttctgttctg tttatttggt taaaaatgtt ttgc
//
LOCUS       DARBER010000241         1235 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARBER010000241 DARBER010000000
VERSION     DARBER010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.251"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..619
                     /locus_tag="SCK06_002816"
     CDS             <1..619
                     /locus_tag="SCK06_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1152742.1"
                     /translation="RNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITL
                     GMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVV
                     QFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSN
                     HTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            764..1069
                     /locus_tag="SCK06_002817"
     CDS             764..1069
                     /locus_tag="SCK06_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152743.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          460 a          134 c          218 g          423 t
ORIGIN      
        1 tcgaaattta gaaaacatgg tttttaatct gagtaatgga ataattccaa gtgttagtgg
       61 agatacgatt cggtctgaaa aaaattattc tatcattgtt tttgaaaagt tagcacaaac
      121 gtctataaca ttaggaatgg atataataga agcgtatcaa agtagagatg ctttgataca
      181 agaaaacgaa ttagctgtat cgttacctga ggtcttaaaa gtgagggatt ctgggattgt
      241 ttattatact aaggaaatcg gaaaaactaa gatagagcat ctttctccct taatttcttc
      301 tgtcgttcaa ttcatcggct taaatatcta taaaagaatt actgtaaagg aaatagctaa
      361 ttatttttct gtaagtgaaa ctaaattaag agaaagcttt aagaatgaaa tgcatattac
      421 gatttataat tatatctcta agcgaaaaat ttctacggct aaaataatgt tgaaatcaaa
      481 ccatactatc agtgaagttt ctttaggttt aggattttca gattcttcac acttttcgag
      541 agtattcaaa aagtacgcag gtgtatctcc taagcaatat caattaggtt tagtggataa
      601 tttcaacaac attagctgag aggttttatt ttattatgag actatatcat ttacgtagtt
      661 tggaggagtt gaggaaattg gattttaaca aaaaattttt tgggaaattt ctgatttgag
      721 ttgtttcgta gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga
      781 caaagtagta attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca
      841 tattaatggt agcaatttta aacaaacatt atttaatctg attaagctag atgctaaaat
      901 atcaagttat ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca
      961 gttaattgaa agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt
     1021 aaacaactac aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta
     1081 gagtactaga cactttaaat atggacaatt caattataaa gaaaaagaat caataattta
     1141 atgaggctgt gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata
     1201 actccaatca ttcgtttaag attctaggaa tcatt
//
LOCUS       DARBER010000242         1208 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DARBER010000242 DARBER010000000
VERSION     DARBER010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.252"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..383
                     /locus_tag="SCK06_002818"
     CDS             <1..383
                     /locus_tag="SCK06_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1152744.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="SCK06_002819"
                     /pseudo
     CDS             complement(367..960)
                     /locus_tag="SCK06_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          366 a          241 c          212 g          389 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttatatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cgggtattaa ttcgagaatc
     1201 acacctat
//
LOCUS       DARBER010000243         1195 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARBER010000243 DARBER010000000
VERSION     DARBER010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.253"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1076
                     /locus_tag="SCK06_002820"
     CDS             <1..1076
                     /locus_tag="SCK06_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1152745.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DARBER010000244         1189 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DARBER010000244 DARBER010000000
VERSION     DARBER010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.254"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..581
                     /locus_tag="SCK06_002821"
     CDS             <1..581
                     /locus_tag="SCK06_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1152746.1"
                     /translation="VAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYR
                     NNYSAFKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRY
                     DDKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEK
                     ELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            582..>1189
                     /locus_tag="SCK06_002822"
     CDS             582..>1189
                     /locus_tag="SCK06_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1152747.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDG"
BASE COUNT          453 a          135 c          208 g          393 t
ORIGIN      
        1 atgttgctgc tccactgagg tataaaaata tcatggaagt tgttcgtaat agtattcaaa
       61 gtaaaaaagt atattctgta agagctattt gttctactta tcttcctgat tggagaaaag
      121 gtactgatta tagaaataat tattctgcct ttaaatctat ggggggagga gtagaattag
      181 actgtataca tgaattggat tatattataa acctatttgg tttccctaaa tcagtaaaaa
      241 aacagtttgg taaatattcg aatcttgaaa ttaacagtaa tgatttagca acatatatat
      301 tgagatacga tgataaaatt atagaaattc atttagatta ttttggaaga gttcctacaa
      361 gaaaatttca attgtttact ttagatgaat gtatcaatgt tgacttatta aataataaaa
      421 taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga
      481 aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat
      541 atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta
      601 tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa
      661 agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac
      721 ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt
      781 taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga
      841 aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat atatttgatt
      901 taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt gaagaattaa
      961 tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt tctgcaagac
     1021 gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata gttaataaat
     1081 caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt
     1141 cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggca
//
LOCUS       DARBER010000245         1183 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DARBER010000245 DARBER010000000
VERSION     DARBER010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.255"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..153
                     /locus_tag="SCK06_002823"
     CDS             <1..153
                     /locus_tag="SCK06_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1152748.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="SCK06_002824"
     CDS             159..575
                     /locus_tag="SCK06_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152749.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="SCK06_002825"
     CDS             681..944
                     /locus_tag="SCK06_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1152750.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="SCK06_002826"
     CDS             944..>1183
                     /locus_tag="SCK06_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1152751.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DARBER010000246         1172 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DARBER010000246 DARBER010000000
VERSION     DARBER010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.256"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(242..1090)
                     /locus_tag="SCK06_002827"
     CDS             complement(242..1090)
                     /locus_tag="SCK06_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1152752.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          374 a          196 c          204 g          398 t
ORIGIN      
        1 caaaaataat aaaaaaaaga aaagataaat tttttactac tgtatactaa ctttgttatc
       61 gcatctaaca agtaactcga ataataccaa tacaaccttc tccaaagatg cgatgacata
      121 cgttctaacc caccgactat tgatgttctg taccaccctt gcagaaccaa aaaagccact
      181 accaaatatt tggtagtggc tttttttgtt ttatggattt ttcgcttttt ctgttatggt
      241 gctattcttt gatttcagaa atagtatgtc gaactttagc gatagaacga cgtgattctt
      301 tttcgttgta aaacatcaaa atcacatata gagagtccaa aatgcttagt tgagaaatac
      361 gggaagctaa agcttcagat cggaattctg tttcttcaga aatagaaata aaaacgacat
      421 cgccaagttt agcaagggaa gaattagctt ggctagtaat aacgattgtt tttgctccag
      481 tttttcttac ggtttcagca atatggattg tttcttttga ttgccctgtg tgagaaatca
      541 aaacagcaca gtctttttcc gtcaataaag aagcttccat caattgaata tggtaatccg
      601 tgctgtgaga agttgcgata ggggaacgca aaaatttatg ataagcgtca gttgctacaa
      661 tctctgaacc gccaacaccg aaaaaataga cacgtttaga ttcattgata atagtagccg
      721 ctttttttag atccttttgt tgtaacaact tttttgtgtc atttaaagtt gaaatactag
      781 actcgaacac cttttgcgcc atagtgagct cagtatctgt tttctgaata ttttcgtgga
      841 tagaaatagc agatagatca ttttcttgga tcaacatggc cattttaaaa tctttaaatc
      901 cattataacc aagttttttt gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac
      961 ttgctaaatt gctaatagga ctatgactta ctttgcttgg attttctaaa atataatcgg
     1021 cgatagcctg ttcttttggg ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg
     1081 agacgctcat gagaattcct cattttcata ataagattat cattttttcc gttctcatta
     1141 atgttaaaga tacaaggaga gtaggaaaaa at
//
LOCUS       DARBER010000247         1163 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DARBER010000247 DARBER010000000
VERSION     DARBER010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.257"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            79..489
                     /gene="spx"
                     /locus_tag="SCK06_002828"
     CDS             79..489
                     /gene="spx"
                     /locus_tag="SCK06_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1152753.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            503..859
                     /locus_tag="SCK06_002829"
     CDS             503..859
                     /locus_tag="SCK06_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG1152754.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          412 a          165 c          219 g          367 t
ORIGIN      
        1 tatatattat tagctaataa ttggtatttt aaaagaaatt actgtagaat tggattataa
       61 ttaaaaatga gtgagaaaat gataaaagta tatacttcgc ctagctgtac ttcctgtaga
      121 aaagcaagag catggttgca agcgaatcaa cttgaatttg aagagaagaa tatttttgcc
      181 gaaccgttaa cagaaagtga gatcaaaaag atcttggcag cgactgaagg aggagttcaa
      241 gatattattt ccactcggtc gaaaatttac gaaaagctgg gcatagattt taacgaatta
      301 actttaaagc aaatgatcgt cttattcaaa gaatatcctt cattattgag acgacctatt
      361 ttgattgatg aaaaaagaat actaatcgga tttaatgaag atgagatccg gtcatttgtc
      421 cctcgtgaaa ttcgatgtgt tgccagaaat ctgatctatt tacaaaacaa taacatctta
      481 aattcatagg gagggagcct tcgtggaagt gaaagtctat ggatcagcgc actgtgtaac
      541 aagtctgaag gcgaaacaat ggttagaaca ccatcaattg ccttatcaat ttgttgactt
      601 agaagaacgc gatctaagta cagaagaagc agaagaatta tgcagttttg aagaagtgca
      661 agcagaacag ctatttgcga cttggtctga ggcttttcac aacattgcga ttgacctttc
      721 ctgtgtagga aaaaaacaac tgattttttt atgcacacaa cataccaatc tcctcagacg
      781 tcctttgatt attatcgatg aagttctatt tattggatac aatgagcagt tgatggaaca
      841 gcttatctta aataaataga ctttaactgt tttaatcaag tcactgagca tttcatacag
      901 ttgtaacagt cgaacactta gcatcaagcg tgttatgtgt tctttttttg actttatttt
      961 tctactatag agacatgctc aatttatgtg gtcaaaattc aaatctaggt ggggatatag
     1021 agttaaaaat atattgacat gtgtaaattt acatgatata ttcatttctg ttgttttaag
     1081 tagcaaagca ataaaatagt attttaaaaa acatataaaa tagttgttga catgttagaa
     1141 atgacatgat aagataactg agt
//
LOCUS       DARBER010000248         1161 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DARBER010000248 DARBER010000000
VERSION     DARBER010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.258"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(257..1129)
                     /locus_tag="SCK06_002830"
     CDS             complement(257..1129)
                     /locus_tag="SCK06_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1152755.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          317 a          277 c          225 g          342 t
ORIGIN      
        1 atttgagtgt aacataaatg gcttttttat ttgtctcgct taattataca aacggcgtgt
       61 aaaaaactat tgcacaatac aagtggccta cggctatcga aatttccata actttcgagc
      121 aagaatttat ctcattcaag gcttgatctt ccaagtaaaa caaaaaccag ttaagcactc
      181 tgcctaactg gttatgacta aattttcagt tgtatctttc tctaccaaca ctaaatgtcg
      241 aagagccttt tttcgtttat gaatgtaaga ccgcttctcg agccaatgcc aaacaaccga
      301 gcgttccagc gttgtcttct aatgctgggg tcacgatata tttgcttaac tcaggtgttt
      361 cgacataccc attgaccaat tttgcaaatg cgtcgtggac gttttgcagc atatggcgtt
      421 gtttcatcac accaccgcca aaaatgatca catctggaga aaacatcaag gttgtattgt
      481 aggcacattg agcaatatag gaggcttcga tttcccaata aggatcatct tctgcaacct
      541 cttgtccttt tttccctaag cgtttttcga tggcgggtcc agcggccatc ccttctaaac
      601 agttgccatg aaatggacag ctgccagcaa agtcatcttt tggatgaggg acgacgagca
      661 tatgccccat ttctggatga ctaaaccctt cgataaagcg cccttcttgt aaggcaccgc
      721 cgccaacacc cgtaccgatc gtatagtaaa cgacactgct caatcctttg ccactgccga
      781 agacatactc accataggca gccgcattga catctgttgt ccaagaaatc gggatcggaa
      841 attctttttt catggtaccg atgaaatcaa aattttgcca tgccaatttc ggggtcgagg
      901 taatgtaccc ataagtggcg gaatcgcgat ggatatcgat aggcccaaat gagccaatcc
      961 caatccctgc taaatctgct tggtattgtt tgaagaagtc aatgactaaa ggcattgttt
     1021 cctcaggtgt cgttgtcgga aagctcactc tttccttgat cgtcatttct tcatcgccaa
     1081 tggcacacac aaattttgtg ccgcctgctt caattgcacc gtatagcata gacatttctc
     1141 cctttatttt agaatgaaat c
//
LOCUS       DARBER010000249         1143 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DARBER010000249 DARBER010000000
VERSION     DARBER010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.259"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="SCK06_002831"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="SCK06_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG1152756.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DARBER010000250         1116 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DARBER010000250 DARBER010000000
VERSION     DARBER010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.261"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SCK06_002832"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SCK06_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG1152757.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DARBER010000251         1114 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DARBER010000251 DARBER010000000
VERSION     DARBER010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.262"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..256)
                     /locus_tag="SCK06_002833"
     CDS             complement(<1..256)
                     /locus_tag="SCK06_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1152758.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKN"
     gene            complement(253..906)
                     /locus_tag="SCK06_002834"
     CDS             complement(253..906)
                     /locus_tag="SCK06_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1152759.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          351 a          190 c          103 g          470 t
ORIGIN      
        1 tatttttttc ttttatgaat acttttattt cactactctt ggaataaaac tccaaagttt
       61 gttctatata ttttatttct tttccattca tattactaga aatttcattt gccatattat
      121 gaatcttttc ttttctcgtc tccaaatatg ttttaggaaa gataaaaaaa attaatgaat
      181 gaactaatat aattattatt ccaagaacag aaaatatttg tatgaacatt tttggaaata
      241 tctttaattt tttcattttc tctccaattt atatcctaca tttcttatag tagttataca
      301 gtctaattgt aatttttttc taagttcttt tatatataca tctattactc gatcataagg
      361 agtttcttcg ctatctttcc atacataatc aataatttgc attcttgtta acacttgtcc
      421 attattatcc aataaacatt ttaatacttc tagttctttt gcatttacat ctatttcttc
      481 accatttatt ttagctgtat agctattaaa attaaccgat agatccttat attcaaactt
      541 ctctaaatgt ccataattct tcttaattaa agaatctatt ctagctttta agaccggcaa
      601 tgaaaatggc ttttctacat atccgtctac taaattagta aatgcatcaa ttttatattc
      661 ttcatcacta aatgcagtca agattagaat tggtaaattg cttttctttc taatttcttt
      721 caacacttct aaaccattta taaaaggtat ctgaatgtct aagataacca aatttatatc
      781 gctattaaat tttgacaagg cttctcttcc atctttagct tgaataacag tatatccaaa
      841 ttctgaaaga tattcactta ttccctctct tatcatatta tcatcttcaa cagttaatat
      901 tttcatatat acctccactt aagcctatac actagatatt atacagcata ttatggctct
      961 ataatatcta gtgttggtga caccttctgt tgccaatact ttttcttttt tctaataaat
     1021 gcaaacagac accttgaaat cctttagaat aaagggttct ataatatttt gtgttgttgg
     1081 aaaatcgaag gcgattttct actaacactt tttc
//
LOCUS       DARBER010000252         1106 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DARBER010000252 DARBER010000000
VERSION     DARBER010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.263"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1087
                     /locus_tag="SCK06_002835"
     CDS             <1..1087
                     /locus_tag="SCK06_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1152760.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DARBER010000253         1089 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DARBER010000253 DARBER010000000
VERSION     DARBER010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.264"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            66..851
                     /locus_tag="SCK06_002836"
     CDS             66..851
                     /locus_tag="SCK06_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG1152761.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          427 a          163 c          177 g          322 t
ORIGIN      
        1 cccttttaga aaaagcaaaa aaaaaagaaa aaatcttatt cttgataaca agctacgtga
       61 cgtatatgcc taacaaaaat agaacttttg aatcaatctt aaatgaaggt ttaaccacag
      121 aaccggcatc cattattttt gatgaagtta tcgatgacgt tgaagatagt aagtatcata
      181 aaaatttaaa agaacttttt tattttgcac tcaacaatgg accaagtttg ttcactggta
      241 tatcagtacc tgatagtgat aatccattaa ttatggcaaa aatttactta acaaaatggt
      301 gtaataaata catcagggat aggaataacc cagcattaaa aaaacctctt aaaacatttg
      361 gcgaaaaaga tgctgcttta actactagag tagcagcaaa tgctaatatt gatgatcaaa
      421 aagtattgaa tgactacttg cgtggacatt ttttatatat gagcgctgag aacatgaacg
      481 gaagtatttt agaggaatat ttagccgaag tgttagaacc tgaaggttgg atttggtgcg
      541 ccggctctgt atatcgagct gttgatttct gttatttagg aacctcacca attcttttac
      601 aagtgaaaaa taaatacaat acagaaagca gttcgagttc agctataaga gtaggaacaa
      661 caattagaaa atggaatcgt ttaaataagt cgacaaaaat ttctggtttg gatagcccta
      721 tccccaattg gaaagcatta atagaaatga ctgaagctag taaggaactg gcagctaagt
      781 taactgaaaa tagttattta gcttatatta atgaaaaatc cacacgagaa ttatggacac
      841 tcgatgacta acaatatcct tattagtatc tatccacgat acatggaaaa aattataaac
      901 aaacaaaaaa actttgaatt tagaaatttc tatcttacct cagatcctta taatatcttt
      961 tggatttacg aaacaatccc caccaaagct ttaaagtata aattgattgt aaaaaatcca
     1021 attactaaac ttaagaaaaa tcaacactac tttttaggag acgatgagtt tgaacaatta
     1081 gtttaaaag
//
LOCUS       DARBER010000254         1051 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DARBER010000254 DARBER010000000
VERSION     DARBER010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.265"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="SCK06_002837"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="SCK06_002837"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEG1152762.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DARBER010000255         1035 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DARBER010000255 DARBER010000000
VERSION     DARBER010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.266"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="SCK06_002838"
     rRNA            complement(<1..>1035)
                     /locus_tag="SCK06_002838"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DARBER010000256         1023 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DARBER010000256 DARBER010000000
VERSION     DARBER010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.268"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            392..1023
                     /locus_tag="SCK06_002839"
                     /pseudo
     CDS             392..1023
                     /locus_tag="SCK06_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          350 a          182 c          186 g          305 t
ORIGIN      
        1 ttttttatat tacaaaagta gtgtttcaaa gttagtagag aaaatgtagt tatatattta
       61 aatttttacc agaaagaaat gaacaccttc aattatctaa atctcgaaaa tggttagatg
      121 taatcatcag gaaaaacaat cttaaacgaa ttactattca tggtcttaga cacacacatg
      181 ctagcttgct tttagcaagc tagtgcaaat attaaagatg tgcaagagcg tttaggccac
      241 tcttctattc aaatcattat ggatctatat attcatatta cagacaaaag aaaagaagaa
      301 accgtagcgc aattcgcaaa atataggctc tataatttat gggactgatg aatcaaaatt
      361 gattcttcag tcccattgtt attttaggta tatgattcgt acattgaaag agttgaaaaa
      421 ctatcttccg aagccgtcaa aaaggaccct gccaaaagta ttgatggtgg atgaatttcg
      481 ttcgcacgct tcaattgtgg ataaaatgag ctttatttgt gcggatggcg agactggaaa
      541 actcattgat attttgccta cacgaaaatt gtctcgatta acacactatt ttagtcgctg
      601 tgcaaatcct gaagcggtgg aatatttagt gacagatatg aacgccgcct acttccaact
      661 gacaaaacgt gtcttaccaa atgcgaaagt cgtcattgat tgttttcata tcatcaaaca
      721 tttgaatcag gcgttcaatg attttcgtgt acaagaaatg aaaagattac accagttagg
      781 gcaacgacgg gaagccgaga aagtgaaaaa gaactggcgc tttcttctaa aaaaccgtca
      841 aaaaatcaat cactatgatt acaaaacatg gcgcagtttt cgtgcaccta aatacccgct
      901 actcactgaa gcaatgatga ttgatcgatt gctggcattt tcaacgcctt taaaggacgc
      961 ctatactttt ttccatgagc tgaccgaagc ctttagagac aaggaatcag acttattttt
     1021 tac
//
LOCUS       DARBER010000257         1015 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DARBER010000257 DARBER010000000
VERSION     DARBER010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.269"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SCK06_002840"
     CDS             <1..704
                     /locus_tag="SCK06_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1152763.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          344 a          163 c          169 g          339 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataagg gttctgttgc aaagttttaa ataaa
//
LOCUS       DARBER010000258          998 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DARBER010000258 DARBER010000000
VERSION     DARBER010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.270"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>998
                     /locus_tag="SCK06_002841"
     CDS             <1..>998
                     /locus_tag="SCK06_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152764.1"
                     /translation="FSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESKSFDDN
                     IGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACLYRNNK
                     NVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGRE
                     NTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKI
                     ADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGS
                     VISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAE"
BASE COUNT          383 a          114 c          179 g          322 t
ORIGIN      
        1 ttttttccga tgagtatgtt gaaattatta tttctgatca ttctaaaaac actaaagaaa
       61 ttgcgaaaaa aatagaagaa aaaaaagtgt ttgatggctg tcattattta gaaagcaaat
      121 cttttgatga taatattggg tattttcaaa agattaagga ctgtcttcag atttcttgtg
      181 gtaaatcaaa tcgatatgcc tattatttga aaaactgtaa aagtgaatat tttgatgaat
      241 ttatttgttt ttcaatggga gaggtttggt tggaaggaat atatgcttgc ttatatcgaa
      301 ataataaaaa tgtacaggta tcaattttag aagaaggtat tttaagctat ggagttgaca
      361 ttattccaac agcaagaaca aaagctatcg cagtactcaa ttacttaaaa aaaaggactc
      421 cactatataa caaaagctat aatttttatt gtttttttcc taatatatat acaggaagag
      481 agaatacaat acaaattcca ttagttacag ctgggtcaag aaccgcattg caactttcgg
      541 atatttttaa ccctatgata gatatttata aagaaaaaaa atatatattt tttacgtctg
      601 tttatgattt tgagggcgga gaacctgtcg gagaatatga attagtatgc aaaatagcag
      661 acttagtagg aaaagataat ctaattgtta agacgcatcc tagagatgta agaacaattt
      721 ataaagataa tggatttatc gaggataaaa attcatcaat accttgggag gcgattcaat
      781 tgtcaggcga ttttaacgat aaaatattta tgacaattaa ttcaggaagt gttatttcag
      841 gtagtacaat gtctgcaaaa cctgtacgca cgtattatat gtataaattg tgtaacatag
      901 aaggaaatcc gtcttgcaaa aagaatgcgt atgatattga gcgattgctt aagcaaaaaa
      961 cgatgaaaaa cattttaaag aatgtgaaaa tagcagaa
//
LOCUS       DARBER010000259          995 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DARBER010000259 DARBER010000000
VERSION     DARBER010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.271"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..913)
                     /locus_tag="SCK06_002842"
     CDS             complement(26..913)
                     /locus_tag="SCK06_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG1152765.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DARBER010000260          960 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DARBER010000260 DARBER010000000
VERSION     DARBER010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.272"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..>960)
                     /locus_tag="SCK06_002843"
     rRNA            complement(1..>960)
                     /locus_tag="SCK06_002843"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          213 a          298 c          209 g          240 t
ORIGIN      
        1 taagtcctcg atcgattagt atcagtccgc tccatacatc actgtacttc cactcctgac
       61 ctatctacct gatcatctct cagggatctt actttcttaa agaaatggga aatctcatct
      121 tgaggtgggc ttcacactta gatgctttca gcgtttatcc cttccctaca tagctaccca
      181 gcaatgccct tggcagaaca actggtacac cagcggtaag tccatcccgg tcctctcgta
      241 ctaaggacag ctcctctcaa atttccaacg cccgcgacgg atagggaccg aactgtctca
      301 cgacgttctg aacccagctc gcgtgccgct ttaatgggcg aacagcccaa cccttgggac
      361 cgactacagc cccaggatgc gacgagccga catcgaggtg ccaaacctcc ccgtcgatgt
      421 ggactcttgg gggagataag cctgttatcc ccagggtagc ttttatccgt tgagcgatgg
      481 cccttccatg cggaaccacc ggatcactaa gcccgacttt cgtccctgct cgacttgttg
      541 gtctcgcagt caagctccct tctgccttta cactcttcga atgatttcca accattctga
      601 gggaaccttt gggcgcctcc gttacctttt aggaggcgac cgccccagtc aaactgccca
      661 tctgacactg tctcccacca cgattagtgg tgcgggttag agtggccata acgcaggggt
      721 agtatcccac cagcgcctcc atcgaaacta gcgttccgat ttctacggct cctacctatc
      781 ctgtacatgc ggtacagaca ctcaatatca aactacagta aagctccatg gggtctttcc
      841 gtcctgtcgc gggtaacctg catcttcaca ggtactaaaa tttcaccgag tctctcgttg
      901 agacagtgcc caaatcgtta cgcctttcgt gcgggtcgga acttacccga caaggaattt
//
LOCUS       DARBER010000261          959 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DARBER010000261 DARBER010000000
VERSION     DARBER010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.273"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..511)
                     /locus_tag="SCK06_002844"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="SCK06_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="SCK06_002845"
     CDS             complement(570..>959)
                     /locus_tag="SCK06_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152766.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DARBER010000262          950 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DARBER010000262 DARBER010000000
VERSION     DARBER010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.274"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="SCK06_002846"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="SCK06_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1152767.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          279 a          195 c          142 g          334 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaacc aattatgaat tgacaaataa aacctgtttt
//
LOCUS       DARBER010000263          936 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DARBER010000263 DARBER010000000
VERSION     DARBER010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.275"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..481
                     /locus_tag="SCK06_002847"
                     /pseudo
     CDS             1..481
                     /locus_tag="SCK06_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            515..>936
                     /locus_tag="SCK06_002848"
     CDS             515..>936
                     /locus_tag="SCK06_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HEG1152768.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKK"
BASE COUNT          342 a          104 c          176 g          314 t
ORIGIN      
        1 tgaacttttt caaaatcaca tttcaattga agaaagcatg agtcaattaa gagatagaat
       61 acttaaagag tgtgcaggtt cttctgagta taattattta gaaagtgacg aatttatagc
      121 attaagaaac tatgttgtta ataaaatatc accaattatt gattcgacag gtctatgttt
      181 attagatgaa ttcttggcgg atatcagtct atttataatg tttattgatg taattgaaga
      241 aacatcaaga aattttttgc tgtctaagtt cagaaaattt cgtatgaaaa atgataaaca
      301 aaaattattg gcaaatgaga tttggagtag tttagaagtc gaagaaagta aacaagaact
      361 tttgttgaaa tggatacttt ttcttgtaaa gaagttctat gtgaaaatac cagaaaccca
      421 ttgtctgata gttatgcacg gtaaaataac agcttctgcg atcgcaaggg aaacaaataa
      481 gttgctgaat acatatgttt atgaggcttt cgatatgcca attgaaggtg agactagtga
      541 cttaattaac ctaatcaaca atttttgtaa aagtatagat acgacgaatg gactcattct
      601 gctagttgat atgggatcat tggaacaaat gtatgaaaaa attgaatcca atgtaatagg
      661 tgatttggta attttaaaca atgtttctac agctttagcg attgaatgtg ggattcaagt
      721 ttgccaaaaa aagccaatta gtcattttta ccagatggat tttgattcgt ttcaagttaa
      781 agttcagtat tataaaggat tatcacaaaa gaaaaatgta atagtatctt gtctctctgg
      841 tgaggggatt tctgaaaaag ttaaggatat tttaaaacgg tatctcgata atcaagtgga
      901 gattttgatt tttgatttta acgcattaaa gaaaat
//
LOCUS       DARBER010000264          931 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DARBER010000264 DARBER010000000
VERSION     DARBER010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.276"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>931
                     /locus_tag="SCK06_002849"
     CDS             <1..>931
                     /locus_tag="SCK06_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1152769.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DARBER010000265          925 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DARBER010000265 DARBER010000000
VERSION     DARBER010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.277"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            96..>925
                     /locus_tag="SCK06_002850"
     CDS             96..>925
                     /locus_tag="SCK06_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1152770.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          319 a          136 c          160 g          310 t
ORIGIN      
        1 attttgtgta aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag
       61 ttaccacaac cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta
      121 tggaaacact aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag
      181 ctgttaattc attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc
      241 gtgctgggtt caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag
      301 agtatggaga attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa
      361 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc
      421 aaaaaggaat tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact
      481 atacgccaca aaccatttca aatatgacta aaatagtttc tgaagatatt attgccttta
      541 aagaaaggtc cttagaatcc agatactctg tcatttttat ggatgctact cacattcctt
      601 taaaaagaca aactgtttca aaagaagcag tttatattgt aattggcatt cgattagatg
      661 gaaccaaaga ggttcttggg tttagtcttg ctccaactga atctgcttat gtttggaaag
      721 agattcttca agacctaaaa gaccgtggtt taaaagaggt tttattagtc gtaactgatg
      781 gtttaagtgg cattaacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      841 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      901 tctgtagcga tttcaaatta gttta
//
LOCUS       DARBER010000266          923 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DARBER010000266 DARBER010000000
VERSION     DARBER010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.278"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            55..747
                     /locus_tag="SCK06_002851"
     CDS             55..747
                     /locus_tag="SCK06_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1152771.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          414 a          107 c          137 g          265 t
ORIGIN      
        1 gaaaataatt tttaataact tatttaagga aattttttga aggagaagat aaaagtgaga
       61 aaaaagatta ttattaatgc tggagtaatt ataggattga tgttagtaat tttctccgga
      121 aagcaatatg ctgataatat tttattatgg ggaggggaag ataatattga aaagatcaac
      181 aacaatttaa ctatattaga cacagctcta gaaaataaag agcaaaaaat tagtatttta
      241 aattcacaat tgtcttcaaa caaccaaact cttgaacaac taagaactaa tattgaagtt
      301 tccaaacaaa aagtggcatc tttagaaaac gacaagaatc aattagtatc agagaaaact
      361 aatttagaaa atcaattgtc agcaaaagac tctgaattgc aaggaaaaca aaatgaaatt
      421 aatgcgaaaa tagacgaaat taaccaaaaa gtagctgaaa taaatcgatt aaatagcgat
      481 tggaataatc gtttacaaaa tgaaaagaac aaatttaatg aagaacaatc tactattaat
      541 agtttaaacc agcaaattaa tcaaatgttg agtgaaaaaa ataacattca aaatcaactg
      601 caagctactc agcaaaagct agaagaagaa aagtcaaaaa atagtagttt gaataattac
      661 attgaaaagc ttcaaaaagc taaaaatgat gtagaggata ccgcaaataa agcacaacaa
      721 attgtaaatg aacatacaat ccaatagata tagttttaat ttaacaaatg gtatgtttgg
      781 agataatgag atagaatata aatgtcataa tattatacaa tctaaacacc gtttcctttt
      841 ctatttttga tgacatcagt atatagctac tcgaaagagt agtcttttta ttattctcgc
      901 ttattattga tatgaaaatt tcg
//
LOCUS       DARBER010000267          904 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DARBER010000267 DARBER010000000
VERSION     DARBER010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.279"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            265..888
                     /locus_tag="SCK06_002852"
     CDS             265..888
                     /locus_tag="SCK06_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1152772.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DARBER010000268          882 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DARBER010000268 DARBER010000000
VERSION     DARBER010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.280"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            87..356
                     /locus_tag="SCK06_002853"
     CDS             87..356
                     /locus_tag="SCK06_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1152773.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="SCK06_002854"
     CDS             349..606
                     /locus_tag="SCK06_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1152774.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          336 a           97 c          168 g          281 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgcttata ta
//
LOCUS       DARBER010000269          881 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DARBER010000269 DARBER010000000
VERSION     DARBER010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.281"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>881)
                     /locus_tag="SCK06_002855"
     CDS             complement(<1..>881)
                     /locus_tag="SCK06_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1152775.1"
                     /translation="SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHD
                     CGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREAT
                     KHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL
                     HFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMN
                     RFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYN
                     EELRTAYHFCQLLRYYF"
BASE COUNT          250 a          169 c          192 g          270 t
ORIGIN      
        1 gaaataatag cgtaataatt gacaaaaatg atacgcagtt cgcaattctt cgttgtagct
       61 taacagctca tcaacgattt ctgtttgact aattggtcgt ttaaacagtc gatgataatg
      121 ccgtttgagt ggctcccatg tcgtggaatc tttgagtaag agtttccaat atcgcttgag
      181 ccgacggtac ttcttctgat tttcagagtg tgaggttcga aaacgattca tgatctggac
      241 acgtaattga ttgaagctgc gattgatgtg ttggacaata tgaaagcggt cagttacgat
      301 ctccgcattt ggaaagactg ttttaagcaa ctgacaatag ctggcattca tgtccatcac
      361 taggtacttg acctttaaac gggcttttct aggaaagtgc aggaagtatt gagttagttt
      421 gtaaagtctg cgatcttcaa ggatcgtaaa tagctgatgg gttgtcccat caacacaaat
      481 aaaactcatg gaaccagaaa cagacttcat tgatttgaac tcatccatac acaacaccgt
      541 cggaaggaag ttcatacgtg actggtaatg cttcgttgct tcacgcaaga tccgtgtcac
      601 cgtcccatcc gagacgaagt acttcttagc gattagttta cgtgattgat tttcgtacag
      661 ttccatgaga attgcctgct tcagttcctt tgttagttga tgatgttcat caactaagtc
      721 cgttttcgca gaaaaggtcc ttccacaatc atggcagaga aaacgggtac gttttaattc
      781 taaaatagtt aacttttctt tgactttcaa tatctgagtg cgtgtcgtat aggaaccgtt
      841 gcggataata cgaaccgatc gacagtgttc acaataggaa g
//
LOCUS       DARBER010000270          870 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DARBER010000270 DARBER010000000
VERSION     DARBER010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.282"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            39..656
                     /locus_tag="SCK06_002856"
     CDS             39..656
                     /locus_tag="SCK06_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152776.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          332 a          111 c          155 g          272 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt
//
LOCUS       DARBER010000271          846 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DARBER010000271 DARBER010000000
VERSION     DARBER010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.283"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>846)
                     /locus_tag="SCK06_002857"
     CDS             complement(<1..>846)
                     /locus_tag="SCK06_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG1152777.1"
                     /translation="ISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLV
                     LFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRK
                     VEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDF
                     YGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFS
                     LLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLY
                     LKKEN"
BASE COUNT          327 a          145 c           99 g          275 t
ORIGIN      
        1 aattctcctt ttttaaatac aagtaagaaa tgcttcccaa ataataccag aaaacaattg
       61 aaatgacatc gcgcttattg aaaagtacat gagcctccac catttcatct ataaaaatag
      121 atagtataaa aattaaaaca ataccaagaa acgcataatt cactttactt ctaaaaaatg
      181 ttttgagtaa actaaagata tttaacacat agaaagcata gatacatata aaagcaatta
      241 taccacttga tacaagtact tgaagaaatc cattatgcat gtttccatgt gctcttttta
      301 gttcattcac atctgtagta tcaagatcta cttttttcaa aaattttgca tcgctattcc
      361 catagaaatc ggtctctcca taaccaaata acaaagttgg tcttataact ttaagcgcag
      421 ctttccaaat cgtaaaacga ccagctgaaa tttcggagtt atcactagaa tgctgtaata
      481 ttacttcttt ttctggaagt ggagttttat ctgattcatt tgactctata acttctactt
      541 ttctatcaat cgatacagga attgttgcta aaaaatcctt agcataatta gtaccaaaag
      601 aaaaatatac caatagaact aataatatgc ttaccatcca tttgattttt acaggtagta
      661 cttctctaat gctctttcta aattttgaat tggcaaacaa caaaagaaat aatactaaaa
      721 aagaacaaat tactacctgg gcacctctag acgaagaaag tgtaatataa aatatttgta
      781 cgataaaatt catcatataa aaaacacgaa tcgtttttgt tatttttgaa attaatacac
      841 taatta
//
LOCUS       DARBER010000272          842 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DARBER010000272 DARBER010000000
VERSION     DARBER010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.284"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(29..101)
                     /locus_tag="SCK06_002858"
     tRNA            complement(29..101)
                     /locus_tag="SCK06_002858"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:His,seq:gtg)
     gene            complement(104..177)
                     /locus_tag="SCK06_002859"
     tRNA            complement(104..177)
                     /locus_tag="SCK06_002859"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(143..145),aa:Trp,seq:cca)
     gene            complement(184..264)
                     /locus_tag="SCK06_002860"
     tRNA            complement(184..264)
                     /locus_tag="SCK06_002860"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Tyr,seq:gta)
     gene            complement(266..341)
                     /locus_tag="SCK06_002861"
     tRNA            complement(266..341)
                     /locus_tag="SCK06_002861"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Phe,seq:gaa)
     gene            complement(377..449)
                     /locus_tag="SCK06_002862"
     tRNA            complement(377..449)
                     /locus_tag="SCK06_002862"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(414..416),aa:Asp,seq:gtc)
     gene            complement(459..531)
                     /locus_tag="SCK06_002863"
     tRNA            complement(459..531)
                     /locus_tag="SCK06_002863"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(496..498),aa:Val,seq:tac)
     gene            complement(547..618)
                     /locus_tag="SCK06_002864"
     tRNA            complement(547..618)
                     /locus_tag="SCK06_002864"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Glu,seq:ttc)
     gene            complement(633..722)
                     /locus_tag="SCK06_002865"
     tRNA            complement(633..722)
                     /locus_tag="SCK06_002865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(686..688),aa:Ser,seq:gga)
     gene            complement(762..835)
                     /locus_tag="SCK06_002866"
     tRNA            complement(762..835)
                     /locus_tag="SCK06_002866"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(799..801),aa:Asn,seq:gtt)
BASE COUNT          236 a          231 c          192 g          183 t
ORIGIN      
        1 cgcttggcta taccccaaga ataaaaaggg cgaatgatgg gaatcgaacc cacgaatgcc
       61 ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatggcagg ggcagcagga
      121 attgaaccca cactaacggt tttggagacc gctgttctac ctttaaacta tgcccctata
      181 aaatggagga gagtggattc gaaccactga accctaagga acggatttac agtccgtcgc
      241 gtttagccac ttcgctactc ctccatggtg gcgcgggaca gaatcgaact gccgacacac
      301 ggagcttcaa tccgttgctc taccaactga gctaccgcgc catattttaa aattattaat
      361 tgaataacaa gaacgacggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc
      421 aggcatgtta acccctacac cacggaacca gttcattatg gaggttaacg ggatcgaacc
      481 gctgaccctc tgcttgtaag gcagatgctc tcccagctga gctaaacctc caacattcaa
      541 ttaaaatgac ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa
      601 ccgcttgacc aacgggccag gtaaatttgc tacggagagt aagggattcg aacccttgag
      661 acagtttggc accatctaca tgatttccaa tcatgctcct tcggcctctc ggacaactct
      721 ccagagtgaa agaagctcaa tactctttct caaaatgaga actccggcag taggactcga
      781 acctacgaca tcatgattaa cagtcatgcg ctactaccaa ctgagctatg ccggaataac
      841 at
//
LOCUS       DARBER010000273          823 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DARBER010000273 DARBER010000000
VERSION     DARBER010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.285"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..>823
                     /locus_tag="SCK06_002867"
     CDS             4..>823
                     /locus_tag="SCK06_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152778.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSE"
BASE COUNT          263 a          109 c          119 g          332 t
ORIGIN      
        1 tatatgaatt tgaaaaaaat tttttctgta aaaatattta tagtttatgc atttgttagt
       61 ttattatatt tatttccagc atttaaaaac atggaaatat ttggctttta tgatacggca
      121 tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat taattttgaa
      181 acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac tctatatcct
      241 ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt tctttatatt
      301 gttacgctca ttactctatt tatatgtcat tatgtaatgt atgaaattac caaaaaacat
      361 gttactagct cctttttcgc tattatatat acaacatctt cttttaggag tgtagaaata
      421 tttcttcgag gggcaatggg ggaattactc gcaatgtcaa tacttcctct tattttactc
      481 gggtttataa agctttacga ctcaaaaaaa gaatcttggg ttatgttggc gattagcatg
      541 actttattaa tttacactca tgttctttct gtagctatga ctatggtaat gattataatt
      601 gctttaatta ttcaatttta tcggaaaaag aaaatagaaa tcttcttaat tattaaatta
      661 ataaaagctg ctatagttac tttacttctt tcactagcgt ttataggtcc tatgattgag
      721 cagacattgt atcaagatct gaatagacct tatgtggata tattacaaaa aagagcaatt
      781 tatctgggta aagagttttt gattggaagt atagattcag aat
//
LOCUS       DARBER010000274          811 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DARBER010000274 DARBER010000000
VERSION     DARBER010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.286"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..672
                     /locus_tag="SCK06_002868"
     CDS             <1..672
                     /locus_tag="SCK06_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152779.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DARBER010000275          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DARBER010000275 DARBER010000000
VERSION     DARBER010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.287"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SCK06_002869"
     rRNA            <1..>733
                     /locus_tag="SCK06_002869"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARBER010000276          712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DARBER010000276 DARBER010000000
VERSION     DARBER010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.288"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..446)
                     /locus_tag="SCK06_002870"
     CDS             complement(<1..446)
                     /locus_tag="SCK06_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152780.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DARBER010000277          707 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DARBER010000277 DARBER010000000
VERSION     DARBER010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.289"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..656
                     /locus_tag="SCK06_002871"
     CDS             33..656
                     /locus_tag="SCK06_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152781.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          247 a          109 c          151 g          200 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatc
//
LOCUS       DARBER010000278          703 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DARBER010000278 DARBER010000000
VERSION     DARBER010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.290"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          297 a          106 c          122 g          178 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgt
//
LOCUS       DARBER010000279          691 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DARBER010000279 DARBER010000000
VERSION     DARBER010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.291"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          232 a          116 c           94 g          249 t
ORIGIN      
        1 ggtatgtcct atgatgaaaa gattgtacta ttcattgatc ataacgattg gctatttgat
       61 tgtatcaaat ctaggaaata tggtatttgg tatcagtaaa gaattcagtt ggactactac
      121 attatgggaa tctctattct ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa
      181 ataaacatgt gccatctatc tgttggcaca tgttttagga tttatggtac tttttattcg
      241 gcaatccatt ttatttttct acttcttctt aatacctcta acacttttga tttataaact
      301 aatccaatgc atctacttgc gcttattaag ctatttcagc tagatagtac caccccatgc
      361 gtcaatgctt gattttcctt ttgtataaca ttgtataagc aataaaggac aaataagtgg
      421 tgattacacc aaaccattga ataagaaaaa ttctgatacg taaataaaca tgaacttgaa
      481 aaaattaatg aatcggttag ctactacgta gttgtctttt ttctcataca gttctgttaa
      541 cttacacttc tcttcgctag ataataaaaa atcctccaac tgtattgtcc aatataatcc
      601 caggcatcta aactagcatt atagaagaga cacacataga atctaaaata actggtttta
      661 aatgtttctt acaatcaaaa aaagctgtta a
//
LOCUS       DARBER010000280          682 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DARBER010000280 DARBER010000000
VERSION     DARBER010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.292"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            132..>682
                     /locus_tag="SCK06_002872"
     CDS             132..>682
                     /locus_tag="SCK06_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1152782.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRS"
BASE COUNT          237 a           98 c          111 g          236 t
ORIGIN      
        1 attctaagaa cgtttcaaga gatagcgaca ctatcgttat cgaaaaaggc gaaaacctat
       61 ctcattttct gctaatgtaa gttggtttag cttttgttaa tatgacttta tgaaaatgga
      121 ggtaattaaa tatgattttt cctaaagtta atgaagcaag cttgaataaa ggatcctttt
      181 ttcatatcta tgaaaacgaa gattttgaac tgtataatat cctttcacaa aaagaggtca
      241 tcgaaaacca aataatctgc catccaatta tttacaaatc tattttcaag tatgcatttt
      301 ttgcttccaa tgattttcaa agaatggaga ttaccgactg tatattcgaa aactgtgatt
      361 tttcaggctg tatattgaca aaaagcatgt tgcatcgcgt gaaatttgtc aattgtaaat
      421 taacaggtac taaattgatg gaaagttata ttgggaacac gttattcgag aattgtaaaa
      481 tggactatgt aaatttatct ggttcaaaca taaaagaaag tacctttgag cactctgtct
      541 tatcaagcgc agattttgta gactgttccc tcactaaaat aatgttttct actaatgatt
      601 tagaaaatgc tagctttttt gatactgatt taaaaaatat cgatttaagt agcaactcct
      661 ttgaaatgat tgaagtacgc tc
//
LOCUS       DARBER010000281          682 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DARBER010000281 DARBER010000000
VERSION     DARBER010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.293"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..222)
                     /locus_tag="SCK06_002873"
     CDS             complement(<1..222)
                     /locus_tag="SCK06_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEG1152783.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYK"
     gene            complement(195..>682)
                     /locus_tag="SCK06_002874"
     CDS             complement(195..>682)
                     /locus_tag="SCK06_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEG1152784.1"
                     /translation="ILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYI
                     TNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLN
                     ENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIA
                     Y"
BASE COUNT          191 a          113 c           84 g          294 t
ORIGIN      
        1 tttatagatt ttttttttgc tatccgccca gctattcaat tcgtttattt tcccaagata
       61 tttcagatct tgcattttat tttgcttatc atcaaaaaat ccatatatta acttattttc
      121 taatcttaag gtttcaataa tgacttttgc gtgtccgcca gccccaataa taatgtatgg
      181 atcactagta tattttaata agctatttgt ttttgaatcc acttaatatc ttccccatca
      241 ttcaatattt gattcaatgt ttttgttatt cttctagaac tgttgccgtc accgtagatg
      301 ttcttaacat ttttcaagct ttttataaac tgaggatctt gtattttatt caaagcagtt
      361 tcaatttcat tttcatttag atttgcattt ataatatttt tagattgttc tcgtccattt
      421 tgtctggttc ctaaatttat tactggtatt ttgaatgatg cagcttcaat tatcccagaa
      481 gaagaatttc cgatcataaa tttgcactct tttaatatag tcaggtattc ctccggctct
      541 aaattcgtaa tataaataat atccgttttt aaatatttct ggtacatttt tctgataatt
      601 tcgctaccag catcactatt aggtaaaatg caaataattg gttgagattg ttgttccatt
      661 gaattgatca aagctaaaat tt
//
LOCUS       DARBER010000282          657 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DARBER010000282 DARBER010000000
VERSION     DARBER010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.295"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            9..236
                     /locus_tag="SCK06_002875"
     CDS             9..236
                     /locus_tag="SCK06_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG1152785.1"
                     /translation="MKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDGSVR
                     MIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            233..631
                     /locus_tag="SCK06_002876"
     CDS             233..631
                     /locus_tag="SCK06_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1152786.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          236 a          109 c          113 g          199 t
ORIGIN      
        1 tgaaaaaaat gaaaaagtat aaaattagaa aatctggcaa tagcgatgta acaactatcc
       61 caccagaagt aaaagaattc atgggtgtgc agacaggtga cgcaatcagt tatgtttttc
      121 aatctgatgg ttctgtacga atgatcaaag cacaagaaga acctgatatt gattcattag
      181 ttgattcgat catgaatcaa tatgaagacg cgttaaaaga tttggttgat ttatgaggta
      241 tctttcgact aaagaaatta tcaaaatcaa cgcgaaagtt attttacgtc actcgccagg
      301 cgaaatgatc ggtgtaaaag atgcaaatgc attagatatg gccgtcaaac agccttcaca
      361 agcagtattc aatcaagaac tctatcctga aatctatgaa aaagcggcga ttttagcaat
      421 taatttagct aaaaaacatc cttttcataa tggcaataaa cgaactgctc ttgttgccat
      481 gttgttgttt cttcaattga ataattgtcc agttgctttt tctcgtcaag aagctgttga
      541 ttttatcata atgattacca ctagttcatt ggatttcgat tcactaaagt ccaagataac
      601 cagttattta cgacaaaccg caataaaatg aacgaattaa aaactttatt tctcaat
//
LOCUS       DARBER010000283          652 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DARBER010000283 DARBER010000000
VERSION     DARBER010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.296"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(180..479)
                     /locus_tag="SCK06_002877"
     CDS             complement(180..479)
                     /locus_tag="SCK06_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1152787.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          214 a          113 c           74 g          251 t
ORIGIN      
        1 taacattatt agtttatact agctgttttt gtgttaatta aaggataaaa tacaatataa
       61 agaaacatct gtttctttat ttagaataaa aaaatacaga ctctgttcaa ataaatgaac
      121 ctagcctgca ttatgagatt gactatactt tttaattcat tgagatgaat aactcgtttt
      181 tataaatgcc caaaagtaat cggtgccgaa gccattcctc gacctaattc tttttcaaat
      241 tttcctattg ttgatatctc catataaaac ggtgatactg gtttagacaa ttctcccctg
      301 attgctaacg gacgtaaact acgttccaaa ttatttatga ctttaggata atactgacct
      361 ttttctaagc ctgtctttgc gtctatcaaa agtatacgtt ccttttcttt gatctctgga
      421 tttaaaacga gatcatataa ttttagtacc atgttttcta tttcttcact tttagacatc
      481 acgatttcct cccaaaaata tatttcttcc cactacattc tataaacaaa gtatacgcta
      541 catctagatt tttttaaatc attactggat tgttaaaacc tttcaataca ctgttatggc
      601 aacgattttt attttaaaaa cattctaaat taaatcagta aaaattttta tt
//
LOCUS       DARBER010000284          640 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DARBER010000284 DARBER010000000
VERSION     DARBER010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.297"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..626)
                     /locus_tag="SCK06_002878"
     CDS             complement(<1..626)
                     /locus_tag="SCK06_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152788.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          184 a          131 c          121 g          204 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt
//
LOCUS       DARBER010000285          628 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DARBER010000285 DARBER010000000
VERSION     DARBER010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.298"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(52..450)
                     /locus_tag="SCK06_002879"
     CDS             complement(52..450)
                     /locus_tag="SCK06_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG1152789.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          204 a          133 c           81 g          210 t
ORIGIN      
        1 catacgtcct aaaacaagct gtttttgctt aatatgatca tctgagagac atcaaatcaa
       61 cttttctctc aacaaatcat tcaaagcttt cgacagatta atattctctt cagccgctaa
      121 aacatttaaa tagtgttcca cagtcactga ttttcgatca taattttgct tgtttcgagc
      181 tttagcaact ttcgtattag cttttacgac aaataaatta acattctcag atcgcttgat
      241 tcttgcattt aactcttgaa caggcgtagg cgatggaatt tctttttctt gcaactctaa
      301 aagcaccaag tatccttcca atacttcttg cgccatctca aaagcatctt ctaaagtttc
      361 accatgtgta tataagtctg gaaaatctgg gaatttaact gcatattcat ttcctttcca
      421 ttcgaatact gcacaaaaaa agtatttcat cgcattgcac ctctctaatt aaaaaataat
      481 ttttgctttt ttgtttcaaa aaaaagaatc gcctaccaaa aaaattgata ggcgatcaca
      541 agctaattaa cctctaaagg agattaatac cttgctccca aggcactata tatcttagca
      601 tacgtctgaa gcatcttcaa acgagtaa
//
LOCUS       DARBER010000286          626 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DARBER010000286 DARBER010000000
VERSION     DARBER010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.299"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            73..234
                     /locus_tag="SCK06_002880"
     CDS             73..234
                     /locus_tag="SCK06_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG1152790.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>626
                     /locus_tag="SCK06_002881"
     CDS             253..>626
                     /locus_tag="SCK06_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1152791.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNT"
BASE COUNT          258 a           62 c          108 g          198 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatac
//
LOCUS       DARBER010000287          578 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DARBER010000287 DARBER010000000
VERSION     DARBER010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.300"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>578
                     /locus_tag="SCK06_002882"
     CDS             <1..>578
                     /locus_tag="SCK06_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1152792.1"
                     /translation="IDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLDIPGNNLA
                     NIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRV
                     LSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQA
                     IGFLSNSPIGEKELLRDVSGAYLTNEYQQTNI"
BASE COUNT          234 a           65 c          110 g          169 t
ORIGIN      
        1 aattgatatt caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca
       61 agaatatgac aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg
      121 gaataattta gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa
      181 agctttggat gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt
      241 tgaaattgct gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga
      301 tagagtgttg tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc
      361 aaaaaatgga atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa
      421 gattgaaaaa atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc
      481 tataggattt ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg
      541 agcatatcta actaatgaat accagcaaac aaatataa
//
LOCUS       DARBER010000288          567 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DARBER010000288 DARBER010000000
VERSION     DARBER010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.301"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            26..>567
                     /locus_tag="SCK06_002883"
     CDS             26..>567
                     /locus_tag="SCK06_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEG1152793.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYR"
BASE COUNT          204 a           92 c          114 g          157 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcg
//
LOCUS       DARBER010000289          558 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DARBER010000289 DARBER010000000
VERSION     DARBER010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.302"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>558
                     /locus_tag="SCK06_002884"
     CDS             <1..>558
                     /locus_tag="SCK06_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1152794.1"
                     /translation="QINDLYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTP
                     LASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYL
                     KINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVN
                     GVINIGIVNDWLYIENSYGNNKISN"
BASE COUNT          249 a           45 c           77 g          187 t
ORIGIN      
        1 acaaattaat gatttgtatt ctactttatt aagaacaatt gacgatttgg aatttaaaaa
       61 taaagaaatt ttaaaattag aaaagttaaa atatgacttt tttaaaggtg catcccatga
      121 acttaaaacc cctcttgcta gtcttaaaat aatactggaa aacatgaaat acaatattgg
      181 aaaatataaa gaaagagata tttatattaa tgaatgtatt gaaatcgttg atagtttaac
      241 aaaaaatatt tctcaaatat tatcagttca ttctatagaa aatttgaata atgatgaaga
      301 atatttgaaa ataaatgata cattagaaga catattaaaa aagtatgaaa tattagcaaa
      361 tcaaaagaaa ataactgtca ataattatat attagacgag aatgtttata taggaaaaac
      421 agctttaaag attattctat ctaatttgat tagtaatgcg gtaaaatata ctgatgtaaa
      481 tggggtaatt aatattggga tagttaatga ttggttatat atagagaatt catacggtaa
      541 caataaaatt tcgaatat
//
LOCUS       DARBER010000290          557 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DARBER010000290 DARBER010000000
VERSION     DARBER010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.303"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            171..527
                     /locus_tag="SCK06_002885"
     CDS             171..527
                     /locus_tag="SCK06_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1152795.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          217 a           80 c          106 g          154 t
ORIGIN      
        1 ttttataaat aaaaaaatgt gttttttgag aaaaacttca agatagtcct taactttctt
       61 ttgaagcaat aagaacaaaa tgaggaaaag tttaaaattc agctgttgcc aacaactttc
      121 aatagacgaa atcttctatt tcgttaataa taaggaaaga ggtgaagtac atgaaaaaat
      181 taattggcgt acttgtcgtt ttaggcatat ttctcggcgg aagttttgca gcttatcatt
      241 atttttacgg cggcgaagct tattatacaa aaatcacgac gaatggcgaa atgtcagcta
      301 cccaagcaga taacggagag aaattcacga cttataccta caagcaagcg gcttataata
      361 aagatggtga aaaaaaacag gtgacgcttc gcgaagaaag agaacatcca ttaaagatga
      421 atgcgtatct taaactcaaa gtcaatcctc gtaaaggtgt attaagctgg gaagaaataa
      481 aggcttctga agtgcctaaa aatgcggcag aaaaaatcga taattagtaa atggtcttaa
      541 aattttatta gataaaa
//
LOCUS       DARBER010000291          545 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DARBER010000291 DARBER010000000
VERSION     DARBER010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.304"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            261..>545
                     /locus_tag="SCK06_002886"
     CDS             261..>545
                     /locus_tag="SCK06_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEG1152796.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWN"
BASE COUNT          226 a           80 c           86 g          153 t
ORIGIN      
        1 atgaatctca gtttaatctc gaaaaattta tggagtatta ttctattcag aataagctca
       61 gatacacttg ttatgctgtc tgccaatgct attttaagaa aaaatattct tacgcaaggc
      121 aatatgtatg aatacatcaa gaattattaa aaaaaccgat ttcagcaaaa cattaaaaaa
      181 gcccgtgctg caacacgggc tctttcctca tatatgagct tctacaaaaa aaatcatatc
      241 acagaaatga ggaagaaaaa atggaaaaag agattttaat aaaatctcca ggaaaaacct
      301 tgattaaagt aactgatgat tctatttcaa ttattagaaa aggatttata aatttagtta
      361 atcaaggtat taaaggagaa aaaactatcc cctttaaaaa tatatcagcc gtacaattaa
      421 aaaaaccagg tatgagtaat ggttatatac aatttacctt gctaggcgga aacgaaagtc
      481 gcggaggtat attagcggcc acgaaagatg aaaacaccgt tatgtttaca aaaaaatatt
      541 ggaat
//
LOCUS       DARBER010000292          528 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DARBER010000292 DARBER010000000
VERSION     DARBER010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.305"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..>528)
                     /locus_tag="SCK06_002887"
     CDS             complement(37..>528)
                     /locus_tag="SCK06_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152797.1"
                     /translation="DVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLL
                     LINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIIT
                     NRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIID
                     RIE"
BASE COUNT          168 a           94 c           84 g          182 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatc
//
LOCUS       DARBER010000293          526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DARBER010000293 DARBER010000000
VERSION     DARBER010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.306"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCK06_002888"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCK06_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG1152798.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="SCK06_002889"
     CDS             complement(349..>526)
                     /locus_tag="SCK06_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152799.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARBER010000294          525 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DARBER010000294 DARBER010000000
VERSION     DARBER010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.307"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(242..391)
                     /locus_tag="SCK06_002890"
     CDS             complement(242..391)
                     /locus_tag="SCK06_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152800.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          165 a           82 c           82 g          196 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttc
//
LOCUS       DARBER010000295          514 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DARBER010000295 DARBER010000000
VERSION     DARBER010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.309"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..293
                     /locus_tag="SCK06_002891"
     CDS             99..293
                     /locus_tag="SCK06_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152801.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          216 a           66 c           86 g          146 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttat
//
LOCUS       DARBER010000296          501 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DARBER010000296 DARBER010000000
VERSION     DARBER010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.310"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            143..400
                     /locus_tag="SCK06_002892"
     CDS             143..400
                     /locus_tag="SCK06_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152802.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          211 a           65 c           70 g          155 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaatag
      481 attttaaatt tattgtccat a
//
LOCUS       DARBER010000297          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DARBER010000297 DARBER010000000
VERSION     DARBER010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.312"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SCK06_002893"
     CDS             complement(62..259)
                     /locus_tag="SCK06_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1152803.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARBER010000298          444 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DARBER010000298 DARBER010000000
VERSION     DARBER010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.313"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          151 a           72 c           95 g          126 t
ORIGIN      
        1 gcaataattg caacaaagaa atttcatcgg gggaagaggt ttggacaaaa atggaaattt
       61 ccgccaaaag taactgctcc tcaactgaaa tcaattaaag aactagaatt tgagaatgca
      121 ctggtaattt gtatgacatg tactgatgaa gtatttcgaa aacattttta aaggttgaga
      181 tgtatataga tttaaccaat catttatctc tctcggaaaa ggaatattaa ctaaccagcg
      241 ggttatcaaa aataggtagg aaaactgtac ttaatggctt atttcgcgct gattatgaga
      301 atatcagtct gtttgattaa tttttagggt tcaaaataca gagacaaact ccaggtcggg
      361 tttcgccaca agcgaattac ccgatctgga gttttgtcta ttgcaggaag gcgtagccgg
      421 actgcaggaa taaagccccc ggtt
//
LOCUS       DARBER010000299          415 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DARBER010000299 DARBER010000000
VERSION     DARBER010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.314"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..339)
                     /locus_tag="SCK06_002894"
     CDS             complement(<1..339)
                     /locus_tag="SCK06_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152804.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DARBER010000300          414 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DARBER010000300 DARBER010000000
VERSION     DARBER010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.315"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            124..327
                     /locus_tag="SCK06_002895"
     CDS             124..327
                     /locus_tag="SCK06_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEG1152805.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          170 a           44 c           79 g          121 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcat
//
LOCUS       DARBER010000301          399 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DARBER010000301 DARBER010000000
VERSION     DARBER010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.316"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DARBER010000302          365 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DARBER010000302 DARBER010000000
VERSION     DARBER010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.317"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          136 a           53 c           46 g          130 t
ORIGIN      
        1 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
       61 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
      121 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
      181 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
      241 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
      301 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
      361 gataa
//
LOCUS       DARBER010000303          352 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DARBER010000303 DARBER010000000
VERSION     DARBER010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.318"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>352
                     /locus_tag="SCK06_002896"
     rRNA            <1..>352
                     /locus_tag="SCK06_002896"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          110 a           74 c          105 g           63 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gc
//
LOCUS       DARBER010000304          348 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DARBER010000304 DARBER010000000
VERSION     DARBER010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.320"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>348
                     /locus_tag="SCK06_002897"
     CDS             <1..>348
                     /locus_tag="SCK06_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEG1152806.1"
                     /translation="NYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITL
                     TTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFL
                     FSEVGYFTSKGDLL"
BASE COUNT          123 a           44 c           58 g          123 t
ORIGIN      
        1 aattacccgt ctatttttaa gcactatcta ttttttatta acgaagtaga agatcggata
       61 aaaaataatt cattatctag tagtgtatct agttttaaga agatgtttaa atctcgagat
      121 ggtatcacac ttactacagc acataaagtt aaaggaaatg agtatgatac tgttatcgct
      181 attaatcttc taaacgggaa aattcctcat tggaatgaaa tttttaatgt ttctgataaa
      241 ggaattagtt cagcgagaaa actactatac gtaatttgtt caagagcaaa aaataattta
      301 tttcttttct cagaggtggg ttattttacg agtaaaggag acttgctt
//
LOCUS       DARBER010000305          345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DARBER010000305 DARBER010000000
VERSION     DARBER010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.321"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>345)
                     /locus_tag="SCK06_002898"
     CDS             complement(<1..>345)
                     /locus_tag="SCK06_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152807.1"
                     /translation="AYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGV
                     LGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETS
                     QLIRRELTKIHFK"
BASE COUNT          109 a           60 c           48 g          128 t
ORIGIN      
        1 tttaaaatgt attttggtta actctcttcg gatcaattgg ctcgtctctt ctcgcaacag
       61 attaaactga ttttcaaaac tttcaatgaa tcgttcattc aaccattcga ttgtttcatc
      121 ttcatttttt ctcaagtatt tttgatccaa tttatcatga agataaagcc aagagagtgt
      181 cttattgata aaaataacgg atttttctcc taatactcct tttaagtggt aaaatcctat
      241 atccgaaaaa aatagcgact gattgctagc aatatctaaa gaatcagctt gttcagccaa
      301 caggttgatt gctaaaaagt ggaataattt ctttttttca taagc
//
LOCUS       DARBER010000306          341 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DARBER010000306 DARBER010000000
VERSION     DARBER010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.322"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>341
                     /locus_tag="SCK06_002899"
     CDS             <1..>341
                     /locus_tag="SCK06_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152808.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DARBER010000307          338 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DARBER010000307 DARBER010000000
VERSION     DARBER010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.323"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..338
                     /locus_tag="SCK06_002900"
                     /pseudo
     CDS             1..338
                     /locus_tag="SCK06_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DARBER010000308          331 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DARBER010000308 DARBER010000000
VERSION     DARBER010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.324"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>331
                     /locus_tag="SCK06_002901"
     CDS             <1..>331
                     /locus_tag="SCK06_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HEG1152809.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DARBER010000309          328 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DARBER010000309 DARBER010000000
VERSION     DARBER010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.325"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            52..>328
                     /locus_tag="SCK06_002902"
     CDS             52..>328
                     /locus_tag="SCK06_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1152810.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          104 a           60 c           63 g          101 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DARBER010000310          308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DARBER010000310 DARBER010000000
VERSION     DARBER010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.326"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(76..>308)
                     /locus_tag="SCK06_002903"
     CDS             complement(76..>308)
                     /locus_tag="SCK06_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1152811.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DARBER010000311          305 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DARBER010000311 DARBER010000000
VERSION     DARBER010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.327"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCK06_002904"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCK06_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEG1152812.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DARBER010000312          282 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DARBER010000312 DARBER010000000
VERSION     DARBER010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.329"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>282
                     /locus_tag="SCK06_002905"
     CDS             <1..>282
                     /locus_tag="SCK06_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG1152813.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DARBER010000313          281 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DARBER010000313 DARBER010000000
VERSION     DARBER010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.330"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..281
                     /locus_tag="SCK06_002906"
                     /pseudo
     CDS             1..281
                     /locus_tag="SCK06_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          109 a           56 c           55 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca g
//
LOCUS       DARBER010000314          270 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DARBER010000314 DARBER010000000
VERSION     DARBER010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.denovo.333"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>270
                     /locus_tag="SCK06_002907"
     CDS             <1..>270
                     /locus_tag="SCK06_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1152814.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DARBER010000315        14792 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.054, whole genome shotgun
            sequence.
ACCESSION   DARBER010000315 DARBER010000000
VERSION     DARBER010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.054"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            146..1042
                     /locus_tag="SCK06_002908"
     CDS             146..1042
                     /locus_tag="SCK06_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1152815.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1192..2133)
                     /locus_tag="SCK06_002909"
     CDS             complement(1192..2133)
                     /locus_tag="SCK06_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1152816.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2259..3380
                     /locus_tag="SCK06_002910"
     CDS             2259..3380
                     /locus_tag="SCK06_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1152817.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3638..5140
                     /gene="eat(A)"
                     /locus_tag="SCK06_002911"
     CDS             3638..5140
                     /gene="eat(A)"
                     /locus_tag="SCK06_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1152818.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5168..5734
                     /locus_tag="SCK06_002912"
     CDS             5168..5734
                     /locus_tag="SCK06_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1152819.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5945..7006
                     /locus_tag="SCK06_002913"
     CDS             5945..7006
                     /locus_tag="SCK06_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1152820.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7009..7905
                     /gene="murQ"
                     /locus_tag="SCK06_002914"
     CDS             7009..7905
                     /gene="murQ"
                     /locus_tag="SCK06_002914"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1152821.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7919..9901
                     /locus_tag="SCK06_002915"
     CDS             7919..9901
                     /locus_tag="SCK06_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1152822.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9966..10412)
                     /gene="nrdI"
                     /locus_tag="SCK06_002916"
     CDS             complement(9966..10412)
                     /gene="nrdI"
                     /locus_tag="SCK06_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1152823.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10623..12047
                     /locus_tag="SCK06_002917"
     CDS             10623..12047
                     /locus_tag="SCK06_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1152824.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12228..13001)
                     /locus_tag="SCK06_002918"
     CDS             complement(12228..13001)
                     /locus_tag="SCK06_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1152825.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12998..13903)
                     /locus_tag="SCK06_002919"
     CDS             complement(12998..13903)
                     /locus_tag="SCK06_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1152826.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14057..14377)
                     /locus_tag="SCK06_002920"
     CDS             complement(14057..14377)
                     /locus_tag="SCK06_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG1152827.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4748 a         2623 c         3114 g         4307 t
ORIGIN      
        1 aattttaata gaagaatgat ttatttcatc atattttcat acaaatgtta aattttcata
       61 caaatatagg aaacgtttta ctggcttttt actatttaaa caggctatca taggaaaaag
      121 tgacagagaa agtgaggcgg aaaagatgaa caaaaatgac gtactgctag ttgcaaaaaa
      181 taaatcactt tattttttgc cttccatccg ctctttagat gtttggtccg gaaaagattt
      241 actacagtcg gaattcatta aatcagctag acaacagttg gcaaattatc agctgatcgt
      301 ttttttagat tatggatttg atatggagat ggctcgacgc gtgcgcccat ttaccaatgc
      361 aaaaattgtg ttattttttt ggaatcactt caaagcagaa cacgaggcga tgctgaagca
      421 ggcacaagat gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga
      481 acttgggctg aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga
      541 accaaagacg gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag
      601 ctataagcag gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg
      661 agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc
      721 gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga
      781 atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt
      841 ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt
      901 tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa
      961 ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct
     1021 ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa
     1081 caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct
     1141 taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc
     1201 gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc
     1261 aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa
     1321 acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg
     1381 atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt
     1441 tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg
     1501 cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt
     1561 tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt
     1621 tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt
     1681 ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc
     1741 tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc
     1801 ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg
     1861 gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac
     1921 gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc
     1981 tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca
     2041 acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc
     2101 cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt
     2161 tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa
     2221 aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg
     2281 attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg
     2341 aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag
     2401 aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat
     2461 tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca
     2521 tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat caaactatcc
     2581 gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag
     2641 aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc
     2701 aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa
     2761 aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag
     2821 atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag
     2881 gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa
     2941 taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta
     3001 ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc
     3061 aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc
     3121 ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa
     3181 aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt
     3241 ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa
     3301 tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt
     3361 tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat
     3421 ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt
     3481 cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt
     3541 tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg
     3601 aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa
     3661 tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt
     3721 tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact
     3781 gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc
     3841 ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat
     3901 tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga
     3961 aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc
     4021 acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga
     4081 tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt
     4141 ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa
     4201 aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg
     4261 tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa
     4321 aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca
     4381 agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc
     4441 tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga
     4501 gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc
     4561 gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt
     4621 attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa
     4681 cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat
     4741 aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga
     4801 caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt
     4861 gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat
     4921 gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact
     4981 atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca
     5041 actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga
     5101 taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca
     5161 acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac
     5221 gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc
     5281 gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc
     5341 tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc
     5401 agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt
     5461 tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt
     5521 cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt
     5581 attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt
     5641 gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat
     5701 ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa
     5761 atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac aattcttttg
     5821 ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag tattgaaatt
     5881 ttgtttcttt agatatatac tctagtcata gaaatgaaga cggatacaga aaaagaggga
     5941 taatatgttt ggcttttcaa tatttatgaa cgattctttg tcagaagaga agaaacagta
     6001 tatccgccaa atggcatcca atggctttgt cggtattttc acttccatgc atataccaga
     6061 agatgatgtt tcggcatatc aaaagcgatt gatcgaacta ggaagcgaag cgaaaaagaa
     6121 taaactagaa ttgatggtcg atatttcagg agaggcattg gaacgagcgg gtttttcatt
     6181 agaagatcta acgcctttga aagcaattgg tgtaacaggt ttaagaatgg attatcatat
     6241 ttccaatcag cagattgccg actggtcaca tcaattgaaa atcagtttga atgctagcac
     6301 gatcacgcct aaagatatcg atgaattgaa agaagcagaa gctgatttct cacagattga
     6361 agcttggcat aattattatc cacgtcccga aacagggctg gacaaagaat ggtatcagaa
     6421 aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa ggttttgtac caggagatac
     6481 agagttgaga ggcccactat atcaaggatt gccaactttg gaagaacatc gagggatcca
     6541 tcctcttgca ggcgctctag atctattagc aagtaacacg gatattgttt acatcggaga
     6601 tgcaggtctg tcagaaaacg ttcaggagca atttgcttct ttccaaaaag aacagacagt
     6661 cttgctccat acggaacctg tagatgaaga attttacgaa tatatattag ggaagcatac
     6721 gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta gatgcacgtt ttcgagagat
     6781 tccgcctatc ccagcaagaa atacagctac gcgtatgaaa gggtctatta ctttagataa
     6841 cgaaaagtat ttgcgctata tgggtgagat ccaattaacc aaatatgatt tgccagcgga
     6901 tgaaaaagtg aatgtagtag ctaaagttat caaagaagag ctaccattga tcaaccaaat
     6961 caaagctggt atgaactatc aatttataag aaaagaagga agataaaaat gagcaaaatc
     7021 aatttagaga acctaacaac ggaacgcaga aaccaagaaa cttttggatt agatgagatg
     7081 agtatttctc aagccgttca actaatgaac aaagaagatc agaaagtggc cgctgcggtt
     7141 gaaaaagaac tgacaagtat cgaaccagtg attgctaaca cgattgagtc ctttaaaaaa
     7201 ggtggacgtc tgatttatat gggagccgga acaagtggaa gactaggtgt gttagatgct
     7261 gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg tcataggtct aattgctggt
     7321 ggagaagaag caatgactgt agctgtcgaa ggggccgaag attcttatga gctagggcaa
     7381 gaagatctaa tcaagctgaa tttgacaaaa aatgatatag ttattggtat cgcagctagc
     7441 ggtcgtacac catatgtgat cggtggatta gattacgcaa aatcgatcgg tgctgtcaca
     7501 ggaacgattt cttgcaataa acaagccgaa atcagtaaac acgcggattt cccaatcgaa
     7561 gtcgatgcag gtccagaatt tttgacaggc tctactcgat tgaagtcagg aacagcacaa
     7621 aaacttatct tgaatatgat ttctactatc agtatgattg gtataggtaa agtattcaat
     7681 aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa tcgaacgctc aaaacgaatc
     7741 atcatgcaag cgacagaagc tgattacgaa acagctgaaa agtactttga agaagcagat
     7801 caaaatgtta agctggctat cgtcatgatt ttgacaaata gtgataaaga ggaagctcaa
     7861 gcaaaattag ctgaagcaaa cggatttgta aaagggacct tatgaggggg aaaagaccat
     7921 ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa atttataaag gggcaggcgg
     7981 catgggcaat gtagaaagca ttgtccactg catgactcgt gtgcgtatga agatcatcaa
     8041 tgatgatctt gtaaatgtct ctgaattaaa agcgattcca ggagtgcttg gtgtagtaga
     8101 agatgaacag cttcaagtca tcatcggacc tgggaaagtg aacaaagtag cacagcaaat
     8161 ggttgatttg gcaggcgtga aattaggaga aaaactttct tctgtaagta cagctacagc
     8221 aaattcatca gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc
     8281 acaaaaatcg aaacaaaaac catctaaaat caaatcggta ttaaaagaca tttctaatat
     8341 ctttgtgcca atgattcctg catttgtcgg tactgggatc gtggcaggga ttgccgcagt
     8401 tttagcaaac ttagtcacag ccggaacact tgatgcagca acatggcaac aatatatcga
     8461 tgtgatgaac atcttgaaaa acgggatgtt tagctaccta gttatctata caggtatcaa
     8521 tgctgcacaa gtttttggtg caacaccaac tcttggtgga gttatcgggg ccgttgtgat
     8581 gctgacaggg atgaatccgg aagcaccact gaaaaactta tttactggtg aagctcttgc
     8641 agctggacaa ggtgggatcc taggtgtcat cttcgctgtt tggctattgt caatcgttga
     8701 gaaaaaactg cataaagtag tacctgatgc ggtagatatc atcgttacac caactctttc
     8761 attgattgcc atcggtctga ttgaaatttt cttcatcatg cctttagcag gtttcatttc
     8821 tgatggcatg gtcggtggga tcaactgggt acttggcgtt ggtggagcat ttgccggctt
     8881 cgttctaggc acattattct tgccgatggt tatgttcgga ctgcatcaaa ttttgactcc
     8941 aatccatgtg caaatgattg atgaaactgg tcgtacgcta ttgcttccaa ttcttgcgat
     9001 ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg attcgctgca aaaaagataa
     9061 agaattaaca gaaatgatca aaggagcttt accagtcggg atcctaggaa tcggcgaacc
     9121 attgatttac ggagtaacat tacctctagg acgcccattt attactgctt gtatcggtgg
     9181 aggtatcggc ggggcagtga tcggaatgat cggaaatgtc ggggctatcg ctattggacc
     9241 ttcaggagcc gctttgatcc cattgatctc tgacggtaaa tggttaggct acatccttgg
     9301 attattggca gcttatgctg gtggcttcgt tgctacattc ttcttcggta ttcctaaaga
     9361 ccgtttagaa aaagaagaat tagcgactga aacagccaca gtaaatgaag tgcctgaaaa
     9421 agtagtacct caaacttcag ctgaaagtgt gactatcctc tcatcagttg ctgacggaac
     9481 agttgtttct ttagataaag caagtgatcc agtttttgca caaaagatga tgggtgaagg
     9541 ctactttgta gaaccagaaa acggacatat ctattctcca gttactgggg tagtcagctc
     9601 tgtcttccca acgaaacacg ctattgggat cacaacgcca aatggtttag aaatcttgct
     9661 tcatatggga atcaacacag ttgacttagg cggtacgcca tttgatgtga aagtaacaga
     9721 agggcagcaa gtgacacctg atacgcttgt agcagaagct gacttagaag cgatcaaagc
     9781 agcaggcaaa gaaacatcca tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt
     9841 tgtcttggaa aaaacaggta aagcaaaagc aaaagcacct gtgatggatg tcaaagctta
     9901 atatgataga aacaatagaa acgaaagaag aaactgaaat ctatttttca gcttcttctt
     9961 ttttattatt tattgttttc tgctaaaatc tgataaattc gtgtcacatc agatggtgtt
    10021 ccccgtaatt cataatctgc caagaaagga aagccaaatg tttgtgcata ttgtttggct
    10081 gttagacaat actgattatt gaagttttta tttccgctac cgactacacc aagacaaagt
    10141 ttatgattgt cttcatgttc cagatattca cgcatcgttt cggtcaagat ttctgtatca
    10201 ccgttatcta aaccatttcc gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct
    10261 gtttctttcg taaaatcact attttcatgg atttctttta aagtaacttc aggattgaca
    10321 ggatcttctg catgcatttt ttcagcatat tctgctagat gtttggcaaa ggcacgcgta
    10381 tttcctgaaa tcgatatata taaaatgttc atggatcatc ctccaattct ttccttttag
    10441 tatagcatct tttttaagtt ttttggtggt gtaatttgga aagggactag gcgctttgct
    10501 tgtataatga gaaaaaagcc ggtagaatta tatttctatt aaagttttta ttaattaagt
    10561 aaaaataaaa aaggtttgtt atactttttg ataaaagcgg ttttattttg gaggatgctt
    10621 acatggatac agagcaacag aaaaaaggaa ttggtttaat tccacttgct gccttagtaa
    10681 ttggttcagc gattggaggt ggcgtatttg gtattatgac ggacatctcc ggatcggctg
    10741 gcggcccagc tattttcagt tgggtactcg taggaacagc aatgttgatg ctctccttgt
    10801 cgatcaataa tatcaataaa aaacgtccag atttaaaagg cgggatcttt agttacgcag
    10861 aagaaggttt tggccgtttc gctggtttta tcagcggttg gggttactgg ctcacatgct
    10921 ggttaggcaa cgtagctttt gcaacattgt tgatgagcgc attaggatat ttcttcccag
    10981 tatttgaagg tgggcaaaat ctggcatcga tcgtgatgag tacagtcttt ttatggggat
    11041 atgcatggct agtgaatcgg ggagtcgaaa atgctagttt cgtcaatgct atcgtcacat
    11101 tctgtaaatt agtgccatta tttttattta ttgtagtagc tatcagcacg ttttcgatcc
    11161 aacaattcat tgataacttt tctagcgaca ttatattgaa tgcgaatgga cagccgatcc
    11221 cgttttccag tcaagtattg agttgcatca tggtgatgct ttgggtattt gttggtattg
    11281 aaggagcctc tgtcgtaggt tcacgagcaa aaaaacaatc agatgttggg aaagcaacga
    11341 ttctagggat attcggcttg attattattt acgtcttggt ttccatgctt ccatatggga
    11401 caatgacgat gaatcagctg caaacgatca aggcgcagcc tgctatgggc tatgtgtttg
    11461 aaatgatggt tggtaaatgg ggagcagtcg tgatcaacgg cggtttgatc atttctttag
    11521 ttggtgtttg gctctcatgg acgatcttac cgattgaaac aatgggaaac atggctgaag
    11581 acggactttt gcctaaagca tggggaaaac tcaacaaaaa tggggcacca acttatgcta
    11641 tcgtattgac gacgttatgt acgaatgtct ttttactttc attattattc actgacagtg
    11701 cttataactt tgcttacaca cttggtacag cagctatttt cttcacgtgg cttttcttag
    11761 gattgtacca aatgaaactt tcttatcagc ggaaagaatg ggtccaattt ttagtagggc
    11821 ttttagcaag tgcctttcaa gtatgggcta tgctagtcgt agcctttaat gaagtaatgc
    11881 ttgttcttgt tcttttcttg ccaggcattt atttttacct caaagcacgt aaggagcaaa
    11941 acgagcctgt acaacatatc cacttagata agattttact tataggtgtg acgatggtcg
    12001 gagtgattgc aatggtttta tttgccactg ggatgcttca cgtatagaag tataaaagaa
    12061 gtataaagag aggttgtgag cctgtcgttt attcaaaaac atactaggaa aggagctgtg
    12121 acacaaatgt tgtcacagct cctttttcag aataaacggt gtttaagaga cagccaatcg
    12181 gcaataaggc tgaacgtctc tttcacagcc tcttttttga tgagtcatca tacatttttt
    12241 cgcttaaata cgagatatcc taatgctaag aacaaaacga tgtaagctaa attaccgaat
    12301 agcatttgat tagtagaaag atgagtcaat tgcttcatga tctcttcgtt tccaatttgg
    12361 ttttgcagat tcaacatact gatcggattc catttgatcc actcccattt ttggatgagt
    12421 gcaataagga taccagatat gattgacgaa gcaaaataaa agacgattcc tgctgagatg
    12481 gatgcgccgg aactattgat aaagcaagca atcatcaaca ctaaacttaa aatcagccat
    12541 aaaccgatat aactacccaa agtataagtg aacaagcttt ggatcagtgt ttgaccagta
    12601 gaaaccttct ccgtgaagga aatcgaaggg aaaagaatca gtttcatcaa cacagtgaca
    12661 ataatagcta aaaggtataa ataaacagaa taaataacta atgtgatcca tttgcttact
    12721 aatatttctc ctcgagaata ctttcgataa aggagatttt tcaatgtacc atattgtgct
    12781 tccatcgaaa tgatcgtgct tgcagctgca atcatgataa agacaatcca agatgtggca
    12841 ctaaaaagct gagcagtcat ttcaactgga tcaatgatcg tcgtgtattt tttggctaga
    12901 agtgctgttc ctatgagcaa aacaactaat agaacagata aaacagcagt tgactttttc
    12961 ttgatcaatt taaataattc ttgttttaca agcaatgtca tttttcttct tcccccttta
    13021 ctgaatccag aatttccaat agagaagatt ccaaatcatt cgtcagatgc tggacgtctg
    13081 tcacatggat attttgtgca gacaatagct ggatcacggt tcctaataat tcttcgtttt
    13141 cttttaaaag cttgatttta tcaggatctt ccaaagtata tccagcttct gtcaagatcg
    13201 ttcgagcagc tagatcattt gttgtagaga taacgatgaa atcttgcgct gtttggttca
    13261 attcagccat tgtacattgt ttgatgatct ttccttgatt gatcaggatc agctcatcga
    13321 ccattttttc aagctcgctg agtaagtgac tggagatcaa gaaagaggtg ccctgagtag
    13381 ccaagtcggt aatgattttt cgcacatctc ttgttgcttg tggatccaaa ccgttcatcg
    13441 gctcatctaa aataacgata gacggatggt tcagtaaggc aagagcaatt cccaattttt
    13501 gtttcattcc caacgagtag gatttcgctt ttttgtgaat gtacttttcc atcttgagct
    13561 gattcacgat agtgtcgata gcagatacat cgttggtttc agagaataac ttcagatgat
    13621 catagcctgt taagaatgga tagatacccg ggtattcgat caatgctcct acttgttcca
    13681 accctttatg actagaggga gaaatctcat tttcaccaat acagattgtt ccatctgagt
    13741 aatggatcag tcctaaaata gctttcatga tcgttgtttt tccagcgcca ttaggaccta
    13801 ctaatccaac gattttgcca ggggataagc taaaactgac atcgctgagg gcttgtttgt
    13861 gaccaaagtt cttggataat tcttggaccg aaagactttt caaaaatatt cactcctttt
    13921 ctatagatag tacaagcata cacctgatga gtggagaagt cgctaggtca ttggacttat
    13981 ttcttatttt ttcataaaaa aattgaatat taaataaaaa ggcttatttt ttctgatcag
    14041 tacttattcc aagtgtctag tggccgctag taatcttgat gccaatgata ctgatgagta
    14101 gcatccccac aaagaaccaa gtggaaagat tcagcttatc atgaaacaag accacaccaa
    14161 ttatgaccga accaactgcg ccaatccctg tccagatagg ataagagaga ctcatcggca
    14221 aacttttgat tgcctttgac aaaaagtaaa aactagcaat catgccaatc actgtgtaaa
    14281 aagaataatt gattttagaa aaaccatcac tccatttcat catcgttgac cagaatactt
    14341 ccaaaattcc tgctatgatt aattccatcc atgccatact tacattcctc cttaactata
    14401 aaaaaataca caaaaagagg aaaatcatcc cttcaaagac ctaatggaat gatcatcctc
    14461 tttatcctac ccggtggcag attgtatatc tttttctgta gtatagcaca cctatccaga
    14521 tatcggcaat ctcatacttt ttagtaaagt gtaccaagca ctgacggata tatcgttagc
    14581 ggaaaagaag atgaatgctt tgcaatttca tagaaagaag ttgttattta ttaatcattt
    14641 aaaaaaaaca gtaactagac cttatttcta tatattatcg ggcaataaat taaaaaaagc
    14701 tttaccttac aataagtaag ataatttttg tgataaaaaa ataacgaaaa atatttaaaa
    14761 atagtgtcta tttcgtgaga aaacaactat at
//
LOCUS       DARBER010000316        11249 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.086, whole genome shotgun
            sequence.
ACCESSION   DARBER010000316 DARBER010000000
VERSION     DARBER010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.086"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            255..1295
                     /locus_tag="SCK06_002921"
     CDS             255..1295
                     /locus_tag="SCK06_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1152828.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1393..1581
                     /locus_tag="SCK06_002922"
     CDS             1393..1581
                     /locus_tag="SCK06_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1152829.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1639..1992)
                     /locus_tag="SCK06_002923"
     CDS             complement(1639..1992)
                     /locus_tag="SCK06_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152830.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2523..2945
                     /locus_tag="SCK06_002924"
     CDS             2523..2945
                     /locus_tag="SCK06_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1152831.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2942..3172
                     /locus_tag="SCK06_002925"
     CDS             2942..3172
                     /locus_tag="SCK06_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152832.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3670..3870
                     /locus_tag="SCK06_002926"
     CDS             3670..3870
                     /locus_tag="SCK06_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1152833.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3897..5090
                     /locus_tag="SCK06_002927"
     CDS             3897..5090
                     /locus_tag="SCK06_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1152834.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5158..6723)
                     /gene="guaA"
                     /locus_tag="SCK06_002928"
     CDS             complement(5158..6723)
                     /gene="guaA"
                     /locus_tag="SCK06_002928"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1152835.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6782..7705)
                     /locus_tag="SCK06_002929"
     CDS             complement(6782..7705)
                     /locus_tag="SCK06_002929"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1152836.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7901..8506
                     /locus_tag="SCK06_002930"
     CDS             7901..8506
                     /locus_tag="SCK06_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1152837.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8814..9830
                     /locus_tag="SCK06_002931"
     CDS             8814..9830
                     /locus_tag="SCK06_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1152838.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9971..>11249
                     /locus_tag="SCK06_002932"
     CDS             9971..>11249
                     /locus_tag="SCK06_002932"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1152839.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTL"
BASE COUNT         3684 a         1999 c         2236 g         3330 t
ORIGIN      
        1 gttatattat cataatacca caaaatcaca aattttcatt caattatcat tttcaattgc
       61 attttataaa tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca
      121 ttttgaagta cattttgtca ttttgtactc caaaactgaa ctacttactt gcatatatac
      181 gcctttatag tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa
      241 taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt
      301 attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag
      361 atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa
      421 ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct
      481 cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt
      541 ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg
      601 tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag
      661 aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg
      721 tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact
      781 taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat
      841 tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtacggttta tactatagcc
      901 ctgttagtac tccagcagat tttcggatgc ttactcctat tgtactggag caagccttta
      961 gaaaagctgg aacagaattg ttagagccat atcttagttt taaagtttat gcaccacagg
     1021 aatatctttc acgggcatat aacgatgctc ccaaatattg tgcaaatatc gtaaatactc
     1081 aactgaaaaa taatgaggtc attattattg gagaaattcc tgctcgatgt attcaagatt
     1141 atcgcaatga tttaactttt tttacaaatg ggcttagtgt ttgtttagca gagctaaaag
     1201 gatatcaggt taccactggc gaacctgttt gccagacccg tcgtctaaat agtcggatag
     1261 ataaagtaag atatatgttc aataaaataa cttagtgcgt tttatgttgt tatataaata
     1321 tggtttctta ttaaataaga tgaaatattc tttaatatag atttgaatta aagtggaaag
     1381 gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca
     1441 agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt
     1501 aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa agagccagac
     1561 gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg ctctttttgt
     1621 ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac cttttgctag
     1681 tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt ctacctgtaa
     1741 ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa tagatacatg
     1801 gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt tgttttctat
     1861 atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac gagtgagacc
     1921 ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt atttatcttc
     1981 ttttttacgc atactaaatc acctctatac attttactgt tcctatataa ccataaacag
     2041 gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg aatttattaa
     2101 tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat acaactttat
     2161 aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga gggaaaagtc
     2221 gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc aaaaaaactt
     2281 atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac atagatagaa
     2341 aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg tcacttaata
     2401 aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg tcggggttta
     2461 gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag ggggtgaaaa
     2521 gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac
     2581 aagttattga agatgaacga aaagattact tcaaacatct agacagacta tcagcaaaag
     2641 aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact gttgatacgt
     2701 acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt gaaagtgatt
     2761 tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt ttacttcatt
     2821 actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat cgttcgactg
     2881 tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag
     2941 aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga
     3001 cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt
     3061 acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa ccctacgtgg
     3121 cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat agtcccattt
     3181 tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt tgttattcca
     3241 tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac gttctttgac
     3301 aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc
     3361 atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc tttagcagga
     3421 ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata gtccgagcgt
     3481 tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt gaaattcctg
     3541 tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt gtattattat
     3601 aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat aattgggaaa
     3661 ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata tggaacttct
     3721 ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg gcatatctac
     3781 tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat tctaggaatt
     3841 cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta aaaactatgg
     3901 ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca aatctaggtt
     3961 atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact aaaaaagaag
     4021 caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt gagaataagg
     4081 ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat aagacacagg
     4141 taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc tcttattttg
     4201 ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa ctaaaattat
     4261 caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca gttgctatgg
     4321 atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt ggcaatgtga
     4381 aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa gtaatctctc
     4441 taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg tttttattta
     4501 tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt gattttgact
     4561 caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat tatagatttg
     4621 ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc acattaactt
     4681 atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt gtaatgagct
     4741 ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc tatgctaagc
     4801 tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct tccttgttaa
     4861 tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa gatattgaga
     4921 cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta gctaacaaat
     4981 taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca ccaaagaatc
     5041 aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta
     5101 aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct cctacaacta
     5161 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
     5221 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
     5281 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
     5341 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
     5401 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
     5461 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
     5521 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
     5581 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
     5641 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
     5701 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
     5761 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
     5821 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat cgactttgat
     5881 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
     5941 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
     6001 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
     6061 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
     6121 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
     6181 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
     6241 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
     6301 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
     6361 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
     6421 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
     6481 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
     6541 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
     6601 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
     6661 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
     6721 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
     6781 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
     6841 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
     6901 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
     6961 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
     7021 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
     7081 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
     7141 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
     7201 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
     7261 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
     7321 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
     7381 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
     7441 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
     7501 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
     7561 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
     7621 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
     7681 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
     7741 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
     7801 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
     7861 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
     7921 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
     7981 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
     8041 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
     8101 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
     8161 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
     8221 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
     8281 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
     8341 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
     8401 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
     8461 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
     8521 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
     8581 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
     8641 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
     8701 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
     8761 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
     8821 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
     8881 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
     8941 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
     9001 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
     9061 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
     9121 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
     9181 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
     9241 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
     9301 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
     9361 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
     9421 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
     9481 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
     9541 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
     9601 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
     9661 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
     9721 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
     9781 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
     9841 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
     9901 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
     9961 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    10021 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    10081 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    10141 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    10201 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    10261 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    10321 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    10381 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    10441 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    10501 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    10561 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    10621 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    10681 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    10741 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    10801 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    10861 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    10921 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    10981 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    11041 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    11101 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    11161 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    11221 tggtgaatcg gcaatgaagc caacgttga
//
LOCUS       DARBER010000317        11249 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.087, whole genome shotgun
            sequence.
ACCESSION   DARBER010000317 DARBER010000000
VERSION     DARBER010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.087"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            255..1295
                     /locus_tag="SCK06_002933"
     CDS             255..1295
                     /locus_tag="SCK06_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1152840.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLISVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1393..1581
                     /locus_tag="SCK06_002934"
     CDS             1393..1581
                     /locus_tag="SCK06_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1152841.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1639..1992)
                     /locus_tag="SCK06_002935"
     CDS             complement(1639..1992)
                     /locus_tag="SCK06_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152842.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2523..2945
                     /locus_tag="SCK06_002936"
     CDS             2523..2945
                     /locus_tag="SCK06_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1152843.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2942..3172
                     /locus_tag="SCK06_002937"
     CDS             2942..3172
                     /locus_tag="SCK06_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1152844.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3670..3870
                     /locus_tag="SCK06_002938"
     CDS             3670..3870
                     /locus_tag="SCK06_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1152845.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3897..5090
                     /locus_tag="SCK06_002939"
     CDS             3897..5090
                     /locus_tag="SCK06_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1152846.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5158..6723)
                     /gene="guaA"
                     /locus_tag="SCK06_002940"
     CDS             complement(5158..6723)
                     /gene="guaA"
                     /locus_tag="SCK06_002940"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1152847.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6782..7705)
                     /locus_tag="SCK06_002941"
     CDS             complement(6782..7705)
                     /locus_tag="SCK06_002941"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1152848.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7901..8506
                     /locus_tag="SCK06_002942"
     CDS             7901..8506
                     /locus_tag="SCK06_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1152849.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8814..9830
                     /locus_tag="SCK06_002943"
     CDS             8814..9830
                     /locus_tag="SCK06_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1152850.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9971..>11249
                     /locus_tag="SCK06_002944"
     CDS             9971..>11249
                     /locus_tag="SCK06_002944"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1152851.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTL"
BASE COUNT         3683 a         1999 c         2236 g         3331 t
ORIGIN      
        1 gttatattat cataatacca caaaatcaca aattttcatt caattatcat tttcaattgc
       61 attttataaa tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca
      121 ttttgaagta cattttgtca ttttgtactc caaaactgaa ctacttactt gcatatatac
      181 gcctttatag tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa
      241 taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt
      301 attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaattagt gttcttggag
      361 atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa
      421 ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct
      481 cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt
      541 ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg
      601 tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag
      661 aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg
      721 tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact
      781 taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat
      841 tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtacggttta tactatagcc
      901 ctgttagtac tccagcagat tttcggatgc ttactcctat tgtactggag caagccttta
      961 gaaaagctgg aacagaattg ttagagccat atcttagttt taaagtttat gcaccacagg
     1021 aatatctttc acgggcatat aacgatgctc ccaaatattg tgcaaatatc gtaaatactc
     1081 aactgaaaaa taatgaggtc attattattg gagaaattcc tgctcgatgt attcaagatt
     1141 atcgcaatga tttaactttt tttacaaatg ggcttagtgt ttgtttagca gagctaaaag
     1201 gatatcaggt taccactggc gaacctgttt gccagacccg tcgtctaaat agtcggatag
     1261 ataaagtaag atatatgttc aataaaataa cttagtgcgt tttatgttgt tatataaata
     1321 tggtttctta ttaaataaga tgaaatattc tttaatatag atttgaatta aagtggaaag
     1381 gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca
     1441 agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt
     1501 aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa agagccagac
     1561 gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg ctctttttgt
     1621 ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac cttttgctag
     1681 tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt ctacctgtaa
     1741 ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa tagatacatg
     1801 gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt tgttttctat
     1861 atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac gagtgagacc
     1921 ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt atttatcttc
     1981 ttttttacgc atactaaatc acctctatac attttactgt tcctatataa ccataaacag
     2041 gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg aatttattaa
     2101 tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat acaactttat
     2161 aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga gggaaaagtc
     2221 gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc aaaaaaactt
     2281 atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac atagatagaa
     2341 aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg tcacttaata
     2401 aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg tcggggttta
     2461 gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag ggggtgaaaa
     2521 gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac
     2581 aagttattga agatgaacga aaagattact tcaaacatct agacagacta tcagcaaaag
     2641 aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact gttgatacgt
     2701 acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt gaaagtgatt
     2761 tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt ttacttcatt
     2821 actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat cgttcgactg
     2881 tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag
     2941 aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga
     3001 cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt
     3061 acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa ccctacgtgg
     3121 cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat agtcccattt
     3181 tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt tgttattcca
     3241 tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac gttctttgac
     3301 aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc
     3361 atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc tttagcagga
     3421 ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata gtccgagcgt
     3481 tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt gaaattcctg
     3541 tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt gtattattat
     3601 aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat aattgggaaa
     3661 ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata tggaacttct
     3721 ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg gcatatctac
     3781 tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat tctaggaatt
     3841 cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta aaaactatgg
     3901 ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca aatctaggtt
     3961 atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact aaaaaagaag
     4021 caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt gagaataagg
     4081 ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat aagacacagg
     4141 taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc tcttattttg
     4201 ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa ctaaaattat
     4261 caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca gttgctatgg
     4321 atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt ggcaatgtga
     4381 aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa gtaatctctc
     4441 taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg tttttattta
     4501 tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt gattttgact
     4561 caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat tatagatttg
     4621 ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc acattaactt
     4681 atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt gtaatgagct
     4741 ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc tatgctaagc
     4801 tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct tccttgttaa
     4861 tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa gatattgaga
     4921 cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta gctaacaaat
     4981 taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca ccaaagaatc
     5041 aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta
     5101 aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct cctacaacta
     5161 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
     5221 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
     5281 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
     5341 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
     5401 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
     5461 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
     5521 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
     5581 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
     5641 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
     5701 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
     5761 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
     5821 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat cgactttgat
     5881 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
     5941 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
     6001 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
     6061 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
     6121 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
     6181 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
     6241 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
     6301 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
     6361 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
     6421 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
     6481 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
     6541 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
     6601 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
     6661 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
     6721 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
     6781 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
     6841 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
     6901 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
     6961 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
     7021 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
     7081 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
     7141 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
     7201 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
     7261 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
     7321 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
     7381 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
     7441 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
     7501 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
     7561 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
     7621 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
     7681 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
     7741 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
     7801 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
     7861 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
     7921 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
     7981 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
     8041 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
     8101 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
     8161 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
     8221 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
     8281 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
     8341 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
     8401 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
     8461 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
     8521 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
     8581 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
     8641 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
     8701 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
     8761 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
     8821 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
     8881 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
     8941 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
     9001 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
     9061 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
     9121 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
     9181 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
     9241 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
     9301 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
     9361 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
     9421 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
     9481 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
     9541 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
     9601 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
     9661 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
     9721 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
     9781 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
     9841 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
     9901 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
     9961 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    10021 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    10081 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    10141 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    10201 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    10261 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    10321 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    10381 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    10441 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    10501 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    10561 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    10621 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    10681 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    10741 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    10801 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    10861 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    10921 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    10981 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    11041 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    11101 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    11161 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    11221 tggtgaatcg gcaatgaagc caacgttga
//
LOCUS       DARBER010000318         9961 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.097, whole genome shotgun
            sequence.
ACCESSION   DARBER010000318 DARBER010000000
VERSION     DARBER010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.097"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(121..993)
                     /locus_tag="SCK06_002945"
     CDS             complement(121..993)
                     /locus_tag="SCK06_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1152852.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1121..1690
                     /locus_tag="SCK06_002946"
     CDS             1121..1690
                     /locus_tag="SCK06_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1152853.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1757..2494
                     /locus_tag="SCK06_002947"
     CDS             1757..2494
                     /locus_tag="SCK06_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1152854.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2725..3993
                     /locus_tag="SCK06_002948"
     CDS             2725..3993
                     /locus_tag="SCK06_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1152855.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4480..4944
                     /locus_tag="SCK06_002949"
     CDS             4480..4944
                     /locus_tag="SCK06_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1152856.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4928..5557
                     /locus_tag="SCK06_002950"
     CDS             4928..5557
                     /locus_tag="SCK06_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1152857.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5911..7389
                     /gene="msr(C)"
                     /locus_tag="SCK06_002951"
     CDS             5911..7389
                     /gene="msr(C)"
                     /locus_tag="SCK06_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1152858.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7436..7882
                     /locus_tag="SCK06_002952"
     CDS             7436..7882
                     /locus_tag="SCK06_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1152859.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8081..8596)
                     /locus_tag="SCK06_002953"
     CDS             complement(8081..8596)
                     /locus_tag="SCK06_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1152860.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8713..9915
                     /locus_tag="SCK06_002954"
     CDS             8713..9915
                     /locus_tag="SCK06_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1152861.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3179 a         1748 c         2072 g         2962 t
ORIGIN      
        1 ctggaattat cagaaataaa aggaaggaac aaattaagaa acttatgtta tttaatcatt
       61 ggatcttttg cttaacttat tcgttgttaa aaagctgaaa gcgaaggaaa aaaactctac
      121 ttaactgtat ttctccgtta ggacatctaa aaatttacga gcagcaggag aaagaggctg
      181 gtcttttttc caaatgagat tcagtgaagc ttgtaatggc ggatcgagtg ggatgaatac
      241 tttttccgtc ccataagtat tgatgatccc atctagagct aatgcaccaa aagtcccgtt
      301 atcgaccatc aaagaagcat tgtacaataa gttatatgtt ccgacgatcg ataactgatc
      361 tatgctgaaa cctagccatt cagctagttg actgtccact gacgtttgcc gggaaatgat
      421 caaaggaaca gttttcagtt cctgcggatg gatgactgtt tgctttgcta aaggatgatt
      481 ccgatgtatc aaaagtcccc aacgatcttg ggctggcagt cgaaatgact ggtatttttg
      541 tttgtttgtc ggttcgatta cgagtccaaa atcaagtaat ccgctatcca gtttgtctat
      601 gatatcatct gcattcccgc tatataaatg aaactggata tcaggatgcg ccttgatcaa
      661 cgaatgaagg ctatcagaaa tgaaagagat tgctttagtt tcgcctccgc caatgtagat
      721 ttctccagag atgattgcgt catttgttaa attgttcgcc gttttttcta ccaacgacaa
      781 gatttctttt ccttttgaat aaagatatcg accagaatcc gtcaactgta tctctcggga
      841 gcctcgagtg aaaagtgtca caccaagttc ttcttctaaa tctttcagtt gttttgagag
      901 agtaggttgc gtgatatgca gagattttgc tgctcggctg atattttttt ctctagctac
      961 tgctaaaaaa tacgtcagta tacgaatatc cattttttcc ctcctgtttt taaaaaagca
     1021 taccactaag tatagttaaa aggaatagtt aagtatgttt tataagtatt agacatcata
     1081 agccgtccgt tataaagtaa ggataaaagg agggagcaaa atgacaagcc tcatcgaaaa
     1141 acaaatcgtc aataaagaga ttcgagtaac ggatccgctc tttgaagtga tccatcagat
     1201 ccaagcggaa aacgaagagc ctttagcagc gctgaatact gggtaccatg aaccagcaga
     1261 tatccgcaaa cggctagaaa atatcattag tgacaaagta gatgatacgg tgactgttct
     1321 gctgcctttt tatacagatt tcggcaaaca tattagtata gggaaaaatg tctttatcaa
     1381 tcgtcaagtg atgttcgttg acttaggtgg catctgttta gaagattcag tattgattgg
     1441 tcctcgtgtc aacctcatca cggtgaacca tctaaccgat ccaaaagaac gaagaggctt
     1501 gtcagtaaag ccaatccaca tcaagaaaaa tgcttggatc ggagcaggag caactattct
     1561 gccaggcgta acgattggcg agaatgccat tgttgcagcg aatgcaactg ttacgaaaga
     1621 tgtcccagac aataccatcg ttgcaggaat ccctgccaaa atcgtcaaac ctgtcgaacg
     1681 gattatttga acagtaagat gaaataagag atagtgagta taagcaagag atttttcaaa
     1741 acaggaggag aatagaatga atgatttatc agaaaaagtg attgtcatca tgggcgcttc
     1801 aagcggaatc ggtgaagcaa caacgaagct attagcagaa aaaggggcta aactagttat
     1861 agctgcacgc cgagaagatc gcctaaaagc aataaaagaa tctttaccag aagctgagct
     1921 atatattcaa acagcagatg taagagattt tgcacaagtc caagcagtta tcgatttagc
     1981 gatggaaaag ttcggtcgaa tcgatgtatt gtacaataat gcaggaatca tgccaaccgc
     2041 tccattagtt gaaggtcacc gtgatgaatg gcaaaatatg cttgatatca acatcatggg
     2101 tgtgttaaat ggaatttccg ccgtacttcc aatcatggaa aaacaaaaat ctgggcatat
     2161 catttcaact gattctgtgg cgggacatgt tgtttatcct gattccgccg tttattgcgg
     2221 aacaaaattt gctgtccgtg caatcatgga aggattacgt caagagcaac ggcaaaataa
     2281 catcaaatct acgatcatct ctccaggagc tgttcaaact gaattatatc aaaccatcag
     2341 cgacaaaaag gctgctcttg aacttcatga agcgcaaaaa gaatggggat taacatctga
     2401 agatatagct tctgctgtag catttgcgat agaaacgcca gaccggatgt ctgtcagcaa
     2461 catgattatc cgtccgacga cacaggaagt ataatccata acggataata tccagagaga
     2521 attacttgtt aaatagttat tcaaacgacc atcaatttaa ggcgtgaatg gagaagaata
     2581 ttcttcattt acgtcttttt atgcagaaaa agcaaattta ttaaaaagaa acaaatgtta
     2641 tttaaatatg gacattgact tgcttttact tttttagtta cactgttatt taggaaaagt
     2701 ttcataattc aaaggagaaa aaagatgaaa gcaaagaatt tattatttag cgttattctt
     2761 ttaggaaatc tagcagttgc caacacaaca gtgctagctg aagaatcgac agaaactgtc
     2821 gcaactcaga catcgagttc ttcagaaaca ggaattgaaa acgatacaga aagtactttg
     2881 cagtcagcta ctgtctcaga aaatacaatt tcaaccgaag aaccaacaac tgaaacaaca
     2941 gaacaaacac aaaccgaaga cagtacatca gcagaagctc agaacaatcc agagaatgca
     3001 gaagaaatca cgaaccaatt gacagatggg caaacattga cacataccca tcaagcagat
     3061 ttgtctgaat tcgaaaacta tactggctat gctttttatg ctcgctcatc tgctaattct
     3121 caacaagcgt tcattgatag tattgcctca actgcacaaa gtttggcaag tgccaatgac
     3181 ttatatgcat cagtcatgat tgctcaagcc atcgttgaaa gtggctgggg gaacagtact
     3241 ttggcttctg cccctaatta caatctattt gggattaaag gaagctataa tggccaatct
     3301 gttacgatgc ctacttcgga atatgtgaat gggcaatgga ttacggtgaa tgccgctttt
     3361 agaaaatatc cttcgtacaa agaatcattg caagataacg taacggtatt gaagacgact
     3421 tcttttcagc caggcgttta ctactattcc ggtgcatgga aaagcaatac gaactcttac
     3481 aaagatgcta cggcttggct aacaggtcgt tatgccactg cgcctaatta tggttctaca
     3541 ttgaacaatg taatcgaaac ctataatctg acacagtatg atacagcacc aacttcaact
     3601 tctccgatgt atcgtctgta taaccgtcat acaggagaac atttatatac attgaatgcc
     3661 ggagaaaaaa attatctgcc aactgtaggc tgggaatatg aagggattgc ttggcaagca
     3721 ccaaacagcg gacaacctgt ctaccgttta tacaatcctt acagtggaga ccatcattac
     3781 acaatggcac aatctgagat caactcttta acaaaaatcg gttggcgtta tgaaggtcta
     3841 agtttttatt ctggtggttc caagccaatt tatcgactat ttaatccaaa tgaaaaaact
     3901 ggtacccatc actatacctt gagtgctaag gaaagagatt ggctaacacc gatgggctgg
     3961 agatatgaag gaataggatt ttatggttat taaaaagagg tagtgaaaaa gctgccctgc
     4021 atcaagtagt aagaacagcc aaacaaaaac agattttcat gttttttgtt tggttgaact
     4081 tattaccgca gatgcaagtt tttgaacacc gtttactcaa aaaaggttgt gacaacatct
     4141 tgtcataact tttttattac tgtttaagca tactgatcca atttctaata ttcgacggga
     4201 ttcttgcaaa aggcgatttc ggaaattttt ggcggtgtaa aattatctac tgcagaagag
     4261 ctatcttcta tagtttgata agaatcaata atcttggttt tagcgttgtg ctttctttat
     4321 cattttttcc gttcattcta caaaaaattt cttttctcta tacttttcta ttgagagcgt
     4381 tttttttatt acacagctga ttctttgact tttttctttt tttattctat tattgacaag
     4441 tcaatcgttg acgagtcaat tattagaaag gaggaaatta tgtctttaga agagattagt
     4501 caccttttat atcaaatcaa attagccgat caaaaaatcg tccagatctt tgaagaaaat
     4561 atcggtataa gtcttactag atacgagatc atgatgagac tgaaagaaga aaatccattg
     4621 ctacaaagca gtttgcaaga aaagttgcag attgatcaag gagcagttac tcgtcatctg
     4681 aaggtattgg aagcaaatgg ctatgtttcg cgtcagagaa atcctcagaa caaccgggaa
     4741 gtgtatgtag cgctaacaga aaaagcccag aagaaaatca agcattgtga aatggaccat
     4801 aaagatacag gttccgtgct atccccggct ttcagcgatg tagaaatgaa acaatttcta
     4861 acacttttag ataaattgaa tacccaactt tgcaatataa caacagaaac gaaagaggtt
     4921 aataataatg gaacaaaaaa atgatttttc aacaattatg actggtagaa gatccgttcg
     4981 cgtatatgat gaaacataca aaatcccaca cgaagaaatg ctggaaatga tcaaagaagc
     5041 gacgactgca ccttcttctg tcaatatgaa accatggcgc tttgtggtcg ctgaaagcga
     5101 ggaagcaaaa gaaatcttgc gtcctttgat ccgtttcaat acgagacaaa atgatacttc
     5161 ttctgcaatg gtcatgattt ttggcgatat gcaatgttac gaatatggcg aagaaatcta
     5221 caaccaagct tacgaatcag ggaagatgcc aaaagaagtc agagatcaac aattagctgc
     5281 gatcattcct tattacaaga gtttttcaag agaagaaatg aacgatgtag tcaaagtcga
     5341 ttctagctta gcggctatgc aattcatgtt agttgcgcgt gatcatggct atgaaacaaa
     5401 tccaattggt ggattcgaag cagatcagtt ggcagaggca tttggtttgg ataaagaccg
     5461 ttatgttcca gttattattt tatcaatagg aaaagcggta gaagaaggtt acgagtctgt
     5521 tcgtttagca ccagaaaaga tcactatgtt cgaataaaag gttttcagtt actattcagt
     5581 agctattgct ttttttgaat cgtgtgtggt aacctaataa aaaaggaggt cggaaaaatg
     5641 actgcatcga tgaaattacg tttcgaactt ttgaataaca actaaataaa ttcaaaagac
     5701 atgtataaac aacaatttca tggatggagt atgtccattt tttcgaacga cagtagaata
     5761 ggtgaaagct atgacagatt tggtgattta gtcatagtta tttcgttcag agtaggaaaa
     5821 gatgtgatgc agtttttttg caaacagatt tttctgctct tttttagtat cgataattgt
     5881 tgttttacat gaaataggag tgtaaaacat atggaaaatt tagcagtaaa tataacaaat
     5941 ctacaagtca gctttggcaa ccagctagaa ctatctattg attctcttcg tgtctatcag
     6001 caagatcgta tagggatcat aggagaaaat ggtgtcggta aatcgacttt gctcaaacta
     6061 atagccggtg aactttttcc cgatcatggg aaaatccaaa cagagatcac cttcaactac
     6121 ctgcctcaat taacctatct cgctgaagca aaggacctaa atttggaatt agccagtcat
     6181 ttccagttaa gactggaaga aacttcagag cggaaatgga gcggagggga agaacgaaag
     6241 atcgagttga tacgtcttct ttcttcttat gaacaaggga tgcttctaga cgagccaaca
     6301 acccatctag atagaaaaag tattgatcga ctgattgaag agcttcgtta ttattatggt
     6361 acgctggttt ttgttagtca tgatcgctat tttctagatg agctagcatc gaaaatctgg
     6421 gaagtaaaag acggagaaat ccgagagttt tcggggaatt atagtgccta tctcactcaa
     6481 aaggaattgg agaaaaagac tcagctacga gaagcagagt cgatcatgaa agagaaaaaa
     6541 cgattggaaa aatcgatcca agaaaagaaa aaacaagcgg aaaagttgga aaaagtgtcc
     6601 agtaaaaaga aaaagcaaca aatcagactg gatcggttgt cttcctctaa acaaaaagac
     6661 agtgtacaaa aagccatcca aaagaatgcg aaaacattag agagaagact ccaaaaaata
     6721 ggagaaacaa ccaagccgca gcagatgaaa caaatccgtt ttccagtacc aaaatctctt
     6781 gaactccaca gccgctatcc catcatggga caaaatgtcc aattggaaag aagtgggaga
     6841 acattactgg taaatggtga ttttcagttt tctttaggta aaaaaatcgc gattgtcggt
     6901 gaaaatggtt caggtaagac aaccttatta gaacatatcc gcaaacaagg agaaggaatc
     6961 cttctctctc cgaaagtaag ctttcaagta tatcagcaaa agggttatca aatgacatct
     7021 gaagaatcca tcattcgttt tgtcatgaga caaacagagt tttcagaatc gcttgtccgt
     7081 agtttgctga atcacttagg gtttgctcag gaaactttga cgaaaccgtt atgtacatta
     7141 agtgggggag aagcgacccg tctgacgatt gctttgcttt ttactaagcc aagtaatgtg
     7201 ttgctgttag atgaaccgac taattttatt gatatggcaa cgatcgaagc tttagagaag
     7261 ctaatgcaaa tatatccggg aacgattttg tttacttctc atgattctta ctttgtcgag
     7321 cgtacggctg atgaagttta tgaaataaaa gggcagaaaa taaaaaaagt acttacgaga
     7381 aatttttaaa tgtgctacga tgattttcgt gacaaattta aaggagtggt acagattgcc
     7441 tacaaataaa aaagaaggaa taatttttac aacaatgatg tgttttttga tggtcttggg
     7501 gatgagcgcg tataatttat ggctgcatca agatcttcat ttatcaagtt tgttgattgg
     7561 tctagtgcct ggattcgtgg tggctttcat tttggatgtc ttcatcatcg gtgtgctggc
     7621 gaaaaaaatt gcctttaaac taccagtaaa tccagaaagc aaggtccaaa tgatcttaac
     7681 tatttcatgc ttgatggttt taggaatggt gacatgtatg tctttattcg gtgtgatcat
     7741 ggaacaagga atacctgatc aattattctc tgcttatatg aatgcttgga aaatgaatgt
     7801 gattgccgct ttaccgttgc agttgatcat cgttggcccg ctttcaagaa gtgtgctaaa
     7861 agcaattcaa agtaatgaat gatggaacac tgttatttga aaaaaggcct tggcatgctt
     7921 tttgtcatag tctcttgaat gcagttctag aaaaatagtg atccgaacga taggttttgg
     7981 cctgtcgttc ggatcgcttt ttcttttcca actgtattgc tcttttattg aagataattt
     8041 gctcttcaat agatatttct agaaaggttc tgtttatccc ttactcaaaa aactgctgca
     8101 tttgttctct tacttgctga caccattgga gttcctgctg acaaagcata attctgcgct
     8161 ggatcacaac ataacgtcca aattcttgtt tcttgtctag cgagaggtca ttcaattttt
     8221 tttgattttt ttgcatgatt ttgttcagct gttgttctct agtgaatagc agtccagtag
     8281 ctgttgtttt atcgagcgtc gaaaaagcat agatttttgc tagaaattca tctttttgcg
     8341 taggagcggg agtttcctct ttgatccatt cctctacaag agcacgtcct tcatcagtca
     8401 agtggtagat ctttttttga gagtgctcgt ctttgtccaa aacggtcaca taaccctgct
     8461 ggtctaactt ggcaagcgtt gtatagatct gactgtgatg ggcttcccaa aaaattgtga
     8521 tcagctgctt caattcatac ccagaacgtg gtttggataa aagcacgcat aataatatat
     8581 aggctaaagt attcataaaa atcctacctc tcgtcttatt ttctttatta tatcacagta
     8641 aagtttccac gttttacatg tgcatattga catgtaaaat aaatcatgta tgatgctaat
     8701 agagagaaag ggatggaaat attggagaat caaatagata tacaagaaca aacaaatgaa
     8761 gaaacaaagg ggaaaagttc agcgctagtt gtagcagcta cctgtatgat cgcttttatg
     8821 ggagtgggtc tagtagaccc aatcttaaaa acgattgctg cacagttaaa cgctacgcca
     8881 gctgaaacga ccctgttatt tactagttac atgttagtca ctggtgtggt gatgctattt
     8941 acaggtttct tatctagtcg gatcggtttg aaaaaaacat tgatgacagg attgtttatc
     9001 atcgttgtat tcgctggtct tggcggatta tctgggaata tcggaatgct gattgggtta
     9061 cgtgccggct ggggtgtcgg gaatgcttta tttgtctcta ctgctttagc aacgatcgtc
     9121 agtattttag ttggtaacac agaaaaagca atcatgatgt atgaagcagc acttggctgc
     9181 ggaatggctg ttggccctct agtcggtggg gtcttgggca gtggttcatg gcgttatcca
     9241 ttttttgggg tagcattttt gatgttttgc ggttttatag ccctagcggt acttttacct
     9301 gaaactgaga aaccaaagag aaaagtaggc ctttgggaag gtgtcaaagc gttaagcaat
     9361 cataaacttc gttcagttgg actaatcgca ttgttatata atttcggatt ctttacttta
     9421 cttgcgtatg cgccattttt attgattggc tattctgaat tagaagtcgg ttttgtattt
     9481 tttggctggg gtgtgcttct ggctattgct tcgattttcg ttgcaccggc tcttgagcgg
     9541 aagtttacga cttatcggac aattttagca gcatttgtct tattcattat ttgtttgctg
     9601 ttattaggag ctggaacttc tatgccagtc cttgtaccga tcagcattgt gttagcagga
     9661 tttttccaag gaattatcaa tacactgttg acgacgattg ctatggaaat accaggtctg
     9721 caacgaaacg ttgcttcgtc cagctatagt tttgtccgct ttttcggtgg agctttagca
     9781 ccatttattg caggtgtgat tggtgaacat attggtgaaa gctatgcgtt ttattttgca
     9841 ggtgccattg ttcttatcag tgtatgttgt atcatttatc accgtaatta ttttgctaca
     9901 aatgaagaag aataagaaat cagaggttgt gaaaaagctg tcctgcatca agtcataaga
     9961 a
//
LOCUS       DARBER010000319         5613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.148, whole genome shotgun
            sequence.
ACCESSION   DARBER010000319 DARBER010000000
VERSION     DARBER010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.148"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(40..1026)
                     /locus_tag="SCK06_002955"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="SCK06_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="SCK06_002956"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="SCK06_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1152862.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="SCK06_002957"
     CDS             1851..3005
                     /locus_tag="SCK06_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1152863.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="SCK06_002958"
     CDS             3220..4188
                     /locus_tag="SCK06_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1152864.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="SCK06_002959"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="SCK06_002959"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1152865.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="SCK06_002960"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="SCK06_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DARBER010000320         3307 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.182, whole genome shotgun
            sequence.
ACCESSION   DARBER010000320 DARBER010000000
VERSION     DARBER010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.182"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            126..623
                     /gene="dfrG"
                     /locus_tag="SCK06_002961"
     CDS             126..623
                     /gene="dfrG"
                     /locus_tag="SCK06_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1152866.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(695..2647)
                     /locus_tag="SCK06_002962"
     CDS             complement(695..2647)
                     /locus_tag="SCK06_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1152867.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2654..2890)
                     /locus_tag="SCK06_002963"
     CDS             complement(2654..2890)
                     /locus_tag="SCK06_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152868.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3125..>3307)
                     /locus_tag="SCK06_002964"
     CDS             complement(3125..>3307)
                     /locus_tag="SCK06_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1152869.1"
                     /translation="FQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCG
                     NVFKIEYTKKRQRLAY"
BASE COUNT         1016 a          578 c          546 g         1167 t
ORIGIN      
        1 gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg
       61 agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt
      121 tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga
      181 atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac
      241 atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa
      301 gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt
      361 caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag
      421 aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc
      481 atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat
      541 ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg
      601 aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag
      661 cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt atatctttcc
      721 aatcaaattg tttatctgta atccctaatc tttgagcagg tgtcaaaacc tttttatccg
      781 ctgatttaaa agggcgacaa aagttgtagt aagttcttag aattgtaatc gcaaattgag
      841 catacttcgg attgaagtta gcgtaaatat agctcttctt atcaccacga gctgttgtta
      901 atggtctttc aagagatgat atataacgtc ttatctgttg aataaagcta ttggtcgaat
      961 ggtcattaac atttaatagc atctttgcga tttcgtttgg ttccagagca gataaatccg
     1021 ttctacaatc aactgaataa aatcctttat ccttagtggc taatggatgt ttgatagggt
     1081 tatcggcata tttgcgatat ctttctccat ctttactaat agcctcttcg tggaattgat
     1141 gacttgttag taattcagtt agataatgag atgccaaagt ccgtagaaac tttgttttga
     1201 atcctctgat atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat tcttttaagc
     1261 attgttttct gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca tcgctcaacc
     1321 ttaattcttt cgtgaatact ctataaaagg cgtttctaat agagaagtca tcatcactaa
     1381 tcattcgcca ttcgtcggca ttaaccattt gtttaattaa ccaatagtga gcagttgtag
     1441 tataaggagc atcaacattt aaaccatcga caaatctact cctattagta attttcccta
     1501 attcgtgaag ataagcattc ctattttcgc tatcgttagc ggatggttct tgtggataat
     1561 aagaccaatc taacctatca tttttacgac taaaagtatt gagatggtct tctttgaatt
     1621 ttcttgtatc ttcatttaat tcatccattg aaatattcca atcataggca acatcagaac
     1681 ggaacacata tcttgataag acctcagcag atacaactat atacgtttgc atattaaggt
     1741 cttcaaaccc cgtatatttc tttgaacctt gtcctttctt acggacgtta tttaggtagt
     1801 agtgcatctt gtccgtatta agccacatct cgttaaattt ctttgtttga aggggttgtg
     1861 tctcatatcg ttctaaaaac tccaaacaac gcctatacag ccactccagc ttatgatagt
     1921 aggttccaac tccaattcct aaaatatcac aagtacgact gactgaaacc cttccaacaa
     1981 ccatcttagc gaacattgga agaatggtgt tcttttgttg gtgataagta gtggtttctt
     2041 ctctttttgg tagaacatta gtgaacttct tacacgcctt acattgatag cgttgagatt
     2101 tgcccctact tttccccctt ttgtaaaatt gcttcggctc attgaatggg gttgattctt
     2161 cttccgagca gccttcttta tgaaattgat aatctggctc aacatcttgg atgctattga
     2221 tttctataag gcgtttaatt tcttcaacaa cagaccaatt agataaaggg gttactgtac
     2281 agttttgcga tacacctctg ccaataggat tgtcattgca gtacatacct ttatgacctt
     2341 tatctttgga actaccagtc attttatatc gacttggttt cccttttaca tctttgaatt
     2401 tgtgctgttc tttcccaaaa ttacaacaaa acggattgat gcagtaatta tattgaatgg
     2461 gatattcaga accgttccat ttaaaagacg ttggcaagaa caacattttg ttataccttg
     2521 ttgcaaatcg ttttggagtt aataaatcat attgtttagc cctatctttt aattcttcgt
     2581 ttgaaatcgg aattttaaca tctacaattt catcttcctt cgtggctaat cttttaagct
     2641 tggtcattgt ctatcaaccc actctatctt tgatacccat tttttcaaca atccgcttat
     2701 tacttcgctt gttggcaatc cattcaataa acttcttatc gtctaaaatg ttcaataaga
     2761 attcatccac tgcttcgctt tcagtacgct ctgcatattc agcgtattgc ttaactatca
     2821 cccttacttg ctctgatatt tcccaatcca cagacttagc gttcttattt tttggctcaa
     2881 gaaattgcat aggaaaatgc accccttttt gcttagtaaa ttgtaaccat tttaacacac
     2941 caatttgcat tcctacaata attatttcac gttcgcattt ttatatacat tttaacttac
     3001 attttgtggt gtaaattaac aacaaacgtt gatttaacaa taaaaaacga gtatcaaatt
     3061 cagttgcaat ttgatactca tttaggttta cattttcact ttttgtactc caaaactgaa
     3121 ctacttaata tgcaagacgt tgtctttttt ttgtatattc aattttgaaa acatttccgc
     3181 aaagttcaga cggacctcga tgtgttgatg aataaaattt attagtaata acttctataa
     3241 ggttatcaat ccctatatta ctttttgcac ttccatgata aagagggaac agagaacaat
     3301 tctgaaa
//
LOCUS       DARBER010000321         3178 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.188, whole genome shotgun
            sequence.
ACCESSION   DARBER010000321 DARBER010000000
VERSION     DARBER010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.188"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1108)
                     /locus_tag="SCK06_002965"
     CDS             complement(<1..1108)
                     /locus_tag="SCK06_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152870.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLS"
     gene            complement(1822..2112)
                     /locus_tag="SCK06_002966"
     CDS             complement(1822..2112)
                     /locus_tag="SCK06_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1152871.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(2320..3012)
                     /locus_tag="SCK06_002967"
     CDS             complement(2320..3012)
                     /locus_tag="SCK06_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1152872.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1002 a          573 c          443 g         1160 t
ORIGIN      
        1 aacttaaata aattccaaca attaaacata gtaataatgc caagactact aaagtagcta
       61 tccaagtacc tggataaaaa agtgacagtt tcgttcctaa ctctgtaaag agattattca
      121 ggttactact actccaaatc attgcccttt tatttccatt gacgtaagta aagctttgac
      181 ccattgtatt accaaaataa ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac
      241 gcacataata gttttgacat ttcctatatg ttagaaggaa aattaagaaa aatagcaata
      301 aaaataagat attcatgtct aaaaaaaacg gctttccaaa tgatgaaata cctaaatcta
      361 acttattaag aaagttgaaa tttttatatt caggaaacca tttattaatt tcatgtgcag
      421 cacgtaatgc attccctgga gttgtcaaag agaaaattaa ttctgctaag cttattattg
      481 aaaatactga taacttataa ttatatcgtc cattgacaat caaatataaa ctaactatac
      541 ttgttagaac aaaaaaacag gcgttgactt gttcttgatt agttaaaaat atcaataaag
      601 gaatacttac ccaatatatt tttctattta tttcttctcc tcttaagagt tgaaaaaacg
      661 gataaaaagt tagtaaacta aatgctactg gccactgata gttcaaggtg gttgctctcc
      721 aaccggtttc ccccattact gtcaatggta ttaaacagta tatagagcaa accaataata
      781 aatttttaac atttattttc tcaaaaacat acttgcaaag taatactaca attcctaaca
      841 taatcgtaga atttaataat ttccatagca taaagtgttt agaaaagaac attaagaata
      901 gttctataat aattcttgaa ctccatgtct tataatgttc tatcgaagca cccaaatagc
      961 caagttcatc taatctactt gcataaacta cgtcatccgc tctcatagtc ggcatataca
     1021 tattaaagag taaaaagata ccccaaatcc ctattagtaa caagatcagt tctttgttat
     1081 tcgttatttt tctattatgt ttgttcattt ttatactcct tcttatattt cctaatagag
     1141 tataacatag ctttaagcat tacttaaaga taacttaaaa gttatctttt aaatacttaa
     1201 ttgttaaata ccttatttga tgcaaaagaa atctacgata gtcaacaaaa aaattttcat
     1261 tatatccaga aaaatttttc ttagttaagg acctatggat tgcagtaatg cagaattcgt
     1321 cgaataaact gccaaagaca tacaatcata ttgcttttaa aattgacgaa caagatatag
     1381 attcgtttgt ctcaaaaatc caaatgttag gtttaactgt cgaaccagga cgttctcgtg
     1441 tgaaaggcga ggcttcttca atttactttt acgactatga taatcacctc ttcgagttac
     1501 ataccggtac tctccaggaa cgtttaaaat cctacaattc cactacctga gactagtaat
     1561 gactagtctc tttttatggc ctctgatccc cccattgagc gaatactaat agggcttcga
     1621 attgatctgt tcaatcttta agcaatatcc ctcaaatgag tttggaaagt ttagtgattc
     1681 atcacaaata cttaaataca tgtagttata tatagattga ggttcttgtg agcgtatgct
     1741 cgtgtgggtt caaatcccat cgtctgcatc catctttcca aaacaagcgg ctaggacatt
     1801 tttgtctcag ccgcttgttt tctagtttat tctttcatct tgtcggacct tttttcttgg
     1861 gtatagactt ttctcatatt ctaccacaat tttgggtttc cctaacacat gataggatct
     1921 cacaacaaat tgctttctat tattataata acctgtaacg actaacttca tcccttcgtc
     1981 tacttcagct aggaagttca aactatggga agcaatcaaa caattgaccc catttaaact
     2041 gaaacggatc aacggtctgg cactaacttg caaagtacgg atcttatcta ctgttccata
     2101 ttttttctcc ataaccttca cctcaatacc attatacgaa catacattcc ctttgtaaag
     2161 aagaaactct gttttatctt tttgactttt tgtctattac ctttcttaaa atcatattgg
     2221 cacttcctgc gaagatgaag gacatgtact aaaaaaaaga ggcagcagta tagattactg
     2281 ttgcttcttt actaatttag gtttacttgt taagaaagat tatttacgca aatgttcttc
     2341 aacaatttgc tgagatttat cagctgtatc ttccacttca tcttttgctt tttctaactt
     2401 ttgaatatat tgtttcaagc ttgagttttc agcagttgct tcgtctaatt tcttttgtgt
     2461 atctgttaac tgagtttcta catttgtttt ttcttgtgtc agtgttgcga tttgttgatt
     2521 caaaccattt acagtatttt gtaattctga aagttttgct tgtgcttctt gtagtcttgc
     2581 tgtccaatct gcattcagat gattgatttc atcatttttt tggttgatct catctagttt
     2641 gacattgatt tcattttgtt tgctttgtag ttgtgcatcc ttgctgacta attgactttc
     2701 taagttttgt ttttctgctg ccgattgatt tttttggttt tctaattcat ttacttttgt
     2761 ttggtattct tggacatctg cctttgaatc ttgcaatgtc tgttcttttt gtgctagtcg
     2821 agcggtaaga tctgtaagcg cttgctcttt atctgtcaat gtatgatcaa gctgtgtcag
     2881 attctcattg attgtatcaa tatctgtctc tccaccccac aaaataattt catttgcgta
     2941 ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg acaatcccac cagtaataaa
     3001 tttctttttc atttgcatag ctccttaatc caatactttt acatgctaaa agtaagtgat
     3061 ctcaaacaca atgtcaagcg tttttaaatt acatgtgtaa ttttaatata aatactatca
     3121 aatactcatt taatcctcct tttttatcta tttaaaaaat cttttttata gagaaaaa
//
LOCUS       DARBER010000322         1504 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.232, whole genome shotgun
            sequence.
ACCESSION   DARBER010000322 DARBER010000000
VERSION     DARBER010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.232"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            262..345
                     /locus_tag="SCK06_002968"
                     /pseudo
     CDS             262..345
                     /locus_tag="SCK06_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="SCK06_002969"
     CDS             394..477
                     /locus_tag="SCK06_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1152873.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="SCK06_002970"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="SCK06_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1152874.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DARBER010000323         1421 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.239, whole genome shotgun
            sequence.
ACCESSION   DARBER010000323 DARBER010000000
VERSION     DARBER010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.239"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1350
                     /locus_tag="SCK06_002971"
     CDS             <1..1350
                     /locus_tag="SCK06_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEG1152875.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          114 c          239 g          471 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
     1381 gacggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
LOCUS       DARBER010000324         1349 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.245, whole genome shotgun
            sequence.
ACCESSION   DARBER010000324 DARBER010000000
VERSION     DARBER010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.245"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            46..297
                     /locus_tag="SCK06_002972"
                     /pseudo
     CDS             46..297
                     /locus_tag="SCK06_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="SCK06_002973"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="SCK06_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1152876.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DARBER010000325         1126 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.260, whole genome shotgun
            sequence.
ACCESSION   DARBER010000325 DARBER010000000
VERSION     DARBER010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.260"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            191..>1126
                     /gene="vanY"
                     /locus_tag="SCK06_002974"
     CDS             191..>1126
                     /gene="vanY"
                     /locus_tag="SCK06_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG1152877.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          406 a          157 c          227 g          336 t
ORIGIN      
        1 aaattcagca tgggtatcac ttatgaaaat tcatctacat tggtgataat agtaaatcca
       61 gtagggcgaa ataattgact gtaatttacg gggcaaaacg gcacaatctc aaacgagatt
      121 gtgccgttta aggggaagat tctagaaata tttcatactt ccaactatat agttaaggag
      181 gagactgaaa atgaagaagt tgtttttttt attgttattg ttattcttaa tatacttagg
      241 ttatgactac gttaatgaag cactgttttc tcaggaaaaa gtcgaatttc aaaattatga
      301 tcaaaatccc aaagaacatt tagaaaatag tgggacttct gaaaataccc aagagaaaac
      361 aattacagaa gaacaggttt atcaaggaaa tctgctatta atcaatagta aatatcctgt
      421 tcgccaagaa agtgtgaagt cagatatcgt gaatttatct aaacatgacg aattaataaa
      481 tggatacggg ttgcttgata gtaatattta tatgtcaaaa gaaatagcac aaaaattttc
      541 agagatggtc aatgatgctg taaagggtgg cgttagtcat tttattatta atagtggcta
      601 tcgagacttt gatgagcaaa gtgtgcttta ccaagaaatg ggggctgagt atgccttacc
      661 agcaggttat agtgagcata attcaggttt atcactagat gtaggatcaa gcttgacgaa
      721 aatggaacga gcccctgaag gaaagtggat agaagaaaat gcttggaaat acgggttcat
      781 tttacgttat ccagaggaca aaacagagtt aacaggaatt caatatgaac catggcatat
      841 tcgctatgtt ggtttaccac atagtgcgat tatgaaagaa aagaatttcg ttctcgagga
      901 atatatggat tacctaaaag aagaaaaaac catttctgtt agtgtaaatg gggaaaaata
      961 tgagatcttt tattatcctg ttactaaaaa taccaccatt catgtgccga ctaatcttcg
     1021 ttatgagata tcaggaaaca atatagacgg tgtaattgtg acagtgtttc ccggatcaac
     1081 acatactaat tggttctgtt gcaaagtttt aaatctacta tcaaat
//
LOCUS       DARBER010000326         1034 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4038
            SAMEA114569843-rid20112193.guided.267, whole genome shotgun
            sequence.
ACCESSION   DARBER010000326 DARBER010000000
VERSION     DARBER010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569843
            Sequence Read Archive: ERR12203668
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972619.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972619.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,975
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 8 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569843-rid20112193.guided.267"
                     /isolate="VRE4038"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            319..867
                     /gene="aac(6')"
                     /locus_tag="SCK06_002975"
     CDS             319..867
                     /gene="aac(6')"
                     /locus_tag="SCK06_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1152878.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          367 a          147 c          210 g          310 t
ORIGIN      
        1 ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt
       61 gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca
      121 gagttttttc ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct
      181 gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat actgattggt
      241 agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa
      301 gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa
      361 gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga cagctcggca
      421 gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat
      481 gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat
      541 ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac
      601 ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta
      661 gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct
      721 tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc
      781 gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg
      841 attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc
      901 aagaaataag aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat
      961 ttccgaagaa gctacttttg gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt
     1021 tcgagtagta agaa
//
